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- EMDB-26551: Mediator-PIC Early (Composite Model) -

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Basic information

Entry
Database: EMDB / ID: EMD-26551
TitleMediator-PIC Early (Composite Model)
Map datacomposite map of Med-PICearly
Sample
  • Complex: Med-PICearly
    • DNA: x 2 types
    • Protein or peptide: x 39 types
  • Ligand: x 4 types
KeywordsDivergent transcription / Mediator / RNA Polymerase II / PIC / Pre-Initiation / Activation / Transcription Factor / Gal4 / Gal4VP16 / TRANSCRIPTION
Function / homology
Function and homology information


TFIIE-class transcription factor complex binding / regulation of establishment of protein localization to chromosome / regulation of transcription from RNA polymerase II promoter by galactose / meiotic gene conversion / positive regulation of invasive growth in response to glucose limitation / RNA polymerase II complex recruiting activity / transcription open complex formation at RNA polymerase II promoter / regulation of mRNA 3'-end processing / TFIIH-class transcription factor complex binding / galactose metabolic process ...TFIIE-class transcription factor complex binding / regulation of establishment of protein localization to chromosome / regulation of transcription from RNA polymerase II promoter by galactose / meiotic gene conversion / positive regulation of invasive growth in response to glucose limitation / RNA polymerase II complex recruiting activity / transcription open complex formation at RNA polymerase II promoter / regulation of mRNA 3'-end processing / TFIIH-class transcription factor complex binding / galactose metabolic process / core mediator complex / negative regulation of ribosomal protein gene transcription by RNA polymerase II / TFIIF-class transcription factor complex binding / transcriptional start site selection at RNA polymerase II promoter / transcription factor TFIIF complex / RPB4-RPB7 complex / mediator complex / nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / transcription preinitiation complex / RNA Polymerase I Transcription Initiation / Processing of Capped Intron-Containing Pre-mRNA / RNA Polymerase III Transcription Initiation From Type 2 Promoter / RNA Pol II CTD phosphorylation and interaction with CE / Formation of the Early Elongation Complex / mRNA Capping / RNA polymerase II transcribes snRNA genes / termination of RNA polymerase II transcription / RNA polymerase II general transcription initiation factor activity / TP53 Regulates Transcription of DNA Repair Genes / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Initiation And Promoter Clearance / termination of RNA polymerase III transcription / RNA Polymerase II Pre-transcription Events / RNA-templated transcription / positive regulation of nuclear-transcribed mRNA poly(A) tail shortening / Formation of TC-NER Pre-Incision Complex / transcription initiation at RNA polymerase III promoter / RNA Polymerase I Promoter Escape / termination of RNA polymerase I transcription / transcription initiation at RNA polymerase I promoter / nucleolar large rRNA transcription by RNA polymerase I / Gap-filling DNA repair synthesis and ligation in TC-NER / RNA polymerase II complex binding / TFIID-class transcription factor complex binding / TFIIB-class transcription factor binding / Estrogen-dependent gene expression / maintenance of transcriptional fidelity during transcription elongation by RNA polymerase II / nuclear-transcribed mRNA catabolic process / positive regulation of translational initiation / transcription by RNA polymerase III / protein phosphatase activator activity / replicative senescence / Dual incision in TC-NER / positive regulation of RNA polymerase II transcription preinitiation complex assembly / positive regulation of transcription initiation by RNA polymerase II / translesion synthesis / RNA polymerase I complex / transcription elongation by RNA polymerase I / RNA polymerase III complex / cellular response to nutrient levels / RNA polymerase II core promoter sequence-specific DNA binding / RNA polymerase II preinitiation complex assembly / RNA polymerase II, core complex / tRNA transcription by RNA polymerase III / transcription by RNA polymerase I / translation initiation factor binding / transcription-coupled nucleotide-excision repair / transcription repressor complex / TBP-class protein binding / response to endoplasmic reticulum stress / transcription coregulator activity / RNA polymerase II transcription regulatory region sequence-specific DNA binding / transcription antitermination / transcription initiation at RNA polymerase II promoter / transcription elongation by RNA polymerase II / P-body / DNA-templated transcription initiation / positive regulation of transcription elongation by RNA polymerase II / ribonucleoside binding / mRNA processing / DNA-directed RNA polymerase / cytoplasmic stress granule / DNA-directed RNA polymerase activity / transcription corepressor activity / peroxisome / single-stranded DNA binding / ribosome biogenesis / cellular response to heat / DNA-binding transcription activator activity, RNA polymerase II-specific / protein-macromolecule adaptor activity / DNA recombination / DNA-binding transcription factor binding / nucleic acid binding / RNA polymerase II-specific DNA-binding transcription factor binding / transcription by RNA polymerase II / DNA-binding transcription factor activity, RNA polymerase II-specific / transcription coactivator activity / single-stranded RNA binding
Similarity search - Function
Mediator of RNA polymerase II transcription subunit 3 / Mediator complex, subunit Med2, fungi / Mediator complex subunit 2 / Mediator complex subunit 3 fungal / Mediator complex, subunit Med5, fungi / Mediator complex subunit Med5 / Gal4 dimerisation domain / Gal4-like dimerisation domain / Mediator complex, subunit Med15, fungi / Gal11, coactivator domain ...Mediator of RNA polymerase II transcription subunit 3 / Mediator complex, subunit Med2, fungi / Mediator complex subunit 2 / Mediator complex subunit 3 fungal / Mediator complex, subunit Med5, fungi / Mediator complex subunit Med5 / Gal4 dimerisation domain / Gal4-like dimerisation domain / Mediator complex, subunit Med15, fungi / Gal11, coactivator domain / Mediator complex subunit 15, KIX domain / Mediator complex subunit 15 / KIX domain / Gal11 activator-binding domain (ABD1) / : / Mediator complex, subunit Med19, fungi / Fungal specific transcription factor domain / Rox3 mediator complex subunit / Transcription factor domain, fungi / Fungal specific transcription factor domain / Mediator of RNA polymerase II transcription subunit 22, Saccharomycetes / Mediator of RNA polymerase II transcription subunit 20 / Mediator complex, subunit Med6, fungi / : / Mediator complex subunit 16, C-terminal / Mediator complex, subunit Med16 / : / Mediator complex subunit 16, N-terminal / Mediator complex, subunit Med10 / Transcription factor subunit Med10 of Mediator complex / Mediator of RNA polymerase II transcription subunit 9 / RNA polymerase II transcription mediator complex subunit 9 / Mediator complex, subunit Med4 / Vitamin-D-receptor interacting Mediator subunit 4 / Mediator complex, subunit Med31 / Mediator complex, subunit Med21 / Mediator of RNA polymerase II, subunit Med31 superfamily / SOH1 / Subunit 21 of Mediator complex / Mediator complex, subunit Med7 / Mediator complex, subunit Med7/Med21-like / Mediator complex, subunit Med7 superfmaily / : / MED7 protein / Mediator complex, subunit Med20 / Mediator complex, subunit Med14 / TATA-binding related factor (TRF) of subunit 20 of Mediator complex / Mediator complex subunit MED14 / Mediator complex, subunit Med6 / Mediator complex, subunit Med17 / Mediator complex, subunit Med6 superfamily / MED6 mediator sub complex component / Subunit 17 of Mediator complex / Zn(2)-C6 fungal-type DNA-binding domain signature. / Mediator of RNA polymerase II transcription subunit 22 / Mediator complex, subunit Med8, fungi/metazoa / Mediator complex, subunit Med11 / Surfeit locus protein 5 subunit 22 of Mediator complex / Mediator of RNA polymerase II transcription complex subunit 8 / Mediator complex protein / Mediator complex, subunit Med18 / Med18 protein / Transcription elongation factor, TFIIS / Transcription elongation factor, IIS-type / Fungal Zn(2)-Cys(6) binuclear cluster domain / Zn(2)-C6 fungal-type DNA-binding domain superfamily / Zn(2)-C6 fungal-type DNA-binding domain profile. / GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain / Zn(2)-C6 fungal-type DNA-binding domain / Transcription factor S-II (TFIIS), central domain / Domain in the central regions of transcription elongation factor S-II (and elsewhere) / Transcription elongation factor S-II, central domain / Transcription elongation factor S-II, central domain superfamily / TFIIS central domain profile. / Transcription elongation factor, TFIIS/CRSP70, N-terminal, sub-type / Domain in the N-terminus of transcription elongation factor S-II (and elsewhere) / Mediator complex, subunit Med1 / Mediator of RNA polymerase II transcription subunit 1 / TFIIS N-terminal domain profile. / Transcription factor IIS, N-terminal / TFIIS helical bundle-like domain / Transcription initiation factor IIF, beta subunit / TFIIF beta subunit, HTH domain / TFIIF, beta subunit, N-terminal / TFIIF, beta subunit HTH domain / TFIIF, beta subunit N-terminus / Transcription initiation factor IIF, alpha subunit / Transcription initiation factor IIF, alpha subunit (TFIIF-alpha) / Transcription Factor IIF, Rap30/Rap74, interaction / Transcription factor TFIIB, cyclin-like domain / Transcription factor TFIIB, conserved site / Transcription factor TFIIB repeat / Transcription factor TFIIB repeat signature. / Zinc finger TFIIB-type profile. / Transcription factor TFIIB / Zinc finger, TFIIB-type / TFIIB zinc-binding / Coactivator CBP, KIX domain superfamily / TFIIS/LEDGF domain superfamily / Basic-leucine zipper domain superfamily
Similarity search - Domain/homology
DNA-directed RNA polymerase II subunit RPB1 / Regulatory protein GAL4 / Transcription elongation factor S-II / DNA-directed RNA polymerase II subunit RPB2 / DNA-directed RNA polymerase II subunit RPB3 / Mediator of RNA polymerase II transcription subunit 14 / Mediator of RNA polymerase II transcription subunit 15 / DNA-directed RNA polymerase II subunit RPB4 / DNA-directed RNA polymerases I, II, and III subunit RPABC1 / DNA-directed RNA polymerases I, II, and III subunit RPABC2 ...DNA-directed RNA polymerase II subunit RPB1 / Regulatory protein GAL4 / Transcription elongation factor S-II / DNA-directed RNA polymerase II subunit RPB2 / DNA-directed RNA polymerase II subunit RPB3 / Mediator of RNA polymerase II transcription subunit 14 / Mediator of RNA polymerase II transcription subunit 15 / DNA-directed RNA polymerase II subunit RPB4 / DNA-directed RNA polymerases I, II, and III subunit RPABC1 / DNA-directed RNA polymerases I, II, and III subunit RPABC2 / DNA-directed RNA polymerases I, II, and III subunit RPABC3 / DNA-directed RNA polymerases I, II, and III subunit RPABC5 / Mediator of RNA polymerase II transcription subunit 19 / DNA-directed RNA polymerase II subunit RPB9 / Transcription initiation factor IIB / Mediator of RNA polymerase II transcription subunit 16 / Mediator of RNA polymerase II transcription subunit 17 / Mediator of RNA polymerase II transcription subunit 22 / Mediator of RNA polymerase II transcription subunit 18 / Mediator of RNA polymerase II transcription subunit 9 / DNA-directed RNA polymerase II subunit RPB7 / Mediator of RNA polymerase II transcription subunit 20 / Mediator of RNA polymerase II transcription subunit 8 / Mediator of RNA polymerase II transcription subunit 31 / Mediator of RNA polymerase II transcription subunit 6 / DNA-directed RNA polymerase II subunit RPB11 / Mediator of RNA polymerase II transcription subunit 3 / DNA-directed RNA polymerases I, II, and III subunit RPABC4 / Transcription initiation factor IIF subunit alpha / Transcription initiation factor IIF subunit beta / Mediator of RNA polymerase II transcription subunit 21 / Mediator of RNA polymerase II transcription subunit 5 / Mediator of RNA polymerase II transcription subunit 10 / Mediator of RNA polymerase II transcription subunit 7 / Mediator of RNA polymerase II transcription subunit 2 / Mediator of RNA polymerase II transcription subunit 1 / Mediator of RNA polymerase II transcription subunit 4 / Mediator of RNA polymerase II transcription subunit 11
Similarity search - Component
Biological speciesSaccharomyces cerevisiae S288C (yeast)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.3 Å
AuthorsGorbea Colon JJ / Chen S-F / Tsai KL / Murakami K
Funding support United States, 1 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R01-GM123233-01 United States
CitationJournal: Mol Cell / Year: 2023
Title: Structural basis of a transcription pre-initiation complex on a divergent promoter.
Authors: Jose J Gorbea Colón / Leon Palao / Shin-Fu Chen / Hee Jong Kim / Laura Snyder / Yi-Wei Chang / Kuang-Lei Tsai / Kenji Murakami /
Abstract: Most eukaryotic promoter regions are divergently transcribed. As the RNA polymerase II pre-initiation complex (PIC) is intrinsically asymmetric and responsible for transcription in a single ...Most eukaryotic promoter regions are divergently transcribed. As the RNA polymerase II pre-initiation complex (PIC) is intrinsically asymmetric and responsible for transcription in a single direction, it is unknown how divergent transcription arises. Here, the Saccharomyces cerevisiae Mediator complexed with a PIC (Med-PIC) was assembled on a divergent promoter and analyzed by cryoelectron microscopy. The structure reveals two distinct Med-PICs forming a dimer through the Mediator tail module, induced by a homodimeric activator protein localized near the dimerization interface. The tail dimer is associated with ∼80-bp upstream DNA, such that two flanking core promoter regions are positioned and oriented in a suitable form for PIC assembly in opposite directions. Also, cryoelectron tomography visualized the progress of the PIC assembly on the two core promoter regions, providing direct evidence for the role of the Med-PIC dimer in divergent transcription.
History
DepositionMar 29, 2022-
Header (metadata) releaseMar 1, 2023-
Map releaseMar 1, 2023-
UpdateJun 12, 2024-
Current statusJun 12, 2024Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_26551.png

Downloads & links

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Map

FileDownload / File: emd_26551.map.gz / Format: CCP4 / Size: 918.3 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotationcomposite map of Med-PICearly
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.38 Å/pix.
x 603 pix.
= 832.14 Å		1.38 Å/pix.
x 646 pix.
= 891.48 Å		1.38 Å/pix.
x 618 pix.
= 852.84 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

generated in cubic-lattice coordinate

Voxel sizeX=Y=Z: 1.38 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.08
Minimum - Maximum-0.16821769 - 1.857119
Average (Standard dev.)0.00029257126 (±0.013835127)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin-227-127-243
Dimensions646618603
Spacing618646603
CellA: 852.84 Å / B: 891.48 Å / C: 832.14 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : Med-PICearly

EntireName: Med-PICearly
Components
  • Complex: Med-PICearly
    • DNA: DNA (38-MER)
    • DNA: DNA (37-MER)
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 15
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 2
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 3
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 14
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 5
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 16
    • Protein or peptide: Regulatory protein GAL4
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 20
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 7
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 8
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 9
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 10
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 11
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 17
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 18
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 19
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 21
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 22
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 31
    • Protein or peptide: DNA-directed RNA polymerase II subunit RPB1
    • Protein or peptide: DNA-directed RNA polymerase II subunit RPB1
    • Protein or peptide: DNA-directed RNA polymerase II subunit RPB2
    • Protein or peptide: DNA-directed RNA polymerase II subunit RPB3
    • Protein or peptide: DNA-directed RNA polymerase II subunit RPB4
    • Protein or peptide: DNA-directed RNA polymerases I, II, and III subunit RPABC1
    • Protein or peptide: DNA-directed RNA polymerases I, II, and III subunit RPABC2
    • Protein or peptide: DNA-directed RNA polymerase II subunit RPB7
    • Protein or peptide: DNA-directed RNA polymerases I, II, and III subunit RPABC3
    • Protein or peptide: DNA-directed RNA polymerase II subunit RPB9
    • Protein or peptide: DNA-directed RNA polymerases I, II, and III subunit RPABC5
    • Protein or peptide: DNA-directed RNA polymerase II subunit RPB11
    • Protein or peptide: DNA-directed RNA polymerases I, II, and III subunit RPABC4
    • Protein or peptide: Transcription initiation factor IIB
    • Protein or peptide: Transcription initiation factor IIF subunit alpha
    • Protein or peptide: Transcription initiation factor IIF subunit beta
    • Protein or peptide: Transcription elongation factor S-II
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 1
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 4
    • Protein or peptide: Mediator of RNA polymerase II transcription subunit 6
  • Ligand: ZINC ION
  • Ligand: THREONINE
  • Ligand: ALANINE
  • Ligand: ASPARTIC ACID

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Supramolecule #1: Med-PICearly

SupramoleculeName: Med-PICearly / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#41
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)

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Macromolecule #1: DNA (38-MER)

MacromoleculeName: DNA (38-MER) / type: dna / ID: 1 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 11.666479 KDa
SequenceString:
(DA)(DC)(DC)(DG)(DG)(DA)(DG)(DG)(DA)(DC) (DA)(DG)(DT)(DC)(DC)(DT)(DC)(DC)(DC)(DG) (DA)(DC)(DT)(DG)(DA)(DC)(DT)(DG)(DA) (DC)(DG)(DT)(DC)(DG)(DT)(DA)(DC)(DG)

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Macromolecule #2: DNA (37-MER)

MacromoleculeName: DNA (37-MER) / type: dna / ID: 2 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 11.42431 KDa
SequenceString:
(DC)(DG)(DT)(DA)(DC)(DG)(DA)(DC)(DG)(DT) (DC)(DA)(DG)(DT)(DC)(DA)(DG)(DT)(DC)(DG) (DG)(DG)(DA)(DG)(DG)(DA)(DC)(DT)(DG) (DT)(DC)(DC)(DT)(DC)(DC)(DG)(DG)

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Macromolecule #3: Mediator of RNA polymerase II transcription subunit 15

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 15
type: protein_or_peptide / ID: 3 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 120.432242 KDa
SequenceString: MSAAPVQDKD TLSNAERAKN VNGLLQVLMD INTLNGGSSD TADKIRIHAK NFEAALFAKS SSKKEYMDSM NEKVAVMRNT YNTRKNAVT AAAANNNIKP VEQHHINNLK NSGNSANNMN VNMNLNPQMF LNQQAQARQQ VAQQLRNQQQ QQQQQQQQQR R QLTPQQQQ ...String:
MSAAPVQDKD TLSNAERAKN VNGLLQVLMD INTLNGGSSD TADKIRIHAK NFEAALFAKS SSKKEYMDSM NEKVAVMRNT YNTRKNAVT AAAANNNIKP VEQHHINNLK NSGNSANNMN VNMNLNPQMF LNQQAQARQQ VAQQLRNQQQ QQQQQQQQQR R QLTPQQQQ LVNQMKVAPI PKQLLQRIPN IPPNINTWQQ VTALAQQKLL TPQDMEAAKE VYKIHQQLLF KARLQQQQAQ AQ AQANNNN NGLPQNGNIN NNINIPQQQQ MQPPNSSANN NPLQQQSSQN TVPNVLNQIN QIFSPEEQRS LLQEAIETCK NFE KTQLGS TMTEPVKQSF IRKYINQKAL RKIQALRDVK NNNNANNNGS NLQRAQNVPM NIIQQQQQQN TNNNDTIATS ATPN AAAFS QQQNASSKLY QMQQQQQAQA QAQAQAQAQA QAQAQAQAAQ AAQAQAQAQA QAQAQAQAQA QAQAQAQAQA QAQAQ AHAQ HQPSQQPQQA QQQPNPLHGL TPTAKDVEVI KQLSLDASKT NLRLTDVTNS LSNEEKEKIK MKLKQGQKLF VQVSNF APQ VYIITKNENF LKEVFQLRIF VKEILEKCAE GIFVVKLDTV DRLIIKYQKY WESMRIQILR RQAILRQQQQ MANNNGN PG TTSTGNNNNI ATQQNMQQSL QQMQHLQQLK MQQQQQQQQQ QQQQQQQQQQ QQQQHIYPSS TPGVANYSAM ANAPGNNI P YMNHKNTSSM DFLNSMENTP KVPVSAAATP SLNKTINGKV NGRTKSNTIP VTSIPSTNKK LSISNAASQQ PTPRSASNT AKSTPNTNPS PLKTQTKNGT PNPNNMKTVQ SPMGAQPSYN SAIIENAFRK EELLLKDLEI RKLEISSRFK HRQEIFKDSP MDLFMSTLG DCLGIKDEEM LTSCTIPKAV VDHINGSGKR KPTKAAQRAR DQDSIDISIK DNKLVMKSKF NKSNRSYSIA L SNVAAIFK GIGGNFKDLS TLVHSSSPST SSNMDVGNPR KRKASVLEIS PQDSIASVLS PDSNIMSDSK KIKVDSPDDP FM TKSGATT SEKQEVTNEA PFLTSGTSSE QFNVWDWNNW TSAT

UniProtKB: Mediator of RNA polymerase II transcription subunit 15

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Macromolecule #4: Mediator of RNA polymerase II transcription subunit 2

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 2 / type: protein_or_peptide / ID: 4 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 47.75427 KDa
SequenceString: MVVQNSPVSS VHTANFSERG SNTRTMTYKN KLTVCFDDIL KVGAEMMMQQ QLKNVQLDSY LVNGFSQSQQ KLLKEKVKLF HGILDDLET SLSQSSSYLE TLTALGKEKE KEREEAEKKR AEQENMRKVR EQEELKKRQE LEEASQQQQL QQNSKEKNGL G LNFSTTAP ...String:
MVVQNSPVSS VHTANFSERG SNTRTMTYKN KLTVCFDDIL KVGAEMMMQQ QLKNVQLDSY LVNGFSQSQQ KLLKEKVKLF HGILDDLET SLSQSSSYLE TLTALGKEKE KEREEAEKKR AEQENMRKVR EQEELKKRQE LEEASQQQQL QQNSKEKNGL G LNFSTTAP ANTTDANGSK ENYQELGSLQ SSSQTQLENA NAANNGAAFS PLTTTRIQSQ QAQPSDVMFN DLNSMDISMF SG LDSTGFD STAFNATVDE TKGFDDNDSG NNYNDINISS IENNINNNIN STKNGKDNNN ESNKNNNGDE KNKNNNEDNE NNN NSSEKN NNNNNNNNNN NDDNGNNNNN NSGNDNNNTT NNDSNNKNNS ITTGNDNENI VNNDLPTTVV SNPGDNPPPA DNGE EYLTL NDFNDLNIDW STTGDNGELD LSGFNI

UniProtKB: Mediator of RNA polymerase II transcription subunit 2

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Macromolecule #5: Mediator of RNA polymerase II transcription subunit 3

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 3 / type: protein_or_peptide / ID: 5 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 43.116039 KDa
SequenceString: MDSIIPAGVK LDDLQVILAK NENETRDKVC KQINEARDEI LPLRLQFNEF IQIMANIDQE GSKQADRMAK YLHIRDKILQ LNDRFQTLS SHLEALQPLF STVPEYLKTA DNRDRSFQLL EPLSTYNKNG NAVCSTATVV STNHSAAAST PTTTATPHAN P ITHAHSLS ...String:
MDSIIPAGVK LDDLQVILAK NENETRDKVC KQINEARDEI LPLRLQFNEF IQIMANIDQE GSKQADRMAK YLHIRDKILQ LNDRFQTLS SHLEALQPLF STVPEYLKTA DNRDRSFQLL EPLSTYNKNG NAVCSTATVV STNHSAAAST PTTTATPHAN P ITHAHSLS NPNSTATMQH NPLAGKRGPK SGSTMGTPTV HNSTAAAPIA APKKPRKPRQ TKKAKAQAQA QAQAQAQVYA QQ STVQTPI TASMAAALPN PTPSMINSVS PTNVMGTPLT NMMSPMGNAY SMGAQNQGGQ VSMSQFNGSG NGSNPNTNTN SNN TPLQSQ LNLNNLTPAN ILNMSMNNDF QQQQQQQQQQ QQPQPQYNMN MGMNNMNNGG KELDSLDLNN LELGGLNMDF L

UniProtKB: Mediator of RNA polymerase II transcription subunit 3

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Macromolecule #6: Mediator of RNA polymerase II transcription subunit 14

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 14
type: protein_or_peptide / ID: 6 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 123.500695 KDa
SequenceString: MTTTIGSPQM LANEERLSNE MHALKNRSEQ NGQEQQGPVK NTQLHGPSAT DPETTATQKE SLEMVPKDTS AATMTSAPPP ALPHVEINQ VSLALVIRNL TVFTMKELAQ YMKTNVHTQA NEPNSAKKIR FLQLIIFLRT QFLKLYVLVK WTRTIKQNNF H VLIDLLNW ...String:
MTTTIGSPQM LANEERLSNE MHALKNRSEQ NGQEQQGPVK NTQLHGPSAT DPETTATQKE SLEMVPKDTS AATMTSAPPP ALPHVEINQ VSLALVIRNL TVFTMKELAQ YMKTNVHTQA NEPNSAKKIR FLQLIIFLRT QFLKLYVLVK WTRTIKQNNF H VLIDLLNW FRTTNMNVNN CIWALKSSLN SMTNAKLPNV DLVTALEVLS LGRPNLPTHN FKLSGVSNSM DMVDGMAKVP IG LILQRLK DLNLTVSIKI ALMNIPKPLN SYHIKNGRIY FTVPNEFEIQ LSTVNRQSPL FFVDLKLLFN TEAEQTVSAV TEA TSTNGD SENNEENSSS NGNNLPLNKP RLEKLINEIL LKSNDPLLSL YNFLHKYVLT LQLYMVHREF LKLANGGKFS KSNL IHNYD SKKSTITVRY WLNGKMDSKG KITIGIQRTT ESLILKWDNQ SASRAKNMPV IYNNIVSNIE GILDEIMFNH ARIIR SELL ARDIFQEDEE NSDVLLFQLP TTCVSMAPIQ LKIDLLSGQF YFRNPTPLLS NYASKINRAE GPEELARILQ QLKLDK IIH VLTTMFENTG WSCSRIIKID KPIRTQVNTG GESVVKKEDN KYAIAGNSTT NSDVSLLLQR DLFIRLPHWP LNWYLIL SI ISSKTSCVVE KRIGKIVSQR GKWNLKYLDN SNVMTVKLES ITYQKIMILQ RTILNRIINH MLIDSLNQLE IRNKICSS E MINEQKLPQY IIQGSNTNDN ISIITLELES FLEGSKALNS ILESSMFLRI DYSNSQIRLY AKFKRNTMMI QCQIDKLYI HFVQEEPLAF YLEESFTNLG IIVQYLTKFR QKLMQLVVLT DVVERLHKNF ESENFKIIAL QPNEISFKYL SNNDEDDKDC TIKISTNDD SIKNLTVQLS PSNPQHIIQP FLDNSKMDYH FIFSYLQFTS SLFKALKVIL NERGGKFHES GSQYSTMVNI G LHNLNEYQ IVYYNPQAGT KITICIELKT VLHNGRDKIQ FHIHFADVAH ITTKSPAYPM MHQVRNQVFM LDTKRLGTPE SV KPANASH AIRLGNGVAC DPSEIEPILM EIHNILKVDS NSSSS

UniProtKB: Mediator of RNA polymerase II transcription subunit 14

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Macromolecule #7: Mediator of RNA polymerase II transcription subunit 5

