- EMDB-35010: Human Consensus Olfactory Receptor OR52c in Complex with Octanoic... -
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基本情報
登録情報
データベース: EMDB / ID: EMD-35010
タイトル
Human Consensus Olfactory Receptor OR52c in Complex with Octanoic Acid (OCA) and G Protein
マップデータ
This is a map of Human consensus olfactory receptor OR52c in complex with octanoic acid and G protein. It is a merged map of receptor-focused and G protein-focused local refinement maps.
試料
複合体: Complex of Consensus Olfactory receptor OR52c in complex with Octanoic acid, G protein and Nanobody 35 (Nb35)
タンパク質・ペプチド: Guanine nucleotide-binding protein G(s) subunit alpha isoforms short
タンパク質・ペプチド: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
タンパク質・ペプチド: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2
タンパク質・ペプチド: Nanobody 35
タンパク質・ペプチド: Consensus Olfactory Receptor OR52c
リガンド: OCTANOIC ACID (CAPRYLIC ACID)
キーワード
Olfactory Receptor / G Protein / MEMBRANE PROTEIN / GPCR / Olfactory GPCR
機能・相同性
機能・相同性情報
PKA activation in glucagon signalling / hair follicle placode formation / mu-type opioid receptor binding / developmental growth / corticotropin-releasing hormone receptor 1 binding / intracellular transport / D1 dopamine receptor binding / Hedgehog 'off' state / beta-2 adrenergic receptor binding / adenylate cyclase-activating adrenergic receptor signaling pathway ...PKA activation in glucagon signalling / hair follicle placode formation / mu-type opioid receptor binding / developmental growth / corticotropin-releasing hormone receptor 1 binding / intracellular transport / D1 dopamine receptor binding / Hedgehog 'off' state / beta-2 adrenergic receptor binding / adenylate cyclase-activating adrenergic receptor signaling pathway / activation of adenylate cyclase activity / adenylate cyclase activator activity / trans-Golgi network membrane / insulin-like growth factor receptor binding / ionotropic glutamate receptor binding / G-protein beta/gamma-subunit complex binding / bone development / Olfactory Signaling Pathway / Activation of the phototransduction cascade / G beta:gamma signalling through PLC beta / Presynaptic function of Kainate receptors / Thromboxane signalling through TP receptor / adenylate cyclase-activating G protein-coupled receptor signaling pathway / G-protein activation / G protein-coupled acetylcholine receptor signaling pathway / Activation of G protein gated Potassium channels / Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits / Prostacyclin signalling through prostacyclin receptor / Glucagon signaling in metabolic regulation / G beta:gamma signalling through CDC42 / cognition / ADP signalling through P2Y purinoceptor 12 / G beta:gamma signalling through BTK / Synthesis, secretion, and inactivation of Glucagon-like Peptide-1 (GLP-1) / Sensory perception of sweet, bitter, and umami (glutamate) taste / photoreceptor disc membrane / platelet aggregation / Adrenaline,noradrenaline inhibits insulin secretion / Glucagon-type ligand receptors / Vasopressin regulates renal water homeostasis via Aquaporins / G alpha (z) signalling events / cellular response to catecholamine stimulus / Glucagon-like Peptide-1 (GLP1) regulates insulin secretion / ADORA2B mediated anti-inflammatory cytokines production / sensory perception of taste / ADP signalling through P2Y purinoceptor 1 / adenylate cyclase-activating dopamine receptor signaling pathway / G beta:gamma signalling through PI3Kgamma / cellular response to prostaglandin E stimulus / Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding / GPER1 signaling / G-protein beta-subunit binding / Inactivation, recovery and regulation of the phototransduction cascade / heterotrimeric G-protein complex / G alpha (12/13) signalling events / extracellular vesicle / sensory perception of smell / signaling receptor complex adaptor activity / Thrombin signalling through proteinase activated receptors (PARs) / GTPase binding / retina development in camera-type eye / phospholipase C-activating G protein-coupled receptor signaling pathway / Ca2+ pathway / positive regulation of cold-induced thermogenesis / G alpha (i) signalling events / fibroblast proliferation / G alpha (s) signalling events / G alpha (q) signalling events / cell population proliferation / Ras protein signal transduction / Extra-nuclear estrogen signaling / G protein-coupled receptor signaling pathway / lysosomal membrane / GTPase activity / synapse / protein-containing complex binding / GTP binding / signal transduction / extracellular exosome / membrane / metal ion binding / plasma membrane / cytoplasm / cytosol 類似検索 - 分子機能
ジャーナル: Nat Commun / 年: 2023 タイトル: Understanding the molecular mechanisms of odorant binding and activation of the human OR52 family. 著者: Chulwon Choi / Jungnam Bae / Seonghan Kim / Seho Lee / Hyunook Kang / Jinuk Kim / Injin Bang / Kiheon Kim / Won-Ki Huh / Chaok Seok / Hahnbeom Park / Wonpil Im / Hee-Jung Choi / 要旨: Structural and mechanistic studies on human odorant receptors (ORs), key in olfactory signaling, are challenging because of their low surface expression in heterologous cells. The recent structure of ...Structural and mechanistic studies on human odorant receptors (ORs), key in olfactory signaling, are challenging because of their low surface expression in heterologous cells. The recent structure of OR51E2 bound to propionate provided molecular insight into odorant recognition, but the lack of an inactive OR structure limited understanding of the activation mechanism of ORs upon odorant binding. Here, we determined the cryo-electron microscopy structures of consensus OR52 (OR52), a representative of the OR52 family, in the ligand-free (apo) and octanoate-bound states. The apo structure of OR52 reveals a large opening between transmembrane helices (TMs) 5 and 6. A comparison between the apo and active structures of OR52 demonstrates the inward and outward movements of the extracellular and intracellular segments of TM6, respectively. These results, combined with molecular dynamics simulations and signaling assays, shed light on the molecular mechanisms of odorant binding and activation of the OR52 family.
ダウンロード / ファイル: emd_35010.map.gz / 形式: CCP4 / 大きさ: 244.1 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈
This is a map of Human consensus olfactory receptor OR52c in complex with octanoic acid and G protein. It is a merged map of receptor-focused and G protein-focused local refinement maps.
ボクセルのサイズ
X=Y=Z: 0.851 Å
密度
表面レベル
登録者による: 0.7
最小 - 最大
-2.7104404 - 5.018383
平均 (標準偏差)
0.024314273 (±0.079772666)
対称性
空間群: 1
詳細
EMDB XML:
マップ形状
Axis order
X
Y
Z
Origin
0
0
0
サイズ
400
400
400
Spacing
400
400
400
セル
A=B=C: 340.4 Å α=β=γ: 90.0 °
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添付データ
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追加マップ: This is a map of Human consensus olfactory...
This is a map of Human consensus olfactory receptor OR52c in complex with octanoic acid and G protein. It is a receptor-focused local refinement sharp map.
This is a map of Human consensus olfactory receptor OR52c in complex with octanoic acid and G protein. It is a receptor-focused local refinement sharp map.
全体 : Complex of Consensus Olfactory receptor OR52c in complex with Oct...
全体
名称: Complex of Consensus Olfactory receptor OR52c in complex with Octanoic acid, G protein and Nanobody 35 (Nb35)
要素
複合体: Complex of Consensus Olfactory receptor OR52c in complex with Octanoic acid, G protein and Nanobody 35 (Nb35)
タンパク質・ペプチド: Guanine nucleotide-binding protein G(s) subunit alpha isoforms short
タンパク質・ペプチド: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
タンパク質・ペプチド: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2
タンパク質・ペプチド: Nanobody 35
タンパク質・ペプチド: Consensus Olfactory Receptor OR52c
リガンド: OCTANOIC ACID (CAPRYLIC ACID)
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超分子 #1: Complex of Consensus Olfactory receptor OR52c in complex with Oct...
超分子
名称: Complex of Consensus Olfactory receptor OR52c in complex with Octanoic acid, G protein and Nanobody 35 (Nb35) タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: #1-#5
由来(天然)
生物種: Homo sapiens (ヒト)
分子量
理論値: 140 KDa
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分子 #1: Guanine nucleotide-binding protein G(s) subunit alpha isoforms short
分子
名称: Guanine nucleotide-binding protein G(s) subunit alpha isoforms short タイプ: protein_or_peptide / ID: 1 / コピー数: 1 / 光学異性体: LEVO