Journal: MicroPubl Biol / Year: 2021 Title: CryoEM reveals BIN1 (isoform 8) does not bind to single actin filaments . Authors: Zuoneng Wang / Carsten Mim / Abstract: Cells change their appearance by a concerted action of the cytoskeleton and the plasma membrane. The machinery that bends the membrane includes Bin/Amphiphysin/Rvs (BAR) domain proteins. Recently BAR ...Cells change their appearance by a concerted action of the cytoskeleton and the plasma membrane. The machinery that bends the membrane includes Bin/Amphiphysin/Rvs (BAR) domain proteins. Recently BAR domain proteins garnered attention as actin regulators, either by recruiting actin regulating proteins or through binding to actin directly. BIN1 (an important protein in Alzheimer's Disease, heart disease and cancer) is one of the few BAR proteins that bind to actin directly. Here, we imaged a complex of BIN1 and actin with cryoEM. Our results reveal that BIN1 cannot be found on single actin filaments.
History
Deposition
Apr 14, 2021
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Header (metadata) release
Jun 2, 2021
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Map release
Jun 2, 2021
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Update
Jun 23, 2021
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Current status
Jun 23, 2021
Processing site: PDBe / Status: Released
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Structure visualization
Movie
Surface view with section colored by density value
from 4621 only 837 were chosen based on ice contamination, empty grids, missaligned
Final reconstruction
Number classes used: 1 Applied symmetry - Helical parameters - Δz: 27.657 Å Applied symmetry - Helical parameters - Δ&Phi: -166.594 ° Applied symmetry - Helical parameters - Axial symmetry: C1 (asymmetric) Resolution.type: BY AUTHOR / Resolution: 3.25 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 3.1) Details: for the refinement 3 ab initio models were created with 519748 particles. The most detailed model was refined Number images used: 180008
CTF correction
Software - Name: CTFFIND (ver. 4.1)
Segment selection
Number selected: 11817781
Startup model
Type of model: INSILICO MODEL / In silico model: Ab initio model in cryosparc
Final angle assignment
Type: NOT APPLICABLE Details: Refinement with twist value of -166.61 and rise 27.4
FSC plot (resolution estimation)
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