EMDB-29269: Human nuclear pre-60S ribosomal subunit (State J2) - Composite map

基本情報

登録情報

データベース: EMDB / ID: EMD-29269

• タイトル: Human nuclear pre-60S ribosomal subunit (State J2) - Composite map
• マップデータ: Human nuclear pre-60S ribosomal subunit (State J2)

試料

• 複合体: Human nuclear pre-60S ribosomal subunit (State J2)
  • タンパク質・ペプチド: x 51種
  • RNA: x 3種
• リガンド: x 4種

• キーワード: Pre-60S ribosomal subunit / Assembly intermediate / Ribosome / Nucleoprotein complex

機能・相同性

分子機能:

protein localization to nucleoplasm / negative regulation of transcription of nucleolar large rRNA by RNA polymerase I / negative regulation of RNA polymerase II regulatory region sequence-specific DNA binding / regulation of RIG-I signaling pathway / basal RNA polymerase II transcription machinery binding / negative regulation of collagen binding / dendrite extension / preribosome binding / lamin filament / regulation of fatty acid biosynthetic process / regulation of megakaryocyte differentiation / PeBoW complex / miRNA-mediated post-transcriptional gene silencing / negative regulation of signal transduction by p53 class mediator / miRNA-mediated gene silencing by inhibition of translation / eukaryotic 80S initiation complex / negative regulation of protein neddylation / translation at presynapse / axial mesoderm development / ribosomal protein import into nucleus / negative regulation of formation of translation preinitiation complex / 90S preribosome assembly / blastocyst formation / positive regulation of protein K63-linked deubiquitination / protein localization to nucleolus / TORC2 complex binding / maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / GAIT complex / middle ear morphogenesis / regulation of glycolytic process / A band / regulation of reactive oxygen species metabolic process / alpha-beta T cell differentiation / cytoplasmic side of rough endoplasmic reticulum membrane / regulation of G1 to G0 transition / positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / regulation of translation involved in cellular response to UV / protein-DNA complex disassembly / positive regulation of DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator / negative regulation of ubiquitin protein ligase activity / regulation of aerobic respiration / maturation of 5.8S rRNA / response to aldosterone / regulation of cyclin-dependent protein serine/threonine kinase activity / negative regulation of cell-cell adhesion / positive regulation of dendritic spine development / homeostatic process / G1 to G0 transition / rRNA metabolic process / negative regulation of DNA replication / macrophage chemotaxis / lung morphogenesis / Protein hydroxylation / mitotic G2 DNA damage checkpoint signaling / ribosomal large subunit binding / Peptide chain elongation / rRNA transcription / preribosome, large subunit precursor / Selenocysteine synthesis / positive regulation of signal transduction by p53 class mediator / nuclear-transcribed mRNA catabolic process / Formation of a pool of free 40S subunits / blastocyst development / ubiquitin ligase inhibitor activity / Eukaryotic Translation Termination / Response of EIF2AK4 (GCN2) to amino acid deficiency / SRP-dependent cotranslational protein targeting to membrane / Viral mRNA Translation / protein localization to nucleus / negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / GTP hydrolysis and joining of the 60S ribosomal subunit / L13a-mediated translational silencing of Ceruloplasmin expression / Major pathway of rRNA processing in the nucleolus and cytosol / protein targeting / negative regulation of protein-containing complex assembly / protein-RNA complex assembly / cellular response to interleukin-4 / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / cellular response to actinomycin D / negative regulation of ubiquitin-dependent protein catabolic process / ribonucleoprotein complex binding / ribosomal subunit export from nucleus / rough endoplasmic reticulum / MDM2/MDM4 family protein binding / negative regulation of proteasomal ubiquitin-dependent protein catabolic process / maturation of LSU-rRNA / negative regulation of protein ubiquitination / DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest / cytosolic ribosome / embryo implantation / translation initiation factor activity / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ossification / assembly of large subunit precursor of preribosome / ribosomal large subunit biogenesis / negative regulation of cell migration / cytosolic ribosome assembly / regulation of signal transduction by p53 class mediator / innate immune response in mucosa

ドメイン・相同性:

MKI67 FHA domain-interacting nucleolar phosphoprotein, FHA Ki67 binding / FHA Ki67 binding domain of hNIFK / Learning associated protein 18-like / LLP homolog / Ribosome biogenesis protein Nop53/GLTSCR2 / Nop53 (60S ribosomal biogenesis) / : / Nucleolar GTP-binding protein 2, N-terminal domain / Nucleolar GTP-binding protein 2 / NGP1NT (NUC091) domain / Zinc-finger double-stranded RNA-binding / Zinc finger, double-stranded RNA binding / : / Pescadillo / Pescadillo N-terminus / Ribosomal protein L24e / GTP-binding protein, orthogonal bundle domain superfamily / Ribosomal biogenesis NSA2 family / Ribosome assembly factor Mrt4 / : / BRCT domain, a BRCA1 C-terminus domain / NOG, C-terminal / Nucleolar GTP-binding protein 1 / NOGCT (NUC087) domain / Nucleolar GTP-binding protein 1, Rossman-fold domain / NOG1, N-terminal helical domain / Nucleolar GTP-binding protein 1 (NOG1) / NOG1 N-terminal helical domain / Circularly permuted (CP)-type guanine nucleotide-binding (G) domain / Circularly permuted (CP)-type guanine nucleotide-binding (G) domain profile. / Matrin/U1-C-like, C2H2-type zinc finger / U1-like zinc finger / OBG-type guanine nucleotide-binding (G) domain / OBG-type guanine nucleotide-binding (G) domain profile. / Translation initiation factor IF6 / eIF-6 family / translation initiation factor 6 / 50S ribosome-binding GTPase / Ribosomal protein L6, N-terminal / Ribosomal protein L6, N-terminal domain / Ribosomal protein L30e / GTP binding domain / Ribosomal protein L2, archaeal-type / Ribosomal L15/L27a, N-terminal / Ribosomal protein L28e / Ribosomal protein L23 / 50S ribosomal protein L10, insertion domain superfamily / Ribosomal L28e/Mak16 / Ribosomal L28e protein family / 60S ribosomal protein L10P, insertion domain / Insertion domain in 60S ribosomal protein L10P / metallochaperone-like domain / TRASH domain / breast cancer carboxy-terminal domain / Ribosomal protein L29e / Ribosomal L29e protein family / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L22e protein family / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal L38e protein family / Ribosomal protein L27e, conserved site / Ribosomal protein L27e signature. / Ribosomal protein L44e signature. / Ribosomal protein L13e / Ribosomal protein L13e / Ribosomal protein L19, eukaryotic / 60S ribosomal protein L18a/ L20, eukaryotes / : / Ribosomal protein L24e, conserved site / Ribosomal protein L19/L19e conserved site / Ribosomal protein L19e signature. / Ribosomal protein L24e signature. / Ribosomal protein L44e / Ribosomal protein L44 / Ribosomal protein L34e, conserved site / Ribosomal protein L34e signature. / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / : / Ribosomal protein L30e signature 1. / Ribosomal protein L6e signature. / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / Ribosomal protein L23/L25, N-terminal / Ribosomal protein L23, N-terminal domain / Ribosomal protein 60S L18 and 50S L18e / Ribosomal protein L30e signature 2. / Eukaryotic Ribosomal Protein L27, KOW domain / Ribosomal protein L30e, conserved site / Ribosomal protein L27e / Ribosomal protein L27e superfamily / Ribosomal L27e protein family / Ribosomal protein L36e signature. / Ribosomal protein L18/L18-A/B/e, conserved site / Ribosomal protein L18e signature.

構成要素:

Large ribosomal subunit protein eL42 / Zinc finger protein 593 / Pescadillo homolog / Ribosome biogenesis protein NSA2 homolog / Large ribosomal subunit protein eL33 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein eL13 / Large ribosomal subunit protein uL11 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein eL22 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein uL29 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein eL21 / Large ribosomal subunit protein eL28 / Large ribosomal subunit protein eL29 / Large ribosomal subunit protein eL34 / Large ribosomal subunit protein eL14 / Eukaryotic translation initiation factor 6 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein eL15 / Large ribosomal subunit protein eL27 / Large ribosomal subunit protein eL43 / Large ribosomal subunit protein eL37 / Large ribosomal subunit protein eL8 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein eL30 / Large ribosomal subunit protein eL39 / Large ribosomal subunit protein eL31 / Large ribosomal subunit protein eL32 / Large ribosomal subunit protein uL5 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein eL38 / Large ribosomal subunit protein eL19 / Large ribosomal subunit protein eL20 / Large ribosomal subunit protein eL6 / Large ribosomal subunit protein eL18 / Nucleolar GTP-binding protein 2 / Ribosomal protein uL30-like / Protein LLP homolog / MKI67 FHA domain-interacting nucleolar phosphoprotein / GTP-binding protein 4 / Ribosome biogenesis protein NOP53 / Probable ribosome biogenesis protein RLP24 / mRNA turnover protein 4 homolog / Large ribosomal subunit protein eL36

• 生物種: Homo sapiens (ヒト) / human (ヒト)
• 手法: 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 2.55 Å
• データ登録者: Vanden Broeck A / Klinge S

資金援助

European Union, 米国, 2件

Organization	Grant number	国
European Molecular Biology Organization (EMBO)	ALTF 711-2019	European Union
The G. Harold and Leila Y. Mathers Foundation	MF-2104-01554	米国

• 引用: ジャーナル: Science / 年: 2023; タイトル: Principles of human pre-60 biogenesis.; 著者: Arnaud Vanden Broeck / Sebastian Klinge / ; 要旨: During the early stages of human large ribosomal subunit (60) biogenesis, an ensemble of assembly factors establishes and fine-tunes the essential RNA functional centers of pre-60 particles by an unknown mechanism. Here, we report a series of cryo-electron microscopy structures of human nucleolar and nuclear pre-60 assembly intermediates at resolutions of 2.5 to 3.2 angstroms. These structures show how protein interaction hubs tether assembly factor complexes to nucleolar particles and how guanosine triphosphatases and adenosine triphosphatase couple irreversible nucleotide hydrolysis steps to the installation of functional centers. Nuclear stages highlight how a conserved RNA-processing complex, the rixosome, couples large-scale RNA conformational changes with pre-ribosomal RNA processing by the RNA degradation machinery. Our ensemble of human pre-60 particles provides a rich foundation with which to elucidate the molecular principles of ribosome formation.

履歴

登録	2022年12月21日	-
ヘッダ(付随情報) 公開	2023年7月12日	-
マップ公開	2023年7月12日	-
更新	2023年7月26日	-
現状	2023年7月26日	処理サイト: RCSB / 状態: 公開

マップ

• ファイル: ダウンロード / ファイル: emd_29269.map.gz / 形式: CCP4 / 大きさ: 421.9 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• 注釈: Human nuclear pre-60S ribosomal subunit (State J2)
• ボクセルのサイズ: X=Y=Z: 1.072 Å

密度

表面レベル	登録者による: 0.85
最小 - 最大	-0.003454865 - 8.591739
平均 (標準偏差)	0.047222767 (±0.18447888)

• 対称性: 空間群: 1

詳細

EMDB XML:

マップ形状	Axis order X Y Z Origin 0 0 0 サイズ 480 480 480 Spacing 480 480 480
セル	A=B=C: 514.56 Å α=β=γ: 90.0 °

添付データ

マスク #1

• ファイル: emd_29269_msk_1.map

ハーフマップ: Half Map 1

• ファイル: emd_29269_half_map_1.map
• 注釈: Half Map 1

ハーフマップ: Half Map 2

• ファイル: emd_29269_half_map_2.map
• 注釈: Half Map 2

試料の構成要素

全体 : Human nuclear pre-60S ribosomal subunit (State J2)

• 全体: 名称: Human nuclear pre-60S ribosomal subunit (State J2)

要素

• 複合体: Human nuclear pre-60S ribosomal subunit (State J2)
  • タンパク質・ペプチド: 60S ribosomal protein L12
  • RNA: 5.8S rRNA
  • RNA: 28S rRNA
  • RNA: 5S rRNA
  • タンパク質・ペプチド: 60S ribosomal protein L11
  • タンパク質・ペプチド: 60S ribosomal protein L13
  • タンパク質・ペプチド: 60S ribosomal protein L13a
  • タンパク質・ペプチド: 60S ribosomal protein L14
  • タンパク質・ペプチド: 60S ribosomal protein L15
  • タンパク質・ペプチド: 60S ribosomal protein L17
  • タンパク質・ペプチド: 60S ribosomal protein L18
  • タンパク質・ペプチド: 60S ribosomal protein L18a
  • タンパク質・ペプチド: 60S ribosomal protein L19
  • タンパク質・ペプチド: 60S ribosomal protein L21
  • タンパク質・ペプチド: 60S ribosomal protein L22
  • タンパク質・ペプチド: 60S ribosomal protein L23
  • タンパク質・ペプチド: 60S ribosomal protein L23a
  • タンパク質・ペプチド: 60S ribosomal protein L26
  • タンパク質・ペプチド: 60S ribosomal protein L27
  • タンパク質・ペプチド: 60S ribosomal protein L27a
  • タンパク質・ペプチド: 60S ribosomal protein L28
  • タンパク質・ペプチド: 60S ribosomal protein L29
  • タンパク質・ペプチド: 60S ribosomal protein L3
  • タンパク質・ペプチド: 60S ribosomal protein L30
  • タンパク質・ペプチド: 60S ribosomal protein L31
  • タンパク質・ペプチド: 60S ribosomal protein L32
  • タンパク質・ペプチド: 60S ribosomal protein L34
  • タンパク質・ペプチド: 60S ribosomal protein L35
  • タンパク質・ペプチド: 60S ribosomal protein L35a
  • タンパク質・ペプチド: 60S ribosomal protein L36
  • タンパク質・ペプチド: 60S ribosomal protein L36a
  • タンパク質・ペプチド: 60S ribosomal protein L37
  • タンパク質・ペプチド: 60S ribosomal protein L37a
  • タンパク質・ペプチド: 60S ribosomal protein L38
  • タンパク質・ペプチド: 60S ribosomal protein L39
  • タンパク質・ペプチド: Nucleolar GTP-binding protein 2
  • タンパク質・ペプチド: Ribosome biogenesis protein NSA2 homolog
  • タンパク質・ペプチド: Protein LLP homolog
  • タンパク質・ペプチド: Ribosome biogenesis protein NOP53
  • タンパク質・ペプチド: Zinc finger protein 593
  • タンパク質・ペプチド: 60S ribosomal protein L4
  • タンパク質・ペプチド: 60S ribosomal protein L5
  • タンパク質・ペプチド: 60S ribosomal protein L6
  • タンパク質・ペプチド: 60S ribosomal protein L7
  • タンパク質・ペプチド: 60S ribosomal protein L7a
  • タンパク質・ペプチド: 60S ribosomal protein L8
  • タンパク質・ペプチド: 60S ribosomal protein L9
  • タンパク質・ペプチド: MKI67 FHA domain-interacting nucleolar phosphoprotein
  • タンパク質・ペプチド: 60S ribosomal protein L7-like 1
  • タンパク質・ペプチド: Eukaryotic translation initiation factor 6
  • タンパク質・ペプチド: Pescadillo homolog
  • タンパク質・ペプチド: mRNA turnover protein 4 homolog
  • タンパク質・ペプチド: GTP-binding protein 4
  • タンパク質・ペプチド: Probable ribosome biogenesis protein RLP24
• リガンド: MAGNESIUM ION
• リガンド: ZINC ION
• リガンド: GUANOSINE-5'-DIPHOSPHATE
• リガンド: POTASSIUM ION

超分子 #1: Human nuclear pre-60S ribosomal subunit (State J2)

• 超分子: 名称: Human nuclear pre-60S ribosomal subunit (State J2) / タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: #1-#54
• 由来(天然): 生物種: Homo sapiens (ヒト) / 株: HEK293F

分子 #1: 60S ribosomal protein L12

• 分子: 名称: 60S ribosomal protein L12 / タイプ: protein_or_peptide / ID: 1 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 17.847619 KDa

配列

文字列:

MPPKFDPNEI KVVYLRCTGG EVGATSALAP KIGPLGLSPK KVGDDIAKAT GDWKGLRITV KLTIQNRQAQ IEVVPSASAL IIKALKEPP RDRKKQKNIK HSGNITFDEI VNIARQMRHR SLARELSGTI KEILGTAQSV GCNVDGRHPH DIIDDINSGA V ECPAS

UniProtKB: Large ribosomal subunit protein uL11

分子 #5: 60S ribosomal protein L11

• 分子: 名称: 60S ribosomal protein L11 / タイプ: protein_or_peptide / ID: 5 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 20.288465 KDa

配列

文字列:

MAQDQGEKEN PMRELRIRKL CLNICVGESG DRLTRAAKVL EQLTGQTPVF SKARYTVRSF GIRRNEKIAV HCTVRGAKAE EILEKGLKV REYELRKNNF SDTGNFGFGI QEHIDLGIKY DPSIGIYGLD FYVVLGRPGF SIADKKRRTG CIGAKHRISK E EAMRWFQQ KYDGIILPGK

UniProtKB: Large ribosomal subunit protein uL5

分子 #6: 60S ribosomal protein L13

• 分子: 名称: 60S ribosomal protein L13 / タイプ: protein_or_peptide / ID: 6 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 24.321682 KDa

配列

文字列:

MAPSRNGMVL KPHFHKDWQR RVATWFNQPA RKIRRRKARQ AKARRIAPRP ASGPIRPIVR CPTVRYHTKV RAGRGFSLEE LRVAGIHKK VARTIGISVD PRRRNKSTES LQANVQRLKE YRSKLILFPR KPSAPKKGDS SAEELKLATQ LTGPVMPVRN V YKKEKARV ITEEEKNFKA FASLRMARAN ARLFGIRAKR AKEAAEQDVE KKK

UniProtKB: Large ribosomal subunit protein eL13

分子 #7: 60S ribosomal protein L13a

• 分子: 名称: 60S ribosomal protein L13a / タイプ: protein_or_peptide / ID: 7 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 23.633412 KDa

配列

文字列:

MAEVQVLVLD GRGHLLGRLA AIVAKQVLLG RKVVVVRCEG INISGNFYRN KLKYLAFLRK RMNTNPSRGP YHFRAPSRIF WRTVRGMLP HKTKRGQAAL DRLKVFDGIP PPYDKKKRMV VPAALKVVRL KPTRKFAYLG RLAHEVGWKY QAVTATLEEK R KEKAKIHY RKKKQLMRLR KQAEKNVEKK IDKYTEVLKT HGLLV

UniProtKB: Large ribosomal subunit protein uL13

分子 #8: 60S ribosomal protein L14

• 分子: 名称: 60S ribosomal protein L14 / タイプ: protein_or_peptide / ID: 8 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 23.485016 KDa

配列

文字列:

MVFRRFVEVG RVAYVSFGPH AGKLVAIVDV IDQNRALVDG PCTQVRRQAM PFKCMQLTDF ILKFPHSAHQ KYVRQAWQKA DINTKWAAT RWAKKIEARE RKAKMTDFDR FKVMKAKKMR NRIIKNEVKK LQKAALLKAS PKKAPGTKGT AAAAAAAAAA K VPAKKITA ASKKAPAQKV PAQKATGQKA APAPKAQKGQ KAPAQKAPAP KASGKKA

UniProtKB: Large ribosomal subunit protein eL14

分子 #9: 60S ribosomal protein L15

• 分子: 名称: 60S ribosomal protein L15 / タイプ: protein_or_peptide / ID: 9 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 24.207285 KDa

配列

文字列:

MGAYKYIQEL WRKKQSDVMR FLLRVRCWQY RQLSALHRAP RPTRPDKARR LGYKAKQGYV IYRIRVRRGG RKRPVPKGAT YGKPVHHGV NQLKFARSLQ SVAEERAGRH CGALRVLNSY WVGEDSTYKF FEVILIDPFH KAIRRNPDTQ WITKPVHKHR E MRGLTSAG RKSRGLGKGH KFHHTIGGSR RAAWRRRNTL QLHRYR

UniProtKB: Large ribosomal subunit protein eL15

分子 #10: 60S ribosomal protein L17

• 分子: 名称: 60S ribosomal protein L17 / タイプ: protein_or_peptide / ID: 10 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 21.44317 KDa

配列

文字列:

MVRYSLDPEN PTKSCKSRGS NLRVHFKNTR ETAQAIKGMH IRKATKYLKD VTLQKQCVPF RRYNGGVGRC AQAKQWGWTQ GRWPKKSAE FLLHMLKNAE SNAELKGLDV DSLVIEHIQV NKAPKMRRRT YRAHGRINPY MSSPCHIEMI LTEKEQIVPK P EEEVAQKK KISQKKLKKQ KLMARE

UniProtKB: Large ribosomal subunit protein uL22

分子 #11: 60S ribosomal protein L18

• 分子: 名称: 60S ribosomal protein L18 / タイプ: protein_or_peptide / ID: 11 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 21.687676 KDa

配列

文字列:

MGVDIRHNKD RKVRRKEPKS QDIYLRLLVK LYRFLARRTN STFNQVVLKR LFMSRTNRPP LSLSRMIRKM KLPGRENKTA VVVGTITDD VRVQEVPKLK VCALRVTSRA RSRILRAGGK ILTFDQLALD SPKGCGTVLL SGPRKGREVY RHFGKAPGTP H SHTKPYVR SKGRKFERAR GRRASRGYKN

UniProtKB: Large ribosomal subunit protein eL18

分子 #12: 60S ribosomal protein L18a

• 分子: 名称: 60S ribosomal protein L18a / タイプ: protein_or_peptide / ID: 12 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 20.808514 KDa

配列

文字列:

MKASGTLREY KVVGRCLPTP KCHTPPLYRM RIFAPNHVVA KSRFWYFVSQ LKKMKKSSGE IVYCGQVFEK SPLRVKNFGI WLRYDSRSG THNMYREYRD LTTAGAVTQC YRDMGARHRA RAHSIQIMKV EEIAASKCRR PAVKQFHDSK IKFPLPHRVL R RQHKPRFT TKRPNTFF

UniProtKB: Large ribosomal subunit protein eL20

分子 #13: 60S ribosomal protein L19

• 分子: 名称: 60S ribosomal protein L19 / タイプ: protein_or_peptide / ID: 13 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 23.535281 KDa

配列

文字列:

MSMLRLQKRL ASSVLRCGKK KVWLDPNETN EIANANSRQQ IRKLIKDGLI IRKPVTVHSR ARCRKNTLAR RKGRHMGIGK RKGTANARM PEKVTWMRRM RILRRLLRRY RESKKIDRHM YHSLYLKVKG NVFKNKRILM EHIHKLKADK ARKKLLADQA E ARRSKTKE ARKRREERLQ AKKEEIIKTL SKEEETKK

UniProtKB: Large ribosomal subunit protein eL19

分子 #14: 60S ribosomal protein L21

• 分子: 名称: 60S ribosomal protein L21 / タイプ: protein_or_peptide / ID: 14 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 18.609988 KDa

配列

文字列:

MTNTKGKRRG TRYMFSRPFR KHGVVPLATY MRIYKKGDIV DIKGMGTVQK GMPHKCYHGK TGRVYNVTQH AVGIVVNKQV KGKILAKRI NVRIEHIKHS KSRDSFLKRV KENDQKKKEA KEKGTWVQLK RQPAPPREAH FVRTNGKEPE LLEPIPYEFM A

UniProtKB: Large ribosomal subunit protein eL21

分子 #15: 60S ribosomal protein L22

• 分子: 名称: 60S ribosomal protein L22 / タイプ: protein_or_peptide / ID: 15 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 14.813015 KDa

配列

文字列:

MAPVKKLVVK GGKKKKQVLK FTLDCTHPVE DGIMDAANFE QFLQERIKVN GKAGNLGGGV VTIERSKSKI TVTSEVPFSK RYLKYLTKK YLKKNNLRDW LRVVANSKES YELRYFQINQ DEEEEEDED

UniProtKB: Large ribosomal subunit protein eL22

分子 #16: 60S ribosomal protein L23

• 分子: 名称: 60S ribosomal protein L23 / タイプ: protein_or_peptide / ID: 16 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 14.892505 KDa

配列

文字列:

MSKRGRGGSS GAKFRISLGL PVGAVINCAD NTGAKNLYII SVKGIKGRLN RLPAAGVGDM VMATVKKGKP ELRKKVHPAV VIRQRKSYR RKDGVFLYFE DNAGVIVNNK GEMKGSAITG PVAKECADLW PRIASNAGSI A

UniProtKB: Large ribosomal subunit protein uL14

分子 #17: 60S ribosomal protein L23a

• 分子: 名称: 60S ribosomal protein L23a / タイプ: protein_or_peptide / ID: 17 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 17.740193 KDa

配列

文字列:

MAPKAKKEAP APPKAEAKAK ALKAKKAVLK GVHSHKKKKI RTSPTFRRPK TLRLRRQPKY PRKSAPRRNK LDHYAIIKFP LTTESAMKK IEDNNTLVFI VDVKANKHQI KQAVKKLYDI DVAKVNTLIR PDGEKKAYVR LAPDYDALDV ANKIGII

UniProtKB: Large ribosomal subunit protein uL23

分子 #18: 60S ribosomal protein L26

• 分子: 名称: 60S ribosomal protein L26 / タイプ: protein_or_peptide / ID: 18 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 17.303363 KDa

配列

文字列:

MKFNPFVTSD RSKNRKRHFN APSHIRRKIM SSPLSKELRQ KYNVRSMPIR KDDEVQVVRG HYKGQQIGKV VQVYRKKYVI YIERVQREK ANGTTVHVGI HPSKVVITRL KLDKDRKKIL ERKAKSRQVG KEKGKYKEET IEKMQE

UniProtKB: Large ribosomal subunit protein uL24

分子 #19: 60S ribosomal protein L27

• 分子: 名称: 60S ribosomal protein L27 / タイプ: protein_or_peptide / ID: 19 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 15.835831 KDa

配列

文字列:

MGKFMKPGKV VLVLAGRYSG RKAVIVKNID DGTSDRPYSH ALVAGIDRYP RKVTAAMGKK KIAKRSKIKS FVKVYNYNHL MPTRYSVDI PLDKTVVNKD VFRDPALKRK ARREAKVKFE ERYKTGKNKW FFQKLRF

UniProtKB: Large ribosomal subunit protein eL27

分子 #20: 60S ribosomal protein L27a

• 分子: 名称: 60S ribosomal protein L27a / タイプ: protein_or_peptide / ID: 20 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 16.604535 KDa

配列

文字列:

MPSRLRKTRK LRGHVSHGHG RIGKHRKHPG GRGNAGGLHH HRINFDKYHP GYFGKVGMKH YHLKRNQSFC PTVNLDKLWT LVSEQTRVN AAKNKTGAAP IIDVVRSGYY KVLGKGKLPK QPVIVKAKFF SRRAEEKIKS VGGACVLVA

UniProtKB: Large ribosomal subunit protein uL15

分子 #21: 60S ribosomal protein L28

• 分子: 名称: 60S ribosomal protein L28 / タイプ: protein_or_peptide / ID: 21 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 15.784622 KDa

配列

文字列:

