+データを開く
-基本情報
登録情報 | データベース: EMDB / ID: EMD-21658 | |||||||||
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タイトル | Cryo-EM structure of human Cohesin-NIPBL-DNA complex | |||||||||
マップデータ | Human Cohesin-NIPBL-DNA complex | |||||||||
試料 |
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キーワード | Protein-DNA complex / ATPase / DNA-binding protein / Genome organization / Sister chromatid cohesion / Transcription regulation / CELL CYCLE / CELL CYCLE-DNA complex | |||||||||
機能・相同性 | 機能・相同性情報 eye morphogenesis / cohesin loader activity / external genitalia morphogenesis / gallbladder development / SMC loading complex / Scc2-Scc4 cohesin loading complex / ear morphogenesis / mitotic cohesin loading / regulation of hair cycle / response to DNA damage checkpoint signaling ...eye morphogenesis / cohesin loader activity / external genitalia morphogenesis / gallbladder development / SMC loading complex / Scc2-Scc4 cohesin loading complex / ear morphogenesis / mitotic cohesin loading / regulation of hair cycle / response to DNA damage checkpoint signaling / forelimb morphogenesis / maintenance of mitotic sister chromatid cohesion / negative regulation of mitotic metaphase/anaphase transition / embryonic viscerocranium morphogenesis / positive regulation of sister chromatid cohesion / Cohesin Loading onto Chromatin / meiotic cohesin complex / Establishment of Sister Chromatid Cohesion / establishment of meiotic sister chromatid cohesion / cohesin complex / mitotic cohesin complex / uterus morphogenesis / embryonic digestive tract morphogenesis / negative regulation of G2/M transition of mitotic cell cycle / establishment of protein localization to chromatin / regulation of developmental growth / positive regulation of neuron migration / negative regulation of glial cell apoptotic process / cellular response to X-ray / integrator complex / digestive tract development / chromo shadow domain binding / lateral element / replication-born double-strand break repair via sister chromatid exchange / developmental growth / mediator complex binding / establishment of mitotic sister chromatid cohesion / positive regulation of multicellular organism growth / metanephros development / positive regulation of ossification / chromatin looping / embryonic forelimb morphogenesis / reciprocal meiotic recombination / face morphogenesis / lncRNA binding / sister chromatid cohesion / negative regulation of interleukin-1 beta production / microtubule motor activity / stem cell population maintenance / mitotic sister chromatid cohesion / dynein complex binding / fat cell differentiation / mitotic spindle pole / beta-tubulin binding / outflow tract morphogenesis / regulation of DNA replication / somatic stem cell population maintenance / mitotic sister chromatid segregation / regulation of embryonic development / positive regulation of interleukin-10 production / negative regulation of tumor necrosis factor production / chromosome, centromeric region / mitotic spindle assembly / heart morphogenesis / SUMOylation of DNA damage response and repair proteins / protein localization to chromatin / Resolution of Sister Chromatid Cohesion / Meiotic synapsis / condensed nuclear chromosome / meiotic cell cycle / cognition / chromosome segregation / promoter-specific chromatin binding / sensory perception of sound / brain development / response to radiation / kinetochore / histone deacetylase binding / spindle pole / nuclear matrix / Separation of Sister Chromatids / transcription corepressor activity / double-strand break repair / protein localization / mitotic cell cycle / chromosome / double-stranded DNA binding / midbody / DNA-binding transcription factor binding / DNA recombination / Estrogen-dependent gene expression / negative regulation of neuron apoptotic process / nuclear body / response to hypoxia / chromatin remodeling / protein heterodimerization activity / cell division / intracellular membrane-bounded organelle / DNA repair / DNA damage response 類似検索 - 分子機能 | |||||||||
生物種 | Homo sapiens (ヒト) | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 5.3 Å | |||||||||
データ登録者 | Shi ZB / Gao H / Yu H / Bai X | |||||||||
資金援助 | 米国, 2件
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引用 | ジャーナル: Science / 年: 2020 タイトル: Cryo-EM structure of the human cohesin-NIPBL-DNA complex. 著者: Zhubing Shi / Haishan Gao / Xiao-Chen Bai / Hongtao Yu / 要旨: As a ring-shaped adenosine triphosphatase (ATPase) machine, cohesin organizes the eukaryotic genome by extruding DNA loops and mediates sister chromatid cohesion by topologically entrapping DNA. How ...As a ring-shaped adenosine triphosphatase (ATPase) machine, cohesin organizes the eukaryotic genome by extruding DNA loops and mediates sister chromatid cohesion by topologically entrapping DNA. How cohesin executes these fundamental DNA transactions is not understood. Using cryo-electron microscopy (cryo-EM), we determined the structure of human cohesin bound to its loader NIPBL and DNA at medium resolution. Cohesin and NIPBL interact extensively and together form a central tunnel to entrap a 72-base pair DNA. NIPBL and DNA promote the engagement of cohesin's ATPase head domains and ATP binding. The hinge domains of cohesin adopt an "open washer" conformation and dock onto the STAG1 subunit. Our structure explains the synergistic activation of cohesin by NIPBL and DNA and provides insight into DNA entrapment by cohesin. | |||||||||
履歴 |
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-構造の表示
ムービー |
ムービービューア |
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構造ビューア | EMマップ: SurfViewMolmilJmol/JSmol |
添付画像 |
-ダウンロードとリンク
