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- EMDB-28074: CryoEM of the soluble OPA1 dimer from the apo helical assembly on... -

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Basic information

Entry
Database: EMDB / ID: EMD-28074
TitleCryoEM of the soluble OPA1 dimer from the apo helical assembly on a lipid membrane
Map datasOPA1 apo helical assembly full map
Sample
  • Complex: cryoEM of the soluble OPA1 helical assembly on a lipid membrane in the apo state
    • Protein or peptide: Dynamin-like 120 kDa protein, form S1
KeywordsGTPase / Dynamin-family protein / mitochondrial fusion protein / mitochondria / Optic Atrophy / LIPID BINDING PROTEIN
Function / homology
Function and homology information


Regulation of Apoptosis / membrane tubulation / inner mitochondrial membrane organization / dynamin GTPase / cardiolipin binding / mitochondrial genome maintenance / phosphatidic acid binding / mitochondrial fission / GTP metabolic process / mitochondrial fusion ...Regulation of Apoptosis / membrane tubulation / inner mitochondrial membrane organization / dynamin GTPase / cardiolipin binding / mitochondrial genome maintenance / phosphatidic acid binding / mitochondrial fission / GTP metabolic process / mitochondrial fusion / axonal transport of mitochondrion / negative regulation of release of cytochrome c from mitochondria / mitochondrial crista / negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway / axon cytoplasm / Mitochondrial protein degradation / visual perception / mitochondrion organization / neural tube closure / mitochondrial membrane / mitochondrial intermembrane space / cellular senescence / protein complex oligomerization / microtubule binding / microtubule / mitochondrial outer membrane / mitochondrial inner membrane / GTPase activity / apoptotic process / dendrite / GTP binding / negative regulation of apoptotic process / magnesium ion binding / mitochondrion / nucleoplasm / membrane / cytoplasm / cytosol
Similarity search - Function
Dynamin-like GTPase OPA1, C-terminal / Dynamin-like GTPase OPA1 C-terminal / Dynamin, GTPase domain / Dynamin, GTPase / Dynamin / Dynamin-type guanine nucleotide-binding (G) domain / Dynamin-type guanine nucleotide-binding (G) domain profile. / Dynamin, N-terminal / Dynamin family / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
Dynamin-like GTPase OPA1, mitochondrial
Similarity search - Component
Biological speciesHomo sapiens (human)
Methodhelical reconstruction / cryo EM / Resolution: 9.68 Å
AuthorsNyenhuis SB / Wu X / Stanton AE / Strub MP / Yim YI / Canagarajah B / Hinshaw JE
Funding support United States, 1 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of Diabetes and Digestive and Kidney Disease (NIH/NIDDK) United States
CitationJournal: Nature / Year: 2023
Title: OPA1 helical structures give perspective to mitochondrial dysfunction.
Authors: Sarah B Nyenhuis / Xufeng Wu / Marie-Paule Strub / Yang-In Yim / Abigail E Stanton / Valentina Baena / Zulfeqhar A Syed / Bertram Canagarajah / John A Hammer / Jenny E Hinshaw /
Abstract: Dominant optic atrophy is one of the leading causes of childhood blindness. Around 60-80% of cases are caused by mutations of the gene that encodes optic atrophy protein 1 (OPA1), a protein that has ...Dominant optic atrophy is one of the leading causes of childhood blindness. Around 60-80% of cases are caused by mutations of the gene that encodes optic atrophy protein 1 (OPA1), a protein that has a key role in inner mitochondrial membrane fusion and remodelling of cristae and is crucial for the dynamic organization and regulation of mitochondria. Mutations in OPA1 result in the dysregulation of the GTPase-mediated fusion process of the mitochondrial inner and outer membranes. Here we used cryo-electron microscopy methods to solve helical structures of OPA1 assembled on lipid membrane tubes, in the presence and absence of nucleotide. These helical assemblies organize into densely packed protein rungs with minimal inter-rung connectivity, and exhibit nucleotide-dependent dimerization of the GTPase domains-a hallmark of the dynamin superfamily of proteins. OPA1 also contains several unique secondary structures in the paddle domain that strengthen its membrane association, including membrane-inserting helices. The structural features identified in this study shed light on the effects of pathogenic point mutations on protein folding, inter-protein assembly and membrane interactions. Furthermore, mutations that disrupt the assembly interfaces and membrane binding of OPA1 cause mitochondrial fragmentation in cell-based assays, providing evidence of the biological relevance of these interactions.
History
DepositionSep 8, 2022-
Header (metadata) releaseJun 28, 2023-
Map releaseJun 28, 2023-
UpdateSep 13, 2023-
Current statusSep 13, 2023Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_28074.png

Downloads & links

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Map

FileDownload / File: emd_28074.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationsOPA1 apo helical assembly full map
Voxel sizeX=Y=Z: 1.2518 Å
Density
Contour LevelBy AUTHOR: 0.026832
Minimum - Maximum-0.055667598 - 0.10340594
Average (Standard dev.)0.0019663367 (±0.013416114)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions480480480
Spacing480480480
CellA=B=C: 600.86395 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_28074_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: sOPA1 apo helical assembly half map A

Fileemd_28074_half_map_1.map
AnnotationsOPA1 apo helical assembly half map A
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: sOPA1 apo helical assembly half map B

Fileemd_28074_half_map_2.map
AnnotationsOPA1 apo helical assembly half map B
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : cryoEM of the soluble OPA1 helical assembly on a lipid membrane i...

EntireName: cryoEM of the soluble OPA1 helical assembly on a lipid membrane in the apo state
Components
  • Complex: cryoEM of the soluble OPA1 helical assembly on a lipid membrane in the apo state
    • Protein or peptide: Dynamin-like 120 kDa protein, form S1

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Supramolecule #1: cryoEM of the soluble OPA1 helical assembly on a lipid membrane i...

SupramoleculeName: cryoEM of the soluble OPA1 helical assembly on a lipid membrane in the apo state
type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Homo sapiens (human)

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Macromolecule #1: Dynamin-like 120 kDa protein, form S1

MacromoleculeName: Dynamin-like 120 kDa protein, form S1 / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 89.262516 KDa
Recombinant expressionOrganism: Escherichia coli BL21(DE3) (bacteria)
SequenceString: ATDRGSESDK HFRKVSDKEK IDQLQEELLH TQLKYQRILE RLEKENKELR KLVLQKDDKG IHHRKLKKSL IDMYSEVLDV LSDYDASYN TQDHLPRVVV VGDQSAGKTS VLEMIAQARI FPRGSGEMMT RSPVKVTLSE GPHHVALFKD SSREFDLTKE E DLAALRHE ...String:
ATDRGSESDK HFRKVSDKEK IDQLQEELLH TQLKYQRILE RLEKENKELR KLVLQKDDKG IHHRKLKKSL IDMYSEVLDV LSDYDASYN TQDHLPRVVV VGDQSAGKTS VLEMIAQARI FPRGSGEMMT RSPVKVTLSE GPHHVALFKD SSREFDLTKE E DLAALRHE IELRMRKNVK EGCTVSPETI SLNVKGPGLQ RMVLVDLPGV INTVTSGMAP DTKETIFSIS KAYMQNPNAI IL CIQDGSV DAERSIVTDL VSQMDPHGRR TIFVLTKVDL AEKNVASPSR IQQIIEGKLF PMKALGYFAV VTGKGNSSES IEA IREYEE EFFQNSKLLK TSMLKAHQVT TRNLSLAVSD CFWKMVRESV EQQADSFKAT RFNLETEWKN NYPRLRELDR NELF EKAKN EILDEVISLS QVTPKHWEEI LQQSLWERVS THVIENIYLP AAQTMNSGTF NTTVDIKLKQ WTDKQLPNKA VEVAW ETLQ EEFSRFMTEP KGKEHDDIFD KLKEAVKEES IKRHKWNDFA EDSLRVIQHN ALEDRSISDK QQWDAAIYFM EEALQA RLK DTENAIENMV GPDWKKRWLY WKNRTQEQCV HNETKNELEK MLKCNEEHPA YLASDEITTV RKNLESRGVE VDPSLIK DT WHQVYRRHFL KTALNHCNLC RRGFYYYQRH FVDSELECND VVLFWRIQRM LAITANTLRQ QLTNTEVRRL EKNVKEVL E DFAEDGEKKI KLLTGKRVQL AEDLKKVREI QEKLDAFIEA LHQEK

UniProtKB: Dynamin-like GTPase OPA1, mitochondrial

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Experimental details

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Structure determination

Methodcryo EM
Processinghelical reconstruction
Aggregation statehelical array

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Sample preparation

BufferpH: 7.2
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS GLACIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 24.86 e/Å2
Electron beamAcceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.4 µm / Nominal defocus min: 0.6 µm

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Image processing

Final reconstructionApplied symmetry - Helical parameters - Δz: 14.07 Å
Applied symmetry - Helical parameters - Δ&Phi: 37.25 °
Applied symmetry - Helical parameters - Axial symmetry: C1 (asymmetric)
Resolution.type: BY AUTHOR / Resolution: 9.68 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 88155
Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

6jtg

Final angle assignmentType: NOT APPLICABLE
FSC plot (resolution estimation)

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Atomic model buiding 1

RefinementSpace: REAL / Protocol: FLEXIBLE FIT
Output model

PDB-8ef7:
CryoEM of the soluble OPA1 dimer from the apo helical assembly on a lipid membrane

PDB-8efs:
CryoEM of the soluble OPA1 tetramer from the apo helical assembly on a lipid membrane

PDB-8eft:
CryoEM of the soluble OPA1 interfaces from the apo helical assembly on a lipid membrane

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