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- EMDB-32325: Cryo-EM structure of Tetrahymena thermophila mitochondrial comple... -

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Basic information

Entry
Database: EMDB / ID: EMD-32325
TitleCryo-EM structure of Tetrahymena thermophila mitochondrial complex IV, composite dimer model
Map dataTetrahymena thermophila electron transport chain complex IV dimer composite map. Combined by Phenix from 3.02 angstrom protomer map focus-refined in cryosparc.
Sample
  • Complex: Cytochrome c oxidase dimer
    • Protein or peptide: x 58 types
  • Ligand: x 7 types
Function / homology
Function and homology information


thiosulfate transmembrane transporter activity / oxaloacetate transmembrane transporter activity / malate transmembrane transporter activity / protein maturation by [2Fe-2S] cluster transfer / succinate transmembrane transporter activity / sulfate transmembrane transporter activity / mitochondrial cytochrome c oxidase assembly / phosphate ion transmembrane transport / : / cytochrome-c oxidase ...thiosulfate transmembrane transporter activity / oxaloacetate transmembrane transporter activity / malate transmembrane transporter activity / protein maturation by [2Fe-2S] cluster transfer / succinate transmembrane transporter activity / sulfate transmembrane transporter activity / mitochondrial cytochrome c oxidase assembly / phosphate ion transmembrane transport / : / cytochrome-c oxidase / antiporter activity / mitochondrial electron transport, cytochrome c to oxygen / cytochrome-c oxidase activity / Golgi organization / transmembrane transporter activity / endoplasmic reticulum-Golgi intermediate compartment / electron transport coupled proton transport / ATP synthesis coupled electron transport / 2 iron, 2 sulfur cluster binding / protein transport / monoatomic ion channel activity / nucleic acid binding / mitochondrial inner membrane / membrane => GO:0016020 / copper ion binding / DNA-binding transcription factor activity / heme binding / endoplasmic reticulum / mitochondrion / zinc ion binding / membrane / nucleus / metal ion binding
Similarity search - Function
Protein of unknown function DUF4441 / Domain of unknown function (DUF4441) / Surfeit locus 1/Shy1 / Surfeit locus 1/4 / TraB/PryY-like / SURF1 family / SURF1 family profile. / CTF transcription factor/nuclear factor 1, DNA-binding domain / CTF/NF-I DNA-binding domain profile. / Tim10-like ...Protein of unknown function DUF4441 / Domain of unknown function (DUF4441) / Surfeit locus 1/Shy1 / Surfeit locus 1/4 / TraB/PryY-like / SURF1 family / SURF1 family profile. / CTF transcription factor/nuclear factor 1, DNA-binding domain / CTF/NF-I DNA-binding domain profile. / Tim10-like / Tim10-like domain superfamily / Tim10/DDP family zinc finger / Mitochondrial substrate/solute carrier / Mitochondrial carrier domain superfamily / Mitochondrial carrier protein / Solute carrier (Solcar) repeat profile. / Iron-binding zinc finger, CDGSH type / MitoNEET, CDGSH iron-sulfur domain / CDGSH-type zinc finger. Function unknown. / Regulator of chromosome condensation (RCC1) repeat / Regulator of chromosome condensation, RCC1 / Regulator of chromosome condensation (RCC1) repeat profile. / Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II / Translocon Sec61/SecY, plug domain / Plug domain of Sec61p / Cytochrome c oxidase subunit Vb, zinc binding region signature. / Protein secY signature 1. / Protein secY signature 2. / Cytochrome c oxidase, subunit Vb / Cytochrome c oxidase, subunit Vb superfamily / Cytochrome c oxidase subunit Vb / Cytochrome c oxidase subunit Vb, zinc binding domain profile. / SecY/SEC61-alpha family / SecY domain superfamily / SecY conserved site / SecY / Cytochrome c/quinol oxidase subunit II / Cytochrome c oxidase, subunit I, copper-binding site / Heme-copper oxidase catalytic subunit, copper B binding region signature. / Cytochrome c oxidase-like, subunit I domain / Cytochrome oxidase subunit I profile. / Cytochrome c oxidase subunit I / Cytochrome c oxidase-like, subunit I superfamily / Cytochrome C and Quinol oxidase polypeptide I / Cytochrome C oxidase subunit II, periplasmic domain / Cytochrome c oxidase subunit II-like C-terminal / Cytochrome oxidase subunit II copper A binding domain profile. / Pectin lyase fold/virulence factor / Cyclic nucleotide-monophosphate binding domain / Cyclic nucleotide-binding domain / cAMP/cGMP binding motif profile. / Cyclic nucleotide-binding domain / Cyclic nucleotide-binding domain superfamily / RmlC-like jelly roll fold / Cupredoxin / Ion transport domain / Ion transport protein / zinc finger / Zinc knuckle / Zinc finger, CCHC-type superfamily / Zinc finger, CCHC-type / Zinc finger CCHC-type profile. / Tetratricopeptide-like helical domain superfamily
Similarity search - Domain/homology
Cyclic nucleotide-binding domain protein / Oxoglutarate/malate translocator protein, putative / Transmembrane protein / SURF1-like protein / Uncharacterized protein / Transmembrane protein / Transmembrane protein, putative / SURF1-like protein / Transmembrane protein / Iron-binding zinc finger CDGSH type protein ...Cyclic nucleotide-binding domain protein / Oxoglutarate/malate translocator protein, putative / Transmembrane protein / SURF1-like protein / Uncharacterized protein / Transmembrane protein / Transmembrane protein, putative / SURF1-like protein / Transmembrane protein / Iron-binding zinc finger CDGSH type protein / Uncharacterized protein / Uncharacterized protein / Transmembrane protein / Transmembrane protein / Transmembrane protein, putative / Transmembrane protein / Uncharacterized protein / Transmembrane protein, putative / CTF/NF-I domain-containing protein / Transmembrane protein, putative / Protein phosphatase 2C, putative / Uncharacterized protein / Transmembrane protein, putative / Chromosome condensation regulator RCC1 repeat protein / Transmembrane protein, putative / Carrier protein / Uncharacterized protein / Tim10/DDP family zinc finger protein / Uncharacterized protein / Transmembrane protein, putative / Transmembrane protein, putative / TraB family protein / Transmembrane protein, putative / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Cytochrome C oxidase subunit Vb protein / 2-oxoglutarate/malate carrier protein / Transmembrane protein, putative / Transmembrane protein, putative / Uncharacterized protein / Uncharacterized protein / Ymf70 / Cytochrome c oxidase subunit 1 / Ymf68 / Ymf67 / Cytochrome c oxidase polypeptide II / Ymf75 / Transmembrane protein, putative / Transmembrane protein / Mitochondrial import inner membrane translocase subunit / Uncharacterized protein / Transmembrane protein, putative / Tim10/DDP family zinc finger protein / Protein transporter Sec61 alpha subunit
Similarity search - Component
Biological speciesTetrahymena thermophila (eukaryote)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.02 Å
AuthorsZhou L / Maldonado M / Padavannil A / Letts J
Funding support United States, 1 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R35GM137929 United States
CitationJournal: Science / Year: 2022
Title: Structures of 's respiratory chain reveal the diversity of eukaryotic core metabolism.
Authors: Long Zhou / María Maldonado / Abhilash Padavannil / Fei Guo / James A Letts /
Abstract: Respiration is a core biological energy-converting process whose last steps are carried out by a chain of multisubunit complexes in the inner mitochondrial membrane. To probe the functional and ...Respiration is a core biological energy-converting process whose last steps are carried out by a chain of multisubunit complexes in the inner mitochondrial membrane. To probe the functional and structural diversity of eukaryotic respiration, we examined the respiratory chain of the ciliate (Tt). Using cryo-electron microscopy on a mixed sample, we solved structures of a supercomplex between Tt complex I (Tt-CI) and Tt-CIII (Tt-SC I+III) and a structure of Tt-CIV. Tt-SC I+III (~2.3 megadaltons) is a curved assembly with structural and functional symmetry breaking. Tt-CIV is a ~2.7-megadalton dimer with more than 50 subunits per protomer, including mitochondrial carriers and a TIM8-TIM13-like domain. Our structural and functional study of the respiratory chain reveals divergence in key components of eukaryotic respiration, thereby expanding our understanding of core metabolism.
History
DepositionNov 30, 2021-
Header (metadata) releaseApr 6, 2022-
Map releaseApr 6, 2022-
UpdateJun 1, 2022-
Current statusJun 1, 2022Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_32325.png

Downloads & links

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Map

FileDownload / File: emd_32325.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationTetrahymena thermophila electron transport chain complex IV dimer composite map. Combined by Phenix from 3.02 angstrom protomer map focus-refined in cryosparc.
Voxel sizeX=Y=Z: 0.88 Å
Density
Contour LevelBy AUTHOR: 5.0
Minimum - Maximum-22.26331 - 33.16125
Average (Standard dev.)-0.019761126 (±1.2232604)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderZYX
Origin000
Dimensions512512512
Spacing512512512
CellA=B=C: 450.56 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_32325_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half map A for Tetrahymena thermophila electron transport...

Fileemd_32325_half_map_1.map
AnnotationHalf map A for Tetrahymena thermophila electron transport chain complex IV2 protomer map.
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half map A for Tetrahymena thermophila electron transport...