-EMDBアーカイブ
マップデータ | emd_21658.map.gz | 49.1 MB | EMDBマップデータ形式 | |
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ヘッダ (付随情報) | emd-21658-v30.xml emd-21658.xml | 22.7 KB 22.7 KB | 表示 表示 | EMDBヘッダ |
FSC (解像度算出) | emd_21658_fsc.xml | 8.6 KB | 表示 | FSCデータファイル |
画像 | emd_21658.png | 120.5 KB | ||
Filedesc metadata | emd-21658.cif.gz | 9.8 KB | ||
アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-21658 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-21658 | HTTPS FTP |
-検証レポート
文書・要旨 | emd_21658_validation.pdf.gz | 610.9 KB | 表示 | EMDB検証レポート |
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文書・詳細版 | emd_21658_full_validation.pdf.gz | 610.4 KB | 表示 | |
XML形式データ | emd_21658_validation.xml.gz | 10.5 KB | 表示 | |
CIF形式データ | emd_21658_validation.cif.gz | 13.7 KB | 表示 | |
アーカイブディレクトリ | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-21658 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-21658 | HTTPS FTP |
-関連構造データ
-リンク
EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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「今月の分子」の関連する項目 |
-マップ
ファイル | ダウンロード / ファイル: emd_21658.map.gz / 形式: CCP4 / 大きさ: 52.7 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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注釈 | Human Cohesin-NIPBL-DNA complex | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.34 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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-添付データ
-試料の構成要素
-全体 : Human Cohesin-NIPBL-DNA Complex
全体 | 名称: Human Cohesin-NIPBL-DNA Complex |
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要素 |
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-超分子 #1: Human Cohesin-NIPBL-DNA Complex
超分子 | 名称: Human Cohesin-NIPBL-DNA Complex / タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: #1-#7 |
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由来(天然) | 生物種: Homo sapiens (ヒト) |
分子量 | 理論値: 820 KDa |
-分子 #1: Structural maintenance of chromosomes protein 1A
分子 | 名称: Structural maintenance of chromosomes protein 1A / タイプ: protein_or_peptide / ID: 1 / コピー数: 1 / 光学異性体: LEVO |
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由来(天然) | 生物種: Homo sapiens (ヒト) |
分子量 | 理論値: 143.484109 KDa |
組換発現 | 生物種: Trichoplusia ni (イラクサキンウワバ) |
配列 | 文字列: MGFLKLIEIE NFKSYKGRQI IGPFQRFTAI IGPNGSGKSN LMDAISFVLG EKTSNLRVKT LRDLIHGAPV GKPAANRAFV SMVYSEEGA EDRTFARVIV GGSSEYKINN KVVQLHEYSE ELEKLGILIK ARNFLVFQGA VESIAMKNPK ERTALFEEIS R SGELAQEY ...文字列: MGFLKLIEIE NFKSYKGRQI IGPFQRFTAI IGPNGSGKSN LMDAISFVLG EKTSNLRVKT LRDLIHGAPV GKPAANRAFV SMVYSEEGA EDRTFARVIV GGSSEYKINN KVVQLHEYSE ELEKLGILIK ARNFLVFQGA VESIAMKNPK ERTALFEEIS R SGELAQEY DKRKKEMVKA EEDTQFNYHR KKNIAAERKE AKQEKEEADR YQRLKDEVVR AQVQLQLFKL YHNEVEIEKL NK ELASKNK EIEKDKKRMD KVEDELKEKK KELGKMMREQ QQIEKEIKEK DSELNQKRPQ YIKAKENTSH KIKKLEAAKK SLQ NAQKHY KKRKGDMDEL EKEMLSVEKA RQEFEERMEE ESQSQGRDLT LEENQVKKYH RLKEEASKRA ATLAQELEKF NRDQ KADQD RLDLEERKKV ETEAKIKQKL REIEENQKRI EKLEEYITTS KQSLEEQKKL EGELTEEVEM AKRRIDEINK ELNQV MEQL GDARIDRQES SRQQRKAEIM ESIKRLYPGS VYGRLIDLCQ PTQKKYQIAV TKVLGKNMDA IIVDSEKTGR DCIQYI KEQ RGEPETFLPL DYLEVKPTDE KLRELKGAKL VIDVIRYEPP HIKKALQYAC GNALVCDNVE DARRIAFGGH QRHKTVA LD GTLFQKSGVI SGGASDLKAK ARRWDEKAVD KLKEKKERLT EELKEQMKAK RKEAELRQVQ SQAHGLQMRL KYSQSDLE Q TKTRHLALNL QEKSKLESEL ANFGPRINDI KRIIQSRERE MKDLKEKMNQ VEDEVFEEFC REIGVRNIRE FEEEKVKRQ NEIAKKRLEF ENQKTRLGIQ LDFEKNQLKE DQDKVHMWEQ TVKKDENEIE KLKKEEQRHM KIIDETMAQL QDLKNQHLAK KSEVNDKNH EMEEIRKKLG GANKEMTHLQ KEVTAIETKL EQKRSDRHNL LQACKMQDIK LPLSKGTMDD ISQEEGSSQG E DSVSGSQR ISSIYAREAL IEIDYGDLCE DLKDAQAEEE IKQEMNTLQQ KLNEQQSVLQ RIAAPNMKAM EKLESVRDKF QE TSDEFEA ARKRAKKAKQ AFEQIKKERF DRFNACFESV ATNIDEIYKA LSRNSSAQAF LGPENPEEPY LDGINYNCVA PGK RFRPMD NLSGGEKTVA ALALLFAIHS YKPAPFFVLD QIDAALDNTN IGKVANYIKE QSTCNFQAIV ISLKEEFYTK AESL IGVYP EQGDCVISKV LTFDLTKYPD ANPNPNEQ UniProtKB: Structural maintenance of chromosomes protein 1A |
-分子 #2: Structural maintenance of chromosomes protein 3
分子 | 名称: Structural maintenance of chromosomes protein 3 / タイプ: protein_or_peptide / ID: 2 / コピー数: 1 / 光学異性体: LEVO |
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由来(天然) | 生物種: Homo sapiens (ヒト) |
分子量 | 理論値: 141.770578 KDa |
組換発現 | 生物種: Trichoplusia ni (イラクサキンウワバ) |
配列 | 文字列: MYIKQVIIQG FRSYRDQTIV DPFSSKHNVI VGRNGSGKSN FFYAIQFVLS DEFSHLRPEQ RLALLHEGTG PRVISAFVEI IFDNSDNRL PIDKEEVSLR RVIGAKKDQY FLDKKMVTKN DVMNLLESAG FSRSNPYYIV KQGKINQMAT APDSQRLKLL R EVAGTRVY ...文字列: MYIKQVIIQG FRSYRDQTIV DPFSSKHNVI VGRNGSGKSN FFYAIQFVLS DEFSHLRPEQ RLALLHEGTG PRVISAFVEI IFDNSDNRL PIDKEEVSLR RVIGAKKDQY FLDKKMVTKN DVMNLLESAG FSRSNPYYIV KQGKINQMAT APDSQRLKLL R EVAGTRVY DERKEESISL MKETEGKREK INELLKYIEE RLHTLEEEKE ELAQYQKWDK MRRALEYTIY NQELNETRAK LD ELSAKRE TSGEKSRQLR DAQQDARDKM EDIERQVREL KTKISAMKEE KEQLSAERQE QIKQRTKLEL KAKDLQDELA GNS EQRKRL LKERQKLLEK IEEKQKELAE TEPKFNSVKE KEERGIARLA QATQERTDLY AKQGRGSQFT SKEERDKWIK KELK SLDQA INDKKRQIAA IHKDLEDTEA NKEKNLEQYN KLDQDLNEVK ARVEELDRKY YEVKNKKDEL QSERNYLWRE ENAEQ QALA AKREDLEKKQ QLLRAATGKA ILNGIDSINK VLDHFRRKGI NQHVQNGYHG IVMNNFECEP AFYTCVEVTA GNRLFY HIV DSDEVSTKIL MEFNKMNLPG EVTFLPLNKL DVRDTAYPET NDAIPMISKL RYNPRFDKAF KHVFGKTLIC RSMEVST QL ARAFTMDCIT LEGDQVSHRG ALTGGYYDTR KSRLELQKDV RKAEEELGEL EAKLNENLRR NIERINNEID QLMNQMQQ I ETQQRKFKAS RDSILSEMKM LKEKRQQSEK TFMPKQRSLQ SLEASLHAME STRESLKAEL GTDLLSQLSL EDQKRVDAL NDEIRQLQQE NRQLLNERIK LEGIITRVET YLNENLRKRL DQVEQELNEL RETEGGTVLT ATTSELEAIN KRVKDTMARS EDLDNSIDK TEAGIKELQK SMERWKNMEK EHMDAINHDT KELEKMTNRQ GMLLKKKEEC MKKIRELGSL PQEAFEKYQT L SLKQLFRK LEQCNTELKK YSHVNKKALD QFVNFSEQKE KLIKRQEELD RGYKSIMELM NVLELRKYEA IQLTFKQVSK NF SEVFQKL VPGGKATLVM KKGDVEGSQS QDEGEGSGES ERGSGSQSSV PSVDQFTGVG IRVSFTGKQG EMREMQQLSG GQK SLVALA LIFAIQKCDP APFYLFDQID QALDAQHRKA VSDMIMELAV HAQFITTTFR PELLESADKF YGVKFRNKVS HIDV ITAEM AKDFVEDDTT HG UniProtKB: Structural maintenance of chromosomes protein 3 |
-分子 #3: Double-strand-break repair protein rad21 homolog
分子 | 名称: Double-strand-break repair protein rad21 homolog / タイプ: protein_or_peptide / ID: 3 / コピー数: 1 / 光学異性体: LEVO |
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由来(天然) | 生物種: Homo sapiens (ヒト) |
分子量 | 理論値: 71.556102 KDa |
組換発現 | 生物種: Trichoplusia ni (イラクサキンウワバ) |
配列 | 文字列: MFYAHFVLSK RGPLAKIWLA AHWDKKLTKA HVFECNLESS VESIISPKVK MALRTSGHLL LGVVRIYHRK AKYLLADCNE AFIKIKMAF RPGVVDLPEE NREAAYNAIT LPEEFHDFDQ PLPDLDDIDV AQQFSLNQSR VEEITMREEV GNISILQEND F GDFGMDDR ...文字列: MFYAHFVLSK RGPLAKIWLA AHWDKKLTKA HVFECNLESS VESIISPKVK MALRTSGHLL LGVVRIYHRK AKYLLADCNE AFIKIKMAF RPGVVDLPEE NREAAYNAIT LPEEFHDFDQ PLPDLDDIDV AQQFSLNQSR VEEITMREEV GNISILQEND F GDFGMDDR EIMAEGSAFE DDDMLVSTTT SNLLLESEQS TSNLNEKINH LEYEDQYKDD NFGEGNDGGI LDDKLISNND GG IFDDPPA LSEAGVMLPE QPAHDDMDED DNVSMGGPDS PASVDPVEPM PTMTDQTTLV PNEEEAFALE PIDITVKETK AKR KRKLIV DSVKELDSKT IRAQLSDYSD IVTTLDLAPP TKKLMMWKET GGVEKLFSLP AQPLWNNRLL KLFTRCLTPL VPED LRKRR KGGEADNLDE FLKEFENPEV PREDQQQQHQ QRDVIDEPII EEPSALQESV MEASRTNIDE SAMPPPPPQG VKRKA GQID PEPVMPPQQV EQMEIPPVEL PPEEPPNICQ LIPELELLPE KEKEKEKEKE DDEEEEDEDA SGGDQDQEER RWNKRT QQM LHGLQRALAK TGAESISLLE LCRNTNRKQA AAKFYSFLVL KKQQAIELTQ EEPYSDIIAT PGPRFHII UniProtKB: Double-strand-break repair protein rad21 homolog |
-分子 #4: Cohesin subunit SA-1
分子 | 名称: Cohesin subunit SA-1 / タイプ: protein_or_peptide / ID: 4 / コピー数: 1 / 光学異性体: LEVO |
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由来(天然) | 生物種: Homo sapiens (ヒト) |
分子量 | 理論値: 146.075656 KDa |
組換発現 | 生物種: Trichoplusia ni (イラクサキンウワバ) |
配列 | 文字列: MITSELPVLQ DSTNETTAHS DAGSELEETE VKGKRKRGRP GRPPSTNKKP RKSPGEKSRI EAGIRGAGRG RANGHPQQNG EGEPVTLFE VVKLGKSAMQ SVVDDWIESY KQDRDIALLD LINFFIQCSG CRGTVRIEMF RNMQNAEIIR KMTEEFDEDS G DYPLTMPG ...文字列: MITSELPVLQ DSTNETTAHS DAGSELEETE VKGKRKRGRP GRPPSTNKKP RKSPGEKSRI EAGIRGAGRG RANGHPQQNG EGEPVTLFE VVKLGKSAMQ SVVDDWIESY KQDRDIALLD LINFFIQCSG CRGTVRIEMF RNMQNAEIIR KMTEEFDEDS G DYPLTMPG PQWKKFRSNF CEFIGVLIRQ CQYSIIYDEY MMDTVISLLT GLSDSQVRAF RHTSTLAAMK LMTALVNVAL NL SIHQDNT QRQYEAERNK MIGKRANERL ELLLQKRKEL QENQDEIENM MNSIFKGIFV HRYRDAIAEI RAICIEEIGV WMK MYSDAF LNDSYLKYVG WTLHDRQGEV RLKCLKALQS LYTNRELFPK LELFTNRFKD RIVSMTLDKE YDVAVEAIRL VTLI LHGSE EALSNEDCEN VYHLVYSAHR PVAVAAGEFL HKKLFSRHDP QAEEALAKRR GRNSPNGNLI RMLVLFFLES ELHEH AAYL VDSLWESSQE LLKDWECMTE LLLEEPVQGE EAMSDRQESA LIELMVCTIR QAAEAHPPVG RGTGKRVLTA KERKTQ IDD RNKLTEHFII TLPMLLSKYS ADAEKVANLL QIPQYFDLEI YSTGRMEKHL DALLKQIKFV VEKHVESDVL EACSKTY SI LCSEEYTIQN RVDIARSQLI DEFVDRFNHS VEDLLQEGEE ADDDDIYNVL STLKRLTSFH NAHDLTKWDL FGNCYRLL K TGIEHGAMPE QIVVQALQCS HYSILWQLVK ITDGSPSKED LLVLRKTVKS FLAVCQQCLS NVNTPVKEQA FMLLCDLLM IFSHQLMTGG REGLQPLVFN PDTGLQSELL SFVMDHVFID QDEENQSMEG DEEDEANKIE ALHKRRNLLA AFSKLIIYDI VDMHAAADI FKHYMKYYND YGDIIKETLS KTRQIDKIQC AKTLILSLQQ LFNELVQEQG PNLDRTSAHV SGIKELARRF A LTFGLDQI KTREAVATLH KDGIEFAFKY QNQKGQEYPP PNLAFLEVLS EFSSKLLRQD KKTVHSYLEK FLTEQMMERR ED VWLPLIS YRNSLVTGGE DDRMSVNSGS SSSKTSSVRN KKGRPPLHKK RVEDESLDNT WLNRTDTMIQ TPGPLPAPQL TST VLRENS RPMGDQIQEP ESEHGSEPDF LHNPQMQISW LGQPKLEDLN RKDRTGMNYM KVRTGVRHAV RGLMEEDAEP IFED VMMSS RSQLEDMNEE FEDTMVIDLP PSRNRRERAE LRPDFFDSAA IIEDDSGFGM PMFGAPMRSG ALEVLFQ UniProtKB: Cohesin subunit SA-1 |
-分子 #5: Nipped-B-like protein
分子 | 名称: Nipped-B-like protein / タイプ: protein_or_peptide / ID: 5 / コピー数: 1 / 光学異性体: LEVO |
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由来(天然) | 生物種: Homo sapiens (ヒト) |
分子量 | 理論値: 188.151688 KDa |
組換発現 | 生物種: Trichoplusia ni (イラクサキンウワバ) |
配列 | 文字列: MSYYHHHHHH PSLSEVARKM KKKEKQKKRK AYEPKLTPEE MMDSSTFKRF TASIENILDN LEDMDFTAFG DDDEIPQELL LGKHQLNEL GSESAKIKAM GIMDKLSTDK TVKVLNILEK NIQDGSKLST LLNHNNDTEE EERLWRDLIM ERVTKSADAC L TTINIMTS ...文字列: MSYYHHHHHH PSLSEVARKM KKKEKQKKRK AYEPKLTPEE MMDSSTFKRF TASIENILDN LEDMDFTAFG DDDEIPQELL LGKHQLNEL GSESAKIKAM GIMDKLSTDK TVKVLNILEK NIQDGSKLST LLNHNNDTEE EERLWRDLIM ERVTKSADAC L TTINIMTS PNMPKAVYIE DVIERVIQYT KFHLQNTLYP QYDPVYRLDP HGGGLLSSKA KRAKCSTHKQ RVIVMLYNKV CD IVSSLSE LLEIQLLTDT TILQVSSMGI TPFFVENVSE LQLCAIKLVT AVFSRYEKHR QLILEEIFTS LARLPTSKRS LRN FRLNSS DMDGEPMYIQ MVTALVLQLI QCVVHLPSSE KDSNAEEDSN KKIDQDVVIT NSYETAMRTA QNFLSIFLKK CGSK QGEED YRPLFENFVQ DLLSTVNKPE WPAAELLLSL LGRLLVHQFS NKSTEMALRV ASLDYLGTVA ARLRKDAVTS KMDQG SIER ILKQVSGGED EIQQLQKALL DYLDENTETD PSLVFSRKFY IAQWFRDTTL ETEKAMKSQK DEESSEGTHH AKEIET TGQ IMHRAENRKK FLRSIIKTTP SQFSTLKMNS DTVDYDDACL IVRYLASMRP FAQSFDIYLT QILRVLGENA IAVRTKA MK CLSEVVAVDP SILARLDMQR GVHGRLMDNS TSVREAAVEL LGRFVLCRPQ LAEQYYDMLI ERILDTGISV RKRVIKIL R DICIEQPTFP KITEMCVKMI RRVNDEEGIK KLVNETFQKL WFTPTPHNDK EAMTRKILNI TDVVAACRDT GYDWFEQLL QNLLKSEEDS SYKPVKKACT QLVDNLVEHI LKYEESLADS DNKGVNSGRL VACITTLFLF SKIRPQLMVK HAMTMQPYLT TKCSTQNDF MVICNVAKIL ELVVPLMEHP SETFLATIEE DLMKLIIKYG MTVVQHCVSC LGAVVNKVTQ NFKFVWACFN R YYGAISKL KSQHQEDPNN TSLLTNKPAL LRSLFTVGAL CRHFDFDLED FKGNSKVNIK DKVLELLMYF TKHSDEEVQT KA IIGLGFA FIQHPSLMFE QEVKNLYNNI LSDKNSSVNL KIQVLKNLQT YLQEEDTRMQ QADRDWKKVA KQEDLKEMGD VSS GMSSSI MQLYLKQVLE AFFHTQSSVR HFALNVIALT LNQGLIHPVQ CVPYLIAMGT DPEPAMRNKA DQQLVEIDKK YAGF IHMKA VAGMKMSYQV QQAINTCLKD PVRGFRQDES SSALCSHLYS MIRGNRQHRR AFLISLLNLF DDTAKTDVTM LLYIA DNLA CFPYQTQEEP LFIMHHIDIT LSVSGSNLLQ SFKESMVKDK RKERKSSPSK ENESSDSEEE VSRPRKSRKR VDSDSD SDS EDDINSVMKC LPENSAPLIE FANVSQGILL LLMLKQHLKN LCGFSDSKIQ KYSPSESAKV YDKAINRKTG VHFHPKQ TL DFLRSDMANS KITEEVKRSI VKQYLDFKLL MEHLDPDEEE EEGEVSASTN ARNKAITSLL GGGSPKNNTA AETEDDES D GEDRGGGTSG SLRRSKRNSD STELAAQMNE SVDVMDVIAI CCPKYKDRPQ IARVVQKTSS GFSVQWMAGS YSGSWTEAK RRDGRKLVPW VDTIKESDII YKKIALTSAN KLTNKVVQTL RSLYAAKDGT SS UniProtKB: Nipped-B-like protein |
