National Institutes of Health/National Cancer Institute (NIH/NCI)
HSSN261200800001E
United States
National Institutes of Health/National Cancer Institute (NIH/NCI)
R01CA212403
United States
National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI)
R21GM131231
United States
National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI)
GM111367
United States
National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI)
GM093271
United States
National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI)
U24GM116788
United States
Citation
Journal: To Be Published Title: A 3.4-angstrom cryo-EM structure of the apo form of hu-man PRMT5:MEP50 complex reveals sequential catalysis of symmetric methyl transfer Authors: Zhou W / Yadav GP / Jiang Q-X / Li C
History
Deposition
Sep 26, 2019
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Header (metadata) release
Jan 29, 2020
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Map release
Dec 9, 2020
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Update
Mar 20, 2024
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Current status
Mar 20, 2024
Processing site: RCSB / Status: Released
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Structure visualization
Movie
Surface view with section colored by density value
Protein or peptide: Protein arginine N-methyltransferase 5
Protein or peptide: Methylosome protein 50
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Supramolecule #1: apo PRMT5:MEP50 complex
Supramolecule
Name: apo PRMT5:MEP50 complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all Details: the apo form of PRMT5:MEP50 heterooctomeric complex
Source (natural)
Organism: Homo sapiens (human)
Molecular weight
Theoretical: 445 KDa
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Macromolecule #1: Protein arginine N-methyltransferase 5
Macromolecule
Name: Protein arginine N-methyltransferase 5 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO / EC number: type II protein arginine methyltransferase
Model: Quantifoil R1.2/1.3 / Material: GOLD / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY / Support film - Film thickness: 12 / Pretreatment - Type: PLASMA CLEANING / Pretreatment - Time: 20 sec. / Pretreatment - Atmosphere: OTHER
Vitrification
Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK II
Details
This sample was monodisperse.
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Electron microscopy
Microscope
FEI TITAN KRIOS
Temperature
Min: 93.0 K / Max: 123.0 K
Image recording
Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Digitization - Frames/image: 2-39 / Average electron dose: 40.0 e/Å2
Electron beam
Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron optics
C2 aperture diameter: 100.0 µm / Illumination mode: OTHER / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: -4.0 µm / Nominal defocus min: -1.0 µm
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