MSAHLQWMVV RNCSSFLIKR NKQTYSTEPN NLKARNSFRY NGLIHRKTVG VEPAADGKGV VVVIKRRSGQ RKPATSYVRT TINKNARAT LSSIRHMIRK NKYRPDLRMA AIRRASAILR SQKPVMVKRK RTRPTKSS

UniProtKB: Large ribosomal subunit protein eL28

分子 #22: 60S ribosomal protein L29

• 分子: 名称: 60S ribosomal protein L29 / タイプ: protein_or_peptide / ID: 22 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 17.804275 KDa

配列

文字列:

MAKSKNHTTH NQSRKWHRNG IKKPRSQRYE SLKGVDPKFL RNMRFAKKHN KKGLKKMQAN NAKAMSARAE AIKALVKPKE VKPKIPKGV SRKLDRLAYI AHPKLGKRAR ARIAKGLRLC RPKAKAKAKA KDQTKAQAAA PASVPAQAPK RTQAPTKASE

UniProtKB: Large ribosomal subunit protein eL29

分子 #23: 60S ribosomal protein L3

• 分子: 名称: 60S ribosomal protein L3 / タイプ: protein_or_peptide / ID: 23 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 46.224133 KDa

配列

文字列:

MSHRKFSAPR HGSLGFLPRK RSSRHRGKVK SFPKDDPSKP VHLTAFLGYK AGMTHIVREV DRPGSKVNKK EVVEAVTIVE TPPMVVVGI VGYVETPRGL RTFKTVFAEH ISDECKRRFY KNWHKSKKKA FTKYCKKWQD EDGKKQLEKD FSSMKKYCQV I RVIAHTQM RLLPLRQKKA HLMEIQVNGG TVAEKLDWAR ERLEQQVPVN QVFGQDEMID VIGVTKGKGY KGVTSRWHTK KL PRKT(HIC)RG LRKVACIGAW HPARVAFSVA RAGQKGYHHR TEINKKIYKI GQGYLIKDGK LIKNNASTDY DLSDKSINP LGGFVHYGEV TNDFVMLKGC VVGTKKRVLT LRKSLLVQTK RRALEKIDLK FIDTTSKFGH GRFQTMEEKK AFMGPLKKDR IAKEEGA

UniProtKB: Large ribosomal subunit protein uL3

分子 #24: 60S ribosomal protein L30

• 分子: 名称: 60S ribosomal protein L30 / タイプ: protein_or_peptide / ID: 24 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 12.805092 KDa

配列

文字列:

MVAAKKTKKS LESINSRLQL VMKSGKYVLG YKQTLKMIRQ GKAKLVILAN NCPALRKSEI EYYAMLAKTG VHHYSGNNIE LGTACGKYY RVCTLAIIDP GDSDIIRSMP EQTGEK

UniProtKB: Large ribosomal subunit protein eL30

分子 #25: 60S ribosomal protein L31

• 分子: 名称: 60S ribosomal protein L31 / タイプ: protein_or_peptide / ID: 25 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 14.494938 KDa

配列

文字列:

MAPAKKGGEK KKGRSAINEV VTREYTINIH KRIHGVGFKK RAPRALKEIR KFAMKEMGTP DVRIDTRLNK AVWAKGIRNV PYRIRVRLS RKRNEDEDSP NKLYTLVTYV PVTTFKNLQT VNVDEN

UniProtKB: Large ribosomal subunit protein eL31

分子 #26: 60S ribosomal protein L32

• 分子: 名称: 60S ribosomal protein L32 / タイプ: protein_or_peptide / ID: 26 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 15.898932 KDa

配列

文字列:

MAALRPLVKP KIVKKRTKKF IRHQSDRYVK IKRNWRKPRG IDNRVRRRFK GQILMPNIGY GSNKKTKHML PSGFRKFLVH NVKELEVLL MCNKSYCAEI AHNVSSKNRK AIVERAAQLA IRVTNPNARL RSEENE

UniProtKB: Large ribosomal subunit protein eL32

分子 #27: 60S ribosomal protein L34

• 分子: 名称: 60S ribosomal protein L34 / タイプ: protein_or_peptide / ID: 27 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 13.326074 KDa

配列

文字列:

MVQRLTYRRR LSYNTASNKT RLSRTPGNRI VYLYTKKVGK APKSACGVCP GRLRGVRAVR PKVLMRLSKT KKHVSRAYGG SMCAKCVRD RIKRAFLIEE QKIVVKVLKA QAQSQKAK

UniProtKB: Large ribosomal subunit protein eL34

分子 #28: 60S ribosomal protein L35

• 分子: 名称: 60S ribosomal protein L35 / タイプ: protein_or_peptide / ID: 28 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 14.593624 KDa

配列

文字列:

MAKIKARDLR GKKKEELLKQ LDDLKVELSQ LRVAKVTGGA ASKLSKIRVV RKSIARVLTV INQTQKENLR KFYKGKKYKP LDLRPKKTR AMRRRLNKHE ENLKTKKQQR KERLYPLRKY AVKA

UniProtKB: Large ribosomal subunit protein uL29

分子 #29: 60S ribosomal protein L35a

• 分子: 名称: 60S ribosomal protein L35a / タイプ: protein_or_peptide / ID: 29 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 12.564743 KDa

配列

文字列:

MSGRLWSKAI FAGYKRGLRN QREHTALLKI EGVYARDETE FYLGKRCAYV YKAKNNTVTP GGKPNKTRVI WGKVTRAHGN SGMVRAKFR SNLPAKAIGH RIRVMLYPSR I

UniProtKB: Large ribosomal subunit protein eL33

分子 #30: 60S ribosomal protein L36

• 分子: 名称: 60S ribosomal protein L36 / タイプ: protein_or_peptide / ID: 30 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 12.290859 KDa

配列

文字列:

MALRYPMAVG LNKGHKVTKN VSKPRHSRRR GRLTKHTKFV RDMIREVCGF APYERRAMEL LKVSKDKRAL KFIKKRVGTH IRAKRKREE LSNVLAAMRK AAAKKD

UniProtKB: Large ribosomal subunit protein eL36

分子 #31: 60S ribosomal protein L36a

• 分子: 名称: 60S ribosomal protein L36a / タイプ: protein_or_peptide / ID: 31 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 12.476973 KDa

配列

文字列:

MVNVPKTRRT FCKKCGKHQP HKVTQYKKGK DSLYAQGKRR YDRKQSGYGG QTKPIFRKKA KTTKKIVLRL ECVEPNCRSK RMLAIKRCK HFELGGDKKR KGQVIQF

UniProtKB: Large ribosomal subunit protein eL42

分子 #32: 60S ribosomal protein L37

• 分子: 名称: 60S ribosomal protein L37 / タイプ: protein_or_peptide / ID: 32 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 11.111032 KDa

配列

文字列:

MTKGTSSFGK RRNKTHTLCR RCGSKAYHLQ KSTCGKCGYP AKRKRKYNWS AKAKRRNTTG TGRMRHLKIV YRRFRHGFRE GTTPKPKRA AVAASSSS

UniProtKB: Large ribosomal subunit protein eL37

分子 #33: 60S ribosomal protein L37a

• 分子: 名称: 60S ribosomal protein L37a / タイプ: protein_or_peptide / ID: 33 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 10.29935 KDa

配列

文字列:

MAKRTKKVGI VGKYGTRYGA SLRKMVKKIE ISQHAKYTCS FCGKTKMKRR AVGIWHCGSC MKTVAGGAWT YNTTSAVTVK SAIRRLKEL KDQ

UniProtKB: Large ribosomal subunit protein eL43

分子 #34: 60S ribosomal protein L38

• 分子: 名称: 60S ribosomal protein L38 / タイプ: protein_or_peptide / ID: 34 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 8.238948 KDa

配列

文字列:

MPRKIEEIKD FLLTARRKDA KSVKIKKNKD NVKFKVRCSR YLYTLVITDK EKAEKLKQSL PPGLAVKELK

UniProtKB: Large ribosomal subunit protein eL38

分子 #35: 60S ribosomal protein L39

• 分子: 名称: 60S ribosomal protein L39 / タイプ: protein_or_peptide / ID: 35 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 6.426759 KDa

配列

文字列:

MSSHKTFRIK RFLAKKQKQN RPIPQWIRMK TGNKIRYNSK RRHWRRTKLG L

UniProtKB: Large ribosomal subunit protein eL39

分子 #36: Nucleolar GTP-binding protein 2

• 分子: 名称: Nucleolar GTP-binding protein 2 / タイプ: protein_or_peptide / ID: 36 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 83.796094 KDa

配列

文字列:

MVKPKYKGRS TINPSKASTN PDRVQGAGGQ NMRDRATIRR LNMYRQKERR NSRGKIIKPL QYQSTVASGT VARVEPNIKW FGNTRVIKQ SSLQKFQEEM DTVMKDPYKV VMKQSKLPMS LLHDRIRPHN LKVHILDTES FETTFGPKSQ RKRPNLFASD M QSLIENAE MSTESYDQGK DRDLVTEDTG VRNEAQEEIY KKGQSKRIWG ELYKVIDSSD VVVQVLDARD PMGTRSPHIE TY LKKEKPW KHLIFVLNKC DLVPTWATKR WVAVLSQDYP TLAFHASLTN PFGKGAFIQL LRQFGKLHTD KKQISVGFIG YPN VGKSSV INTLRSKKVC NVAPIAGETK VWQYITLMRR IFLIDCPGVV YPSEDSETDI VLKGVVQVEK IKSPEDHIGA VLER AKPEY ISKTYKIDSW ENAEDFLEKL AFRTGKLLKG GEPDLQTVGK MVLNDWQRGR IPFFVKPPNA EPLVAPQLLP SSSLE VVPE AAQNNPGEEV TETAGEGSES IIKEETEENS HCDANTEMQQ ILTRVRQNFG KINVVPQFSG DDLVPVEVSD LEEELE SFS DEEEEEQEQQ RDDAEESSSE PEEENVGNDT KAVIKALDEK IAKYQKFLDK AKAKKFSAVR ISKGLSEKIF AKPEEQR KT LEEDVDDRAP SKKGKKRKAQ REEEQEHSNK APRALTSKER RRAVRQQRPK KVGVRYYETH NVKNRNRNKK KTNDSEGQ K HKRKKFRQKQ

UniProtKB: Nucleolar GTP-binding protein 2

分子 #37: Ribosome biogenesis protein NSA2 homolog

• 分子: 名称: Ribosome biogenesis protein NSA2 homolog / タイプ: protein_or_peptide / ID: 37 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 30.136703 KDa

配列

文字列:

MPQNEYIELH RKRYGYRLDY HEKKRKKESR EAHERSKKAK KMIGLKAKLY HKQRHAEKIQ MKKTIKMHEK RNTKQKNDEK TPQGAVPAY LLDREGQSRA KVLSNMIKQK RKEKAGKWEV PLPKVRAQGE TEVLKVIRTG KRKKKAWKRM VTKVCFVGDG F TRKPPKYE RFIRPMGLRF KKAHVTHPEL KATFCLPILG VKKNPSSPLY TTLGVITKGT VIEVNVSELG LVTQGGKVIW GK YAQVTNN PENDGCINAV LLV

UniProtKB: Ribosome biogenesis protein NSA2 homolog

分子 #38: Protein LLP homolog

• 分子: 名称: Protein LLP homolog / タイプ: protein_or_peptide / ID: 38 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 15.268361 KDa

配列

文字列:

MAKSLRSKWK RKMRAEKRKK NAPKEASRLK SILKLDGDVL MKDVQEIATV VVPKPKHCQE KMQCEVKDEK DDMKMETDIK RNKKTLLDQ HGQYPIWMNQ RQRKRLKAKR EKRKGKSKAK AVKVAKGLAW

UniProtKB: Protein LLP homolog

分子 #39: Ribosome biogenesis protein NOP53

• 分子: 名称: Ribosome biogenesis protein NOP53 / タイプ: protein_or_peptide / ID: 39 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 54.498402 KDa

配列

文字列:

MAAGGSGVGG KRSSKSDADS GFLGLRPTSV DPALRRRRRG PRNKKRGWRR LAQEPLGLEV DQFLEDVRLQ ERTSGGLLSE APNEKLFFV DTGSKEKGLT KKRTKVQKKS LLLKKPLRVD LILENTSKVP APKDVLAHQV PNAKKLRRKE QLWEKLAKQG E LPREVRRA QARLLNPSAT RAKPGPQDTV ERPFYDLWAS DNPLDRPLVG QDEFFLEQTK KKGVKRPARL HTKPSQAPAV EV APAGASY NPSFEDHQTL LSAAHEVELQ RQKEAEKLER QLALPATEQA ATQESTFQEL CEGLLEESDG EGEPGQGEGP EAG DAEVCP TPARLATTEK KTEQQRRREK AVHRLRVQQA ALRAARLRHQ ELFRLRGIKA QVALRLAELA RRQRRRQARR EAEA DKPRR LGRLKYQAPD IDVQLSSELT DSLRTLKPEG NILRDRFKSF QRRNMIEPRE RAKFKRKYKV KLVEKRAFRE IQL

UniProtKB: Ribosome biogenesis protein NOP53

分子 #40: Zinc finger protein 593

• 分子: 名称: Zinc finger protein 593 / タイプ: protein_or_peptide / ID: 40 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 15.230225 KDa

配列

文字列:

MGRSRRTGAH RAHSLARQMK AKRRRPDLDE IHRELRPQGS ARPQPDPNAE FDPDLPGGGL HRCLACARYF IDSTNLKTHF RSKDHKKRL KQLSVEPYSQ EEAERAAGMG SYVPPRRLAV PTEVSTEVPE MDTST

UniProtKB: Zinc finger protein 593

分子 #41: 60S ribosomal protein L4

• 分子: 名称: 60S ribosomal protein L4 / タイプ: protein_or_peptide / ID: 41 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 47.804621 KDa

配列

文字列:

MACARPLISV YSEKGESSGK NVTLPAVFKA PIRPDIVNFV HTNLRKNNRQ PYAVSELAGH QTSAESWGTG RAVARIPRVR GGGTHRSGQ GAFGNMCRGG RMFAPTKTWR RWHRRVNTTQ KRYAICSALA ASALPALVMS KGHRIEEVPE LPLVVEDKVE G YKKTKEAV LLLKKLKAWN DIKKVYASQR MRAGKGKMRN RRRIQRRGPC IIYNEDNGII KAFRNIPGIT LLNVSKLNIL KL APGGHVG RFCIWTESAF RKLDELYGTW RKAASLKSNY NLPMHKMINT DLSRILKSPE IQRALRAPRK KIHRRVLKKN PLK NLRIML KLNPYAKTMR RNTILRQARN HKLRVDKAAA AAAALQAKSD EKAAVAGKKP VVGKKGKKAA VGVKKQKKPL VGKK AAATK KPAPEKKPAE KKPTTEEKKP AA

UniProtKB: Large ribosomal subunit protein uL4

分子 #42: 60S ribosomal protein L5

• 分子: 名称: 60S ribosomal protein L5 / タイプ: protein_or_peptide / ID: 42 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 34.426789 KDa

配列

文字列:

MGFVKVVKNK AYFKRYQVKF RRRREGKTDY YARKRLVIQD KNKYNTPKYR MIVRVTNRDI ICQIAYARIE GDMIVCAAYA HELPKYGVK VGLTNYAAAY CTGLLLARRL LNRFGMDKIY EGQVEVTGDE YNVESIDGQP GAFTCYLDAG LARTTTGNKV F GALKGAVD GGLSIPHSTK RFPGYDSESK EFNAEVHRKH IMGQNVADYM RYLMEEDEDA YKKQFSQYIK NSVTPDMMEE MY KKAHAAI RENPVYEKKP KKEVKKKRWN RPKMSLAQKK DRVAQKKASF LRAQERAAES

UniProtKB: Large ribosomal subunit protein uL18

分子 #43: 60S ribosomal protein L6

• 分子: 名称: 60S ribosomal protein L6 / タイプ: protein_or_peptide / ID: 43 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 32.810176 KDa

配列

文字列:

MAGEKVEKPD TKEKKPEAKK VDAGGKVKKG NLKAKKPKKG KPHCSRNPVL VRGIGRYSRS AMYSRKAMYK RKYSAAKSKV EKKKKEKVL ATVTKPVGGD KNGGTRVVKL RKMPRYYPTE DVPRKLLSHG KKPFSQHVRK LRASITPGTI LIILTGRHRG K RVVFLKQL ASGLLLVTGP LVLNRVPLRR THQKFVIATS TKIDISNVKI PKHLTDAYFK KKKLRKPRHQ EGEIFDTEKE KY EITEQRK IDQKAVDSQI LPKIKAIPQL QGYLRSVFAL TNGIYPHKLV F

UniProtKB: Large ribosomal subunit protein eL6

分子 #44: 60S ribosomal protein L7

• 分子: 名称: 60S ribosomal protein L7 / タイプ: protein_or_peptide / ID: 44 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 29.290973 KDa

配列

文字列:

MEGVEEKKKE VPAVPETLKK KRRNFAELKI KRLRKKFAQK MLRKARRKLI YEKAKHYHKE YRQMYRTEIR MARMARKAGN FYVPAEPKL AFVIRIRGIN GVSPKVRKVL QLLRLRQIFN GTFVKLNKAS INMLRIVEPY IAWGYPNLKS VNELIYKRGY G KINKKRIA LTDNALIARS LGKYGIICME DLIHEIYTVG KRFKEANNFL WPFKLSSPRG GMKKKTTHFV EGGDAGNRED QI NRLIRRM N

UniProtKB: Large ribosomal subunit protein uL30

分子 #45: 60S ribosomal protein L7a

• 分子: 名称: 60S ribosomal protein L7a / タイプ: protein_or_peptide / ID: 45 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 30.061785 KDa

配列

文字列:

MPKGKKAKGK KVAPAPAVVK KQEAKKVVNP LFEKRPKNFG IGQDIQPKRD LTRFVKWPRY IRLQRQRAIL YKRLKVPPAI NQFTQALDR QTATQLLKLA HKYRPETKQE KKQRLLARAE KKAAGKGDVP TKRPPVLRAG VNTVTTLVEN KKAQLVVIAH D VDPIELVV FLPALCRKMG VPYCIIKGKA RLGRLVHRKT CTTVAFTQVN SEDKGALAKL VEAIRTNYND RYDEIRRHWG GN VLGPKSV ARIAKLEKAK AKELATKLG

UniProtKB: Large ribosomal subunit protein eL8

分子 #46: 60S ribosomal protein L8

• 分子: 名称: 60S ribosomal protein L8 / タイプ: protein_or_peptide / ID: 46 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 28.088863 KDa

配列

文字列:

MGRVIRGQRK GAGSVFRAHV KHRKGAARLR AVDFAERHGY IKGIVKDIIH DPGRGAPLAK VVFRDPYRFK KRTELFIAAE GIHTGQFVY CGKKAQLNIG NVLPVGTMPE GTIVCCLEEK PGDRGKLARA SGNYATVISH NPETKKTRVK LPSGSKKVIS S ANRAVVGV VAGGGRIDKP ILKAGRAYHK YKAKRNCWPR VRGVAMNPVE HPFGGGNHQH IGKPSTIRRD APAGRKVGLI AA RRTGRLR GTKTVQEKEN

UniProtKB: Large ribosomal subunit protein uL2

分子 #47: 60S ribosomal protein L9

• 分子: 名称: 60S ribosomal protein L9 / タイプ: protein_or_peptide / ID: 47 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 21.899471 KDa

配列

文字列:

MKTILSNQTV DIPENVDITL KGRTVIVKGP RGTLRRDFNH INVELSLLGK KKKRLRVDKW WGNRKELATV RTICSHVQNM IKGVTLGFR YKMRSVYAHF PINVVIQENG SLVEIRNFLG EKYIRRVRMR PGVACSVSQA QKDELILEGN DIELVSNSAA L IQQATTVK NKDIRKFLDG IYVSEKGTVQ QADE

UniProtKB: Large ribosomal subunit protein uL6

分子 #48: MKI67 FHA domain-interacting nucleolar phosphoprotein

• 分子: 名称: MKI67 FHA domain-interacting nucleolar phosphoprotein; タイプ: protein_or_peptide / ID: 48 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 34.285309 KDa

配列

文字列:

MATFSGPAGP ILSLNPQEDV EFQKEVAQVR KRITQRKKQE QLTPGVVYVR HLPNLLDETQ IFSYFSQFGT VTRFRLSRSK RTGNSKGYA FVEFESEDVA KIVAETMNNY LFGERLLECH FMPPEKVHKE LFKDWNIPFK QPSYPSVKRY NRNRTLTQKL R MEERFKKK ERLLRKKLAK KGIDYDFPSL ILQKTESISK TNRQTSTKGQ VLRKKKKKVS GTLDTPEKTV DSQGPTPVCT PT FLERRKS QVAELNDDDK DDEIVFKQPI SCVKEEIQET QTPTHSRKKR RRSSNQ

UniProtKB: MKI67 FHA domain-interacting nucleolar phosphoprotein

分子 #49: 60S ribosomal protein L7-like 1

• 分子: 名称: 60S ribosomal protein L7-like 1 / タイプ: protein_or_peptide / ID: 49 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 29.730354 KDa

配列

文字列:

MISSCTTRKM AEQEQRKIPL VPENLLKKRK AYQALKATQA KQALLAKKEQ KKGKGLRFKR LESFLHDSWR QKRDKVRLRR LEVKPHALE LPDKHSLAFV VRIERIDGVS LLVQRTIARL RLKKIFSGVF VKVTPQNLKM LRIVEPYVTW GFPNLKSVRE L ILKRGQAK VKNKTIPLTD NTVIEEHLGK FGVICLEDLI HEIAFPGKHF QEISWFLCPF HLSVARHATK NRVGFLKEMG TP GYRGERI NQLIRQLN

UniProtKB: Ribosomal protein uL30-like

分子 #50: Eukaryotic translation initiation factor 6

• 分子: 名称: Eukaryotic translation initiation factor 6 / タイプ: protein_or_peptide / ID: 50 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 26.62001 KDa

配列

文字列:

MAVRASFENN CEIGCFAKLT NTYCLVAIGG SENFYSVFEG ELSDTIPVVH ASIAGCRIIG RMCVGNRHGL LVPNNTTDQE LQHIRNSLP DTVQIRRVEE RLSALGNVTT CNDYVALVHP DLDRETEEIL ADVLKVEVFR QTVADQVLVG SYCVFSNQGG L VHPKTSIE DQDELSSLLQ VPLVAGTVNR GSEVIAAGMV VNDWCAFCGL DTTSTELSVV ESVFKLNEAQ PSTIATSMRD SL IDSLT

UniProtKB: Eukaryotic translation initiation factor 6

分子 #51: Pescadillo homolog

• 分子: 名称: Pescadillo homolog / タイプ: protein_or_peptide / ID: 51 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 68.114727 KDa

配列

文字列:

MGGLEKKKYE RGSATNYITR NKARKKLQLS LADFRRLCIL KGIYPHEPKH KKKVNKGSTA ARTFYLIKDI RFLLHEPIVN KFREYKVFV RKLRKAYGKS EWNTVERLKD NKPNYKLDHI IKERYPTFID ALRDLDDALS MCFLFSTFPR TGKCHVQTIQ L CRRLTVEF MHYIIAARAL RKVFLSIKGI YYQAEVLGQP IVWITPYAFS HDHPTDVDYR VMATFTEFYT TLLGFVNFRL YQ LLNLHYP PKLEGQAQAE AKAGEGTYAL DSESCMEKLA ALSASLARVV VPATEEEAEV DEFPTDGEMS AQEEDRRKEL EAQ EKHKKL FEGLKFFLNR EVPREALAFI IRSFGGEVSW DKSLCIGATY DVTDSRITHQ IVDRPGQQTS VIGRCYVQPQ WVFD SVNAR LLLPVAEYFS GVQLPPHLSP FVTEKEGDYV PPEKLKLLAL QRGEDPGNLN ESEEEEEEDD NNEGDGDEEG ENEEE EEDA EAGSEKEEEA RLAALEEQRM EGKKPRVMAG TLKLEDKQRL AQEEESEAKR LAIMMMKKRE KYLYQKIMFG KRRKIR EAN KLAEKRKAHD EAVRSEKKAK KARPE

UniProtKB: Pescadillo homolog

分子 #52: mRNA turnover protein 4 homolog

• 分子: 名称: mRNA turnover protein 4 homolog / タイプ: protein_or_peptide / ID: 52 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 27.602535 KDa

配列

文字列:

MPKSKRDKKV SLTKTAKKGL ELKQNLIEEL RKCVDTYKYL FIFSVANMRN SKLKDIRNAW KHSRMFFGKN KVMMVALGRS PSDEYKDNL HQVSKRLRGE VGLLFTNRTK EEVNEWFTKY TEMDYARAGN KAAFTVSLDP GPLEQFPHSM EPQLRQLGLP T ALKRGVVT LLSDYEVCKE GDVLTPEQAR VLKLFGYEMA EFKVTIKYMW DSQSGRFQQM GDDLPESASE STEESDSEDD D

UniProtKB: mRNA turnover protein 4 homolog

分子 #53: GTP-binding protein 4

• 分子: 名称: GTP-binding protein 4 / タイプ: protein_or_peptide / ID: 53 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 74.10782 KDa

配列

文字列:

MAHYNFKKIT VVPSAKDFID LTLSKTQRKT PTVIHKHYQI HRIRHFYMRK VKFTQQNYHD RLSQILTDFP KLDDIHPFYA DLMNILYDK DHYKLALGQI NIAKNLVDNV AKDYVRLMKY GDSLYRCKQL KRAALGRMCT VIKRQKQSLE YLEQVRQHLS R LPTIDPNT RTLLLCGYPN VGKSSFINKV TRADVDVQPY AFTTKSLFVG HMDYKYLRWQ VVDTPGILDH PLEDRNTIEM QA ITALAHL RAAVLYVMDL SEQCGHGLRE QLELFQNIRP LFINKPLIVV ANKCDVKRIA ELSEDDQKIF TDLQSEGFPV IET STLTEE GVIKVKTEAC DRLLAHRVET KMKGNKVNEV LNRLHLAIPT RRDDKERPPF IPEGVVARRK RMETEESRKK RERD LELEM GDDYILDLQK YWDLMNLSEK HDKIPEIWEG HNIADYIDPA IMKKLEELEK EEELRTAAGE YDSVSESEDE EMLEI RQLA KQIREKKKLK ILESKEKNTQ GPRMPRTAKK VQRTVLEKEM RSLGVDMDDK DDAHYAVQAR RSRSITRKRK REDSAP PSS VARSGSCSRT PRDVSGLRDV KMVKKAKTMM KNAQKKMNRL GKKGEADRHV FDMKPKHLLS GKRKAGKKDR R

UniProtKB: GTP-binding protein 4

分子 #54: Probable ribosome biogenesis protein RLP24

• 分子: 名称: Probable ribosome biogenesis protein RLP24 / タイプ: protein_or_peptide / ID: 54 / コピー数: 1 / 光学異性体: LEVO
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 19.666258 KDa

配列

文字列:

MRIEKCYFCS GPIYPGHGMM FVRNDCKVFR FCKSKCHKNF KKKRNPRKVR WTKAFRKAAG KELTVDNSFE FEKRRNEPIK YQRELWNKT IDAMKRVEEI KQKRQAKFIM NRLKKNKELQ KVQDIKEVKQ NIHLIRAPLA GKGKQLEEKM VQQLQEDVDM E DAP

UniProtKB: Probable ribosome biogenesis protein RLP24

分子 #2: 5.8S rRNA

• 分子: 名称: 5.8S rRNA / タイプ: rna / ID: 2 / コピー数: 1
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 50.46384 KDa

配列

文字列:

CGACUCUUAG CGGUGGAUCA CUCGGCUCGU GCGUCGAUGA AGAACGCAGC UAGC(PSU)GCGAG AAUUAAUG(PSU)G AA UU(OMG)CAGG ACACAUUGAU CAUCGACACU UCGAACGCAC UUGCGGCCCC GGGUUCCUCC CGGGGCUACG CCUGUCUGA GCGUCGCUU

GENBANK: GENBANK: U13369.1

分子 #3: 28S rRNA

• 分子: 名称: 28S rRNA / タイプ: rna / ID: 3 / コピー数: 1
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 1.6412037499999999 MDa

配列

文字列:

CGCGACCUCA GAUCAGACGU GGCGACCCGC UGAAUUUAAG CAUAUUAGUC AGCGGAGGAG AAGAAACUAA CCAGGAUUCC CUCAGUAAC GGCGAGUGAA CAGGGAAGAG CCCAGCGCCG AAUCCCCGCC CCGCGGCGGG GCGCGGGACA UGUGGCGUAC G GAAGACCC GCUCCCCGGC GCCGCUCGUG GGGGGCCCAA GUCCUUCUGA UCGAGGCCCA GCCCGUGGAC GGUGUGAGGC CG GUAGCGG CCCCCGGCGC GCCGGGCCCG GGUCUUCCCG GAGUCGGGUU GCUUGGGAAU GCAGCCCAAA GCGGGUGGUA AAC UCCAUC UAAGGCUAAA UACCGGCACG AGACCGAUAG UCAACAAGUA CCGUAAGGGA AAGUUGAAAA GAACUUUGAA G (A2M)G(A2M)GAGUU CAAGAGGGCG UGAAACCGUU AAGAGGUAAA CGGGUGGGGU CCGCGCAGUC CGCCCGGAGG AUUC AACCC GGCGGCGGGU CCGGCCGUGU CGGCGGCCCG GCGGAUCUUU CCCGCCCCCC GUUCCUCCCG ACCCCUCCAC CCGCC CUCC CUUCCCCCGC CGCCCCUCCU CCUCCUCCCC GGAGGGGGCG GGCUCCGGCG GGUGCGGGGG UGGGCGGGCG GGGCCG GGG GUGGGGUCGG CGGGGGACCG UCCCCCGACC GGCGACCGGC CGCCGCCGGG CGCAUUUCCA CCGCGGCGGU GCGCCGC GA CCGGCUCCGG GACGGCUGGG AAGGCCCGGC GGGGAAGGUG GCUCGGGGGG CCCCGUCCGU CCGUCCGUCC GUCCUCCU C CUCCCCCGUC UCCGCCCCCC GGCCCCGCGU CCUCCCUCGG GAGGGCGCGC GGGUCGGGGC GGCGGCGGCG GCGGCGGUG GCGGCGGCGG CGGCGGCGGC GGGACCGAAA CCCCCCCCGA GUGUUACAGC CCCCCCGGCA GCAGCACUCG CCGAAUCCCG GGGCCGAGG GAGCGAGACC CGUCGCCGCG CUCUCCCCCC UCCCGGCGCC CACCCCCGCG GGGAAUCCCC CGCGAGGGGG G UCUCCCCC GCGGGGGCGC GCCGGCGUCU CCUCGUGGGG GGGCCGGGCC ACCCCUCCCA CGGCGCGACC GCUCUCCCAC CC CUCCUCC CCGCGCCCCC GCCCCGGCGA CGGGGGGGGU GCCGCGCGCG GGUCGGGGGG CGGGGCGGAC UGUCCCCAGU GCG CCCCGG GCGGGUCGCG CCGUCGGGCC CGGGGGAGGU UCUCUCGGGG CCACGCGCGC GUCCCCCGAA GAGGGGGACG GCGG AGCGA GCGCACGGGG UCGGCGGCGA CGUCGGCUAC CCACCCGACC C(OMG)UCUUG(1MA)AA C(A2M)CGGACCAA GGA GU(OMC)UAA CACGUGCGCG AGUCGGGGGC UCGCACGAAA GCCGCCGUGG CGCAAUGAAG GUGAAGGCCG GCGCGCUCGC CGGCCGAGG UGGGAUCCCG AGGCCUCUCC AGUCCGCCGA GGGCGCACCA CCGGCCCGUC UCGCCCGCCG CGCCGGGGAG G UGGAGCAC GAGCGCACGU GUUAGGACCC (OMG)A(A2M)AGAUGGU GA(A2M)C(PSU)AUGCC UGGGCAGGGC GAAGCC AGA GGAAACUCUG GUGGAGGUCC G(PSU)AGCGGUCC UGACGUGCAA AUCGGUCGUC CGACCUGGGU AUAG(OMG)GGCG AAAGACUAAU CGAACCAUCU AGUAGCUGGU UCCCUCCGAA GUUUCCC(PSU)CA GGA(PSU)AGCUGG CGCUCUCGCA GA CCCGACG CACCCCCGCC ACGCAGUUUU AUCCGGUAAA GCGAA(PSU)GAUU AGAGGUCUUG G(OMG)GCCGAAAC GAUC (OMU)CAAC C(PSU)A(PSU)(PSU)CUCAA ACUU(PSU)AAAUG GGUAAGAAGC CCGGCUCGCU GGCGUGGAGC CGGGC GUGG AAUGCGAGUG CCUAGUGGGC CAC(PSU)U(PSU)UGGU AAGC(A2M)GAACU GGCGCUGCGG GAUGAACCGA ACGC CGGGU UAAGGCGCCC GAUGCCGACG CUCAUCAGAC CCCAGAAAAG GUGUUGGUUG AUAUAGACAG CAGGACGGUG GCCAU GGAA GUCGGAAUCC GCUAAGGAGU GUGUAACAAC UCACCUGCCG AAUCAACUAG CCCUGAAAAU GGAUGGCGCU GGAGCG UCG GGCCCAUACC CGGCCGUCGC CGGCAGUCGA GAGUGGACGG GAGCGGCGGG GGCGGCGCGC GCGCGCGCGC GUGUGGU GU GCGUCGGAGG GCGGCGGCGG CGGCGGCGGC GGGGGUGUGG GGUCCUUCCC CCGCCCCCCC CCCCACGCCU CCUCCCCU C CUCCCGCCCA CGCCCCGCUC CCCGCCCCCG GAGCCCCGCG GACGCUACGC CGCGACGAGU AGGAGGGCCG CUGCGGUGA GCCUUGAAGC CUAGGGCGCG GGCCCGGGUG GAGCCGCCGC AGGUGCAGAU (OMC)UUGGUGGUA GU(A2M)(OMG) (OMC)AAAU AUUCAAACGA GAACUUUGAA GGCCGAAGUG G(A2M)GAAGGGUU CCAUG(OMU)GAAC AG(OMC)A(OMG) UUGA ACAUGGGUCA GUCGGUCCUG AGAGAUGGGC GAGCGCCGUU CCGAAGGGAC GGGCGAUGGC CUCCGUUGCC CUCGGC CGA (PSU)CGAAAGGGA GUCGGGUUCA GAUCCCCGAA UCCGGAGUGG CGGAGAUGGG CGCCGCGAGG CGUCCAGUGC GGU AACGCG ACCGAUCCCG GAGAAGCCGG CGGGAGCCCC GGGGAGAGUU CUCUU(PSU)UCUU UGUGAAGGGC AGGGCGCCCU GGAAUGGGU UCGCCCCGAG AGAGGGGCCC GUGCCUUGGA AAGCGUCGCG GUUCCGGCGG CGUCCGGUGA GCUCUCGCUG G CCCUUGAA AAUCCGGGGG AGAGGGUGUA AAUCUCGCGC CGGGCCGUAC CC(A2M)UAUCCGC AGCAGGUCU(OMC) CAAG GUGAA C(A2M)GCCUCUGG (OMC)AUGUUGGAA CAA(OMU)G(PSU)AGGU AAGGGAAGUC GGCAAGC(OMC)GG AUCCG UAAC UUC(OMG)GGAUAA GGAUUGGCUC UAAGGGCUGG GUCGGUCGGG CUGGGGCGCG AAGCGGGGCU GGGCGCGCGC CG CGGCUGG ACGAGGCGCC GCCGCCCCCC CCACGCCCGG GGCACCCCCC UCGCGGCCCU CCCCCGCCCC ACCCCGCGCG CGC CGCUCG CUCCCUCCCC GCCCCGCGCC CUCUCUCUCU CUCUCUCCCC CGCUCCCCGU CCUCCCCCCU CCCCGGGGGA GCGC CGCGU GGGGGCGGCG GCGGGGGGAG AAGGGUCGGG GCGGCAGGGG CCGGCGGCGG CCCGCCGCGG GGCCCCGGCG GCGGG GGCA CGGUCCCCCG CGAGGGGGGC CCGGGCACCC GGGGGGCCGG CGGCGGCGGC GACUCUGGAC GCGAGCCGGG CCCUUC CCG UGGAUCGCCC CAGCUGCGGC GGGCGUCGCG GCCGCCCCCG GGGAGCCCGG CGGGCGCCGG CGCGCCCCCC CCCCCAC CC CACGUCUCGU CGCGCGCGCG UCCGCUGGGG GCGGGGAGCG GUCGGGCGGC GGCGGUCGGC GGGCGGCGGG GCGGGGCG G UUCGUCCCCC CGCCCUACCC CCCCGGCCCC GUCCGCCCCC CGUUCCCCCC UCCUCCUCGG CGCGCGGCGG CGGCGGCGG CAGGCGGCGG AGGGGCCGCG GGCCGGUCCC CCCCGCCGGG UCCGCCCCCG GGGCCGCGGU UCCGCGCGGC GCCUCGCCUC GGCCGGCGC CUAGCAGCCG ACUUAGAACU GGUGCGGACC AGG(OMG)GAAUCC GAC(PSU)G(PSU)UUAA UUAAAACAA AGCAUCGCGA AGGCCCGCGG CGGGUGUUGA CGCGAUGUGA UU(PSU)CUGCC(OMC)A GUGCUCUGAA UG(PSU)CA (A2M)AGU GA(A2M)GAAAU(PSU)C AA(PSU)GAAGCGC GG(OMG)UAAACGG CGGGAG(PSU)A(A2M)C (PSU)A (PSU)GAC(PSU)C(PSU) CUUAAGGUAG C(5MC)AA(A2M)UGCCU C(OMG)UCAUCUAA UUAGUGA(OMC)GC GCAUG AA(OMU)G GA(PSU)GA(A2M)CGAG (A2M)UUCCCACUG U(OMC)CC(PSU)ACCUA C(PSU)A(PSU)CCAGCG AAAC CAC(A2M)G (OMC)CAAGGGAAC GGGCU(PSU)GG(OMC)G GAAUCAGCGG (OMG)GAAAGAAGA CCCUGUUGAG C (PSU)UGAC(OMU)CU AGUCUGGCAC GGUGAAGAGA CAUGAGAGGU G(PSU)AGAAUAAG UGGGAGGCCC CCGGCGCCCC CCCGGUGUC CCCGCGAGGG GCCCGGGGCG GGGUCCGCCG GCCCUGCGGG CCGCC(OMG)GUGA AAUACCA(OMC)UA CUC UGAUCG UUUUUUCACU GACCCGGUGA GGCGGGGGGG CGAGCCCCGA GGGGCUCUCG CUUCUGGCGC CAAGCGCCCG GCCG CGCGC CGGCCGGGCG CGACCCGCUC CGGGGACAGU GCCAGGUGGG GAGUUUGACU G(OMG)GGCGGUAC ACCUGUCAAA C GGUA(6MZ)CGC AGG(OMU)(OMG)UCCUA AGGCGAGCUC AGGGAGGACA GAAACCUCCC GUGGAGCAGA AGGGCAAAAG CUCGCUUGA (PSU)CU(PSU)GA(PSU)UUU CAG(OMU)ACGAA(PSU) ACAGACCGUG AAAGCGGGGC CUCACGAUCC U UCUGACCU U(PSU)UGGGUUU(PSU) AAGCAGGA(OMG)G UGUCAGAAAA GUUACCACAG (OMG)GAUAACUGG C(PSU) UGUGGCG GCCAAGCGU(PSU) CA(PSU)AGCGACG (PSU)CGCUUUUUG A(PSU)CCUU(5MC)GAU GUCGG(OMC) (PSU)CU UCCUAUCAUU G(PSU)GAAGCAGA AUUCACCAAG CGU(PSU)(OMG)GAU(OMU)(OMG) (PSU)UCACCCAC UAAUAGGGAA CG(PSU)G(A2M)GCUGG G(UR3)U(PSU)AGA(OMC)CG UCGUGAGACA GGU(PSU)AGUUUU ACCCUA CUG AUG(A2M)UGUG(PSU)U G(PSU)UGCCAUGG UA(A2M)UCCUGCU CAGUACGAGA GGAACCGCAG (OMG)U(OMU) CA(OMG)ACA U(PSU)UGGUGUA(PSU) (OMG)UGCUUGGCU GAGGAGCCAA UGGGGCGAAG CUACCA(PSU)CUG UGGG AUUAU GAC(PSU)GAACGC CUCUAAGUCA GAAUCCCGCC CAGGCGGAAC GAUACGGCAG CGCCGCGGAG CCUCGGUUGG C CUCGGAUA GCCGGUCCCC CGCCUGUCCC CGCCGGCGGG CCGCCCCCCC CCUCCACGCG CCCCGCGCGC GCGGGAGGGC GC GUGCCCC GCCGCGCGCC GGGACCGGGG UCCGGUGCGG AGUGCCCUUC GUCCUGGGAA ACGGGGCGCG GCCGGAGAGG CGG CCGCCC CCUCGCCCGU CACGCACCGC ACGUUCGUGG GGAACCUGGC GCUAAACCA(PSU) UCGUAGACGA CCUGCUUCUG GGUCGGGG(PSU) UUCGUACG(PSU)A GCAGAGCAGC UCCCUCGCUG CGAUCUAUUG AAAGUCAGCC CUCGACACAA GGG UUUGUC

GENBANK: GENBANK: AL592188.60

分子 #4: 5S rRNA

• 分子: 名称: 5S rRNA / タイプ: rna / ID: 4 / コピー数: 1
• 由来(天然): 生物種: human (ヒト)
• 分子量: 理論値: 38.998078 KDa

配列

文字列:

GUCUACGGCC AUACCACCCU GAACGCGCCC GAUCUCGUCU GAUCUCGGAA GCUAAGCAGG GUCGGGCCUG GUUAGUACUU GGAUGGGAG ACCGCCUGGG AAUACCGGGU GCUGUAGGCU UU

GENBANK: GENBANK: X12811.1

分子 #55: MAGNESIUM ION

• 分子: 名称: MAGNESIUM ION / タイプ: ligand / ID: 55 / コピー数: 88 / 式: MG
• 分子量: 理論値: 24.305 Da

分子 #56: ZINC ION

• 分子: 名称: ZINC ION / タイプ: ligand / ID: 56 / コピー数: 6 / 式: ZN
• 分子量: 理論値: 65.409 Da

分子 #57: GUANOSINE-5'-DIPHOSPHATE

• 分子: 名称: GUANOSINE-5'-DIPHOSPHATE / タイプ: ligand / ID: 57 / コピー数: 1 / 式: GDP
• 分子量: 理論値: 443.201 Da

Chemical component information

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP / GDP, エネルギー貯蔵分子*YM

分子 #58: POTASSIUM ION

• 分子: 名称: POTASSIUM ION / タイプ: ligand / ID: 58 / コピー数: 1 / 式: K
• 分子量: 理論値: 39.098 Da

実験情報

構造解析

• 手法: クライオ電子顕微鏡法
• 解析: 単粒子再構成法
• 試料の集合状態: particle

試料調製

• 緩衝液: pH: 7.6
• グリッド: モデル: Quantifoil R3.5/1 / 材質: GOLD / メッシュ: 400 / 支持フィルム - 材質: CARBON / 支持フィルム - トポロジー: CONTINUOUS / 支持フィルム - Film thickness: 2 / 前処理 - タイプ: GLOW DISCHARGE / 前処理 - 時間: 30 sec.
• 凍結: 凍結剤: ETHANE / チャンバー内湿度: 95 % / チャンバー内温度: 283 K / 装置: FEI VITROBOT MARK IV; 詳細: Four applications with manual blotting before last blotting with the vitrobot..

電子顕微鏡法

• 顕微鏡: FEI TITAN KRIOS
• 特殊光学系: エネルギーフィルター - スリット幅: 20 eV
• 撮影: フィルム・検出器のモデル: GATAN K3 (6k x 4k) / 撮影したグリッド数: 4 / 実像数: 172699 / 平均露光時間: 2.0 sec. / 平均電子線量: 60.0 e/Ų
• 電子線: 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN
• 電子光学系: 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス（公称値）: 2.5 µm / 最小 デフォーカス（公称値）: 0.5 µm / 倍率（公称値）: 64000
• 実験機器: モデル: Titan Krios / 画像提供: FEI Company

画像解析

• 粒子像選択: 選択した数: 15679142
• 初期モデル: モデルのタイプ: INSILICO MODEL
• 最終 再構成: 想定した対称性 - 点群: C₁ (非対称) / 解像度のタイプ: BY AUTHOR / 解像度: 2.55 Å / 解像度の算出法: FSC 0.143 CUT-OFF / ソフトウェア - 名称: cryoSPARC (ver. 3.3.2) / 使用した粒子像数: 74556
• 初期 角度割当: タイプ: MAXIMUM LIKELIHOOD / ソフトウェア - 名称: RELION (ver. 4.0)
• 最終 角度割当: タイプ: MAXIMUM LIKELIHOOD / ソフトウェア - 名称: cryoSPARC (ver. 3.3.2)
• 最終 3次元分類: ソフトウェア: (名称: RELION (ver. 4.0), cryoSPARC (ver. 3.3.2))

原子モデル構築 1

• 精密化: 空間: REAL

得られたモデル

PDB-8fl7:
Human nuclear pre-60S ribosomal subunit (State J2)