Journal: Nat Commun / Year: 2023 Title: Structural basis for a conserved neutralization epitope on the receptor-binding domain of SARS-CoV-2. Authors: Kuan-Ying A Huang / Xiaorui Chen / Arpita Mohapatra / Hong Thuy Vy Nguyen / Lisa Schimanski / Tiong Kit Tan / Pramila Rijal / Susan K Vester / Rory A Hills / Mark Howarth / Jennifer R Keeffe ...Authors: Kuan-Ying A Huang / Xiaorui Chen / Arpita Mohapatra / Hong Thuy Vy Nguyen / Lisa Schimanski / Tiong Kit Tan / Pramila Rijal / Susan K Vester / Rory A Hills / Mark Howarth / Jennifer R Keeffe / Alexander A Cohen / Leesa M Kakutani / Yi-Min Wu / Md Shahed-Al-Mahmud / Yu-Chi Chou / Pamela J Bjorkman / Alain R Townsend / Che Ma / Abstract: Antibody-mediated immunity plays a crucial role in protection against SARS-CoV-2 infection. We isolated a panel of neutralizing anti-receptor-binding domain (RBD) antibodies elicited upon natural ...Antibody-mediated immunity plays a crucial role in protection against SARS-CoV-2 infection. We isolated a panel of neutralizing anti-receptor-binding domain (RBD) antibodies elicited upon natural infection and vaccination and showed that they recognize an immunogenic patch on the internal surface of the core RBD, which faces inwards and is hidden in the "down" state. These antibodies broadly neutralize wild type (Wuhan-Hu-1) SARS-CoV-2, Beta and Delta variants and some are effective against other sarbecoviruses. We observed a continuum of partially overlapping antibody epitopes from lower to upper part of the inner face of the RBD and some antibodies extend towards the receptor-binding motif. The majority of antibodies are substantially compromised by three mutational hotspots (S371L/F, S373P and S375F) in the lower part of the Omicron BA.1, BA.2 and BA.4/5 RBD. By contrast, antibody IY-2A induces a partial unfolding of this variable region and interacts with a conserved conformational epitope to tolerate all antigenic variations and neutralize diverse sarbecoviruses as well. This finding establishes that antibody recognition is not limited to the normal surface structures on the RBD. In conclusion, the delineation of functionally and structurally conserved RBD epitopes highlights potential vaccine and therapeutic candidates for COVID-19.
Entire : Complex of Delta Spike and IY-2A with two Fabs bound to one Spike...
Entire
Name: Complex of Delta Spike and IY-2A with two Fabs bound to one Spike in 3-RBD-up conformation
Components
Complex: Complex of Delta Spike and IY-2A with two Fabs bound to one Spike in 3-RBD-up conformation
Complex: SARS-CoV-2 Delta Spike protein
Protein or peptide: Spike glycoprotein
Complex: IY-2A
Protein or peptide: IY-2A Fab heavy chain
Protein or peptide: IY-2A Fab light chain
-
Supramolecule #1: Complex of Delta Spike and IY-2A with two Fabs bound to one Spike...
Supramolecule
Name: Complex of Delta Spike and IY-2A with two Fabs bound to one Spike in 3-RBD-up conformation type: complex / ID: 1 / Chimera: Yes / Parent: 0 / Macromolecule list: all
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