OpuA (E190Q) %u2013 Glycine betaine, ATP at 3.3A resolution
試料
複合体: OpuA (E190Q) occluded
タンパク質・ペプチド: ABC-type proline/glycine betaine transport system ATPase component
タンパク質・ペプチド: ABC transporter permease subunit
リガンド: ADENOSINE-5'-TRIPHOSPHATE
リガンド: TRIMETHYL GLYCINE
キーワード
osmoregulation / ABC-transporter / glycine betaine uptake system / MEMBRANE PROTEIN
機能・相同性
機能・相同性情報
amine transmembrane transporter activity / ABC-type quaternary amine transporter / ABC-type quaternary ammonium compound transporting activity / carnitine transmembrane transporter activity / glycine betaine transport / choline transport / peptide transport / amino acid transport / ATP-binding cassette (ABC) transporter complex / protein transport ...amine transmembrane transporter activity / ABC-type quaternary amine transporter / ABC-type quaternary ammonium compound transporting activity / carnitine transmembrane transporter activity / glycine betaine transport / choline transport / peptide transport / amino acid transport / ATP-binding cassette (ABC) transporter complex / protein transport / ATP hydrolysis activity / ATP binding / membrane 類似検索 - 分子機能
Glycine betaine transport ATP-binding subunit / ABC-type glycine betaine transport system, substrate-binding domain / Substrate binding domain of ABC-type glycine betaine transport system / ABC transporter type 1, transmembrane domain MetI-like / MetI-like superfamily / Binding-protein-dependent transport system inner membrane component / ABC transporter integral membrane type-1 domain profile. / CBS domain superfamily / CBS domain / CBS domain ...Glycine betaine transport ATP-binding subunit / ABC-type glycine betaine transport system, substrate-binding domain / Substrate binding domain of ABC-type glycine betaine transport system / ABC transporter type 1, transmembrane domain MetI-like / MetI-like superfamily / Binding-protein-dependent transport system inner membrane component / ABC transporter integral membrane type-1 domain profile. / CBS domain superfamily / CBS domain / CBS domain / CBS domain profile. / ABC transporter-like, conserved site / ABC transporters family signature. / ABC transporter / ABC transporter-like, ATP-binding domain / ATP-binding cassette, ABC transporter-type domain profile. / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / P-loop containing nucleoside triphosphate hydrolase 類似検索 - ドメイン・相同性
Quaternary amine transport ATP-binding protein / ABC transporter permease/substrate binding protein / Glycine betaine transport ATP-binding protein OpuAA 類似検索 - 構成要素
Netherlands Organisation for Scientific Research (NWO)
722.017.001
オランダ
Netherlands Organisation for Scientific Research (NWO)
740.018.016
オランダ
引用
ジャーナル: Sci Adv / 年: 2020 タイトル: Gating by ionic strength and safety check by cyclic-di-AMP in the ABC transporter OpuA. 著者: Hendrik R Sikkema / Marco van den Noort / Jan Rheinberger / Marijn de Boer / Sabrina T Krepel / Gea K Schuurman-Wolters / Cristina Paulino / Bert Poolman / 要旨: (Micro)organisms are exposed to fluctuating environmental conditions, and adaptation to stress is essential for survival. Increased osmolality (hypertonicity) causes outflow of water and loss of ...(Micro)organisms are exposed to fluctuating environmental conditions, and adaptation to stress is essential for survival. Increased osmolality (hypertonicity) causes outflow of water and loss of turgor and is dangerous if the cell is not capable of rapidly restoring its volume. The osmoregulatory adenosine triphosphate-binding cassette transporter OpuA restores the cell volume by accumulating large amounts of compatible solute. OpuA is gated by ionic strength and inhibited by the second messenger cyclic-di-AMP, a molecule recently shown to affect many cellular processes. Despite the master regulatory role of cyclic-di-AMP, structural and functional insights into how the second messenger regulates (transport) proteins on the molecular level are lacking. Here, we present high-resolution cryo-electron microscopy structures of OpuA and in vitro activity assays that show how the osmoregulator OpuA is activated by high ionic strength and how cyclic-di-AMP acts as a backstop to prevent unbridled uptake of compatible solutes.