-分子 #6: DNA (51-MER)
分子 | 名称: DNA (51-MER) / タイプ: dna / ID: 6 / コピー数: 1 / 分類: DNA |
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由来(天然) | 生物種: Homo sapiens (ヒト) |
分子量 | 理論値: 15.928584 KDa |
配列 | 文字列: (DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA) (DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA) (DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA) (DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA) (DA) (DA)(DA)(DA)(DA)(DA) ...文字列: (DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA) (DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA) (DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA) (DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA) (DA) (DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA) (DA)(DA)(DA) |
-分子 #7: DNA (51-MER)
分子 | 名称: DNA (51-MER) / タイプ: dna / ID: 7 / コピー数: 1 / 分類: DNA |
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由来(天然) | 生物種: Homo sapiens (ヒト) |
分子量 | 理論値: 15.468875 KDa |
配列 | 文字列: (DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT) (DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT) (DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT) (DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT) (DT) (DT)(DT)(DT)(DT)(DT) ...文字列: (DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT) (DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT) (DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT) (DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT) (DT) (DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT) (DT)(DT)(DT) |
-分子 #8: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER
分子 | 名称: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / タイプ: ligand / ID: 8 / コピー数: 2 / 式: ANP |
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分子量 | 理論値: 506.196 Da |
Chemical component information | ChemComp-ANP: |
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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解析 | 単粒子再構成法 |
試料の集合状態 | particle |
-試料調製
緩衝液 | pH: 7.5 |
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凍結 | 凍結剤: ETHANE / チャンバー内湿度: 100 % / チャンバー内温度: 277.15 K / 装置: FEI VITROBOT MARK IV |
-電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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特殊光学系 | エネルギーフィルター - 名称: GIF Quantum LS / エネルギーフィルター - スリット幅: 20 eV |
撮影 | フィルム・検出器のモデル: GATAN K3 (6k x 4k) / 実像数: 5796 / 平均電子線量: 60.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD |
試料ステージ | 試料ホルダーモデル: FEI TITAN KRIOS AUTOGRID HOLDER |
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |
+画像解析
-原子モデル構築 1
精密化 | 空間: REAL |
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得られたモデル | PDB-6wg3: |