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Structure paper

TitleStructures of 's respiratory chain reveal the diversity of eukaryotic core metabolism.
Journal, issue, pagesScience, Vol. 376, Issue 6595, Page 831-839, Year 2022
Publish dateMay 20, 2022
AuthorsLong Zhou / María Maldonado / Abhilash Padavannil / Fei Guo / James A Letts /
PubMed AbstractRespiration is a core biological energy-converting process whose last steps are carried out by a chain of multisubunit complexes in the inner mitochondrial membrane. To probe the functional and ...Respiration is a core biological energy-converting process whose last steps are carried out by a chain of multisubunit complexes in the inner mitochondrial membrane. To probe the functional and structural diversity of eukaryotic respiration, we examined the respiratory chain of the ciliate (Tt). Using cryo-electron microscopy on a mixed sample, we solved structures of a supercomplex between Tt complex I (Tt-CI) and Tt-CIII (Tt-SC I+III) and a structure of Tt-CIV. Tt-SC I+III (~2.3 megadaltons) is a curved assembly with structural and functional symmetry breaking. Tt-CIV is a ~2.7-megadalton dimer with more than 50 subunits per protomer, including mitochondrial carriers and a TIM8-TIM13-like domain. Our structural and functional study of the respiratory chain reveals divergence in key components of eukaryotic respiration, thereby expanding our understanding of core metabolism.
External linksScience / PubMed:35357889 / PubMed Central
MethodsEM (single particle)
Resolution2.6 - 3.43 Å
Structure data

25882

7tgh

EMDB-25882, PDB-7tgh:
Cryo-EM structure of respiratory super-complex CI+III2 from Tetrahymena thermophila
Method: EM (single particle) / Resolution: 2.6 Å

32325

7w5z

EMDB-32325, PDB-7w5z:
Cryo-EM structure of Tetrahymena thermophila mitochondrial complex IV, composite dimer model
Method: EM (single particle) / Resolution: 3.02 Å

EMDB-32631: Cryo-EM structure of ATP synthase dimer from Tetrahymena thermophila
Method: EM (single particle) / Resolution: 2.92 Å

EMDB-32632: Cryo-EM structure of ATP synthase dimer from Tetrahymena thermophila
Method: EM (single particle) / Resolution: 3.43 Å

Chemicals

ChemComp-CDL:
CARDIOLIPIN / phospholipid*YM

ChemComp-PC1:
1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / phospholipid*YM

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE

ChemComp-U10:
UBIQUINONE-10

ChemComp-HEC:
HEME C

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER

ChemComp-3PE:
1,2-Distearoyl-sn-glycerophosphoethanolamine / phospholipid*YM

ChemComp-ZN:
Unknown entry

ChemComp-NDP:
NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

ChemComp-ZMP:
S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

ChemComp-SF4:
IRON/SULFUR CLUSTER

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE

ChemComp-HEA:
HEME-A

ChemComp-CU:
COPPER (II) ION

ChemComp-MG:
Unknown entry

Source
  • tetrahymena thermophila (eukaryote)
KeywordsMEMBRANE PROTEIN / Mitochondrial respiration / Electron transport chain / Oxidoreductase / Super-complex CI+III2 / ELECTRON TRANSPORT / cytochrome c oxidase / mitochondrial carrier / membrane complex

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