登録情報 データベース : EMDB / ID : EMD-22363 構造の表示 ダウンロードとリンクタイトル Structure of Drosophila ORC in the active conformation マップデータunsharpened, unmasked cryo-EM map 詳細 試料複合体 : Drosophila ORC (active state)タンパク質・ペプチド : Origin recognition complex subunit 4タンパク質・ペプチド : Origin recognition complex subunit 5タンパク質・ペプチド : Origin recognition complex subunit 1タンパク質・ペプチド : Origin recognition complex subunit 2タンパク質・ペプチド : Origin recognition complex subunit 3タンパク質・ペプチド : Origin recognition complex subunit 6 残り2件を表示 表示を減らすリガンド : ADENOSINE-5'-TRIPHOSPHATEリガンド : MAGNESIUM ION 詳細 キーワード origin recognition complex / DNA replication initiation / REPLICATION機能・相同性 機能・相同性情報分子機能 ドメイン・相同性 構成要素
alpha-heterochromatin / larval feeding behavior / CDC6 association with the ORC:origin complex / septin cytoskeleton / septin cytoskeleton organization / Activation of ATR in response to replication stress / Activation of the pre-replicative complex / eggshell chorion gene amplification / Orc1 removal from chromatin / origin recognition complex ... alpha-heterochromatin / larval feeding behavior / CDC6 association with the ORC:origin complex / septin cytoskeleton / septin cytoskeleton organization / Activation of ATR in response to replication stress / Activation of the pre-replicative complex / eggshell chorion gene amplification / Orc1 removal from chromatin / origin recognition complex / DNA amplification / positive regulation of border follicle cell migration / Assembly of the ORC complex at the origin of replication / nuclear origin of replication recognition complex / olfactory learning / nuclear pre-replicative complex / DNA replication preinitiation complex / mitotic DNA replication checkpoint signaling / mitotic chromosome condensation / chromosome condensation / DNA replication origin binding / DNA replication initiation / heterochromatin / ribonucleoprotein complex binding / nuclear pore / GTPase activator activity / mitotic spindle organization / learning / DNA-templated DNA replication / mitotic cell cycle / DNA replication / learning or memory / chromatin binding / protein homodimerization activity / ATP hydrolysis activity / DNA binding / ATP binding / nucleus / metal ion binding / plasma membrane 類似検索 - 分子機能 Origin recognition complex, subunit 6, metazoa/plant / Origin recognition complex subunit 3, insertion domain / Origin recognition complex subunit 3, N-terminal / Origin recognition complex (ORC) subunit 3 N-terminus / Origin recognition complex subunit 3 insertion domain / Origin recognition complex, subunit 6 / Origin recognition complex subunit 6 (ORC6) / Cdc6, C-terminal / CDC6, C terminal / CDC6, C terminal winged helix domain ... Origin recognition complex, subunit 6, metazoa/plant / Origin recognition complex subunit 3, insertion domain / Origin recognition complex subunit 3, N-terminal / Origin recognition complex (ORC) subunit 3 N-terminus / Origin recognition complex subunit 3 insertion domain / Origin recognition complex, subunit 6 / Origin recognition complex subunit 6 (ORC6) / Cdc6, C-terminal / CDC6, C terminal / CDC6, C terminal winged helix domain / Origin recognition complex subunit 4 / Origin recognition complex, subunit 3 / Origin recognition complex, subunit 5 / Origin recognition complex subunit 4, C-terminal / Origin recognition complex subunit 3, winged helix C-terminal / : / : / Origin recognition complex (ORC) subunit 4 C-terminus / Origin recognition complex (ORC) subunit 5 C-terminus / Origin recognition complex winged helix C-terminal / ORC5, lid domain / Orc1-like, AAA ATPase domain / AAA ATPase domain / Origin recognition complex subunit 2 / Origin recognition complex, subunit 2 / AAA lid domain / AAA lid domain / Bromo adjacent homology (BAH) domain superfamily / Bromo adjacent homology domain / Bromo adjacent homology (BAH) domain / BAH domain / BAH domain profile. / ATPase family associated with various cellular activities (AAA) / ATPase, AAA-type, core / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / P-loop containing nucleoside triphosphate hydrolase 類似検索 - ドメイン・相同性 Origin recognition complex subunit 1 / Origin recognition complex subunit 2 / Origin recognition complex subunit 5 / Origin recognition complex subunit 3 / Origin recognition complex subunit 4 / Origin recognition complex subunit 6 類似検索 - 構成要素生物種 Drosophila melanogaster (キイロショウジョウバエ)手法 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度 : 4.0 Å 詳細 データ登録者Schmidt JM / Bleichert F 資金援助 スイス, 1件 詳細 詳細を隠すOrganization Grant number 国 European Research Council (ERC) ERC-STG-757909 スイス
引用ジャーナル : Nat Commun / 年 : 2020タイトル : Structural mechanism for replication origin binding and remodeling by a metazoan origin recognition complex and its co-loader Cdc6.著者 : Jan Marten Schmidt / Franziska Bleichert / 要旨 : Eukaryotic DNA replication initiation relies on the origin recognition complex (ORC), a DNA-binding ATPase that loads the Mcm2-7 replicative helicase onto replication origins. Here, we report cryo- ... Eukaryotic DNA replication initiation relies on the origin recognition complex (ORC), a DNA-binding ATPase that loads the Mcm2-7 replicative helicase onto replication origins. Here, we report cryo-electron microscopy (cryo-EM) structures of DNA-bound Drosophila ORC with and without the co-loader Cdc6. These structures reveal that Orc1 and Orc4 constitute the primary DNA binding site in the ORC ring and cooperate with the winged-helix domains to stabilize DNA bending. A loop region near the catalytic Walker B motif of Orc1 directly contacts DNA, allosterically coupling DNA binding to ORC's ATPase site. Correlating structural and biochemical data show that DNA sequence modulates DNA binding and remodeling by ORC, and that DNA bending promotes Mcm2-7 loading in vitro. Together, these findings explain the distinct DNA sequence-dependencies of metazoan and S. cerevisiae initiators in origin recognition and support a model in which DNA geometry and bendability contribute to Mcm2-7 loading site selection in metazoans. 履歴 登録 2020年7月27日 - ヘッダ(付随情報) 公開 2020年9月9日 - マップ公開 2020年9月9日 - 更新 2024年3月6日 - 現状 2024年3月6日 処理サイト : RCSB / 状態 : 公開
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