登録情報 データベース : EMDB / ID : EMD-28933 ダウンロードとリンクタイトル Cryo-EM structure of the CapZ-capped barbed end of F-actin マップデータSharpened final map of the CapZ-bound actin filament barbed end. 詳細 試料複合体 : CapZ-capped barbed end of F-actin複合体 : CapZ (alpha and beta subunits)タンパク質・ペプチド : F-actin-capping protein subunit alpha-1タンパク質・ペプチド : F-actin-capping protein subunit beta複合体 : Actin filamentタンパク質・ペプチド : Actin, alpha skeletal muscleリガンド : ADENOSINE-5'-DIPHOSPHATEリガンド : MAGNESIUM ION 詳細 キーワード Barbed end capping protein / F-actin / CapZ / STRUCTURAL PROTEIN機能・相同性 機能・相同性情報分子機能 ドメイン・相同性 構成要素
WASH complex / : / F-actin capping protein complex / negative regulation of filopodium assembly / cell junction assembly / COPI-independent Golgi-to-ER retrograde traffic / actin polymerization or depolymerization / barbed-end actin filament capping / RHOF GTPase cycle / RHOD GTPase cycle ... WASH complex / : / F-actin capping protein complex / negative regulation of filopodium assembly / cell junction assembly / COPI-independent Golgi-to-ER retrograde traffic / actin polymerization or depolymerization / barbed-end actin filament capping / RHOF GTPase cycle / RHOD GTPase cycle / regulation of cell morphogenesis / regulation of lamellipodium assembly / Sensory processing of sound by inner hair cells of the cochlea / lamellipodium assembly / cytoskeletal motor activator activity / cortical cytoskeleton / tropomyosin binding / mesenchyme migration / troponin I binding / myosin heavy chain binding / filamentous actin / actin filament bundle / brush border / skeletal muscle thin filament assembly / striated muscle thin filament / actin filament bundle assembly / Advanced glycosylation endproduct receptor signaling / skeletal muscle myofibril / actin monomer binding / COPI-mediated anterograde transport / skeletal muscle fiber development / stress fiber / titin binding / cytoskeleton organization / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / MHC class II antigen presentation / actin filament polymerization / Gene and protein expression by JAK-STAT signaling after Interleukin-12 stimulation / sarcomere / hippocampal mossy fiber to CA3 synapse / filopodium / actin filament / 加水分解酵素; 酸無水物に作用; 酸無水物に作用・細胞または細胞小器官の運動に関与 / cell morphogenesis / Schaffer collateral - CA1 synapse / actin filament binding / calcium-dependent protein binding / actin cytoskeleton / Factors involved in megakaryocyte development and platelet production / lamellipodium / actin binding / cell body / actin cytoskeleton organization / protein-containing complex assembly / postsynaptic density / cytoskeleton / cadherin binding / hydrolase activity / protein domain specific binding / calcium ion binding / positive regulation of gene expression / magnesium ion binding / extracellular exosome / extracellular region / ATP binding / identical protein binding / membrane / cytoplasm / cytosol 類似検索 - 分子機能 F-actin-capping protein subunit beta / F-actin capping protein, beta subunit, conserved site / F-actin-capping protein subunit beta, N-terminal domain / F-actin capping protein, beta subunit / F-actin capping protein beta subunit signature. / F-actin capping protein, alpha subunit, conserved site / F-actin capping protein alpha subunit signature 1. / F-actin capping protein alpha subunit signature 2. / F-actin-capping protein subunit alpha / F-actin-capping protein subunit alpha/beta ... F-actin-capping protein subunit beta / F-actin capping protein, beta subunit, conserved site / F-actin-capping protein subunit beta, N-terminal domain / F-actin capping protein, beta subunit / F-actin capping protein beta subunit signature. / F-actin capping protein, alpha subunit, conserved site / F-actin capping protein alpha subunit signature 1. / F-actin capping protein alpha subunit signature 2. / F-actin-capping protein subunit alpha / F-actin-capping protein subunit alpha/beta / F-actin-capping protein subunit alpha/beta, domain 2 / F-actin capping protein, alpha subunit, domain 1 / F-actin capping protein alpha subunit / Actins signature 1. / Actin, conserved site / Actins signature 2. / Actin/actin-like conserved site / Actins and actin-related proteins signature. / Actin / Actin family / Actin / ATPase, nucleotide binding domain 類似検索 - ドメイン・相同性 F-actin-capping protein subunit beta / F-actin-capping protein subunit alpha-1 / Actin, alpha skeletal muscle 類似検索 - 構成要素生物種 Homo sapiens (ヒト) / Oryctolagus cuniculus (ウサギ) / rabbit (ウサギ)手法 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度 : 2.79 Å 詳細 データ登録者Carman PJ / Barrie KR / Dominguez R 資金援助 米国, 3件 詳細 詳細を隠すOrganization Grant number 国 National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) GM073791 米国 National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) GM136511 米国 National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI) HL156431 米国
引用ジャーナル : Science / 年 : 2023タイトル : Structures of the free and capped ends of the actin filament.著者 : Peter J Carman / Kyle R Barrie / Grzegorz Rebowski / Roberto Dominguez / 要旨 : The barbed and pointed ends of the actin filament (F-actin) are the sites of growth and shrinkage and the targets of capping proteins that block subunit exchange, including CapZ at the barbed end and ... The barbed and pointed ends of the actin filament (F-actin) are the sites of growth and shrinkage and the targets of capping proteins that block subunit exchange, including CapZ at the barbed end and tropomodulin at the pointed end. We describe cryo-electron microscopy structures of the free and capped ends of F-actin. Terminal subunits at the free barbed end adopt a "flat" F-actin conformation. CapZ binds with minor changes to the barbed end but with major changes to itself. By contrast, subunits at the free pointed end adopt a "twisted" monomeric actin (G-actin) conformation. Tropomodulin binding forces the second subunit into an F-actin conformation. The structures reveal how the ends differ from the middle in F-actin and how these differences control subunit addition, dissociation, capping, and interactions with end-binding proteins. 履歴 登録 2022年11月22日 - ヘッダ(付随情報) 公開 2023年6月7日 - マップ公開 2023年6月7日 - 更新 2023年7月5日 - 現状 2023年7月5日 処理サイト : RCSB / 状態 : 公開
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