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 5 / type: protein_or_peptide / ID: 7 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 128.91875 KDa
SequenceString: MEKESVYNLA LKCAERQLTS MEFSNLYKEF FNEKFPSLIQ EEEEDTTTTA NINEVKKASD LVDTPSNNTA ATADTTHLHE ALDIVCSDF VKILNLEKPL ILADYIVEVL LVNYNSDMIK CFLPKLNSVR NSLLLAHFFS KSCSFFAKLS DTLIIDQVRK D LGNVIVPN ...String:
MEKESVYNLA LKCAERQLTS MEFSNLYKEF FNEKFPSLIQ EEEEDTTTTA NINEVKKASD LVDTPSNNTA ATADTTHLHE ALDIVCSDF VKILNLEKPL ILADYIVEVL LVNYNSDMIK CFLPKLNSVR NSLLLAHFFS KSCSFFAKLS DTLIIDQVRK D LGNVIVPN ILSLDMNSMN KELIAIVSKL LQTTLKLSPS PILLTSAGCK NGSFTLLNQL SQTNKLLFKR VSQTFEAKLH FK DTKPFLN KDSTNEFVGS PSLTSPQYIP SPLSSTKPPG SVNSAAKYKD MKLLRYYKNI WLNNKIINWE ISNPDFLSKY SAI TSSIFQ ESFNSVQNLD QLLTDLIETS FTCFAQFVSN KQYHQANSNL TLLERKWVIF ITKHLPLLIL ENSSRSPRVV TNAL DNIDE KVVKAIRIYF TEKDDNKTNN EDLFDDYPST SLDIRHDFIK GLIMLNLQPA SVINNYLRED QMIDTSILPT RDDLF VRNL QGIQEVVHNT NSFIISSLDT LELESITESI THDSSNGLFQ VLHNFESVAP TKQREIVKAF LSIFEDAIKE LNYNRI AKI CALLFFNFSH SLTTILSFSS PAALMKTLIK FVDLSRNGRN GSNGNDESSE YETINISLSF SWAILLIINL TQTYGIS VV DVALKYPELS IKNSFIINFI SNLPNVSDKY YLEESNVNDS DMLTKSHNTV QSWLCDLFVN GSITDQLIQN IETRQLAN L IPFIVKQVLL SVEIGVLTDI SSLIGGFEYF LQPLLLVGLI KTFYWLEQFL SCVKNDTISE DILQGIFNLL NTLFNPVTL NEDSKAFHTA VLRLNAIPLL KVLRKFRVQS QSNYGIYSSD AQGDPNLEPL IAKLVAVLNV SPVYDVDPRI INSENDYSRK QLGYGKFLI LNENPINKIM TNQINSFWSL HSSTYYNLDY LFELIELVTP KSFLFDVLKT LEYKLATYGV PGSENKRGSL D SEHVFDYF FYFLVLYDVK TAEEASQLIE YMENDAKKSK GDVDIKGEDL HEKNDSAEVR QETQPKAEAT QDDDFDMLFG EN DTSTQAY EEEEENEDND GNNRTNNVPM IKAEETPSKT NKISILKRHS FAVLLHERKL LNDLALENGE ITKTENEKFI SYH DKYLCM LKTCVF

UniProtKB: Mediator of RNA polymerase II transcription subunit 5

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Macromolecule #8: Mediator of RNA polymerase II transcription subunit 16

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 16
type: protein_or_peptide / ID: 8 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 111.423422 KDa
SequenceString: MMLGEHLMSW SKTGIIAYSD SQSSNANICL TFLESINGIN WRFHTPQKYV LHPQLHEVQY QESSSTLSTH STTTSVNGST TAGVGSTPN FGGNSNKSPP QFFYNISSIH WNNWFSLPGD MLAVCDELGN MTMLITGQRP DRATTYEKLT MVFQDNVYKI Y NHVMPLKP ...String:
MMLGEHLMSW SKTGIIAYSD SQSSNANICL TFLESINGIN WRFHTPQKYV LHPQLHEVQY QESSSTLSTH STTTSVNGST TAGVGSTPN FGGNSNKSPP QFFYNISSIH WNNWFSLPGD MLAVCDELGN MTMLITGQRP DRATTYEKLT MVFQDNVYKI Y NHVMPLKP VDKLKPMNIE RKQTRKEYNT SILEFRWLTS SKSVIVSQFC AFDSSSNTYR SRAQQVPPYG VYHPPFIKYA CL AIRKNGQ IDFWYQFSNS KDHKKITLQL LDTSNQRFKD LQWLEFARIT PMNDDQCMLI TTYSKLSKNI SFYKLHVNWN LNA TKPNVL NDPSLKIQFI LSTTLDPTDD EGHVLKLENL HVVSKSSIEK DPSPEILVLY NVCDTSKSLV KRYRLAPTQL SAEY LVILK PDLNIDRNNS TNQIFQSRRY NLRRHSDIVL DKKVTLITSE MFDAFVSFYF EDGTIESYNQ NDWKLETERL ISQSQ LGKF KNIIASPLSA GFNYGKLPLP PSVEWMKVSP SMCGVIVKQY NKKWPQFYAA VQKNYADPEK DSINATALAF GYVKSL HKQ ISAEDLTIAA KTHILRISFL DRKRAKEFIT TLLKSLYSFF NISPDAPKEI MDKIITSRPL QKIMLLQLEL GSCFSQE NI EEMARVILYL KNVLFAFNGV ARNFHFAIEQ ISNNSNQQQN PKLFQTIFSK QDLIHSLIPV AKWFVKFITY LTQEILIL I NDPTNKEYTL VHGIFGAKMS RTLILSILNE IKKVTQIVAK FPETSYPILN ESSTFLKLVL SESPVDFEKF ETFLVDVNN KFIALCEQQP SQEREFSLLV KAEIPPEYAK VGDFLLQYAN NAVISHANAA AVYFADTSGL KISNSEFFNP EIFHLLQPLE EGLIIDTDK LPIKNRTSKS FSKLLYDDVT CDKLSVSEIS DGKLKRCSRC GSVTRAGNII SSDKTIVPTS IQTKRWPTMY T RLCICSGM LFEMDG

UniProtKB: Mediator of RNA polymerase II transcription subunit 16

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Macromolecule #9: Regulatory protein GAL4

MacromoleculeName: Regulatory protein GAL4 / type: protein_or_peptide / ID: 9 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 16.895672 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString:
MKLLSSIEQA CDICRLKKLK CSKEKPKCAK CLKNNWECRY SPKTKRSPLT RAHLTEVESR LERLEQLFLL IFPREDLDMI LKMDSLQDI KALLTGLFVQ DNVNKDAVTD RLASVETDMP LTLRQHRISA TSSSEESSNK GQRQLTVS

UniProtKB: Regulatory protein GAL4

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Macromolecule #10: Mediator of RNA polymerase II transcription subunit 20

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 20
type: protein_or_peptide / ID: 10 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 22.918068 KDa
SequenceString: MGKSAVIFVE RATPATLTEL KDALSNSILS VRDPWSIDFR TYRCSIKNLP ADVSKLMYSI TFHHHGRQTV LIKDNSAMVT TAAAADIPP ALVFNGSSTG VPESIDTILS SKLSNIWMQR QLIKGDAGET LILDGLTVRL VNLFSSTGFK GLLIELQADE A GEFETKIA ...String:
MGKSAVIFVE RATPATLTEL KDALSNSILS VRDPWSIDFR TYRCSIKNLP ADVSKLMYSI TFHHHGRQTV LIKDNSAMVT TAAAADIPP ALVFNGSSTG VPESIDTILS SKLSNIWMQR QLIKGDAGET LILDGLTVRL VNLFSSTGFK GLLIELQADE A GEFETKIA GIEGHLAEIR AKEYKTSSDS LGPDTSNEIC DLAYQYVRAL EL

UniProtKB: Mediator of RNA polymerase II transcription subunit 20

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Macromolecule #11: Mediator of RNA polymerase II transcription subunit 7

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 7 / type: protein_or_peptide / ID: 11 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 25.616062 KDa
SequenceString: MSNDPGNEVS SLYPPPPPYV KFFTQSNLEK LPKYKEKKAA SAKQTAPNNS NGGSEEEITC ALDYLIPPPM PKNQQYRAFG SIWQVKDQL PDLESMGLTQ LYKKSTENES TNYQYKIQEL RKLLKSLLLN YLELIGVLSI NPDMYERKVE NIRTILVNIH H LLNEYRPH ...String:
MSNDPGNEVS SLYPPPPPYV KFFTQSNLEK LPKYKEKKAA SAKQTAPNNS NGGSEEEITC ALDYLIPPPM PKNQQYRAFG SIWQVKDQL PDLESMGLTQ LYKKSTENES TNYQYKIQEL RKLLKSLLLN YLELIGVLSI NPDMYERKVE NIRTILVNIH H LLNEYRPH QSRESLIMLL EEQLEYKRGE IREIEQVCKQ VHDKLTSIQD TLRTGSQSPP SSSQ

UniProtKB: Mediator of RNA polymerase II transcription subunit 7

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Macromolecule #12: Mediator of RNA polymerase II transcription subunit 8

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 8 / type: protein_or_peptide / ID: 12 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 25.297234 KDa
SequenceString: MSQSTASLVP EGNQGSLQED VSFDFNGVPG QALDAVRMRL AQLTHSLRRI RDEMSKAELP QWYTLQSQLN VTLSQLVSVT STLQHFQET LDSTVVYPLP KFPTTSHESL VTTLLRKKNI PEVDEWMKYV RETSGVTTAL LKDEEIEKLL QQDREITNWA R TTFRNEYG ...String:
MSQSTASLVP EGNQGSLQED VSFDFNGVPG QALDAVRMRL AQLTHSLRRI RDEMSKAELP QWYTLQSQLN VTLSQLVSVT STLQHFQET LDSTVVYPLP KFPTTSHESL VTTLLRKKNI PEVDEWMKYV RETSGVTTAL LKDEEIEKLL QQDREITNWA R TTFRNEYG KHDFKNEESL SEEHASLLVR DSKPSKPFNV DDVLKFTFTG EKPIITGSTS TSSSN

UniProtKB: Mediator of RNA polymerase II transcription subunit 8

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Macromolecule #13: Mediator of RNA polymerase II transcription subunit 9

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 9 / type: protein_or_peptide / ID: 13 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 17.400377 KDa
SequenceString:
MNLQNNVLNQ IHQILLPTNP TLDKPNAEAT KEEFSSAENR DEKDYLTNQQ PKNLSTPSTS SNGEFIPHIF YSLHQIRKDP NNLSNQLET LTGSIRHRLK LCKSLISENE DTKDLLSKSP SEWQDIIHQR EQELQIKRDV LDDLYRKLQR

UniProtKB: Mediator of RNA polymerase II transcription subunit 9

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Macromolecule #14: Mediator of RNA polymerase II transcription subunit 10

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 10
type: protein_or_peptide / ID: 14 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 17.927926 KDa
SequenceString:
MNGNSTNNEQ LQQELATTQD QVASIIESFV ELGVSIYDFP GTPEATKGMI TNLQRNVDRL YKLNVRSNDP QSSLSKVDIP LEVVQYIED GRNPDIYTRE FVEAIRRSNQ YQRGKMHGLK QLRDSLADKI VDEFPELKEP VEDIIKRTSP IDNVSNTH

UniProtKB: Mediator of RNA polymerase II transcription subunit 10

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Macromolecule #15: Mediator of RNA polymerase II transcription subunit 11

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 11
type: protein_or_peptide / ID: 15 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 13.324189 KDa
SequenceString:
MQPPYIQERL KSLNDIETQL CSMLQEASQV TFIFGELKRG NESVKPQFEN HVKQFYERLD KSTTQLRKEI QLLDENVGTR LLPINVNKK ALGQDTEKME EQLDLLSAIL DPSKSK

UniProtKB: Mediator of RNA polymerase II transcription subunit 11

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Macromolecule #16: Mediator of RNA polymerase II transcription subunit 17

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 17
type: protein_or_peptide / ID: 16 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 78.582836 KDa
SequenceString: MTTEDPDSNH LSSETGIKLA LDPNLITLAL SSNPNSSLHS PTSDEPVPES AGKADTSIRL EGDELENKTK KDNDKNLKFL KNKDSLVSN PHEIYGSMPL EQLIPIILRQ RGPGFKFVDL NEKELQNEIK QLGSDSSDGH NSEKKDTDGA DENVQIGEDF M EVDYEDKD ...String:
MTTEDPDSNH LSSETGIKLA LDPNLITLAL SSNPNSSLHS PTSDEPVPES AGKADTSIRL EGDELENKTK KDNDKNLKFL KNKDSLVSN PHEIYGSMPL EQLIPIILRQ RGPGFKFVDL NEKELQNEIK QLGSDSSDGH NSEKKDTDGA DENVQIGEDF M EVDYEDKD NPVDSRNETD HKTNENGETD DNIETVMTQE QFVKRRRDML EHINLAMNES SLALEFVSLL LSSVKESTGM SS MSPFLRK VVKPSSLNSD KIPYVAPTKK EYIELDILNK GWKLQSLNES KDLLRASFNK LSSILQNEHD YWNKIMQSIS NKD VIFKIR DRTSGQKLLA IKYGYEDSGS TYKHDRGIAN IRNNIESQNL DLIPHSSSVF KGTDFVHSVK KFLRVRIFTK IESE DDYIL SGESVMDRDS ESEEAETKDI RKQIQLLKKI IFEKELMYQI KKECALLISY GVSIENENKV IIELPNEKFE IELLS LDDD SIVNHEQDLP KINDKRANLM LVMLRLLLVV IFKKTLRSRI SSPHGLINLN VDDDILIIRP ILGKVRFANY KLLLKK IIK DYVLDIVPGS SITETEVERE QPQENKNIDD ENITKLNKEI RAFDKLLNIP RRELKINLPL TEHKSPNLSL MLESPNY CN ALIHIKFSAG TEANAVSFDT TFSDFKEVED FLHFIVAEYI QQKKV

UniProtKB: Mediator of RNA polymerase II transcription subunit 17

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Macromolecule #17: Mediator of RNA polymerase II transcription subunit 18

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 18
type: protein_or_peptide / ID: 17 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 34.316539 KDa
SequenceString: MVQQLSLFGS IGDDGYDLLI STLTTISGNP PLLYNSLCTV WKPNPSYDVE NVNSRNQLVE PNRIKLSKEV PFSYLIDETM MDKPLNFRI LKSFTNDKIP LNYAMTRNIL HNTVPQVTNF NSTNEDQNNS KHTEDTVNES RNSDDIIDVD MDASPAPSNE S CSPWSLQI ...String:
MVQQLSLFGS IGDDGYDLLI STLTTISGNP PLLYNSLCTV WKPNPSYDVE NVNSRNQLVE PNRIKLSKEV PFSYLIDETM MDKPLNFRI LKSFTNDKIP LNYAMTRNIL HNTVPQVTNF NSTNEDQNNS KHTEDTVNES RNSDDIIDVD MDASPAPSNE S CSPWSLQI SDIPAAGNNR SVSMQTIAET IILSSAGKNS SVSSLMNGLG YVFEFQYLTI GVKFFMKHGL ILELQKIWQI EE AGNSQIT SGGFLLKAYI NVSRGTDIDR INYTETALMN LKKELQGYIE LSVPDRQSMD SRVAHGNILI

UniProtKB: Mediator of RNA polymerase II transcription subunit 18

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Macromolecule #18: Mediator of RNA polymerase II transcription subunit 19

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 19
type: protein_or_peptide / ID: 18 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 24.885305 KDa
SequenceString: MASRVDETTV PSYYYYVDPE TTYTYQQPNP LQDLISVYGL DDISRQVART NLDGTKAVKL RKSYKNQIAD LSGKFSTIPT RENGKGGQI AHILFQNNPD MMIQPPQQGQ NMSEQQWREQ LRNRDIALFQ PPNFDWDLCS SVLSQFERSY PSEFANQNQG G AQAPFDID ...String:
MASRVDETTV PSYYYYVDPE TTYTYQQPNP LQDLISVYGL DDISRQVART NLDGTKAVKL RKSYKNQIAD LSGKFSTIPT RENGKGGQI AHILFQNNPD MMIQPPQQGQ NMSEQQWREQ LRNRDIALFQ PPNFDWDLCS SVLSQFERSY PSEFANQNQG G AQAPFDID DLAFDLDGTG KSQSGSNSGN NSKKRKNKSS GSSMATPTHS DSHEDMKRRR LE

UniProtKB: Mediator of RNA polymerase II transcription subunit 19

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Macromolecule #19: Mediator of RNA polymerase II transcription subunit 21

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 21
type: protein_or_peptide / ID: 19 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 16.093368 KDa
SequenceString:
MTDRLTQLQI CLDQMTEQFC ATLNYIDKNH GFERLTVNEP QMSDKHATVV PPEEFSNTID ELSTDIILKT RQINKLIDSL PGVDVSAEE QLRKIDMLQK KLVEVEDEKI EAIKKKEKLL RHVDSLIEDF VDGIANSKKS T

UniProtKB: Mediator of RNA polymerase II transcription subunit 21

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Macromolecule #20: Mediator of RNA polymerase II transcription subunit 22

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 22
type: protein_or_peptide / ID: 20 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 13.774628 KDa
SequenceString:
MSNQALYEKL EQTRTILSVK LAELINMTTI ADRNDDDEGS FAQENSELAV ATTSVMMVNN QTMQLIKNVQ DLLILTRSIK EKWLLNQIP VTEHSKVTRF DEKQIEELLD NCIETFVAEK T

UniProtKB: Mediator of RNA polymerase II transcription subunit 22

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Macromolecule #21: Mediator of RNA polymerase II transcription subunit 31

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 31
type: protein_or_peptide / ID: 21 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 14.747739 KDa
SequenceString:
MSSTNGNAPA TPSSDQNPLP TRFEVELEFI QSLANIQYVT YLLTQQQIWK SPNFKNYLKY LEYWCNPPYS QCIVYPNCLF ILKLLNGFM ESAIVNEDGL LEGLDELPKI IQLQGPQWMN EMVERWAN

UniProtKB: Mediator of RNA polymerase II transcription subunit 31

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Macromolecule #22: DNA-directed RNA polymerase II subunit RPB1

MacromoleculeName: DNA-directed RNA polymerase II subunit RPB1 / type: protein_or_peptide / ID: 22 / Number of copies: 2 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 2.611681 KDa
SequenceString:
PSYSPTSPSY SPTSPSYSPT SPSYS

UniProtKB: DNA-directed RNA polymerase II subunit RPB1

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Macromolecule #23: DNA-directed RNA polymerase II subunit RPB1

MacromoleculeName: DNA-directed RNA polymerase II subunit RPB1 / type: protein_or_peptide / ID: 23 / Number of copies: 2 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 162.951703 KDa
SequenceString: MVGQQYSSAP LRTVKEVQFG LFSPEEVRAI SVAKIRFPET MDETQTRAKI GGLNDPRLGS IDRNLKCQTC QEGMNECPGH FGHIDLAKP VFHVGFIAKI KKVCECVCMH CGKLLLDEHN ELMRQALAIK DSKKRFAAIW TLCKTKMVCE TDVPSEDDPT Q LVSRGGCG ...String:
MVGQQYSSAP LRTVKEVQFG LFSPEEVRAI SVAKIRFPET MDETQTRAKI GGLNDPRLGS IDRNLKCQTC QEGMNECPGH FGHIDLAKP VFHVGFIAKI KKVCECVCMH CGKLLLDEHN ELMRQALAIK DSKKRFAAIW TLCKTKMVCE TDVPSEDDPT Q LVSRGGCG NTQPTIRKDG LKLVGSWKKD RATGDADEPE LRVLSTEEIL NIFKHISVKD FTSLGFNEVF SRPEWMILTC LP VPPPPVR PSISFNESQR GEDDLTFKLA DILKANISLE TLEHNGAPHH AIEEAESLLQ FHVATYMDND IAGQPQALQK SGR PVKSIR ARLKGKEGRI RGNLMGKRVD FSARTVISGD PNLELDQVGV PKSIAKTLTY PEVVTPYNID RLTQLVRNGP NEHP GAKYV IRDSGDRIDL RYSKRAGDIQ LQYGWKVERH IMDNDPVLFN RQPSLHKMSM MAHRVKVIPY STFRLNLSVT SPYNA DFDG DEMNLHVPQS EETRAELSQL CAVPLQIVSP QSNKPCMGIV QDTLCGIRKL TLRDTFIELD QVLNMLYWVP DWDGVI PTP AIIKPKPLWS GKQILSVAIP NGIHLQRFDE GTTLLSPKDN GMLIIDGQII FGVVEKKTVG SSNGGLIHVV TREKGPQ VC AKLFGNIQKV VNFWLLHNGF STGIGDTIAD GPTMREITET IAEAKKKVLD VTKEAQANLL TAKHGMTLRE SFEDNVVR F LNEARDKAGR LAEVNLKDLN NVKQMVMAGS KGSFINIAQM SACVGQQSVE GKRIAFGFVD RTLPHFSKDD YSPESKGFV ENSYLRGLTP QEFFFHAMGG REGLIDTAVK TAETGYIQRR LVKALEDIMV HYDNTTRNSL GNVIQFIYGE DGMDAAHIEK QSLDTIGGS DAAFEKRYRV DLLNTDHTLD PSLLESGSEI LGDLKLQVLL DEEYKQLVKD RKFLREVFVD GEANWPLPVN I RRIIQNAQ QTFHIDHTKP SDLTIKDIVL GVKDLQENLL VLRGKNEIIQ NAQRDAVTLF CCLLRSRLAT RRVLQEYRLT KQ AFDWVLS NIEAQFLRSV VHPGEMVGVL AAQSIGEPAT QMTLNTFHFA GVASKKVTSG VPRLKEILNV AKNMKTPSLT VYL EPGHAA DQEQAKLIRS AIEHTTLKSV TIASEIYYDP DPRSTVIPED EEIIQLHFSL LDEEAEQSFD QQSPWLLRLE LDRA AMNDK DLTMGQVGER IKQTFKNDLF VIWSEDNDEK LIIRCRVVRP KSLDAETEAE EDHMLKKIEN TMLENITLRG VENIE RVVM MKYDRKVPSP TGEYVKEPEW VLETDGVNLS EVMTVPGIDP TRIYTNSFID IMEVLGIEAG RAALYKEVYN VIASDG SYV NYRHMALLVD VMTTQGGLTS VTRHGFNRSN TGALMRCSFE ETVEILFEAG ASAELDDCRG VSENVILGQM APIGTGA FD VMIDEESLVK Y

UniProtKB: DNA-directed RNA polymerase II subunit RPB1

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Macromolecule #24: DNA-directed RNA polymerase II subunit RPB2

MacromoleculeName: DNA-directed RNA polymerase II subunit RPB2 / type: protein_or_peptide / ID: 24 / Number of copies: 2 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 138.937297 KDa
SequenceString: MSDLANSEKY YDEDPYGFED ESAPITAEDS WAVISAFFRE KGLVSQQLDS FNQFVDYTLQ DIICEDSTLI LEQLAQHTTE SDNISRKYE ISFGKIYVTK PMVNESDGVT HALYPQEARL RNLTYSSGLF VDVKKRTYEA IDVPGRELKY ELIAEESEDD S ESGKVFIG ...String:
MSDLANSEKY YDEDPYGFED ESAPITAEDS WAVISAFFRE KGLVSQQLDS FNQFVDYTLQ DIICEDSTLI LEQLAQHTTE SDNISRKYE ISFGKIYVTK PMVNESDGVT HALYPQEARL RNLTYSSGLF VDVKKRTYEA IDVPGRELKY ELIAEESEDD S ESGKVFIG RLPIMLRSKN CYLSEATESD LYKLKECPFD MGGYFIINGS EKVLIAQERS AGNIVQVFKK AAPSPISHVA EI RSALEKG SRFISTLQVK LYGREGSSAR TIKATLPYIK QDIPIVIIFR ALGIIPDGEI LEHICYDVND WQMLEMLKPC VED GFVIQD RETALDFIGR RGTALGIKKE KRIQYAKDIL QKEFLPHITQ LEGFESRKAF FLGYMINRLL LCALDRKDQD DRDH FGKKR LDLAGPLLAQ LFKTLFKKLT KDIFRYMQRT VEEAHDFNMK LAINAKTITS GLKYALATGN WGEQKKAMSS RAGVS QVLN RYTYSSTLSH LRRTNTPIGR DGKLAKPRQL HNTHWGLVCP AETPEGQACG LVKNLSLMSC ISVGTDPMPI ITFLSE WGM EPLEDYVPHQ SPDATRVFVN GVWHGVHRNP ARLMETLRTL RRKGDINPEV SMIRDIREKE LKIFTDAGRV YRPLFIV ED DESLGHKELK VRKGHIAKLM ATEYQDIEGG FEDVEEYTWS SLLNEGLVEY IDAEEEESIL IAMQPEDLEP AEANEEND L DVDPAKRIRV SHHATTFTHC EIHPSMILGV AASIIPFPDH NQSPRNTYQS AMGKQAMGVF LTNYNVRMDT MANILYYPQ KPLGTTRAME YLKFRELPAG QNAIVAIACY SGYNQEDSMI MNQSSIDRGL FRSLFFRSYM DQEKKYGMSI TETFEKPQRT NTLRMKHGT YDKLDDDGLI APGVRVSGED VIIGKTTPIS PDEEELGQRT AYHSKRDAST PLRSTENGIV DQVLVTTNQD G LKFVKVRV RTTKIPQIGD KFASRHGQKG TIGITYRRED MPFTAEGIVP DLIINPHAIP SRMTVAHLIE CLLSKVAALS GN EGDASPF TDITVEGISK LLREHGYQSR GFEVMYNGHT GKKLMAQIFF GPTYYQRLRH MVDDKIHARA RGPMQVLTRQ PVE GRSRDG GLRFGEMERD CMIAHGAASF LKERLMEASD AFRVHICGIC GLMTVIAKLN HNQFECKGCD NKIDIYQIHI PYAA KLLFQ ELMAMNITPR LYTDRSRDF

UniProtKB: DNA-directed RNA polymerase II subunit RPB2

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Macromolecule #25: DNA-directed RNA polymerase II subunit RPB3

MacromoleculeName: DNA-directed RNA polymerase II subunit RPB3 / type: protein_or_peptide / ID: 25 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 35.330457 KDa
SequenceString: MSEEGPQVKI REASKDNVDF ILSNVDLAMA NSLRRVMIAE IPTLAIDSVE VETNTTVLAD EFIAHRLGLI PLQSMDIEQL EYSRDCFCE DHCDKCSVVL TLQAFGESES TTNVYSKDLV IVSNLMGRNI GHPIIQDKEG NGVLICKLRK GQELKLTCVA K KGIAKEHA ...String:
MSEEGPQVKI REASKDNVDF ILSNVDLAMA NSLRRVMIAE IPTLAIDSVE VETNTTVLAD EFIAHRLGLI PLQSMDIEQL EYSRDCFCE DHCDKCSVVL TLQAFGESES TTNVYSKDLV IVSNLMGRNI GHPIIQDKEG NGVLICKLRK GQELKLTCVA K KGIAKEHA KWGPAAAIEF EYDPWNKLKH TDYWYEQDSA KEWPQSKNCE YEDPPNEGDP FDYKAQADTF YMNVESVGSI PV DQVVVRG IDTLQKKVAS ILLALTQMDQ DKVNFASGDN NTASNMLGSN EDVMMTGAEQ DPYSNASQMG NTGSGGYDNA W

UniProtKB: DNA-directed RNA polymerase II subunit RPB3

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Macromolecule #26: DNA-directed RNA polymerase II subunit RPB4

MacromoleculeName: DNA-directed RNA polymerase II subunit RPB4 / type: protein_or_peptide / ID: 26 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 25.451191 KDa
SequenceString: MNVSTSTFQT RRRRLKKVEE EENAATLQLG QEFQLKQINH QGEEEELIAL NLSEARLVIK EALVERRRAF KRSQKKHKKK HLKHENAND ETTAVEDEDD DLDEDDVNAD DDDFMHSETR EKELESIDVL LEQTTGGNNK DLKNTMQYLT NFSRFRDQET V GAVIQLLK ...String:
MNVSTSTFQT RRRRLKKVEE EENAATLQLG QEFQLKQINH QGEEEELIAL NLSEARLVIK EALVERRRAF KRSQKKHKKK HLKHENAND ETTAVEDEDD DLDEDDVNAD DDDFMHSETR EKELESIDVL LEQTTGGNNK DLKNTMQYLT NFSRFRDQET V GAVIQLLK STGLHPFEVA QLGSLACDTA DEAKTLIPSL NNKISDDELE RILKELSNLE TLY

UniProtKB: DNA-directed RNA polymerase II subunit RPB4

+
Macromolecule #27: DNA-directed RNA polymerases I, II, and III subunit RPABC1

MacromoleculeName: DNA-directed RNA polymerases I, II, and III subunit RPABC1
type: protein_or_peptide / ID: 27 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 25.117094 KDa
SequenceString: MDQENERNIS RLWRAFRTVK EMVKDRGYFI TQEEVELPLE DFKAKYCDSM GRPQRKMMSF QANPTEESIS KFPDMGSLWV EFCDEPSVG VKTMKTFVIH IQEKNFQTGI FVYQNNITPS AMKLVPSIPP ATIETFNEAA LVVNITHHEL VPKHIRLSSD E KRELLKRY ...String:
MDQENERNIS RLWRAFRTVK EMVKDRGYFI TQEEVELPLE DFKAKYCDSM GRPQRKMMSF QANPTEESIS KFPDMGSLWV EFCDEPSVG VKTMKTFVIH IQEKNFQTGI FVYQNNITPS AMKLVPSIPP ATIETFNEAA LVVNITHHEL VPKHIRLSSD E KRELLKRY RLKESQLPRI QRADPVALYL GLKRGEVVKI IRKSETSGRY ASYRICM

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC1

+
Macromolecule #28: DNA-directed RNA polymerases I, II, and III subunit RPABC2

MacromoleculeName: DNA-directed RNA polymerases I, II, and III subunit RPABC2
type: protein_or_peptide / ID: 28 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 17.931834 KDa
SequenceString:
MSDYEEAFND GNENFEDFDV EHFSDEETYE EKPQFKDGET TDANGKTIVT GGNGPEDFQQ HEQIRRKTLK EKAIPKDQRA TTPYMTKYE RARILGTRAL QISMNAPVFV DLEGETDPLR IAMKELAEKK IPLVIRRYLP DGSFEDWSVE ELIVDL

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC2

+
Macromolecule #29: DNA-directed RNA polymerase II subunit RPB7

MacromoleculeName: DNA-directed RNA polymerase II subunit RPB7 / type: protein_or_peptide / ID: 29 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 19.081053 KDa
SequenceString:
MFFIKDLSLN ITLHPSFFGP RMKQYLKTKL LEEVEGSCTG KFGYILCVLD YDNIDIQRGR ILPTDGSAEF NVKYRAVVFK PFKGEVVDG TVVSCSQHGF EVQVGPMKVF VTKHLMPQDL TFNAGSNPPS YQSSEDVITI KSRIRVKIEG CISQVSSIHA I GSIKEDYL GAI

UniProtKB: DNA-directed RNA polymerase II subunit RPB7

+
Macromolecule #30: DNA-directed RNA polymerases I, II, and III subunit RPABC3

MacromoleculeName: DNA-directed RNA polymerases I, II, and III subunit RPABC3
type: protein_or_peptide / ID: 30 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 16.525363 KDa
SequenceString:
MSNTLFDDIF QVSEVDPGRY NKVCRIEAAS TTQDQCKLTL DINVELFPVA AQDSLTVTIA SSLNLEDTPA NDSSATRSWR PPQAGDRSL ADDYDYVMYG TAYKFEEVSK DLIAVYYSFG GLLMRLEGNY RNLNNLKQEN AYLLIRR

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC3

+
Macromolecule #31: DNA-directed RNA polymerase II subunit RPB9

MacromoleculeName: DNA-directed RNA polymerase II subunit RPB9 / type: protein_or_peptide / ID: 31 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 14.308161 KDa
SequenceString:
MTTFRFCRDC NNMLYPREDK ENNRLLFECR TCSYVEEAGS PLVYRHELIT NIGETAGVVQ DIGSDPTLPR SDRECPKCHS RENVFFQSQ QRRKDTSMVL FFVCLSCSHI FTSDQKNKRT QFS

UniProtKB: DNA-directed RNA polymerase II subunit RPB9

+
Macromolecule #32: DNA-directed RNA polymerases I, II, and III subunit RPABC5

MacromoleculeName: DNA-directed RNA polymerases I, II, and III subunit RPABC5
type: protein_or_peptide / ID: 32 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 8.290732 KDa
SequenceString:
MIVPVRCFSC GKVVGDKWES YLNLLQEDEL DEGTALSRLG LKRYCCRRMI LTHVDLIEKF LRYNPLEKRD

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC5

+
Macromolecule #33: DNA-directed RNA polymerase II subunit RPB11

MacromoleculeName: DNA-directed RNA polymerase II subunit RPB11 / type: protein_or_peptide / ID: 33 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 13.633493 KDa
SequenceString:
MNAPDRFELF LLGEGESKLK IDPDTKAPNA VVITFEKEDH TLGNLIRAEL LNDRKVLFAA YKVEHPFFAR FKLRIQTTEG YDPKDALKN ACNSIINKLG ALKTNFETEW NLQTLAADDA F

UniProtKB: DNA-directed RNA polymerase II subunit RPB11

+
Macromolecule #34: DNA-directed RNA polymerases I, II, and III subunit RPABC4

MacromoleculeName: DNA-directed RNA polymerases I, II, and III subunit RPABC4
type: protein_or_peptide / ID: 34 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 7.729969 KDa
SequenceString:
MSREGFQIPT NLDAAAAGTS QARTATLKYI CAECSSKLSL SRTDAVRCKD CGHRILLKAR TKRLVQFEAR

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC4

+
Macromolecule #35: Transcription initiation factor IIB

MacromoleculeName: Transcription initiation factor IIB / type: protein_or_peptide / ID: 35 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 38.25734 KDa
SequenceString: MMTRESIDKR AGRRGPNLNI VLTCPECKVY PPKIVERFSE GDVVCALCGL VLSDKLVDTR SEWRTFSNDD HNGDDPSRVG EASNPLLDG NNLSTRIGKG ETTDMRFTKE LNKAQGKNVM DKKDNEVQAA FAKITMLCDA AELPKIVKDC AKEAYKLCHD E KTLKGKSM ...String:
MMTRESIDKR AGRRGPNLNI VLTCPECKVY PPKIVERFSE GDVVCALCGL VLSDKLVDTR SEWRTFSNDD HNGDDPSRVG EASNPLLDG NNLSTRIGKG ETTDMRFTKE LNKAQGKNVM DKKDNEVQAA FAKITMLCDA AELPKIVKDC AKEAYKLCHD E KTLKGKSM ESIMAASILI GCRRAEVART FKEIQSLIHV KTKEFGKTLN IMKNILRGKS EDGFLKIDTD NMSGAQNLTY IP RFCSHLG LPMQVTTSAE YTAKKCKEIK EIAGKSPITI AVVSIYLNIL LFQIPITAAK VGQTLQVTEG TIKSGYKILY EHR DKLVDP QLIANGVVSL DNLPGVEKK

UniProtKB: Transcription initiation factor IIB

+
Macromolecule #36: Transcription initiation factor IIF subunit alpha

MacromoleculeName: Transcription initiation factor IIF subunit alpha / type: protein_or_peptide / ID: 36 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 82.32057 KDa
SequenceString: MSRRNPPGSR NGGGPTNASP FIKRDRMRRN FLRMRMGQNG SNSSSPGVPN GDNSRGSLVK KDDPEYAEER EKMLLQIGVE ADAGRSNVK VKDEDPNEYN EFPLRAIPKE DLENMRTHLL KFQSKKKINP VTDFHLPVRL HRKDTRNLQF QLTRAEIVQR Q KEISEYKK ...String:
MSRRNPPGSR NGGGPTNASP FIKRDRMRRN FLRMRMGQNG SNSSSPGVPN GDNSRGSLVK KDDPEYAEER EKMLLQIGVE ADAGRSNVK VKDEDPNEYN EFPLRAIPKE DLENMRTHLL KFQSKKKINP VTDFHLPVRL HRKDTRNLQF QLTRAEIVQR Q KEISEYKK KAEQERSTPN SGGMNKSGTV SLNNTVKDGS QTPTVDSVTK DNTANGVNSS IPTVTGSSVP PASPTTVSAV ES NGLSNGS TSAANGLDGN ASTANLANGR PLVTKLEDAG PAEDPTKVGM VKYDGKEVTN EPEFEEGTMD PLADVAPDGG GRA KRGNLR RKTRQLKVLD ENAKKLRFEE FYPWVMEDFD GYNTWVGSYE AGNSDSYVLL SVEDDGSFTM IPADKVYKFT ARNK YATLT IDEAEKRMDK KSGEVPRWLM KHLDNIGTTT TRYDRTRRKL KAVADQQAMD EDDRDDNSEV ELDYDEEFAD DEEAP IIDG NEQENKESEQ RIKKEMLQAN AMGLRDEEAP SENEEDELFG EKKIDEDGER IKKALQKTEL AALYSSDENE INPYLS ESD IENKENESPV KKEEDSDTLS KSKRSSPKKQ QKKATNAHVH KEPTLRVKSI KNCVIILKGD KKILKSFPEG EWNPQTT KA VDSSNNASNT VPSPIKQEEG LNSTVAEREE TPAPTITEKD IIEAIGDGKV NIKEFGKFIR RKYPGAENKK LMFAIVKK L CRKVGNDHME LKKE

UniProtKB: Transcription initiation factor IIF subunit alpha

+
Macromolecule #37: Transcription initiation factor IIF subunit beta

MacromoleculeName: Transcription initiation factor IIF subunit beta / type: protein_or_peptide / ID: 37 / Number of copies: 2 / Enantiomer: LEVO / EC number: DNA helicase
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 46.684492 KDa
SequenceString: MSSGSAGAPA LSNNSTNSVA KEKSGNISGD EYLSQEEEVF DGNDIENNET KVYEESLDLD LERSNRQVWL VRLPMFLAEK WRDRNNLHG QELGKIRINK DGSKITLLLN ENDNDSIPHE YDLELTKKVV ENEYVFTEQN LKKYQQRKKE LEADPEKQRQ A YLKKQERE ...String:
MSSGSAGAPA LSNNSTNSVA KEKSGNISGD EYLSQEEEVF DGNDIENNET KVYEESLDLD LERSNRQVWL VRLPMFLAEK WRDRNNLHG QELGKIRINK DGSKITLLLN ENDNDSIPHE YDLELTKKVV ENEYVFTEQN LKKYQQRKKE LEADPEKQRQ A YLKKQERE EELKKKQQQQ KRRNNRKKFN HRVMTDRDGR DRYIPYVKTI PKKTAIVGTV CHECQVMPSM NDPNYHKIVE QR RNIVKLN NKERITTLDE TVGVTMSHTG MSMRSDNSNF LKVGREKAKS NIKSIRMPKK EILDYLFKLF DEYDYWSLKG LKE RTRQPE AHLKECLDKV ATLVKKGPYA FKYTLRPEYK KLKEEERKAT LGELADEQTG SAGDNAQGDA EADLEDEIEM EDVV

UniProtKB: Transcription initiation factor IIF subunit beta

+
Macromolecule #38: Transcription elongation factor S-II

MacromoleculeName: Transcription elongation factor S-II / type: protein_or_peptide / ID: 38 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 34.903551 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MDSKEVLVHV KNLEKNKSND AAVLEILHVL DKEFVPTEKL LRETKVGVEV NKFKKSTNVE ISKLVKKMIS SWKDAINKNK RSRQAQQHH QDHAPGNAED KTTVGESVNG VQQPASSQSD AMKQDKYVST KPRNSKNDGV DTAIYHHKLR DQVLKALYDV L AKESEHPP ...String:
MDSKEVLVHV KNLEKNKSND AAVLEILHVL DKEFVPTEKL LRETKVGVEV NKFKKSTNVE ISKLVKKMIS SWKDAINKNK RSRQAQQHH QDHAPGNAED KTTVGESVNG VQQPASSQSD AMKQDKYVST KPRNSKNDGV DTAIYHHKLR DQVLKALYDV L AKESEHPP QSILHTAKAI ESEMNKVNNC DTNEAAYKAR YRIIYSNVIS KNNPDLKHKI ANGDITPEFL ATCDAKDLAP AP LKQKIEE IAKQNLYNAQ GATIERSVTD RFTCGKCKEK KVSYYQLQTR SADEPLTTFC TCEACGNRWK FS

UniProtKB: Transcription elongation factor S-II

+
Macromolecule #39: Mediator of RNA polymerase II transcription subunit 1

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 1 / type: protein_or_peptide / ID: 39 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 64.314953 KDa
SequenceString: MVEGDSYVET LDSMIELFKD YKPGSITLEN ITRLCQTLGL ESFTEELSNE LSRLSTASKI IVIDVDYNKK QDRIQDVKLV LASNFDNFD YFNQRDGEHE KSNILLNSLT KYPDLKAFHN NLKFLYLLDA YSHIESDSTS HNNGSSDKSL DSSNASFNNQ G KLDLFKYF ...String:
MVEGDSYVET LDSMIELFKD YKPGSITLEN ITRLCQTLGL ESFTEELSNE LSRLSTASKI IVIDVDYNKK QDRIQDVKLV LASNFDNFD YFNQRDGEHE KSNILLNSLT KYPDLKAFHN NLKFLYLLDA YSHIESDSTS HNNGSSDKSL DSSNASFNNQ G KLDLFKYF TELSHYIRQC FQDNCCDFKV RTNLNDKFGI YILTQGINGK EVPLAKIYLE ENKSDSQYRF YEYIYSQETK SW INESAEN FSNGISLVME IVANAKESNY TDLIWFPEDF ISPELIIDKV TCSSNSSSSP PIIDLFSNNN YNSRIQLMND FTT KLINIK KFDISNDNLD LISEILKWVQ WSRIVLQNVF KLVSTPSSNS NSSELEPDYQ APFSTSTKDK NSSTSNTEPI PRSN RHGSV VEASRRRRSS TNKSKRPSIT EAMMLKEEGL QQFNLHEILS EPAIEEENGD SIKEHSTTMD GANDLGFTAS VSNQE NAGT DIVMEDHGVL QGTSQNYGTA TADDADIEMK DVSSKPSKPE SSVLQLIVSE DHIILDTISE CNLYDDVKCW SKFIEK FQD IVS

UniProtKB: Mediator of RNA polymerase II transcription subunit 1

+
Macromolecule #40: Mediator of RNA polymerase II transcription subunit 4

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 4 / type: protein_or_peptide / ID: 40 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 32.244895 KDa
SequenceString: MSVQDTKAVE FSMGHIRSSS VSLVAEATSN TNSEDKLSKV QLYEDLCRYE DTLSKLVESV DRFKPNLDIA KDLIRTDEAL FENVKLLAE YDNIYRNLQK IDKDSEELDS KTRKILEILN ECHDELKALP MLEQVEFEKN TILQQRSKIN STELLDYATK L SKFTKIPP ...String:
MSVQDTKAVE FSMGHIRSSS VSLVAEATSN TNSEDKLSKV QLYEDLCRYE DTLSKLVESV DRFKPNLDIA KDLIRTDEAL FENVKLLAE YDNIYRNLQK IDKDSEELDS KTRKILEILN ECHDELKALP MLEQVEFEKN TILQQRSKIN STELLDYATK L SKFTKIPP TFDKGAVGPN NFIWPAEDAL RRGMLAMASL HSKELTRIPG EEVEETEVPT VPPSQSEEQK GQMAKKEGTP KT DSFIFDG TAKEVGDEAD NTKDKEKEEN NDDALDLDLD LFDPDDF

UniProtKB: Mediator of RNA polymerase II transcription subunit 4

+
Macromolecule #41: Mediator of RNA polymerase II transcription subunit 6

MacromoleculeName: Mediator of RNA polymerase II transcription subunit 6 / type: protein_or_peptide / ID: 41 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 32.844746 KDa
SequenceString: MNVTPLDELQ WKSPEWIQVF GLRTENVLDY FAESPFFDKT SNNQVIKMQR QFSQLNDPNA AVNMTQNIMT LPDGKNGNLE EEFAYVDPA RRQILFKYPM YMQLEEELMK LDGTEYVLSS VREPDFWVIR KQRRTNNSGV GSAKGPEIIP LQDYYIIGAN I YQSPTIFK ...String:
MNVTPLDELQ WKSPEWIQVF GLRTENVLDY FAESPFFDKT SNNQVIKMQR QFSQLNDPNA AVNMTQNIMT LPDGKNGNLE EEFAYVDPA RRQILFKYPM YMQLEEELMK LDGTEYVLSS VREPDFWVIR KQRRTNNSGV GSAKGPEIIP LQDYYIIGAN I YQSPTIFK IVQSRLMSTS YHLNSTLESL YDLIEFQPSQ GVHYKVPTDT STTATAATNG NNAGGGSNKS SVRPTGGANM AT VPSTTNV NMTVNTMGTG GQTIDNGTGR TGNGNMGITT EMLDKLMVTS IRSTPNYI

UniProtKB: Mediator of RNA polymerase II transcription subunit 6

+
Macromolecule #42: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 42 / Number of copies: 4 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

+
Macromolecule #43: THREONINE

MacromoleculeName: THREONINE / type: ligand / ID: 43 / Number of copies: 1 / Formula: THR
Molecular weightTheoretical: 119.119 Da
Chemical component information

ChemComp-THR:
THREONINE

+
Macromolecule #44: ALANINE

MacromoleculeName: ALANINE / type: ligand / ID: 44 / Number of copies: 1 / Formula: ALA
Molecular weightTheoretical: 89.093 Da
Chemical component information

ChemComp-ALA:
ALANINE

+
Macromolecule #45: ASPARTIC ACID

MacromoleculeName: ASPARTIC ACID / type: ligand / ID: 45 / Number of copies: 1 / Formula: ASP
Molecular weightTheoretical: 133.103 Da
Chemical component information

ChemComp-ASP:
ASPARTIC ACID

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

Concentration0.1 mg/mL
BufferpH: 7.6
GridModel: Quantifoil / Pretreatment - Type: PLASMA CLEANING / Pretreatment - Time: 60 sec.
VitrificationCryogen name: ETHANE / Instrument: LEICA EM CPC

-
Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average exposure time: 3.4 sec. / Average electron dose: 42.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsCalibrated defocus max: 3.0 µm / Calibrated defocus min: 0.5 µm / Calibrated magnification: 64000 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 3.0 µm / Nominal defocus min: 0.5 µm / Nominal magnification: 64000
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: INSILICO MODEL
Final reconstructionNumber classes used: 1 / Algorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 3.3 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 1102984
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: PROJECTION MATCHING

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Atomic model buiding 1

Initial model
PDB IDChain

7ui9

source_name: PDB, initial_model_type: experimental model

7uil

source_name: PDB, initial_model_type: experimental model

7uik

source_name: PDB, initial_model_type: experimental model
RefinementSpace: REAL / Protocol: RIGID BODY FIT / Target criteria: Cross-correlation
Output model

PDB-7uio:
Mediator-PIC Early (Composite Model)

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