登録情報 データベース : EMDB / ID : EMD-4225 構造の表示 ダウンロードとリンクタイトル Cryo-EM structure of the human CPSF160-WDR33-CPSF30 complex bound to the PAS AAUAAA motif at 3.1 Angstrom resolution. マップデータOutput from Relion2 PostProcess 詳細 試料複合体 : Human CPSF160-WDR33-CPSF30 heterotrimer bound to the AAUAAA motif of the Polyadenylation Signal複合体 : Human CPSF160-WDR33-CPSF30 heterotrimerタンパク質・ペプチド : Cleavage and polyadenylation specificity factor subunit 1タンパク質・ペプチド : pre-mRNA 3' end processing protein WDR33タンパク質・ペプチド : Cleavage and polyadenylation specificity factor subunit 4複合体 : AAUAAA motif of the Polyadenylation SignalRNA : RNA (5'-R(P*AP*AP*UP*AP*AP*AP*GP*G)-3')リガンド : ZINC ION 詳細機能・相同性 機能・相同性情報分子機能 ドメイン・相同性 構成要素
co-transcriptional RNA 3'-end processing, cleavage and polyadenylation pathway / Inhibition of Host mRNA Processing and RNA Silencing / Processing of Intronless Pre-mRNAs / mRNA cleavage and polyadenylation specificity factor complex / collagen trimer / mRNA 3'-UTR AU-rich region binding / Transport of Mature mRNA Derived from an Intronless Transcript / mRNA 3'-end processing / mRNA 3'-end processing / postreplication repair ... co-transcriptional RNA 3'-end processing, cleavage and polyadenylation pathway / Inhibition of Host mRNA Processing and RNA Silencing / Processing of Intronless Pre-mRNAs / mRNA cleavage and polyadenylation specificity factor complex / collagen trimer / mRNA 3'-UTR AU-rich region binding / Transport of Mature mRNA Derived from an Intronless Transcript / mRNA 3'-end processing / mRNA 3'-end processing / postreplication repair / tRNA processing in the nucleus / RNA Polymerase II Transcription Termination / Processing of Capped Intron-Containing Pre-mRNA / fibrillar center / mRNA processing / sequence-specific double-stranded DNA binding / spermatogenesis / intracellular membrane-bounded organelle / enzyme binding / RNA binding / zinc ion binding / nucleoplasm / nucleus 類似検索 - 分子機能 Zinc-finger CCCH domain / Zinc-finger containing family / CPSF complex subunit CPSF4-like / Pre-mRNA 3' end processing protein Pfs2-like / Zinc finger C-x8-C-x5-C-x3-H type (and similar) / Zinc finger, CCCH-type superfamily / zinc finger / Zinc finger, CCCH-type / Collagen triple helix repeat / Collagen triple helix repeat (20 copies) ... Zinc-finger CCCH domain / Zinc-finger containing family / CPSF complex subunit CPSF4-like / Pre-mRNA 3' end processing protein Pfs2-like / Zinc finger C-x8-C-x5-C-x3-H type (and similar) / Zinc finger, CCCH-type superfamily / zinc finger / Zinc finger, CCCH-type / Collagen triple helix repeat / Collagen triple helix repeat (20 copies) / Zinc finger C3H1-type profile. / Cleavage/polyadenylation specificity factor, A subunit, N-terminal / Mono-functional DNA-alkylating methyl methanesulfonate N-term / : / Cleavage/polyadenylation specificity factor, A subunit, C-terminal / CPSF A subunit region / zinc finger / Zinc knuckle / Zinc finger, CCHC-type superfamily / Zinc finger, CCHC-type / Zinc finger CCHC-type profile. / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD domain, G-beta repeat / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily 類似検索 - ドメイン・相同性 Cleavage and polyadenylation specificity factor subunit 4 / Cleavage and polyadenylation specificity factor subunit 1 / pre-mRNA 3' end processing protein WDR33 類似検索 - 構成要素生物種 Homo sapiens (ヒト) / unidentified adenovirus (ウイルス)手法 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度 : 3.07 Å 詳細 データ登録者Clerici M / Faini M / Jinek M 資金援助 ベルギー, ドイツ, 4件 詳細 詳細を隠すOrganization Grant number 国 European Research Council ERC-StG-337284 ベルギー European Molecular Biology Organization EMBO ALTF-343-2013 ドイツ European Research Council ERC Advanced Grants no. 233226 and no. 670821 ベルギー European Union PROSPECTS, HEALTH-F4-2008-201648 ベルギー
引用ジャーナル : Nat Struct Mol Biol / 年 : 2018タイトル : Structural basis of AAUAAA polyadenylation signal recognition by the human CPSF complex.著者 : Marcello Clerici / Marco Faini / Lena M Muckenfuss / Ruedi Aebersold / Martin Jinek / 要旨 : Mammalian mRNA biogenesis requires specific recognition of a hexanucleotide AAUAAA motif in the polyadenylation signals (PAS) of precursor mRNA (pre-mRNA) transcripts by the cleavage and ... Mammalian mRNA biogenesis requires specific recognition of a hexanucleotide AAUAAA motif in the polyadenylation signals (PAS) of precursor mRNA (pre-mRNA) transcripts by the cleavage and polyadenylation specificity factor (CPSF) complex. Here we present a 3.1-Å-resolution cryo-EM structure of a core CPSF module bound to the PAS hexamer motif. The structure reveals the molecular interactions responsible for base-specific recognition, providing a rationale for mechanistic differences between mammalian and yeast 3' polyadenylation. 履歴 登録 2017年12月19日 - ヘッダ(付随情報) 公開 2018年1月3日 - マップ公開 2018年1月31日 - 更新 2023年3月1日 - 現状 2023年3月1日 処理サイト : PDBe / 状態 : 公開
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