Fileemd_32325_half_map_2.map
AnnotationHalf map A for Tetrahymena thermophila electron transport chain complex IV2 protomer map.
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Cytochrome c oxidase dimer

EntireName: Cytochrome c oxidase dimer
Components
  • Complex: Cytochrome c oxidase dimer
    • Protein or peptide: Unknown peptide
    • Protein or peptide: Transmembrane protein, putative
    • Protein or peptide: Unknown peptide
    • Protein or peptide: Unknown peptide
    • Protein or peptide: Uncharacterized protein
    • Protein or peptide: Protein transporter Sec61 alpha subunit
    • Protein or peptide: Cytochrome c oxidase subunit 1
    • Protein or peptide: Cytochrome c oxidase subunit 2
    • Protein or peptide: Ymf68
    • Protein or peptide: Cytochrome C oxidase subunit Vb protein
    • Protein or peptide: Transmembrane protein, putative
    • Protein or peptide: Cytochrome c oxidase subunit 6B
    • Protein or peptide: Cytochrome c oxidase subunit 6B-like
    • Protein or peptide: Transmembrane protein, putative
    • Protein or peptide: Transmembrane protein, putative
    • Protein or peptide: Cytochrome c oxidase subunit 7C
    • Protein or peptide: CTF/NF-I domain-containing protein
    • Protein or peptide: Oxoglutarate/malate translocator protein, putative
    • Protein or peptide: 2-oxoglutarate/malate carrier protein
    • Protein or peptide: Carrier protein
    • Protein or peptide: Tim10/DDP family zinc finger protein
    • Protein or peptide: Cytochrome c oxidase small TIM subunit 2
    • Protein or peptide: Cytochrome c oxidase small TIM subunit 3
    • Protein or peptide: Cytochrome c oxidase small TIM subunit 4
    • Protein or peptide: Cytochrome c oxidase small TIM subunit 5
    • Protein or peptide: Cytochrome c oxidase small TIM subunit 6
    • Protein or peptide: Chromosome condensation regulator RCC1 repeat protein
    • Protein or peptide: Iron-binding zinc finger CDGSH type protein
    • Protein or peptide: Cytochrome c oxidase acyl carrier-like subunit
    • Protein or peptide: Ymf67
    • Protein or peptide: Ymf70
    • Protein or peptide: Ymf75
    • Protein or peptide: Transmembrane protein, putative
    • Protein or peptide: Protein phosphatase 2C, putative
    • Protein or peptide: Cyclic nucleotide-binding domain protein
    • Protein or peptide: SURF1-like protein
    • Protein or peptide: TraB family protein
    • Protein or peptide: Transmembrane protein, putative
    • Protein or peptide: Cytochrome c oxidase subunit TT7
    • Protein or peptide: SURF1-like protein
    • Protein or peptide: Cytochrome c oxidase subunit TT9
    • Protein or peptide: Cytochrome c oxidase subunit TT10
    • Protein or peptide: Cytochrome c oxidase subunit TT11
    • Protein or peptide: Cytochrome c oxidase subunit TT12
    • Protein or peptide: Transmembrane protein, putative
    • Protein or peptide: Transmembrane protein, putative
    • Protein or peptide: Cytochrome c oxidase subunit TT15
    • Protein or peptide: Cytochrome c oxidase subunit TT16
    • Protein or peptide: Transmembrane protein, putative
    • Protein or peptide: Cytochrome c oxidase subunit TT18
    • Protein or peptide: Cytochrome c oxidase subunit TT19
    • Protein or peptide: Transmembrane protein, putative
    • Protein or peptide: Transmembrane protein, putative
    • Protein or peptide: Cytochrome c oxidase subunit TT22
    • Protein or peptide: Transmembrane protein, putative
    • Protein or peptide: Transmembrane protein, putative
    • Protein or peptide: Cytochrome c oxidase subunit TT25
    • Protein or peptide: Cytochrome c oxidase subunit TT26
  • Ligand: HEME-A
  • Ligand: COPPER (II) ION
  • Ligand: MAGNESIUM ION
  • Ligand: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE
  • Ligand: CARDIOLIPIN
  • Ligand: ZINC ION
  • Ligand: FE2/S2 (INORGANIC) CLUSTER

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Supramolecule #1: Cytochrome c oxidase dimer

SupramoleculeName: Cytochrome c oxidase dimer / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#58
Details: Tetrahymena thermophila mitochondrial cytochrome c oxidase/complex IV dimer
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB255 / Organelle: Mitochondria / Location in cell: Mitochondrial inner membrane
Molecular weightTheoretical: 2.7 MDa

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Macromolecule #1: Unknown peptide

MacromoleculeName: Unknown peptide / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB255
Molecular weightTheoretical: 8.358294 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)

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Macromolecule #2: Transmembrane protein, putative

MacromoleculeName: Transmembrane protein, putative / type: protein_or_peptide / ID: 2 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 417.001562 KDa
SequenceString: MLIQIVLVLC QYFQLISCFC QGRLECLNPI QKRCVQIDGL QFIGREKKTG FCVIDSVLYD EIDFCLSESN NVCITYDRKM CIKVESNNS IVAINQEDSI CGITIDGYCV DENQNYSQEI YCNDSHCKFT NKQYQKSCLN YKSPQIYGKA NKNECVLQDQ Y FHSDAQII ...String:
MLIQIVLVLC QYFQLISCFC QGRLECLNPI QKRCVQIDGL QFIGREKKTG FCVIDSVLYD EIDFCLSESN NVCITYDRKM CIKVESNNS IVAINQEDSI CGITIDGYCV DENQNYSQEI YCNDSHCKFT NKQYQKSCLN YKSPQIYGKA NKNECVLQDQ Y FHSDAQII CKPHYCIYFQ DKQITCVKMD HSIHPQIIGK DSDGICIFQK VEKQDFYLKY RNLQLCADQK HCYDTSQQTC MP LSASYPA VKMGGTCGNY NEPISISCFY EGNVCLLENP RVCIRIFFNQ DYDVCGIRKD GLCAASNIYY QNLQICASDY CMY GQANNA KCIKMDGQQA VGIDSQGFCV QNFEQKADRC STNFSYICYL NSSQICINLQ GYQNYCQQAN KCYEFSLQQY IGRD IYYYC IQSEQSLQPQ NVQNCLQNPS YICQKRDGTM CVYYYQIPTY FTYLGYINSS GYCAQEGYQT YTSQPQFQTI TNLNP NFCQ DDSYVIQKIL PPYYGRLDSY NYNRCLKQSD QSPYIESCIQ GYCISNNSCQ KIGFDSKNVS KLSNQYCGLV QQSSAI ECA YDQDYCIYNS ICYNLSDQNI QTSGIDYYGN CLQRGKYYQN WKKCSVNHCY QKKLLLNSFG CFYLDGTQGA AGIDSNG MC LDYGVSTPVR CALSSQVCLD FQKNTCQQTI NFGSTGNGCY YSGYCYQINL GQFIGRDTSL ICLSDQEATN NLDTCYND N QNICMAVYSS LKFCVVYPRT SLYLGYIVQN KQCAVENQQT NQGQLVDLVN LRNNYCQDQQ GYIRRLDGKQ YVGVDSSQY LCLESGKTPS KSIIQCYTGF CIVENACKAY DQQYVGRDKY YQCLKKGEGI SVECAANYCI DLSTKNCVQI NDTNPAQAGV LDNFQCAHS ERYYYSQILQ CSESYCLQQN PNNPSQQGCF ELDGDINRAG IDSKGNCLQQ DLPNAVRCSP GQFCLNDKFG N ACQSLLLS FKNNRFARQK GTGVCLSYID PSQFKGDNID ICVKGSCKYS DPKNLDFCFS LGQLAFGNQV VGRDINGDCV LK DQQTSTQ IVECFDMIYC ILDLGGGKQK CQLLQEKDLQ FPNLIYKAKN INQTCQDINM PNSMGCLYGL YCINPNDGYK CVL MNDDNK IGRSLVNQQC LQQGELIANY CQPDFCILNG ACVPLSNKFP GKENQTQKCL PEQIEGQYGA SNCYKEGFCL IINP QNQLA SCYQLDYSNP NTIGIQIDTQ HCLKEGETVA IMCAYEKYCI DPVSQSCKIQ DIKQGYCLGK NGMCAKNGSC VYCQV SQCL TNQNSGTCID LQQPPALFCK DINVHSENIF IQNQKGICQY LNSGQCDICP ENYCKFNNMC FGSQDLLKIL KGNQCF VYV PKLKTCQIQD LNTPNSQGNM NCMNNKGFCQ DTTVVKNQCL MCANYYFNPG NQQCFSIEEK ALLSSSQLFF NMQLIYV KQ DCYDQDYCQS IPSLKCPKGC FSCNSPNFCT QCIEGFFLYQ DTSNQQFCIQ CQKQIIQDNT LKIYYNQIPT YSCLDCSS E YGTWNQISSK YRNCQNFIVK LDNLLQITSQ KYAAQNYIIS PISTTQAPYQ LYFHRSRLCP TGCLSCVQIS DSKVMCLQC QDYYINDDNG SCQKCPDFCI NCQYATFISG KVIPKSQIEK NQQKYYYFVT ICLKCSQYYL VSYNLQSCDN CGINCLYCQY ENYKTVLNY NIRQLQYLSY YEFSTQNFIK KCIQCPQGYV VGYDGQFCQK EIQNCRYTTF QIAQSYDLTL YIWSFTQNST I ANQICKQC VDGYILSGDA SSCLDGCQQQ ISDNRCESCY SPLNKIQCQF CNSGQILNQV VNPGACQDDI CSRNIVGCQE CY KYQDPQN PSPFYQIYQC TKCIDQYSIP SIFSCIQCPT GCAKCYEGYR YYNFTSELVY KRTQFTVNQR LNYYQNDYQL YCT ECQKGY QFDQQQKKCI KIQCGPNCNL CILINNQPQC VQCNYDKLIA ELNQLSYFIG ILYFKQNYIP NPKTMISLTS SGND CQICP ILCETCVNNS DVSINPLFIY DAQCLSCKQS LDQSYVLQNY QITYDKSRRK CYLCNKSEQG CYYKKQKVIY AQCLD LSTR LGDGSLQNPI NFSRLNEIDL NQFLINEIEY NQAIIYYNEL QVKELEVQLI FVDGSCYDYR PQKFSINIKD SIRALS TSL NITTLNQNSV LKFYQLDAFT VQGFDQIYIK NIIFLQQRND AKLGLILVDK ALTNIQLFNC QFLQIQQLIQ SIQFMTF NL QTIQQTKVKL EQVVFQNIQI FESTSLININ NTQNSSIYLK MNQVKLNNVL FQQSKLISTN SMNITIDITN LFVNQSLF D QQSLLLDMKQ NLLVDQYIGI LLNNIIFLNS SFLHGSTILN SDFLDFLQID NLTFNNCTFQ QTQKQAVLPL IAANTFMLT QIQIISNTIT NYSFLQQYDN YFKDQNQSSS FSGIDIIDNK VNSDSYLFLF FQTQQNRNSS IHGINILRNQ FSSNYTQVIV QFNQQNFVQ IQNLTIVDNQ QFLFISTDSV RNILLQQVYQ YQTSSQYFAA QICYLRQTKN QINIKNIKLE GIYFQKNIFQ I ENSLNNYL QKDFQITVTN LFSTQSTLLL KRYTNDVTIF SLYSKQQISL TVQNVTFSFF KSQVLNNKTS LGKISLGFYF QG LQIKATI LNSTFADLDI SSPFNWIEGS VQQMILKSCN FTNSNGYQNY NSVRNGGFFK ITSQKLIIKK SIFINGNALN GGA FYLIMQ GKGTLNLINS TFINNKAYSN NDQETKGGAF YLDGFMSESL DVLVFKSIFS KNLALFSGGA INIQSSNQQN AFII QESFF FDNLSCQGSS INIQSSSDFQ SEVVISKTKV NNQIQNTIST VENIANLLSQ YTLLNIQQLS SSQIFIKDVR NVQFF QSRF QIVPYKFKDS NVLKYFKGII FSQMFSIQNV LNYLDINNIY EKSVFYGKLI NVNNVKNIQI QHTQILNNQN IIEDLS HQD NRVSNLASFH SQNCLVFNLT VNQNKCLQCN LGIIEILSSK LDMQNSTFQN NLAQYGSSLQ LQQNKQYIYN LSKLLQS FQ DKLVISNSTF SHNIANVNGG ALYIKESTVF IYQTLFDTNT ARQYGGALFL ENTKKNILTN LINLQNCTFS NNKAKFGG V LASTTGQRVN KYTNNTYKDN KGYQYGENIQ TSPTKFIITV NKTQLTDTQY INIQNHQGGT LRDEILLALC DDQNNYIQN IPQDSFLSIN ITQGSGFLNQ NKIAHKNGIF NLTQQIQVYG YSKQQLLLSI TSDLIQIPVF NKDEIIIGYD SYQLIINIQM VQVCLPGQI PKKFQEKFDL CYDCQDGSYS FQVSDTCQIC PSPQAKCFRD KILLPSNLWR VSQKSIVLFP CKNCVGDIQL Y KKFKKRSL LKIQSTDLNY YCKEGYIGAL CEDCDRNGQH WGDSYFMNLD LKCQRCSQTT FAEIAYPLMI SLIAFILMIF LT YYIQIQV HLKLTKKVLT ILSKKYYGFN DQSIARTIKF LVYQSSIIGI MFWYDCYIPN TFSYIFIDIG NLFTDQIRIS ECF LEQKFS GTSIIYKGIY FLLGTYFAII SCIIIFYMLL RIKFNYQNYL QDILISLSIF VYFSYNSILS KIFRSVIHLL FLKI KKNVI LQNITNRLIA KSLTKKDSQW SIYQFHVMK

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Macromolecule #3: Unknown peptide

MacromoleculeName: Unknown peptide / type: protein_or_peptide / ID: 3 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB255
Molecular weightTheoretical: 2.91158 KDa
SequenceString:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)

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Macromolecule #4: Unknown peptide

MacromoleculeName: Unknown peptide / type: protein_or_peptide / ID: 4 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB255
Molecular weightTheoretical: 2.571161 KDa
SequenceString:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)

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Macromolecule #5: Uncharacterized protein

MacromoleculeName: Uncharacterized protein / type: protein_or_peptide / ID: 5 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 20.909602 KDa
SequenceString:
MIQQMDQYIS MQNESSSEIS DIETRSPSHS FEDNLKYYSD IYEVLSKDQI KQEYEQLQKS NFVKNIIRSF YLFILESGDE IVIESMFEN QEKGIIQIRK EFQTFTRQKK FNQSTLNSLI NSKRFGKIFY YFLKYYIYDW VISRSVKDLK SHVIFITYLK K QLEQNIEN QQFD

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Macromolecule #6: Protein transporter Sec61 alpha subunit

MacromoleculeName: Protein transporter Sec61 alpha subunit / type: protein_or_peptide / ID: 6 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 53.674324 KDa
SequenceString: MGFIDLLKPA FSLIPEVQPA RQYIPKDHKI VWTGITLFIY LVCCQIPLYG VVRAQGSDPF YWMRVILASN RGTLMELGIS PLVTSSMIV QLLVNTRILD FNARVQDDRQ AIQGAQKLLG LIMSMCEAFA YIWSGQYGDP NKIGLGSCLL IFIQLVFSGI V VLLLDDML ...String:
MGFIDLLKPA FSLIPEVQPA RQYIPKDHKI VWTGITLFIY LVCCQIPLYG VVRAQGSDPF YWMRVILASN RGTLMELGIS PLVTSSMIV QLLVNTRILD FNARVQDDRQ AIQGAQKLLG LIMSMCEAFA YIWSGQYGDP NKIGLGSCLL IFIQLVFSGI V VLLLDDML NKGYGLGSGI SLFIATNICE NIVWRSFSPI TITTQQGTEF EGSIINLFHS LLTKDRVSNA LYHSFYRTSA PN LVNLIST LLVFLVVIYL QGVKYDLRIA RRQERNSESS YPIKLFYTSN YPIILQTALV SQIFFFSRIL SSKFKNNFFV KLL GQWQDG SVAGGQDHPI GGFVYFLTAP RDLNQVISDP IHAIFYVLII LTLCGVISRY WIEFSDESTS SVHKKFMEEK IEIP GHRQD SVYRTLDRII PTAATLGGIC VGVLTIVADF LGAIGSGTGI LLAVNIIYGY FEQVKKENKL KKTGIVQQQQ QQM

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Macromolecule #7: Cytochrome c oxidase subunit 1

MacromoleculeName: Cytochrome c oxidase subunit 1 / type: protein_or_peptide / ID: 7 / Number of copies: 2 / Enantiomer: LEVO / EC number: cytochrome-c oxidase
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 80.564945 KDa
SequenceString: MWVDFIDQTK SLKVSVNNYF YYLDRIKKLF TYLNDLRKHI LKKYVYTINH KRIAINYLYF SMVTGLSGAA LATMIRLELA HPGSPFFKG DSLRYLQVVT AHGLIMVFFV VVPILFGGFA NFLIPYHVGS KDVAYPRLNS IGFWIQPCGY ILLAKIGFLR P QFWRYYDK ...String:
MWVDFIDQTK SLKVSVNNYF YYLDRIKKLF TYLNDLRKHI LKKYVYTINH KRIAINYLYF SMVTGLSGAA LATMIRLELA HPGSPFFKG DSLRYLQVVT AHGLIMVFFV VVPILFGGFA NFLIPYHVGS KDVAYPRLNS IGFWIQPCGY ILLAKIGFLR P QFWRYYDK TSFSFPFLEK MKYNQYKEYK NDYLFYLDFL KKEITDDHSF FWKARKVIKL PQYSVFSFVP LKLMMWKTMI NY PESFWYA ASRVVQSRRK KVFVTKCSAR TLTTAGWTFI TPFSSNIKYT GVGSQDILIL SVVFAGISTT ISFTNLLITR RTL AMPGLR HRRVLMPFVT ISIFLTLRML ATITPVLGAA VIMMAFDRHW QTTFFEYAYG GDPILSQHLF WFFGHPEVYV LIIP TFGFI NMIVPHNNTR RVASKHHMIW AIYVMAYMGY LVWGHHMYLV GLDHRSRTMY STITIMISMP ATIKVVNWTL SLVNG ALKI DLPFLFSMSF LLLFLVAGFT GMWLSHVSLN VSMHDTFYVV AHFHIMLSGA AMTGIFSGIY YYFNALFGVK YSRMFG YMH LIYYSGGQWV AFVPLFYLGF SGMPRRIHDY PVVFMGWHSM STTGHFITLV GIIFFFLMMF DSHIERRAST STTLGLP RW YKRISYYIFK IRYLQHTKSK MNGIPGSTVR LMLINRHFVE YEVYEK

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Macromolecule #8: Cytochrome c oxidase subunit 2

MacromoleculeName: Cytochrome c oxidase subunit 2 / type: protein_or_peptide / ID: 8 / Number of copies: 2 / Enantiomer: LEVO / EC number: cytochrome-c oxidase
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 72.388883 KDa
SequenceString: MWGNLWTEAS YQLNFNIGFS SLRSDVLIHL AQWQYWWWFW FALIWSFYYF IILKVARFRV LKMRPKISTS YRPHGKWGDF LACIIPLIW CINILTNSNL ILRLIEWQNE SSLFTVRVRA RQWYWIYKFE LKNFTDILST PKNIGNNRWQ INTFGELQTA D DYLHVLQL ...String:
MWGNLWTEAS YQLNFNIGFS SLRSDVLIHL AQWQYWWWFW FALIWSFYYF IILKVARFRV LKMRPKISTS YRPHGKWGDF LACIIPLIW CINILTNSNL ILRLIEWQNE SSLFTVRVRA RQWYWIYKFE LKNFTDILST PKNIGNNRWQ INTFGELQTA D DYLHVLQL RSQNKWVKNY WNRSLQETGK TNKAHVISPQ EQLRLSLINQ YKSLNLSSSI KHNAPFINRD LYVFDDLFSY NL GDITTKK SLFNDKNSFL TSYSYLNNNS WNNNEFDLID NLPFTTLFDN NDLFNNYKSF FQDSIFNSPK KQLSSDSKQL FKH IIYRSI KNNIIQDYTK LVKHEDFDEY SRWIKRSPGE VLPLRIIKYP LGLETIHNNI FENTNNEGNV ELFRLRFNSN SSKM QHKLV QDTIYLTLKQ KRYNRKKVVA PQIKYYKDDN GNKTDLVKYT GKPYLSNDKL LKQSIYDQTT QYKLIKKNKK RGELI PVTL ARRILRTKKT LVLPAHVNIT LITNSYDIVH SWFIPGLGIK LDCVPGRSTH HTFFIDNVGF YYGQCAEICG RYHHHM PIR VCALPFEHFL LWWNTFGLPK MLNTVSRKRF ETHYELRKYS W

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Macromolecule #9: Ymf68

MacromoleculeName: Ymf68 / type: protein_or_peptide / ID: 9 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 72.782539 KDa
SequenceString: MLICNFLMYS NFSRIYWFDF NGTVNENLPL NYNVLKICRN EINKLEKLNE NNLGTQKNPI KLNLSFEDKH YNTNNLVLDL NSYETFNSK NFISSIFDKT FESLNTVLMA PIYSFLEFKL KLSSTKINTN HYYVINGKLY ITYNDSFKLF TTINDYFNDL N ELSNTKLF ...String:
MLICNFLMYS NFSRIYWFDF NGTVNENLPL NYNVLKICRN EINKLEKLNE NNLGTQKNPI KLNLSFEDKH YNTNNLVLDL NSYETFNSK NFISSIFDKT FESLNTVLMA PIYSFLEFKL KLSSTKINTN HYYVINGKLY ITYNDSFKLF TTINDYFNDL N ELSNTKLF FLYRSFNIYN IKLNSLVDFV FLKLILFIHL LYLKSTNYNR FDYRLKQTDW GFYINNNSNY IQNIFSGLKY IW RGLRFWI IGLLLGLSSI YYLMYVRLLP FNKIIFAWIL VAMFLYWLLS GFVFFVKKYQ YSKFTAAIQR FWKRTYIIFW VIE AGTFSV FFYLTLNASS EPVYMYDQIK IYKTHLFSWR WFLIKLLPSV SIILLGYYLQ LTLKWNLFNK QNTIVLLITL LLLY ILWLE FYQFYHILSF YGNINWAFDY DEYIWTLELD TRRTRLANNY IAICLFAKFW HFVFIFLFWV FFVLRINELG RIRYP LLVA NVQNFIIIYI MSWAYMYPWL KFIFRKYLDV PYYWFYLNGR ELGIRVFFTD LKLFFYGITN RLFDFNPSSI KFEKYP FYY WINSSQLTEF NQYRKFVIRD SIIYSLNNYI I

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Macromolecule #10: Cytochrome C oxidase subunit Vb protein

