+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-32296 | |||||||||||||||
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Title | CryoEM structure of human Kv4.3 | |||||||||||||||
Map data | ||||||||||||||||
Sample |
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Keywords | Potassium voltage-gated channel / MEMBRANE PROTEIN | |||||||||||||||
Function / homology | Function and homology information ventricular cardiac muscle cell membrane repolarization / A-type (transient outward) potassium channel activity / Phase 1 - inactivation of fast Na+ channels / membrane repolarization during ventricular cardiac muscle cell action potential / membrane repolarization during cardiac muscle cell action potential / potassium ion export across plasma membrane / membrane repolarization / Voltage gated Potassium channels / postsynaptic specialization membrane / voltage-gated potassium channel activity ...ventricular cardiac muscle cell membrane repolarization / A-type (transient outward) potassium channel activity / Phase 1 - inactivation of fast Na+ channels / membrane repolarization during ventricular cardiac muscle cell action potential / membrane repolarization during cardiac muscle cell action potential / potassium ion export across plasma membrane / membrane repolarization / Voltage gated Potassium channels / postsynaptic specialization membrane / voltage-gated potassium channel activity / regulation of heart rate by cardiac conduction / GABA-ergic synapse / voltage-gated potassium channel complex / potassium ion transport / protein homooligomerization / sarcolemma / postsynaptic membrane / transmembrane transporter binding / dendritic spine / neuronal cell body / metal ion binding / plasma membrane Similarity search - Function | |||||||||||||||
Biological species | Homo sapiens (human) | |||||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.0 Å | |||||||||||||||
Authors | Ma DM / Guo JT | |||||||||||||||
Funding support | China, 4 items
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Citation | Journal: Cell Res / Year: 2022 Title: Structural basis for the gating modulation of Kv4.3 by auxiliary subunits. Authors: Demin Ma / Cheng Zhao / Xiaochen Wang / Xiaoxiao Li / Yi Zha / Yan Zhang / Guosheng Fu / Ping Liang / Jiangtao Guo / Dongwu Lai / | |||||||||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_32296.map.gz | 48.6 MB | EMDB map data format | |
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Header (meta data) | emd-32296-v30.xml emd-32296.xml | 10.4 KB 10.4 KB | Display Display | EMDB header |
Images | emd_32296.png | 124.3 KB | ||
Filedesc metadata | emd-32296.cif.gz | 5.3 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-32296 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-32296 | HTTPS FTP |
-Validation report
Summary document | emd_32296_validation.pdf.gz | 477.4 KB | Display | EMDB validaton report |
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Full document | emd_32296_full_validation.pdf.gz | 477 KB | Display | |
Data in XML | emd_32296_validation.xml.gz | 6.2 KB | Display | |
Data in CIF | emd_32296_validation.cif.gz | 7 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-32296 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-32296 | HTTPS FTP |
-Related structure data
Related structure data | 7w3yMC 7w6nC 7w6sC 7w6tC M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_32296.map.gz / Format: CCP4 / Size: 75.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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Voxel size | X=Y=Z: 1.014 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Sample components
-Entire : voltage-gated potassium channel Kv4.3
Entire | Name: voltage-gated potassium channel Kv4.3 |
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Components |
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-Supramolecule #1: voltage-gated potassium channel Kv4.3
Supramolecule | Name: voltage-gated potassium channel Kv4.3 / type: organelle_or_cellular_component / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: Homo sapiens (human) |
-Macromolecule #1: Isoform 2 of Potassium voltage-gated channel subfamily D member 3
Macromolecule | Name: Isoform 2 of Potassium voltage-gated channel subfamily D member 3 type: protein_or_peptide / ID: 1 / Number of copies: 4 / Enantiomer: LEVO |
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Source (natural) | Organism: Homo sapiens (human) |
Molecular weight | Theoretical: 73.159578 KDa |
Recombinant expression | Organism: Homo sapiens (human) |
Sequence | String: MAAGVAAWLP FARAAAIGWM PVANCPMPLA PADKNKRQDE LIVLNVSGRR FQTWRTTLER YPDTLLGSTE KEFFFNEDTK EYFFDRDPE VFRCVLNFYR TGKLHYPRYE CISAYDDELA FYGILPEIIG DCCYEEYKDR KRENAERLMD DNDSENNQES M PSLSFRQT ...String: MAAGVAAWLP FARAAAIGWM PVANCPMPLA PADKNKRQDE LIVLNVSGRR FQTWRTTLER YPDTLLGSTE KEFFFNEDTK EYFFDRDPE VFRCVLNFYR TGKLHYPRYE CISAYDDELA FYGILPEIIG DCCYEEYKDR KRENAERLMD DNDSENNQES M PSLSFRQT MWRAFENPHT STLALVFYYV TGFFIAVSVI TNVVETVPCG TVPGSKELPC GERYSVAFFC LDTACVMIFT VE YLLRLFA APSRYRFIRS VMSIIDVVAI MPYYIGLVMT NNEDVSGAFV TLRVFRVFRI FKFSRHSQGL RILGYTLKSC ASE LGFLLF SLTMAIIIFA TVMFYAEKGS SASKFTSIPA SFWYTIVTMT TLGYGDMVPK TIAGKIFGSI CSLSGVLVIA LPVP VIVSN FSRIYHQNQR ADKRRAQKKA RLARIRVAKT GSSNAYLHSK RNGLLNEALE LTGTPEEEHM GKTTSLIESQ HHHLL HCLE KTTNHEFIDE QMFEQNCMES SMQNYPSTRS PSLSSHPGLT TTCCSRRSKK TTHLPNSNLP ATRLRSMQEL STIHIQ GSE QPSLTTSRSS LNLKADDGLR PNCKTSQITT AIISIPTPPA LTPEGESRPP PASPGPNTNI PSIASNVVKV SALLEGG SS GGWSHPQFEK UniProtKB: Potassium voltage-gated channel subfamily D member 3 |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 8 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average exposure time: 8.0 sec. / Average electron dose: 64.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | C2 aperture diameter: 50.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Startup model | Type of model: PDB ENTRY |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.0 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 86563 |
Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |