EMDB-14008: Structure of recombinant human gamma-Tubulin Ring Complex 8-spoke...

基本情報

• 登録情報: データベース: EMDB / ID: EMD-14008
• タイトル: Structure of recombinant human gamma-Tubulin Ring Complex 8-spoked assembly intermediate (spokes 7-14)
• マップデータ: 8-spoked assembly intermediate of the gamma-Tubulin Ring Complex (spokes 7-14)

試料

• 複合体: Recombinant human Gamma-Tubulin Ring Complex
  • タンパク質・ペプチド: Tubulin gamma-1 chain
  • タンパク質・ペプチド: Mitotic-spindle organizing protein 1
  • タンパク質・ペプチド: Gamma-tubulin complex component 3
  • タンパク質・ペプチド: Gamma-tubulin complex component 5
  • タンパク質・ペプチド: Gamma-tubulin complex component 2
  • タンパク質・ペプチド: Gamma-tubulin complex component 4
  • タンパク質・ペプチド: Gamma-tubulin complex component 6

機能・相同性

分子機能:

microtubule nucleation by interphase microtubule organizing center / gamma-tubulin complex localization / equatorial microtubule organizing center / mitotic spindle microtubule / gamma-tubulin ring complex / microtubule minus-end binding / interphase microtubule organizing center / polar microtubule / gamma-tubulin complex / meiotic spindle organization / microtubule nucleation / gamma-tubulin binding / non-motile cilium / microtubule organizing center / pericentriolar material / cell leading edge / cytoplasmic microtubule / mitotic sister chromatid segregation / mitotic spindle assembly / single fertilization / spindle assembly / cytoplasmic microtubule organization / Loss of Nlp from mitotic centrosomes / Loss of proteins required for interphase microtubule organization from the centrosome / Recruitment of mitotic centrosome proteins and complexes / Recruitment of NuMA to mitotic centrosomes / Anchoring of the basal body to the plasma membrane / centriole / AURKA Activation by TPX2 / mitotic spindle organization / ciliary basal body / meiotic cell cycle / condensed nuclear chromosome / neuron migration / brain development / recycling endosome / structural constituent of cytoskeleton / spindle pole / microtubule cytoskeleton organization / spindle / microtubule cytoskeleton / Regulation of PLK1 Activity at G2/M Transition / apical part of cell / mitotic cell cycle / microtubule binding / protein-containing complex assembly / microtubule / neuron projection / centrosome / GTP binding / structural molecule activity / nucleoplasm / identical protein binding / membrane / nucleus / cytoplasm / cytosol

ドメイン・相同性:

Mitotic-spindle organizing protein 1 / Mitotic-spindle organizing gamma-tubulin ring associated / Gamma-tubulin complex component 6, N-terminal / Gamma-tubulin complex component 6 N-terminus / Gamma tubulin / Gamma tubulin complex component, C-terminal / Gamma-tubulin complex component, C-terminal domain superfamily / Gamma tubulin complex component C-terminal / Gamma-tubulin complex component protein / Gamma tubulin complex component protein, N-terminal / Gamma tubulin complex component N-terminal / Tubulin / Tubulin, C-terminal / Tubulin C-terminal domain / Tubulin, conserved site / Tubulin subunits alpha, beta, and gamma signature. / Tubulin/FtsZ family, C-terminal domain / Tubulin/FtsZ-like, C-terminal domain / Tubulin/FtsZ, C-terminal / Tubulin/FtsZ, 2-layer sandwich domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ, GTPase domain / Tubulin/FtsZ, GTPase domain superfamily

構成要素:

Tubulin gamma-1 chain / Mitotic-spindle organizing protein 1 / Gamma-tubulin complex component 3 / Gamma-tubulin complex component 6 / Gamma-tubulin complex component 5 / Gamma-tubulin complex component 2 / Gamma-tubulin complex component 4

• 生物種: Homo sapiens (ヒト)
• 手法: 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 7.6 Å
• データ登録者: Zupa E / Pfeffer S

資金援助

ドイツ, 2件

Organization	Grant number	国
German Research Foundation (DFG)	PF 963/1-4	ドイツ
German Research Foundation (DFG)	Schi 295/4-4	ドイツ

• 引用: ジャーナル: Nat Commun / 年: 2022; タイトル: Modular assembly of the principal microtubule nucleator γ-TuRC.; 著者: Martin Würtz / Erik Zupa / Enrico S Atorino / Annett Neuner / Anna Böhler / Ariani S Rahadian / Bram J A Vermeulen / Giulia Tonon / Sebastian Eustermann / Elmar Schiebel / Stefan Pfeffer / ; 要旨: The gamma-tubulin ring complex (γ-TuRC) is the principal microtubule nucleation template in vertebrates. Recent cryo-EM reconstructions visualized the intricate quaternary structure of the γ-TuRC, containing more than thirty subunits, raising fundamental questions about γ-TuRC assembly and the role of actin as an integral part of the complex. Here, we reveal the structural mechanism underlying modular γ-TuRC assembly and identify a functional role of actin in microtubule nucleation. During γ-TuRC assembly, a GCP6-stabilized core comprising GCP2-3-4-5-4-6 is expanded by stepwise recruitment, selective stabilization and conformational locking of four pre-formed GCP2-GCP3 units. Formation of the lumenal bridge specifies incorporation of the terminal GCP2-GCP3 unit and thereby leads to closure of the γ-TuRC ring in a left-handed spiral configuration. Actin incorporation into the complex is not relevant for γ-TuRC assembly and structural integrity, but determines γ-TuRC geometry and is required for efficient microtubule nucleation and mitotic chromosome alignment in vivo.

履歴

登録	2021年12月16日	-
ヘッダ(付随情報) 公開	2022年1月26日	-
マップ公開	2022年1月26日	-
更新	2022年2月9日	-
現状	2022年2月9日	処理サイト: PDBe / 状態: 公開