EMDB-11602: Structure of a human ABCE1-bound 43S pre-initiation complex - Sta...

Basic information

• Entry: Database: EMDB / ID: EMD-11602
• Title: Structure of a human ABCE1-bound 43S pre-initiation complex - State III

Sample

• Complex: ABCE1-bound 43S pre-initiation complex
  • Protein or peptide: x 52 types
  • RNA: x 2 types
• Ligand: x 7 types

Function / homology

Function:

negative regulation of endoribonuclease activity / male germ cell proliferation / CTPase activity / positive regulation of mRNA binding / regulation of translation in response to endoplasmic reticulum stress / translation initiation ternary complex / glial limiting end-foot / response to kainic acid / viral translational termination-reinitiation / HRI-mediated signaling / Cellular response to mitochondrial stress / positive regulation of mRNA cis splicing, via spliceosome / response to manganese-induced endoplasmic reticulum stress / eukaryotic translation initiation factor 3 complex, eIF3e / positive regulation of type B pancreatic cell apoptotic process / methionyl-initiator methionine tRNA binding / cap-dependent translational initiation / eukaryotic translation initiation factor 3 complex, eIF3m / negative regulation of translational initiation in response to stress / Response of EIF2AK1 (HRI) to heme deficiency / Recycling of eIF2:GDP / translation reinitiation / PERK-mediated unfolded protein response / selenocysteine metabolic process / IRES-dependent viral translational initiation / PERK regulates gene expression / eukaryotic translation initiation factor 2 complex / regulation of translational initiation in response to stress / multi-eIF complex / eukaryotic translation initiation factor 3 complex / formation of cytoplasmic translation initiation complex / OAS antiviral response / selenocysteine incorporation / eukaryotic 43S preinitiation complex / selenocysteine insertion sequence binding / protein-synthesizing GTPase / cytoplasmic translational initiation / translation factor activity, RNA binding / mRNA cap binding / formation of translation preinitiation complex / positive regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis / negative regulation of endoplasmic reticulum unfolded protein response / oxidized pyrimidine DNA binding / eukaryotic 48S preinitiation complex / response to TNF agonist / positive regulation of base-excision repair / protein tyrosine kinase inhibitor activity / positive regulation of respiratory burst involved in inflammatory response / regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway / positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage / positive regulation of gastrulation / nucleolus organization / IRE1-RACK1-PP2A complex / : / positive regulation of endodeoxyribonuclease activity / positive regulation of Golgi to plasma membrane protein transport / TNFR1-mediated ceramide production / negative regulation of RNA splicing / negative regulation of DNA repair / ribosome disassembly / metal-dependent deubiquitinase activity / negative regulation of intrinsic apoptotic signaling pathway in response to hydrogen peroxide / oxidized purine DNA binding / supercoiled DNA binding / neural crest cell differentiation / NF-kappaB complex / ubiquitin-like protein conjugating enzyme binding / regulation of translational initiation / regulation of establishment of cell polarity / negative regulation of phagocytosis / positive regulation of ubiquitin-protein transferase activity / rRNA modification in the nucleus and cytosol / Formation of the ternary complex, and subsequently, the 43S complex / erythrocyte homeostasis / cytoplasmic side of rough endoplasmic reticulum membrane / laminin receptor activity / nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / protein kinase A binding / negative regulation of ubiquitin protein ligase activity / Ribosomal scanning and start codon recognition / ion channel inhibitor activity / Translation initiation complex formation / pigmentation / positive regulation of mitochondrial depolarization / mammalian oogenesis stage / activation-induced cell death of T cells / positive regulation of T cell receptor signaling pathway / negative regulation of Wnt signaling pathway / fibroblast growth factor binding / positive regulation of activated T cell proliferation / iron-sulfur cluster binding / regulation of cell division / Protein hydroxylation / negative regulation of peptidyl-serine phosphorylation / BH3 domain binding / mTORC1-mediated signalling / SARS-CoV-1 modulates host translation machinery / Peptide chain elongation / monocyte chemotaxis / positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator

Domain/homology:

Eukaryotic translation initiation factor 3 subunit D / Eukaryotic translation initiation factor 3 subunit 7 (eIF-3) / Eukaryotic translation initiation factor 3 subunit H / eIF3h, C-terminal / C-terminal region of eIF3h / Eukaryotic translation initiation factor 3 subunit F / Translation initiation factor 3 complex subunit L / RNA polymerase I-associated factor PAF67 / Eukaryotic translation initiation factor 3 subunit M / eIF3 subunit M, C-terminal helix domain / eIF3 subunit M, C-terminal helix / Eukaryotic translation initiation factor 3 subunit E, C-terminal / Eukaryotic translation initiation factor 3 subunit E / Eukaryotic translation initiation factor 3 subunit E, N-terminal / eIF3 subunit 6 N terminal domain / eIF3 subunit 6 N terminal domain / Eukaryotic translation initiation factor 3 subunit K / Translation initiation factor 3, subunit 12, N-terminal, eukaryotic / Eukaryotic translation initiation factor 3 subunit I / Eukaryotic translation initiation factor SUI1 / RLI, domain 1 / RLI1 / RNase L inhibitor RLI-like, possible metal-binding domain / Eukaryotic translation initiation factor 3 subunit B / eIF3B, RNA recognition motif / Possible Fer4-like domain in RNase L inhibitor, RLI / SUI1 domain superfamily / Eukaryotic translation initiation factor 3 subunit M eIF3m/COP9 signalosome complex subunit 7 COPS7 / Translation initiation factor SUI1 / Translation initiation factor SUI1 family profile. / Eukaryotic translation initiation factor 3 subunit C, N-terminal domain / Eukaryotic translation initiation factor 3 subunit C / Eukaryotic translation initiation factor 3 subunit 8 N-terminus / Translation initiation factor, beta propellor-like domain / Eukaryotic translation initiation factor 3 subunit A / SUI1 domain / : / Eukaryotic translation initiation factor eIF2A / Translation initiation factor IF2/IF5 / Translation initiation factor IF2/IF5 domain / Translation initiation factor IF2/IF5, N-terminal / Translation initiation factor IF2/IF5, zinc-binding / Domain found in IF2B/IF5 / domain present in translation initiation factor eIF2B and eIF5 / Translation initiation factor 1A (eIF-1A), conserved site / Eukaryotic initiation factor 1A signature. / eukaryotic translation initiation factor 1A / Translation initiation factor 1A (eIF-1A) / IF2a, S1-like domain / Translation initiation factor 2, alpha subunit / Translation initiation factor 2, alpha subunit, middle domain superfamily / Translation initiation factor 2, alpha subunit, C-terminal / Eukaryotic translation initiation factor 2 alpha subunit / Initiation factor eIF2 gamma, domain 2 / Initiation factor eIF2 gamma, GTP-binding domain / Initiation factor eIF2 gamma, C-terminal / Initiation factor eIF2 gamma, C terminal / RNA-binding domain, S1, IF1 type / Translation initiation factor 1A / IF-1 / S1 domain IF1 type profile. / 4Fe-4S binding domain / Anaphase-promoting complex subunit 4, WD40 domain / CSN8/PSMD8/EIF3K / CSN8/PSMD8/EIF3K family / Anaphase-promoting complex subunit 4 WD40 domain / Rpn11/EIF3F, C-terminal / Maintenance of mitochondrial structure and function / motif in proteasome subunits, Int-6, Nip-1 and TRIP-15 / PCI domain / Proteasome component (PCI) domain / PCI domain profile. / 40S ribosomal protein SA / 40S ribosomal protein SA, C-terminal domain / 40S ribosomal protein SA C-terminus / Ubiquitin-like protein FUBI / Translation elongation factor EF1A/initiation factor IF2gamma, C-terminal / : / JAB1/Mov34/MPN/PAD-1 ubiquitin protease / : / Ribosomal protein S26e signature. / S1 domain profile. / : / Ribosomal protein L41 / Ribosomal protein L41 / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S26e / Ribosomal protein S21e, conserved site / Ribosomal protein S21e signature. / Ribosomal protein S12e signature. / Ribosomal protein S12e / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S19e, conserved site / Ribosomal protein S19e signature. / Small (40S) ribosomal subunit Asc1/RACK1 / Ribosomal protein S2, eukaryotic / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / S27a-like superfamily

Component:

40S ribosomal protein S24 / Eukaryotic translation initiation factor 3 subunit F / Eukaryotic translation initiation factor 3 subunit D / Eukaryotic translation initiation factor 3 subunit H / Eukaryotic translation initiation factor 2 subunit 1 / Small ribosomal subunit protein eS17 / Small ribosomal subunit protein uS2 / Small ribosomal subunit protein uS5 / Eukaryotic translation initiation factor 2 subunit 2 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein eS12 / Small ribosomal subunit protein eS19 / Eukaryotic translation initiation factor 2 subunit 3 / Eukaryotic translation initiation factor 1 / Small ribosomal subunit protein eS27 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein eS10 / Eukaryotic translation initiation factor 1A, X-chromosomal / Eukaryotic translation initiation factor 3 subunit B / Eukaryotic translation initiation factor 3 subunit E / Small ribosomal subunit protein uS10 / ATP-binding cassette sub-family E member 1 / Small ribosomal subunit protein eS1 / Small ribosomal subunit protein eS7 / Small ribosomal subunit protein eS8 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS9 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein uS17 / Small ribosomal subunit protein eS4, X isoform / Small ribosomal subunit protein eS6 / Small ribosomal subunit protein uS19 / Small ribosomal subunit protein eS25 / Small ribosomal subunit protein eS26 / Small ribosomal subunit protein eS28 / Ubiquitin-like FUBI-ribosomal protein eS30 fusion protein / Small ribosomal subunit protein eS32 / Ubiquitin-ribosomal protein eS31 fusion protein / Small ribosomal subunit protein eS21 / Small ribosomal subunit protein RACK1 / Eukaryotic translation initiation factor 3 subunit I / Eukaryotic translation initiation factor 3 subunit A / Eukaryotic translation initiation factor 3 subunit M / Eukaryotic translation initiation factor 3 subunit C / Eukaryotic translation initiation factor 3 subunit K / Eukaryotic translation initiation factor 3 subunit L

• Biological species: Homo sapiens (human) / Human (human)
• Method: single particle reconstruction / cryo EM / Resolution: 3.5 Å
• Authors: Kratzat H / Mackens-Kiani T / Ameismeier A / Cheng J / Berninghausen O / Becker T / Beckmann R

Funding support

Germany, France, 2 items

Organization	Grant number	Country
German Research Foundation (DFG)		Germany
French National Research Agency		France

• Citation: Journal: EMBO J / Year: 2021; Title: A structural inventory of native ribosomal ABCE1-43S pre-initiation complexes.; Authors: Hanna Kratzat / Timur Mackens-Kiani / Michael Ameismeier / Mia Potocnjak / Jingdong Cheng / Estelle Dacheux / Abdelkader Namane / Otto Berninghausen / Franz Herzog / Micheline Fromont-Racine / Thomas Becker / Roland Beckmann / ; Abstract: In eukaryotic translation, termination and ribosome recycling phases are linked to subsequent initiation of a new round of translation by persistence of several factors at ribosomal sub-complexes. These comprise/include the large eIF3 complex, eIF3j (Hcr1 in yeast) and the ATP-binding cassette protein ABCE1 (Rli1 in yeast). The ATPase is mainly active as a recycling factor, but it can remain bound to the dissociated 40S subunit until formation of the next 43S pre-initiation complexes. However, its functional role and native architectural context remains largely enigmatic. Here, we present an architectural inventory of native yeast and human ABCE1-containing pre-initiation complexes by cryo-EM. We found that ABCE1 was mostly associated with early 43S, but also with later 48S phases of initiation. It adopted a novel hybrid conformation of its nucleotide-binding domains, while interacting with the N-terminus of eIF3j. Further, eIF3j occupied the mRNA entry channel via its ultimate C-terminus providing a structural explanation for its antagonistic role with respect to mRNA binding. Overall, the native human samples provide a near-complete molecular picture of the architecture and sophisticated interaction network of the 43S-bound eIF3 complex and the eIF2 ternary complex containing the initiator tRNA.

History

Deposition	Aug 7, 2020	-
Header (metadata) release	Oct 14, 2020	-
Map release	Oct 14, 2020	-
Update	Jan 13, 2021	-
Current status	Jan 13, 2021	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_11602.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 1.059 Å

Density

Contour Level	By AUTHOR: 0.01 / Movie #1: 0.014
Minimum - Maximum	-0.030029478 - 0.086862944
Average (Standard dev.)	-3.4457528e-06 (±0.0038568315)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 400 400 400 Spacing 400 400 400
Cell	A=B=C: 423.6 Å α=β=γ: 90.0 °

CCP4 map header:

mode	Image stored as Reals
Å/pix. X/Y/Z	1.059	1.059	1.059
M x/y/z	400	400	400
origin x/y/z	0.000	0.000	0.000
length x/y/z	423.600	423.600	423.600
α/β/γ	90.000	90.000	90.000
MAP C/R/S	1	2	3
start NC/NR/NS	0	0	0
NC/NR/NS	400	400	400
D min/max/mean	-0.030	0.087	-0.000

Supplemental data

Additional map: local resolution filtered map

• File: emd_11602_additional_1.map
• Annotation: local resolution filtered map

Additional map: focused refinement on ABCE1, post-processed map

• File: emd_11602_additional_10.map
• Annotation: focused refinement on ABCE1, post-processed map

Additional map: multi body refinement body 1

• File: emd_11602_additional_2.map
• Annotation: multi body refinement body 1

Additional map: multi body refinement body 2

• File: emd_11602_additional_3.map
• Annotation: multi body refinement body 2

Additional map: multi body refinement body 3

• File: emd_11602_additional_4.map
• Annotation: multi body refinement body 3

Additional map: multi body refinement body 4

• File: emd_11602_additional_5.map
• Annotation: multi body refinement body 4

Additional map: post-processed and masked map

• File: emd_11602_additional_6.map
• Annotation: post-processed and masked map

Additional map: focused refinement on ABCE1, local resolution filtered map

• File: emd_11602_additional_7.map
• Annotation: focused refinement on ABCE1, local resolution filtered map

Additional map: focused refinement on TC, local resolution filtered map

• File: emd_11602_additional_8.map
• Annotation: focused refinement on TC, local resolution filtered map

Additional map: focused refinement on TC, local resolution filtered map

• File: emd_11602_additional_9.map
• Annotation: focused refinement on TC, local resolution filtered map

Sample components

Entire : ABCE1-bound 43S pre-initiation complex

• Entire: Name: ABCE1-bound 43S pre-initiation complex

Components

• Complex: ABCE1-bound 43S pre-initiation complex
  • Protein or peptide: 40S ribosomal protein SA
  • Protein or peptide: 40S ribosomal protein S3a
  • Protein or peptide: 40S ribosomal protein S2
  • Protein or peptide: 40S ribosomal protein S26
  • Protein or peptide: 40S ribosomal protein S4, X isoform
  • Protein or peptide: 60S ribosomal protein L41
  • Protein or peptide: 40S ribosomal protein S6
  • Protein or peptide: 40S ribosomal protein S7
  • Protein or peptide: 40S ribosomal protein S8
  • Protein or peptide: 40S ribosomal protein S9
  • Protein or peptide: 40S ribosomal protein S11
  • Protein or peptide: 40S ribosomal protein S13
  • Protein or peptide: 40S ribosomal protein S21
  • Protein or peptide: 40S ribosomal protein S15a
  • Protein or peptide: 40S ribosomal protein S24
  • Protein or peptide: 40S ribosomal protein S27
  • Protein or peptide: 40S ribosomal protein S30
  • RNA: 18S ribosomal RNA
  • Protein or peptide: 40S ribosomal protein S17
  • Protein or peptide: 40S ribosomal protein S3
  • Protein or peptide: 40S ribosomal protein S5
  • Protein or peptide: 40S ribosomal protein S10
  • Protein or peptide: 40S ribosomal protein S12
  • Protein or peptide: 40S ribosomal protein S15
  • Protein or peptide: 40S ribosomal protein S16
  • Protein or peptide: 40S ribosomal protein S18
  • Protein or peptide: 40S ribosomal protein S19
  • Protein or peptide: 40S ribosomal protein S20
  • Protein or peptide: 40S ribosomal protein S25
  • Protein or peptide: 40S ribosomal protein S28
  • Protein or peptide: 40S ribosomal protein S29
  • Protein or peptide: Ubiquitin-40S ribosomal protein S27a
  • Protein or peptide: Receptor of activated protein C kinase 1
  • Protein or peptide: 40S ribosomal protein S14
  • Protein or peptide: 40S ribosomal protein S23
  • Protein or peptide: Eukaryotic translation initiation factor 1A, X-chromosomal
  • Protein or peptide: Eukaryotic translation initiation factor 3 subunit I
  • Protein or peptide: Eukaryotic translation initiation factor 3 subunit B
  • Protein or peptide: Eukaryotic translation initiation factor 3 subunit A
  • Protein or peptide: Eukaryotic translation initiation factor 3 subunit C,Eukaryotic translation initiation factor 3 subunit C,Eukaryotic translation initiation factor 3 subunit C
  • Protein or peptide: Eukaryotic translation initiation factor 3 subunit E
  • Protein or peptide: Eukaryotic translation initiation factor 3 subunit F
  • Protein or peptide: Eukaryotic translation initiation factor 3 subunit H
  • Protein or peptide: Eukaryotic translation initiation factor 3 subunit K
  • Protein or peptide: Eukaryotic translation initiation factor 3 subunit L
  • Protein or peptide: Eukaryotic translation initiation factor 3 subunit M
  • Protein or peptide: ATP-binding cassette sub-family E member 1
  • Protein or peptide: Eukaryotic translation initiation factor 3 subunit D
  • Protein or peptide: RNA recognition motif
  • RNA: tRNA
  • Protein or peptide: Eukaryotic translation initiation factor 2 subunit 2
  • Protein or peptide: Eukaryotic translation initiation factor 2 subunit 1
  • Protein or peptide: Eukaryotic translation initiation factor 2 subunit 3
  • Protein or peptide: Eukaryotic translation initiation factor 1
• Ligand: ZINC ION
• Ligand: IRON/SULFUR CLUSTER
• Ligand: ADENOSINE-5'-DIPHOSPHATE
• Ligand: MAGNESIUM ION
• Ligand: ADENOSINE-5'-TRIPHOSPHATE
• Ligand: METHIONINE
• Ligand: GUANOSINE-5'-TRIPHOSPHATE

Supramolecule #1: ABCE1-bound 43S pre-initiation complex

• Supramolecule: Name: ABCE1-bound 43S pre-initiation complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#54
• Source (natural): Organism: Homo sapiens (human)

Macromolecule #1: 40S ribosomal protein SA

• Macromolecule: Name: 40S ribosomal protein SA / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 32.883938 KDa

Sequence

String:

MSGALDVLQM KEEDVLKFLA AGTHLGGTNL DFQMEQYIYK RKSDGIYIIN LKRTWEKLLL AARAIVAIEN PADVSVISSR NTGQRAVLK FAAATGATPI AGRFTPGTFT NQIQAAFREP RLLVVTDPRA DHQPLTEASY VNLPTIALCN TDSPLRYVDI A IPCNNKGA HSVGLMWWML AREVLRMRGT ISREHPWEVM PDLYFYRDPE EIEKEEQAAA EKAVTKEEFQ GEWTAPAPEF TA TQPEVAD WSEGVQVPSV PIQQFPTEDW SAQPATEDWS AAPTAQATEW VGATTDWS

Macromolecule #2: 40S ribosomal protein S3a

• Macromolecule: Name: 40S ribosomal protein S3a / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 30.002061 KDa

Sequence

String:

MAVGKNKRLT KGGKKGAKKK VVDPFSKKDW YDVKAPAMFN IRNIGKTLVT RTQGTKIASD GLKGRVFEVS LADLQNDEVA FRKFKLITE DVQGKNCLTN FHGMDLTRDK MCSMVKKWQT MIEAHVDVKT TDGYLLRLFC VGFTKKRNNQ IRKTSYAQHQ Q VRQIRKKM MEIMTREVQT NDLKEVVNKL IPDSIGKDIE KACQSIYPLH DVFVRKVKML KKPKFELGKL MELHGEGSSS GK ATGDETG AKVERADGYE PPVQESV

Macromolecule #3: 40S ribosomal protein S2

• Macromolecule: Name: 40S ribosomal protein S2 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 31.376516 KDa

Sequence

String:

MADDAGAAGG PGGPGGPGMG NRGGFRGGFG SGIRGRGRGR GRGRGRGRGA RGGKAEDKEW MPVTKLGRLV KDMKIKSLEE IYLFSLPIK ESEIIDFFLG ASLKDEVLKI MPVQKQTRAG QRTRFKAFVA IGDYNGHVGL GVKCSKEVAT AIRGAIILAK L SIVPVRRG YWGNKIGKPH TVPCKVTGRC GSVLVRLIPA PRGTGIVSAP VPKKLLMMAG IDDCYTSARG CTATLGNFAK AT FDAISKT YSYLTPDLWK ETVFTKSPYQ EFTDHLVKTH TRVSVQRTQA PAVATT

Macromolecule #4: 40S ribosomal protein S26

• Macromolecule: Name: 40S ribosomal protein S26 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 13.047532 KDa

Sequence

String:

MTKKRRNNGR AKKGRGHVQP IRCTNCARCV PKDKAIKKFV IRNIVEAAAV RDISEASVFD AYVLPKLYVK LHYCVSCAIH SKVVRNRSR EARKDRTPPP RFRPAGAAPR PPPKPM

Macromolecule #5: 40S ribosomal protein S4, X isoform

• Macromolecule: Name: 40S ribosomal protein S4, X isoform / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 29.654869 KDa

Sequence

String:

MARGPKKHLK RVAAPKHWML DKLTGVFAPR PSTGPHKLRE CLPLIIFLRN RLKYALTGDE VKKICMQRFI KIDGKVRTDI TYPAGFMDV ISIDKTGENF RLIYDTKGRF AVHRITPEEA KYKLCKVRKI FVGTKGIPHL VTHDARTIRY PDPLIKVNDT I QIDLETGK ITDFIKFDTG NLCMVTGGAN LGRIGVITNR ERHPGSFDVV HVKDANGNSF ATRLSNIFVI GKGNKPWISL PR GKGIRLT IAEERDKRLA AKQSSG

Macromolecule #6: 60S ribosomal protein L41

• Macromolecule: Name: 60S ribosomal protein L41 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 3.473451 KDa

Sequence

String:

MRAKWRKKRM RRLKRKRRKM RQRSK

Macromolecule #7: 40S ribosomal protein S6

• Macromolecule: Name: 40S ribosomal protein S6 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 28.751906 KDa

Sequence

String:

MKLNISFPAT GCQKLIEVDD ERKLRTFYEK RMATEVAADA LGEEWKGYVV RISGGNDKQG FPMKQGVLTH GRVRLLLSKG HSCYRPRRT GERKRKSVRG CIVDANLSVL NLVIVKKGEK DIPGLTDTTV PRRLGPKRAS RIRKLFNLSK EDDVRQYVVR K PLNKEGKK PRTKAPKIQR LVTPRVLQHK RRRIALKKQR TKKNKEEAAE YAKLLAKRMK EAKEKRQEQI AKRRRLSSLR AS TSKSESS QK

Macromolecule #8: 40S ribosomal protein S7

• Macromolecule: Name: 40S ribosomal protein S7 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 22.168914 KDa

Sequence

String:

MFSSSAKIVK PNGEKPDEFE SGISQALLEL EMNSDLKAQL RELNITAAKE IEVGGGRKAI IIFVPVPQLK SFQKIQVRLV RELEKKFSG KHVVFIAQRR ILPKPTRKSR TKNKQKRPRS RTLTAVHDAI LEDLVFPSEI VGKRIRVKLD GSRLIKVHLD K AQQNNVEH KVETFSGVYK KLTGKDVNFE FPEFQL

Macromolecule #9: 40S ribosomal protein S8

• Macromolecule: Name: 40S ribosomal protein S8 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 24.263387 KDa

Sequence

String:

MGISRDNWHK RRKTGGKRKP YHKKRKYELG RPAANTKIGP RRIHTVRVRG GNKKYRALRL DVGNFSWGSE CCTRKTRIID VVYNASNNE LVRTKTLVKN CIVLIDSTPY RQWYESHYAL PLGRKKGAKL TPEEEEILNK KRSKKIQKKY DERKKNAKIS S LLEEQFQQ GKLLACIASR PGQCGRADGY VLEGKELEFY LRKIKARKGK

Macromolecule #10: 40S ribosomal protein S9

• Macromolecule: Name: 40S ribosomal protein S9 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 22.641564 KDa

Sequence

String:

MPVARSWVCR KTYVTPRRPF EKSRLDQELK LIGEYGLRNK REVWRVKFTL AKIRKAAREL LTLDEKDPRR LFEGNALLRR LVRIGVLDE GKMKLDYILG LKIEDFLERR LQTQVFKLGL AKSIHHARVL IRQRHIRVRK QVVNIPSFIV RLDSQKHIDF S LRSPYGGG RPGRVKRKNA KKGQGGAGAG DDEEED

Macromolecule #11: 40S ribosomal protein S11

• Macromolecule: Name: 40S ribosomal protein S11 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 18.468826 KDa

Sequence

String:

MADIQTERAY QKQPTIFQNK KRVLLGETGK EKLPRYYKNI GLGFKTPKEA IEGTYIDKKC PFTGNVSIRG RILSGVVTKM KMQRTIVIR RDYLHYIRKY NRFEKRHKNM SVHLSPCFRD VQIGDIVTVG ECRPLSKTVR FNVLKVTKAA GTKKQFQKF

Macromolecule #12: 40S ribosomal protein S13

• Macromolecule: Name: 40S ribosomal protein S13 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 17.259389 KDa

Sequence

String:

MGRMHAPGKG LSQSALPYRR SVPTWLKLTS DDVKEQIYKL AKKGLTPSQI GVILRDSHGV AQVRFVTGNK ILRILKSKGL APDLPEDLY HLIKKAVAVR KHLERNRKDK DAKFRLILIE SRIHRLARYY KTKRVLPPNW KYESSTASAL VA

Macromolecule #13: 40S ribosomal protein S21

• Macromolecule: Name: 40S ribosomal protein S21 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 9.124389 KDa

Sequence

String:

MQNDAGEFVD LYVPRKCSAS NRIIGAKDHA SIQMNVAEVD KVTGRFNGQF KTYAICGAIR RMGESDDSIL RLAKADGIVS KNF

Macromolecule #14: 40S ribosomal protein S15a

• Macromolecule: Name: 40S ribosomal protein S15a / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 14.865555 KDa

Sequence

String:

MVRMNVLADA LKSINNAEKR GKRQVLIRPC SKVIVRFLTV MMKHGYIGEF EIIDDHRAGK IVVNLTGRLN KCGVISPRFD VQLKDLEKW QNNLLPSRQF GFIVLTTSAG IMDHEEARRK HTGGKILGFF F

Macromolecule #15: 40S ribosomal protein S24

• Macromolecule: Name: 40S ribosomal protein S24 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 15.236054 KDa

Sequence

String:

MNDTVTIRTR KFMTNRLLQR KQMVIDVLHP GKATVPKTEI REKLAKMYKT TPDVIFVFGF RTHFGGGKTT GFGMIYDSLD YAKKNEPKH RLARHGLYEK KKTSRKQRKE RKNRMKKVRG TAKANVGAGK KQ

Macromolecule #16: 40S ribosomal protein S27

• Macromolecule: Name: 40S ribosomal protein S27 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 9.480186 KDa

Sequence

String:

MPLAKDLLHP SPEEEKRKHK KKRLVQSPNS YFMDVKCPGC YKITTVFSHA QTVVLCVGCS TVLCQPTGGK ARLTEGCSFR RKQH

Macromolecule #17: 40S ribosomal protein S30

• Macromolecule: Name: 40S ribosomal protein S30 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 6.668938 KDa

Sequence

String:

KVHGSLARAG KVRGQTPKVA KQEKKKKKTG RAKRRMQYNR RFVNVVPTFG KKKGPNANS

Macromolecule #19: 40S ribosomal protein S17

• Macromolecule: Name: 40S ribosomal protein S17 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 15.578156 KDa

Sequence

String:

MGRVRTKTVK KAARVIIEKY YTRLGNDFHT NKRVCEEIAI IPSKKLRNKI AGYVTHLMKR IQRGPVRGIS IKLQEEERER RDNYVPEVS ALDQEIIEVD PDTKEMLKLL DFGSLSNLQV TQPTVGMNFK TPRGPV

Macromolecule #20: 40S ribosomal protein S3

• Macromolecule: Name: 40S ribosomal protein S3 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-(apurinic or apyrimidinic site) lyase
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 26.729369 KDa

Sequence

String:

MAVQISKKRK FVADGIFKAE LNEFLTRELA EDGYSGVEVR VTPTRTEIII LATRTQNVLG EKGRRIRELT AVVQKRFGFP EGSVELYAE KVATRGLCAI AQAESLRYKL LGGLAVRRAC YGVLRFIMES GAKGCEVVVS GKLRGQRAKS MKFVDGLMIH S GDPVNYYV DTAVRHVLLR QGVLGIKVKI MLPWDPTGKI GPKKPLPDHV SIVEPKDEIL PTTPISEQKG GKPEPPAMPQ PV PTA

Macromolecule #21: 40S ribosomal protein S5

• Macromolecule: Name: 40S ribosomal protein S5 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 22.913453 KDa

Sequence

String:

MTEWETAAPA VAETPDIKLF GKWSTDDVQI NDISLQDYIA VKEKYAKYLP HSAGRYAAKR FRKAQCPIVE RLTNSMMMHG RNNGKKLMT VRIVKHAFEI IHLLTGENPL QVLVNAIINS GPREDSTRIG RAGTVRRQAV DVSPLRRVNQ AIWLLCTGAR E AAFRNIKT IAECLADELI NAAKGSSNSY AIKKKDELER VAKSNR

Macromolecule #22: 40S ribosomal protein S10

• Macromolecule: Name: 40S ribosomal protein S10 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 18.933846 KDa

Sequence

String:

MLMPKKNRIA IYELLFKEGV MVAKKDVHMP KHPELADKNV PNLHVMKAMQ SLKSRGYVKE QFAWRHFYWY LTNEGIQYLR DYLHLPPEI VPATLRRSRP ETGRPRPKGL EGERPARLTR GEADRDTYRR SAVPPGADKK AEAGAGSATE FQFRGGFGRG R GQPPQ

Macromolecule #23: 40S ribosomal protein S12

• Macromolecule: Name: 40S ribosomal protein S12 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 14.538987 KDa

Sequence

String:

MAEEGIAAGG VMDVNTALQE VLKTALIHDG LARGIREAAK ALDKRQAHLC VLASNCDEPM YVKLVEALCA EHQINLIKVD DNKKLGEWV GLCKIDREGK PRKVVGCSCV VVKDYGKESQ AKDVIEEYFK CKK

Macromolecule #24: 40S ribosomal protein S15

• Macromolecule: Name: 40S ribosomal protein S15 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 17.076207 KDa

Sequence

String:

MAEVEQKKKR TFRKFTYRGV DLDQLLDMSY EQLMQLYSAR QRRRLNRGLR RKQHSLLKRL RKAKKEAPPM EKPEVVKTHL RDMIILPEM VGSMVGVYNG KTFNQVEIKP EMIGHYLGEF SITYKPVKHG RPGIGATHSS RFIPLK

Macromolecule #25: 40S ribosomal protein S16

• Macromolecule: Name: 40S ribosomal protein S16 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 16.477377 KDa

Sequence

String:

MPSKGPLQSV QVFGRKKTAT AVAHCKRGNG LIKVNGRPLE MIEPRTLQYK LLEPVLLLGK ERFAGVDIRV RVKGGGHVAQ IYAIRQSIS KALVAYYQKY VDEASKKEIK DILIQYDRTL LVADPRRCES KKFGGPGARA RYQKSYR

Macromolecule #26: 40S ribosomal protein S18

• Macromolecule: Name: 40S ribosomal protein S18 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 17.759777 KDa

Sequence

String:

MSLVIPEKFQ HILRVLNTNI DGRRKIAFAI TAIKGVGRRY AHVVLRKADI DLTKRAGELT EDEVERVITI MQNPRQYKIP DWFLNRQKD VKDGKYSQVL ANGLDNKLRE DLERLKKIRA HRGLRHFWGL RVRGQHTKTT GRRGRTVGVS KKK

Macromolecule #27: 40S ribosomal protein S19

• Macromolecule: Name: 40S ribosomal protein S19 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 16.091562 KDa

Sequence

String:

MPGVTVKDVN QQEFVRALAA FLKKSGKLKV PEWVDTVKLA KHKELAPYDE NWFYTRAAST ARHLYLRGGA GVGSMTKIYG GRQRNGVMP SHFSRGSKSV ARRVLQALEG LKMVEKDQDG GRKLTPQGQR DLDRIAGQVA AANKKH

Macromolecule #28: 40S ribosomal protein S20

• Macromolecule: Name: 40S ribosomal protein S20 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 13.398763 KDa

Sequence

String:

MAFKDTGKTP VEPEVAIHRI RITLTSRNVK SLEKVCADLI RGAKEKNLKV KGPVRMPTKT LRITTRKTPC GEGSKTWDRF QMRIHKRLI DLHSPSEIVK QITSISIEPG VEVEVTIADA

Macromolecule #29: 40S ribosomal protein S25

• Macromolecule: Name: 40S ribosomal protein S25 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 13.776224 KDa

Sequence

String:

MPPKDDKKKK DAGKSAKKDK DPVNKSGGKA KKKKWSKGKV RDKLNNLVLF DKATYDKLCK EVPNYKLITP AVVSERLKIR GSLARAALQ ELLSKGLIKL VSKHRAQVIY TRNTKGGDAP AAGEDA

Macromolecule #30: 40S ribosomal protein S28

• Macromolecule: Name: 40S ribosomal protein S28 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 7.855052 KDa

Sequence

String:

MDTSRVQPIK LARVTKVLGR TGSQGQCTQV RVEFMDDTSR SIIRNVKGPV REGDVLTLLE SEREARRLR

Macromolecule #31: 40S ribosomal protein S29

• Macromolecule: Name: 40S ribosomal protein S29 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 6.690821 KDa

Sequence

String:

MGHQQLYWSH PRKFGQGSRS CRVCSNRHGL IRKYGLNMCR QCFRQYAKDI GFIKLD

Macromolecule #32: Ubiquitin-40S ribosomal protein S27a

• Macromolecule: Name: Ubiquitin-40S ribosomal protein S27a / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 18.004041 KDa

Sequence

String:

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGGAKKR KKKSYTTPK KNKHKRKKVK LAVLKYYKVD ENGKISRLRR ECPSDECGAG VFMASHFDRH YCGKCCLTYC FNKPEDK

Macromolecule #33: Receptor of activated protein C kinase 1

• Macromolecule: Name: Receptor of activated protein C kinase 1 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 35.115652 KDa

Sequence

String:

MTEQMTLRGT LKGHNGWVTQ IATTPQFPDM ILSASRDKTI IMWKLTRDET NYGIPQRALR GHSHFVSDVV ISSDGQFALS GSWDGTLRL WDLTTGTTTR RFVGHTKDVL SVAFSSDNRQ IVSGSRDKTI KLWNTLGVCK YTVQDESHSE WVSCVRFSPN S SNPIIVSC GWDKLVKVWN LANCKLKTNH IGHTGYLNTV TVSPDGSLCA SGGKDGQAML WDLNEGKHLY TLDGGDIINA LC FSPNRYW LCAATGPSIK IWDLEGKIIV DELKQEVIST SSKAEPPQCT SLAWSADGQT LFAGYTDNLV RVWQVTIGTR

Macromolecule #34: 40S ribosomal protein S14

• Macromolecule: Name: 40S ribosomal protein S14 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 16.302772 KDa

Sequence

String:

MAPRKGKEKK EEQVISLGPQ VAEGENVFGV CHIFASFNDT FVHVTDLSGK ETICRVTGGM KVKADRDESS PYAAMLAAQD VAQRCKELG ITALHIKLRA TGGNRTKTPG PGAQSALRAL ARSGMKIGRI EDVTPIPSDS TRRKGGRRGR RL

Macromolecule #35: 40S ribosomal protein S23

• Macromolecule: Name: 40S ribosomal protein S23 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 15.844666 KDa

Sequence

String:

MGKCRGLRTA RKLRSHRRDQ KWHDKQYKKA HLGTALKANP FGGASHAKGI VLEKVGVEAK QPNSAIRKCV RVQLIKNGKK ITAFVPNDG CLNFIEENDE VLVAGFGRKG HAVGDIPGVR FKVVKVANVS LLALYKGKKE RPRS

Macromolecule #36: Eukaryotic translation initiation factor 1A, X-chromosomal

• Macromolecule: Name: Eukaryotic translation initiation factor 1A, X-chromosomal; type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 12.946941 KDa

Sequence

String:

MPKNKGKGGK NRRRGKNENE SEKRELVFKE DGQEYAQVIK MLGNGRLEAM CFDGVKRLCH IRGKLRKKVW INTSDIILVG LRDYQDNKA DVILKYNADE ARSLKAYGEL PEH

Macromolecule #37: Eukaryotic translation initiation factor 3 subunit I

• Macromolecule: Name: Eukaryotic translation initiation factor 3 subunit I / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 36.543773 KDa

Sequence

String:

MKPILLQGHE RSITQIKYNR EGDLLFTVAK DPIVNVWYSV NGERLGTYMG HTGAVWCVDA DWDTKHVLTG SADNSCRLWD CETGKQLAL LKTNSAVRTC GFDFGGNIIM FSTDKQMGYQ CFVSFFDLRD PSQIDNNEPY MKIPCNDSKI TSAVWGPLGE C IIAGHESG ELNQYSAKSG EVLVNVKEHS RQINDIQLSR DMTMFVTASK DNTAKLFDST TLEHQKTFRT ERPVNSAALS PN YDHVVLG GGQEAMDVTT TSTRIGKFEA RFFHLAFEEE FGRVKGHFGP INSVAFHPDG KSYSSGGEDG YVRIHYFDPQ YFE FEFEA

Macromolecule #38: Eukaryotic translation initiation factor 3 subunit B

• Macromolecule: Name: Eukaryotic translation initiation factor 3 subunit B / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 92.593414 KDa

Sequence

String:

MQDAENVAVP EAAEERAEPG QQQPAAEPPP AEGLLRPAGP GAPEAAGTEA SSEEVGIAEA GPESEVRTEP AAEAEAASGP SESPSPPAA EELPGSHAEP PVPAQGEAPG EQARDERSDS RAQAVSEDAG GNEGRAAEAE PRALENGDAD EPSFSDPEDF V DDVSEEEL LGDVLKDRPQ EADGIDSVIV VDNVPQVGPD RLEKLKNVIH KIFSKFGKIT NDFYPEEDGK TKGYIFLEYA SP AHAVDAV KNADGYKLDK QHTFRVNLFT DFDKYMTISD EWDIPEKQPF KDLGNLRYWL EEAECRDQYS VIFESGDRTS IFW NDVKDP VSIEERARWT ETYVRWSPKG TYLATFHQRG IALWGGEKFK QIQRFSHQGV QLIDFSPCER YLVTFSPLMD TQDD PQAII IWDILTGHKK RGFHCESSAH WPIFKWSHDG KFFARMTLDT LSIYETPSMG LLDKKSLKIS GIKDFSWSPG GNIIA FWVP EDKDIPARVT LMQLPTRQEI RVRNLFNVVD CKLHWQKNGD YLCVKVDRTP KGTQGVVTNF EIFRMREKQV PVDVVE MKE TIIAFAWEPN GSKFAVLHGE APRISVSFYH VKNNGKIELI KMFDKQQANT IFWSPQGQFV VLAGLRSMNG ALAFVDT SD CTVMNIAEHY MASDVEWDPT GRYVVTSVSW WSHKVDNAYW LWTFQGRLLQ KNNKDRFCQL LWRPRPPTLL SQEQIKQI K KDLKKYSKIF EQKDRLSQSK ASKELVERRR TMMEDFRKYR KMAQELYMEQ KNERLELRGG VDTDELDSNV DDWEEETIE FFVTEEIIPL GNQE

Macromolecule #39: Eukaryotic translation initiation factor 3 subunit A

• Macromolecule: Name: Eukaryotic translation initiation factor 3 subunit A / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 166.903781 KDa

Sequence

String:

MPAYFQRPEN ALKRANEFLE VGKKQPALDV LYDVMKSKKH RTWQKIHEPI MLKYLELCVD LRKSHLAKEG LYQYKNICQQ VNIKSLEDV VRAYLKMAEE KTEAAKEESQ QMVLDIEDLD NIQTPESVLL SAVSGEDTQD RTDRLLLTPW VKFLWESYRQ C LDLLRNNS RVERLYHDIA QQAFKFCLQY TRKAEFRKLC DNLRMHLSQI QRHHNQSTAI NLNNPESQSM HLETRLVQLD SA ISMELWQ EAFKAVEDIH GLFSLSKKPP KPQLMANYYN KVSTVFWKSG NALFHASTLH RLYHLSREMR KNLTQDEMQR MST RVLLAT LSIPITPERT DIARLLDMDG IIVEKQRRLA TLLGLQAPPT RIGLINDMVR FNVLQYVVPE VKDLYNWLEV EFNP LKLCE RVTKVLNWVR EQPEKEPELQ QYVPQLQNNT ILRLLQQVSQ IYQSIEFSRL TSLVPFVDAF QLERAIVDAA RHCDL QVRI DHTSRTLSFG SDLNYATRED APIGPHLQSM PSEQIRNQLT AMSSVLAKAL EVIKPAHILQ EKEEQHQLAV TAYLKN SRK EHQRILARRQ TIEERKERLE SLNIQREKEE LEQREAELQK VRKAEEERLR QEAKEREKER ILQEHEQIKK KTVRERL EQ IKKTELGAKA FKDIDIEDLE ELDPDFIMAK QVEQLEKEKK ELQERLKNQE KKIDYFERAK RLEEIPLIKS AYEEQRIK D MDLWEQQEEE RITTMQLERE KALEHKNRMS RMLEDRDLFV MRLKAARQSV YEEKLKQFEE RLAEERHNRL EERKRQRKE ERRITYYREK EEEEQRRAEE QMLKEREERE RAERAKREEE LREYQERVKK LEEVERKKRQ RELEIEERER RREEERRLGD SSLSRKDSR WGDRDSEGTW RKGPEADSEW RRGPPEKEWR RGEGRDEDRS HRRDEERPRR LGDDEDREPS LRPDDDRVPR R GMDDDRGP RRGPEEDRFS RRGADDDRPS WRNTDDDRPP RRIADEDRGN WRHADDDRPP RRGLDEDRGS WRTADEDRGP RR GMDDDRG PRRGGADDER SSWRNADDDR GPRRGLDDDR GPRRGMDDDR GPRRGMDDDR GPRRGMDDDR GPRRGLDDDR GPW RNADDD RIPRRGAEDD RGPWRNMDDD RLSRRADDDR FPRRGDDSRP GPWRPLVKPG GWREKEKARE ESWGPPRESR PSEE REWDR EKERDRDNQD REENDKDPER ERDRERDVDR EDRFRRPRDE GGWRRGPAEE SSSWRDSSRR DDRDRDDRRR ERDDR RDLR ERRDLRDDRD RRGPPLRSER EEVSSWRRAD DRKDDRVEER DPPRRVPPPA LSRDRERDRD REREGEKEKA SWRAEK DRE SLRRTKNETD EDGWTTVRR

Macromolecule #40: Eukaryotic translation initiation factor 3 subunit C,Eukaryotic t...

• Macromolecule: Name: Eukaryotic translation initiation factor 3 subunit C,Eukaryotic translation initiation factor 3 subunit C,Eukaryotic translation initiation factor 3 subunit C; type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 106.184797 KDa

Sequence

String:

MSRFFTTGSD SESESSLSGE ELVTKPVGGN YGKQPLLLSE DEEDTKRVVR SAKDKRFEEL TNLIRTIRNA MKIRDVTKCL EEFELLGKA YGKAKSIVDK EGVPRFYIRI LADLEDYLNE LWEDKEGKKK MNKNNAKALS TLRQKIRKYN RDFESHITSY K QNPEQSAD EDAEKNEEDS EGSSDEDEDE DGVSAATFLK KKSEAPSGES RKFLKKMDDE DEDSEDSEDD EDWDTGSTSS DS DSEEEEG KQTALASRFL KKAPTTDEDK KAAEKKREDK AKKKHDRKSK RLDEEEEDNE GGEWERVRG(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)GV PLVKEKPKMF AKGTEITHAV VIKKLNEILQ ARGKKGTDRA AQIELLQL L VQIAAENNLG EGVIVKIKFN IIASLYDYNP NLATYMKPEM WGKCLDCINE LMDILFANPN IFVGENILEE SENLHNADQ PLRVRGCILT LVERMDEEFT KIMQNTDPHS QEYVEHLKDE AQVCAIIERV QRYLEEKGTT EEVCRIYLLR ILHTYYKFDY KAHQRQLTP PEGSSKSEQD QAENEGEDSA VLMERLCKYI YAKDRTDRIR TCAILCHIYH HALHSRWYQA RDLMLMSHLQ D NIQHADPP VQILYNRTMV QLGICAFRQG LTKDAHNALL DIQSSGRAKE LLGQGLLLRS LQERNQEQEK VERRRQVPFH LH INLELLE CVYLVSAMLL EIPYMAAHES DARRRMISKQ FHHQLRVGER QPLLGPPESM REHVVAASKA MKMGDWKTCH SFI INEKMN GKVWDLFPEA DKVRTMLVRK IQEESLRTYL FTYSSVYDSI SMETLSDMFE LDLPTVHSII SKMIINEELM ASLD QPTQT VVMHRTEPTA QQNLALQLAE KLGSLVENNE RVFDHKQGTY GGYFRDQKDG YRKNEGYMRR GGYRQQQSQT AY

Macromolecule #41: Eukaryotic translation initiation factor 3 subunit E

• Macromolecule: Name: Eukaryotic translation initiation factor 3 subunit E / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 52.281633 KDa

Sequence

String:

MAEYDLTTRI AHFLDRHLVF PLLEFLSVKE IYNEKELLQG KLDLLSDTNM VDFAMDVYKN LYSDDIPHAL REKRTTVVAQ LKQLQAETE PIVKMFEDPE TTRQMQSTRD GRMLFDYLAD KHGFRQEYLD TLYRYAKFQY ECGNYSGAAE YLYFFRVLVP A TDRNALSS LWGKLASEIL MQNWDAAMED LTRLKETIDN NSVSSPLQSL QQRTWLIHWS LFVFFNHPKG RDNIIDLFLY QP QYLNAIQ TMCPHILRYL TTAVITNKDV RKRRQVLKDL VKVIQQESYT YKDPITEFVE CLYVNFDFDG AQKKLRECES VLV NDFFLV ACLEDFIENA RLFIFETFCR IHQCISINML ADKLNMTPEE AERWIVNLIR NARLDAKIDS KLGHVVMGNN AVSP YQQVI EKTKSLSFRS QMLAMNIEKK LNQNSRSEAP NWATQDSGFY

Macromolecule #42: Eukaryotic translation initiation factor 3 subunit F

• Macromolecule: Name: Eukaryotic translation initiation factor 3 subunit F / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO / EC number: ubiquitinyl hydrolase 1
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 37.593645 KDa

Sequence

String:

MATPAVPVSA PPATPTPVPA AAPASVPAPT PAPAAAPVPA AAPASSSDPA AAAAATAAPG QTPASAQAPA QTPAPALPGP ALPGPFPGG RVVRLHPVIL ASIVDSYERR NEGAARVIGT LLGTVDKHSV EVTNCFSVPH NESEDEVAVD MEFAKNMYEL H KKVSPNEL ILGWYATGHD ITEHSVLIHE YYSREAPNPI HLTVDTSLQN GRMSIKAYVS TLMGVPGRTM GVMFTPLTVK YA YYDTERI GVDLIMKTCF SPNRVIGLSS DLQQVGGASA RIQDALSTVL QYAEDVLSGK VSADNTVGRF LMSLVNQVPK IVP DDFETM LNSNINDLLM VTYLANLTQS QIALNEKLVN L

Macromolecule #43: Eukaryotic translation initiation factor 3 subunit H

• Macromolecule: Name: Eukaryotic translation initiation factor 3 subunit H / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 39.979277 KDa

Sequence

String:

MASRKEGTGS TATSSSSTAG AAGKGKGKGG SGDSAVKQVQ IDGLVVLKII KHYQEEGQGT EVVQGVLLGL VVEDRLEITN CFPFPQHTE DDADFDEVQY QMEMMRSLRH VNIDHLHVGW YQSTYYGSFV TRALLDSQFS YQHAIEESVV LIYDPIKTAQ G SLSLKAYR LTPKLMEVCK EKDFSPEALK KANITFEYMF EEVPIVIKNS HLINVLMWEL EKKSAVADKH ELLSLASSNH LG KNLQLLM DRVDEMSQDI VKYNTYMRNT SKQQQQKHQY QQRRQQENMQ RQSRGEPPLP EEDLSKLFKP PQPPARMDSL LIA GQINTY CQNIKEFTAQ NLGKLFMAQA LQEYNN

Macromolecule #44: Eukaryotic translation initiation factor 3 subunit K

• Macromolecule: Name: Eukaryotic translation initiation factor 3 subunit K / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 25.083619 KDa

Sequence

String:

MAMFEQMRAN VGKLLKGIDR YNPENLATLE RYVETQAKEN AYDLEANLAV LKLYQFNPAF FQTTVTAQIL LKALTNLPHT DFTLCKCMI DQAHQEERPI RQILYLGDLL ETCHFQAFWQ ALDENMDLLE GITGFEDSVR KFICHVVGIT YQHIDRWLLA E MLGDLSDS QLKVWMSKYG WSADESGQIF ICSQEESIKP KNIVEKIDFD SVSSIMASSQ

Macromolecule #45: Eukaryotic translation initiation factor 3 subunit L

• Macromolecule: Name: Eukaryotic translation initiation factor 3 subunit L / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 66.803734 KDa

Sequence

String:

MSYPADDYES EAAYDPYAYP SDYDMHTGDP KQDLAYERQY EQQTYQVIPE VIKNFIQYFH KTVSDLIDQK VYELQASRVS SDVIDQKVY EIQDIYENSW TKLTERFFKN TPWPEAEAIA PQVGNDAVFL ILYKELYYRH IYAKVSGGPS LEQRFESYYN Y CNLFNYIL NADGPAPLEL PNQWLWDIID EFIYQFQSFS QYRCKTAKKS EEEIDFLRSN PKIWNVHSVL NVLHSLVDKS NI NRQLEVY TSGGDPESVA GEYGRHSLYK MLGYFSLVGL LRLHSLLGDY YQAIKVLENI ELNKKSMYSR VPECQVTTYY YVG FAYLMM RRYQDAIRVF ANILLYIQRT KSMFQRTTYK YEMINKQNEQ MHALLAIALT MYPMRIDESI HLQLREKYGD KMLR MQKGD PQVYEELFSY SCPKFLSPVV PNYDNVHPNY HKEPFLQQLK VFSDEVQQQA QLSTIRSFLK LYTTMPVAKL AGFLD LTEQ EFRIQLLVFK HKMKNLVWTS GISALDGEFQ SASEVDFYID KDMIHIADTK VARRYGDFFI RQIHKFEELN RTLKKM GQR P

Macromolecule #46: Eukaryotic translation initiation factor 3 subunit M

• Macromolecule: Name: Eukaryotic translation initiation factor 3 subunit M / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 42.555832 KDa

Sequence

String:

MSVPAFIDIS EEDQAAELRA YLKSKGAEIS EENSEGGLHV DLAQIIEACD VCLKEDDKDV ESVMNSVVSL LLILEPDKQE ALIESLCEK LVKFREGERP SLRLQLLSNL FHGMDKNTPV RYTVYCSLIK VAASCGAIQY IPTELDQVRK WISDWNLTTE K KHTLLRLL YEALVDCKKS DAASKVMVEL LGSYTEDNAS QARVDAHRCI VRALKDPNAF LFDHLLTLKP VKFLEGELIH DL LTIFVSA KLASYVKFYQ NNKDFIDSLG LLHEQNMAKM RLLTFMGMAV ENKEISFDTM QQELQIGADD VEAFVIDAVR TKM VYCKID QTQRKVVVSH STHRTFGKQQ WQQLYDTLNA WKQNLNKVKN SLLSLSDT

Macromolecule #47: ATP-binding cassette sub-family E member 1

• Macromolecule: Name: ATP-binding cassette sub-family E member 1 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 67.405234 KDa

Sequence

String:

MADKLTRIAI VNHDKCKPKK CRQECKKSCP VVRMGKLCIE VTPQSKIAWI SETLCIGCGI CIKKCPFGAL SIVNLPSNLE KETTHRYCA NAFKLHRLPI PRPGEVLGLV GTNGIGKSTA LKILAGKQKP NLGKYDDPPD WQEILTYFRG SELQNYFTKI L EDDLKAII KPQYVDQIPK AAKGTVGSIL DRKDETKTQA IVCQQLDLTH LKERNVEDLS GGELQRFACA VVCIQKADIF MF DEPSSYL DVKQRLKAAI TIRSLINPDR YIIVVEHDLS VLDYLSDFIC CLYGVPSAYG VVTMPFSVRE GINIFLDGYV PTE NLRFRD ASLVFKVAET ANEEEVKKMC MYKYPGMKKK MGEFELAIVA GEFTDSEIMV MLGENGTGKT TFIRMLAGRL KPDE GGEVP VLNVSYKPQK ISPKSTGSVR QLLHEKIRDA YTHPQFVTDV MKPLQIENII DQEVQTLSGG ELQRVALALC LGKPA DVYL IDEPSAYLDS EQRLMAARVV KRFILHAKKT AFVVEHDFIM ATYLADRVIV FDGVPSKNTV ANSPQTLLAG MNKFLS QLE ITFRRDPNNY RPRINKLNSI KDVEQKKSGN YFFLDD

Macromolecule #48: Eukaryotic translation initiation factor 3 subunit D

• Macromolecule: Name: Eukaryotic translation initiation factor 3 subunit D / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 64.060758 KDa

Sequence

String:

MAKFMTPVIQ DNPSGWGPCA VPEQFRDMPY QPFSKGDRLG KVADWTGATY QDKRYTNKYS SQFGGGSQYA YFHEEDESSF QLVDTARTQ KTAYQRNRMR FAQRNLRRDK DRRNMLQFNL QILPKSAKQK ERERIRLQKK FQKQFGVRQK WDQKSQKPRD S SVEVRSDW EVKEEMDFPQ LMKMRYLEVS EPQDIECCGA LEYYDKAFDR ITTRSEKPLR SIKRIFHTVT TTDDPVIRKL AK TQGNVFA TDAILATLMS CTRSVYSWDI VVQRVGSKLF FDKRDNSDFD LLTVSETANE PPQDEGNSFN SPRNLAMEAT YIN HNFSQQ CLRMGKERYN FPNPNPFVED DMDKNEIASV AYRYRRWKLG DDIDLIVRCE HDGVMTGANG EVSFINIKTL NEWD SRHCN GVDWRQKLDS QRGAVIATEL KNNSYKLARW TCCALLAGSE YLKLGYVSRY HVKDSSRHVI LGTQQFKPNE FASQI NLSV ENAWGILRCV IDICMKLEEG KYLILKDPNK QVIRVYSLPD GTFSSDEDEE EEEEEEEEEE EEET

Macromolecule #49: RNA recognition motif

• Macromolecule: Name: RNA recognition motif / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 5.562077 KDa

Sequence

String:

AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAA

Macromolecule #51: Eukaryotic translation initiation factor 2 subunit 2

• Macromolecule: Name: Eukaryotic translation initiation factor 2 subunit 2 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 38.454484 KDa

Sequence

String:

MSGDEMIFDP TMSKKKKKKK KPFMLDEEGD TQTEETQPSE TKEVEPEPTE DKDLEADEED TRKKDASDDL DDLNFFNQKK KKKKTKKIF DIDEAEEGVK DLKIESDVQE PTEPEDDLDI MLGNKKKKKK NVKFPDEDEI LEKDEALEDE DNKKDDGISF S NQTGPAWA GSERDYTYEE LLNRVFNIMR EKNPDMVAGE KRKFVMKPPQ VVRVGTKKTS FVNFTDICKL LHRQPKHLLA FL LAELGTS GSIDGNNQLV IKGRFQQKQI ENVLRRYIKE YVTCHTCRSP DTILQKDTRL YFLQCETCHS RCSVASIKTG FQA VTGKRA QLRAKAN

Macromolecule #52: Eukaryotic translation initiation factor 2 subunit 1

• Macromolecule: Name: Eukaryotic translation initiation factor 2 subunit 1 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 36.16118 KDa

Sequence

String:

MPGLSCRFYQ HKFPEVEDVV MVNVRSIAEM GAYVSLLEYN NIEGMILLSE LSRRRIRSIN KLIRIGRNEC VVVIRVDKEK GYIDLSKRR VSPEEAIKCE DKFTKSKTVY SILRHVAEVL EYTKDEQLES LFQRTAWVFD DKYKRPGYGA YDAFKHAVSD P SILDSLDL NEDEREVLIN NINRRLTPQA VKIRADIEVA CYGYEGIDAV KEALRAGLNC STENMPIKIN LIAPPRYVMT TT TLERTEG LSVLSQAMAV IKEKIEEKRG VFNVQMEPKV VTDTDETELA RQMERLEREN AEVDGDDDAE EMEAKAED

Macromolecule #53: Eukaryotic translation initiation factor 2 subunit 3

• Macromolecule: Name: Eukaryotic translation initiation factor 2 subunit 3 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO / EC number: protein-synthesizing GTPase
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 51.178406 KDa

Sequence

String:

MAGGEAGVTL GQPHLSRQDL TTLDVTKLTP LSHEVISRQA TINIGTIGHV AHGKSTVVKA ISGVHTVRFK NELERNITIK LGYANAKIY KLDDPSCPRP ECYRSCGSST PDEFPTDIPG TKGNFKLVRH VSFVDCPGHD ILMATMLNGA AVMDAALLLI A GNESCPQP QTSEHLAAIE IMKLKHILIL QNKIDLVKES QAKEQYEQIL AFVQGTVAEG APIIPISAQL KYNIEVVCEY IV KKIPVPP RDFTSEPRLI VIRSFDVNKP GCEVDDLKGG VAGGSILKGV LKVGQEIEVR PGIVSKDSEG KLMCKPIFSK IVS LFAEHN DLQYAAPGGL IGVGTKIDPT LCRADRMVGQ VLGAVGALPE IFTELEISYF LLRRLLGVRT EGDKKAAKVQ KLSK NEVLM VNIGSLSTGG RVSAVKADLG KIVLTNPVCT EVGEKIALSR RVEKHWRLIG WGQIRRGVTI KPTVDDD

Macromolecule #54: Eukaryotic translation initiation factor 1

• Macromolecule: Name: Eukaryotic translation initiation factor 1 / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 12.752498 KDa

Sequence

String:

MSAIQNLHSF DPFADASKGD DLLPAGTEDY IHIRIQQRNG RKTLTTVQGI ADDYDKKKLV KAFKKKFACN GTVIEHPEYG EVIQLQGDQ RKNICQFLVE IGLAKDDQLK VHGF

Macromolecule #18: 18S ribosomal RNA

• Macromolecule: Name: 18S ribosomal RNA / type: rna / ID: 18 / Number of copies: 1
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 555.515875 KDa

Sequence

String:

UACCUGGUUG AUCCUGCCAG UAGCAUAUGC UUGUCUCAAA GAUUAAGCCA UGCAUGUCUA AGUACGCACG GCCGGUACAG UGAAACUGC GAAUGGCUCA UUAAAUCAGU UAUGGUUCCU UUGGUCGCUC GUACUUGGAU AACUGUGGUA AUUCUAGAGC U AAUACAUG CCGACGGGCG CUGACCCCCU UCGCGGGGGG GAUGCGUGCA UUUAUCAGUG GUGACUCUAG AUAACCUCGG GC CGAUCGC ACGCCGCGGC GACGACCCAU UCGAACGUCU GCCCUAUCAA CUUUCGAUGG UAGUCGCCGU GCCUACCAUG GUG ACCACG GGUGACGGGG AAUCAGGGUU CGAUUCCGGA GAGGGAGCCU GAGAAACGGC UACCACAUCC AAGGAAGGCA GCAG GCGCG CAAAUUACCC ACUCCCGACC CGGGGAGGUA GUGACGAAAA AUAACAAUAC AGGACUCUUU CGAGGCCCUG UAAUU GGAA UGAGUCCACU UUAAAUCCUU UAACGAGGAU CCAUUGGAGG GCAAGUCUGG UGCCAGCAGC CGCGGUAAUU CCAGCU CCA AUAGCGUAUA UUAAAGUUGC UGCAGUUAAA AAGCUCGUAG UUGGAUCUUG GGAGCGGGCG GGCGGUCCGC CGCGAGG CG AGCCACCGCC CGUCCCCGCC CUCUCGGCCG AAGCGUUUAC UUUGAAAAAA UUAGAGUGUU CAAAGCAGGC CGCCUGGA U ACCGCAGCUA GGAAUAAUGG AAUAGGACCG CGGUUCUAUU UUGUUGGUUU UCGGAACUGA GGCCAUGAUU AAGAGGGAC GGCCGGGGGC AUUCGUAUUG CGCCGCUAGA GGUGAAAUUC UUGGACCGGC GCAAGACGGA CCAGAGCGAA AGCAUUUGCC AAGAAUGUU UUCAUUAAUC AAGAACGAAA GUCGGAGGUU CGAAGACGAU CAGAUACCGU CGUAGUUCCG ACCAUAAACG A UGCCGACC GGCGAUGCGG CGGCGUUAUU CCCAUGACCC GCCGGGCAGC UUCCGGGAAA CCAAAGUCUU UGGGUUCCGG GG GGAGUAU GGUUGCAAAG CUGAAACUUA AAGGAAUUGA CGGAAGGGCA CCACCAGGAG UGGAGCCUGC GGCUUAAUUU GAC UCAACA CGGGAAACCU CACCCGGCCC GGACACGGAC AGGAUUGACA GAUUGAUAGC UCUUUCUCGA UUCCGUGGGU GGUG GUGCA UGGCCGUUCU UAGUUGGUGG AGCGAUUUGU CUGGUUAAUU CCGAUAACGA ACGAGACUCU GGCAUGCUAA CUAGU UACG CGACCGGUCG GCGUAACUUC UUAGAGGGAC AAGUGGCGUU CAGCCACCCG AGAUUGAGCA AUAACAGGUC UGUGAU GCC CUUAGAUGUC CGGGGCUGCA CGCGCGCUAC ACUGACUGGC UCAGCGUGUG CCUACCCUAC GCCGGCAGGC GCGGGUA AC CCGUUGAACC CCAUUCGUGA UGGGGAUCGG GGAUUGCAAU UAUUCCCCAU GAACGAGGAA UUCCCAGUAA GUGCGGGU C AUAAGCUUGC GUUGAUUAAG UCCCUGCCCU UUGUACACAC CGCCCGUCGC UACUACCGAU UGGAUGGUUU AGUGAGGCC CUCGGAUCGG CCCCGCCGGG GUGCCCUGGC GGAGCGCUGA GAAGACGGUC GAACUUGACU AUCUAGAGGA AGUAAAAGUC GUAACAAGG UUUCCGUAGG UAAACCUGCG GAAGGAUCAU UA

Macromolecule #50: tRNA

• Macromolecule: Name: tRNA / type: rna / ID: 50 / Number of copies: 1
• Source (natural): Organism: Human (human)
• Molecular weight: Theoretical: 24.21551 KDa

Sequence

String:

AGCAGAGUAG CGCAGCGGAA GCGUGCUGGG CCCAUAACCC AGAGGUCGAU GGAUCGAAAC CAUCCUCUGC UACCA

Macromolecule #55: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 55 / Number of copies: 4 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #56: IRON/SULFUR CLUSTER

• Macromolecule: Name: IRON/SULFUR CLUSTER / type: ligand / ID: 56 / Number of copies: 2 / Formula: SF4
• Molecular weight: Theoretical: 351.64 Da

Chemical component information

ChemComp-FS1:
IRON/SULFUR CLUSTER

Macromolecule #57: ADENOSINE-5'-DIPHOSPHATE

• Macromolecule: Name: ADENOSINE-5'-DIPHOSPHATE / type: ligand / ID: 57 / Number of copies: 1 / Formula: ADP
• Molecular weight: Theoretical: 427.201 Da

Chemical component information

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

Macromolecule #58: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 58 / Number of copies: 3 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #59: ADENOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 59 / Number of copies: 1 / Formula: ATP
• Molecular weight: Theoretical: 507.181 Da

Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

Macromolecule #60: METHIONINE

• Macromolecule: Name: METHIONINE / type: ligand / ID: 60 / Number of copies: 1 / Formula: MET
• Molecular weight: Theoretical: 149.211 Da

Chemical component information

ChemComp-MET:
METHIONINE

Macromolecule #61: GUANOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 61 / Number of copies: 1 / Formula: GTP
• Molecular weight: Theoretical: 523.18 Da

Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.6
• Vitrification: Cryogen name: ETHANE

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Image recording: Film or detector model: GATAN K2 QUANTUM (4k x 4k) / Average electron dose: 48.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 3.5 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 8712
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD
• Final angle assignment: Type: MAXIMUM LIKELIHOOD