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Open data
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Basic information
Entry | Database: PDB / ID: 7w6s | |||||||||||||||
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Title | CryoEM structure of human KChIP2-Kv4.3 complex | |||||||||||||||
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![]() | MEMBRANE PROTEIN / Kv channel-interacting protein 2 Potassium voltage-gated channel | |||||||||||||||
Function / homology | ![]() ventricular cardiac muscle cell membrane repolarization / ER retention sequence binding / positive regulation of voltage-gated potassium channel activity / Kv4.2-KChIP2 channel complex / A-type (transient outward) potassium channel activity / Phase 1 - inactivation of fast Na+ channels / clustering of voltage-gated potassium channels / positive regulation of potassium ion export across plasma membrane / membrane repolarization during ventricular cardiac muscle cell action potential / potassium channel complex ...ventricular cardiac muscle cell membrane repolarization / ER retention sequence binding / positive regulation of voltage-gated potassium channel activity / Kv4.2-KChIP2 channel complex / A-type (transient outward) potassium channel activity / Phase 1 - inactivation of fast Na+ channels / clustering of voltage-gated potassium channels / positive regulation of potassium ion export across plasma membrane / membrane repolarization during ventricular cardiac muscle cell action potential / potassium channel complex / membrane repolarization during cardiac muscle cell action potential / potassium ion export across plasma membrane / membrane repolarization / Voltage gated Potassium channels / regulation of potassium ion transmembrane transport / postsynaptic specialization membrane / action potential / regulation of heart contraction / voltage-gated potassium channel activity / potassium channel activity / regulation of heart rate by cardiac conduction / detection of calcium ion / GABA-ergic synapse / potassium channel regulator activity / voltage-gated potassium channel complex / muscle contraction / potassium ion transport / protein homooligomerization / sarcolemma / chemical synaptic transmission / postsynaptic membrane / transmembrane transporter binding / dendritic spine / neuronal cell body / synapse / calcium ion binding / dendrite / protein-containing complex binding / signal transduction / identical protein binding / metal ion binding / plasma membrane / cytoplasm Similarity search - Function | |||||||||||||||
Biological species | ![]() | |||||||||||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.8 Å | |||||||||||||||
![]() | Ma, D.M. / Guo, J.T. | |||||||||||||||
Funding support | 4items
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![]() | ![]() Title: Structural basis for the gating modulation of Kv4.3 by auxiliary subunits. Authors: Demin Ma / Cheng Zhao / Xiaochen Wang / Xiaoxiao Li / Yi Zha / Yan Zhang / Guosheng Fu / Ping Liang / Jiangtao Guo / Dongwu Lai / ![]() | |||||||||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 460.1 KB | Display | ![]() |
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PDB format | ![]() | 367.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 929 KB | Display | ![]() |
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Full document | ![]() | 959.2 KB | Display | |
Data in XML | ![]() | 67.1 KB | Display | |
Data in CIF | ![]() | 102.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 32334MC ![]() 7w3yC ![]() 7w6nC ![]() 7w6tC M: map data used to model this data C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Components
#1: Protein | Mass: 32693.582 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() #2: Protein | Mass: 71472.781 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
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Sample preparation
Component | Name: Complex of voltage-gated potassium channel Kv4.3 with KChIP2 Type: COMPLEX / Entity ID: all / Source: RECOMBINANT |
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Source (natural) | Organism: ![]() |
Source (recombinant) | Organism: ![]() |
Buffer solution | pH: 8 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: ![]() |
Electron lens | Mode: BRIGHT FIELD / Cs: 2.7 mm / C2 aperture diameter: 50 µm |
Image recording | Average exposure time: 8 sec. / Electron dose: 64 e/Å2 / Film or detector model: GATAN K2 SUMMIT (4k x 4k) |
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Processing
Software | Name: PHENIX / Version: 1.16_3549: / Classification: refinement | ||||||||||||||||||||||||
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CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
3D reconstruction | Resolution: 2.8 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 240437 / Symmetry type: POINT | ||||||||||||||||||||||||
Refine LS restraints |
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