- EMDB-11311: Structure of the Salmonella PrgI needle filament attached to the ... -
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基本情報
登録情報
データベース: EMDB / ID: EMD-11311
タイトル
Structure of the Salmonella PrgI needle filament attached to the basal body
マップデータ
試料
細胞器官・細胞要素: Needle filament of the type III secretion system
タンパク質・ペプチド: PrgI
キーワード
Type 3 secretion system / Salmonella / Helical reconstruction / Needle filament. / PROTEIN TRANSPORT
機能・相同性
機能・相同性情報
type III protein secretion system complex / protein secretion by the type III secretion system / cell surface / extracellular region / identical protein binding 類似検索 - 分子機能
Type III secretion, needle-protein-like / Type III secretion, needle-protein-like superfamily / Type III secretion needle MxiH, YscF, SsaG, EprI, PscF, EscF / Type III secretion system, needle protein 類似検索 - ドメイン・相同性
SPI-1 type 3 secretion system needle filament protein 類似検索 - 構成要素
ジャーナル: Biochem Biophys Rep / 年: 2021 タイトル: Helical reconstruction of and needle filaments attached to type 3 basal bodies. 著者: Vadim Kotov / Michele Lunelli / Jiri Wald / Michael Kolbe / Thomas C Marlovits / 要旨: Gram-negative pathogens evolved a syringe-like nanomachine, termed type 3 secretion system, to deliver protein effectors into the cytoplasm of host cells. An essential component of this system is a ...Gram-negative pathogens evolved a syringe-like nanomachine, termed type 3 secretion system, to deliver protein effectors into the cytoplasm of host cells. An essential component of this system is a long helical needle filament that protrudes from the bacterial surface and connects the cytoplasms of the bacterium and the eukaryotic cell. Previous structural research was predominantly focused on reconstituted type 3 needle filaments, which lacked the biological context. In this work we introduce a facile procedure to obtain high-resolution cryo-EM structure of needle filaments attached to the basal body of type 3 secretion systems. We validate our approach by solving the structure of PrgI filament and demonstrate its utility by obtaining the first high-resolution cryo-EM reconstruction of Shigella MxiH filament. Our work paves the way to systematic structural characterization of attached type 3 needle filaments in the context of mutagenesis studies, protein structural evolution and drug development.
全体 : Needle filament of the type III secretion system
全体
名称: Needle filament of the type III secretion system
要素
細胞器官・細胞要素: Needle filament of the type III secretion system
タンパク質・ペプチド: PrgI
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超分子 #1: Needle filament of the type III secretion system
超分子
名称: Needle filament of the type III secretion system / タイプ: organelle_or_cellular_component / ID: 1 / 親要素: 0 / 含まれる分子: all 詳細: Structure obtained from needles attached to the basal body