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- EMDB-12351: Human ATM kinase with bound inhibitor KU-55933 (composite structure) -

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Basic information

Entry
Database: EMDB / ID: EMD-12351
TitleHuman ATM kinase with bound inhibitor KU-55933 (composite structure)
Map dataHuman ATM full length with bound inhibitor KU-55933 composite map LAFTER filtered
Sample
  • Complex: ATM dimer with bound KU-55933
    • Protein or peptide: Serine-protein kinase ATM
  • Ligand: 2-morpholin-4-yl-6-thianthren-1-yl-pyran-4-one
  • Ligand: ZINC ION
KeywordsKinase / inhibitor / DNA damage response / cancer research / SIGNALING PROTEIN
Function / homology
Function and homology information


positive regulation of DNA catabolic process / establishment of RNA localization to telomere / positive regulation of telomerase catalytic core complex assembly / positive regulation of DNA damage response, signal transduction by p53 class mediator / establishment of protein-containing complex localization to telomere / negative regulation of telomere capping / Sensing of DNA Double Strand Breaks / cellular response to nitrosative stress / regulation of microglial cell activation / meiotic telomere clustering ...positive regulation of DNA catabolic process / establishment of RNA localization to telomere / positive regulation of telomerase catalytic core complex assembly / positive regulation of DNA damage response, signal transduction by p53 class mediator / establishment of protein-containing complex localization to telomere / negative regulation of telomere capping / Sensing of DNA Double Strand Breaks / cellular response to nitrosative stress / regulation of microglial cell activation / meiotic telomere clustering / positive regulation of telomere maintenance via telomere lengthening / pre-B cell allelic exclusion / male meiotic nuclear division / histone mRNA catabolic process / female meiotic nuclear division / extrinsic component of synaptic vesicle membrane / regulation of telomere maintenance via telomerase / cellular response to X-ray / peptidyl-serine autophosphorylation / DNA double-strand break processing / pexophagy / lipoprotein catabolic process / regulation of autophagosome assembly / V(D)J recombination / Impaired BRCA2 binding to PALB2 / oocyte development / reciprocal meiotic recombination / DNA repair complex / Homologous DNA Pairing and Strand Exchange / Defective homologous recombination repair (HRR) due to BRCA1 loss of function / Defective HDR through Homologous Recombination Repair (HRR) due to PALB2 loss of BRCA1 binding function / Defective HDR through Homologous Recombination Repair (HRR) due to PALB2 loss of BRCA2/RAD51/RAD51C binding function / Resolution of D-loop Structures through Synthesis-Dependent Strand Annealing (SDSA) / Resolution of D-loop Structures through Holliday Junction Intermediates / HDR through Single Strand Annealing (SSA) / 1-phosphatidylinositol-3-kinase activity / cellular response to stress / Impaired BRCA2 binding to RAD51 / response to ionizing radiation / TP53 Regulates Transcription of Caspase Activators and Caspases / mitotic spindle assembly checkpoint signaling / negative regulation of B cell proliferation / mitotic G2 DNA damage checkpoint signaling / TP53 Regulates Transcription of Genes Involved in Cytochrome C Release / Presynaptic phase of homologous DNA pairing and strand exchange / peroxisomal matrix / replicative senescence / signal transduction in response to DNA damage / Regulation of HSF1-mediated heat shock response / somitogenesis / : / regulation of cellular response to heat / ovarian follicle development / cellular response to retinoic acid / negative regulation of TORC1 signaling / positive regulation of telomere maintenance via telomerase / telomere maintenance / positive regulation of cell adhesion / Pexophagy / post-embryonic development / DNA damage checkpoint signaling / thymus development / regulation of signal transduction by p53 class mediator / regulation of autophagy / cellular response to reactive oxygen species / determination of adult lifespan / TP53 Regulates Transcription of DNA Repair Genes / Nonhomologous End-Joining (NHEJ) / Stabilization of p53 / Autodegradation of the E3 ubiquitin ligase COP1 / double-strand break repair via homologous recombination / cellular response to gamma radiation / G2/M DNA damage checkpoint / brain development / multicellular organism growth / DNA Damage/Telomere Stress Induced Senescence / HDR through Homologous Recombination (HRR) / Regulation of TP53 Activity through Methylation / Meiotic recombination / double-strand break repair via nonhomologous end joining / spindle / intrinsic apoptotic signaling pathway in response to DNA damage / cellular senescence / positive regulation of neuron apoptotic process / Regulation of TP53 Degradation / double-strand break repair / Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks / site of double-strand break / heart development / Processing of DNA double-strand break ends / peptidyl-serine phosphorylation / protein autophosphorylation / regulation of apoptotic process / neuron apoptotic process / Regulation of TP53 Activity through Phosphorylation / eukaryotic translation initiation factor 2alpha kinase activity / 3-phosphoinositide-dependent protein kinase activity / DNA-dependent protein kinase activity / ribosomal protein S6 kinase activity / histone H3S10 kinase activity
Similarity search - Function
Telomere-length maintenance and DNA damage repair / Serine/threonine-protein kinase ATM, plant / ATM, catalytic domain / Telomere-length maintenance and DNA damage repair / Telomere-length maintenance and DNA damage repair / FATC domain / PIK-related kinase, FAT / FAT domain / FATC / FATC domain ...Telomere-length maintenance and DNA damage repair / Serine/threonine-protein kinase ATM, plant / ATM, catalytic domain / Telomere-length maintenance and DNA damage repair / Telomere-length maintenance and DNA damage repair / FATC domain / PIK-related kinase, FAT / FAT domain / FATC / FATC domain / PIK-related kinase / FAT domain profile. / FATC domain profile. / Phosphatidylinositol 3- and 4-kinases signature 1. / Phosphatidylinositol 3/4-kinase, conserved site / Phosphatidylinositol 3- and 4-kinases signature 2. / Phosphatidylinositol 3-/4-kinase, catalytic domain superfamily / Phosphoinositide 3-kinase, catalytic domain / Phosphatidylinositol 3- and 4-kinase / Phosphatidylinositol 3- and 4-kinases catalytic domain profile. / Phosphatidylinositol 3-/4-kinase, catalytic domain / Armadillo-type fold / Protein kinase-like domain superfamily
Similarity search - Domain/homology
Serine-protein kinase ATM
Similarity search - Component
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.78 Å
AuthorsBartho JD / Stakyte K / Rotheneder M / Lammens K / Hopfner KP
Funding support Germany, 4 items
OrganizationGrant numberCountry
German Research Foundation (DFG)CRC1054 Germany
German Research Foundation (DFG)CRC1064 Germany
German Research Foundation (DFG)CRC1361 Germany
Leibniz Association Germany
CitationJournal: Nat Struct Mol Biol / Year: 2021
Title: Molecular basis of human ATM kinase inhibition.
Authors: K Stakyte / M Rotheneder / K Lammens / J D Bartho / U Grädler / T Fuchß / U Pehl / A Alt / E van de Logt / K P Hopfner /
Abstract: Human checkpoint kinase ataxia telangiectasia-mutated (ATM) plays a key role in initiation of the DNA damage response following DNA double-strand breaks. ATM inhibition is a promising approach in ...Human checkpoint kinase ataxia telangiectasia-mutated (ATM) plays a key role in initiation of the DNA damage response following DNA double-strand breaks. ATM inhibition is a promising approach in cancer therapy, but, so far, detailed insights into the binding modes of known ATM inhibitors have been hampered due to the lack of high-resolution ATM structures. Using cryo-EM, we have determined the structure of human ATM to an overall resolution sufficient to build a near-complete atomic model and identify two hitherto unknown zinc-binding motifs. We determined the structure of the kinase domain bound to ATPγS and to the ATM inhibitors KU-55933 and M4076 at 2.8 Å, 2.8 Å and 3.0 Å resolution, respectively. The mode of action and selectivity of the ATM inhibitors can be explained by structural comparison and provide a framework for structure-based drug design.
History
DepositionFeb 11, 2021-
Header (metadata) releaseSep 1, 2021-
Map releaseSep 1, 2021-
UpdateJul 10, 2024-
Current statusJul 10, 2024Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 5.8
  • Imaged by UCSF Chimera
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  • Surface view colored by cylindrical radius
  • Surface level: 5.8
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-7ni5
  • Surface level: 5.8
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_12351.png

Downloads & links

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Map

FileDownload / File: emd_12351.map.gz / Format: CCP4 / Size: 1000 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationHuman ATM full length with bound inhibitor KU-55933 composite map LAFTER filtered
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesX (Sec.)Y (Row.)Z (Col.)
0.53 Å/pix.
x 640 pix.
= 338.88 Å		0.53 Å/pix.
x 640 pix.
= 338.88 Å		0.53 Å/pix.
x 640 pix.
= 338.88 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.5295 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 5.8 / Movie #1: 5.8
Minimum - Maximum-30.206600000000002 - 50.162019999999998
Average (Standard dev.)0.002536232 (±1.2428328)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderZYX
Origin000
Dimensions640640640
Spacing640640640
CellA=B=C: 338.88 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z0.52950.52950.5295
M x/y/z640640640
origin x/y/z0.0000.0000.000
length x/y/z338.880338.880338.880
α/β/γ90.00090.00090.000
start NX/NY/NZ000
NX/NY/NZ640640640
MAP C/R/S321
start NC/NR/NS000
NC/NR/NS640640640
D min/max/mean-30.20750.1620.003

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Supplemental data

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Sample components

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Entire : ATM dimer with bound KU-55933

EntireName: ATM dimer with bound KU-55933
Components
  • Complex: ATM dimer with bound KU-55933
    • Protein or peptide: Serine-protein kinase ATM
  • Ligand: 2-morpholin-4-yl-6-thianthren-1-yl-pyran-4-one
  • Ligand: ZINC ION

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Supramolecule #1: ATM dimer with bound KU-55933

SupramoleculeName: ATM dimer with bound KU-55933 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1
Source (natural)Organism: Homo sapiens (human)

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Macromolecule #1: Serine-protein kinase ATM

MacromoleculeName: Serine-protein kinase ATM / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO / EC number: non-specific serine/threonine protein kinase
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 354.526188 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MDYKDDDDKG TDYKDDDDKS GSLEVLFQGP MSLVLNDLLI CCRQLEHDRA TERKKEVEKF KRLIRDPETI KHLDRHSDSK QGKYLNWDA VFRFLQKYIQ KETECLRIAK PNVSASTQAS RQKKMQEISS LVKYFIKCAN RRAPRLKCQE LLNYIMDTVK D SSNGAIYG ...String:
MDYKDDDDKG TDYKDDDDKS GSLEVLFQGP MSLVLNDLLI CCRQLEHDRA TERKKEVEKF KRLIRDPETI KHLDRHSDSK QGKYLNWDA VFRFLQKYIQ KETECLRIAK PNVSASTQAS RQKKMQEISS LVKYFIKCAN RRAPRLKCQE LLNYIMDTVK D SSNGAIYG ADCSNILLKD ILSVRKYWCE ISQQQWLELF SVYFRLYLKP SQDVHRVLVA RIIHAVTKGC CSQTDGLNSK FL DFFSKAI QCARQEKSSS GLNHILAALT IFLKTLAVNF RIRVCELGDE ILPTLLYIWT QHRLNDSLKE VIIELFQLQI YIH HPKGAK TQEKGAYEST KWRSILYNLY DLLVNEISHI GSRGKYSSGF RNIAVKENLI ELMADICHQV FNEDTRSLEI SQSY TTTQR ESSDYSVPCK RKKIELGWEV IKDHLQKSQN DFDLVPWLQI ATQLISKYPA SLPNCELSPL LMILSQLLPQ QRHGE RTPY VLRCLTEVAL CQDKRSNLES SQKSDLLKLW NKIWCITFRG ISSEQIQAEN FGLLGAIIQG SLVEVDREFW KLFTGS ACR PSCPAVCCLT LALTTSIVPG TVKMGIEQNM CEVNRSFSLK ESIMKWLLFY QLEGDLENST EVPPILHSNF PHLVLEK IL VSLTMKNCKA AMNFFQSVPE CEHHQKDKEE LSFSEVEELF LQTTFDKMDF LTIVRECGIE KHQSSIGFSV HQNLKESL D RCLLGLSEQL LNNYSSEITN SETLVRCSRL LVGVLGCYCY MGVIAEEEAY KSELFQKAKS LMQCAGESIT LFKNKTNEE FRIGSLRNMM QLCTRCLSNC TKKSPNKIAS GFFLRLLTSK LMNDIADICK SLASFIKKPF DRGEVESMED DTNGNLMEVE DQSSMNLFN DYPDSSVSDA NEPGESQSTI GAINPLAEEY LSKQDLLFLD MLKFLCLCVT TAQTNTVSFR AADIRRKLLM L IDSSTLEP TKSLHLHMYL MLLKELPGEE YPLPMEDVLE LLKPLSNVCS LYRRDQDVCK TILNHVLHVV KNLGQSNMDS EN TRDAQGQ FLTVIGAFWH LTKERKYIFS VRMALVNCLK TLLEADPYSK WAILNVMGKD FPVNEVFTQF LADNHHQVRM LAA ESINRL FQDTKGDSSR LLKALPLKLQ QTAFENAYLK AQEGMREMSH SAENPETLDE IYNRKSVLLT LIAVVLSCSP ICEK QALFA LCKSVKENGL EPHLVKKVLE KVSETFGYRR LEDFMASHLD YLVLEWLNLQ DTEYNLSSFP FILLNYTNIE DFYRS CYKV LIPHLVIRSH FDEVKSIANQ IQEDWKSLLT DCFPKILVNI LPYFAYEGTR DSGMAQQRET ATKVYDMLKS ENLLGK QID HLFISNLPEI VVELLMTLHE PANSSASQST DLCDFSGDLD PAPNPPHFPS HVIKATFAYI SNCHKTKLKS ILEILSK SP DSYQKILLAI CEQAAETNNV YKKHRILKIY HLFVSLLLKD IKSGLGGAWA FVLRDVIYTL IHYINQRPSC IMDVSLRS F SLCCDLLSQV CQTAVTYCKD ALENHLHVIV GTLIPLVYEQ VEVQKQVLDL LKYLVIDNKD NENLYITIKL LDPFPDHVV FKDLRITQQK IKYSRGPFSL LEEINHFLSV SVYDALPLTR LEGLKDLRRQ LELHKDQMVD IMRASQDNPQ DGIMVKLVVN LLQLSKMAI NHTGEKEVLE AVGSCLGEVG PIDFSTIAIQ HSKDASYTKA LKLFEDKELQ WTFIMLTYLN NTLVEDCVKV R SAAVTCLK NILATKTGHS FWEIYKMTTD PMLAYLQPFR TSRKKFLEVP RFDKENPFEG LDDINLWIPL SENHDIWIKT LT CAFLDSG GTKCEILQLL KPMCEVKTDF CQTVLPYLIH DILLQDTNES WRNLLSTHVQ GFFTSCLRHF SQTSRSTTPA NLD SESEHF FRCCLDKKSQ RTMLAVVDYM RRQKRPSSGT IFNDAFWLDL NYLEVAKVAQ SCAAHFTALL YAEIYADKKS MDDQ EKRSL AFEEGSQSTT ISSLSEKSKE ETGISLQDLL LEIYRSIGEP DSLYGCGGGK MLQPITRLRT YEHEAMWGKA LVTYD LETA IPSSTRQAGI IQALQNLGLC HILSVYLKGL DYENKDWCPE LEELHYQAAW RNMQWDHCTS VSKEVEGTSY HESLYN ALQ SLRDREFSTF YESLKYARVK EVEEMCKRSL ESVYSLYPTL SRLQAIGELE SIGELFSRSV THRQLSEVYI KWQKHSQ LL KDSDFSFQEP IMALRTVILE ILMEKEMDNS QRECIKDILT KHLVELSILA RTFKNTQLPE RAIFQIKQYN SVSCGVSE W QLEEAQVFWA KKEQSLALSI LKQMIKKLDA SCAANNPSLK LTYTECLRVC GNWLAETCLE NPAVIMQTYL EKAVEVAGN YDGESSDELR NGKMKAFLSL ARFSDTQYQR IENYMKSSEF ENKQALLKRA KEEVGLLREH KIQTNRYTVK VQRELELDEL ALRALKEDR KRFLCKAVEN YINCLLSGEE HDMWVFRLCS LWLENSGVSE VNGMMKRDGM KIPTYKFLPL MYQLAARMGT K MMGGLGFH EVLNNLISRI SMDHPHHTLF IILALANANR DEFLTKPEVA RRSRITKNVP KQSSQLDEDR TEAANRIICT IR SRRPQMV RSVEALCDAY IILANLDATQ WKTQRKGINI PADQPITKLK NLEDVVVPTM EIKVDHTGEY GNLVTIQSFK AEF RLAGGV NLPKIIDCVG SDGKERRQLV KGRDDLRQDA VMQQVFQMCN TLLQRNTETR KRKLTICTYK VVPLSQRSGV LEWC TGTVP IGEFLVNNED GAHKRYRPND FSAFQCQKKM MEVQKKSFEE KYEVFMDVCQ NFQPVFRYFC MEKFLDPAIW FEKRL AYTR SVATSSIVGY ILGLGDRHVQ NILINEQSAE LVHIDLGVAF EQGKILPTPE TVPFRLTRDI VDGMGITGVE GVFRRC CEK TMEVMRNSQE TLLTIVEVLL YDPLFDWTMN PLKALYLQQR PEDETELHPT LNADDQECKR NLSDIDQSFN KVAERVL MR LQEKLKGVEE GTVLSVGGQV NLLIQQAIDP KNLSRLFPGW KAWV

UniProtKB: Serine-protein kinase ATM

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Macromolecule #2: 2-morpholin-4-yl-6-thianthren-1-yl-pyran-4-one

MacromoleculeName: 2-morpholin-4-yl-6-thianthren-1-yl-pyran-4-one / type: ligand / ID: 2 / Number of copies: 2 / Formula: UF8
Molecular weightTheoretical: 395.495 Da
Chemical component information

ChemComp-UF8:
2-morpholin-4-yl-6-thianthren-1-yl-pyran-4-one

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Macromolecule #3: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 3 / Number of copies: 4 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Average electron dose: 42.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.78 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 1039118
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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