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基本情報
登録情報 | ![]() | |||||||||
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タイトル | Mediator-PIC Early (Mediator A) | |||||||||
![]() | Mediator-PIC Early (Mediator A) | |||||||||
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![]() | Divergent transcription / Mediator / RNA Polymerase II / PIC / Pre-Initiation / Activation / Transcription Factor / Gal4 / Gal4VP16 / TRANSCRIPTION | |||||||||
機能・相同性 | ![]() meiotic gene conversion / RNA polymerase II complex recruiting activity / core mediator complex / negative regulation of ribosomal protein gene transcription by RNA polymerase II / transcription open complex formation at RNA polymerase II promoter / TFIIH-class transcription factor complex binding / mediator complex / Processing of Capped Intron-Containing Pre-mRNA / RNA Pol II CTD phosphorylation and interaction with CE / Formation of the Early Elongation Complex ...meiotic gene conversion / RNA polymerase II complex recruiting activity / core mediator complex / negative regulation of ribosomal protein gene transcription by RNA polymerase II / transcription open complex formation at RNA polymerase II promoter / TFIIH-class transcription factor complex binding / mediator complex / Processing of Capped Intron-Containing Pre-mRNA / RNA Pol II CTD phosphorylation and interaction with CE / Formation of the Early Elongation Complex / mRNA Capping / RNA polymerase II transcribes snRNA genes / TP53 Regulates Transcription of DNA Repair Genes / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Initiation And Promoter Clearance / termination of RNA polymerase II transcription / RNA-templated transcription / RNA Polymerase II Pre-transcription Events / Formation of TC-NER Pre-Incision Complex / Gap-filling DNA repair synthesis and ligation in TC-NER / TFIID-class transcription factor complex binding / Estrogen-dependent gene expression / TFIIB-class transcription factor binding / Dual incision in TC-NER / positive regulation of transcription initiation by RNA polymerase II / RNA polymerase II core promoter sequence-specific DNA binding / RNA polymerase II, core complex / translesion synthesis / RNA polymerase II activity / RNA polymerase II preinitiation complex assembly / TBP-class protein binding / transcription elongation by RNA polymerase II / transcription initiation at RNA polymerase II promoter / positive regulation of transcription elongation by RNA polymerase II / transcription coregulator activity / cytoplasmic stress granule / DNA-directed RNA polymerase / transcription corepressor activity / protein-macromolecule adaptor activity / DNA recombination / DNA-binding transcription factor binding / RNA polymerase II-specific DNA-binding transcription factor binding / transcription by RNA polymerase II / transcription coactivator activity / RNA polymerase II cis-regulatory region sequence-specific DNA binding / protein domain specific binding / DNA repair / regulation of transcription by RNA polymerase II / structural molecule activity / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / mitochondrion / DNA binding / nucleus / metal ion binding / cytosol 類似検索 - 分子機能 | |||||||||
生物種 | ![]() ![]() | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 4.3 Å | |||||||||
![]() | Gorbea Colon JJ / Chen S-F / Tsai KL / Murakami K | |||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Structural basis of a transcription pre-initiation complex on a divergent promoter. 著者: Jose J Gorbea Colón / Leon Palao / Shin-Fu Chen / Hee Jong Kim / Laura Snyder / Yi-Wei Chang / Kuang-Lei Tsai / Kenji Murakami / ![]() 要旨: Most eukaryotic promoter regions are divergently transcribed. As the RNA polymerase II pre-initiation complex (PIC) is intrinsically asymmetric and responsible for transcription in a single ...Most eukaryotic promoter regions are divergently transcribed. As the RNA polymerase II pre-initiation complex (PIC) is intrinsically asymmetric and responsible for transcription in a single direction, it is unknown how divergent transcription arises. Here, the Saccharomyces cerevisiae Mediator complexed with a PIC (Med-PIC) was assembled on a divergent promoter and analyzed by cryoelectron microscopy. The structure reveals two distinct Med-PICs forming a dimer through the Mediator tail module, induced by a homodimeric activator protein localized near the dimerization interface. The tail dimer is associated with ∼80-bp upstream DNA, such that two flanking core promoter regions are positioned and oriented in a suitable form for PIC assembly in opposite directions. Also, cryoelectron tomography visualized the progress of the PIC assembly on the two core promoter regions, providing direct evidence for the role of the Med-PIC dimer in divergent transcription. | |||||||||
履歴 |
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構造の表示
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マップデータ | ![]() | 139.1 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 42.6 KB 42.6 KB | 表示 表示 | ![]() |
FSC (解像度算出) | ![]() | 12.8 KB | 表示 | ![]() |
画像 | ![]() | 31.7 KB | ||
Filedesc metadata | ![]() | 10.2 KB | ||
その他 | ![]() ![]() ![]() ![]() ![]() | 8.1 MB 544.8 KB 166 MB 140.5 MB 140.5 MB | ||
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-検証レポート
文書・要旨 | ![]() | 761.7 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 761.3 KB | 表示 | |
XML形式データ | ![]() | 19.7 KB | 表示 | |
CIF形式データ | ![]() | 25.9 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 7uigMC ![]() 7ui9C ![]() 7uicC ![]() 7uifC ![]() 7uikC ![]() 7uilC ![]() 7uioC M: このマップから作成された原子モデル C: 同じ文献を引用 ( |
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類似構造データ | 類似検索 - 機能・相同性 ![]() |
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||
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注釈 | Mediator-PIC Early (Mediator A) | ||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.38 Å | ||||||||||||||||||||
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対称性 | 空間群: 1 | ||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
-追加マップ: Additional map 1
ファイル | emd_26545_additional_1.map | ||||||||||||
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注釈 | Additional map 1 | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-追加マップ: Additional map 2
ファイル | emd_26545_additional_2.map | ||||||||||||
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注釈 | Additional map 2 | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-追加マップ: Additional map 3
ファイル | emd_26545_additional_3.map | ||||||||||||
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注釈 | Additional map 3 | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: Half Map 1
ファイル | emd_26545_half_map_1.map | ||||||||||||
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注釈 | Half Map 1 | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: Half Map 2
ファイル | emd_26545_half_map_2.map | ||||||||||||
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注釈 | Half Map 2 | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
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試料の構成要素
+全体 : Core Mediator-PICearly (Copy A)
+超分子 #1: Core Mediator-PICearly (Copy A)
+分子 #1: Mediator of RNA polymerase II transcription subunit 1
+分子 #2: Mediator of RNA polymerase II transcription subunit 4
+分子 #3: Mediator of RNA polymerase II transcription subunit 6
+分子 #4: Mediator of RNA polymerase II transcription subunit 7
+分子 #5: Mediator of RNA polymerase II transcription subunit 8
+分子 #6: Mediator of RNA polymerase II transcription subunit 9
+分子 #7: Mediator of RNA polymerase II transcription subunit 10
+分子 #8: Mediator of RNA polymerase II transcription subunit 11
+分子 #9: Mediator of RNA polymerase II transcription subunit 14
+分子 #10: Mediator of RNA polymerase II transcription subunit 17
+分子 #11: Mediator of RNA polymerase II transcription subunit 18
+分子 #12: Mediator of RNA polymerase II transcription subunit 19
+分子 #13: Mediator of RNA polymerase II transcription subunit 20
+分子 #14: Mediator of RNA polymerase II transcription subunit 21
+分子 #15: Mediator of RNA polymerase II transcription subunit 22
+分子 #16: Mediator of RNA polymerase II transcription subunit 31
+分子 #17: DNA-directed RNA polymerase II subunit RPB1
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
濃度 | .1 mg/mL | |||||||||||||||
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緩衝液 | pH: 7.6 構成要素:
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凍結 | 凍結剤: ETHANE / 装置: LEICA EM CPC |
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電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: GATAN K3 (6k x 4k) / 実像数: 40900 / 平均露光時間: 3.4 sec. / 平均電子線量: 42.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 最大 デフォーカス(補正後): 3.0 µm / 最小 デフォーカス(補正後): 0.5 µm / 倍率(補正後): 64000 / 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / Cs: 2.7 mm / 最大 デフォーカス(公称値): 3.0 µm / 最小 デフォーカス(公称値): 0.5 µm / 倍率(公称値): 64000 |
試料ステージ | 試料ホルダーモデル: FEI TITAN KRIOS AUTOGRID HOLDER ホルダー冷却材: NITROGEN |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |