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データを開く
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基本情報
登録情報 | ![]() | |||||||||
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タイトル | Core Mediator-PICearly (Copy A) | |||||||||
![]() | Core Mediator-PICearly (Copy A) - Unsharpened | |||||||||
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![]() | Divergent transcription / Mediator / RNA Polymerase II / PIC / Pre-Initiation / Activation / Transcription Factor / Gal4 / Gal4VP16 / TRANSCRIPTION | |||||||||
機能・相同性 | ![]() meiotic gene conversion / RNA polymerase II complex recruiting activity / transcription open complex formation at RNA polymerase II promoter / regulation of mRNA 3'-end processing / TFIIH-class transcription factor complex binding / core mediator complex / negative regulation of ribosomal protein gene transcription by RNA polymerase II / TFIIF-class transcription factor complex binding / transcriptional start site selection at RNA polymerase II promoter / RPB4-RPB7 complex ...meiotic gene conversion / RNA polymerase II complex recruiting activity / transcription open complex formation at RNA polymerase II promoter / regulation of mRNA 3'-end processing / TFIIH-class transcription factor complex binding / core mediator complex / negative regulation of ribosomal protein gene transcription by RNA polymerase II / TFIIF-class transcription factor complex binding / transcriptional start site selection at RNA polymerase II promoter / RPB4-RPB7 complex / transcription factor TFIIF complex / mediator complex / nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / RNA Polymerase I Transcription Initiation / transcription preinitiation complex / Processing of Capped Intron-Containing Pre-mRNA / RNA Polymerase III Transcription Initiation From Type 2 Promoter / RNA Pol II CTD phosphorylation and interaction with CE / Formation of the Early Elongation Complex / mRNA Capping / RNA polymerase II transcribes snRNA genes / termination of RNA polymerase II transcription / TP53 Regulates Transcription of DNA Repair Genes / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Initiation And Promoter Clearance / termination of RNA polymerase III transcription / RNA polymerase II general transcription initiation factor activity / RNA Polymerase II Pre-transcription Events / RNA-templated transcription / positive regulation of nuclear-transcribed mRNA poly(A) tail shortening / Formation of TC-NER Pre-Incision Complex / RNA Polymerase I Promoter Escape / transcription initiation at RNA polymerase III promoter / termination of RNA polymerase I transcription / nucleolar large rRNA transcription by RNA polymerase I / Gap-filling DNA repair synthesis and ligation in TC-NER / RNA polymerase II complex binding / transcription initiation at RNA polymerase I promoter / Estrogen-dependent gene expression / TFIID-class transcription factor complex binding / maintenance of transcriptional fidelity during transcription elongation by RNA polymerase II / TFIIB-class transcription factor binding / nuclear-transcribed mRNA catabolic process / positive regulation of translational initiation / protein phosphatase activator activity / Dual incision in TC-NER / positive regulation of RNA polymerase II transcription preinitiation complex assembly / positive regulation of transcription initiation by RNA polymerase II / cellular response to nutrient levels / transcription by RNA polymerase III / transcription by RNA polymerase I / translesion synthesis / RNA polymerase I complex / transcription elongation by RNA polymerase I / RNA polymerase III complex / transcription-coupled nucleotide-excision repair / RNA polymerase II core promoter sequence-specific DNA binding / RNA polymerase II, core complex / tRNA transcription by RNA polymerase III / RNA polymerase II preinitiation complex assembly / : / translation initiation factor binding / TBP-class protein binding / DNA-directed RNA polymerase activity / transcription antitermination / RNA polymerase II transcription regulatory region sequence-specific DNA binding / positive regulation of transcription elongation by RNA polymerase II / transcription coregulator activity / transcription initiation at RNA polymerase II promoter / DNA-templated transcription initiation / transcription elongation by RNA polymerase II / P-body / ribonucleoside binding / : / : / : / : / : / : / DNA-directed RNA polymerase / mRNA processing / cytoplasmic stress granule / transcription corepressor activity / peroxisome / ribosome biogenesis / single-stranded DNA binding / cellular response to heat / protein-macromolecule adaptor activity / DNA-binding transcription factor binding / DNA recombination / RNA polymerase II-specific DNA-binding transcription factor binding / transcription by RNA polymerase II / nucleic acid binding / transcription coactivator activity / single-stranded RNA binding / protein dimerization activity / protein domain specific binding / RNA polymerase II cis-regulatory region sequence-specific DNA binding 類似検索 - 分子機能 | |||||||||
生物種 | ![]() ![]() | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.3 Å | |||||||||
![]() | Gorbea Colon JJ / Chen S-F / Tsai KL / Murakami K | |||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Structural basis of a transcription pre-initiation complex on a divergent promoter. 著者: Jose J Gorbea Colón / Leon Palao / Shin-Fu Chen / Hee Jong Kim / Laura Snyder / Yi-Wei Chang / Kuang-Lei Tsai / Kenji Murakami / ![]() 要旨: Most eukaryotic promoter regions are divergently transcribed. As the RNA polymerase II pre-initiation complex (PIC) is intrinsically asymmetric and responsible for transcription in a single ...Most eukaryotic promoter regions are divergently transcribed. As the RNA polymerase II pre-initiation complex (PIC) is intrinsically asymmetric and responsible for transcription in a single direction, it is unknown how divergent transcription arises. Here, the Saccharomyces cerevisiae Mediator complexed with a PIC (Med-PIC) was assembled on a divergent promoter and analyzed by cryoelectron microscopy. The structure reveals two distinct Med-PICs forming a dimer through the Mediator tail module, induced by a homodimeric activator protein localized near the dimerization interface. The tail dimer is associated with ∼80-bp upstream DNA, such that two flanking core promoter regions are positioned and oriented in a suitable form for PIC assembly in opposite directions. Also, cryoelectron tomography visualized the progress of the PIC assembly on the two core promoter regions, providing direct evidence for the role of the Med-PIC dimer in divergent transcription. | |||||||||
履歴 |
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構造の表示
添付画像 |
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ダウンロードとリンク
-EMDBアーカイブ
マップデータ | ![]() | 141 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 61.3 KB 61.3 KB | 表示 表示 | ![]() |
FSC (解像度算出) | ![]() | 12.7 KB | 表示 | ![]() |
画像 | ![]() | 41.7 KB | ||
マスクデータ | ![]() | 178 MB | ![]() | |
Filedesc metadata | ![]() | 14.3 KB | ||
その他 | ![]() ![]() ![]() ![]() | 10.8 MB 165.1 MB 140.9 MB 140.8 MB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 7ui9MC ![]() 7uicC ![]() 7uifC ![]() 7uigC ![]() 7uikC ![]() 7uilC ![]() 7uioC M: このマップから作成された原子モデル C: 同じ文献を引用 ( |
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類似構造データ | 類似検索 - 機能・相同性 ![]() |
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||||||||||||||||||
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注釈 | Core Mediator-PICearly (Copy A) - Unsharpened | ||||||||||||||||||||||||||||||||||||
投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.38 Å | ||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
-マスク #1
ファイル | ![]() | ||||||||||||
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投影像・断面図 |
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密度ヒストグラム |
-追加マップ: Core Mediator-PICearly (Copy A) - Sharpened (DeepEMhancer)
ファイル | emd_26542_additional_1.map | ||||||||||||
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注釈 | Core Mediator-PICearly (Copy A) - Sharpened (DeepEMhancer) | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-追加マップ: Core Mediator-PICearly (Copy A) - Sharpened (Relion)
ファイル | emd_26542_additional_2.map | ||||||||||||
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注釈 | Core Mediator-PICearly (Copy A) - Sharpened (Relion) | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: Core Mediator-PICearly (Copy A) - Half Map 1
ファイル | emd_26542_half_map_1.map | ||||||||||||
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注釈 | Core Mediator-PICearly (Copy A) - Half Map 1 | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: Core Mediator-PICearly (Copy A) - Half Map 2
ファイル | emd_26542_half_map_2.map | ||||||||||||
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注釈 | Core Mediator-PICearly (Copy A) - Half Map 2 | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
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試料の構成要素
+全体 : Core Mediator-PICearly (Copy A)
+超分子 #1: Core Mediator-PICearly (Copy A)
+分子 #1: Transcription initiation factor IIB
+分子 #2: Transcription initiation factor IIF subunit alpha
+分子 #3: Transcription initiation factor IIF subunit beta
+分子 #4: Transcription elongation factor S-II
+分子 #5: Mediator of RNA polymerase II transcription subunit 1
+分子 #6: Mediator of RNA polymerase II transcription subunit 4
+分子 #7: Mediator of RNA polymerase II transcription subunit 6
+分子 #8: Mediator of RNA polymerase II transcription subunit 7
+分子 #9: Mediator of RNA polymerase II transcription subunit 8
+分子 #10: Mediator of RNA polymerase II transcription subunit 9
+分子 #11: Mediator of RNA polymerase II transcription subunit 10
+分子 #12: Mediator of RNA polymerase II transcription subunit 11
+分子 #13: Mediator of RNA polymerase II transcription subunit 14
+分子 #14: Mediator of RNA polymerase II transcription subunit 17
+分子 #15: Mediator of RNA polymerase II transcription subunit 18
+分子 #16: Mediator of RNA polymerase II transcription subunit 19
+分子 #17: Mediator of RNA polymerase II transcription subunit 20
+分子 #18: Mediator of RNA polymerase II transcription subunit 21
+分子 #19: Mediator of RNA polymerase II transcription subunit 22
+分子 #20: Mediator of RNA polymerase II transcription subunit 31
+分子 #21: DNA-directed RNA polymerase II subunit RPB1
+分子 #22: DNA-directed RNA polymerase II subunit RPB1
+分子 #23: DNA-directed RNA polymerase II subunit RPB2
+分子 #24: DNA-directed RNA polymerase II subunit RPB3
+分子 #25: DNA-directed RNA polymerase II subunit RPB4
+分子 #26: DNA-directed RNA polymerases I, II, and III subunit RPABC1
+分子 #27: DNA-directed RNA polymerases I, II, and III subunit RPABC2
+分子 #28: DNA-directed RNA polymerase II subunit RPB7
+分子 #29: DNA-directed RNA polymerases I, II, and III subunit RPABC3
+分子 #30: DNA-directed RNA polymerase II subunit RPB9
+分子 #31: DNA-directed RNA polymerases I, II, and III subunit RPABC5
+分子 #32: DNA-directed RNA polymerase II subunit RPB11
+分子 #33: DNA-directed RNA polymerases I, II, and III subunit RPABC4
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
濃度 | .1 mg/mL | |||||||||||||||
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緩衝液 | pH: 7.6 構成要素:
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グリッド | モデル: Quantifoil / 材質: COPPER / 前処理 - タイプ: GLOW DISCHARGE / 前処理 - 時間: 60 sec. | |||||||||||||||
凍結 | 凍結剤: ETHANE / 装置: LEICA EM CPC |
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電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: GATAN K3 (6k x 4k) / 実像数: 40900 / 平均露光時間: 3.4 sec. / 平均電子線量: 42.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 最大 デフォーカス(補正後): 3.0 µm / 最小 デフォーカス(補正後): 0.5 µm / 倍率(補正後): 64000 / 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / Cs: 2.7 mm / 最大 デフォーカス(公称値): 3.0 µm / 最小 デフォーカス(公称値): 0.5 µm / 倍率(公称値): 64000 |
試料ステージ | 試料ホルダーモデル: FEI TITAN KRIOS AUTOGRID HOLDER ホルダー冷却材: NITROGEN |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |