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- EMDB-0834: Structure of the peripheral FCPI from diatom -

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Basic information

Entry
Database: EMDB / ID: EMD-0834
TitleStructure of the peripheral FCPI from diatom
Map data
Sample
  • Complex: peripheral FCPI
    • Protein or peptide: x 10 types
  • Ligand: x 8 types
Biological speciesChaetoceros gracilis (Diatom)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.6 Å
AuthorsNagao R / Kato K / Miyazaki N / Akita F / Shen JR
CitationJournal: Nat Commun / Year: 2020
Title: Structural basis for assembly and function of a diatom photosystem I-light-harvesting supercomplex.
Authors: Ryo Nagao / Koji Kato / Kentaro Ifuku / Takehiro Suzuki / Minoru Kumazawa / Ikuo Uchiyama / Yasuhiro Kashino / Naoshi Dohmae / Seiji Akimoto / Jian-Ren Shen / Naoyuki Miyazaki / Fusamichi Akita /
Abstract: Photosynthetic light-harvesting complexes (LHCs) play a pivotal role in collecting solar energy for photochemical reactions in photosynthesis. One of the major LHCs are fucoxanthin chlorophyll a/c- ...Photosynthetic light-harvesting complexes (LHCs) play a pivotal role in collecting solar energy for photochemical reactions in photosynthesis. One of the major LHCs are fucoxanthin chlorophyll a/c-binding proteins (FCPs) present in diatoms, a group of organisms having important contribution to the global carbon cycle. Here, we report a 2.40-Å resolution structure of the diatom photosystem I (PSI)-FCPI supercomplex by cryo-electron microscopy. The supercomplex is composed of 16 different FCPI subunits surrounding a monomeric PSI core. Each FCPI subunit showed different protein structures with different pigment contents and binding sites, and they form a complicated pigment-protein network together with the PSI core to harvest and transfer the light energy efficiently. In addition, two unique, previously unidentified subunits were found in the PSI core. The structure provides numerous insights into not only the light-harvesting strategy in diatom PSI-FCPI but also evolutionary dynamics of light harvesters among oxyphototrophs.
History
DepositionOct 21, 2019-
Header (metadata) releaseMay 20, 2020-
Map releaseMay 20, 2020-
UpdateMay 20, 2020-
Current statusMay 20, 2020Processing site: PDBj / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.045
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 0.045
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6l4t
  • Surface level: 0.045
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_0834.png

Downloads & links

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Map

FileDownload / File: emd_0834.map.gz / Format: CCP4 / Size: 488.4 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.11 Å/pix.
x 504 pix.
= 560.952 Å		1.11 Å/pix.
x 504 pix.
= 560.952 Å		1.11 Å/pix.
x 504 pix.
= 560.952 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.113 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.045 / Movie #1: 0.045
Minimum - Maximum-0.13163953 - 0.32183647
Average (Standard dev.)0.00013828345 (±0.0032936658)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions504504504
Spacing504504504
CellA=B=C: 560.952 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.1131.1131.113
M x/y/z504504504
origin x/y/z0.0000.0000.000
length x/y/z560.952560.952560.952
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS504504504
D min/max/mean-0.1320.3220.000

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Supplemental data

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Sample components

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Entire : peripheral FCPI

EntireName: peripheral FCPI
Components
  • Complex: peripheral FCPI
    • Protein or peptide: Fucoxanthin chlorophyll a/c-binding protein Lhcr12
    • Protein or peptide: Fucoxanthin chlorophyll a/c-binding protein Lhcr10
    • Protein or peptide: Fucoxanthin chlorophyll a/c-binding protein Lhcr4
    • Protein or peptide: Fucoxanthin chlorophyll a/c-binding protein Lhcr3
    • Protein or peptide: Fucoxanthin chlorophyll a/c-binding protein Lhcq13
    • Protein or peptide: Fucoxanthin chlorophyll a/c-binding protein Lhcq3
    • Protein or peptide: Fucoxanthin chlorophyll a/c-binding protein Lhcq11
    • Protein or peptide: Fucoxanthin chlorophyll a/c-binding protein Lhcq10
    • Protein or peptide: Fucoxanthin chlorophyll a/c-binding protein Lhcq8
    • Protein or peptide: Fucoxanthin chlorophyll a/c-binding protein Lhcq5
  • Ligand: CHLOROPHYLL A
  • Ligand: Chlorophyll c1
  • Ligand: (3S,3'R,5R,6S,7cis)-7',8'-didehydro-5,6-dihydro-5,6-epoxy-beta,beta-carotene-3,3'-diol
  • Ligand: (3S,3'S,5R,5'R,6S,6'R,8'R)-3,5'-dihydroxy-8-oxo-6',7'-didehydro-5,5',6,6',7,8-hexahydro-5,6-epoxy-beta,beta-caroten-3'-yl acetate
  • Ligand: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE
  • Ligand: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE
  • Ligand: DODECYL-BETA-D-MALTOSIDE
  • Ligand: water

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Supramolecule #1: peripheral FCPI

SupramoleculeName: peripheral FCPI / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#10
Source (natural)Organism: Chaetoceros gracilis (Diatom)
Molecular weightTheoretical: 170 KDa

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Macromolecule #1: Fucoxanthin chlorophyll a/c-binding protein Lhcr12

MacromoleculeName: Fucoxanthin chlorophyll a/c-binding protein Lhcr12 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetoceros gracilis (Diatom)
Molecular weightTheoretical: 22.362646 KDa
SequenceString: MKLALLASLI ASAAAFAPSS STGAARASTA LDAGKKSQAL PFLPYPENLA GYVGDAGFDP FRFSDFAPMD FLREAEIKHG RICMLAWLG FVAVDLGARI YPLPEAYEGL TAVTAHDALV QQGAMSQIFL WCSVFEAIST VSVIQMLYEE SGREPGYFGF D PLGFLNGK ...String:
MKLALLASLI ASAAAFAPSS STGAARASTA LDAGKKSQAL PFLPYPENLA GYVGDAGFDP FRFSDFAPMD FLREAEIKHG RICMLAWLG FVAVDLGARI YPLPEAYEGL TAVTAHDALV QQGAMSQIFL WCSVFEAIST VSVIQMLYEE SGREPGYFGF D PLGFLNGK SEAEVNEMKL KEIKNGRLAM LAFSGVVTQA VLTQGPFPYV

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Macromolecule #2: Fucoxanthin chlorophyll a/c-binding protein Lhcr10

MacromoleculeName: Fucoxanthin chlorophyll a/c-binding protein Lhcr10 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetoceros gracilis (Diatom)
Molecular weightTheoretical: 32.238152 KDa
SequenceString: LLQGFSRWLV FFVSIRIIRH PCRPPYHETY SSSNTFLSIQ YIEQSHSARQ TSLSLLLITP LTLVLYLAIY TILKMFKTAA AITSLLAAS ASAFAPSSFN GRISTAVAAE KSQSLPFMNR PPLLDGSMAG DVGFDPLGLS NIDDVGIDLY WLREAEIKHC R VAMLAVVG ...String:
LLQGFSRWLV FFVSIRIIRH PCRPPYHETY SSSNTFLSIQ YIEQSHSARQ TSLSLLLITP LTLVLYLAIY TILKMFKTAA AITSLLAAS ASAFAPSSFN GRISTAVAAE KSQSLPFMNR PPLLDGSMAG DVGFDPLGLS NIDDVGIDLY WLREAEIKHC R VAMLAVVG ILQVEIFGPA PGCEMATDKC QMDAFWQIWG AHPQYIAFGL IMIMMIEMIS GIATTQGRES GERAPGDFGL DP LGYGKGD AAGYARLQAQ EIANGRLAMF AAAGEIMQGC TTHQGALENL MTALRDNSF

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Macromolecule #3: Fucoxanthin chlorophyll a/c-binding protein Lhcr4

MacromoleculeName: Fucoxanthin chlorophyll a/c-binding protein Lhcr4 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetoceros gracilis (Diatom)
Molecular weightTheoretical: 29.70415 KDa
SequenceString: LRSIHHLIAS HRRLFKFIRH YTINKMKSAL IATALLAGSA AAFTSNAGSQ STSALKAMPE RLWDSMVDKT ERSKAVPFLP RAVNLDGSL PGDVGFDPFY LSSIPKDFSG FIQPPQWEEK GIPTLYWMRE AELKHCRVAM LAWFGWLATD GAFGVTLRFP G EIYSVENI ...String:
LRSIHHLIAS HRRLFKFIRH YTINKMKSAL IATALLAGSA AAFTSNAGSQ STSALKAMPE RLWDSMVDKT ERSKAVPFLP RAVNLDGSL PGDVGFDPFY LSSIPKDFSG FIQPPQWEEK GIPTLYWMRE AELKHCRVAM LAWFGWLATD GAFGVTLRFP G EIYSVENI PTAYEAHNAL VSQGSMGFLL LAVGFIEFCT GAVLVEVAKG ASDREAGDFK LDPLSFLKGK SEEEIKRMKT RE IANGRLA MLAFGGVATQ TALEGGNHAF PYF

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Macromolecule #4: Fucoxanthin chlorophyll a/c-binding protein Lhcr3

MacromoleculeName: Fucoxanthin chlorophyll a/c-binding protein Lhcr3 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetoceros gracilis (Diatom)
Molecular weightTheoretical: 22.216549 KDa
SequenceString: MKSVAILAAL FGSATAFVPS QVSRTAASTS VKASLADMVG AEGPEPIPFA PSKTSKNFDP VGFAERSPEW LPWYREAELK HGRAAMLAT VGFVVPEFIR VPGEQFSFEA IPKVIDAHDA LPESMIQIFG WISFLEACTF PAMAGLGGKY DRKPGDFSFD P LGLYPTDP ...String:
MKSVAILAAL FGSATAFVPS QVSRTAASTS VKASLADMVG AEGPEPIPFA PSKTSKNFDP VGFAERSPEW LPWYREAELK HGRAAMLAT VGFVVPEFIR VPGEQFSFEA IPKVIDAHDA LPESMIQIFG WISFLEACTF PAMAGLGGKY DRKPGDFSFD P LGLYPTDP EKQKQMQLAE LKNGRLAMIA IGGMVTGAAV TGHGFPYLP

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Macromolecule #5: Fucoxanthin chlorophyll a/c-binding protein Lhcq13

MacromoleculeName: Fucoxanthin chlorophyll a/c-binding protein Lhcq13 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetoceros gracilis (Diatom)
Molecular weightTheoretical: 24.842496 KDa
SequenceString: MKTAAIFALL AGSAAAFAPT TVSPRASTVA HMSSINEAFG ISIETGNKCP PLGARILEDA QPSAIKWFQN AEIKHGRIAM VATIGYLVQ KLGVHFPLYL GPSGSNCFHP ESETAWLLSS STGVTFSDIA KAAPLDAIQM VPVAGWMQIF FVAGWFESVA Y YRQWVKNS ...String:
MKTAAIFALL AGSAAAFAPT TVSPRASTVA HMSSINEAFG ISIETGNKCP PLGARILEDA QPSAIKWFQN AEIKHGRIAM VATIGYLVQ KLGVHFPLYL GPSGSNCFHP ESETAWLLSS STGVTFSDIA KAAPLDAIQM VPVAGWMQIF FVAGWFESVA Y YRQWVKNS PIPGDYGYDP LGFTKREGGW DSEELTSLRL KEIKNGRLAM MTIAAWVSDE MIPGALPVWH P

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Macromolecule #6: Fucoxanthin chlorophyll a/c-binding protein Lhcq3

MacromoleculeName: Fucoxanthin chlorophyll a/c-binding protein Lhcq3 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetoceros gracilis (Diatom)
Molecular weightTheoretical: 21.318404 KDa
SequenceString: MKSVLFLALA GSAAAFAPST QSRSNTLLKA DLSSLPGSTA PVGPFDPLNL ADSGSEETLA WFRASELKHG RVAMLATTGY LVQGAGIHF PGMLSSDVSF ESLSAMKPLE AWDAVPDAGK AQILGTIFIA EMITESKPVH YTKGGPMPTM VFPAIDFSGV D AATLKRKQ ...String:
MKSVLFLALA GSAAAFAPST QSRSNTLLKA DLSSLPGSTA PVGPFDPLNL ADSGSEETLA WFRASELKHG RVAMLATTGY LVQGAGIHF PGMLSSDVSF ESLSAMKPLE AWDAVPDAGK AQILGTIFIA EMITESKPVH YTKGGPMPTM VFPAIDFSGV D AATLKRKQ DSELNNGRLA MIAIMSFISA ANIPGSVPAL TNNPAF

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Macromolecule #7: Fucoxanthin chlorophyll a/c-binding protein Lhcq11

MacromoleculeName: Fucoxanthin chlorophyll a/c-binding protein Lhcq11 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetoceros gracilis (Diatom)
Molecular weightTheoretical: 25.994562 KDa
SequenceString: MKLAILTTLL AGASAFTTPN ARPAFATKLS MSEEAAAEEA PAADAPEEVV EPASPSYTCI SKEAILSSPD TTEIGRVWDP LGLAEIGSA ETLAWYRHSE VKHGRIAMAA FVGWWAVGAG LRFPGELSHG LEFSSIPSKG LEAWDAVPGW GKAQMLLFAG L IEFHDELF ...String:
MKLAILTTLL AGASAFTTPN ARPAFATKLS MSEEAAAEEA PAADAPEEVV EPASPSYTCI SKEAILSSPD TTEIGRVWDP LGLAEIGSA ETLAWYRHSE VKHGRIAMAA FVGWWAVGAG LRFPGELSHG LEFSSIPSKG LEAWDAVPGW GKAQMLLFAG L IEFHDELF HTRRTEGGHY LRGGTPGKNM VPGLFDPFGF SKGKSEEELA KGRDREIKNG RLAMIGVAGL YCAATIPGSV PL QPPC

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Macromolecule #8: Fucoxanthin chlorophyll a/c-binding protein Lhcq10

MacromoleculeName: Fucoxanthin chlorophyll a/c-binding protein Lhcq10 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetoceros gracilis (Diatom)
Molecular weightTheoretical: 26.875781 KDa
SequenceString: MKIILSGLAL LASSVAAFAP QSIISANANN KNANVVLEAA KDDLIAIAEK SNPVLKYYDP LQLGSTTIWG ETNSATIGFL RQSEIKHGR IAMAAFVGYI VQANGIHFPW PMSFDGTPFP ADAGSPPEQW DALSDAAKWQ IILFIGFLEW FSEAAGKHYM R GGKPGAFP ...String:
MKIILSGLAL LASSVAAFAP QSIISANANN KNANVVLEAA KDDLIAIAEK SNPVLKYYDP LQLGSTTIWG ETNSATIGFL RQSEIKHGR IAMAAFVGYI VQANGIHFPW PMSFDGTPFP ADAGSPPEQW DALSDAAKWQ IILFIGFLEW FSEAAGKHYM R GGKPGAFP NFSDSDLIPH PVPLNLYDPF GFSKGKTEAQ KADGLIKELN NGRLAMIGIM GFLAEQKVEG SVPLLKGVVP HY DGEVMAP FM

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Macromolecule #9: Fucoxanthin chlorophyll a/c-binding protein Lhcq8

MacromoleculeName: Fucoxanthin chlorophyll a/c-binding protein Lhcq8 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetoceros gracilis (Diatom)
Molecular weightTheoretical: 30.545674 KDa
SequenceString: MDNKIAGYNI IIHTIISSIN MKFSLVVLSS LVVASSNGVT AFAPSSSTTS TSTTALSSSA SKAELEAIAK KANPTLGYYD PLSLADKDF WGKGNDATIA FLRQSEIKHG RIAMFAFVGY IVQSNFVFPW AQTLAGAPHP SADLSPEAQW DAIPLGAKWQ I FAVISALE ...String:
MDNKIAGYNI IIHTIISSIN MKFSLVVLSS LVVASSNGVT AFAPSSSTTS TSTTALSSSA SKAELEAIAK KANPTLGYYD PLSLADKDF WGKGNDATIA FLRQSEIKHG RIAMFAFVGY IVQSNFVFPW AQTLAGAPHP SADLSPEAQW DAIPLGAKWQ I FAVISALE LWDECGGGGV LPHYTKGRKP GQYPPFTLFR DNVHFVLDLY DPFGFNKNMS EETKERRLVS ELNNGRLAML GI FGFLCAD TIPGSVPLLN DIAIPYSGQV MQPFEGQFSY FGSL

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Macromolecule #10: Fucoxanthin chlorophyll a/c-binding protein Lhcq5

MacromoleculeName: Fucoxanthin chlorophyll a/c-binding protein Lhcq5 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetoceros gracilis (Diatom)
Molecular weightTheoretical: 23.533191 KDa
SequenceString: MKIATLFLAL ASSAAAFAPS QQVRSMTNEK MKPRQARNNF ALNMKVDEMP GATAPLGKFD PLNLATLGSE STLAWFRAAE LKHSRVAML ATTGYLVQAA GIHFPGMLSS DVSFESLSAM KPLDAWDAVP EGGKNQIYFT IFLAEFITEC KGTHYTKGGP L PTIVFPPI ...String:
MKIATLFLAL ASSAAAFAPS QQVRSMTNEK MKPRQARNNF ALNMKVDEMP GATAPLGKFD PLNLATLGSE STLAWFRAAE LKHSRVAML ATTGYLVQAA GIHFPGMLSS DVSFESLSAM KPLDAWDAVP EGGKNQIYFT IFLAEFITEC KGTHYTKGGP L PTIVFPPI DFSTVNPEQL KTRQNRELNN GRLAMIAIMS FVAAANIPGS VPALAGNPMF

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Macromolecule #11: CHLOROPHYLL A

MacromoleculeName: CHLOROPHYLL A / type: ligand / ID: 11 / Number of copies: 84 / Formula: CLA
Molecular weightTheoretical: 893.489 Da
Chemical component information

ChemComp-CLA:
CHLOROPHYLL A

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Macromolecule #12: Chlorophyll c1

MacromoleculeName: Chlorophyll c1 / type: ligand / ID: 12 / Number of copies: 35 / Formula: KC1
Molecular weightTheoretical: 610.941 Da
Chemical component information

ChemComp-KC1:
Chlorophyll c1

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Macromolecule #13: (3S,3'R,5R,6S,7cis)-7',8'-didehydro-5,6-dihydro-5,6-epoxy-beta,be...

MacromoleculeName: (3S,3'R,5R,6S,7cis)-7',8'-didehydro-5,6-dihydro-5,6-epoxy-beta,beta-carotene-3,3'-diol
type: ligand / ID: 13 / Number of copies: 18 / Formula: DD6
Molecular weightTheoretical: 582.855 Da
Chemical component information

ChemComp-DD6:
(3S,3'R,5R,6S,7cis)-7',8'-didehydro-5,6-dihydro-5,6-epoxy-beta,beta-carotene-3,3'-diol

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Macromolecule #14: (3S,3'S,5R,5'R,6S,6'R,8'R)-3,5'-dihydroxy-8-oxo-6',7'-didehydro-5...

MacromoleculeName: (3S,3'S,5R,5'R,6S,6'R,8'R)-3,5'-dihydroxy-8-oxo-6',7'-didehydro-5,5',6,6',7,8-hexahydro-5,6-epoxy-beta,beta-caroten-3'-yl acetate
type: ligand / ID: 14 / Number of copies: 36 / Formula: A86
Molecular weightTheoretical: 658.906 Da

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Macromolecule #15: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE

MacromoleculeName: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / type: ligand / ID: 15 / Number of copies: 1 / Formula: LHG
Molecular weightTheoretical: 722.97 Da
Chemical component information

ChemComp-LHG:
1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM

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Macromolecule #16: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

MacromoleculeName: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE / type: ligand / ID: 16 / Number of copies: 5 / Formula: LMG
Molecular weightTheoretical: 787.158 Da
Chemical component information

ChemComp-LMG:
1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

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Macromolecule #17: DODECYL-BETA-D-MALTOSIDE

MacromoleculeName: DODECYL-BETA-D-MALTOSIDE / type: ligand / ID: 17 / Number of copies: 13 / Formula: LMT
Molecular weightTheoretical: 510.615 Da
Chemical component information

ChemComp-LMT:
DODECYL-BETA-D-MALTOSIDE / detergent*YM

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Macromolecule #18: water

MacromoleculeName: water / type: ligand / ID: 18 / Number of copies: 12 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration2 mg/mL
BufferpH: 6.5
Component:
ConcentrationFormulaName
20.0 mMMES-NaOHMES-NaOH
0.02 %DDMDDM
GridModel: C-flat-1.2/1.3 / Material: MOLYBDENUM / Mesh: 300 / Support film - Material: CARBON / Pretreatment - Type: PLASMA CLEANING
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: FEI FALCON III (4k x 4k) / Detector mode: COUNTING / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 2689965
CTF correctionSoftware - Name: CTFFIND (ver. 4)
Startup modelType of model: EMDB MAP
EMDB ID:

9853

Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 2.6 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3) / Number images used: 470801
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3)
FSC plot (resolution estimation)

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Atomic model buiding 1

RefinementSpace: REAL / Protocol: FLEXIBLE FIT
Output model

PDB-6l4t:
Structure of the peripheral FCPI from diatom

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