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基本情報
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タイトル | Structure of dynein-2 intermediate chain DYNC2I1 (WDR60) in complex with the dynein-2 heavy chain DYNC2H1. | ||||||||||||||||||||||||||||||
![]() | Locally refined map, locally sharpened with locscale | ||||||||||||||||||||||||||||||
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![]() | dynein / cilia / intraflagellar transport / complex / TRANSPORT PROTEIN | ||||||||||||||||||||||||||||||
機能・相同性 | ![]() MGMT-mediated DNA damage reversal / intraciliary transport involved in cilium assembly / visual behavior / intraciliary retrograde transport / methylated-DNA-[protein]-cysteine S-methyltransferase / methylated-DNA-[protein]-cysteine S-methyltransferase activity / cilium movement involved in cell motility / 9+2 motile cilium / intraciliary transport / dynein light chain binding ...MGMT-mediated DNA damage reversal / intraciliary transport involved in cilium assembly / visual behavior / intraciliary retrograde transport / methylated-DNA-[protein]-cysteine S-methyltransferase / methylated-DNA-[protein]-cysteine S-methyltransferase activity / cilium movement involved in cell motility / 9+2 motile cilium / intraciliary transport / dynein light chain binding / ciliary transition zone / regulation of cilium assembly / spinal cord motor neuron differentiation / dynein heavy chain binding / DNA-methyltransferase activity / embryonic skeletal system morphogenesis / ciliary tip / Intraflagellar transport / dynein complex / DNA ligation / coronary vasculature development / minus-end-directed microtubule motor activity / non-motile cilium assembly / DNA alkylation repair / dynein light intermediate chain binding / protein localization to cilium / cytoplasmic dynein complex / positive regulation of smoothened signaling pathway / ciliary plasm / dorsal/ventral pattern formation / motile cilium / determination of left/right symmetry / embryonic limb morphogenesis / positive regulation of double-strand break repair / microtubule motor activity / ciliary base / dynein intermediate chain binding / pericentriolar material / microtubule-based movement / cytoskeletal motor activity / Golgi organization / axoneme / cilium assembly / Hedgehog 'off' state / forebrain development / centriole / ciliary basal body / filopodium / kidney development / methyltransferase activity / protein processing / cilium / apical part of cell / methylation / microtubule / DNA repair / centrosome / negative regulation of apoptotic process / Golgi apparatus / ATP hydrolysis activity / DNA binding / extracellular space / extracellular exosome / nucleoplasm / ATP binding / membrane / nucleus / metal ion binding / plasma membrane / cytoplasm / cytosol 類似検索 - 分子機能 | ||||||||||||||||||||||||||||||
生物種 | ![]() | ||||||||||||||||||||||||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.9 Å | ||||||||||||||||||||||||||||||
![]() | Mukhopadhyay AG / Toropova K / Daly L / Wells J / Vuolo L / Seda M / Jenkins D / Stephens DJ / Roberts AJ | ||||||||||||||||||||||||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Structure and tethering mechanism of dynein-2 intermediate chains in intraflagellar transport. 著者: Aakash G Mukhopadhyay / Katerina Toropova / Lydia Daly / Jennifer N Wells / Laura Vuolo / Miroslav Mladenov / Marian Seda / Dagan Jenkins / David J Stephens / Anthony J Roberts / ![]() 要旨: Dynein-2 is a large multiprotein complex that powers retrograde intraflagellar transport (IFT) of cargoes within cilia/flagella, but the molecular mechanism underlying this function is still emerging. ...Dynein-2 is a large multiprotein complex that powers retrograde intraflagellar transport (IFT) of cargoes within cilia/flagella, but the molecular mechanism underlying this function is still emerging. Distinctively, dynein-2 contains two identical force-generating heavy chains that interact with two different intermediate chains (WDR34 and WDR60). Here, we dissect regulation of dynein-2 function by WDR34 and WDR60 using an integrative approach including cryo-electron microscopy and CRISPR/Cas9-enabled cell biology. A 3.9 Å resolution structure shows how WDR34 and WDR60 use surprisingly different interactions to engage equivalent sites of the two heavy chains. We show that cilia can assemble in the absence of either WDR34 or WDR60 individually, but not both subunits. Dynein-2-dependent distribution of cargoes depends more strongly on WDR60, because the unique N-terminal extension of WDR60 facilitates dynein-2 targeting to cilia. Strikingly, this N-terminal extension can be transplanted onto WDR34 and retain function, suggesting it acts as a flexible tether to the IFT "trains" that assemble at the ciliary base. We discuss how use of unstructured tethers represents an emerging theme in IFT train interactions. | ||||||||||||||||||||||||||||||
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構造の表示
