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- EMDB-35216: Yeast 40S-eIF4B - partially open conformation of the 40S head -

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Basic information

Entry
Database: EMDB / ID: EMD-35216
TitleYeast 40S-eIF4B - partially open conformation of the 40S head
Map dataThis is the cryo-EM map of the 40S-eIF4B complex from yeast.
Sample
  • Complex: The binary complex of the yeast 40S small ribosomal subunit with the eukaryotic initiation factor 4B.
    • Complex: Eukaryotic Initiation Factor 4B (eIF4B).
      • Protein or peptide: x 1 types
    • Complex: yeast 40S small ribosomal subunit
      • RNA: x 1 types
      • Protein or peptide: x 33 types
  • Ligand: x 2 types
KeywordsRIBOSOME
Function / homology
Function and homology information


endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / translation regulator activity / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rescue of stalled ribosome / 90S preribosome / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / small-subunit processome / positive regulation of apoptotic signaling pathway / protein kinase C binding ...endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / translation regulator activity / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rescue of stalled ribosome / 90S preribosome / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / small-subunit processome / positive regulation of apoptotic signaling pathway / protein kinase C binding / modification-dependent protein catabolic process / rRNA processing / protein tag activity / ribosome binding / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / small ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / cytosolic large ribosomal subunit / cytoplasmic translation / rRNA binding / ribosome / protein ubiquitination / structural constituent of ribosome / ribonucleoprotein complex / translation / positive regulation of protein phosphorylation / mRNA binding / ubiquitin protein ligase binding / nucleolus / RNA binding / zinc ion binding / nucleus / metal ion binding / cytoplasm / cytosol
Similarity search - Function
: / Ribosomal protein S26e signature. / : / Ribosomal protein L41 / Ribosomal protein L41 / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S26e / Ribosomal protein S21e, conserved site / Ribosomal protein S21e signature. ...: / Ribosomal protein S26e signature. / : / Ribosomal protein L41 / Ribosomal protein L41 / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S26e / Ribosomal protein S21e, conserved site / Ribosomal protein S21e signature. / Ribosomal protein S12e signature. / Ribosomal protein S12e / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S19e, conserved site / Ribosomal protein S19e signature. / Small (40S) ribosomal subunit Asc1/RACK1 / Ribosomal protein S2, eukaryotic / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / 40S Ribosomal protein S10 / S27a-like superfamily / Ribosomal protein S10, eukaryotic/archaeal / Plectin/S10, N-terminal / Plectin/S10 domain / Ribosomal protein S25 / S25 ribosomal protein / Ribosomal protein S2, eukaryotic/archaeal / : / Ribosomal protein S17e, conserved site / Ribosomal protein S17e signature. / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein S8e subdomain, eukaryotes / Ribosomal protein S30 / Ribosomal protein S30 / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / Ribosomal protein S7e signature. / Ribosomal protein S3, eukaryotic/archaeal / Ribosomal protein S19e / Ribosomal protein S3Ae, conserved site / Ribosomal protein S19e / Ribosomal protein S3Ae signature. / Ribosomal_S19e / Ribosomal protein S27e signature. / Ribosomal protein S4e, N-terminal, conserved site / Ribosomal protein S4e signature. / 40S ribosomal protein S4, C-terminal domain / 40S ribosomal protein S4 C-terminus / Ribosomal protein S19A/S15e / Ribosomal protein S8e, conserved site / Ribosomal protein S8e signature. / Ribosomal protein S17e / Ribosomal protein S17e-like superfamily / Ribosomal S17 / Ribosomal protein S6, eukaryotic / 40S ribosomal protein S1/3, eukaryotes / Ribosomal protein S4e, N-terminal / RS4NT (NUC023) domain / Ribosomal S24e conserved site / Ribosomal protein S24e signature. / 40S ribosomal protein S11, N-terminal / Ribosomal_S17 N-terminal / Ribosomal protein S7e / Ribosomal protein S7e / Ribosomal protein S4, KOW domain / Ribosomal protein S4e / Ribosomal protein S4e, central region / Ribosomal protein S4e, central domain superfamily / Ribosomal family S4e / Ribosomal protein S27, zinc-binding domain superfamily / Ribosomal protein S24e / Ribosomal protein S23, eukaryotic/archaeal / Ribosomal protein S24e / Ribosomal protein S6/S6e/A/B/2, conserved site / Ribosomal protein S6e signature. / Ribosomal protein S27 / Ribosomal protein S27 / Ribosomal protein S8e / Ribosomal protein S17, archaeal/eukaryotic / Ribosomal protein S3Ae / Ribosomal protein S28e conserved site / Ribosomal S3Ae family / Ribosomal protein S28e signature. / Ribosomal S3Ae family / Ribosomal protein S28e / Ribosomal protein S28e / Ribosomal protein S6e / Ribosomal protein S5/S7, eukaryotic/archaeal / Ribosomal protein S6e / Ribosomal protein S6e / Ribosomal protein S13/S15, N-terminal / Ribosomal protein S15P / Ribosomal S13/S15 N-terminal domain / Ribosomal S13/S15 N-terminal domain / Ribosomal protein S4/S9, eukaryotic/archaeal / Ribosomal protein S8e/ribosomal biogenesis NSA2 / Ribosomal protein S8e / Ribosomal protein S14/S29
Similarity search - Domain/homology
KLLA0B11231p / Small ribosomal subunit protein eS32A / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein eS28 / Ubiquitin-ribosomal protein eS31 fusion protein / KLLA0F25542p / KLLA0F18040p / KLLA0F09812p / KLLA0F07843p / 40S ribosomal protein S12 ...KLLA0B11231p / Small ribosomal subunit protein eS32A / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein eS28 / Ubiquitin-ribosomal protein eS31 fusion protein / KLLA0F25542p / KLLA0F18040p / KLLA0F09812p / KLLA0F07843p / 40S ribosomal protein S12 / Small ribosomal subunit protein eS6 / KLLA0E23673p / 40S ribosomal protein S8 / Small ribosomal subunit protein uS2 / KLLA0E12277p / 40S ribosomal protein S27 / Small ribosomal subunit protein uS14 / KLLA0D10659p / 40S ribosomal protein S3 / 40S ribosomal protein S26 / 40S ribosomal protein S7 / 40S ribosomal protein S24 / 40S ribosomal protein S30 / KLLA0B08173p / KLLA0B07931p / 40S ribosomal protein S25 / Small ribosomal subunit protein eS1 / 40S ribosomal protein S4 / KLLA0B01562p / KLLA0B01474p / KLLA0A10483p / KLLA0A07194p / Small ribosomal subunit protein eS21 / Small ribosomal subunit protein uS9
Similarity search - Component
Biological speciesSaccharomyces cerevisiae S288c (yeast) / Kluyveromyces lactis Saccharomyces cerevisiae S288c (yeast) / Kluyveromyces lactis NRRL Y-1140 (yeast) / Saccharomyces cerevisiae S288C (yeast)
Methodsingle particle reconstruction / cryo EM / Resolution: 4.6 Å
AuthorsDatey A / Khaja FT / Hussain T
Funding support India, 1 items
OrganizationGrant numberCountry
Department of Biotechnology (DBT, India)IA/I/17/2/503313 India
CitationJournal: To Be Published
Title: Yeast eukaryotic initiation factor 4B remodels the mRNA entry site on the small ribosomal subunit
Authors: Datey A / Hussain T
History
DepositionJan 31, 2023-
Header (metadata) releaseFeb 7, 2024-
Map releaseFeb 7, 2024-
UpdateFeb 7, 2024-
Current statusFeb 7, 2024Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_35216.png

Downloads & links

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Map

FileDownload / File: emd_35216.map.gz / Format: CCP4 / Size: 178 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationThis is the cryo-EM map of the 40S-eIF4B complex from yeast.
Voxel sizeX=Y=Z: 1.17 Å
Density
Contour LevelBy AUTHOR: 0.006
Minimum - Maximum-0.022837931 - 0.07513961
Average (Standard dev.)0.00005679381 (±0.0030674885)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions360360360
Spacing360360360
CellA=B=C: 421.19998 Å
α=β=γ: 90.0 °

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Supplemental data

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Additional map: auto-refined map of the 40S-eIF4B complex

Fileemd_35216_additional_1.map
Annotationauto-refined map of the 40S-eIF4B complex
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half-map 2 of the 40S-eIF4B complex

Fileemd_35216_half_map_1.map
AnnotationHalf-map 2 of the 40S-eIF4B complex
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half-map 1 of the 40S-eIF4B complex

Fileemd_35216_half_map_2.map
AnnotationHalf-map 1 of the 40S-eIF4B complex
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : The binary complex of the yeast 40S small ribosomal subunit with ...

EntireName: The binary complex of the yeast 40S small ribosomal subunit with the eukaryotic initiation factor 4B.
Components
  • Complex: The binary complex of the yeast 40S small ribosomal subunit with the eukaryotic initiation factor 4B.
    • Complex: Eukaryotic Initiation Factor 4B (eIF4B).
      • Protein or peptide: 60S ribosomal protein L41-A
    • Complex: yeast 40S small ribosomal subunit
      • RNA: RNA (1780-MER)
      • Protein or peptide: 40S ribosomal protein S0
      • Protein or peptide: 40S ribosomal protein S1
      • Protein or peptide: KLLA0F09812p
      • Protein or peptide: KLLA0D08305p
      • Protein or peptide: 40S ribosomal protein S4
      • Protein or peptide: KLLA0D10659p
      • Protein or peptide: 40S ribosomal protein S6
      • Protein or peptide: 40S ribosomal protein S7
      • Protein or peptide: 40S ribosomal protein S8
      • Protein or peptide: KLLA0E23673p
      • Protein or peptide: KLLA0B08173p
      • Protein or peptide: KLLA0A10483p
      • Protein or peptide: 40S ribosomal protein S12
      • Protein or peptide: KLLA0F18040p
      • Protein or peptide: 40S ribosomal protein S14
      • Protein or peptide: KLLA0F07843p
      • Protein or peptide: 40S ribosomal protein S16
      • Protein or peptide: KLLA0B01474p
      • Protein or peptide: KLLA0B01562p
      • Protein or peptide: KLLA0A07194p
      • Protein or peptide: KLLA0F25542p
      • Protein or peptide: 40S ribosomal protein S21
      • Protein or peptide: KLLA0B07931p
      • Protein or peptide: KLLA0B11231p
      • Protein or peptide: 40S ribosomal protein S24
      • Protein or peptide: 40S ribosomal protein S25
      • Protein or peptide: 40S ribosomal protein S26
      • Protein or peptide: 40S ribosomal protein S27
      • Protein or peptide: 40S ribosomal protein S28
      • Protein or peptide: 40S ribosomal protein S29
      • Protein or peptide: 40S ribosomal protein S30
      • Protein or peptide: Ubiquitin-40S ribosomal protein S27a
      • Protein or peptide: KLLA0E12277p
  • Ligand: MAGNESIUM ION
  • Ligand: ZINC ION

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Supramolecule #1: The binary complex of the yeast 40S small ribosomal subunit with ...

SupramoleculeName: The binary complex of the yeast 40S small ribosomal subunit with the eukaryotic initiation factor 4B.
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#35
Details: 40S purified from Kluyveromyces lactis and Saccharomyces cerevisiae eIF4B overexpressed and purified from Escherichia coli
Source (natural)Organism: Saccharomyces cerevisiae S288c (yeast) / Strain: S288c / Tissue: Single cell yeast / Location in cell: cytoplasm

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Supramolecule #2: Eukaryotic Initiation Factor 4B (eIF4B).

SupramoleculeName: Eukaryotic Initiation Factor 4B (eIF4B). / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #35
Details: eIF4B is a part of the binary complex along with the 40S ribosomal subunit. eIF4B is present and associated with the 40S near its mRNA channel entry site on the solvent-exposed side. The ...Details: eIF4B is a part of the binary complex along with the 40S ribosomal subunit. eIF4B is present and associated with the 40S near its mRNA channel entry site on the solvent-exposed side. The Saccharomyces cerevisiae eIF4B was recombinantly expressed and purified from the E. coli expression system.
Source (natural)Organism: Kluyveromyces lactis Saccharomyces cerevisiae S288c (yeast)
Strain: NRRL Y-1140 / Organ: yeast / Tissue: yeast / Organelle: ribosome / Location in cell: cytoplasm

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Supramolecule #3: yeast 40S small ribosomal subunit

SupramoleculeName: yeast 40S small ribosomal subunit / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #1-#34

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Macromolecule #1: RNA (1780-MER)

MacromoleculeName: RNA (1780-MER) / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast) / Strain: NRRL Y-1140
Molecular weightTheoretical: 579.545875 KDa
SequenceString: UAUCUGGUUG AUCCUGCCAG UAGUCAUAUG CUUGUCUCAA AGAUUAAGCC AUGCAUGUCU AAGUAUAAGC AAUUUAUACA GUGAAACUG CGAAUGGCUC AUUAAAUCAG UUAUCGUUUA UUUGAUAGUU CCUUUACUAC AUGGAUAUCU GUGGUAAUUC U AGAGCUAA ...String:
UAUCUGGUUG AUCCUGCCAG UAGUCAUAUG CUUGUCUCAA AGAUUAAGCC AUGCAUGUCU AAGUAUAAGC AAUUUAUACA GUGAAACUG CGAAUGGCUC AUUAAAUCAG UUAUCGUUUA UUUGAUAGUU CCUUUACUAC AUGGAUAUCU GUGGUAAUUC U AGAGCUAA UACAUGCUUA AAAUCUCGAC CCUUUGGAAG AGAUGUAUUU AUUAGAUAAA AAAUCAAUGU CUUCGGACUC CU UGAUGAU UCAUAAUAAC UUUUCGAAUC GCAUGGCCUU GUGCUGGCGA UGGUUCAUUC AAAUUUCUGC CCUAUCAACU UUC GAUGGU AGGAUAGUGG CCUACCAUGG UUUCAACGGG UAACGGGGAA UAAGGGUUCG AUUCCGGAGA GGGAGCCUGA GAAA CGGCU ACCACAUCCA AGGAAGGCAG CAGGCGCGCA AAUUACCCAA UCCUAAUUCA GGGAGGUAGU GACAAUAAAU AACGA UACA GGGCCCAUUC GGGUCUUGUA AUUGGAAUGA GUACAAUGUA AAUACCUUAA CGAGGAACAA CUGGAGGGCA AGUCUG GUG CCAGCAGCCG CGGUAAUUCC AGCUCCAGUA GCGUAUAUUA AAGUUGUUGC AGUUAAAAAG CUCGUAGUUG AACUUUG GG UCUGGUUGUC CGGUCCGACU UUAUGUCGCG CACUGGUUUU UCAACCGGAU CUUUCCUUCU GGCUAACCUG UACUCCUU G UGGGUGCAGG CGAACCAGGA CUUUUACUUU GAAAAAAUUA GAGUGUUCAA AGCAGGCGAA AGCUCGAAUA UAUUAGCAU GGAAUAAUGG AAUAGGACGU UUGGUUCUAU UUUGUUGGUU UCUAGGACCA UCGUAAUGAU UAAUAGGGAC GGUCGGGGGC AUCAGUAUU CAAUUGUCAG AGGUGAAAUU CUUGGAUUUA UUGAAGACUA ACUACUGCGA AAGCAUUUGC CAAGGACGUU U UCAUUAAU CAAGAACGAA AGUUAGGGGA UCGAAGAUGA UCAGAUACCG UCGUAGUCUU AACCAUAAAC UAUGCCGACU AG GGAUCGG GUGGUGUUUU UCUUAUGACC CACUCGGCAC CUUACGAGAA AUCAAAGUCU UUGGGUUCUG GGGGGAGUAU GGU CGCAAG GCUGAAACUU AAAGGAAUUG ACGGAAGGGC ACCACCAGGA GUGGAGCCUG CGGCUUAAUU UGACUCAACA CGGG GAAAC UCACCAGGUC CAGACACAAU AAGGAUUGAC AGAUUGAGAG CUCUUUCUUG AUUUUGUGGG UGGUGGUGCA UGGCC GUUC UUAGUUGGUG GAGUGAUUUG UCUGCUUAAU UGCGAUAACG AACGAGACCU UAACCUACUA AAUAGGGUUG CUGGCA CUU GCCGGUUGAC UCUUCUUAGA GGGACUAUCG GUUUCAAGCC GAUGGAAGUU UGAGGCAAUA ACAGGUCUGU GAUGCCC UU AGACGUUCUG GGCCGCACGC GCGCUACACU GACGGAGCCA GCGAGUACAA CCUUGGCCGA GAGGUCUGGG UAAUCUUG U GAAACUCCGU CGUGCUGGGG AUAGAGCAUU GUAAUUAUUG CUCUUCAACG AGGAAUUCCU AGUAAGCGCA AGUCAUCAG CUUGCGUUGA UUACGUCCCU GCCCUUUGUA CACACCGCCC GUCGCUAGUA CCGAUUGAAU GGCUUAGUGA GGCCUCAGGA UUUGCUUAG AGAAGGGGGC AACUCCAUCU CAGAGCGAAG AAUCUGGUCA AACUUGGUCA UUUAGAGGAA CUAAAAGUCG U AACAAGGU UUCCGUAGGU GAACCUGCGG AAGGAUCAUU A

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Macromolecule #2: 40S ribosomal protein S0

MacromoleculeName: 40S ribosomal protein S0 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast) / Strain: NRRL Y-1140
Molecular weightTheoretical: 28.264525 KDa
SequenceString: MSLPSTFDLT SEDAQLLLAA RVHLGAKNVQ VHQEPYVYKA RPDGVNVINV GKTWEKIVLA ARIIAAIPNP EDVVAISSRT YGQRAVLKY AAHTGATPIA GRFTPGSFTN YITRSFKEPR LVIVTDPRSD AQAIKESSYV NIPVIALTDL DSPSEYVDVA I PCNNRGKH ...String:
MSLPSTFDLT SEDAQLLLAA RVHLGAKNVQ VHQEPYVYKA RPDGVNVINV GKTWEKIVLA ARIIAAIPNP EDVVAISSRT YGQRAVLKY AAHTGATPIA GRFTPGSFTN YITRSFKEPR LVIVTDPRSD AQAIKESSYV NIPVIALTDL DSPSEYVDVA I PCNNRGKH SIGLIWYLLA REVLRLRGAL PDRTQPWAIM PDLYFYRNPE EIEQQTAEEE AVASGEQTEE AVDATEEQTE AA EWAEEGQ AQEEEWN

UniProtKB: Small ribosomal subunit protein uS2

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Macromolecule #3: 40S ribosomal protein S1

MacromoleculeName: 40S ribosomal protein S1 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast) / Strain: NRRL Y-1140
Molecular weightTheoretical: 28.971643 KDa
SequenceString: MAVGKNKRLS KGKKGLKKRV VDPFTRKEWY DIKAPSTFEN RNVGKTLVNK SVGLKNASDS LKGRVVEVCL ADLQGSEDHS FRKVKLRVD EVQGKNLLTN FHGMDFTTDK LRSMVRKWQT LIEANVTVKT SDDYVLRIFA IAFTRKQANQ VKRTSYAQSS H IRQIRKVI ...String:
MAVGKNKRLS KGKKGLKKRV VDPFTRKEWY DIKAPSTFEN RNVGKTLVNK SVGLKNASDS LKGRVVEVCL ADLQGSEDHS FRKVKLRVD EVQGKNLLTN FHGMDFTTDK LRSMVRKWQT LIEANVTVKT SDDYVLRIFA IAFTRKQANQ VKRTSYAQSS H IRQIRKVI SEILTREVQN STLAQLTSKL IPEVINKEIE NATKDIFPLQ NVHIRKVKLL KQPKFDLGSL LSLHGEASAE EK GKKVAGF KDEILETV

UniProtKB: Small ribosomal subunit protein eS1

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Macromolecule #4: KLLA0F09812p

MacromoleculeName: KLLA0F09812p / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast) / Strain: NRRL Y-1140
Molecular weightTheoretical: 27.649979 KDa
SequenceString: MSAPQAQGQQ APRRGGFGGA NRGGRGGRRG GRRDQEEKGW VPVTKLGRLV KAGKISSIEE IFLHSLPVKE FQIIDQLLPN LKDEVMNIK PVQKQTRAGQ RTRFKAVVVV GDSNGHVGLG IKTAKEVAGA IRAGIIIAKL SVIPIRRGYW GTNLGQPHSL A TKTSGKCG ...String:
MSAPQAQGQQ APRRGGFGGA NRGGRGGRRG GRRDQEEKGW VPVTKLGRLV KAGKISSIEE IFLHSLPVKE FQIIDQLLPN LKDEVMNIK PVQKQTRAGQ RTRFKAVVVV GDSNGHVGLG IKTAKEVAGA IRAGIIIAKL SVIPIRRGYW GTNLGQPHSL A TKTSGKCG SVSVRLIPAP RGSGIVASPA VKKLMQLAGV EDVYTSSTGS TRTLENTLKA AFVAIGNTYG FLTPNLWEVQ AL TPSPMDV YADYATASKK KL

UniProtKB: KLLA0F09812p

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Macromolecule #5: KLLA0D08305p

MacromoleculeName: KLLA0D08305p / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast) / Strain: NRRL Y-1140
Molecular weightTheoretical: 26.300535 KDa
SequenceString: MVAIISKKRK LVADGVFYAE LNEFFTRELA EEGYSGVEVR VTPTKTEIII RATKVQDVVG ENGRRINELT LLIEKRFKYK RGTIALYAE RVHDRGLSAV AQAESMKFKL LNGLAIRRAA YGVVRYVMES GAKGCEVVIS GKLRAARAKS MKFADGFLIH S GQPVNDFI ...String:
MVAIISKKRK LVADGVFYAE LNEFFTRELA EEGYSGVEVR VTPTKTEIII RATKVQDVVG ENGRRINELT LLIEKRFKYK RGTIALYAE RVHDRGLSAV AQAESMKFKL LNGLAIRRAA YGVVRYVMES GAKGCEVVIS GKLRAARAKS MKFADGFLIH S GQPVNDFI ETATRHVLLR QGVLGIKVKI MKDPSRNTSG PKALPDAVTI IEPKEEEPVL EPSVKDYRPT EPVEAAESA

UniProtKB: 40S ribosomal protein S3

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Macromolecule #6: 40S ribosomal protein S4

MacromoleculeName: 40S ribosomal protein S4 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast) / Strain: NRRL Y-1140
Molecular weightTheoretical: 29.617514 KDa
SequenceString: MARGPKKHLK RLAAPHHWML DKLSGCYAPR PSAGPHKLRE SLPLIVFLRN RLKYALNGRE VKAILMQRHV KVDGKVRTDT TFPAGFMDV ITLEATNENF RLVYDVKGRF AVHRITDEEA SYKLAKVKKV QLGKKGIPYV VTHDGRTIRY PDPNIKVNDT V KVDLATGT ...String:
MARGPKKHLK RLAAPHHWML DKLSGCYAPR PSAGPHKLRE SLPLIVFLRN RLKYALNGRE VKAILMQRHV KVDGKVRTDT TFPAGFMDV ITLEATNENF RLVYDVKGRF AVHRITDEEA SYKLAKVKKV QLGKKGIPYV VTHDGRTIRY PDPNIKVNDT V KVDLATGT ITDFIKFDTG KLVYVTGGRN LGRVGTIVHR ERHEGGFDLV HIKDSLENTF VTRLNNVFVI GEPGRPWISL PK GKGIKLT ISEERDRRRA QHGL

UniProtKB: 40S ribosomal protein S4

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Macromolecule #7: KLLA0D10659p

MacromoleculeName: KLLA0D10659p / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast) / Strain: NRRL Y-1140
Molecular weightTheoretical: 25.385975 KDa
SequenceString: MSEHEAQVEV EVQEDFEVVQ EFVPVELATT IPVEIQQAQQ EIKLFNKWSF EDVEVKDASL VDYIQISKPI YVAHTAGRYA NKRFRKAQC PIVERLTNSL MMNGRNNGKK LKAVRIVKHT LEIINVLTDQ NPLQVVVDAI INSGPREDTT RVGGGGAARR Q AVDVSPLR ...String:
MSEHEAQVEV EVQEDFEVVQ EFVPVELATT IPVEIQQAQQ EIKLFNKWSF EDVEVKDASL VDYIQISKPI YVAHTAGRYA NKRFRKAQC PIVERLTNSL MMNGRNNGKK LKAVRIVKHT LEIINVLTDQ NPLQVVVDAI INSGPREDTT RVGGGGAARR Q AVDVSPLR RVNQSIALLT IGAREAAFRN IKTIAETLAE ELINAAKGSS TSYAIKKKDE LERVAKSNR

UniProtKB: KLLA0D10659p

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Macromolecule #8: 40S ribosomal protein S6

MacromoleculeName: 40S ribosomal protein S6 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast) / Strain: NRRL Y-1140
Molecular weightTheoretical: 26.970391 KDa
SequenceString: MKLNISYPIN GTQKCIEIDD EHRVRVFYDK RIGQEVDGES VGDEFKGYVF KIAGGNDKQG FPMKQGVLLP TRVKLLLAKG HSCYRPRRN GERKRKSVRG AIVGPDLAVL ALIITKKGEQ EIEGITNDTV PKRLGPKRAN NIRKFFGLTK EDDVRDYVIR R EVTKGDKS ...String:
MKLNISYPIN GTQKCIEIDD EHRVRVFYDK RIGQEVDGES VGDEFKGYVF KIAGGNDKQG FPMKQGVLLP TRVKLLLAKG HSCYRPRRN GERKRKSVRG AIVGPDLAVL ALIITKKGEQ EIEGITNDTV PKRLGPKRAN NIRKFFGLTK EDDVRDYVIR R EVTKGDKS YTKAPKIQRL VTPQRLQRKR QQKSLKIKNA QAQREAAAEY AQLLAKRLSE RKAEKAEVRK RRASSLKA

UniProtKB: Small ribosomal subunit protein eS6

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Macromolecule #9: 40S ribosomal protein S7

MacromoleculeName: 40S ribosomal protein S7 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast) / Strain: NRRL Y-1140
Molecular weightTheoretical: 21.735297 KDa
SequenceString:
MSDPQAKILS QAPTELELQV AQAFIDLENN SPELKADLRA LQFKSIREIE VAGGKKALAV FVPVPSLAAY HKVQIKLTRE LEKKFQDRH VIFLAERRIL PKPSRKSRQT QKRPRSRTLT AVHDKILEDL VFPTEIVGKR VRYLVGGNKI QKILLNSKDV Q HIDNKLES FQAVYNKLTG KQIVFEIPSE TH

UniProtKB: 40S ribosomal protein S7

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Macromolecule #10: 40S ribosomal protein S8

MacromoleculeName: 40S ribosomal protein S8 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast) / Strain: NRRL Y-1140
Molecular weightTheoretical: 22.642727 KDa
SequenceString: MGISRDSRHK RAATGAKRAQ FRKKRKFELG RQAANTKIGT KRIHPVRTRG GNQKFRALRI ETGNFSWASE GVARKTRITG VVYHPSNNE LVRTNTLTKA AIVQIDATPF RQWYESHYGQ SLGKKKNTKA EEETATTSKN TERKWAARAA EAKIEHAVDS Q FGAGRLYA ...String:
MGISRDSRHK RAATGAKRAQ FRKKRKFELG RQAANTKIGT KRIHPVRTRG GNQKFRALRI ETGNFSWASE GVARKTRITG VVYHPSNNE LVRTNTLTKA AIVQIDATPF RQWYESHYGQ SLGKKKNTKA EEETATTSKN TERKWAARAA EAKIEHAVDS Q FGAGRLYA AISSRPGQSG RCDGYILEGE ELAFYLRRLT AKK

UniProtKB: 40S ribosomal protein S8

+
Macromolecule #11: KLLA0E23673p

MacromoleculeName: KLLA0E23673p / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast) / Strain: NRRL Y-1140
Molecular weightTheoretical: 21.587049 KDa
SequenceString:
MPRAPRTYSK TYSTPKRPYE SARLDAELKL AGEYGLKNKR EIYRISFQLS KIRRAARDLL TRDEKDPKRL FEGNALIRRL VRIGVLSED KKKLDYVLAL KVEDFLERRL QTQVYKLGLA KSVHHARVLI SQRHIAVGKQ IVNIPSFMVR LESEKHIDFA R TSPFGGAR PGRVARKRAA AAGGEEADEE

UniProtKB: KLLA0E23673p

+
Macromolecule #12: KLLA0B08173p

MacromoleculeName: KLLA0B08173p / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast) / Strain: NRRL Y-1140
Molecular weightTheoretical: 12.584377 KDa
SequenceString:
MLIPKEDRKK IYQHLFQEGV LVAKKDFNQP KHEEIDTKNL FVIKALQSLT SKGFVKTQFS WQYYYYTLTE EGVVYLREYL NLPEHIFPA TYLAGQSGDQ RPQGKKY

UniProtKB: KLLA0B08173p

+
Macromolecule #13: KLLA0A10483p

MacromoleculeName: KLLA0A10483p / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast) / Strain: NRRL Y-1140
Molecular weightTheoretical: 17.84393 KDa
SequenceString:
MSTELTVQSE RAFQKQPHIF TNPKAKANRK TKRWYKNVGL GFKTPKTAIE GSYIDKKCPF TGLVSIRGKI LTGTVVSTRM HRTIVIRRD YLHYVPKYNR YEKRHKNVPA HVSPAFRVQV GDIVTVGQCR PISKTVRFNV LKVASATGKA NKQFAKF

UniProtKB: KLLA0A10483p

+
Macromolecule #14: 40S ribosomal protein S12

MacromoleculeName: 40S ribosomal protein S12 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast) / Strain: NRRL Y-1140
Molecular weightTheoretical: 14.466398 KDa
SequenceString:
MSDVEEVQQV PVAELTIEDA LKVVLRTSLV HDGLARGLRE SAKALTRGEG QLAVLVESVT EEAISKLVQG LATENNVPLI KVADAKQLG EWAGLGKIDR DGNARKVVGA SVVVVKNWGA DTQEREILLE HFSQQ

UniProtKB: 40S ribosomal protein S12

+
Macromolecule #15: KLLA0F18040p

MacromoleculeName: KLLA0F18040p / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast) / Strain: NRRL Y-1140
Molecular weightTheoretical: 16.989875 KDa
SequenceString:
MGRMHSKGKG MSSSAIPYSR NAPAWFKGSS DGVVEQIIKY ARKGLTPSQI GVLLRDAHGV TQAKVITGNK ILRILKSNGL APEIPEDLY FLIKKAVSVR KHLERNRKDK DAKFRLILIE SRIHRLARYY RTVSVLPPNW KYESATASAL VN

UniProtKB: KLLA0F18040p

+
Macromolecule #16: 40S ribosomal protein S14

MacromoleculeName: 40S ribosomal protein S14 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast) / Strain: NRRL Y-1140
Molecular weightTheoretical: 14.530655 KDa
SequenceString:
MANVVQAKDN SQVFGVARIF ASFNDTFVHV TDLSGRETIA RVTGGMKVKA DRDESSPYAA MLAAQDVAAK CKEVGITAVH IKIRATGGT RSKTPGPGGQ AALRALARSG LRIGRIEDVT PVPSDSTRKK GGRRGRRL

UniProtKB: Small ribosomal subunit protein uS11

+
Macromolecule #17: KLLA0F07843p

MacromoleculeName: KLLA0F07843p / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast) / Strain: NRRL Y-1140
Molecular weightTheoretical: 15.986796 KDa
SequenceString:
MSEAAAPRKR SFKTYSYKGV DLEKLLEMPT EDFVKLAPAR VRRKFARGLS EKPAGLMKKL RAAKLSAPEN EKPAVVRTHL RNMIIVPEM IGSVVGVYNG KVFNQVEIRP EMVGHYLGEF SITYTPVRHG RAGATTSRFI PLR

UniProtKB: KLLA0F07843p

+
Macromolecule #18: 40S ribosomal protein S16

MacromoleculeName: 40S ribosomal protein S16 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast) / Strain: NRRL Y-1140
Molecular weightTheoretical: 15.874531 KDa
SequenceString:
MSTVPSVQTF GKKKSATAVA HVKAGKGLIK VNGSPITLVQ PEILRFKVYE PLLLVGLDKF ANIDIRVKVT GGGHVSQVYA IRQAIAKGL VAYHQKFVDE QSKNELKKAF TSYDRTLLIA DSRRPEPKKF GGRGARSRFQ KSYR

UniProtKB: Small ribosomal subunit protein uS9

+
Macromolecule #19: KLLA0B01474p

MacromoleculeName: KLLA0B01474p / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast) / Strain: NRRL Y-1140
Molecular weightTheoretical: 15.722216 KDa
SequenceString:
MGRVRTKTVK RASKALIEKY YPKLTMDFQT NKRLCDEIAT IQSKRLRNKI AGYTTHLMKR IQKGPVRGIS FKLQEEERER KDQYVPDVS ALDLSHSNDV LNVDTQTAEL VNSLGLKLPL SVSSVSAVRD RRFRKRN

UniProtKB: KLLA0B01474p

+
Macromolecule #20: KLLA0B01562p

MacromoleculeName: KLLA0B01562p / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast) / Strain: NRRL Y-1140
Molecular weightTheoretical: 17.084602 KDa
SequenceString:
MSLVVQEQGS FQHILRLLNT NVDGNINVVY ALTTIRGVGR RYANLVCKKA DVDLHKRAGE LTQEELERIV QIMQNPTHYK IPAWFLNRQ KDVNDGKDYH SLANNLESKL RDDLERLKKI RSHRGIRHFW GLRVRGQHTK TTGRRRA

UniProtKB: KLLA0B01562p

+
Macromolecule #21: KLLA0A07194p

MacromoleculeName: KLLA0A07194p / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast) / Strain: NRRL Y-1140
Molecular weightTheoretical: 15.87901 KDa
SequenceString:
MPGVSVRDVP AQDFINNYAS FLQRQGKLEV PGYVDIVKTS AGNELPPQDS EGWFYKRAAS VARHIYLRKQ VGVGKLNKLY GGAKNRGVR PHKHVDASGS INRKVLQSLE KLGVVEISPK GGRRISDNGL RDLDRIAAAT LEDEE

UniProtKB: KLLA0A07194p

+
Macromolecule #22: KLLA0F25542p

MacromoleculeName: KLLA0F25542p / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast) / Strain: NRRL Y-1140
Molecular weightTheoretical: 13.337604 KDa
SequenceString:
MSQVEKKSEQ QQEVVIHKIR INLTSTKVKQ LENVSANIIK NAETFKLVKK GPVRLPTKVL KISTRKTPNG EGSKTWDTYE MRIHKRYID LEAPAHIVKR ITQITIEPGV DVEVIIAA

UniProtKB: KLLA0F25542p

+
Macromolecule #23: 40S ribosomal protein S21

MacromoleculeName: 40S ribosomal protein S21 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast) / Strain: NRRL Y-1140
Molecular weightTheoretical: 9.797949 KDa
SequenceString:
MENDKGQLVE LYVPRKCSAT NRIIKAKDHS SVQINIAQVD EEGRAIPGEY VTYALSGYIR ARGEADDSLN RLAQQDGLLK NVWSYSR

UniProtKB: Small ribosomal subunit protein eS21

+
Macromolecule #24: KLLA0B07931p

MacromoleculeName: KLLA0B07931p / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast) / Strain: NRRL Y-1140
Molecular weightTheoretical: 14.645041 KDa
SequenceString:
MTRTSVLADA LNAINNAEKT GKRQVLIRPS SKVIIKFLQV MQKHGYIGEF EYIDDHRSGK IVVQLNGRLN KCGVISPRFN VKIADVEKW TANLLPARQF GYVILTTSAG IMDHEEAHRK HVSGKILGFV Y

UniProtKB: KLLA0B07931p

+
Macromolecule #25: KLLA0B11231p

MacromoleculeName: KLLA0B11231p / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast) / Strain: NRRL Y-1140
Molecular weightTheoretical: 16.047897 KDa
SequenceString:
MGKGKPRGLN SARKLRVHRR NNRWAETTYK KRLLGTAFKS SPFGGSSHAK GIVLEKIGIE SKQPNSAIRK CVRVQLIKNG KKVTAFVPN DGCLNFVDEN DEVLLAGFGR KGKAKGDIPG VRFKVVKVSG VSLLALWKEK KEKPRS

UniProtKB: KLLA0B11231p

+
Macromolecule #26: 40S ribosomal protein S24

MacromoleculeName: 40S ribosomal protein S24 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast) / Strain: NRRL Y-1140
Molecular weightTheoretical: 15.194549 KDa
SequenceString:
MSDAITIRTR KVISNPLLAR KQFVVDVLHP NRANVSKDEL REKLAEAYKA EKDAVSVFGF RTQYGGGKST GFGLVYNSVA DAKKFEPAY RLVRYGLAEK VEKASRQQRK QRKNRGKKIF GTGKSIAKKA ARRNAD

UniProtKB: 40S ribosomal protein S24

+
Macromolecule #27: 40S ribosomal protein S25

MacromoleculeName: 40S ribosomal protein S25 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast) / Strain: NRRL Y-1140
Molecular weightTheoretical: 12.002116 KDa
SequenceString:
MPPKQQLSKA AKAAAAMAGG KKSKKKWSKK SHKDKAKHAV VLDQDKFDRI MKEAPTYRYV SVSVLVDRFK LGGSLARVAL RHLENEGII KPVSKHSKQA IYTRATASE

UniProtKB: 40S ribosomal protein S25

+
Macromolecule #28: 40S ribosomal protein S26

MacromoleculeName: 40S ribosomal protein S26 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast) / Strain: NRRL Y-1140
Molecular weightTheoretical: 13.539957 KDa
SequenceString:
MPKKRASNGR NKKGRGHVKP VRCVNCSRSV PKDKAIKRMA IRNIVEAAAI RDLSEASVYA EYALPKTYNK LHYCISCAIH ARIVRVRSR TDRRIRAPPQ RPRFNRDNKV SPADAAKKAL

UniProtKB: 40S ribosomal protein S26

+
Macromolecule #29: 40S ribosomal protein S27

MacromoleculeName: 40S ribosomal protein S27 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast) / Strain: NRRL Y-1140
Molecular weightTheoretical: 8.884362 KDa
SequenceString:
MVLVQDLLHP TAASEARKHK LKTLVQSPRS HFLDVKCPGC LNITTVFSHA QTAVTCESCS TVLCTPTGGK AKLSEGTSFR RK

UniProtKB: 40S ribosomal protein S27

+
Macromolecule #30: 40S ribosomal protein S28

MacromoleculeName: 40S ribosomal protein S28 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast) / Strain: NRRL Y-1140
Molecular weightTheoretical: 7.549824 KDa
SequenceString:
MDTKTPVTLA KVIKVLGRTG SRGGVTQVRV EFLEDTTRTI VRNVKGPVRE GDILVLMESE REARRLR

UniProtKB: Small ribosomal subunit protein eS28

+
Macromolecule #31: 40S ribosomal protein S29

MacromoleculeName: 40S ribosomal protein S29 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast) / Strain: NRRL Y-1140
Molecular weightTheoretical: 6.66257 KDa
SequenceString:
MAHENVWYSH PRKFGKGSRQ CRISGSHSGL IRKYGLNIDR QSFREKANDI GFYKYR

UniProtKB: Small ribosomal subunit protein uS14

+
Macromolecule #32: 40S ribosomal protein S30

MacromoleculeName: 40S ribosomal protein S30 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast) / Strain: NRRL Y-1140
Molecular weightTheoretical: 7.141421 KDa
SequenceString:
MGKVHGSLAR AGKVKSQTPK VEKQEKPKQP KGRAYKRLLY TRRFVNVTLT NGKRKMNPSP SSQ

UniProtKB: 40S ribosomal protein S30

+
Macromolecule #33: Ubiquitin-40S ribosomal protein S27a

MacromoleculeName: Ubiquitin-40S ribosomal protein S27a / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast) / Strain: NRRL Y-1140
Molecular weightTheoretical: 17.110977 KDa
SequenceString:
MQIFVKTLTG KTITLEVESS DTIDNVKSKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGGGKKR KKKVYTTPK KIRHKHKKVK LAVLNYYKVD DEGKVAKLRK ECPNCGPGIF LANHGDRFYC GKCHSTFATQ K

UniProtKB: Ubiquitin-ribosomal protein eS31 fusion protein

+
Macromolecule #34: KLLA0E12277p

MacromoleculeName: KLLA0E12277p / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Kluyveromyces lactis NRRL Y-1140 (yeast) / Strain: NRRL Y-1140
Molecular weightTheoretical: 35.830945 KDa
SequenceString: MSSSNIMLVL RGTLEGHNGW VTSLSTSAAQ PNLLVSGSRD KTLISWRLTE NEQQFGVPVR SYKGHSHIVQ DVVVSADGNY AVSASWDKT LRLWNLATGN SEARFVGHTG DVLSVAIDAN SSKIISASRD KTIRVWNTVG DCAYVLLGHT DWVTKVRVAP K NLEDGEVD ...String:
MSSSNIMLVL RGTLEGHNGW VTSLSTSAAQ PNLLVSGSRD KTLISWRLTE NEQQFGVPVR SYKGHSHIVQ DVVVSADGNY AVSASWDKT LRLWNLATGN SEARFVGHTG DVLSVAIDAN SSKIISASRD KTIRVWNTVG DCAYVLLGHT DWVTKVRVAP K NLEDGEVD DGRITFVSAG MDKIVRSWSL NEDSYRIEAD FIGHNNYINV VQPSPDGSLA ASAGKDGQIY VWNLKHKSAF MN FDAKDEV FALAFSPSRF WLTAATASGI KIYDLENEVL IDELKPEFAG YTKAQDPHAV SLAWSADGQT LFAGYTDNVI RVW QVMTAN

UniProtKB: KLLA0E12277p

+
Macromolecule #35: 60S ribosomal protein L41-A

MacromoleculeName: 60S ribosomal protein L41-A / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast) / Strain: S288c
Molecular weightTheoretical: 3.354243 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString:
MRAKWRKKRT RRLKRKRRKV RARSK

UniProtKB: Small ribosomal subunit protein eS32A

+
Macromolecule #36: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 36 / Number of copies: 2 / Formula: MG
Molecular weightTheoretical: 24.305 Da

+
Macromolecule #37: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 37 / Number of copies: 3 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

BufferpH: 7.4
Component:
ConcentrationFormulaName
20.0 mMC8H18N2O4SHEPES
100.0 mMCH3CO2KPotassium acetate
2.5 mMC2H6O4MgMagnesium acetate
2.0 mMC4H10O2S2Dithiothreitol

Details: 20 mM HEPES pH7.4, 100 mM potassium acetate pH7.6, 2.5 mM Magnesium acetate, 2 mM DTT
GridModel: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 90 sec. / Pretreatment - Atmosphere: AIR / Pretreatment - Pressure: 100.0 kPa
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 295 K / Instrument: FEI VITROBOT MARK IV
Details: Vitrification was carried out in nitrogen atmosphere..
DetailsThis class of empty or only 40S was a part of the reaction mixture containing 40S and the Saccharomyces cerevisiae eukaryotic initiation factor 4B present in a 1:10 molar ratio

-
Electron microscopy

MicroscopeFEI TALOS ARCTICA
TemperatureMin: 77.0 K / Max: 98.0 K
DetailsPreliminary grid screening was done manually using the Latitude-S
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Digitization - Frames/image: 4-16 / Number grids imaged: 6 / Number real images: 7975 / Average exposure time: 8.0 sec. / Average electron dose: 50.0 e/Å2
Details: Images were collected in movie-mode at 20 frames in 8 seconds
Electron beamAcceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 70.0 µm / Calibrated defocus max: 3.0 µm / Calibrated defocus min: 1.5 µm / Calibrated magnification: 42000 / Illumination mode: OTHER / Imaging mode: DIFFRACTION / Cs: 2.7 mm / Nominal defocus max: 3.0 µm / Nominal defocus min: 1.5 µm / Nominal magnification: 42000
Sample stageCooling holder cryogen: NITROGEN
Experimental equipment
Model: Talos Arctica / Image courtesy: FEI Company

+
Image processing

Particle selectionNumber selected: 1492547
Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

3jam


Details: map generated from 3JAM was low-pass filtered and used as a reference for 3D autorefinement
Final reconstructionNumber classes used: 1 / Applied symmetry - Point group: C1 (asymmetric) / Algorithm: BACK PROJECTION / Resolution.type: BY AUTHOR / Resolution: 4.6 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.1.1) / Number images used: 108616
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.1.1)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.1.1)
Final 3D classificationNumber classes: 2
FSC plot (resolution estimation)

-
Atomic model buiding 1

RefinementProtocol: RIGID BODY FIT / Target criteria: Cross-correlation coefficient
Output model

PDB-8i7j:
Yeast 40S-eIF4B - partially open conformation of the 40S head

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