Phage major tail protein TP901-1 / Phage tail tube protein / Fibronectin type III domain / Fibronectin type 3 domain / Fibronectin type-III domain profile. / Fibronectin type III / Fibronectin type III superfamily / Immunoglobulin-like fold 類似検索 - ドメイン・相同性
ジャーナル: Nat Commun / 年: 2020 タイトル: Architecture of the flexible tail tube of bacteriophage SPP1. 著者: Maximilian Zinke / Katrin A A Sachowsky / Carl Öster / Sophie Zinn-Justin / Raimond Ravelli / Gunnar F Schröder / Michael Habeck / Adam Lange / 要旨: Bacteriophage SPP1 is a double-stranded DNA virus of the Siphoviridae family that infects the bacterium Bacillus subtilis. This family of phages features a long, flexible, non-contractile tail that ...Bacteriophage SPP1 is a double-stranded DNA virus of the Siphoviridae family that infects the bacterium Bacillus subtilis. This family of phages features a long, flexible, non-contractile tail that has been difficult to characterize structurally. Here, we present the atomic structure of the tail tube of phage SPP1. Our hybrid structure is based on the integration of structural restraints from solid-state nuclear magnetic resonance (NMR) and a density map from cryo-EM. We show that the tail tube protein gp17.1 organizes into hexameric rings that are stacked by flexible linker domains and, thus, form a hollow flexible tube with a negatively charged lumen suitable for the transport of DNA. Additionally, we assess the dynamics of the system by combining relaxation measurements with variances in density maps.
フィルム・検出器のモデル: FEI FALCON III (4k x 4k) 検出モード: INTEGRATING / 平均露光時間: 3.0 sec. / 平均電子線量: 70.0 e/Å2 詳細: Images were collected in movie-mode at 20 fractions, each fraction had 6 frames. Dose rate was 23 e/A^2/s, i.e. dose per frame was 3.5 electrons/A^2 .
モデルのタイプ: OTHER 詳細: C6 symmetry and rough estimate of the helical symmetry (rise of 40 A and a twist of 21 deg) was used for initial reconstructions. In the initial model spheres were placed at positions given by the symmetry.
最終 角度割当
タイプ: NOT APPLICABLE
FSC曲線 (解像度の算出)
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原子モデル構築 1
精密化
空間: REAL / プロトコル: AB INITIO MODEL
得られたモデル
PDB-6yeg: Hybrid structure of the SPP1 tail tube by solid-state NMR and cryo EM - Final EM Refinement
PDB-6yq5: Hybrid structure of the SPP1 tail tube by solid-state NMR and cryo EM - NMR Ensemble