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ダウンロードとリンク
-EMDBアーカイブ
マップデータ | ![]() | 3.9 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 28.9 KB 28.9 KB | 表示 表示 | ![]() |
FSC (解像度算出) | ![]() | 19.2 KB | 表示 | ![]() |
画像 | ![]() | 101 KB | ||
マスクデータ | ![]() | 103 MB | ![]() | |
Filedesc metadata | ![]() | 10.3 KB | ||
その他 | ![]() ![]() ![]() | 95.6 MB 95.4 MB 95.4 MB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-検証レポート
文書・要旨 | ![]() | 1 MB | 表示 | ![]() |
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文書・詳細版 | ![]() | 1 MB | 表示 | |
XML形式データ | ![]() | 22 KB | 表示 | |
CIF形式データ | ![]() | 29.2 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||
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注釈 | Locally refined map, locally sharpened with locscale | ||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 0.828 Å | ||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
-マスク #1
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投影像・断面図 |
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密度ヒストグラム |
-追加マップ: Locally refined map, unsharpened
ファイル | emd_19133_additional_1.map | ||||||||||||
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注釈 | Locally refined map, unsharpened | ||||||||||||
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密度ヒストグラム |
-ハーフマップ: #2
ファイル | emd_19133_half_map_1.map | ||||||||||||
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密度ヒストグラム |
-ハーフマップ: #1
ファイル | emd_19133_half_map_2.map | ||||||||||||
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試料の構成要素
-全体 : Dynein-2 complex
全体 | 名称: Dynein-2 complex |
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要素 |
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-超分子 #1: Dynein-2 complex
超分子 | 名称: Dynein-2 complex / タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: all |
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由来(天然) | 生物種: ![]() |
-分子 #1: Methylated-DNA--protein-cysteine methyltransferase,Cytoplasmic dy...
分子 | 名称: Methylated-DNA--protein-cysteine methyltransferase,Cytoplasmic dynein 2 heavy chain 1 タイプ: protein_or_peptide / ID: 1 / 詳細: DYNC2H1 with N-terminal SNAPf tag / コピー数: 1 / 光学異性体: LEVO EC番号: methylated-DNA-[protein]-cysteine S-methyltransferase |
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由来(天然) | 生物種: ![]() |
分子量 | 理論値: 515.223031 KDa |
組換発現 | 生物種: ![]() ![]() |
配列 | 文字列: GDKDCEMKRT TLDSPLGKLE LSGCEQGLHR IIFLGKGTSA ADAVEVPAPA AVLGGPEPLM QATAWLNAYF HQPEAIEEFP VPALHHPVF QQESFTRQVL WKLLKVVKFG EVISYSHLAA LAGNPAATAA VKTALSGNPV PILIPCHRVV QGDLDVGGYE G GLAVKEWL ...文字列: GDKDCEMKRT TLDSPLGKLE LSGCEQGLHR IIFLGKGTSA ADAVEVPAPA AVLGGPEPLM QATAWLNAYF HQPEAIEEFP VPALHHPVF QQESFTRQVL WKLLKVVKFG EVISYSHLAA LAGNPAATAA VKTALSGNPV PILIPCHRVV QGDLDVGGYE G GLAVKEWL LAHEGHRLGK PGLGGSLEVL FQGPDYDIPT TLEVLFQGPA NGTADVRKLF IFTTTQNYFG LMSELWDQPL LC NCLEINN FLDDGNQMLL RVQRSDAGIS FSNTIEFGDT KDKVLVFFKL RPEVITDENL HDNILVSSML ESPISSLYQA VRQ VFAPML LKDQEWSRNF DPKLQNLLSE LEAGLGIVLR RSDTNLTKLK FKEDDTRGIL TPSDEFQFWI EQAHRGNKQI SKER ANYFK ELFETIAREF YNLDSLSLLE VVDLVETTQD VVDDVWRQTE HDHYPESRML HLLDIIGGSF GRFVQKKLGT LNLWE DPYY LVKESLKAGI SICEQWVIVC NHLTGQVWQR YVPHPWKNEK YFPETLDKLG KRLEEVLAIR TIHEKFLYFL PASEEK IIC LTRVFEPFTG LNPVQYNPYT EPLWKAAVSQ YEKIIAPAEQ KIAGKLKNYI SEIQDSPQQL LQAFLKYKEL VKRPTIS KE LMLERETLLA RLVDSIKDFR LDFENRCRGI PGDASGPLSG KNLSEVVNSI VWVRQLELKV DDTIKIAEAL LSDLPGFR C FHQSAKDLLD QLKLYEQEQF DDWSRDIQSG LSDSRSGLCI EASSRIMELD SNDGLLKVHY SDRLVILLRE VRQLSALGF VIPAKIQQVA NIAQKFCKQA IILKQVAHFY NSIDQQMIQS QRPMMLQSAL AFEQIIKNSK AGSGGKSQIT WDNPKELEGY IQKLQNAAE RLATENRKLR KWHTTFCEKV VVLMNIDLLR QQQRWKDGLQ ELRTGLATVE AQGFQASDMH AWKQHWNHQL Y KALEHQYQ MGLEALNENL PEINIDLTYK QGRLQFRPPF EEIRAKYYRE MKRFIGIPNQ FKGVGEAGDE SIFSIMIDRN AS GFLTIFS KAEDLFRRLS AVLHQHKEWI VIGQVDMEAL VEKHLFTVHD WEKNFKALKI KGKEVERLPS AVKVDCLNIN CNP VKTVID DLIQKLFDLL VLSLKKSIQA HLHEIDTFVT EAMEVLTIMP QSVEEIGDAN LQYSKLQERK PEILPLFQEA EDKN RLLRT VAGGGLETIS NLKAKWDKFE LMMESHQLMI KDQIEVMKGN VKSRLQIYYQ ELEKFKARWD QLKPGDDVIE TGQHN TLDK SAKLIKEKKI EFDDLEVTRK KLVDDCHHFR LEEPNFSLAS SISKDIESCA QIWAFYEEFQ QGFQEMANED WITFRT KTY LFEEFLMNWH DRLRKVEEHS VMTVKLQSEV DKYKIVIPIL KYVRGEHLSP DHWLDLFRLL GLPRGTSLEK LLFGDLL RV ADTIVAKAAD LKDLNSRAQG EVTIREALRE LDLWGVGAVF TLIDYEDSQS RTMKLIKDWK DIVNQVGDNR CLLQSLKD S PYYKGFEDKV SIWERKLAEL DEYLQNLNHI QRKWVYLEPI FGRGALPKEQ TRFNRVDEDF RSIMTDIKKD NRVTTLTTH AGIRNSLLTI LDQLQRCQRS LNEFLEEKRS AFPRFYFIGD DDLLEILGQS TNPSVIQSHL KKLFAGINSV CFDEKSKHIT AMKSLEGEV VPFKNKVPLS NNVETWLNDL ALEMKKTLEQ LLKECVTTGR SSQGAVDPSL FPSQILCLAE QIKFTEDVEN A IKDHSLHQ IETQLVNKLE QYTNIDTSSE DPGNTESGIL ELKLKALILD IIHNIDVVKQ LNQIQVHTTE DWAWKKQLRF YM KSDHTCC VQMVDSEFQY TYEYQGNASK LVYTPLTDKC YLTLTQAMKM GLGGNPYGPA GTGKTESVKA LGGLLGRQVL VFN CDEGID VKSMGRIFVG LVKCGAWGCF DEFNRLEESV LSAVSMQIQT IQDALKNHRT VCELLGKEVE VNSNSGIFIT MNPA GKGYG GRQKLPDNLK QLFRPVAMSH PDNELIAEVI LYSEGFKDAK VLSRKLVAIF NLSRELLTPQ QHYDWGLRAL KTVLR GSGN LLRQLNKSGT TQNANESHIV VQALRLNTMS KFTFTDCTRF DALIKDVFPG IELKEVEYDE LSAALKQVFE EANYEI IPN QIKKALELYE QLCQRMGVVI VGPSGAGKST LWRMLRAALC KTGKVVKQYT MNPKAMPRYQ LLGHIDMDTR EWSDGVL TN SARQVVREPQ DVSSWIICDG DIDPEWIESL NSVLDDNRLL TMPSGERIQF GPNVNFVFET HDLSCASPAT ISRMGMIF L SDEETDLNSL IKSWLRNQPA EYRNNLENWI GDYFEKALQW VLKQNDYVVE TSLVGTVMNG LSHLHGCRDH DEFIINLIR GLGGNLNMKS RLEFTKEVFH WARESPPDFH KPMDTYYDST RGRLATYVLK KPEDLTADDF SNGLTLPVIQ TPDMQRGLDY FKPWLSSDT KQPFILVGPE GCGKGMLLRY AFSQLRSTQI ATVHCSAQTT SRHLLQKLSQ TCMVISTNTG RVYRPKDCER L VLYLKDIN LPKLDKWGTS TLVAFLQQVL TYQGFYDENL EWVGLENIQI VASMSAGGRL GRHKLTTRFT SIVRLCSIDY PE REQLQTI YGAYLEPVLH KNLKNHSIWG SSSKIYLLAG SMVQVYEQVR AKFTVDDYSH YFFTPCILTQ WVLGLFRYDL EGG SSNHPL DYVLEIVAYE ARRLFRDKIV GAKELHLFDI ILTSVFQGDW GSDILDNMSD SFYVTWGARH NSGARAAPGQ PLPP HGKPL GKLNSTDLKD VIKKGLIHYG RDNQNLDILL FHEVLEYMSR IDRVLSFPGG SLLLAGRSGV GRRTITSLVS HMHGA VLFS PKISRGYELK QFKNDLKHVL QLAGIEAQQV VLLLEDYQFV HPTFLEMINS LLSSGEVPGL YTLEELEPLL LPLKDQ ASQ DGFFGPVFNY FTYRIQQNLH IVLIMDSANS NFMINCESNP ALHKKCQVLW MEGWSNSSMK KIPEMLFSET GGGEKYN DK KRKEEKKKNS VDPDFLKSFL LIHESCKAYG ATPSQYMTFL HVYSAISSSK KKELLKRQSH LQAGVSKLNE AKALVDEL N RKAGEQSVLL KTKQDEADAA LQMITVSMQD ASEQKTELER LKHRIAEEVV KIEERKNKID DELKEVQPLV NEAKLAVGN IKPESLSEIR SLRMPPDVIR DILEGVLRLM GIFDTSWVSM KSFLAKRGVR EDIATFDARN ISKEIRESVE ELLFKNKGSF DPKNAKRAS TAAAPLAAWV KANIQYSHVL ERIHPLETEQ AGLESNLKKT EDRKRKLEEL LNSVGQKVSE LKEKFQSRTS E AAKLEAEV SKAQETIKAA EVLINQLDRE HKRWNAQVVE ITEELATLPK RAQLAAAFIT YLSAAPESLR KTCLEEWTKS AG LEKFDLR RFLCTESEQL IWKSEGLPSD DLSIENALVI LQSRVCPFLI DPSSQATEWL KTHLKDSRLE VINQQDSNFI TAL ELAVRF GKTLIIQEMD GVEPVLYPLL RRDLVAQGPR YVVQIGDKII DYNEEFRLFL STRNPNPFIP PDAASIVTEV NFTT TRSGL RGQLLALTIQ HEKPDLEEQK TKLLQQEEDK KIQLAKLEES LLETLATSQG NILENKDLIE SLNQTKASSA LIQES LKES YKLQISLDQE RDAYLPLAES ASKMYFIISD LSKINNMYRF SLAAFLRLFQ RALQNKQDSE NTEQRIQSLI SSLQHM VYE YICRCLFKAD QLMFALHFVR GMHPELFQEN EWDTFTGVVV GDMLRKADSQ QKIRDQLPSW IDQERSWAVA TLKIALP SL YQTLCFEDAA LWRTYYNNSM CEQEFPSILA KKVSLFQQIL VVQVLRPDRL QSAMALFACK TLGLKEVSPL PLNLKRLY K ETLEIEPILI IISPGADPSQ ELQELANAER SGECYHQVAM GQGQADLAIQ MLKECARNGD WLCLKNLHLV VSWLPVLEK ELNTLQPKDT FRLWLTAEVH PNFTPILLQS SLKITYESPP GLKKNLMRTY ESWTPEQISK KDNTHRAHAL FSLAWFHAAC QERRNYIPQ GWTKFYEFSL SDLRAGYNII DRLFDGAKDV QWEFVHGLLE NAIYGGRIDN YFDLRVLQSY LKQFFNSSVI D VFNQRNKK SIFPYSVSLP QSCSILDYRA VIEKIPEDDK PSFFGLPANI ARSSQRMISS QVISQLRILG RSITAGSKFD RE IWSNELS PVLNLWKKLN QNSNLIHQKV PPPNDRQGSP ILSFIILEQF NAIRLVQSVH QSLAALSKVI RGTTLLSSEV QKL ASALLN QKCPLAWQSK WEGPEDPLQY LRGLVARALA IQNWVDKAEK QALLSETLDL SELFHPDTFL NALRQETARA VGRS VDSLK FVASWKGRLQ EAKLQIKISG LLLEGCSFDG NQLSENQLDS PSVSSVLPCF MGWIPQDACG PYSPDECISL PVYTS AERD RVVTNIDVPC GGNQDQWIQC GAALFLKNQ UniProtKB: Methylated-DNA--protein-cysteine methyltransferase, Cytoplasmic dynein 2 heavy chain 1 |
-分子 #2: Cytoplasmic dynein 2 intermediate chain 1
分子 | 名称: Cytoplasmic dynein 2 intermediate chain 1 / タイプ: protein_or_peptide / ID: 2 / コピー数: 1 / 光学異性体: LEVO |
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由来(天然) | 生物種: ![]() |
分子量 | 理論値: 122.865156 KDa |
組換発現 | 生物種: ![]() ![]() |
配列 | 文字列: MEPGKRRTKD DTWKADDLRK HLWAIQSGGS KEERKHREKK LRKESEMDLP EHKEPRCRDP DQDARSRDRV AEVHTAKESP RGERDRDRQ RERRRDAKDR EKEKLKEKHR EAEKSHSRGK DREKEKDRRA RKEELRQTVA HHNLLGQETR DRQLLERAER K GRSVSKVR ...文字列: MEPGKRRTKD DTWKADDLRK HLWAIQSGGS KEERKHREKK LRKESEMDLP EHKEPRCRDP DQDARSRDRV AEVHTAKESP RGERDRDRQ RERRRDAKDR EKEKLKEKHR EAEKSHSRGK DREKEKDRRA RKEELRQTVA HHNLLGQETR DRQLLERAER K GRSVSKVR SEEKDEDSER GDEDRERRYR ERKLQYGDSK DNPLKYWLYK EEGERRHRKP REPDRDKKHR EKSSTREKRE KY SKEKSNS FSDKGEERHK EKRHKEGFHF DDERHQSNVD RKEKSAKDEP RKREFQNGEH RNRGASSKRD GTSSQHAENL VRN HGKDKD SRRKHGHEEG SSVWWKLDQR PGGEETVEIE KEETDLENAR ADAYTASCED DFEDYEDDFE VCDGDDDESS NEPE SREKL EELPLAQKKE IQEIQRAINA ENERIGELSL KLFQKRGRTE FEKEPRTDTN SSPSRASVCG IFVDFASASH RQKSR TQAL KQKMRSTKLL RLIDLDFSFT FSLLDLPPVN EYDMYIRNFG KKNTKQAYVQ CNEDNVERDI QTEEIETREV WTQHPG EST VVSGGSEQRD TSDAVVMPKI DTPRLCSFLR AACQVMAVLL EEDRLAAEPS WNLRAQDRAL YFSDSSSQLN TSLPFLQ NR KVSSLHTSRV QRQMVVSVHD LPEKSFVPLL DSKYVLCVWD IWQPSGPQKV LICESQVTCC CLSPLKAFLL FAGTAHGS V VVWDLREDSR LHYSVTLSDG FWTFRTATFS TDGILTSVNH RSPLQAVEPI STSVHKKQSF VLSPFSTQEE MSGLSFHIA SLDESGVLNV WVVVELPKAD IAGSISDLGL MPGGRVKLVH SALIQLGDSL SHKGNEFWGT TQTLNVKFLP SDPNHFIIGT DMGLISHGT RQDLRVAPKL FKPQQHGIRP VKVNVIDFSP FGEPIFLAGC SDGSIRLHQL SSAFPLLQWD SSTDSHAVTG L QWSPTRPA VFLVQDDTSN IYIWDLLQSD LGPVAKQQVS PNRLVAMAAV GEPEKAGGSF LALVLARASG SIDIQHLKRR WA APEVDEC NRLRLLLQEA LWPEGKLHK UniProtKB: Cytoplasmic dynein 2 intermediate chain 1 |
-分子 #3: Cytoplasmic dynein 2 intermediate chain 2
分子 | 名称: Cytoplasmic dynein 2 intermediate chain 2 / タイプ: protein_or_peptide / ID: 3 詳細: DYNC2I2 (also known as WDR34) with C-terminal Strep tag コピー数: 1 / 光学異性体: LEVO |
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由来(天然) | 生物種: ![]() |
分子量 | 理論値: 60.639129 KDa |
組換発現 | 生物種: ![]() ![]() |
配列 | 文字列: MATRAQPGPL SQAGSAGVAA LATVGVASGP GPGRPGPLQD ETLGVASVPS QWRAVQGIRG ETKSCQTASI ATASASAQAR NHVDAQVQT EAPVPVSVQP PSQYDIPRLA AFLRRVEAMV IRELNKNWQS HAFDGFEVNW TEQQQMVSCL YTLGYPPAQA Q GLHVTSIS ...文字列: MATRAQPGPL SQAGSAGVAA LATVGVASGP GPGRPGPLQD ETLGVASVPS QWRAVQGIRG ETKSCQTASI ATASASAQAR NHVDAQVQT EAPVPVSVQP PSQYDIPRLA AFLRRVEAMV IRELNKNWQS HAFDGFEVNW TEQQQMVSCL YTLGYPPAQA Q GLHVTSIS WNSTGSVVAC AYGRLDHGDW STLKSFVCAW NLDRRDLRPQ QPSAVVEVPS AVLCLAFHPT QPSHVAGGLY SG EVLVWDL SRLEDPLLWR TGLTDDTHTD PVSQVVWLPE PGHSHRFQVL SVATDGKVLL WQGIGVGQLQ LTEGFALVMQ QLP RSTKLK KHPRGETEVG ATAVAFSSFD PRLFILGTEG GFPLKCSLAA GEAALTRMPS SVPLRAPAQF TFSPHGGPIY SVSC SPFHR NLFLSAGTDG HVHLYSMLQA PPLTSLQLSL KYLFAVRWSP VRPLVFAAAS GKGDVQLFDL QKSSQKPTVL IKQTQ DESP VYCLEFNSQQ TQLLAAGDAQ GTVKVWQLST EFTEQGPREA EDLDCLAAEV AAWSHPQFEK GSAGSAAGSG AGWSHP QFE K UniProtKB: Cytoplasmic dynein 2 intermediate chain 2 |
-分子 #4: Cytoplasmic dynein 2 light intermediate chain 1
分子 | 名称: Cytoplasmic dynein 2 light intermediate chain 1 / タイプ: protein_or_peptide / ID: 4 / コピー数: 1 / 光学異性体: LEVO |
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由来(天然) | 生物種: ![]() |
分子量 | 理論値: 39.681621 KDa |
組換発現 | 生物種: ![]() ![]() |
配列 | 文字列: MPSETLWEIA KAEVEKRGIN GSEGDGAEIA EKFVFFIGSK NGGKTTIILR CLDRDEPPKP TLALEYTYGR RAKGHNTPKD IAHFWELGG GTSLLDLISI PITGDTLRTF SLVLVLDLSK PNDLWPTMEN LLQATKSHVD KVIMKLGKTN AKAVSEMRQK I WNNMPKDH ...文字列: MPSETLWEIA KAEVEKRGIN GSEGDGAEIA EKFVFFIGSK NGGKTTIILR CLDRDEPPKP TLALEYTYGR RAKGHNTPKD IAHFWELGG GTSLLDLISI PITGDTLRTF SLVLVLDLSK PNDLWPTMEN LLQATKSHVD KVIMKLGKTN AKAVSEMRQK I WNNMPKDH PDHELIDPFP VPLVIIGSKY DVFQDFESEK RKVICKTLRF VAHYYGASLM FTSKSEALLL KIRGVINQLA FG IDKSKSI CVDQNKPLFI TAGLDSFGQI GSPPVPENDI GKLHAHSPME LWKKVYEKLF PPKSINTLKD IKDPARDPQY AEN EVDEMR IQKDLELEQY KRSSSKSWKQ IELDS UniProtKB: Cytoplasmic dynein 2 light intermediate chain 1 |
-分子 #5: Dynein light chain roadblock-type 1
分子 | 名称: Dynein light chain roadblock-type 1 / タイプ: protein_or_peptide / ID: 5 / コピー数: 2 / 光学異性体: LEVO |
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由来(天然) | 生物種: ![]() |
分子量 | 理論値: 10.934576 KDa |
組換発現 | 生物種: ![]() ![]() |
配列 | 文字列: MAEVEETLKR LQSQKGVQGI IVVNTEGIPI KSTMDNPTTT QYASLMHSFI LKARSTVRDI DPQNDLTFLR IRSKKNEIMV APDKDYFLI VIQNPTE UniProtKB: Dynein light chain roadblock-type 1 |
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
緩衝液 | pH: 7.5 |
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凍結 | 凍結剤: ETHANE |
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電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: GATAN K3 BIOQUANTUM (6k x 4k) 平均電子線量: 50.6 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 3.5 µm / 最小 デフォーカス(公称値): 1.5 µm |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |