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- EMDB-13767: IMC-Arches-Stalk C1 at 6.18A - Refinement of the IMC, Arches and ... -

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Entry
Database: EMDB / ID: EMD-13767
TitleIMC-Arches-Stalk C1 at 6.18A - Refinement of the IMC, Arches and Stalk complex without symmetry from the fully-assembled R388 type IV secretion system.
Map dataIMC-Arches-Stalk C1 6.18A sharpened - Refinement of the Inner Membrane Complex (IMC), Arches and Stalk without symmetry.
Sample
  • Complex: Type IV secretion system complex
    • Protein or peptide: TrwG protein
Function / homologyType IV secretion system protein VirB8/PtlE / Bacterial virulence protein VirB8 / VirB8 protein / protein secretion by the type IV secretion system / NTF2-like domain superfamily / membrane / TrwG protein
Function and homology information
Biological speciesEscherichia coli (E. coli)
Methodsingle particle reconstruction / cryo EM / Resolution: 6.18 Å
AuthorsMace K / Vadakkepat AK / Lukoyanova N / Waksman G
Funding support United Kingdom, 4 items
OrganizationGrant numberCountry
Wellcome Trust098302 United Kingdom
Wellcome Trust217089 United Kingdom
Wellcome Trust202679/Z/16/Z United Kingdom
Wellcome Trust206166/Z/17/Z United Kingdom
CitationJournal: Nature / Year: 2022
Title: Cryo-EM structure of a type IV secretion system.
Authors: Kévin Macé / Abhinav K Vadakkepat / Adam Redzej / Natalya Lukoyanova / Clasien Oomen / Nathalie Braun / Marta Ukleja / Fang Lu / Tiago R D Costa / Elena V Orlova / David Baker / Qian Cong ...Authors: Kévin Macé / Abhinav K Vadakkepat / Adam Redzej / Natalya Lukoyanova / Clasien Oomen / Nathalie Braun / Marta Ukleja / Fang Lu / Tiago R D Costa / Elena V Orlova / David Baker / Qian Cong / Gabriel Waksman /
Abstract: Bacterial conjugation is the fundamental process of unidirectional transfer of DNAs, often plasmid DNAs, from a donor cell to a recipient cell. It is the primary means by which antibiotic resistance ...Bacterial conjugation is the fundamental process of unidirectional transfer of DNAs, often plasmid DNAs, from a donor cell to a recipient cell. It is the primary means by which antibiotic resistance genes spread among bacterial populations. In Gram-negative bacteria, conjugation is mediated by a large transport apparatus-the conjugative type IV secretion system (T4SS)-produced by the donor cell and embedded in both its outer and inner membranes. The T4SS also elaborates a long extracellular filament-the conjugative pilus-that is essential for DNA transfer. Here we present a high-resolution cryo-electron microscopy (cryo-EM) structure of a 2.8 megadalton T4SS complex composed of 92 polypeptides representing 8 of the 10 essential T4SS components involved in pilus biogenesis. We added the two remaining components to the structural model using co-evolution analysis of protein interfaces, to enable the reconstitution of the entire system including the pilus. This structure describes the exceptionally large protein-protein interaction network required to assemble the many components that constitute a T4SS and provides insights on the unique mechanism by which they elaborate pili.
History
DepositionOct 21, 2021-
Header (metadata) releaseJun 22, 2022-
Map releaseJun 22, 2022-
UpdateJul 20, 2022-
Current statusJul 20, 2022Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_13767.png

Downloads & links

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Map

FileDownload / File: emd_13767.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationIMC-Arches-Stalk C1 6.18A sharpened - Refinement of the Inner Membrane Complex (IMC), Arches and Stalk without symmetry.
Voxel sizeX=Y=Z: 1.067 Å
Density
Contour LevelBy AUTHOR: 0.05
Minimum - Maximum-0.19870856 - 0.38187966
Average (Standard dev.)0.002284417 (±0.0104121715)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions512512512
Spacing512512512
CellA=B=C: 546.304 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_13767_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Additional map: IMC-Arches-Stalk C1 6.18A unsharpened - Refinement of the...

Fileemd_13767_additional_1.map
AnnotationIMC-Arches-Stalk C1 6.18A unsharpened - Refinement of the Inner Membrane Complex (IMC), Arches and Stalk without symmetry.
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: IMC-Arches-Stalk C1 6.18A - Half-A

Fileemd_13767_half_map_1.map
AnnotationIMC-Arches-Stalk C1 6.18A - Half-A
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: IMC-Arches-Stalk C1 6.18A - Half-B

Fileemd_13767_half_map_2.map
AnnotationIMC-Arches-Stalk C1 6.18A - Half-B
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Type IV secretion system complex

EntireName: Type IV secretion system complex
Components
  • Complex: Type IV secretion system complex
    • Protein or peptide: TrwG protein

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Supramolecule #1: Type IV secretion system complex

SupramoleculeName: Type IV secretion system complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Escherichia coli (E. coli) / Strain: R388 plasmid
Recombinant expressionOrganism: Escherichia coli (E. coli)
Recombinant plasmid: pBADM11_trwN/virB1-trwE/virB10Strep_rbstrwD/virB11_rbsHistrwB /virD4
Molecular weightTheoretical: 2.808 MDa

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Macromolecule #1: TrwG protein

MacromoleculeName: TrwG protein / type: protein_or_peptide / ID: 1 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 25.799994 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MSKKQPKPVK AEQLKSYYEE SRGLERDLIG EFVKSRKTAW RVATASGLFG LLGMVCGIVG FSQPAPAPLV LRVDNATGAV DVVTTLREH ESSYGEVVDT YWLNQYVLNR EAYDYNTIQM NYDTTALLSA PAVQQDYYKL FDGSNARDRV LGNKARITVR V RSIQPNGR ...String:
MSKKQPKPVK AEQLKSYYEE SRGLERDLIG EFVKSRKTAW RVATASGLFG LLGMVCGIVG FSQPAPAPLV LRVDNATGAV DVVTTLREH ESSYGEVVDT YWLNQYVLNR EAYDYNTIQM NYDTTALLSA PAVQQDYYKL FDGSNARDRV LGNKARITVR V RSIQPNGR GQATVRFTTQ QHNSNGTVEA PQHQIATIGY TYIGAPMRSS DRLLNPLGFQ VTSYRADPEI LNN

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.6
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 57.5 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: OTHER / Imaging mode: OTHER
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: OTHER / Details: CryoSPARC ab-initio
Final reconstructionResolution.type: BY AUTHOR / Resolution: 6.18 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 566815
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: OTHER / Details: Stochastic gradient descent (SGD)

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