登録情報 データベース : EMDB / ID : EMD-0559 構造の表示 ダウンロードとリンクタイトル Set2 bound to nucleosome マップデータSet2 bound to the nucleosome 詳細 試料複合体 : Set2 nucleosome complex複合体 : Histone-lysine N-methyltransferaseタンパク質・ペプチド : Histone-lysine N-methyltransferase複合体 : DNADNA : DNA (149-MER)DNA : DNA (149-MER)複合体 : Histone H3, Histone H4, Ubiquitin-60S ribosomal protein L40,Histone H2A, Histone H2B 1.1タンパク質・ペプチド : Histone H3タンパク質・ペプチド : Histone H4タンパク質・ペプチド : Ubiquitin-60S ribosomal protein L40,Histone H2Aタンパク質・ペプチド : Histone H2B 1.1リガンド : ZINC ION 詳細機能・相同性 機能・相同性情報分子機能 ドメイン・相同性 構成要素
: / [histone H3]-lysine36 N-trimethyltransferase / histone H3K36 methyltransferase activity / ribosomal large subunit export from nucleus / ribosomal large subunit assembly / modification-dependent protein catabolic process / structural constituent of chromatin / protein tag activity / nucleosome / nucleosome assembly ... : / [histone H3]-lysine36 N-trimethyltransferase / histone H3K36 methyltransferase activity / ribosomal large subunit export from nucleus / ribosomal large subunit assembly / modification-dependent protein catabolic process / structural constituent of chromatin / protein tag activity / nucleosome / nucleosome assembly / ribosome biogenesis / chromosome / cytosolic large ribosomal subunit / cytoplasmic translation / protein ubiquitination / structural constituent of ribosome / protein heterodimerization activity / ubiquitin protein ligase binding / regulation of DNA-templated transcription / mitochondrion / DNA binding / nucleus / cytosol 類似検索 - 分子機能 Histone-lysine N-methyltransferase, Set2, fungi / Histone-lysine N-methyltransferase (EC 2.1.1.43) family profile. / Set2 Rpb1 interacting domain / Set2 Rpb1 interacting domain superfamily / SRI (Set2 Rpb1 interacting) domain / SETD2/Set2, SET domain / AWS domain / AWS domain / AWS domain profile. / associated with SET domains ... Histone-lysine N-methyltransferase, Set2, fungi / Histone-lysine N-methyltransferase (EC 2.1.1.43) family profile. / Set2 Rpb1 interacting domain / Set2 Rpb1 interacting domain superfamily / SRI (Set2 Rpb1 interacting) domain / SETD2/Set2, SET domain / AWS domain / AWS domain / AWS domain profile. / associated with SET domains / TFIIS/LEDGF domain superfamily / Cysteine-rich motif following a subset of SET domains / Post-SET domain / Post-SET domain profile. / WW domain superfamily / SET (Su(var)3-9, Enhancer-of-zeste, Trithorax) domain / WW/rsp5/WWP domain profile. / Domain with 2 conserved Trp (W) residues / SET domain superfamily / WW domain / SET domain / SET domain profile. / SET domain / Ribosomal L40e family / Ribosomal_L40e / Ribosomal protein L40e / Ribosomal protein L40e superfamily / Histone H2B signature. / Histone H2B / Histone H2B / Histone H2A conserved site / Histone H2A signature. / Histone H2A, C-terminal domain / C-terminus of histone H2A / Histone H2A / Histone 2A / Histone H4, conserved site / Histone H4 signature. / Histone H4 / Histone H4 / TATA box binding protein associated factor / TATA box binding protein associated factor (TAF), histone-like fold domain / CENP-T/Histone H4, histone fold / Centromere kinetochore component CENP-T histone fold / Histone H3 signature 1. / Histone H3 signature 2. / Histone H3 / Histone H3/CENP-A / Histone H2A/H2B/H3 / Core histone H2A/H2B/H3/H4 / Ubiquitin domain signature. / Ubiquitin conserved site / Ubiquitin domain / Histone-fold / Ubiquitin family / Ubiquitin homologues / Ubiquitin domain profile. / Ubiquitin-like domain / Ubiquitin-like domain superfamily 類似検索 - ドメイン・相同性 Histone H3 / Histone-lysine N-methyltransferase, H3 lysine-36 specific / Histone H2B 1.1 / Histone H3.3C / Ubiquitin-ribosomal protein eL40B fusion protein / Histone H4 / Histone H2A 類似検索 - 構成要素生物種 Chaetomium thermophilum (菌類) / synthetic construct (人工物) / Xenopus laevis (アフリカツメガエル) / Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (菌類)手法 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度 : 3.8 Å 詳細 データ登録者Halic M / Bilokapic S 引用ジャーナル : Nat Commun / 年 : 2019タイトル : Nucleosome and ubiquitin position Set2 to methylate H3K36.著者 : Silvija Bilokapic / Mario Halic / 要旨 : Histone H3 lysine 36 methylation (H3K36me) is a conserved histone modification deposited by the Set2 methyltransferases. Recent findings show that over-expression or mutation of Set2 enzymes promotes ... Histone H3 lysine 36 methylation (H3K36me) is a conserved histone modification deposited by the Set2 methyltransferases. Recent findings show that over-expression or mutation of Set2 enzymes promotes cancer progression, however, mechanisms of H3K36me are poorly understood. Set2 enzymes show spurious activity on histones and histone tails, and it is unknown how they obtain specificity to methylate H3K36 on the nucleosome. In this study, we present 3.8 Å cryo-EM structure of Set2 bound to the mimic of H2B ubiquitinated nucleosome. Our structure shows that Set2 makes extensive interactions with the H3 αN, the H3 tail, the H2A C-terminal tail and stabilizes DNA in the unwrapped conformation, which positions Set2 to specifically methylate H3K36. Moreover, we show that ubiquitin contributes to Set2 positioning on the nucleosome and stimulates the methyltransferase activity. Notably, our structure uncovers interfaces that can be targeted by small molecules for development of future cancer therapies. 履歴 登録 2019年2月14日 - ヘッダ(付随情報) 公開 2019年7月17日 - マップ公開 2019年8月28日 - 更新 2019年9月4日 - 現状 2019年9月4日 処理サイト : RCSB / 状態 : 公開
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