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Open data
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Basic information
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Title | CryoEM structure of human KChIP2-Kv4.3 complex | |||||||||||||||
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![]() | Kv channel-interacting protein 2 Potassium voltage-gated channel / MEMBRANE PROTEIN | |||||||||||||||
Function / homology | ![]() ventricular cardiac muscle cell membrane repolarization / ER retention sequence binding / positive regulation of voltage-gated potassium channel activity / Kv4.2-KChIP2 channel complex / A-type (transient outward) potassium channel activity / Phase 1 - inactivation of fast Na+ channels / clustering of voltage-gated potassium channels / positive regulation of potassium ion export across plasma membrane / membrane repolarization during ventricular cardiac muscle cell action potential / potassium channel complex ...ventricular cardiac muscle cell membrane repolarization / ER retention sequence binding / positive regulation of voltage-gated potassium channel activity / Kv4.2-KChIP2 channel complex / A-type (transient outward) potassium channel activity / Phase 1 - inactivation of fast Na+ channels / clustering of voltage-gated potassium channels / positive regulation of potassium ion export across plasma membrane / membrane repolarization during ventricular cardiac muscle cell action potential / potassium channel complex / membrane repolarization during cardiac muscle cell action potential / potassium ion export across plasma membrane / membrane repolarization / Voltage gated Potassium channels / regulation of potassium ion transmembrane transport / postsynaptic specialization membrane / action potential / regulation of heart contraction / voltage-gated potassium channel activity / potassium channel activity / regulation of heart rate by cardiac conduction / detection of calcium ion / GABA-ergic synapse / potassium channel regulator activity / voltage-gated potassium channel complex / muscle contraction / potassium ion transport / protein homooligomerization / sarcolemma / chemical synaptic transmission / postsynaptic membrane / transmembrane transporter binding / dendritic spine / neuronal cell body / synapse / calcium ion binding / dendrite / protein-containing complex binding / signal transduction / identical protein binding / metal ion binding / plasma membrane / cytoplasm Similarity search - Function | |||||||||||||||
Biological species | ![]() | |||||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.8 Å | |||||||||||||||
![]() | Ma DM / Guo JT | |||||||||||||||
Funding support | 4 items
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![]() | ![]() Title: Structural basis for the gating modulation of Kv4.3 by auxiliary subunits. Authors: Demin Ma / Cheng Zhao / Xiaochen Wang / Xiaoxiao Li / Yi Zha / Yan Zhang / Guosheng Fu / Ping Liang / Jiangtao Guo / Dongwu Lai / ![]() | |||||||||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 66.7 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 11.5 KB 11.5 KB | Display Display | ![]() |
Images | ![]() | 127.3 KB | ||
Filedesc metadata | ![]() | 5.5 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 519.1 KB | Display | ![]() |
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Full document | ![]() | 518.6 KB | Display | |
Data in XML | ![]() | 6.2 KB | Display | |
Data in CIF | ![]() | 7.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7w6sMC ![]() 7w3yC ![]() 7w6nC ![]() 7w6tC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Voxel size | X=Y=Z: 1.014 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
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Sample components
-Entire : Complex of voltage-gated potassium channel Kv4.3 with KChIP2
Entire | Name: Complex of voltage-gated potassium channel Kv4.3 with KChIP2 |
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Components |
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-Supramolecule #1: Complex of voltage-gated potassium channel Kv4.3 with KChIP2
Supramolecule | Name: Complex of voltage-gated potassium channel Kv4.3 with KChIP2 type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: ![]() |
-Macromolecule #1: Kv channel-interacting protein 2
Macromolecule | Name: Kv channel-interacting protein 2 / type: protein_or_peptide / ID: 1 / Number of copies: 4 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 32.693582 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: MRGQGRKESL SDSRDLDGSY DQLTGHPPGP TKKALKQRFL KLLPCCGPQA LPSVSETLAA PASLRPHRPR LLDPDSVDDE FELSTVCHR PEGLEQLQEQ TKFTRKELQV LYRGFKNECP SGIVNEENFK QIYSQFFPQG DSSTYATFLF NAFDTNHDGS V SFEDFVAG ...String: MRGQGRKESL SDSRDLDGSY DQLTGHPPGP TKKALKQRFL KLLPCCGPQA LPSVSETLAA PASLRPHRPR LLDPDSVDDE FELSTVCHR PEGLEQLQEQ TKFTRKELQV LYRGFKNECP SGIVNEENFK QIYSQFFPQG DSSTYATFLF NAFDTNHDGS V SFEDFVAG LSVILRGTVD DRLNWAFNLY DLNKDGCITK EEMLDIMKSI YDMMGKYTYP ALREEAPREH VESFFQKMDR NK DGVVTIE EFIESCQKDE NIMRSMQLFD NVILEGGSSG GHHHHHHHH UniProtKB: Kv channel-interacting protein 2 |
-Macromolecule #2: Isoform 2 of Potassium voltage-gated channel subfamily D member 3
Macromolecule | Name: Isoform 2 of Potassium voltage-gated channel subfamily D member 3 type: protein_or_peptide / ID: 2 / Number of copies: 4 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 71.472781 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: MAAGVAAWLP FARAAAIGWM PVANCPMPLA PADKNKRQDE LIVLNVSGRR FQTWRTTLER YPDTLLGSTE KEFFFNEDTK EYFFDRDPE VFRCVLNFYR TGKLHYPRYE CISAYDDELA FYGILPEIIG DCCYEEYKDR KRENAERLMD DNDSENNQES M PSLSFRQT ...String: MAAGVAAWLP FARAAAIGWM PVANCPMPLA PADKNKRQDE LIVLNVSGRR FQTWRTTLER YPDTLLGSTE KEFFFNEDTK EYFFDRDPE VFRCVLNFYR TGKLHYPRYE CISAYDDELA FYGILPEIIG DCCYEEYKDR KRENAERLMD DNDSENNQES M PSLSFRQT MWRAFENPHT STLALVFYYV TGFFIAVSVI TNVVETVPCG TVPGSKELPC GERYSVAFFC LDTACVMIFT VE YLLRLFA APSRYRFIRS VMSIIDVVAI MPYYIGLVMT NNEDVSGAFV TLRVFRVFRI FKFSRHSQGL RILGYTLKSC ASE LGFLLF SLTMAIIIFA TVMFYAEKGS SASKFTSIPA SFWYTIVTMT TLGYGDMVPK TIAGKIFGSI CSLSGVLVIA LPVP VIVSN FSRIYHQNQR ADKRRAQKKA RLARIRVAKT GSSNAYLHSK RNGLLNEALE LTGTPEEEHM GKTTSLIESQ HHHLL HCLE KTTNHEFIDE QMFEQNCMES SMQNYPSTRS PSLSSHPGLT TTCCSRRSKK TTHLPNSNLP ATRLRSMQEL STIHIQ GSE QPSLTTSRSS LNLKADDGLR PNCKTSQITT AIISIPTPPA LTPEGESRPP PASPGPNTNI PSIASNVVKV SAL UniProtKB: Potassium voltage-gated channel subfamily D member 3 |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 8 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average exposure time: 8.0 sec. / Average electron dose: 64.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | C2 aperture diameter: 50.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Startup model | Type of model: PDB ENTRY |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 2.8 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 240437 |
Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |