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- EMDB-19515: Structure of IFTA and IFTB in Retrograde Intraflagellar transport... -

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基本情報

登録情報
データベース: EMDB / ID: EMD-19515
タイトルStructure of IFTA and IFTB in Retrograde Intraflagellar transport trains
マップデータ
試料
  • 細胞: In situ retrograde intraflagellar transport trains in dhc1b-3 mutant Chlamydomonas reinhardtii cilia
    • タンパク質・ペプチド: x 20種
キーワードCilia / IFT / train / retrograde / PROTEIN TRANSPORT
機能・相同性
機能・相同性情報


intraciliary transport particle / intraciliary transport particle B binding / intraciliary transport involved in cilium assembly / intraciliary transport particle B / system development / intraciliary transport particle A / outer dynein arm assembly / cilium-dependent cell motility / intraciliary retrograde transport / intraciliary transport ...intraciliary transport particle / intraciliary transport particle B binding / intraciliary transport involved in cilium assembly / intraciliary transport particle B / system development / intraciliary transport particle A / outer dynein arm assembly / cilium-dependent cell motility / intraciliary retrograde transport / intraciliary transport / animal organ development / regulation of cilium assembly / axoneme assembly / axonemal microtubule / cilium organization / protein localization to cilium / ciliary plasm / non-motile cilium assembly / non-motile cilium / motile cilium / post-transcriptional regulation of gene expression / ciliary membrane / ciliary base / microtubule organizing center / cilium assembly / axoneme / kinesin binding / RNA processing / beta-tubulin binding / regulation of microtubule cytoskeleton organization / centriole / regulation of protein stability / tubulin binding / apical part of cell / intracellular protein localization / protein transport / microtubule binding / protein stabilization / cilium / ciliary basal body / GTPase activity / GTP binding / metal ion binding / cytoplasm
類似検索 - 分子機能
Clusterin-associated protein-1 / Intra-flagellar transport protein 57 / Intraflagellar transport protein 20 / Clusterin-associated protein-1 / Intra-flagellar transport protein 57 / Intraflagellar transport complex B, subunit 20 / Intraflagellar transport complex B protein 46 / Intraflagellar transport protein 74 / Tetratricopeptide repeat protein 30 / : ...Clusterin-associated protein-1 / Intra-flagellar transport protein 57 / Intraflagellar transport protein 20 / Clusterin-associated protein-1 / Intra-flagellar transport protein 57 / Intraflagellar transport complex B, subunit 20 / Intraflagellar transport complex B protein 46 / Intraflagellar transport protein 74 / Tetratricopeptide repeat protein 30 / : / : / : / Intraflagellar transport complex B protein 46 C terminal / IFT80 second beta-propeller / IFT52 central domain / IFT52 GIFT domain / Intraflagellar transport protein 25 / Intraflagellar transport protein 81 / IFT81, calponin homology domain / IFT81, N-terminal domain superfamily / Intraflagellar transport 81 calponin homology domain / Intraflagellar transport protein 52 homolog / TRAF3-interacting protein 1, N-terminal / TRAF3-interacting protein 1, C-terminal domain / TRAF3-interacting protein 1, N-terminal domain superfamily / : / Microtubule-binding protein MIP-T3 CH-like domain / Microtubule-binding protein MIP-T3 C-terminal region / Intraflagellar transport protein 52, C-terminal domain / TRAF3-interacting protein 1 / : / IFT122, zinc ribbon domain / Tetratricopeptide repeat protein 21A/21B / : / : / : / : / Tetratricopeptide repeat protein 21 ARM repeat / Tetratricopeptide repeat protein 21 second ARM domain / Tetratricopeptide repeat protein 21 N-terminal ARM repeat / Tetratricopeptide repeat protein 21 C-terminal ARM domain / TT21 fifth ARM repeats domain / Tetratricopeptide repeat / WD repeat protein 35 / WDR19, WD40 repeat / WD repeat-containing protein 19/dyf-2 / : / : / : / : / : / : / : / : / WDR19 second beta-propeller / IFT140 first beta-propeller / IFT140 second beta-propeller / WDR19 first beta-propeller / WDR35 second beta-propeller / IF140 C-terminal TPR domain / WDR35/TULP4 N-terminal / WD repeat-containing protein 35, TPR repeats / IFT121-like, TPR repeats / : / : / IFT121, second zinc finger domain / IF140/IFT172 TPR domain / : / IFT80/172/WDR35/WDR19 TPR domain / Intraflagellar transport protein 122 homolog / : / : / : / IFT122 second beta-propeller / IFT122 first beta-propeller / IFT122, TPR domain / : / Tetratricopeptide repeat protein 21 forth ARM domain / Ras of Complex, Roc, domain of DAPkinase / Quinoprotein amine dehydrogenase, beta chain-like / Tetratricopeptide repeat / HAT (Half-A-TPR) repeat / Small GTPase Rab domain profile. / HAT (Half-A-TPR) repeats / Tetratricopeptide repeat / Quinoprotein alcohol dehydrogenase-like superfamily / Class I glutamine amidotransferase-like / TPR repeat profile. / Tetratricopeptide repeats / Tetratricopeptide repeat / Rho (Ras homology) subfamily of Ras-like small GTPases / Galactose-binding-like domain superfamily / Ras subfamily of RAS small GTPases / Small GTPase / Ras family / Rab subfamily of small GTPases / Small GTP-binding protein domain / Tetratricopeptide-like helical domain superfamily / WD domain, G-beta repeat / Trp-Asp (WD) repeats profile.
類似検索 - ドメイン・相同性
Uncharacterized protein / Uncharacterized protein / Intraflagellar transport protein 46 / Intraflagellar transport protein 80 / Intraflagellar transport protein 22 / Intraflagellar transport protein 27 / Uncharacterized protein / TRAF3-interacting protein 1 / Uncharacterized protein / Intraflagellar transport protein 121 ...Uncharacterized protein / Uncharacterized protein / Intraflagellar transport protein 46 / Intraflagellar transport protein 80 / Intraflagellar transport protein 22 / Intraflagellar transport protein 27 / Uncharacterized protein / TRAF3-interacting protein 1 / Uncharacterized protein / Intraflagellar transport protein 121 / Intraflagellar transport protein 139 / Intraflagellar transport protein 144 / Intraflagellar transport protein 25 / Intraflagellar transport protein 122 homolog / Intraflagellar transport protein 57 / Intraflagellar transport protein 172 / Intraflagellar transport protein 81 / Intraflagellar transport protein 74 / Intraflagellar transport particle protein IFT20 / Osm-6-like protein
類似検索 - 構成要素
生物種Chlamydomonas reinhardtii (クラミドモナス)
手法サブトモグラム平均法 / クライオ電子顕微鏡法 / 解像度: 15.4 Å
データ登録者Lacey S / Pigino G
資金援助European Union, 2件
OrganizationGrant number国
European Research Council (ERC)European Union
Other government
引用ジャーナル: Cell / 年: 2024
タイトル: Extensive structural rearrangement of intraflagellar transport trains underpins bidirectional cargo transport.
著者: Samuel E Lacey / Andrea Graziadei / Gaia Pigino /
要旨: Bidirectional transport in cilia is carried out by polymers of the IFTA and IFTB protein complexes, called anterograde and retrograde intraflagellar transport (IFT) trains. Anterograde trains deliver ...Bidirectional transport in cilia is carried out by polymers of the IFTA and IFTB protein complexes, called anterograde and retrograde intraflagellar transport (IFT) trains. Anterograde trains deliver cargoes from the cell to the cilium tip, then convert into retrograde trains for cargo export. We set out to understand how the IFT complexes can perform these two directly opposing roles before and after conversion. We use cryoelectron tomography and in situ cross-linking mass spectrometry to determine the structure of retrograde IFT trains and compare it with the known structure of anterograde trains. The retrograde train is a 2-fold symmetric polymer organized around a central thread of IFTA complexes. We conclude that anterograde-to-retrograde remodeling involves global rearrangements of the IFTA/B complexes and requires complete disassembly of the anterograde train. Finally, we describe how conformational changes to cargo-binding sites facilitate unidirectional cargo transport in a bidirectional system.
履歴
登録2024年1月31日-
ヘッダ(付随情報) 公開2024年7月24日-
マップ公開2024年7月24日-
更新2025年8月6日-
現状2025年8月6日処理サイト: PDBe / 状態: 公開

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構造の表示

添付画像

emd_19515.png

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マップ

ファイルダウンロード / ファイル: emd_19515.map.gz / 形式: CCP4 / 大きさ: 66 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
投影像・断面図

画像のコントロール

大きさ
明度
コントラスト
その他
軸Z (Sec.)Y (Row.)X (Col.)
6.06 Å/pix.
x 224 pix.
= 1357.44 Å		6.06 Å/pix.
x 345 pix.
= 2090.7 Å		6.06 Å/pix.
x 224 pix.
= 1357.44 Å

表面

投影像

断面 (1/3)

断面 (1/2)

断面 (2/3)

画像は Spider により作成

これらの図は立方格子座標系で作成されたものです

ボクセルのサイズX=Y=Z: 6.06 Å
密度

ヒストグラム

ヒストグラム (対数)
表面レベル登録者による: 4.5
最小 - 最大0.0 - 11.328854
平均 (標準偏差)0.04508458 (±0.47787383)
対称性空間群: 1
詳細

EMDB XML:

マップ形状
Axis orderXYZ
Origin-30-68-53
サイズ345224224
Spacing224345224
セルA: 1357.44 Å / B: 2090.7 Å / C: 1357.44 Å
α=β=γ: 90.0 °

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添付データ

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試料の構成要素

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全体 : In situ retrograde intraflagellar transport trains in dhc1b-3 mut...

全体名称: In situ retrograde intraflagellar transport trains in dhc1b-3 mutant Chlamydomonas reinhardtii cilia
要素
  • 細胞: In situ retrograde intraflagellar transport trains in dhc1b-3 mutant Chlamydomonas reinhardtii cilia
    • タンパク質・ペプチド: Intraflagellar transport protein 144
    • タンパク質・ペプチド: Intraflagellar transport particle protein 140
    • タンパク質・ペプチド: Intraflagellar transport protein 122 homolog
    • タンパク質・ペプチド: Intraflagellar transport protein 121
    • タンパク質・ペプチド: Intraflagellar transport protein 139
    • タンパク質・ペプチド: IFT88
    • タンパク質・ペプチド: IFT70
    • タンパク質・ペプチド: Osm-6-like protein
    • タンパク質・ペプチド: Intraflagellar transport protein 172
    • タンパク質・ペプチド: Intraflagellar transport protein 80
    • タンパク質・ペプチド: IFT54
    • タンパク質・ペプチド: Intraflagellar transport particle protein IFT20
    • タンパク質・ペプチド: Intraflagellar transport protein 57
    • タンパク質・ペプチド: Clusterin-associated protein 1
    • タンパク質・ペプチド: Intraflagellar transport protein 74
    • タンパク質・ペプチド: Intraflagellar transport protein 81
    • タンパク質・ペプチド: Intraflagellar transport protein 27
    • タンパク質・ペプチド: Intraflagellar transport protein 25
    • タンパク質・ペプチド: Intraflagellar transport protein 46
    • タンパク質・ペプチド: Intraflagellar transport protein 22

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超分子 #1: In situ retrograde intraflagellar transport trains in dhc1b-3 mut...

超分子名称: In situ retrograde intraflagellar transport trains in dhc1b-3 mutant Chlamydomonas reinhardtii cilia
タイプ: cell / ID: 1 / 親要素: 0
含まれる分子: #1-#2, #15-#19, #3, #14, #4-#6, #20, #7-#13
詳細: Temperature sensitive cells incubated at restrictive temperature (34 degrees C) for 10 hours and plunge frozen on grids.
由来(天然)生物種: Chlamydomonas reinhardtii (クラミドモナス)
株: dhc1b-3

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分子 #1: Intraflagellar transport protein 144

分子名称: Intraflagellar transport protein 144 / タイプ: protein_or_peptide / ID: 1 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Chlamydomonas reinhardtii (クラミドモナス)
分子量理論値: 150.972844 KDa
配列文字列: MKKLFGVGPD LLGDGKVLFE WSPKGNFLAA AGSKRKVNIF DRNGRLYDEV HFPPAEYPNP DGRACAAAQM QWDPAGEQLA ILPAGNTHV FIWLAGNKEV QKIESEFKTQ EFSCMAWSRN GMYLGVATVK GNVMMYNARE RKKTPLVGKH TKKIVAAAWN K DNIMALAG ...文字列:
MKKLFGVGPD LLGDGKVLFE WSPKGNFLAA AGSKRKVNIF DRNGRLYDEV HFPPAEYPNP DGRACAAAQM QWDPAGEQLA ILPAGNTHV FIWLAGNKEV QKIESEFKTQ EFSCMAWSRN GMYLGVATVK GNVMMYNARE RKKTPLVGKH TKKIVAAAWN K DNIMALAG QDKTVTLTDG VTGDTIKTFH LKDVPMDLCV SDKKEDGYSR REENTYSLNI NRKTLYIMQC TAEGDRPLEL AF LDTYGPI MKHSWFGDGY ILLGYKNGYV AVVSSHSREI SEEVHSGKYL DTLTDVTYCA SLGRVAMAGA NCVRVLDANA DYN EIKGDA VDLDANQAIE KVGWTKDGQV LTVGTHNGYM HSFLASLPMV YDFHGTRVLY LTSLLEMTLL DVSRRQTVAR IELE NEPAF CGLGPSHAAV GMNNQAAFYS LGEKVGKVVQ RREYLGTITA IKLNETQAAV LTGGHVVVHP ISVEAGHAPD ELDVV IPGP GQPANITCVA LTPTFVITGS RTGTLSYYLS PDVTPVNEFR HDDGGIVRLF PQATGARLVF EDDKGALHLF NPVNDH VVA VPYTGRAETV MWDTSDTNVM VIGDGTALHS FLYVPVSLTG PQVQDLGKQA VPATHTPLTV CNGVVGCRLK SGAMDNV TL ESHKMLQPGD AVARAAPAKR FAAALKLYKL RDAVECAKQL RQVESWRTLA LAALDVLDID TAINAYREIG DASMVLSL E RVRQHEDRNL LSAHIMVLLE KDYGQAQELF LRSSVPRAAL EMRMDLKHWT DALKLAEQLD PDAIATICKE HGAMLEMTG EYSNAKSHYQ QALDALAVSV GPAQPDLEAA CKAGIARTTL QLGDLRQGRQ LAMQLNSQTL FKECALILEG LQQLTEAAEM YERAGQFER AASIYIQTKN FAAAAPLMAR ISSSKLQLQF AKAKEAEGRW QEAAAAYEAA GDMDAVVRLC LERLSQPQRA Y AIVRKTQS VEAANQLSRF CLQSQDFGGA VEFLLMAGQM DQAFDIAMGH NEMDTFARIV AASAKPVDYQ RIAQYYESRG EY DKAADMW SKCDQAPRAV QLYLKVGTNP ALEKAVQVVE QTRSHQLGVL VLDYVNEEKD GTTRDEFRFK LNIAMGQFAE AAR DALEMA RFEQEEGNYR VAHDKLFGTV KQLEALNTKP PGELLRALML LHSYTLVKSL IAINDHTTAA RMLVRVARNI SKFP KHIVP ILTSTVIECH RANLKKTAFE YASMLMRPEY RDQVAVKYKK KIELMVRKPE KDPEELEEPL ADCPFCNMPG PETEL QCIS CQNILPFDLA TGKRMVLSDW AECPGCKFPA SASQFIRIIS AEGRCPMCND PVDLAHVRKV QDPLTKIKQQ QAQQTN ASG GGAS

UniProtKB: Intraflagellar transport protein 144

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分子 #2: Intraflagellar transport particle protein 140

分子名称: Intraflagellar transport particle protein 140 / タイプ: protein_or_peptide / ID: 2 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Chlamydomonas reinhardtii (クラミドモナス)
分子量理論値: 157.426641 KDa
配列文字列: MEIYYTTKIS GRAGVIAGWS KAPGYPSLLA VALSPNAVGI YNEEGRPLDP NNEANIKNVR GTECAKIAWH PLLPLLAIGW KDGAISFWN AEERKLEEDS KIHRNTISSM TWTASGDRLI TGDENGRISM WKTDRLMRPI HVVAYDEPGA VIRHTVVGLP E EMPDTNSQ ...文字列:
MEIYYTTKIS GRAGVIAGWS KAPGYPSLLA VALSPNAVGI YNEEGRPLDP NNEANIKNVR GTECAKIAWH PLLPLLAIGW KDGAISFWN AEERKLEEDS KIHRNTISSM TWTASGDRLI TGDENGRISM WKTDRLMRPI HVVAYDEPGA VIRHTVVGLP E EMPDTNSQ VVVAFYVADA QERAVVKWCN DQGYSGVVVE MSEGVHTLVH YPERDQLLVV GSSCTLNVLT RDEQLGTWVT AS KMKFATG TGEAATGLQV AWAGNHTLAS ASEKDNVVRM YNFDTEDNYV LNLEQDSGLV SRVVCLAYDD RYNLLAVGTT DGR VMMYKF NQPNLNLEPV LDFAKCWEMQ PAFFVGNRAL AMEWGPFPRL MVVACNDAIN VCRKTMLSYK FRDGVAIMQV AVDR VVLEN LEVEPQRPPG RLQLQDMQLL GLDLSKGLLL VWDGERAEVY KVTETNDIMP ASQFETTSRC MAINNDSVYR AAENK VEVV NMAGTVKQTL MFDDNHGSPT SMDVARDYLA VVTSANIVRI LKVAGREAKP HAGPAPLCPP ELKDWKVMAM RANGNG NLV AALVVGPEPA RAVRLVVWCG ETNTHHVHDF TPEGRQPVQL MFDTVEPKLL VVQCTPVFTE ANVDERPHGD AAHVVDC AI IFVDPDKGIL LQEYQPIHTG GATACIGSCA PHLLTNKKSM VQPAPGSGAF QPFTSNVSKA IMTSFQGMQD SDDKTRRA L LDFSFNLATG NMDEAFRSVK AIKNPAVWEN MAHMCIRNKR LDVAEHCLSN MEHARGARAL REAKSIEEAD ARVATVAVH LGMIEDAKKL YIACERYDLL NQLYRACGQW DKALEVAEKN DRIHLKSTHY AYGQFMERQG DMEGARKHYE AAGCGLVEVP RMLFEAEKF TELQNYIQAN DSRELILWWG KYLESLGEYA KALDCYRKAG DSLSMVRIHC FQRDWKAAED EVTNSADNAA S FHLARQYE ASGRIPEAIR YYTLAKRYSH GVRLAKTHEL DSDLMNLALK STPAVMIDTA DYLFAKGQHE KAATLYMKGG KL SKAVEMC FQAQLFDVLQ HITDDMTPEK SDPNLYNKCA EFFMGFGHND KAVKMLIAAQ QYGRALELCV EHDVSITEEM ADS MTPDKN AAVSADERNN VICRIAKVAK RQGNFQLAAK KYTQAGDKVK AMKALLRGGD AEKIIFFAGV SRQKDIYLMA ANYL QTLNW HSDPELMKHI ISFYTKAAAW ESLASFYEAC AQIEVDEYRD YEKALQAMRE AAKYVAKSKN DDRDARVGVI NDRIA VAEQ FVAARQLIGS NPQEALRVCD ELLRAIPPNS QDLEAGIRIG DVYALMVEYW YEARNPNEAY KAIEAMRRRG IILSPY LDT RMVEDIYRSL GVALDMAEER RGPANLGLRE SDAGAFVEEE VADEDD

UniProtKB: Uncharacterized protein

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分子 #3: Osm-6-like protein

分子名称: Osm-6-like protein / タイプ: protein_or_peptide / ID: 3 / コピー数: 2 / 光学異性体: LEVO
由来(天然)生物種: Chlamydomonas reinhardtii (クラミドモナス)
分子量理論値: 50.453184 KDa
配列文字列: MEEPGAEEVR ILFSTAKGES HTHKAGFKQL FRRLRSTYRP DKVDKDDFTL DTLRSAHILV LGGPKEKFTA PEVDMLKKFV KNGGSILIL MSEGGEEKAG TNINYFLEQF GMSVNNDAVV RTTHYKYLHP KEVLISDGIL NRAVITGAGK SLNSNDDDEF R VSRGPQAF ...文字列:
MEEPGAEEVR ILFSTAKGES HTHKAGFKQL FRRLRSTYRP DKVDKDDFTL DTLRSAHILV LGGPKEKFTA PEVDMLKKFV KNGGSILIL MSEGGEEKAG TNINYFLEQF GMSVNNDAVV RTTHYKYLHP KEVLISDGIL NRAVITGAGK SLNSNDDDEF R VSRGPQAF DGTGLEYVFP FGATLSVQKP AVPVLSSGKI AYPMNRPVGA VWAQPGYGRI AVLGSCAMFD DKWLDKEENS KI MDFFFKF LKPHSKIQLN DIDAEEPDVS DLKLLPDTAS LADKLKGCLQ EIDDVPRDWT SLFDDSLFKF DTGLIPEAVS LYE KLGVKK GQLNLIPPSF ETPLPPLQPA VFPPTIREPP PPALELFDLD ESFASETNRL ASLTNKCHGE EDLEYYIMEA GHIL GLKLQ ENANAKHVLS EVFRRIAQYK MGSLGLGQTL DSMGQTLPAA NQFGDQFEL

UniProtKB: Osm-6-like protein

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分子 #4: Intraflagellar transport protein 80

分子名称: Intraflagellar transport protein 80 / タイプ: protein_or_peptide / ID: 4 / コピー数: 2 / 光学異性体: LEVO
由来(天然)生物種: Chlamydomonas reinhardtii (クラミドモナス)
分子量理論値: 85.782609 KDa
配列文字列: MRLKVKQSSA NVHSELTAAV GWNVWNELFT CSDDQTIHKW NMLGEPEQKV STLDAYFTDM HWYPVSSKKT QAGGTDVFAV ACTDGSVKI LSRTGRVEKS IEGHKGACIS LRWSYDGTAL ATAGEDGSVK IWSRNGMLRS TLAQADSPVY SIVWAYDCDQ L CYCTGSNV ...文字列:
MRLKVKQSSA NVHSELTAAV GWNVWNELFT CSDDQTIHKW NMLGEPEQKV STLDAYFTDM HWYPVSSKKT QAGGTDVFAV ACTDGSVKI LSRTGRVEKS IEGHKGACIS LRWSYDGTAL ATAGEDGSVK IWSRNGMLRS TLAQADSPVY SIVWAYDCDQ L CYCTGSNV VIKSLSSNAK QNAWKAHDGV VLKVDWSPIN HLIITGGEDC KYKVWDSFGR LLFQSGLFDY PVTSVAWAPS GE LFAVGGF NTLQLCDRMG WAYSKIHLND TGSIMTLSWT ADSTQLAGGG GSGGVVFGQV VDLALEDGKM QVTVVDDMRI VVN DILNEN ADELPEFRDR VIKVSLGYGY LIVATATQCH VYNTTNLGTP HIFDLKDTVT LLLQAERHFL LLDNSAGIQI YTYE GRQIC NPRFQGLRTE LLNAQMITLS NDTIAVLDQQ ASGTTVRFFD TAQGRPVGEP WQHTLEVKEI ALSQAGTIND RQLIV IDRN RDLYLLPVMK RHVAKLAAMC DSARWHDSTA MLSAMVDQRL CVWYYPSEVY VDKDLLAKTR YTKSDSDFGK SAQIQL FAG NRCLVRRSDG VLVSAATSPY PAVLYDMIRK QQWDKATRLC RFIKDPTMWA TLAAMAMAAK ELNTAEVAFA AIDEVDK TH FVRKVKQIPT EEGRNAELAV YRRKPEEGES ILLQAGLVFR AIKLNIKLFN WERALDLATQ HKQHQDTVLW YRQQFLKN A KLAESITRFM QMNESVVVDQ AAVKKKIEEE RIKESQRPGA KRYV

UniProtKB: Intraflagellar transport protein 80

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分子 #5: IFT54

分子名称: IFT54 / タイプ: protein_or_peptide / ID: 5 / コピー数: 2 / 光学異性体: LEVO
由来(天然)生物種: Chlamydomonas reinhardtii (クラミドモナス)
分子量理論値: 54.437715 KDa
配列文字列: MCDNWQATID TLQGASPVFD KPKLSQKLLE KPPFRFLHDV VTAVQQATGF APGLYQGDEL DGKAIQEKDA KVAYLKKIIE VVSMVLGEQ CPARPNKIVA GLEPENTNIF LQMLGRACQK GNGAKAVQKV LGGGGAEPAP AKEEAPPPEK KPEKKEKKEE K PAEKSRAE ...文字列:
MCDNWQATID TLQGASPVFD KPKLSQKLLE KPPFRFLHDV VTAVQQATGF APGLYQGDEL DGKAIQEKDA KVAYLKKIIE VVSMVLGEQ CPARPNKIVA GLEPENTNIF LQMLGRACQK GNGAKAVQKV LGGGGAEPAP AKEEAPPPEK KPEKKEKKEE K PAEKSRAE ASPARKKAAE PEAEKKSSSK SSSRTKEEPP AKAPAKKKEE PAPEKPSKSK AAPAAEEAPP PPPPPAAEPP AR SASPGGE DPLNKSGSAA PKFQRPTSAR KAPPRVPQPQ QPTMLAGTGI RPGTATRRPN EPKPTDSKVT KPVAVFTDNA KDN SDDEVE VVHEQTPVLS GGANMTGEQG VLVKDILAAE KGLKKAGVDA TADNADTSDQ GSTGIILKRL GGKAAGAGAA AAGP RAHDP SSVRELVQKL CHSSTPLAKS MDYLQEDIEN MRKEYKFWLT EKRMYQDELA RELRLQGEAA NVDAQLADLD GQIKQ ARDR IIGMKGQILR NDETLGKLLA MATAGR

UniProtKB: TRAF3-interacting protein 1

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分子 #6: Intraflagellar transport particle protein IFT20

分子名称: Intraflagellar transport particle protein IFT20 / タイプ: protein_or_peptide / ID: 6 / コピー数: 2 / 光学異性体: LEVO
由来(天然)生物種: Chlamydomonas reinhardtii (クラミドモナス)
分子量理論値: 15.658664 KDa
配列文字列:
MDAVDRGVYF DEDFHVRILD VDKYNASKSL QDNTNVFINN IQNMQGLVDK YVSAIDQQVE RLEAEKLKAI GLRNRVAALS EERKRKQKE QERMLAEKQE ELERLQMEEQ SLIKVKGEQE LMIQKLSDSS SGAAYV

UniProtKB: Intraflagellar transport particle protein IFT20

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分子 #7: Clusterin-associated protein 1

分子名称: Clusterin-associated protein 1 / タイプ: protein_or_peptide / ID: 7 / コピー数: 2 / 光学異性体: LEVO
由来(天然)生物種: Chlamydomonas reinhardtii (クラミドモナス)
分子量理論値: 49.733469 KDa
配列文字列: MSFRELRSFT EVMKALGYPR LISMENFRVP NFELVADCLY WLVHRYYPGV EIADDISTES DRVKFLQSVA QVMLTKARMK LNIKRLYAA DGNAVKELLK LASLLYKATS KAGDMDDDAA EAIDVANSLK GFNPKEIKGL AAEIIKAGAG LYDALGQEPE L REHRARAV ...文字列:
MSFRELRSFT EVMKALGYPR LISMENFRVP NFELVADCLY WLVHRYYPGV EIADDISTES DRVKFLQSVA QVMLTKARMK LNIKRLYAA DGNAVKELLK LASLLYKATS KAGDMDDDAA EAIDVANSLK GFNPKEIKGL AAEIIKAGAG LYDALGQEPE L REHRARAV AGHVDTDFVE RSIREAIAQV EDNIRSLENQ MEDLERNEKT LDNKIEKKKQ ELERQEKRLS TLQSVRPAYM DE YERLQGE LNGLYQSYLD RFRNLEFLEN ELEAYYQAEQ EKMEAQERRL KKMQKRLKEE ELRILRGEQE VDEANMNFDD DDM DLSEDG EHEEAEEGED EDYGAGGQKA TMRRDQFGGQ GGGGGGGRQQ GGGRNVMGNL DGDFDDEASI DEDDNTDGGE VSRQ GSQAG GMGGGGGYGG GGGYMNGGGM DDDENNLIQD DDEDDNDF

UniProtKB: Uncharacterized protein

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分子 #8: Intraflagellar transport protein 74

分子名称: Intraflagellar transport protein 74 / タイプ: protein_or_peptide / ID: 8 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Chlamydomonas reinhardtii (クラミドモナス)
分子量理論値: 71.648398 KDa
配列文字列: MDRPSSRGAL ALGAGGLGKA PTGGAVQQPD RPMTGQRGAA PAGPMRAPAG ASIIGAGPPG TAMRGGPGPA GGPPGTAYKR MGTASQRPG TGQQAAAAAA AARAGQQLQV ENRPITNHGV SGMKTAAAGV GRQVLDKNYF MNELRQKRME IAQVTSNMKS D LEALERKQ ...文字列:
MDRPSSRGAL ALGAGGLGKA PTGGAVQQPD RPMTGQRGAA PAGPMRAPAG ASIIGAGPPG TAMRGGPGPA GGPPGTAYKR MGTASQRPG TGQQAAAAAA AARAGQQLQV ENRPITNHGV SGMKTAAAGV GRQVLDKNYF MNELRQKRME IAQVTSNMKS D LEALERKQ AQYNSMDKRA SDLSKEVKIL QEALADYNTV LDKVGSQAPV YVIQQEFAAL KDRNEQQRKR VDEVLTERLN LE SKAKQAE SKMSEIQASM DQRLNSMPPS QRNEYTTLVA EQQQLQADSK RFEEVLDELD KALQASEGEL ARNPFKQRSL QLQ EQIRAL TEKKYELTEE ERQSKRSPEE LRADLMAKIK RDNTEVEQMT QQIRELQDQI KKMEERVKSL GGATSGAAAA EEKA NREKF EELLAKERDL NNFMDGFPSR KAAKMQEKQQ KEDGIVGVLE KMVKMQGIIG SNLPSQKKYK EMQDELEYKK MQLEN TQTT QERLKEELTM RRTELEKIDT LEDKIKLELT QLAERQEAME KEMGEFGSVE DIQRKANAAR ERMEGLRSVL LKRKDL LRS IVAERGLKFQ AKRAQLQDHN LQVQLEKMEA KLKNLSAGVF EMDEFIKAKE SETNYRQLAS NIAALVDDLN VHVKKAV V

UniProtKB: Intraflagellar transport protein 74

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分子 #9: Intraflagellar transport protein 81

分子名称: Intraflagellar transport protein 81 / タイプ: protein_or_peptide / ID: 9 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Chlamydomonas reinhardtii (クラミドモナス)
分子量理論値: 77.186484 KDa
配列文字列: MGDVSYIVDS LGLPPFSYQM SLLSFTEKGP QELLQLLSDV FSTISPKHQK VDVAKEVPDQ TADRLIGFLK IIKYRPNVQD PLLFRQLVA AGDRETLYQI LRWVVPQAQL LEKRAFVGYY LSFPDMPEEF NFDPDIMELK EEIKAMQQEF IELHKSSDAI K TLSKDTQA ...文字列:
MGDVSYIVDS LGLPPFSYQM SLLSFTEKGP QELLQLLSDV FSTISPKHQK VDVAKEVPDQ TADRLIGFLK IIKYRPNVQD PLLFRQLVA AGDRETLYQI LRWVVPQAQL LEKRAFVGYY LSFPDMPEEF NFDPDIMELK EEIKAMQQEF IELHKSSDAI K TLSKDTQA LKNKIKSLEE EKERLGEKVE RAKGAVDKLP DRSSYMEVCT NLRKQQDEEV NLSTAIQTQR ALQEKAEASY HR AAARLRE LQTSYQEGSA GKLLETLNED VKNLRAQVNE RYPKEVEKRQ KRQAALSEAL ASGISTEMDL QRLQHQATAL HNQ ITEIQE RKVAQDKARQ GDKAYLQLRQ AQQMATVSAR KKEELGAKQE RLQEKKTALT AQLDKLNAEG GGSGAVFSEE EWRT KYESM KSKLPIYKKM KKELGDLEAE VFVLAHTEEL LASQEGGLLE KVKRLEKQQG ISGFTETAQH LEKVSEAKSQ MDEEK GMTL IEISRTVEEI NNAINQRKQQ LAPQIKKLRS VRQDFAEFEA KYLEKKTAYD NVVATFEART SALEGEVSGL KAEVSE NET KYHMLHCQLH ITDQNIKKVT SGPAAERLRD KYEAKVKEAE DSTKALRDRQ REIKDTHSTG LSQIDIMNDM LRLLQLK LN LARGIAVDMS QYGGGGGAAG GANGGMAGQT YDTGSANVLQ L

UniProtKB: Intraflagellar transport protein 81

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分子 #10: Intraflagellar transport protein 27

分子名称: Intraflagellar transport protein 27 / タイプ: protein_or_peptide / ID: 10 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Chlamydomonas reinhardtii (クラミドモナス)
分子量理論値: 22.783967 KDa
配列文字列: MVKKEVKPID ITATLRCKVA VVGEATVGKS ALISMFTSKG SKFLKDYAMT SGVEVVVAPV TIPDTTVSVE LFLLDTAGSD LYKEQISQY WNGVYYAILV FDVSSMESFE SCKAWFELLK SARPDRERPL RAVLVANKTD LPPQRHQVRL DMAQDWATTN T LDFFDVSA ...文字列:
MVKKEVKPID ITATLRCKVA VVGEATVGKS ALISMFTSKG SKFLKDYAMT SGVEVVVAPV TIPDTTVSVE LFLLDTAGSD LYKEQISQY WNGVYYAILV FDVSSMESFE SCKAWFELLK SARPDRERPL RAVLVANKTD LPPQRHQVRL DMAQDWATTN T LDFFDVSA NPPGKDADAP FLSIATTFYR NYEDKVAAFQ DACRNY

UniProtKB: Intraflagellar transport protein 27

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分子 #11: Intraflagellar transport protein 25

分子名称: Intraflagellar transport protein 25 / タイプ: protein_or_peptide / ID: 11 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Chlamydomonas reinhardtii (クラミドモナス)
分子量理論値: 20.380588 KDa
配列文字列: MKDYAREENG GLVVMASCSD ERFPPENMLD GKDNTFWVTT GMFPQEFVLR LESCIRVSKI TTLSLNVRKL AVEKCDQDKP DQFEKVFEV ELANRGDRLQ TEVHQVNIRA KYLKFILLQG HGEFATVNRV SVVGGDDDGG GYDEPGGGYG SMQRQPSMGY G GGGGSAAT ...文字列:
MKDYAREENG GLVVMASCSD ERFPPENMLD GKDNTFWVTT GMFPQEFVLR LESCIRVSKI TTLSLNVRKL AVEKCDQDKP DQFEKVFEV ELANRGDRLQ TEVHQVNIRA KYLKFILLQG HGEFATVNRV SVVGGDDDGG GYDEPGGGYG SMQRQPSMGY G GGGGSAAT GFAADPGNAA AGGGGGFEDE F

UniProtKB: Intraflagellar transport protein 25

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分子 #12: Intraflagellar transport protein 46

分子名称: Intraflagellar transport protein 46 / タイプ: protein_or_peptide / ID: 12 / コピー数: 2 / 光学異性体: LEVO
由来(天然)生物種: Chlamydomonas reinhardtii (クラミドモナス)
分子量理論値: 37.97641 KDa
配列文字列: MDDSMDYPDR DGDDLDQFQG TARSQVVQNQ PHDEEVNLSE SESFAGADEP PAAPRDASLI ESHDMDEGPA APARTLSPTG YEAGKHAPG GIANSDEAPP GAYNAQEYKH LNVGEDVREL FSYIGRYKPQ TVELDTRIKP FIPDYIPAVG GIDEFIKVPR P DTKPDYLG ...文字列:
MDDSMDYPDR DGDDLDQFQG TARSQVVQNQ PHDEEVNLSE SESFAGADEP PAAPRDASLI ESHDMDEGPA APARTLSPTG YEAGKHAPG GIANSDEAPP GAYNAQEYKH LNVGEDVREL FSYIGRYKPQ TVELDTRIKP FIPDYIPAVG GIDEFIKVPR P DTKPDYLG LKVLDEPAAK QSDPTVLTLQ LRQLSKEAPG AKADMVGRLE HTDENKAKKI QQWIASINDI HKAKPAATVN YS KRMPEIE ALMQEWPPEV ETFLKTMHMP SGDVELDIKT YARLVCTLLD IPVYDDPVES LHVLFTLYLE FKNNPIFRQH MEM ENKLDG MSGGGGGMMG GGADVLGL

UniProtKB: Intraflagellar transport protein 46

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分子 #13: Intraflagellar transport protein 22

分子名称: Intraflagellar transport protein 22 / タイプ: protein_or_peptide / ID: 13 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Chlamydomonas reinhardtii (クラミドモナス)
分子量理論値: 21.363285 KDa
配列文字列: MAEHGLKIAV VGPQRTGKTL LCRALAEQPI LLGEMSYQPT AAVRIQEISR VLGIDRVKVQ FWDVSGSVQY QSYWPVLAKE VDGLLMVID PNRPEQERDL ETFYRNFAEP NNLYTRQCMV MAIQVQKEGG GLGGWQGLQG GLKKLSQSYV AINPANPAAG V QEAYSHLD ...文字列:
MAEHGLKIAV VGPQRTGKTL LCRALAEQPI LLGEMSYQPT AAVRIQEISR VLGIDRVKVQ FWDVSGSVQY QSYWPVLAKE VDGLLMVID PNRPEQERDL ETFYRNFAEP NNLYTRQCMV MAIQVQKEGG GLGGWQGLQG GLKKLSQSYV AINPANPAAG V QEAYSHLD VLFQGALQSK KEALESSYMN QEDQ

UniProtKB: Intraflagellar transport protein 22

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分子 #14: Intraflagellar transport protein 172

分子名称: Intraflagellar transport protein 172 / タイプ: protein_or_peptide / ID: 14 / コピー数: 2 / 光学異性体: LEVO
由来(天然)生物種: Chlamydomonas reinhardtii (クラミドモナス)
分子量理論値: 197.851172 KDa
配列文字列: MQLRYFKSIL PPADQYQKIT SLTWAPNNSR LAAVSTDKVV YLFDENGEKR DKFKTKAAEA NNPNTYIIRA MAFSPDSTKL AIAQSDNIV FIYRLVDPDT GAEKKSICNK FPQACAVTSL VWPKDRPNEV VFGLADGKVR LGMLKNNKSY TCYAHPENSY V VALASSLN ...文字列:
MQLRYFKSIL PPADQYQKIT SLTWAPNNSR LAAVSTDKVV YLFDENGEKR DKFKTKAAEA NNPNTYIIRA MAFSPDSTKL AIAQSDNIV FIYRLVDPDT GAEKKSICNK FPQACAVTSL VWPKDRPNEV VFGLADGKVR LGMLKNNKSY TCYAHPENSY V VALASSLN GQNVISGHMD GAIWKFNFPA EEGGTPTSSQ LVVHSCVPYS LGWGSCIAAA GNDNRVVFYD LNGREIRSFD YS NNDEVRE FTTCAFNPSG DTVVFGTYNR FYMYTFNIQR NDWEEAGHKQ IDNFYAVSAA SWKPDGSKMT VGSMTGAVDM YDA CVKRHM YKGKFEFTYV SKSAVIVKTL KTGMRIVLKS VYGYEIEKIN IYHDRYLIAR TTYTLLMGDL DTCKLSEIPW DSDG SEKFH FENERVCMVH YAGELHIVEY GRNDVLGTCR TEHMNPYLIS AVVQEARGIA SESKKLAYLI DLQTVRIQDL MAPVG STLA TVNHDTKVDW LELNQRGTHL LFRDKKRHLH LFSLSGQERT TLLNYCQYVQ WVPGSDVIVA QSRNNLCVWY SVNKPD NVT MFPIKGEVVD IERHNHRTEV IVDEGINTVS YALDEALIYF GAALEDQDYE RAVQTLEPLE LTPETEAQWM QLAEQAL AT NQLVIAERCY AALGDIAKSR FLHKVVKKAQ QAAKEFGGDG TDAWSVRAMM AQLNKQWPVS ESLLLAQGKV DDAITLYQ D NHRWEDAIRV ADSTHHANAA ALKQQYLTWL LETGQEEQAG AVKEREGDYL AAIGLYLKGG LPGRAAQVVM SVHNVNWDP ALLDSILASL AKAGLYERAG ELYEHMSRSS EAMQSYRRGH AYRKAIDLAR REFPAEVIII EEEWGDWLVT QKQMDAAINH FIESGATLK AIKAAIDCRQ FAKAAGIIEV LDPREAMPYF RRIAQHYETT GALEEAERYY IRADMARDAV EMYSRAGKWE A AQRVARGY LTESEMRAFY RAKAAEFEAA HKLKEAEKAY LAAGGDDVDK AIAMYKRNKM YDQMIRLVTQ YRKEKVPEAH TL IAQQLEV EGNLREAEKH FVEAKDWKSA VQMYRQVNQW EDALRVAKVY GGVNASKQVA YAWALTLGGD DGAQLLKKMG LLD HAIEYA VESGAFAQAF EMTRAGAKHK LPEVHLKYAM FLEDEGRFAE AEAEFISAGK PKEACDMYMH NQDWDAAMRI AERY DPTMV SEILVSQARV AVERKQWLPA EGLFIKAKRP EAALKMYRDA RMWNDALRVA EQYLPTKVAE VQMELLSGQG AGGGS GGAS ADAVINKARG FERNNDYARA IETYLSLTAQ DTSNQDQLEH CWGQAAQLAI NYQRHRMKDV VNTVSERLQE IGRHQA AGE LHESIDDAQG AIRAYCAGRL WDKARTLAGT NPTFSRYIED QYNNYLLQNQ QADELASRGG QHAQQAIEMY VARDEWA KV HELAAQQGPE VASNYALKHA ERRFKQGDYA QAAQVFAQHG ITAQPQYFEL YKSIAQGVLH ASQGDRNPVA EKSLRDMM Y RLVNVLRSGG GAGKYKVDTD AFQNYYLAAH YLTCAAAAKE QGLKDIAAMN LTSVLRYVGP TIPADRAFYE AGLAWYEAG RKNMAFVMLN RFLDLSDAMD EPDSSAAVIE NADFSDTDIP YDFTIPERAY CTESQREDVR NLVLEISMDR SSDQSLALKA CEHCGKPTY EANLTCHFCK KKYDPCVVTG YPIQSYDRVV FKNNGPELNA IRDMWNKWVE AFGTDPVTGM QAAPMY

UniProtKB: Intraflagellar transport protein 172

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分子 #15: Intraflagellar transport protein 122 homolog

分子名称: Intraflagellar transport protein 122 homolog / タイプ: protein_or_peptide / ID: 15 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Chlamydomonas reinhardtii (クラミドモナス)
分子量理論値: 139.382922 KDa
配列文字列: MRTVVAWQET PPEKDGVRNV CYDLCFKPDG SQVVAGIGNR VLVYDAADGD LLHALKGHKD SIYCVAYAQN GKRFASGGAD KTVIIWTSK AEGILKYTHN DAIQCLSYNP VTQQLASATG SDVGLWSPEQ KSVAKHKVAS RICSISWTAD GAYLALGCFD G VISIRDKG ...文字列:
MRTVVAWQET PPEKDGVRNV CYDLCFKPDG SQVVAGIGNR VLVYDAADGD LLHALKGHKD SIYCVAYAQN GKRFASGGAD KTVIIWTSK AEGILKYTHN DAIQCLSYNP VTQQLASATG SDVGLWSPEQ KSVAKHKVAS RICSISWTAD GAYLALGCFD G VISIRDKG GSEKHRIETG PSPVWSICWN PVETNVLCAG CFDGVLKFYM MSGQQKSKDR ELAFDPLCVS YFSSGEYITV SG TDKTVHL YTRDGTYLTK IADRDSWVWA VRPRPKHNFV AVGTEGGGIA MFQLIFSTVH GLYQDRYAYR DQMTDVIIQH LIT EQKVRI KCKDYVKKIA VYKDKLAVQL QNKVVIYELA NADDFDMHYQ SATKIQQKLD CNLLVVTSHH VILCQEKKLQ LYNF DGVKE REWVLDSVIR YIKVVGGPPR REGLLVGLKS GAILKIFVDN PFPIPLIKHT ASVRCLDLSA SRNKLAVVDE NAKVL VYNL ITKELVFEES NANSVAWNSE FEDMFCYSGN GMLSIKTGDF PLHQQKLQGF VVGFKGSKIF CLHYVSMQTI DVPQSA SMY RYLERKDFES AYRVACLGVT EADWKQLALE ALQALNLEVA RKAFIRIRDV RFVELVNRTE AGRKAGTSEQ LLLAEIM AF QGRYQEAARL FTQAGAVDRA MEMFSDLRQF DEAKKWAEEF AASGRGDQRS VQELINRQAE WSEEVKNYDA AAEMYIKA K KYDRAIAILA KHQWWDKLIG VVRQLDKTDA RCLGMCAGHF RRAPHFAYAK ETLLKMDDTK GLITLYVEAE KWDDAFLLL HAHPECRQDV YLPYAKWLSN QDRFDEARLA YQEGGFPSLA TRILEQLCAN AVVETRYADA AFYYYQLAME ALKSIKNPPS NMAPSDRSA LERFTELYDR AEVYYAYEVV HKSVHSPFRT THPDTLFNAS RFLLMRLLPP REVPLGVSVV NVVYVLAKQA V EAGAFKLA RFAYNKLQTL VLPAAWQAEV DLASVVIRSK PFSDKEDLLP VCWRCSTTNP LLNTQGDYCI NCGAPFIRSF VT FEHLPVV EFELEPGVDD EEAGRLLGED AGMEAARRER KAERQAKAAE VGGNMLRLDQ NEIDRMDDAF AAQMMVPNTT IRV DRAMLR RLKTAEVMVR TWPNPVIPKQ YFRVMDQEVP LCCGPCGHFF EQDEFEMAAL ERGTAPFSRT TVRGEGLAPG EDAE DEGAG GNKLGGPLGS ARGPIGGASK ARMSVPFQQG RPLV

UniProtKB: Intraflagellar transport protein 122 homolog

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分子 #16: Intraflagellar transport protein 121

分子名称: Intraflagellar transport protein 121 / タイプ: protein_or_peptide / ID: 16 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Chlamydomonas reinhardtii (クラミドモナス)
分子量理論値: 136.267359 KDa
配列文字列: MFIYLSKKIA IPNNVKLRCI SWNTDQGWIA CGGESGLLKV LRLDGTTSKD VKGKKEGGAP GGNLSLNQTL EGHNGAVICV CWNNAFQKL TTSDEFGLII VWMLHKGMWF EEMINNRNKS VVKDMKWTSN GEKICIVYED GAVIVGSVDG NRLWGKELGM D LALVEWSP ...文字列:
MFIYLSKKIA IPNNVKLRCI SWNTDQGWIA CGGESGLLKV LRLDGTTSKD VKGKKEGGAP GGNLSLNQTL EGHNGAVICV CWNNAFQKL TTSDEFGLII VWMLHKGMWF EEMINNRNKS VVKDMKWTSN GEKICIVYED GAVIVGSVDG NRLWGKELGM D LALVEWSP DGRLLLFATP GGECHVYDGN GNGVAKVPLY CNEGYAGASK IIGVEWYDGV EGYAEPNCPV LAICLDNGRM QL MRYESDD NAVCIDTGIK PVKVKWNMNG TILAVAGFQM SSAVADGREL WMVQFYNHNG EHLRTLRVPG GGISGISWEG NGL RLALSV DSYVYFANVR PDYKWGYFCN TLVYAFNRPD RSEHCVMFWD TKNNDKYPKY VRKLVAIQAF GDFCVLATKG ENPG EHILI LCNAIGSPVD SKYIEVEPKY LAITNYHVIA ANDEVVYVWQ FRTSFSKVLS TDINAVKRKD VREKMFSVDD SNPAL SDRS PESFRNMTTQ TTDPIACVTA CDSVLMIGRA SGVVNRYSLP HLTLDAQHVL RCRPQMLALN CNLSKMSIID INGVLT FFD LTAKAPGGGA NTMGEHLSFE RKDAWDMRWA DDNPELFAMM EKTRMYIFRG LDPEEPVTSS AYLCSFHDLE ITAAFLD DI MQQPDQPDLE FMVMYETRSL RDTRELLKSA SVDDAYAFVD SNSHPRLWRN LAEHALEQLD FTTADKAFVR CADYQGIQ F VKHLGKLDDK AKQRAEVAVY FKRFDEAEQL YMRMDRPDLA IDMRMRLGDW FKVERLIRES SGDDAQLLNA HNKIGQYYS DRHKWGKAAQ YYAQAKNSEM LVECFYALED FVALGRLMDA LPDGSPLLAN IGEKFQSVGL CNEGVTAFLK AGDTKRAIDC CVLLNQWDQ AVQLAQAHNF PQIEQLLAKY ANHLLEKEKV MDAIELYRKA NHSMEAARLL QDLAKKCADQ KVHPLRVKKL Y VLAALEIE KFKKRTLDMA GPDKTAATLL GTGGTATMAA TAAQTLAGLM TLESAVSGEA GVDSAWRGAE AYHFWLLAHR QL YAGNVDL AMRTALHLRE YEDLLDPVEI YSFLALAAFY NQFFGQCSKA FIKLESMPSI PNDKRESFAD LAMSIFLKHP PAD PRALRE TREKKAPGGG AMAGNSLDAL LEDLGGGREQ VCVASGRIVR DGNVVRCKVC KHLSITHELH GAAVCPLCHA ALPV SGGGA PGRGMSAKGG AMAQMYGAY

UniProtKB: Intraflagellar transport protein 121

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分子 #17: Intraflagellar transport protein 139

分子名称: Intraflagellar transport protein 139 / タイプ: protein_or_peptide / ID: 17 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Chlamydomonas reinhardtii (クラミドモナス)
分子量理論値: 152.227688 KDa
配列文字列: MADRVLALVH YYAREGYFRH VQTVCNEVLK KRPGDGVLTF WRAYGLLMEG NTADAMRDLS SIQGNSDLEL AVAAAQLLGH ESAKVPDHD AIIDLQAKLE IEERTASDQP CLHLASFYLY TKSKERARGL VERVLRNQPD MVPAQVLLGW IIISQQQDDE Y DMLFDESE ...文字列:
MADRVLALVH YYAREGYFRH VQTVCNEVLK KRPGDGVLTF WRAYGLLMEG NTADAMRDLS SIQGNSDLEL AVAAAQLLGH ESAKVPDHD AIIDLQAKLE IEERTASDQP CLHLASFYLY TKSKERARGL VERVLRNQPD MVPAQVLLGW IIISQQQDDE Y DMLFDESE LDDALSHFEQ AVEHDHNDLQ ALLGKAKIME LKKQLGPCLD VLTEINVRFG WFVPALVEKT RMLMMLGDWE QV TETLQRV LAADQQNIMA QAWNCMISLT REGNNKQAAK QLQDLFSSMN RQEPKNAELF FRVARPFGRL ACSDPTLLGI TYL MADRAA QLRPEMAAYV VEAAAQKLMM DETTNATERF TQALQLDELN LEANAGALEA QIMAGELEEA AGQIMFLEDM FTNA AAAGG GKRKGRGTGD MDDDPDMADP SLGTSSDNPT LLYLKGLLAW KQGMPSEGLG LLERSIAALF SAAADFHGPS LELYA ALNP ARITAVVRLL LQSIGGEPRA PTEAPSPLIS KVTRALDLLN KQAPALQESA LLHARALYLN GNLDGALRKA GEILRM NPE ESSAHLLICS VYVAQDKPEL AVSALDQAVS SNFAIRETPL YHVVQAKVLV ANNKLDDAKR VLESAMNLPG VRTALTV QQ RARLGRKVVE PTLHERATVY LLLADVLARQ SKIPDAPEAK KYIQDAIREF EGTSEEVRVT VADCELAIAR GDVEGALK K LRRIPKESPH YVKARMAMAD IYLRHRKDKA AYIKCYMDLV DHTPDYDSYC MLGEAFMQIQ EPEKAVRAFE SALEFSPKD VDLITRCARA LVTSHDYQRA IDYYTKAIAN ARGGAQHALQ LELGNLLVRL RQYPAATAAI NKALERNRDG LPATENLQLD VEAWSMLAK VHKGKLEMEG YSQAQSRALE LQKQLLLKLR GELPEAVALQ RERTAAICFD LAEQSKRARQ FDRAMELYME A LRHHDTHV PSMLAVAKLH LANGDTDACQ AQCVTLLKHD PDNEEASIML AELMFHKEHY DTAIYHFQQL LERSPNHYGA LA QLILLLR RAGRLEDVPR YFALAEAGSP KAVMDPGYHY CKGIYNRYIN NPREALKELN LARKDTRWGS QAILHMVEIY LNP DNDAVW EEKENADTPE SRQAVATARS LLKQVRGADT TTQRYRVLES YAIMAGKDKN EIEASLNVLL DLANQDPNNV PVLL AMATG FMMLKQTPKA RNQLKRVQKI QYKPDEAEEF ERSWLLLADI HIQGGKYDLA QDLCQKCLKY NKSCAKAWEI MGQIM EREQ AYKDAADHYE NAWKHENQAS AQVGFKLAFN YLKARRYVEA VDVCHKVIKA FPDYPKIRKE ILEKARMGLK P

UniProtKB: Intraflagellar transport protein 139

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分子 #18: IFT88

分子名称: IFT88 / タイプ: protein_or_peptide / ID: 18 / コピー数: 2 / 光学異性体: LEVO
由来(天然)生物種: Chlamydomonas reinhardtii (クラミドモナス)
分子量理論値: 86.418125 KDa
配列文字列: MSYGGTEEDD LYGGYDEQSN PLAGSGGAAF KALGADGAPP GTAMMGPPGT AMKSFVPGTA MRGGTAMQQD PSLARPMTSN RGAGFTSAP NKKFDPLNRS MGSTLGSSGG GAMLVARKGD TSPEEQARGM EKTVHELLEK SAADAAKNDI NSALENAMEA K KNERKLCR ...文字列:
MSYGGTEEDD LYGGYDEQSN PLAGSGGAAF KALGADGAPP GTAMMGPPGT AMKSFVPGTA MRGGTAMQQD PSLARPMTSN RGAGFTSAP NKKFDPLNRS MGSTLGSSGG GAMLVARKGD TSPEEQARGM EKTVHELLEK SAADAAKNDI NSALENAMEA K KNERKLCR FREQNNMADQ INLELMYAVD FNLAHMYHMN KNYSEALNLY TAIVRNKNFP QSGWLRVNMG NIHFEQKKYP SA IKMYRMA LDQISATAKE VRFKIMRNIG LSFVRMGQYP DALQSFATVM DNVPDHQTGY NLVMCNYALS DREGMKNAFI KLL KVSPPS EMDDDDDDDP MGDDDMQVMT MDDGLKDEMR KRNTIITRLI VKAAQLISEK VDRANGFEGG FMWCCEQLRD AGYT KLANE VELAKATRFM GQKQFDKAVG VFKDFEKKEP RVKARAATNL AFLYFLEGET DQADKYSEMA LKSDRYNARA YVNKG CVLV ERGDLEGARS LFNEAAGIDP YCVEAIYNLG LVSQRLNELP YALAAFKKLH NMVPDNVEVI HQIATTYDMM GDFKNA VKW FELLTSLVSN DPGVLARLGA IHARFDDEAK ALHYYQESHR VYPVNMDVIS WLGAYHVKSE VYEKAMPFFD LASKIQP QE VKWALMVASC YRRTNNLPAA LGKYKQIHTQ HPDNVECLRY LVHLCSELGR RAEAAEYMTK LKKAEKAAVP EATTAAAP A AAAAGSGMGG MGGLDDDIGS SAVSAQNRGK KMLVKEHMGG GGGKDNDDWG NEQLGDDLLP M

UniProtKB: Uncharacterized protein

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分子 #19: IFT70

分子名称: IFT70 / タイプ: protein_or_peptide / ID: 19 / コピー数: 2 / 光学異性体: LEVO
由来(天然)生物種: Chlamydomonas reinhardtii (クラミドモナス)
分子量理論値: 74.248609 KDa
配列文字列: MAFFQQPARP IAEGQYTQTI YTLIKEQKFA EAIQHLQYQL QNVPESRAAL SLLGYCYYYT GQYDMASQMY EQLVTLYPSN EDYKLYYAQ SLYKGGMYPE ASKAVVKVEG HQKAVTTLLV ACSYEQDDLT GCRRQLDKCA PEDPDTMVNT GCIMFKEGKF E AARQKFND ...文字列:
MAFFQQPARP IAEGQYTQTI YTLIKEQKFA EAIQHLQYQL QNVPESRAAL SLLGYCYYYT GQYDMASQMY EQLVTLYPSN EDYKLYYAQ SLYKGGMYPE ASKAVVKVEG HQKAVTTLLV ACSYEQDDLT GCRRQLDKCA PEDPDTMVNT GCIMFKEGKF E AARQKFND AVQALGYQPE LLYNIALCYY KTKQFGPALK HLAEIIEKAV REHPELSVGS NTDGMEVRSV GNSQTLKETA LI EAFNLKA AIEYTMKNVE AAKEALTDMP PRAEEELDPV TLHNSALINM DSDPTGGFKK LNFLLQSPPF PPETFANLLL LYC KPSHGF YDLAADVLAE NPQYAGKLLS PDLYDYLQAA IGRYKSPEEA FRRFDELATR HVEQLRRLTK QIQDARIARD NDAI KRAIN EYDEALEAYI PGLMAMASIY WDMELYSNVE KIFRQSAEFC SEHEVWKLNV AHTFFMQDNH YKEAIRYYEP VVKKN ADNL LGVTAIVLAN LCVSYIMTSQ NEEAEELMRK VEKEEERSSM QDPDKPCFHL CIINLVIGTL YCAKGNYEFG VSRIIK SLE PYDKKLETDT WYYAKRCFLA LIENLAKHMI VLKDSSFTEI MAFLNEAEKH GKDIRVVFNE GKHQSRTIAS EARMLKK MF LKLRD

UniProtKB: Uncharacterized protein

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分子 #20: Intraflagellar transport protein 57

分子名称: Intraflagellar transport protein 57 / タイプ: protein_or_peptide / ID: 20 / コピー数: 2 / 光学異性体: LEVO
由来(天然)生物種: Chlamydomonas reinhardtii (クラミドモナス)
分子量理論値: 51.374508 KDa
配列文字列: MSSKRGGRSS LAKAPEEAVN GEAFAPEASP PPPGDDGDAG GEDGGAPAPP PPPATKGGPV AVGRSLEIQT TPDVCMEMLA DKLKLLNYE ADFCRKKKPY RKPLSRLYFA VPLANSSEQF FYFTSLATWL LGLAGVELPA PKEFDDPNLT CQNILGAVKK L GFAPPSYH ...文字列:
MSSKRGGRSS LAKAPEEAVN GEAFAPEASP PPPGDDGDAG GEDGGAPAPP PPPATKGGPV AVGRSLEIQT TPDVCMEMLA DKLKLLNYE ADFCRKKKPY RKPLSRLYFA VPLANSSEQF FYFTSLATWL LGLAGVELPA PKEFDDPNLT CQNILGAVKK L GFAPPSYH PTKLTVGNGK EVVGVLDGLV DFVLERRHHK YSRPAYGNDG QPEEGVQLDD EAEAAAMEGA DELAMPAQNQ AD DDEEEEG VYVDPGRGDA AGPGTGASAA MDAEKAVLVS KVDPTLWKIE LERVAPKLRI TIAADSKDWR SHLDEAHQHK EVI SKAWPD SKTSLERLRA DLNGTLEKLQ TREKFLNEQF ESLMQQYRAA RTTFTDVQET YNRKTEAVAD RNQEMHRIGE TLEE VKAMM DEKGSNIADA TPVARIKTAI KQLNKELHDM EVRIGVVSHT LLQLSLRNKR LLQAQAALSD EEED

UniProtKB: Intraflagellar transport protein 57

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実験情報

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構造解析

手法クライオ電子顕微鏡法
解析サブトモグラム平均法
試料の集合状態cell

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試料調製

緩衝液pH: 7
凍結凍結剤: ETHANE

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電子顕微鏡法

顕微鏡TFS KRIOS
撮影フィルム・検出器のモデル: TFS FALCON 4i (4k x 4k)
平均電子線量: 2.6 e/Å2
電子線加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN
電子光学系照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス（公称値）: 4.0 µm / 最小 デフォーカス（公称値）: 2.0 µm
実験機器
モデル: Titan Krios / 画像提供: FEI Company

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画像解析

最終 再構成想定した対称性 - 点群: C2 (2回回転対称) / 解像度のタイプ: BY AUTHOR / 解像度: 15.4 Å / 解像度の算出法: FSC 0.143 CUT-OFF / ソフトウェア - 名称: RELION (ver. 3.1.3)
詳細: Composite map, with constituent maps ranging from 15.4 to 28.3 angstrom
使用したサブトモグラム数: 5896
抽出トモグラム数: 756 / 使用した粒子像数: 10455
CTF補正ソフトウェア - 名称: CTFFIND (ver. 4) / タイプ: PHASE FLIPPING AND AMPLITUDE CORRECTION
最終 角度割当タイプ: MAXIMUM LIKELIHOOD

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万見について

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お知らせ

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2022年2月9日: EMDBエントリの付随情報ファイルのフォーマットが新しくなりました

EMDBエントリの付随情報ファイルのフォーマットが新しくなりました

  • EMDBのヘッダファイルのバージョン3が、公式のフォーマットとなりました。
  • これまでは公式だったバージョン1.9は、アーカイブから削除されます。

関連情報:EMDBヘッダ

外部リンク:wwPDBはEMDBデータモデルのバージョン3へ移行します

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2020年8月12日: 新型コロナ情報

新型コロナ情報

URL: https://pdbj.org/emnavi/covid19.php

新ページ: EM Navigatorに新型コロナウイルスの特設ページを開設しました。

関連情報:Covid-19情報 / 2020年3月5日: 新型コロナウイルスの構造データ

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2020年3月5日: 新型コロナウイルスの構造データ

新型コロナウイルスの構造データ

関連情報:万見生物種 / 2020年8月12日: 新型コロナ情報

外部リンク:COVID-19特集ページ - PDBj / 今月の分子2020年2月：コロナウイルスプロテーアーゼ

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2019年1月31日: EMDBのIDの桁数の変更

EMDBのIDの桁数の変更

  • EMDBエントリに付与されているアクセスコード(EMDB-ID)は4桁の数字(例、EMD-1234)でしたが、間もなく枯渇します。これまでの4桁のID番号は4桁のまま変更されませんが、4桁の数字を使い切った後に発行されるIDは5桁以上の数字(例、EMD-12345)になります。5桁のIDは2019年の春頃から発行される見通しです。
  • EM Navigator/万見では、接頭語「EMD-」は省略されています。

関連情報:Q: 「EMD」とは何ですか？ / 万見/EM NavigatorにおけるID/アクセスコードの表記

外部リンク:EMDB Accession Codes are Changing Soon! / PDBjへお問い合わせ

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2017年7月12日: PDB大規模アップデート

PDB大規模アップデート

  • 新バージョンのPDBx/mmCIF辞書形式に基づくデータがリリースされました。
  • 今回の更新はバージョン番号が4から5になる大規模なもので、全エントリデータの書き換えが行われる「Remediation」というアップデートに該当します。
  • このバージョンアップで、電子顕微鏡の実験手法に関する多くの項目の書式が改定されました(例：em_softwareなど)。
  • EM NavigatorとYorodumiでも、この改定に基づいた表示内容になります。

外部リンク:wwPDB Remediation / OneDepデータ基準に準拠した、より強化された内容のモデル構造ファイルが、PDBアーカイブで公開されました。

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万見 (Yorodumi)

幾万の構造データを、幾万の視点から

  • 万見(Yorodumi)は、EMDB/PDB/SASBDBなどの構造データを閲覧するためのページです。
  • EM Navigatorの詳細ページの後継、Omokage検索のフロントエンドも兼ねています。

関連情報:EMDB / PDB / SASBDB / 3つのデータバンクの比較 / 万見検索 / 2016年8月31日: 新しいEM Navigatorと万見 / 万見文献 / Jmol/JSmol / 機能・相同性情報 / 新しいEM Navigatorと万見の変更点

他の情報も見る