- EMDB-10093: Structure of the core of the flagellar export apparatus from Vibr... -
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基本情報
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データベース: EMDB / ID: EMD-10093
タイトル
Structure of the core of the flagellar export apparatus from Vibrio mimicus, the FliPQR-FlhB complex.
マップデータ
Structure of the core of the flagellar export apparatus from Vibrio mimicus, the FliPQR-FlhB complex.
試料
複合体: FliPQR-FlhB
タンパク質・ペプチド: Flagellar biosynthetic protein FliP
タンパク質・ペプチド: Flagellar biosynthetic protein FliR
タンパク質・ペプチド: Flagellar biosynthetic protein FliQ
タンパク質・ペプチド: Flagellar biosynthetic protein FlhB
キーワード
flagella / T3SS / export apparatus / export gate / PROTEIN TRANSPORT
機能・相同性
機能・相同性情報
bacterial-type flagellum organization / bacterial-type flagellum basal body / bacterial-type flagellum assembly / protein secretion / protein targeting / membrane => GO:0016020 / plasma membrane 類似検索 - 分子機能
Flagellar biosynthetic protein FlhB / Flagellar biosynthesis protein FliQ / Flagellar biosynthesis protein FliR / Flagellar transport protein FliP / Type III secretion system substrate exporter / Type III secretion system substrate exporter, C-terminal / FlhB HrpN YscU SpaS Family / Type III secretion system inner membrane R protein / Bacterial export protein family 3 / Bacterial export proteins, family 1 ...Flagellar biosynthetic protein FlhB / Flagellar biosynthesis protein FliQ / Flagellar biosynthesis protein FliR / Flagellar transport protein FliP / Type III secretion system substrate exporter / Type III secretion system substrate exporter, C-terminal / FlhB HrpN YscU SpaS Family / Type III secretion system inner membrane R protein / Bacterial export protein family 3 / Bacterial export proteins, family 1 / Bacterial export proteins, family 3 / Flagella transport protein fliP family signature 1. / Type III secretion system inner membrane P protein / FliP family / Flagella transport protein fliP family signature 2. 類似検索 - ドメイン・相同性
Flagellar biosynthetic protein FliQ / Flagellar biosynthetic protein FlhB / Flagellar biosynthetic protein FliR / Flagellar biosynthetic protein FliP 類似検索 - 構成要素
ジャーナル: Nat Commun / 年: 2020 タイトル: The substrate specificity switch FlhB assembles onto the export gate to regulate type three secretion. 著者: Lucas Kuhlen / Steven Johnson / Andreas Zeitler / Sandra Bäurle / Justin C Deme / Joseph J E Caesar / Rebecca Debo / Joseph Fisher / Samuel Wagner / Susan M Lea / 要旨: Protein secretion through type-three secretion systems (T3SS) is critical for motility and virulence of many bacteria. Proteins are transported through an export gate containing three proteins ...Protein secretion through type-three secretion systems (T3SS) is critical for motility and virulence of many bacteria. Proteins are transported through an export gate containing three proteins (FliPQR in flagella, SctRST in virulence systems). A fourth essential T3SS protein (FlhB/SctU) functions to "switch" secretion substrate specificity once the growing hook/needle reach their determined length. Here, we present the cryo-electron microscopy structure of an export gate containing the switch protein from a Vibrio flagellar system at 3.2 Å resolution. The structure reveals that FlhB/SctU extends the helical export gate with its four predicted transmembrane helices wrapped around FliPQR/SctRST. The unusual topology of the FlhB/SctU helices creates a loop wrapped around the bottom of the closed export gate. Structure-informed mutagenesis suggests that this loop is critical in gating secretion and we propose that a series of conformational changes in the T3SS trigger opening of the gate through interactions between FlhB/SctU and FliPQR/SctRST.