MacromoleculeName: Cytochrome C oxidase subunit Vb protein / type: protein_or_peptide / ID: 10 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 75.431312 KDa
SequenceString: MKKQKRTQGK QNTKQIKQEK LSSKRKANNQ KEGKKKVKQE DYKEIKQKGK RMLSKIVKAS FSSKGFNLAN AVNTVKSTLN APIKHIKRN IEPTGSNYSR MTNTTEEAFD EVSHEWQALV TSNPFDLNVF NYLENTQTSN FGTVDNPLVV FTSETPFRYV G CTGQMNED ...String:
MKKQKRTQGK QNTKQIKQEK LSSKRKANNQ KEGKKKVKQE DYKEIKQKGK RMLSKIVKAS FSSKGFNLAN AVNTVKSTLN APIKHIKRN IEPTGSNYSR MTNTTEEAFD EVSHEWQALV TSNPFDLNVF NYLENTQTSN FGTVDNPLVV FTSETPFRYV G CTGQMNED DYEGHELLFF LLREGSLQRC MGCGQVFKLV RLRNEYSPEM DYYLSNFHPY EMQEMGESDT TVLMSPYKYA SH YEYTQFE TPSNMVYSMV NPDEHDRLLV DPAYRMERTK ALEEKYKVYT SSLREVEKQF EERYGRAGQI NISKVTYSTL IDV EKAVLK MDRLFRKVAK FENRAFIDRA NHSRREKRML ERAQQRWDSN YSFFTGSLTE EEQKYRDYYE (TPO)ELEAYPEDE GIEQQLDQQ EVLLSGRYDP KLYDFQEGYT KNPEDDQTSL IEKKAFKFRY RLANETSETF QRRNNRMVER QIKRFQQPQY K HAFEQLQK NIAISSNSGN ALHSEYGYLE LLSNESVQLY KDYYE(SEP)DAEE DFKVFENLSS KEKLVMIANF ENNLLPKY D RSEVHLIPKR QWEPAFGVWE NFLYDITEYA SFIAPRGKEI AADYQIQSAI PLTKEELIEA GLYKETIEKK VEPKLEAKK QTKSE

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Macromolecule #11: Transmembrane protein, putative

MacromoleculeName: Transmembrane protein, putative / type: protein_or_peptide / ID: 11 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 15.618258 KDa
SequenceString:
MIWKYLQRTN RGNIIQAGLQ HRKFENLPFK QNFDNLTKAY DLRMWYISNS PHEAKNLEYV NELEALHNEL NYQNSRQFLF RTVSFLLGW ALFYQFYELP KTYDWQDTQE PKHQVPAYGD LEEGGDEGGD D

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Macromolecule #12: Cytochrome c oxidase subunit 6B

MacromoleculeName: Cytochrome c oxidase subunit 6B / type: protein_or_peptide / ID: 12 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 27.946445 KDa
SequenceString: MSSAVEKKDL PADYGKMPAG YNFLTRGKDW REYDKDFILR TDAVWEKFQL EHFFRNYMKC FFFDHGLKKY QMFEPEDMYT VVFEGWALD DLITFPGFTP TGRTNSYQIG LSPRQRTVVP TQTFYQMQDY YMLCGLRFER WFRCDLVYHD QRHTKFDQVK N QKNYKTYP ...String:
MSSAVEKKDL PADYGKMPAG YNFLTRGKDW REYDKDFILR TDAVWEKFQL EHFFRNYMKC FFFDHGLKKY QMFEPEDMYT VVFEGWALD DLITFPGFTP TGRTNSYQIG LSPRQRTVVP TQTFYQMQDY YMLCGLRFER WFRCDLVYHD QRHTKFDQVK N QKNYKTYP CYREYYEAQY ACQDDMFDFL MELAYARRAA DNFESDFASH ELTTLPTFYD TPKAAERKTY TY

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Macromolecule #13: Cytochrome c oxidase subunit 6B-like

MacromoleculeName: Cytochrome c oxidase subunit 6B-like / type: protein_or_peptide / ID: 13 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 10.228691 KDa
SequenceString:
(FME)EVKYRGPSD DKLECEFLEN NLLSCLREKS VQDNVAKMTC RPEFLVWFFL ECPTKAAVYH DPKGLRNIFI QDKIKQ KGS DDGVLSKDD

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Macromolecule #14: Transmembrane protein, putative

MacromoleculeName: Transmembrane protein, putative / type: protein_or_peptide / ID: 14 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 12.786321 KDa
SequenceString:
MGSVWFRNRY WWYRSLYDDY VAREAKLAFG IAAFIWLPHY YWGIHLNRAF EVNFSHRNYA HEWGPRRNRL AHSLEFEQFD MILENWQDL EDEYAQRGDG MLKK

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Macromolecule #15: Transmembrane protein, putative

MacromoleculeName: Transmembrane protein, putative / type: protein_or_peptide / ID: 15 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 16.413645 KDa
SequenceString:
(FME)NRFFKVSSK YQYYKYLEQY DAAFLRKYQS ETHWYLGRRG AWKNLVIKYA GDHISLEEEH NVKYKTHLSF VYLSYR LAW VLFAYVLIYN HFLLGDIGKT FNVGEWDHRL KPSAERDYPT RYESLYILDR TQKW

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Macromolecule #16: Cytochrome c oxidase subunit 7C

MacromoleculeName: Cytochrome c oxidase subunit 7C / type: protein_or_peptide / ID: 16 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 28.746639 KDa
SequenceString: MISKYRYLHC ARKLVKQSVQ AFGGGHHHHE YDWRDDPKVN KDIEEDIRDR GWHPETYDFP YTKKHDDWVF DVTMPSQNYQ TDLTVNIHP ENKKMHVMKQ VMRQSYWDAE HDMAHEYDYE (SEP)EDLDFQCES FKSQHFRKKG PISQYLILGL LPILYFG TE FFYNHYPDED ...String:
MISKYRYLHC ARKLVKQSVQ AFGGGHHHHE YDWRDDPKVN KDIEEDIRDR GWHPETYDFP YTKKHDDWVF DVTMPSQNYQ TDLTVNIHP ENKKMHVMKQ VMRQSYWDAE HDMAHEYDYE (SEP)EDLDFQCES FKSQHFRKKG PISQYLILGL LPILYFG TE FFYNHYPDED YWRVAHPPPL DYPDTDDTDD TETFKDYK(SEP)F TGRRMVDTGI VDPLWYDIRE GKKVYYDWAG VNQP MEDI

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Macromolecule #17: CTF/NF-I domain-containing protein

MacromoleculeName: CTF/NF-I domain-containing protein / type: protein_or_peptide / ID: 17 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 116.81275 KDa
SequenceString: MNKSQDEVKE DYFSIKKKKY NSLDCVLSPI VTRKKQESIN SFQAGLQEDQ NSTAVPESPF IRNCSETLNR SQHKILDHYF EYDYSLKKK IEKIKISLQN KTGTDSSQSL VQKRLDDLRK KTLIYKQFNI QKNNNNINNT GQFFIEKDKQ NNLNLSQPHQ I IKSKQGHK ...String:
MNKSQDEVKE DYFSIKKKKY NSLDCVLSPI VTRKKQESIN SFQAGLQEDQ NSTAVPESPF IRNCSETLNR SQHKILDHYF EYDYSLKKK IEKIKISLQN KTGTDSSQSL VQKRLDDLRK KTLIYKQFNI QKNNNNINNT GQFFIEKDKQ NNLNLSQPHQ I IKSKQGHK RSFTNTLYCS SNDKKIFNIS SQKQNIKTVK QSNNSSMYEE QIKNDQNKKQ NFNKQAKSER KQAEFCDLFY ES MQKDAER QFKSNSDKSK SITQILENNC ENAIVLLNHL IQILTDSIPI SKNSILLDEK LSQCVNLSNN SKISQLMLVI QKL QGKISY YYNDFLKCIR MMKICKNFCQ LFGTLKFKIS CYKYIGLGFQ RLNKPKVALV YFVKMLRQSW FLKQEDQEIN SYDF IGMSY FYLGSIDKAY QYHQMMIDGI KADEEVKKIS NIMLKNRKYS NYPTLQNIRI IQETSDGQPL QKTIRKSKII NPQFQ LTQQ EWGENISSSE DEFELPQMQI AVKNAEEQTF QKKFLSTPWN DRFIQAQIHK LEIAQELNYN KFPIGLVFKN QSVLNF PNS SNQPQLVVGP YANKIYNQEL SSTYSSRQRI SHQTPNREFF NYHSQLQQVK EQTQLNQMKQ QYNQIVDSIE LANHLIV LK NLKKVKDLFQ NLIPYLNEKY YNIKQQNTKS LKQQQNSFDA QNSNQSFLGL QNYSIQINGI LNINNQTDEY VNNQSDRS Y QQQSDKLKLL IKSKSRNHSK EINMTQNYFK SPSNESKQIK NLRVIVDNSN KKKAQQKLNQ EFSMNFCESP KYDNSILSL FINKLSQESA LRLEIRQIGS ASRYQRKKQQ KGKQRKEKYQ KKQEENKLNI KQYKKERYNM ALFFMANTAI SVPSVLDTSP CMKQMLAYE DCVLDANVPQ QEVIHPQWPT IWPRLLTDGK LLEFDKVPFH PENIYNYTYM RPLTKKNKQY LYECEEERFV F KACLRKVI SLKKTDKHTS WDTAEVANLQ LT

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Macromolecule #18: Oxoglutarate/malate translocator protein, putative

MacromoleculeName: Oxoglutarate/malate translocator protein, putative / type: protein_or_peptide / ID: 18 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 40.392234 KDa
SequenceString: MFFELEDIKR RHSLYWDIYN VQGWVRRPDS TLYNNVKRGV TAGVVASLVQ ENITALVENC KLLATKYEKP QNLRQAATFM KEVFKLENY RKAVWNRSQY ALCIGTFDIG ARLATFRWLN NGWQRVFAGF EFNFVRKIPT TMLAALFTAP FSVPFELARM A YYGDKTFP ...String:
MFFELEDIKR RHSLYWDIYN VQGWVRRPDS TLYNNVKRGV TAGVVASLVQ ENITALVENC KLLATKYEKP QNLRQAATFM KEVFKLENY RKAVWNRSQY ALCIGTFDIG ARLATFRWLN NGWQRVFAGF EFNFVRKIPT TMLAALFTAP FSVPFELARM A YYGDKTFP KELQRGYSSY LSALARIPFE EGPYFLFKNS FPLIIRNFFQ TFTLFYTYDF LKDKASFAWR VGEQNEYACK MI IAGISTY LAAVFSYPWM VTREMVDFWP KVPGAPCTFN GNYRKAAVWI WYHEFSGNYF AGFFTKYFWK ASPGMFLTLM LAD KVGLFD QTTVDNFGGA GNNSWEDTFV

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Macromolecule #19: 2-oxoglutarate/malate carrier protein

MacromoleculeName: 2-oxoglutarate/malate carrier protein / type: protein_or_peptide / ID: 19 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 36.118289 KDa
SequenceString: (FME)NYSYKRYWE PSTAEVIGLS LSVNTISAAL TYPIEFVKVR SQIRTEGVGI RSKNLYMGIN PNKVFREIHA TGNGLR GFY QGFESHLIGR LSYLFIRNLT YKIIYDRTKP VKAHNDLSHR EKGVIAGFAG GLAAFLTSPA DLVNTRTIAE GGKPKEW RW GYKGLMDGIN ...String:
(FME)NYSYKRYWE PSTAEVIGLS LSVNTISAAL TYPIEFVKVR SQIRTEGVGI RSKNLYMGIN PNKVFREIHA TGNGLR GFY QGFESHLIGR LSYLFIRNLT YKIIYDRTKP VKAHNDLSHR EKGVIAGFAG GLAAFLTSPA DLVNTRTIAE GGKPKEW RW GYKGLMDGIN KIAATEGGNA ALFRGSYANV LRAVILNISL TGPFDYLNEK IWITFGDMTW NKYAALLWAS FWGSVATL P FDNIRTRLYA QNADPTKNRL TYSGWADAAK KLIQHEGISG FYVGFYAFYI RTFLYAWTTV FITDKITSDW KRKAGLKEW QI

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Macromolecule #20: Carrier protein

MacromoleculeName: Carrier protein / type: protein_or_peptide / ID: 20 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 37.095141 KDa
SequenceString: MANFVIPYLK PVADFWNSLC IDQHQDSLFQ FKGQTGSLGT DWTSKYLRSE QDVYNHKYLQ YHKRVHEAPE LTDVISDNVY RLTLFAGVE RVLSVRQAQA ILKTQFAGAT ENISGAFQTV LNGGIFRRGY FRGALLNLLQ FCGAPYQSLI WSRNSGITNQ V IVSSIFEA ...String:
MANFVIPYLK PVADFWNSLC IDQHQDSLFQ FKGQTGSLGT DWTSKYLRSE QDVYNHKYLQ YHKRVHEAPE LTDVISDNVY RLTLFAGVE RVLSVRQAQA ILKTQFAGAT ENISGAFQTV LNGGIFRRGY FRGALLNLLQ FCGAPYQSLI WSRNSGITNQ V IVSSIFEA FFYPLDTVKT LIYNDVQGKY KGAFHCASQV VQNAGWSRLY AGIFQKLIFN SALIFHLNQV WDGSSQQWAS LA LVAAAYP LLVLKTRFQV AGTPLALATS NEVLKVNRKT LYAGLVPYLI FNTLFAYEFA AWHSSTAQER VIGGLQNAMK QFS SPAAEQ VWSS

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Macromolecule #21: Tim10/DDP family zinc finger protein

MacromoleculeName: Tim10/DDP family zinc finger protein / type: protein_or_peptide / ID: 21 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 8.00899 KDa
SequenceString:
MDTEVSNHNY TQAVAYLNRA TSSCVKKCDS LNNNGSLSSK QESCLKTCAE NHAIATKIHA EYIRKLAESK YL

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Macromolecule #22: Cytochrome c oxidase small TIM subunit 2

MacromoleculeName: Cytochrome c oxidase small TIM subunit 2 / type: protein_or_peptide / ID: 22 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 8.354574 KDa
SequenceString:
MSDKRKIQHE GIIALINYST LCAQKCDVLK GHDDKITDTE EQCLRVCAEK IRQTFEFTND IYLKNPNLTK PN

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Macromolecule #23: Cytochrome c oxidase small TIM subunit 3

MacromoleculeName: Cytochrome c oxidase small TIM subunit 3 / type: protein_or_peptide / ID: 23 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 10.435701 KDa
SequenceString:
MSTRKIFDSE EQSFIRLVDK FYLGLSLTKL CAQSCNLLRN DISGSALTQK EKDCLSICYN NIEKTQSAFY AKVKTTMNLP AVEDDGEEG GDDE

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Macromolecule #24: Cytochrome c oxidase small TIM subunit 4

MacromoleculeName: Cytochrome c oxidase small TIM subunit 4 / type: protein_or_peptide / ID: 24 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 8.109121 KDa
SequenceString:
(FME)EQNTTQVFS DLAYKVCFKV INDKNKPFVL HDEQRLANCL TRYVEAFNVT SEYFFRERAG ETKVTEKQ

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Macromolecule #25: Cytochrome c oxidase small TIM subunit 5

MacromoleculeName: Cytochrome c oxidase small TIM subunit 5 / type: protein_or_peptide / ID: 25 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 8.965917 KDa
SequenceString:
MEDNYAADVQ RQFNRTAFDS LYKICYNSLV QKNGSTIDFQ KQIDCHQRLI QVFAKIAPIV VKVEQDAASS GGAAAGGEDE E

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Macromolecule #26: Cytochrome c oxidase small TIM subunit 6

MacromoleculeName: Cytochrome c oxidase small TIM subunit 6 / type: protein_or_peptide / ID: 26 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 8.183371 KDa
SequenceString:
(FME)DPVLGDVIA TRIYKACFKH VYGKNMKAYS EKDEAKFDQC LTSYVESYKS VTNHFITYLG QLPKKGLSLD GS

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Macromolecule #27: Chromosome condensation regulator RCC1 repeat protein

MacromoleculeName: Chromosome condensation regulator RCC1 repeat protein / type: protein_or_peptide / ID: 27 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 41.202629 KDa
SequenceString: GNLYTWGQYA SGTGFETASA VPRKVDYFSG NVSKVAMGPY HTAVITNDGS LYTFGWGQNG ALGNGAKEFQ LSPSPVSFFN DKKLKVKDV VVGESYTIAV TENGEVYSWG YGGEPSSKIN LDFFRNAILP QRCGALGSGD NKNRLTPQQI ANLKADGYKN I SGGDNFAT ...String:
GNLYTWGQYA SGTGFETASA VPRKVDYFSG NVSKVAMGPY HTAVITNDGS LYTFGWGQNG ALGNGAKEFQ LSPSPVSFFN DKKLKVKDV VVGESYTIAV TENGEVYSWG YGGEPSSKIN LDFFRNAILP QRCGALGSGD NKNRLTPQQI ANLKADGYKN I SGGDNFAT LVNQSGEVIN WGTGLFGSLG NGSDYPLFTP EVNAYFKHLK EHEGLTVQSI KSAGHFSAAL LSNGKLYTFG VN TQGQLGI RENLGHNTDQ NARLPTPVVD RHFVGQKVVD FEVGENTLVF LTDKNEVFFS GLELAYQPIR WEIPTDKKIV KLA ASKDTF AAVTETGKIY QFNEFVGVST NEVGNDYNVA DSKAFEGKVV DLGGSYGIRF AIVN

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Macromolecule #28: Iron-binding zinc finger CDGSH type protein

MacromoleculeName: Iron-binding zinc finger CDGSH type protein / type: protein_or_peptide / ID: 28 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 21.476887 KDa
SequenceString:
MSAILKRAAK YKRVSSILCE GEAHLRDPFT PPPVILKPPA PRKDKKPDDI TDFPAQKLIP LPESIPYQEG KYRPASIPMV AGFFPYNCY LQQGKVYSWC SCGISQSGPW CDGLCNSVVT RCRPVVFNVS QSGYYKICNC KFSANAPFCN NTHRKMVRYH H QTHRGFYE IWGAALFVLG WVYMGFNYYT

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Macromolecule #29: Cytochrome c oxidase acyl carrier-like subunit

MacromoleculeName: Cytochrome c oxidase acyl carrier-like subunit / type: protein_or_peptide / ID: 29 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 14.740897 KDa
SequenceString:
MMQNLKKFMS KTIQVQPVSF NQIPKAFYNF PEYRTGGVQA NPGITAKRII KCIGERLRKY DPARWENVPI TFKTHFRDEN GYSDVATSI QIHDALEREF GIDIKDRLAL VTDVETAFYI VMSHHDPL

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Macromolecule #30: Ymf67

MacromoleculeName: Ymf67 / type: protein_or_peptide / ID: 30 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 54.538531 KDa
SequenceString: MTALFLHILW SISYIIINIL YIFLSLLLSN NNEKIKQYNS NYFIKILLVL FYNKNLSFYK NLLSEDEISK IEFERLKNYP TLVLIHSNL NKLEKRNKII NSFINFKTKY RFYKFISTNF NLQTIIKNCN DKIIFSTLLY IVNLNYSFFY KTIKNTDLIV Y LLANKFSI ...String:
MTALFLHILW SISYIIINIL YIFLSLLLSN NNEKIKQYNS NYFIKILLVL FYNKNLSFYK NLLSEDEISK IEFERLKNYP TLVLIHSNL NKLEKRNKII NSFINFKTKY RFYKFISTNF NLQTIIKNCN DKIIFSTLLY IVNLNYSFFY KTIKNTDLIV Y LLANKFSI LNDNIIVSKF NISKFNDYIK YINNTNSIDT YLENQIILGL NNNTNSNITK NINTKLLNSY SNLKNLVNIT NN TFYLKKI NDNYNTVINS EFLTYLKSNY KISFSASNIV KYLSDKSVNN SVILYLRKNK IFNKSRYSRN RQTYRTGAYW CLY VNIIAV VAFYFWFYKF TMNFGYLWWL LYSLILSFFF SRALKHRFYN PLNVMTEFKN GFMWFIIILI NIFKPLLKLL ENNY INLYN HLVIKYYQSF ICNTLINKKK LEFNYILSSF KFIKELNNII IISLNKLF

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Macromolecule #31: Ymf70

MacromoleculeName: Ymf70 / type: protein_or_peptide / ID: 31 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 10.919931 KDa
SequenceString:
(FME)FRWLFLYWY NSTDTPSAIA KVNLWSYINL RLFKARLSSS IAYYILGLNN LELKKLKIFY KNTYFDYIYL KSIPCL FLI IFFTNLYLFL

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Macromolecule #32: Ymf75

MacromoleculeName: Ymf75 / type: protein_or_peptide / ID: 32 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 23.325801 KDa
SequenceString:
MFLGIFKDVI KLLNKKVVPV YFWFFLYCFL STMDTNIFVS SCSFLKVEVF GKDENTTLVL LFYVFYSLFN FYLSRIKNKN NYLVRKHLY TTELLIELIL FKYKLIILKF SSIKYILNFN VRKFILFNLF LINNYKAYKI NTFFLYIYIY LNNLNIIWYP I FKAYSIFG YYKSTRLNFI DTKNENIKRI KY

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Macromolecule #33: Transmembrane protein, putative

MacromoleculeName: Transmembrane protein, putative / type: protein_or_peptide / ID: 33 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 57.814465 KDa
SequenceString: MLSKVTRRFL NYNQIYCFAS QHGAEHHKLT ASDEAYLNEV RQRYVTPDME KWAYLDYKKH PSTTLSHYDH KSKDYVESER DDYNADVAT NSHNKLIDDF KRNLQMQRKV HDILQKMDRP YLRGVPGVTK NISAGLQDYS APVSKKSQSD PNDFYRDAYR N ENRWIDQS ...String:
MLSKVTRRFL NYNQIYCFAS QHGAEHHKLT ASDEAYLNEV RQRYVTPDME KWAYLDYKKH PSTTLSHYDH KSKDYVESER DDYNADVAT NSHNKLIDDF KRNLQMQRKV HDILQKMDRP YLRGVPGVTK NISAGLQDYS APVSKKSQSD PNDFYRDAYR N ENRWIDQS VFTPKTSKMT HYDVEWPKEL ASRPVTKKFH HDKGYKYDVT TPYDQRYNYV ADRLGHPEIL GNPFERLMRL EG DIYHPNY LDQPFVKVPN ANPNASLNFE EGEVLYENTR LLEWAKFWNY SVVVGYLWCA YFVPYNIFFK THMPLEHAYD NLF FPYFQH THFLWDNNAL HIPTVGGVAI YATYIALSYI NNIWKDYVVR AQFSKDKELL FVTRVSPFGT TEEEVYEVAH LEHL PPSVR SGVKDLSAQD ADGLVDVTCM SSQRSLVFYK GDQYWNPKVY NDFINQTSNL WTRNYTGYNR LEVQNSVEQV KIGFS HSSQ PKLEKK

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Macromolecule #34: Protein phosphatase 2C, putative

MacromoleculeName: Protein phosphatase 2C, putative / type: protein_or_peptide / ID: 34 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 53.011297 KDa
SequenceString: MFRRIISNGA LLSTQTQRWQ DLSKFACLRA SLNKESEKAF QELAKKNNVS PQELVELSKI VSMNLDVLKQ NINSEQFLLE KESTLKRYR QSSIGTRGHL QTVNEAVNTK YPTLAEGLGQ VAGYKEAYQA LREIFVHPSI SVNNLRQGSY GQQFAVDFRT R ADEYVKAL ...String:
MFRRIISNGA LLSTQTQRWQ DLSKFACLRA SLNKESEKAF QELAKKNNVS PQELVELSKI VSMNLDVLKQ NINSEQFLLE KESTLKRYR QSSIGTRGHL QTVNEAVNTK YPTLAEGLGQ VAGYKEAYQA LREIFVHPSI SVNNLRQGSY GQQFAVDFRT R ADEYVKAL LKDHSSNPQA VQTIQEIQHT LHQIIKNYEQ NPASIYARIL TVLQTRGVNT LPVSKTADQK AVATIQKTST PS LTIDQLT VPVQERVQTQ TVFDAELAFI KEANEMIQQN TGNLPWDGGK KKIFQGQANK YLETPYYLLA ALSGLGLLYF LYS GDAKYK TLVLTPVVGI AAFVLLRRNQ ILNRVPTLTE LFLHKDGKFV DAVVSVNGQL ISKNDIPVST LKLYRGDHTV KVNL NDFED ASAKKFLAQQ SGQEGVINVH FSKLRNLAAR NGQVLNLGDT EVVVPFENQA NRIILKQIFK GVEVLPSS

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Macromolecule #35: Cyclic nucleotide-binding domain protein

MacromoleculeName: Cyclic nucleotide-binding domain protein / type: protein_or_peptide / ID: 35 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 172.272797 KDa
SequenceString: MSVPSLEYQK QYIYSMQKSQ CSIQNQSINI IADEQQAPQE SYLSRKCQRH LFETKCVQLE LGLNGDYSLS NNSLFIQNNN TVFDNLQQN TESNIKQTNR SNKERYETQI FEQHPIIKNP KNDQVSSIMN EQEQKKFINT DENVQQTAQV SKNSKQSFKN G IQQVQTLS ...String:
MSVPSLEYQK QYIYSMQKSQ CSIQNQSINI IADEQQAPQE SYLSRKCQRH LFETKCVQLE LGLNGDYSLS NNSLFIQNNN TVFDNLQQN TESNIKQTNR SNKERYETQI FEQHPIIKNP KNDQVSSIMN EQEQKKFINT DENVQQTAQV SKNSKQSFKN G IQQVQTLS IDKSQASIRE QKKISDCNLD KQFIKGDSRL DESSKIPSKC CGWIPEDIIF ENNIHQLNKK VTLVAKNNFS DS KIQNHKE KKYQQNFQKA AEIVNRLLNT SMNRIMRVRQ HVRNFIMLLK LRYLNRKIDD LTDRDYASIN DLSNFYKSHK KKR NSKAFM KHFNFVIKLS QKLPIFMPTD TLRVVWDVIL VLFTYIFLYF YSILMFFNQD NPDTEFIKEF YFCTFFIFLI DVLV NFNTA FFNKDLIIIN RRQIAKQYIF STVFFTDFVS LIVLGIKVIK RSNFIVYNPN HNLLTYCFNM LIFFKVNGIS SKKKG FDYV FTLKESEKHV IKLINQLFSV ITVAHLAAIG WYFLGILQVV NDNQTNWLVK LNISEGVYYQ KYIYSIYWSI TTMTTV GYG DISATNYGEA LYISIVMLLF SCVFAYSINN IGFILQEIEK SSKQLNDKIT TMQRYLNRKK VNISLKSRIR HYLSFLA QE QRDRDKQAED EIISILSNKL REEITVEINS KILNKFNIFS SNFSKQTLDK LVFKMTEVLI NPNEIIFREE QNDDMSIY F IQSGVIEVYQ QSVQKQDKVT VIQTLTDESL FGEISFFSGL SRKASARSIN LSTLYKISRE DFIYIIQENN EDYERFKMI QEQIVFQGEL PLLHVECYNC KKIGHISSSC PKTHQIFDKQ FIILREIYSS FQQRIFFDRT RYKNNLKPLN LIQDNQNVCK ILKQNLQDD NISIEIMFDS CNRYTGSSYY NSEDLEDDEY GQCDEYATSS NSQTNIDNFS NKSSLNNIKN NALQKKKTMK K KEVSKKYL DNNQICENIS SILNSDQYCN SQKVISNNIN DNLEIKKSYV KSFNLSNRQK SLDDFEKIKS SQNLEQNNNF NN SDIQHSK SQIQIKADSE QFQQNIKAYS QTNKNEVDGD QEQSNISQIF KKRKSQKQQE QINNTNTDIK LQTEYPKQQA RFS STSGST LQSQQLKMPS QKVQDELQLN INQNLKGQEF NINQSNKRVS TLLNQISNNH NQILKNCTEQ TNYRNSIDQI LLQG ILVNN LIQSHSSQNI LPIEQSFKYL KKADGDLEKK KNSILLQNFS LNNNKSSINI TKEFIEDQAA VSHKNLSQIK TKQSI GKSS ILNALSNINN IDGNTNDLNN KNNNSKLLQA QNSQITNGNQ LQIVDRLSKL LLNPQLPLLL KLTSVGMSFR EMNSIN SNN AMDMFDKMHN FKKFFPHNNI ENVLNKLKLL QLELKKQKKQ KQINKPRRQN ILFFRYYSNC GSQVHDNLKL LQQEFNI DD YRPTYLSYGV SKRRGQQFPC NKHNLTLYF

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Macromolecule #36: SURF1-like protein

MacromoleculeName: SURF1-like protein / type: protein_or_peptide / ID: 36 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 47.087973 KDa
SequenceString: MYEGRWKMVN QIKQDVQNDI ELILRQMNER IHAMKKKKYN ELKGKIKQKC IDLLIDYAKQ KIGKKNKIKK EGNNIRHNKQ KKLKNIQKK IFKIISTRML QTTLSAFRPR GSNVTGKVAL ATLGALTGYG AFYHYNQYLN LSARWQQIQE NIAKDQPFDV D GFDAKVYP ...String:
MYEGRWKMVN QIKQDVQNDI ELILRQMNER IHAMKKKKYN ELKGKIKQKC IDLLIDYAKQ KIGKKNKIKK EGNNIRHNKQ KKLKNIQKK IFKIISTRML QTTLSAFRPR GSNVTGKVAL ATLGALTGYG AFYHYNQYLN LSARWQQIQE NIAKDQPFDV D GFDAKVYP WVRENNVNDW EYKLVKMRGY FKDQRFFVRR KRDGKEGFLV FAPFVTAVER VNHRLKQKDL LPVEYSVFVN LG WVPVENK KDVELGGEVC PPMDAPTDST LFVNDTFTGF NPDPANPEDT EQVTLTEITG IVRRGEQQDI LARRRNWNKE GIY NWVDLD YMGKIFRLFN LDAINTAYIE RVVPSFEEGE EGLYPIPATK DTFERPLNTP ERHSTFFNFY AATSALSFIS MLLL RR

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Macromolecule #37: TraB family protein

MacromoleculeName: TraB family protein / type: protein_or_peptide / ID: 37 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 45.050922 KDa
SequenceString: (FME)ISARAVHRF LRNPNLETGA AFRAGTRFDP FKNTLTVLKD PQNGRTLYLI GTTNSSTLLA NRTKDLVQKE KPDAVF VQT NKEWWNLAKN IQDVKCQQEL NRYNDLLSQA YTLSLDNTIR NLVFKAKFYS WLFVINWFKA FPDDFHPFIP GLEMKFA IE EANKQNIPVV ...String:
(FME)ISARAVHRF LRNPNLETGA AFRAGTRFDP FKNTLTVLKD PQNGRTLYLI GTTNSSTLLA NRTKDLVQKE KPDAVF VQT NKEWWNLAKN IQDVKCQQEL NRYNDLLSQA YTLSLDNTIR NLVFKAKFYS WLFVINWFKA FPDDFHPFIP GLEMKFA IE EANKQNIPVV LGGLEVDDVT LSALKVEPRL DPFSQLYYGY RALHNSFWRR EHFDNYATLD VVGGEAYAES MDRFRTNW F VKYFEKLAPY QKKIIVDQKD LDLFYALYRD TPGKKIVAVV NQWHVPGIEN HWKSATNTHE PLKAINPIGD MDINKYMES QLVNDTLRAF VSKVGKTEPA TWKNYSTIYH KDNYEAERVR HVAFVDHKDP HMYHGLPQDY DDNIKPKHH

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Macromolecule #38: Transmembrane protein, putative

MacromoleculeName: Transmembrane protein, putative / type: protein_or_peptide / ID: 38 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 41.307387 KDa
SequenceString: MRYLKIEKEK LVSCKKQEQE VQRIRRRKGN QKLNSIAKQQ RVKRRDYQQN IKQNKEVKNP KKLIKQQIIN KVKKRKKMFR GLTKFNKVF ALNSFKNSLV AVPKANLNHV QNMLEENLKY DAQKYNDEVA VIQKTSRIYK PTYTIEFNRE GEVLVYSADP I KNSVVYFK ...String:
MRYLKIEKEK LVSCKKQEQE VQRIRRRKGN QKLNSIAKQQ RVKRRDYQQN IKQNKEVKNP KKLIKQQIIN KVKKRKKMFR GLTKFNKVF ALNSFKNSLV AVPKANLNHV QNMLEENLKY DAQKYNDEVA VIQKTSRIYK PTYTIEFNRE GEVLVYSADP I KNSVVYFK YPYVLYEAAI PLFIWAWIYN PLELSKNAVN SLLIYPNIAW IPRMWYWRSL QYKIQKMYLL RGGKVAKIET QS LAGDRFT SWVETYQFHP LTQDQKNFDN QDNAEFLEDE GQLKYELGVQ LDNLQEMGTT SQDIVINFMK EGTVHHPELF EAI VKGYNI DTSDYVINTA NNLRAREGNH NH

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Macromolecule #39: Cytochrome c oxidase subunit TT7

MacromoleculeName: Cytochrome c oxidase subunit TT7 / type: protein_or_peptide / ID: 39 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 37.55298 KDa
SequenceString: MFLNRLVKET SKAKRLFSMA QNNFARAGPY NPNRYKDYYI PRTLPKNEEI VEFVQSQHSV PASPIRNQRH INPVRESGPL PSYDGTYTM EDIRAVFYNT TVGRDYCYCQ MDPEEIMRRV PGITRKEAEF ITKLGLSPQE QVDFAYIAYN IGLDIFYFTN Q MFVARQVV ...String:
MFLNRLVKET SKAKRLFSMA QNNFARAGPY NPNRYKDYYI PRTLPKNEEI VEFVQSQHSV PASPIRNQRH INPVRESGPL PSYDGTYTM EDIRAVFYNT TVGRDYCYCQ MDPEEIMRRV PGITRKEAEF ITKLGLSPQE QVDFAYIAYN IGLDIFYFTN Q MFVARQVV TNSKGEKVEV LWNAQCYEDI AQLNVGFAPV LESVDYHWEI FLWADPPIKP NNDFDLNVPC TWFEYEQEWW ME SCIQEDQ FNLPEDERPY NTPRNPHCRK ELWRSQDALQ EEELMVNENW YPKNTQYNIY NQPDFIKPKS GSGAAADDIR I

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Macromolecule #40: SURF1-like protein

MacromoleculeName: SURF1-like protein / type: protein_or_peptide / ID: 40 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 35.794367 KDa
SequenceString: MLQQVFGKSK GGEVTNSHLT PKVLNKAQEA EKLASQIQAQ RFSNRLVAFS SQYPRAKLFF AGIGIGTLLY GANQSSKARE NKVATETRK ERMAKPTIQL TGADSQNPPF TEKNINDWLY KTVSITGRPI HGKGMMIPAK SYGLHGFEYL VPFVTKENED G SVQEGLIL ...String:
MLQQVFGKSK GGEVTNSHLT PKVLNKAQEA EKLASQIQAQ RFSNRLVAFS SQYPRAKLFF AGIGIGTLLY GANQSSKARE NKVATETRK ERMAKPTIQL TGADSQNPPF TEKNINDWLY KTVSITGRPI HGKGMMIPAK SYGLHGFEYL VPFVTKENED G SVQEGLIL NLGFIPREYA PIWARARVEN VEEQTFTCVV TDGKHLSEQG GLFASNKPCE NQWEYADLDQ LAKHTGFVNQ EQ VRSCILE HVNTETPNDE RDCRHIDICS DYKEDYPYKF TRSGVLQQPG QMYWDLNKSA SYYSLLGLGC SVFSALLFLA K

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Macromolecule #41: Cytochrome c oxidase subunit TT9

MacromoleculeName: Cytochrome c oxidase subunit TT9 / type: protein_or_peptide / ID: 41 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 29.865686 KDa
SequenceString: MVYHLFERIC NPDNFKLSGE AARVRTLIAA GFSKEEAEQV AWLQNHQVNG KILGLFTGGF ALYCCNNYFH YFERYFPRLR YQPFTKFLA QAATVYFFFK IGDYYFTSRR YGSNDARMNG LMYSNTYYST NKEALIQNFE PLNRKFTEEE VEQFLRNEGR S QEEKRNWI ...String:
MVYHLFERIC NPDNFKLSGE AARVRTLIAA GFSKEEAEQV AWLQNHQVNG KILGLFTGGF ALYCCNNYFH YFERYFPRLR YQPFTKFLA QAATVYFFFK IGDYYFTSRR YGSNDARMNG LMYSNTYYST NKEALIQNFE PLNRKFTEEE VEQFLRNEGR S QEEKRNWI YNPHIHGSTE GEWKADIHEK FDSGKAPWER EHVKAKILET NKAKIDAGEE IQLKPFKTLN HLDKTGLLHR LH PFIWTNN WTLLG

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Macromolecule #42: Cytochrome c oxidase subunit TT10

MacromoleculeName: Cytochrome c oxidase subunit TT10 / type: protein_or_peptide / ID: 42 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 28.101486 KDa
SequenceString: MSSFIQYEFL KIYQGNQKIK NYYKRKRLIF QQKKVLKKKQ KEIQMSTNNL RLKPWFHWTD EERSHAIFSA YEKRILKSED LPSFLRANR INNVSTWVFP LIALPLFNQS IFKLGFAQRI LLTRPAIEWH CFKIATVAAS WLAWLNFSPF YRKLENEKEY L LDTLESRI ...String:
MSSFIQYEFL KIYQGNQKIK NYYKRKRLIF QQKKVLKKKQ KEIQMSTNNL RLKPWFHWTD EERSHAIFSA YEKRILKSED LPSFLRANR INNVSTWVFP LIALPLFNQS IFKLGFAQRI LLTRPAIEWH CFKIATVAAS WLAWLNFSPF YRKLENEKEY L LDTLESRI GINVLDLNDA LPRWTTSQEY NRRTQQLYNQ RNGFFAGLLY PQEESSRPLV DIASFPKNLH KEKLTK

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Macromolecule #43: Cytochrome c oxidase subunit TT11

MacromoleculeName: Cytochrome c oxidase subunit TT11 / type: protein_or_peptide / ID: 43 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 27.034711 KDa
SequenceString: MFGRLVLKQT RRTLFNPVLK NTFCIYQAYQ NPLRHINTGH NPNNVYEDIV MLGDYPVQNR THDKVISQTY VPAIANIAFT HLSKKYPQA GLKVDQLNTL KEKTWNDLGV NIEHEKQEIL VELSEQIFVK ESKLRWVHEQ RQRLAHTTYV FSGLEFQNVK V GFFIDSYN ...String:
MFGRLVLKQT RRTLFNPVLK NTFCIYQAYQ NPLRHINTGH NPNNVYEDIV MLGDYPVQNR THDKVISQTY VPAIANIAFT HLSKKYPQA GLKVDQLNTL KEKTWNDLGV NIEHEKQEIL VELSEQIFVK ESKLRWVHEQ RQRLAHTTYV FSGLEFQNVK V GFFIDSYN FLLQELAHRS NLYQSKDIVG EKSFHEKHLE QQTAPYSGVK SLEEPVSQNK SFINSLMRAI HNH

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Macromolecule #44: Cytochrome c oxidase subunit TT12

MacromoleculeName: Cytochrome c oxidase subunit TT12 / type: protein_or_peptide / ID: 44 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 26.738438 KDa
SequenceString: IKGNQKKQKG KNQSNNNNNI REEGKQIKEM ILPHNNRQLA RQYFDSLPEN DINRKYYEGL KYETPKTFFG RFLNQFNIDA KLDTLSKFY TYQKTIRATQ AELQEDRKSY LTNSLLFTAV SWFSIYQFAR KGAVLPVLRE YGRYFGTHRL FRQYLHTLVL P LLYTEYAL ...String:
IKGNQKKQKG KNQSNNNNNI REEGKQIKEM ILPHNNRQLA RQYFDSLPEN DINRKYYEGL KYETPKTFFG RFLNQFNIDA KLDTLSKFY TYQKTIRATQ AELQEDRKSY LTNSLLFTAV SWFSIYQFAR KGAVLPVLRE YGRYFGTHRL FRQYLHTLVL P LLYTEYAL NQKYYTHMEH LWTVHVNRLN QKILEDPLYT FYPQELNVPK HNIIVPTIFR DTPQ

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Macromolecule #45: Transmembrane protein, putative

MacromoleculeName: Transmembrane protein, putative / type: protein_or_peptide / ID: 45 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 26.601174 KDa
SequenceString: MSLSLFGVKN NWHKNGIWWF SKILNKTVGE ERYDALRVQR RIWSMRFYYA RQQCLYELFV DHPDLAQWTG TYPKVDSSHG FPFYSTYEM YRDFQENTLN SDGSFAQWIT LVCGIYVIHV IYNYMIPYYW VSTPLKNDEF TRLRMKDYIA STVLEEVYGI S YAEWGWLP ...String:
MSLSLFGVKN NWHKNGIWWF SKILNKTVGE ERYDALRVQR RIWSMRFYYA RQQCLYELFV DHPDLAQWTG TYPKVDSSHG FPFYSTYEM YRDFQENTLN SDGSFAQWIT LVCGIYVIHV IYNYMIPYYW VSTPLKNDEF TRLRMKDYIA STVLEEVYGI S YAEWGWLP HDFAYNRMRG LAGYMHPDDP RAMCTSTFHR KHKYIEHEVE KVGDYHHMTY PK

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Macromolecule #46: Transmembrane protein, putative

MacromoleculeName: Transmembrane protein, putative / type: protein_or_peptide / ID: 46 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 24.701131 KDa
SequenceString: MKKGTASEEE LKKLYDPNTF YEHGDNPAFK QFMNIAVENL REGKLTDHRT YVVDTYKKWM YARNWDDFLQ RDCKAITFPR AFALWIVGT LGMATASKWC RQILPVGSHG ITKISQTQFF HQFGPLGTLG AVGFYGLTAY LYYKTTIFTV KKFYSHCILQ E REWIFEQE ...String:
MKKGTASEEE LKKLYDPNTF YEHGDNPAFK QFMNIAVENL REGKLTDHRT YVVDTYKKWM YARNWDDFLQ RDCKAITFPR AFALWIVGT LGMATASKWC RQILPVGSHG ITKISQTQFF HQFGPLGTLG AVGFYGLTAY LYYKTTIFTV KKFYSHCILQ E REWIFEQE RQNPGYGEYF FKDVPLSAEE HFNDLARGEM AKKKFEKPNH EF

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Macromolecule #47: Cytochrome c oxidase subunit TT15

MacromoleculeName: Cytochrome c oxidase subunit TT15 / type: protein_or_peptide / ID: 47 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 23.25766 KDa
SequenceString: MKEKIFNELT RKMKRKEISA KIQREENKQI LIRQRNNKKY IQSIQGIQQE RKKGKLYLVE MATQNVEEMD TIQKMNYEAT VNMGRQDLI TREYTFYSDY EFIPIQEDRK QQMEDALNNL HKIIHPTVTQ LKKKANVQEI QDRVFRKLQG WEGELNTCVF S AKNVRDSN ...String:
MKEKIFNELT RKMKRKEISA KIQREENKQI LIRQRNNKKY IQSIQGIQQE RKKGKLYLVE MATQNVEEMD TIQKMNYEAT VNMGRQDLI TREYTFYSDY EFIPIQEDRK QQMEDALNNL HKIIHPTVTQ LKKKANVQEI QDRVFRKLQG WEGELNTCVF S AKNVRDSN FCADRFTNRI NTEGVEFVKQ ILREY

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Macromolecule #48: Cytochrome c oxidase subunit TT16

MacromoleculeName: Cytochrome c oxidase subunit TT16 / type: protein_or_peptide / ID: 48 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 20.00733 KDa
SequenceString:
(FME)NNTFKFLHQ VISKLTLKAQ VPNYGQYSHS LKRPINPKVV VFGNSSRAYE LISSQFRNFN HVNGLELKGQ EDNIQA NKV AQSVLSINDG FQDGYYITDF PQNSKQAERL DLITDGVNLA LYIKDPSDKV TVTRQQEAID YYRKTGALVE FEVDPRG DL EEQVKQLSNQ VLNGYKH

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Macromolecule #49: Transmembrane protein, putative

MacromoleculeName: Transmembrane protein, putative / type: protein_or_peptide / ID: 49 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 20.346227 KDa
SequenceString:
MDNNYHFWGN GDRQDVSLSY EDYYSILDCL LDEKLSPQGL MKFKNLHEVS MYGVSYVPLY CFPVAYGISH MLTGKVRRGH SGYRNLFSL MSVVLPFTCW YAYTTPIPRR LYTEIICSNN ADGAYVRNRI KQQKPGIWRK LSQQLYNKNF RFPELNQDLT A TEFPLDYV APHKF

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Macromolecule #50: Cytochrome c oxidase subunit TT18

MacromoleculeName: Cytochrome c oxidase subunit TT18 / type: protein_or_peptide / ID: 50 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 20.025109 KDa
SequenceString:
MVFEFLFYNQ QHKTRNGYFI NHDNLMLASL EERKKLIFYF IANQVPEKLD PVDRVKFNEE LSDNLSTKAR LIGSLTGLIG LVGFPYIST RIYSRPVLNI GLSLLICPFL YYVGNQLTYS VWEPKFIANN NTVCELSKKY NFTVFDFAQA KKEAHLKALR T ELVSDNLL YSPGI

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Macromolecule #51: Cytochrome c oxidase subunit TT19

MacromoleculeName: Cytochrome c oxidase subunit TT19 / type: protein_or_peptide / ID: 51 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 19.882758 KDa
SequenceString:
MAIRNFVFKI SNQIQNLAAK RSLAYLNQID SQSVPSRATI NMKDQVTQMQ REIDNMANVI RAQIPDEDRA EFEILKKYYV TGQHDSLVD PQDVLLQLDR IQVLKNLKMI ELNEEAYDPE LVRLEKLKAR VLLEEEGALL EYAHFISKRP YNKPYEKWGV S EEHVKQQI LG

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Macromolecule #52: Transmembrane protein, putative

MacromoleculeName: Transmembrane protein, putative / type: protein_or_peptide / ID: 52 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 18.801604 KDa
SequenceString:
MGFETVVPAP PTRDDELRMI KATEEQFLQQ PRYKLYMNEA HRIAKMNHGD RHNNIRAHFW SNFALGLLIT GPIFIIPFGK AFRNLRSGV PYYFRPKYVF TQKNQYNQDR NWGAMKKQIP LWLGLSTAYA YWFTDFSIND DEWLEKGKVI YPHQTIKVL

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Macromolecule #53: Transmembrane protein, putative

MacromoleculeName: Transmembrane protein, putative / type: protein_or_peptide / ID: 53 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 18.600445 KDa
SequenceString:
MSCTTRRFID EKEKLEYSRG YNQQELEASK LRKDFVKKYI VDFDTTLYKT QVERDWAYIA KREYRYEVQL KSIGYGGALA NAVLLWRIY ANKKMVFWPI PIVGALGYLY FQPVFFQKSN KRFFDMCNVG EEYYLGRERN KILRECNKIL NVEDF

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Macromolecule #54: Cytochrome c oxidase subunit TT22

MacromoleculeName: Cytochrome c oxidase subunit TT22 / type: protein_or_peptide / ID: 54 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 17.701115 KDa
SequenceString:
MGKDQLDFSH FDKAFENKYD IVAPEFGDLH QKRAEFIAKN QGTYRPVPLV PNNIKGLIPK TCRLPATRNW YRRTSSFERN GFFNIHTPV LNTKMIPWLL FIVLTWGWSS FQIGGYNYER FDDNGERRNT LYWKLSPVEF PQSKLWNRPS

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Macromolecule #55: Transmembrane protein, putative

MacromoleculeName: Transmembrane protein, putative / type: protein_or_peptide / ID: 55 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 15.588598 KDa
SequenceString:
MVFHYTNFVQ ETNAWWLRRV RPVYCTVLAY YGWWLYDRYY LFGKNATQDI RKDTTEVWEK RAALNKRNWG YNAHYKPELE RSMKKVLYA DPNYKFPIEW PERYMAETKT LEQVMDEEEN WEYYK

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Macromolecule #56: Transmembrane protein, putative

MacromoleculeName: Transmembrane protein, putative / type: protein_or_peptide / ID: 56 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 14.302487 KDa
SequenceString:
MEPFGTDERN WTHEEKDIIT RFLKYDKHVN LKTAEMVYSA EVESAYFGKA GALAGGVISA LFFNFPIVRN LPIIRRSVIG VLPFLYCYT WGKNTQEELR WLKTFAAYQR FVVYHGQHCK LWV

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Macromolecule #57: Cytochrome c oxidase subunit TT25

MacromoleculeName: Cytochrome c oxidase subunit TT25 / type: protein_or_peptide / ID: 57 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 12.273913 KDa
SequenceString:
MAQTAHQNRY QGGLCYAQCN ELFSFWNPSI QQCWKGCDFG VGRVNDPEGR IEAQQMCKRW AAELYWTYKG ELDTIKDLRV HADMYPTTP QNVYRACLAG VRRQKF

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Macromolecule #58: Cytochrome c oxidase subunit TT26

MacromoleculeName: Cytochrome c oxidase subunit TT26 / type: protein_or_peptide / ID: 58 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote) / Strain: SB210
Molecular weightTheoretical: 10.418697 KDa
SequenceString:
MSSDPFKKVE RDYHNERSVH KHFASYPLKF WWGLNKFETI QGIHSILGNA ADLVVSTLSF IPGVQGRNNA SYIENSIRVT RFRGFDDKT Q

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Macromolecule #59: HEME-A

MacromoleculeName: HEME-A / type: ligand / ID: 59 / Number of copies: 4 / Formula: HEA
Molecular weightTheoretical: 852.837 Da
Chemical component information

ChemComp-HEA:
HEME-A

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Macromolecule #60: COPPER (II) ION

MacromoleculeName: COPPER (II) ION / type: ligand / ID: 60 / Number of copies: 6 / Formula: CU
Molecular weightTheoretical: 63.546 Da
Chemical component information

ChemComp-CU:
COPPER (II) ION

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Macromolecule #61: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 61 / Number of copies: 2 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #62: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE

MacromoleculeName: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / type: ligand / ID: 62 / Number of copies: 34 / Formula: PC1
Molecular weightTheoretical: 790.145 Da
Chemical component information

ChemComp-PC1:
1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / phospholipid*YM

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Macromolecule #63: CARDIOLIPIN

MacromoleculeName: CARDIOLIPIN / type: ligand / ID: 63 / Number of copies: 68 / Formula: CDL
Molecular weightTheoretical: 1.464043 KDa
Chemical component information

ChemComp-CDL:
CARDIOLIPIN / phospholipid*YM

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Macromolecule #64: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 64 / Number of copies: 4 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #65: FE2/S2 (INORGANIC) CLUSTER

MacromoleculeName: FE2/S2 (INORGANIC) CLUSTER / type: ligand / ID: 65 / Number of copies: 4 / Formula: FES
Molecular weightTheoretical: 175.82 Da
Chemical component information

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration5 mg/mL
BufferpH: 7.4
Component:
ConcentrationFormulaName
20.0 mMTris2-Amino-2-(hydroxymethyl)propane-1,3-diol
50.0 mMNaClsodium chloride
5.0 mMMgCl2magnesium chloride
0.002 g/100 mlPMSFPhenylmethanesulfonyl fluoride

Details: Solution were made fresh from concentrated stocks, filtered and de-gassed before equilibration onto Superose6 column
GridModel: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE / Details: 30 mA
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK III
Details: incubation time before blotting: 60s blot time: 9s blot force: 25 offset: -2 incubation after blotting: 0s.

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Electron microscopy

MicroscopeTFS GLACIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Number grids imaged: 1 / Number real images: 17478 / Average exposure time: 3.0 sec. / Average electron dose: 60.0 e/Å2
Electron beamAcceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
Electron opticsCalibrated magnification: 56818 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 3.0 µm / Nominal defocus min: 0.5 µm
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN

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Image processing

Particle selectionNumber selected: 1798857
CTF correctionSoftware - Name: CTFFIND (ver. 4.1)
Startup modelType of model: INSILICO MODEL
In silico model: 3D ab-inito model reconstruction in cryosparc
Final reconstructionNumber classes used: 1 / Applied symmetry - Point group: C1 (asymmetric) / Algorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 3.02 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 3.2.0) / Number images used: 394262
Initial angle assignmentType: COMMON LINE / Software - Name: cryoSPARC (ver. 3.2.0)
Final angle assignmentType: COMMON LINE / Software - Name: cryoSPARC (ver. 3.2.0)
Final 3D classificationNumber classes: 200 / Avg.num./class: 9000 / Software - Name: cryoSPARC (ver. 3.2.0)
FSC plot (resolution estimation)

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Atomic model buiding 1

RefinementSpace: REAL / Protocol: RIGID BODY FIT
Output model

PDB-7w5z:
Cryo-EM structure of Tetrahymena thermophila mitochondrial complex IV, composite dimer model

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