+データを開く
-基本情報
登録情報 | データベース: EMDB / ID: EMD-4358 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
タイトル | Coupling specificity of heterotrimeric Go to the serotonin 5-HT1B receptor | |||||||||
マップデータ | Map after RELION "postprocessing" sharpened with a B=-200 and weighted by FSC. | |||||||||
試料 |
| |||||||||
機能・相同性 | 機能・相同性情報 guanyl nucleotide binding / voltage-gated calcium channel activity involved in regulation of presynaptic cytosolic calcium levels / negative regulation of gamma-aminobutyric acid secretion / serotonergic synapse / G protein-coupled serotonin receptor complex / adenylate cyclase-inhibiting serotonin receptor signaling pathway / dopamine neurotransmitter receptor activity, coupled via Gs / regulation of behavior / negative regulation of synaptic transmission, GABAergic / response to mineralocorticoid ...guanyl nucleotide binding / voltage-gated calcium channel activity involved in regulation of presynaptic cytosolic calcium levels / negative regulation of gamma-aminobutyric acid secretion / serotonergic synapse / G protein-coupled serotonin receptor complex / adenylate cyclase-inhibiting serotonin receptor signaling pathway / dopamine neurotransmitter receptor activity, coupled via Gs / regulation of behavior / negative regulation of synaptic transmission, GABAergic / response to mineralocorticoid / Serotonin receptors / negative regulation of serotonin secretion / drinking behavior / cellular response to temperature stimulus / bone remodeling / serotonin binding / negative regulation of synaptic transmission, glutamatergic / vasoconstriction / G protein-coupled serotonin receptor activity / : / G protein-coupled receptor internalization / vesicle docking involved in exocytosis / neurotransmitter receptor activity / G protein-coupled dopamine receptor signaling pathway / regulation of heart contraction / regulation of synaptic vesicle exocytosis / cellular response to alkaloid / regulation of dopamine secretion / calyx of Held / G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger / mu-type opioid receptor binding / corticotropin-releasing hormone receptor 1 binding / negative regulation of insulin secretion / G protein-coupled serotonin receptor binding / adenylate cyclase-activating adrenergic receptor signaling pathway / positive regulation of vascular associated smooth muscle cell proliferation / presynaptic modulation of chemical synaptic transmission / adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway / response to cocaine / locomotory behavior / muscle contraction / G-protein beta/gamma-subunit complex binding / Olfactory Signaling Pathway / Activation of the phototransduction cascade / adenylate cyclase-modulating G protein-coupled receptor signaling pathway / G beta:gamma signalling through PLC beta / Presynaptic function of Kainate receptors / Thromboxane signalling through TP receptor / G-protein activation / G protein-coupled acetylcholine receptor signaling pathway / Activation of G protein gated Potassium channels / Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits / Prostacyclin signalling through prostacyclin receptor / Glucagon signaling in metabolic regulation / G beta:gamma signalling through CDC42 / ADP signalling through P2Y purinoceptor 12 / G beta:gamma signalling through BTK / Synthesis, secretion, and inactivation of Glucagon-like Peptide-1 (GLP-1) / Sensory perception of sweet, bitter, and umami (glutamate) taste / photoreceptor disc membrane / Adrenaline,noradrenaline inhibits insulin secretion / Glucagon-type ligand receptors / Vasopressin regulates renal water homeostasis via Aquaporins / G alpha (z) signalling events / cellular response to catecholamine stimulus / Glucagon-like Peptide-1 (GLP1) regulates insulin secretion / ADORA2B mediated anti-inflammatory cytokines production / sensory perception of taste / ADP signalling through P2Y purinoceptor 1 / adenylate cyclase-activating dopamine receptor signaling pathway / G beta:gamma signalling through PI3Kgamma / cellular response to prostaglandin E stimulus / Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding / GPER1 signaling / G-protein beta-subunit binding / Inactivation, recovery and regulation of the phototransduction cascade / heterotrimeric G-protein complex / G alpha (12/13) signalling events / extracellular vesicle / cellular response to xenobiotic stimulus / signaling receptor complex adaptor activity / Thrombin signalling through proteinase activated receptors (PARs) / GTPase binding / retina development in camera-type eye / presynaptic membrane / phospholipase C-activating G protein-coupled receptor signaling pathway / Ca2+ pathway / cell body / G alpha (i) signalling events / fibroblast proliferation / G alpha (s) signalling events / G alpha (q) signalling events / chemical synaptic transmission / response to ethanol / cell population proliferation / Ras protein signal transduction / Extra-nuclear estrogen signaling / G protein-coupled receptor signaling pathway / lysosomal membrane / GTPase activity 類似検索 - 分子機能 | |||||||||
生物種 | Homo sapiens (ヒト) | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.78 Å | |||||||||
データ登録者 | Garcia-Nafria J / Nehme R / Edwards P / Tate CG | |||||||||
資金援助 | 英国, 2件
| |||||||||
引用 | ジャーナル: Nature / 年: 2018 タイトル: Cryo-EM structure of the serotonin 5-HT receptor coupled to heterotrimeric G. 著者: Javier García-Nafría / Rony Nehmé / Patricia C Edwards / Christopher G Tate / 要旨: G-protein-coupled receptors (GPCRs) form the largest family of receptors encoded by the human genome (around 800 genes). They transduce signals by coupling to a small number of heterotrimeric G ...G-protein-coupled receptors (GPCRs) form the largest family of receptors encoded by the human genome (around 800 genes). They transduce signals by coupling to a small number of heterotrimeric G proteins (16 genes encoding different α-subunits). Each human cell contains several GPCRs and G proteins. The structural determinants of coupling of G to four different GPCRs have been elucidated, but the molecular details of how the other G-protein classes couple to GPCRs are unknown. Here we present the cryo-electron microscopy structure of the serotonin 5-HT receptor (5-HTR) bound to the agonist donitriptan and coupled to an engineered G heterotrimer. In this complex, 5-HTR is in an active state; the intracellular domain of the receptor is in a similar conformation to that observed for the β-adrenoceptor (βAR) or the adenosine A receptor (AR) in complex with G. In contrast to the complexes with G, the gap between the receptor and the Gβ-subunit in the G-5-HTR complex precludes molecular contacts, and the interface between the Gα-subunit of G and the receptor is considerably smaller. These differences are likely to be caused by the differences in the interactions with the C terminus of the G α-subunit. The molecular variations between the interfaces of G and G in complex with GPCRs may contribute substantially to both the specificity of coupling and the kinetics of signalling. | |||||||||
履歴 |
|
-構造の表示
ムービー |
ムービービューア |
---|---|
構造ビューア | EMマップ: SurfViewMolmilJmol/JSmol |
添付画像 |
-ダウンロードとリンク
-EMDBアーカイブ
マップデータ | emd_4358.map.gz | 5.3 MB | EMDBマップデータ形式 | |
---|---|---|---|---|
ヘッダ (付随情報) | emd-4358-v30.xml emd-4358.xml | 20 KB 20 KB | 表示 表示 | EMDBヘッダ |
FSC (解像度算出) | emd_4358_fsc.xml | 6.3 KB | 表示 | FSCデータファイル |
画像 | emd_4358.png | 125.3 KB | ||
アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-4358 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-4358 | HTTPS FTP |
-検証レポート
文書・要旨 | emd_4358_validation.pdf.gz | 273.4 KB | 表示 | EMDB検証レポート |
---|---|---|---|---|
文書・詳細版 | emd_4358_full_validation.pdf.gz | 272.5 KB | 表示 | |
XML形式データ | emd_4358_validation.xml.gz | 8.5 KB | 表示 | |
アーカイブディレクトリ | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-4358 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-4358 | HTTPS FTP |
-関連構造データ
関連構造データ | 6g79MC M: このマップから作成された原子モデル C: 同じ文献を引用 (文献) |
---|---|
類似構造データ | |
電子顕微鏡画像生データ | EMPIAR-10308 (タイトル: Cryo-EM structure of the serotonin 5-HT1B receptor coupled to heterotrimeric Go Data size: 8.0 TB / Data #1: Unaligned movies [micrographs - multiframe] Data #2: Extracted particles [picked particles - multiframe - processed]) |
-リンク
EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
---|---|
「今月の分子」の関連する項目 |
-マップ
ファイル | ダウンロード / ファイル: emd_4358.map.gz / 形式: CCP4 / 大きさ: 12.9 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
注釈 | Map after RELION "postprocessing" sharpened with a B=-200 and weighted by FSC. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.06 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
|
-添付データ
-試料の構成要素
+全体 : Serotonin 5-HT1B receptor bound to a mini-Go heterotrimer
+超分子 #1: Serotonin 5-HT1B receptor bound to a mini-Go heterotrimer
+超分子 #2: 5-HT1B receptor
+超分子 #3: Beta subunit
+超分子 #4: mini-Go
+超分子 #5: Gamma subunit
+分子 #1: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
+分子 #2: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2
+分子 #3: Guanine nucleotide-binding protein G(o) subunit alpha
+分子 #4: 5-hydroxytryptamine receptor 1B
+分子 #5: 2-[5-[2-[4-(4-cyanophenyl)piperazin-1-yl]-2-oxidanylidene-ethoxy]...
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
---|---|
解析 | 単粒子再構成法 |
試料の集合状態 | particle |
-試料調製
濃度 | 2.2 mg/mL | ||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
緩衝液 | pH: 7.5 構成要素:
| ||||||||||||||||||
グリッド | モデル: Quantifoil R1.2/1.3 / 材質: GOLD / メッシュ: 300 / 前処理 - タイプ: GLOW DISCHARGE | ||||||||||||||||||
凍結 | 凍結剤: ETHANE / チャンバー内湿度: 100 % / チャンバー内温度: 277.15 K / 装置: FEI VITROBOT MARK IV |
-電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
---|---|
撮影 | フィルム・検出器のモデル: FEI FALCON III (4k x 4k) 検出モード: COUNTING / 実像数: 5737 / 平均露光時間: 60.0 sec. / 平均電子線量: 30.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
電子光学系 | C2レンズ絞り径: 50.0 µm / 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / Cs: 2.7 mm |
試料ステージ | 試料ホルダーモデル: FEI TITAN KRIOS AUTOGRID HOLDER ホルダー冷却材: NITROGEN |
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |