EMDB-29255: Human nucleolar pre-60S ribosomal subunit (State B2) - Composite map

Basic information

Entry

Database: EMDB / ID: EMD-29255

• Title: Human nucleolar pre-60S ribosomal subunit (State B2) - Composite map

Sample

• Complex: Human nucleolar pre-60S ribosomal subunit (State B2)
  • Protein or peptide: x 44 types
  • RNA: x 3 types
• Ligand: x 4 types

• Keywords: Pre-60S ribosomal subunit / Assembly intermediate / Ribosome / Nucleoprotein complex

Function / homology

Function:

RNA 2'-O-methyltransferase activity / granular component / negative regulation of collagen binding / rRNA (uridine-2'-O-)-methyltransferase activity / rRNA (guanine) methyltransferase activity / preribosome binding / regulation of cellular senescence / lamin filament / regulation of fatty acid biosynthetic process / regulation of megakaryocyte differentiation / RNA methylation / miRNA-mediated post-transcriptional gene silencing / PeBoW complex / miRNA-mediated gene silencing by inhibition of translation / ribosomal protein import into nucleus / negative regulation of formation of translation preinitiation complex / blastocyst formation / protein localization to nucleolus / TORC2 complex binding / rRNA methylation / maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / GAIT complex / A band / regulation of glycolytic process / regulation of reactive oxygen species metabolic process / regulation of G1 to G0 transition / positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / regulation of translation involved in cellular response to UV / protein-DNA complex disassembly / : / preribosome, small subunit precursor / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / mitotic metaphase chromosome alignment / negative regulation of cell-cell adhesion / regulation of cyclin-dependent protein serine/threonine kinase activity / homeostatic process / G1 to G0 transition / maturation of 5.8S rRNA / negative regulation of DNA replication / rRNA metabolic process / macrophage chemotaxis / lung morphogenesis / Protein hydroxylation / ribosomal large subunit binding / negative regulation of ubiquitin protein ligase activity / Peptide chain elongation / rRNA transcription / Selenocysteine synthesis / positive regulation of signal transduction by p53 class mediator / Formation of a pool of free 40S subunits / nuclear-transcribed mRNA catabolic process / ubiquitin ligase inhibitor activity / Eukaryotic Translation Termination / preribosome, large subunit precursor / blastocyst development / Response of EIF2AK4 (GCN2) to amino acid deficiency / SRP-dependent cotranslational protein targeting to membrane / Viral mRNA Translation / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / GTP hydrolysis and joining of the 60S ribosomal subunit / L13a-mediated translational silencing of Ceruloplasmin expression / Major pathway of rRNA processing in the nucleolus and cytosol / cellular response to interleukin-4 / ribonucleoprotein complex binding / hematopoietic progenitor cell differentiation / cellular response to actinomycin D / negative regulation of ubiquitin-dependent protein catabolic process / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / ribosomal subunit export from nucleus / rough endoplasmic reticulum / MDM2/MDM4 family protein binding / cytosolic ribosome / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / : / maturation of LSU-rRNA / negative regulation of protein ubiquitination / translation initiation factor activity / nuclear periphery / Transferases; Transferring one-carbon groups; Methyltransferases / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / assembly of large subunit precursor of preribosome / negative regulation of cell migration / regulation of signal transduction by p53 class mediator / cytosolic ribosome assembly / condensed nuclear chromosome / ribosomal large subunit biogenesis / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / mRNA 3'-UTR binding / positive regulation of translation / response to insulin / molecular condensate scaffold activity / bone development / cellular response to gamma radiation / transcription coactivator binding / cellular response to type II interferon / mRNA 5'-UTR binding / Regulation of expression of SLITs and ROBOs / cytoplasmic ribonucleoprotein granule / osteoblast differentiation / rRNA processing

Domain/homology:

Ribosomal RNA-processing protein 15 / Protein Peter Pan-like / Rrp15p / Surfeit locus 6 / Ribosomal RNA-processing protein 14/surfeit locus protein 6, C-terminal domain / Surfeit locus protein 6 / MKI67 FHA domain-interacting nucleolar phosphoprotein, FHA Ki67 binding / FHA Ki67 binding domain of hNIFK / Ribosomal RNA methyltransferase, SPB1-like, C-terminal / Ribosomal RNA methyltransferase Spb1, domain of unknown function DUF3381 / AdoMet-dependent rRNA methyltransferase SPB1-like / Spb1 C-terminal domain / Ribosomal RNA methyltransferase Spb1, DUF3381 / Ribosomal biogenesis regulatory protein / Ribosome biogenesis regulatory protein (RRS1) / Eukaryotic rRNA processing / Eukaryotic rRNA processing protein EBP2 / : / Ribosomal RNA large subunit methyltransferase E / Ribosome biogenesis protein BRX1 / DDX18/Has1, DEAD-box helicase domain / Ribosome biogenesis protein Nop16 / Ribosome biogenesis protein Nop16 / Domain of unknown function DUF4217 / ATP-dependent rRNA helicase SPB4-like, C-terminal extension / DUF4217 / BOP1, N-terminal domain / WD repeat BOP1/Erb1 / BOP1NT (NUC169) domain / BOP1NT (NUC169) domain / Pescadillo / Pescadillo N-terminus / Ribosomal protein L24e / Ribosomal biogenesis NSA2 family / Ribosome assembly factor Mrt4 / : / BRCT domain / NOG, C-terminal / Nucleolar GTP-binding protein 1 / NOGCT (NUC087) domain / Nucleolar GTP-binding protein 1, Rossman-fold domain / NOG1, N-terminal helical domain / Nucleolar GTP-binding protein 1 (NOG1) / NOG1 N-terminal helical domain / Brix domain / Brix domain / Brix domain profile. / Brix / OBG-type guanine nucleotide-binding (G) domain / OBG-type guanine nucleotide-binding (G) domain profile. / Translation initiation factor IF6 / eIF-6 family / translation initiation factor 6 / DEAD-box subfamily ATP-dependent helicases signature. / ATP-dependent RNA helicase DEAD-box, conserved site / DEAD-box RNA helicase Q motif profile. / Ribosomal protein L6, N-terminal / Ribosomal protein L6, N-terminal domain / GTP binding domain / Ribosomal L15/L27a, N-terminal / Ribosomal protein L28e / 50S ribosomal protein L10, insertion domain superfamily / Ribosomal protein L23 / Ribosomal L28e/Mak16 / Ribosomal L28e protein family / 60S ribosomal protein L10P, insertion domain / Insertion domain in 60S ribosomal protein L10P / metallochaperone-like domain / breast cancer carboxy-terminal domain / TRASH domain / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / Ribosomal protein L13e / Ribosomal protein L13e / Ribosomal protein L1, 3-layer alpha/beta-sandwich / 60S ribosomal protein L18a/ L20, eukaryotes / Ribosomal protein L24e, conserved site / Ribosomal protein L24e signature. / : / Ribosomal protein L6e signature. / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal protein L23/L25, N-terminal / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / Ribosomal protein L23, N-terminal domain / Ribosomal protein 60S L18 and 50S L18e / Ribosomal protein L18/L18-A/B/e, conserved site / Ribosomal protein L18e signature. / Ribosomal protein L36e signature. / Ribosomal protein L35Ae, conserved site / Ribosomal protein L35Ae signature. / BRCT domain profile. / Ribosomal Protein L6, KOW domain / Ribosomal protein L7A/L8 / Ribosomal protein L1-like / Ribosomal protein L1/ribosomal biogenesis protein / Ribosomal protein L1p/L10e family / BRCT domain / Ribosomal RNA methyltransferase, FtsJ domain / FtsJ-like methyltransferase

Component:

Pescadillo homolog / Surfeit locus protein 6 / Ribosomal L1 domain-containing protein 1 / Ribosome biogenesis protein NSA2 homolog / Large ribosomal subunit protein eL33 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein eL13 / Large ribosomal subunit protein uL11 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein uL29 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein eL21 / Large ribosomal subunit protein eL28 / Large ribosomal subunit protein eL14 / Eukaryotic translation initiation factor 6 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein eL15 / Large ribosomal subunit protein eL37 / Large ribosomal subunit protein eL8 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein eL32 / Large ribosomal subunit protein eL20 / Large ribosomal subunit protein eL6 / Large ribosomal subunit protein eL18 / Ribosome biogenesis protein BOP1 / Ribosome biogenesis regulatory protein homolog / Ribosomal protein uL30-like / pre-rRNA 2'-O-ribose RNA methyltransferase FTSJ3 / Ribosome biogenesis protein BRX1 homolog / Probable rRNA-processing protein EBP2 / MKI67 FHA domain-interacting nucleolar phosphoprotein / GTP-binding protein 4 / Suppressor of SWI4 1 homolog / ATP-dependent RNA helicase DDX18 / Probable ribosome biogenesis protein RLP24 / mRNA turnover protein 4 homolog / RRP15-like protein / Nucleolar protein 16 / Large ribosomal subunit protein eL36

• Biological species: Homo sapiens (human) / human (human)
• Method: single particle reconstruction / cryo EM / Resolution: 2.88 Å
• Authors: Vanden Broeck A / Klinge S

Funding support

European Union, United States, 2 items

Organization	Grant number	Country
European Molecular Biology Organization (EMBO)	ALTF 711-2019	European Union
The G. Harold and Leila Y. Mathers Foundation	MF-2104-01554	United States

• Citation: Journal: Science / Year: 2023; Title: Principles of human pre-60 biogenesis.; Authors: Arnaud Vanden Broeck / Sebastian Klinge / ; Abstract: During the early stages of human large ribosomal subunit (60) biogenesis, an ensemble of assembly factors establishes and fine-tunes the essential RNA functional centers of pre-60 particles by an unknown mechanism. Here, we report a series of cryo-electron microscopy structures of human nucleolar and nuclear pre-60 assembly intermediates at resolutions of 2.5 to 3.2 angstroms. These structures show how protein interaction hubs tether assembly factor complexes to nucleolar particles and how guanosine triphosphatases and adenosine triphosphatase couple irreversible nucleotide hydrolysis steps to the installation of functional centers. Nuclear stages highlight how a conserved RNA-processing complex, the rixosome, couples large-scale RNA conformational changes with pre-ribosomal RNA processing by the RNA degradation machinery. Our ensemble of human pre-60 particles provides a rich foundation with which to elucidate the molecular principles of ribosome formation.

History

Deposition	Dec 21, 2022	-
Header (metadata) release	Jul 12, 2023	-
Map release	Jul 12, 2023	-
Update	Jul 19, 2023	-
Current status	Jul 19, 2023	Processing site: RCSB / Status: Released

Map

• File: Download / File: emd_29255.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 1.072 Å

Density

Contour Level	By AUTHOR: 0.75
Minimum - Maximum	-0.032373518 - 5.3902745
Average (Standard dev.)	0.04140359 (±0.15155604)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 480 480 480 Spacing 480 480 480
Cell	A=B=C: 514.56 Å α=β=γ: 90.0 °

Supplemental data

Mask #1

• File: emd_29255_msk_1.map

Half map: #2

• File: emd_29255_half_map_1.map

Half map: #1

• File: emd_29255_half_map_2.map

Sample components

Entire : Human nucleolar pre-60S ribosomal subunit (State B2)

• Entire: Name: Human nucleolar pre-60S ribosomal subunit (State B2)

Components

• Complex: Human nucleolar pre-60S ribosomal subunit (State B2)
  • Protein or peptide: 60S ribosomal protein L12
  • RNA: 5.8S rRNA
  • RNA: ITS2 rRNA
  • RNA: 28S rRNA
  • Protein or peptide: 60S ribosomal protein L13
  • Protein or peptide: 60S ribosomal protein L13a
  • Protein or peptide: 60S ribosomal protein L14
  • Protein or peptide: 60S ribosomal protein L15
  • Protein or peptide: 60S ribosomal protein L17
  • Protein or peptide: 60S ribosomal protein L18
  • Protein or peptide: 60S ribosomal protein L18a
  • Protein or peptide: 60S ribosomal protein L21
  • Protein or peptide: 60S ribosomal protein L23
  • Protein or peptide: 60S ribosomal protein L23a
  • Protein or peptide: 60S ribosomal protein L26
  • Protein or peptide: 60S ribosomal protein L27a
  • Protein or peptide: 60S ribosomal protein L28
  • Protein or peptide: 60S ribosomal protein L3
  • Protein or peptide: 60S ribosomal protein L32
  • Protein or peptide: 60S ribosomal protein L35
  • Protein or peptide: 60S ribosomal protein L35a
  • Protein or peptide: 60S ribosomal protein L36
  • Protein or peptide: 60S ribosomal protein L37
  • Protein or peptide: Surfeit locus protein 6
  • Protein or peptide: Ribosome biogenesis protein NSA2 homolog
  • Protein or peptide: RRP15-like protein
  • Protein or peptide: Suppressor of SWI4 1 homolog
  • Protein or peptide: 60S ribosomal protein L4
  • Protein or peptide: 60S ribosomal protein L6
  • Protein or peptide: 60S ribosomal protein L7
  • Protein or peptide: 60S ribosomal protein L7a
  • Protein or peptide: 60S ribosomal protein L9
  • Protein or peptide: MKI67 FHA domain-interacting nucleolar phosphoprotein
  • Protein or peptide: 60S ribosomal protein L7-like 1
  • Protein or peptide: pre-rRNA 2'-O-ribose RNA methyltransferase FTSJ3
  • Protein or peptide: Eukaryotic translation initiation factor 6
  • Protein or peptide: Ribosomal L1 domain-containing protein 1
  • Protein or peptide: Pescadillo homolog
  • Protein or peptide: Probable rRNA-processing protein EBP2
  • Protein or peptide: Ribosome biogenesis protein BRX1 homolog
  • Protein or peptide: mRNA turnover protein 4 homolog
  • Protein or peptide: GTP-binding protein 4
  • Protein or peptide: Ribosome biogenesis protein BOP1
  • Protein or peptide: Ribosome biogenesis regulatory protein homolog
  • Protein or peptide: Probable ribosome biogenesis protein RLP24
  • Protein or peptide: ATP-dependent RNA helicase DDX18
  • Protein or peptide: Nucleolar protein 16
• Ligand: MAGNESIUM ION
• Ligand: ZINC ION
• Ligand: GUANOSINE-5'-DIPHOSPHATE
• Ligand: POTASSIUM ION

Supramolecule #1: Human nucleolar pre-60S ribosomal subunit (State B2)

• Supramolecule: Name: Human nucleolar pre-60S ribosomal subunit (State B2) / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#47
• Source (natural): Organism: Homo sapiens (human) / Cell: HEK293F

Macromolecule #1: 60S ribosomal protein L12

• Macromolecule: Name: 60S ribosomal protein L12 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 17.847619 KDa

Sequence

String:

MPPKFDPNEI KVVYLRCTGG EVGATSALAP KIGPLGLSPK KVGDDIAKAT GDWKGLRITV KLTIQNRQAQ IEVVPSASAL IIKALKEPP RDRKKQKNIK HSGNITFDEI VNIARQMRHR SLARELSGTI KEILGTAQSV GCNVDGRHPH DIIDDINSGA V ECPAS

UniProtKB: Large ribosomal subunit protein uL11

Macromolecule #5: 60S ribosomal protein L13

• Macromolecule: Name: 60S ribosomal protein L13 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 24.321682 KDa

Sequence

String:

MAPSRNGMVL KPHFHKDWQR RVATWFNQPA RKIRRRKARQ AKARRIAPRP ASGPIRPIVR CPTVRYHTKV RAGRGFSLEE LRVAGIHKK VARTIGISVD PRRRNKSTES LQANVQRLKE YRSKLILFPR KPSAPKKGDS SAEELKLATQ LTGPVMPVRN V YKKEKARV ITEEEKNFKA FASLRMARAN ARLFGIRAKR AKEAAEQDVE KKK

UniProtKB: Large ribosomal subunit protein eL13

Macromolecule #6: 60S ribosomal protein L13a

• Macromolecule: Name: 60S ribosomal protein L13a / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 23.633412 KDa

Sequence

String:

MAEVQVLVLD GRGHLLGRLA AIVAKQVLLG RKVVVVRCEG INISGNFYRN KLKYLAFLRK RMNTNPSRGP YHFRAPSRIF WRTVRGMLP HKTKRGQAAL DRLKVFDGIP PPYDKKKRMV VPAALKVVRL KPTRKFAYLG RLAHEVGWKY QAVTATLEEK R KEKAKIHY RKKKQLMRLR KQAEKNVEKK IDKYTEVLKT HGLLV

UniProtKB: Large ribosomal subunit protein uL13

Macromolecule #7: 60S ribosomal protein L14

• Macromolecule: Name: 60S ribosomal protein L14 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 23.485016 KDa

Sequence

String:

MVFRRFVEVG RVAYVSFGPH AGKLVAIVDV IDQNRALVDG PCTQVRRQAM PFKCMQLTDF ILKFPHSAHQ KYVRQAWQKA DINTKWAAT RWAKKIEARE RKAKMTDFDR FKVMKAKKMR NRIIKNEVKK LQKAALLKAS PKKAPGTKGT AAAAAAAAAA K VPAKKITA ASKKAPAQKV PAQKATGQKA APAPKAQKGQ KAPAQKAPAP KASGKKA

UniProtKB: Large ribosomal subunit protein eL14

Macromolecule #8: 60S ribosomal protein L15

• Macromolecule: Name: 60S ribosomal protein L15 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 24.207285 KDa

Sequence

String:

MGAYKYIQEL WRKKQSDVMR FLLRVRCWQY RQLSALHRAP RPTRPDKARR LGYKAKQGYV IYRIRVRRGG RKRPVPKGAT YGKPVHHGV NQLKFARSLQ SVAEERAGRH CGALRVLNSY WVGEDSTYKF FEVILIDPFH KAIRRNPDTQ WITKPVHKHR E MRGLTSAG RKSRGLGKGH KFHHTIGGSR RAAWRRRNTL QLHRYR

UniProtKB: Large ribosomal subunit protein eL15

Macromolecule #9: 60S ribosomal protein L17

• Macromolecule: Name: 60S ribosomal protein L17 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 21.44317 KDa

Sequence

String:

MVRYSLDPEN PTKSCKSRGS NLRVHFKNTR ETAQAIKGMH IRKATKYLKD VTLQKQCVPF RRYNGGVGRC AQAKQWGWTQ GRWPKKSAE FLLHMLKNAE SNAELKGLDV DSLVIEHIQV NKAPKMRRRT YRAHGRINPY MSSPCHIEMI LTEKEQIVPK P EEEVAQKK KISQKKLKKQ KLMARE

UniProtKB: Large ribosomal subunit protein uL22

Macromolecule #10: 60S ribosomal protein L18

• Macromolecule: Name: 60S ribosomal protein L18 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 21.687676 KDa

Sequence

String:

MGVDIRHNKD RKVRRKEPKS QDIYLRLLVK LYRFLARRTN STFNQVVLKR LFMSRTNRPP LSLSRMIRKM KLPGRENKTA VVVGTITDD VRVQEVPKLK VCALRVTSRA RSRILRAGGK ILTFDQLALD SPKGCGTVLL SGPRKGREVY RHFGKAPGTP H SHTKPYVR SKGRKFERAR GRRASRGYKN

UniProtKB: Large ribosomal subunit protein eL18

Macromolecule #11: 60S ribosomal protein L18a

• Macromolecule: Name: 60S ribosomal protein L18a / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 20.808514 KDa

Sequence

String:

MKASGTLREY KVVGRCLPTP KCHTPPLYRM RIFAPNHVVA KSRFWYFVSQ LKKMKKSSGE IVYCGQVFEK SPLRVKNFGI WLRYDSRSG THNMYREYRD LTTAGAVTQC YRDMGARHRA RAHSIQIMKV EEIAASKCRR PAVKQFHDSK IKFPLPHRVL R RQHKPRFT TKRPNTFF

UniProtKB: Large ribosomal subunit protein eL20

Macromolecule #12: 60S ribosomal protein L21

• Macromolecule: Name: 60S ribosomal protein L21 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 18.609988 KDa

Sequence

String:

MTNTKGKRRG TRYMFSRPFR KHGVVPLATY MRIYKKGDIV DIKGMGTVQK GMPHKCYHGK TGRVYNVTQH AVGIVVNKQV KGKILAKRI NVRIEHIKHS KSRDSFLKRV KENDQKKKEA KEKGTWVQLK RQPAPPREAH FVRTNGKEPE LLEPIPYEFM A

UniProtKB: Large ribosomal subunit protein eL21

Macromolecule #13: 60S ribosomal protein L23

• Macromolecule: Name: 60S ribosomal protein L23 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 14.892505 KDa

Sequence

String:

MSKRGRGGSS GAKFRISLGL PVGAVINCAD NTGAKNLYII SVKGIKGRLN RLPAAGVGDM VMATVKKGKP ELRKKVHPAV VIRQRKSYR RKDGVFLYFE DNAGVIVNNK GEMKGSAITG PVAKECADLW PRIASNAGSI A

UniProtKB: Large ribosomal subunit protein uL14

Macromolecule #14: 60S ribosomal protein L23a

• Macromolecule: Name: 60S ribosomal protein L23a / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 17.740193 KDa

Sequence

String:

MAPKAKKEAP APPKAEAKAK ALKAKKAVLK GVHSHKKKKI RTSPTFRRPK TLRLRRQPKY PRKSAPRRNK LDHYAIIKFP LTTESAMKK IEDNNTLVFI VDVKANKHQI KQAVKKLYDI DVAKVNTLIR PDGEKKAYVR LAPDYDALDV ANKIGII

UniProtKB: Large ribosomal subunit protein uL23

Macromolecule #15: 60S ribosomal protein L26

• Macromolecule: Name: 60S ribosomal protein L26 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 17.303363 KDa

Sequence

String:

MKFNPFVTSD RSKNRKRHFN APSHIRRKIM SSPLSKELRQ KYNVRSMPIR KDDEVQVVRG HYKGQQIGKV VQVYRKKYVI YIERVQREK ANGTTVHVGI HPSKVVITRL KLDKDRKKIL ERKAKSRQVG KEKGKYKEET IEKMQE

UniProtKB: Large ribosomal subunit protein uL24

Macromolecule #16: 60S ribosomal protein L27a

• Macromolecule: Name: 60S ribosomal protein L27a / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 16.604535 KDa

Sequence

String:

MPSRLRKTRK LRGHVSHGHG RIGKHRKHPG GRGNAGGLHH HRINFDKYHP GYFGKVGMKH YHLKRNQSFC PTVNLDKLWT LVSEQTRVN AAKNKTGAAP IIDVVRSGYY KVLGKGKLPK QPVIVKAKFF SRRAEEKIKS VGGACVLVA

UniProtKB: Large ribosomal subunit protein uL15

Macromolecule #17: 60S ribosomal protein L28

• Macromolecule: Name: 60S ribosomal protein L28 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 15.784622 KDa

Sequence

String:

MSAHLQWMVV RNCSSFLIKR NKQTYSTEPN NLKARNSFRY NGLIHRKTVG VEPAADGKGV VVVIKRRSGQ RKPATSYVRT TINKNARAT LSSIRHMIRK NKYRPDLRMA AIRRASAILR SQKPVMVKRK RTRPTKSS

UniProtKB: Large ribosomal subunit protein eL28

Macromolecule #18: 60S ribosomal protein L3

• Macromolecule: Name: 60S ribosomal protein L3 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 46.224133 KDa

Sequence

String:

MSHRKFSAPR HGSLGFLPRK RSSRHRGKVK SFPKDDPSKP VHLTAFLGYK AGMTHIVREV DRPGSKVNKK EVVEAVTIVE TPPMVVVGI VGYVETPRGL RTFKTVFAEH ISDECKRRFY KNWHKSKKKA FTKYCKKWQD EDGKKQLEKD FSSMKKYCQV I RVIAHTQM RLLPLRQKKA HLMEIQVNGG TVAEKLDWAR ERLEQQVPVN QVFGQDEMID VIGVTKGKGY KGVTSRWHTK KL PRKT(HIC)RG LRKVACIGAW HPARVAFSVA RAGQKGYHHR TEINKKIYKI GQGYLIKDGK LIKNNASTDY DLSDKSINP LGGFVHYGEV TNDFVMLKGC VVGTKKRVLT LRKSLLVQTK RRALEKIDLK FIDTTSKFGH GRFQTMEEKK AFMGPLKKDR IAKEEGA

UniProtKB: Large ribosomal subunit protein uL3

Macromolecule #19: 60S ribosomal protein L32

• Macromolecule: Name: 60S ribosomal protein L32 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 15.898932 KDa

Sequence

String:

MAALRPLVKP KIVKKRTKKF IRHQSDRYVK IKRNWRKPRG IDNRVRRRFK GQILMPNIGY GSNKKTKHML PSGFRKFLVH NVKELEVLL MCNKSYCAEI AHNVSSKNRK AIVERAAQLA IRVTNPNARL RSEENE

UniProtKB: Large ribosomal subunit protein eL32

Macromolecule #20: 60S ribosomal protein L35

• Macromolecule: Name: 60S ribosomal protein L35 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 14.593624 KDa

Sequence

String:

MAKIKARDLR GKKKEELLKQ LDDLKVELSQ LRVAKVTGGA ASKLSKIRVV RKSIARVLTV INQTQKENLR KFYKGKKYKP LDLRPKKTR AMRRRLNKHE ENLKTKKQQR KERLYPLRKY AVKA

UniProtKB: Large ribosomal subunit protein uL29

Macromolecule #21: 60S ribosomal protein L35a

• Macromolecule: Name: 60S ribosomal protein L35a / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 12.564743 KDa

Sequence

String:

MSGRLWSKAI FAGYKRGLRN QREHTALLKI EGVYARDETE FYLGKRCAYV YKAKNNTVTP GGKPNKTRVI WGKVTRAHGN SGMVRAKFR SNLPAKAIGH RIRVMLYPSR I

UniProtKB: Large ribosomal subunit protein eL33

Macromolecule #22: 60S ribosomal protein L36

• Macromolecule: Name: 60S ribosomal protein L36 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 12.290859 KDa

Sequence

String:

MALRYPMAVG LNKGHKVTKN VSKPRHSRRR GRLTKHTKFV RDMIREVCGF APYERRAMEL LKVSKDKRAL KFIKKRVGTH IRAKRKREE LSNVLAAMRK AAAKKD

UniProtKB: Large ribosomal subunit protein eL36

Macromolecule #23: 60S ribosomal protein L37

• Macromolecule: Name: 60S ribosomal protein L37 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 11.111032 KDa

Sequence

String:

MTKGTSSFGK RRNKTHTLCR RCGSKAYHLQ KSTCGKCGYP AKRKRKYNWS AKAKRRNTTG TGRMRHLKIV YRRFRHGFRE GTTPKPKRA AVAASSSS

UniProtKB: Large ribosomal subunit protein eL37

Macromolecule #24: Surfeit locus protein 6

• Macromolecule: Name: Surfeit locus protein 6 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 41.553758 KDa

Sequence

String:

MASLLAKDAY LQSLAKKICS HSAPEQQART RAGKTQGSET AGPPKKKRKK TQKKFRKREE KAAEHKAKSL GEKSPAASGA RRPEAAKEE AAWASSSAGN PADGLATEPE SVFALDVLRQ RLHEKIQEAR GQGSAKELSP AALEKRRRRK QERDRKKRKR K ELRAKEKA RKAEEATEAQ EVVEATPEGA CTEPREPPGL IFNKVEVSED EPASKAQRRK EKRQRVKGNL TPLTGRNYRQ LL ERLQARQ SRLDELRGQD EGKAQELEAK MKWTNLLYKA EGVKIRDDER LLQEALKRKE KRRAQRQRRW EKRTAGVVEK MQQ RQDRRR QNLRRKKAAR AERRLLRARK KGRILPQDLE RAGLV

UniProtKB: Surfeit locus protein 6

Macromolecule #25: Ribosome biogenesis protein NSA2 homolog

• Macromolecule: Name: Ribosome biogenesis protein NSA2 homolog / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 30.136703 KDa

Sequence

String:

MPQNEYIELH RKRYGYRLDY HEKKRKKESR EAHERSKKAK KMIGLKAKLY HKQRHAEKIQ MKKTIKMHEK RNTKQKNDEK TPQGAVPAY LLDREGQSRA KVLSNMIKQK RKEKAGKWEV PLPKVRAQGE TEVLKVIRTG KRKKKAWKRM VTKVCFVGDG F TRKPPKYE RFIRPMGLRF KKAHVTHPEL KATFCLPILG VKKNPSSPLY TTLGVITKGT VIEVNVSELG LVTQGGKVIW GK YAQVTNN PENDGCINAV LLV

UniProtKB: Ribosome biogenesis protein NSA2 homolog

Macromolecule #26: RRP15-like protein

• Macromolecule: Name: RRP15-like protein / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 31.539318 KDa

Sequence

String:

MAAAAPDSRV SEEENLKKTP KKKMKMVTGA VASVLEDEAT DTSDSEGSCG SEKDHFYSDD DAIEADSEGD AEPCDKENEN DGESSVGTN MGWADAMAKV LNKKTPESKP TILVKNKKLE KEKEKLKQER LEKIKQRDKR LEWEMMCRVK PDVVQDKETE R NLQRIATR GVVQLFNAVQ KHQKNVDEKV KEAGSSMRKR AKLISTVSKK DFISVLRGMD GSTNETASSR KKPKAKQTEV KS EEGPGWT ILRDDFMMGA SMKDWDKESD GPDDSRPESA SDSDT

UniProtKB: RRP15-like protein

Macromolecule #27: Suppressor of SWI4 1 homolog

• Macromolecule: Name: Suppressor of SWI4 1 homolog / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 53.292102 KDa

Sequence

String:

MGQSGRSRHQ KRARAQAQLR NLEAYAANPH SFVFTRGCTG RNIRQLSLDV RRVMEPLTAS RLQVRKKNSL KDCVAVAGPL GVTHFLILS KTETNVYFKL MRLPGGPTLT FQVKKYSLVR DVVSSLRRHR MHEQQFAHPP LLVLNSFGPH GMHVKLMATM F QNLFPSIN VHKVNLNTIK RCLLIDYNPD SQELDFRHYS IKVVPVGASR GMKKLLQEKF PNMSRLQDIS ELLATGAGLS ES EAEPDGD HNITELPQAV AGRGNMRAQQ SAVRLTEIGP RMTLQLIKVQ EGVGEGKVMF HSFVSKTEEE LQAILEAKEK KLR LKAQRQ AQQAQNVQRK QEQREAHRKK SLEGMKKARV GGSDEEASGI PSRTASLELG EDDDEQEDDD IEYFCQAVGE APSE DLFPE AKQKRLAKSP GRKRKRWEMD RGRGRLCDQK FPKTKDKSQG AQARRGPRGA SRDGGRGRGR GRPGKRVA

UniProtKB: Suppressor of SWI4 1 homolog

Macromolecule #28: 60S ribosomal protein L4

• Macromolecule: Name: 60S ribosomal protein L4 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 47.804621 KDa

Sequence

String:

MACARPLISV YSEKGESSGK NVTLPAVFKA PIRPDIVNFV HTNLRKNNRQ PYAVSELAGH QTSAESWGTG RAVARIPRVR GGGTHRSGQ GAFGNMCRGG RMFAPTKTWR RWHRRVNTTQ KRYAICSALA ASALPALVMS KGHRIEEVPE LPLVVEDKVE G YKKTKEAV LLLKKLKAWN DIKKVYASQR MRAGKGKMRN RRRIQRRGPC IIYNEDNGII KAFRNIPGIT LLNVSKLNIL KL APGGHVG RFCIWTESAF RKLDELYGTW RKAASLKSNY NLPMHKMINT DLSRILKSPE IQRALRAPRK KIHRRVLKKN PLK NLRIML KLNPYAKTMR RNTILRQARN HKLRVDKAAA AAAALQAKSD EKAAVAGKKP VVGKKGKKAA VGVKKQKKPL VGKK AAATK KPAPEKKPAE KKPTTEEKKP AA

UniProtKB: Large ribosomal subunit protein uL4

Macromolecule #29: 60S ribosomal protein L6

• Macromolecule: Name: 60S ribosomal protein L6 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 32.810176 KDa

Sequence

String:

MAGEKVEKPD TKEKKPEAKK VDAGGKVKKG NLKAKKPKKG KPHCSRNPVL VRGIGRYSRS AMYSRKAMYK RKYSAAKSKV EKKKKEKVL ATVTKPVGGD KNGGTRVVKL RKMPRYYPTE DVPRKLLSHG KKPFSQHVRK LRASITPGTI LIILTGRHRG K RVVFLKQL ASGLLLVTGP LVLNRVPLRR THQKFVIATS TKIDISNVKI PKHLTDAYFK KKKLRKPRHQ EGEIFDTEKE KY EITEQRK IDQKAVDSQI LPKIKAIPQL QGYLRSVFAL TNGIYPHKLV F

UniProtKB: Large ribosomal subunit protein eL6

Macromolecule #30: 60S ribosomal protein L7

• Macromolecule: Name: 60S ribosomal protein L7 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 29.290973 KDa

Sequence

String:

MEGVEEKKKE VPAVPETLKK KRRNFAELKI KRLRKKFAQK MLRKARRKLI YEKAKHYHKE YRQMYRTEIR MARMARKAGN FYVPAEPKL AFVIRIRGIN GVSPKVRKVL QLLRLRQIFN GTFVKLNKAS INMLRIVEPY IAWGYPNLKS VNELIYKRGY G KINKKRIA LTDNALIARS LGKYGIICME DLIHEIYTVG KRFKEANNFL WPFKLSSPRG GMKKKTTHFV EGGDAGNRED QI NRLIRRM N

UniProtKB: Large ribosomal subunit protein uL30

Macromolecule #31: 60S ribosomal protein L7a

• Macromolecule: Name: 60S ribosomal protein L7a / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 30.061785 KDa

Sequence

String:

MPKGKKAKGK KVAPAPAVVK KQEAKKVVNP LFEKRPKNFG IGQDIQPKRD LTRFVKWPRY IRLQRQRAIL YKRLKVPPAI NQFTQALDR QTATQLLKLA HKYRPETKQE KKQRLLARAE KKAAGKGDVP TKRPPVLRAG VNTVTTLVEN KKAQLVVIAH D VDPIELVV FLPALCRKMG VPYCIIKGKA RLGRLVHRKT CTTVAFTQVN SEDKGALAKL VEAIRTNYND RYDEIRRHWG GN VLGPKSV ARIAKLEKAK AKELATKLG

UniProtKB: Large ribosomal subunit protein eL8

Macromolecule #32: 60S ribosomal protein L9

• Macromolecule: Name: 60S ribosomal protein L9 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 21.899471 KDa

Sequence

String:

MKTILSNQTV DIPENVDITL KGRTVIVKGP RGTLRRDFNH INVELSLLGK KKKRLRVDKW WGNRKELATV RTICSHVQNM IKGVTLGFR YKMRSVYAHF PINVVIQENG SLVEIRNFLG EKYIRRVRMR PGVACSVSQA QKDELILEGN DIELVSNSAA L IQQATTVK NKDIRKFLDG IYVSEKGTVQ QADE

UniProtKB: Large ribosomal subunit protein uL6

Macromolecule #33: MKI67 FHA domain-interacting nucleolar phosphoprotein

• Macromolecule: Name: MKI67 FHA domain-interacting nucleolar phosphoprotein / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 34.285309 KDa

Sequence

String:

MATFSGPAGP ILSLNPQEDV EFQKEVAQVR KRITQRKKQE QLTPGVVYVR HLPNLLDETQ IFSYFSQFGT VTRFRLSRSK RTGNSKGYA FVEFESEDVA KIVAETMNNY LFGERLLECH FMPPEKVHKE LFKDWNIPFK QPSYPSVKRY NRNRTLTQKL R MEERFKKK ERLLRKKLAK KGIDYDFPSL ILQKTESISK TNRQTSTKGQ VLRKKKKKVS GTLDTPEKTV DSQGPTPVCT PT FLERRKS QVAELNDDDK DDEIVFKQPI SCVKEEIQET QTPTHSRKKR RRSSNQ

UniProtKB: MKI67 FHA domain-interacting nucleolar phosphoprotein

Macromolecule #34: 60S ribosomal protein L7-like 1

• Macromolecule: Name: 60S ribosomal protein L7-like 1 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 29.730354 KDa

Sequence

String:

MISSCTTRKM AEQEQRKIPL VPENLLKKRK AYQALKATQA KQALLAKKEQ KKGKGLRFKR LESFLHDSWR QKRDKVRLRR LEVKPHALE LPDKHSLAFV VRIERIDGVS LLVQRTIARL RLKKIFSGVF VKVTPQNLKM LRIVEPYVTW GFPNLKSVRE L ILKRGQAK VKNKTIPLTD NTVIEEHLGK FGVICLEDLI HEIAFPGKHF QEISWFLCPF HLSVARHATK NRVGFLKEMG TP GYRGERI NQLIRQLN

UniProtKB: Ribosomal protein uL30-like

Macromolecule #35: pre-rRNA 2'-O-ribose RNA methyltransferase FTSJ3

• Macromolecule: Name: pre-rRNA 2'-O-ribose RNA methyltransferase FTSJ3 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO; EC number: Transferases; Transferring one-carbon groups; Methyltransferases
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 96.726875 KDa

Sequence

String:

MGKKGKVGKS RRDKFYHLAK ETGYRSRSAF KLIQLNRRFQ FLQKARALLD LCAAPGGWLQ VAAKFMPVSS LIVGVDLVPI KPLPNVVTL QQDITTERCR QALRKELKTW KVDVVLNDGA PNVGASWVHD AYSQAHLTLM ALRLACDFLA RGGSFITKVF R SRDYQPLL WIFQQLFRRV QATKPQASRH ESAEIFVVCQ GFLAPDKVDS KFFDPKFAFK EVEVQAKTVT ELVTKKKPKA EG YAEGDLT LYHRTSVTDF LRAANPVDFL SKASEIMVDD EELAQHPATT EDIRVCCQDI RVLGRKELRS LLNWRTKLRR YVA KKLKEQ AKALDISLSS GEEDEGDEED STAGTTKQPS KEEEEEEEEE QLNQTLAEMK AQEVAELKRK KKKLLREQRK QRER VELKM DLPGVSIADE GETGMFSLST IRGHQLLEEV TQGDMSAADT FLSDLPRDDI YVSDVEDDGD DTSLDSDLDP EELAG VRGH QGLRDQKRMR LTEVQDDKEE EEEENPLLVP LEEKAVLQEE QANLWFSKGS FAGIEDDADE ALEISQAQLL FENRRK GRQ QQQKQQLPQT PPSCLKTEIM SPLYQDEAPK GTEASSGTEA ATGLEGEEKD GISDSDSSTS SEEEESWEPL RGKKRSR GP KSDDDGFEIV PIEDPAKHRI LDPEGLALGA VIASSKKAKR DLIDNSFNRY TFNEDEGELP EWFVQEEKQH RIRQLPVG K KEVEHYRKRW REINARPIKK VAEAKARKKR RMLKRLEQTR KKAEAVVNTV DISEREKVAQ LRSLYKKAGL GKEKRHVTY VVAKKGVGRK VRRPAGVRGH FKVVDSRMKK DQRAQQRKEQ KKKHKRK

UniProtKB: pre-rRNA 2'-O-ribose RNA methyltransferase FTSJ3

Macromolecule #36: Eukaryotic translation initiation factor 6

• Macromolecule: Name: Eukaryotic translation initiation factor 6 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 26.62001 KDa

Sequence

String:

MAVRASFENN CEIGCFAKLT NTYCLVAIGG SENFYSVFEG ELSDTIPVVH ASIAGCRIIG RMCVGNRHGL LVPNNTTDQE LQHIRNSLP DTVQIRRVEE RLSALGNVTT CNDYVALVHP DLDRETEEIL ADVLKVEVFR QTVADQVLVG SYCVFSNQGG L VHPKTSIE DQDELSSLLQ VPLVAGTVNR GSEVIAAGMV VNDWCAFCGL DTTSTELSVV ESVFKLNEAQ PSTIATSMRD SL IDSLT

UniProtKB: Eukaryotic translation initiation factor 6

Macromolecule #37: Ribosomal L1 domain-containing protein 1

• Macromolecule: Name: Ribosomal L1 domain-containing protein 1 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 55.08975 KDa

Sequence

String:

MEDSASASLS SAAATGTSTS TPAAPTARKQ LDKEQVRKAV DALLTHCKSR KNNYGLLLNE NESLFLMVVL WKIPSKELRV RLTLPHSIR SDSEDICLFT KDEPNSTPEK TEQFYRKLLN KHGIKTVSQI ISLQTLKKEY KSYEAKLRLL SSFDFFLTDA R IRRLLPSL IGRHFYQRKK VPVSVNLLSK NLSREINDCI GGTVLNISKS GSCSAIRIGH VGMQIEHIIE NIVAVTKGLS EK LPEKWES VKLLFVKTEK SAALPIFSSF VSNWDEATKR SLLNKKKKEA RRKRRERNFE KQKERKKKRQ QARKTASVLS KDD VAPESG DTTVKKPESK KEQTPEHGKK KRGRGKAQVK ATNESEDEIP QLVPIGKKTP ANEKVEIQKH ATGKKSPAKS PNPS TPRGK KRKALPASET PKAAESETPG KSPEKKPKIK EEAVKEKSPS LGKKDARQTP KKPEAKFFTT PSKSVRKASH TPKKW PKKP KVPQST

UniProtKB: Ribosomal L1 domain-containing protein 1

Macromolecule #38: Pescadillo homolog

• Macromolecule: Name: Pescadillo homolog / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 68.114727 KDa

Sequence

String:

MGGLEKKKYE RGSATNYITR NKARKKLQLS LADFRRLCIL KGIYPHEPKH KKKVNKGSTA ARTFYLIKDI RFLLHEPIVN KFREYKVFV RKLRKAYGKS EWNTVERLKD NKPNYKLDHI IKERYPTFID ALRDLDDALS MCFLFSTFPR TGKCHVQTIQ L CRRLTVEF MHYIIAARAL RKVFLSIKGI YYQAEVLGQP IVWITPYAFS HDHPTDVDYR VMATFTEFYT TLLGFVNFRL YQ LLNLHYP PKLEGQAQAE AKAGEGTYAL DSESCMEKLA ALSASLARVV VPATEEEAEV DEFPTDGEMS AQEEDRRKEL EAQ EKHKKL FEGLKFFLNR EVPREALAFI IRSFGGEVSW DKSLCIGATY DVTDSRITHQ IVDRPGQQTS VIGRCYVQPQ WVFD SVNAR LLLPVAEYFS GVQLPPHLSP FVTEKEGDYV PPEKLKLLAL QRGEDPGNLN ESEEEEEEDD NNEGDGDEEG ENEEE EEDA EAGSEKEEEA RLAALEEQRM EGKKPRVMAG TLKLEDKQRL AQEEESEAKR LAIMMMKKRE KYLYQKIMFG KRRKIR EAN KLAEKRKAHD EAVRSEKKAK KARPE

UniProtKB: Pescadillo homolog

Macromolecule #39: Probable rRNA-processing protein EBP2

• Macromolecule: Name: Probable rRNA-processing protein EBP2 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 34.925145 KDa

Sequence

String:

MDTPPLSDSE SESDESLVTD RELQDAFSRG LLKPGLNVVL EGPKKAVNDV NGLKQCLAEF KRDLEWVERL DVTLGPVPEI GGSEAPAPQ NKDQKAVDPE DDFQREMSFY RQAQAAVLAV LPRLHQLKVP TKRPTDYFAE MAKSDLQMQK IRQKLQTKQA A MERSEKAK QLRALRKYGK KVQTEVLQKR QQEKAHMMNA IKKYQKGFSD KLDFLEGDQK PLAQRKKAGA KGQQMRKGPS AK RRYKNQK FGFGGKKKGS KWNTRESYDD VSSFRAKTAH GRGLKRPGKK GSNKRPGKRT REKMKNRTH

UniProtKB: Probable rRNA-processing protein EBP2

Macromolecule #40: Ribosome biogenesis protein BRX1 homolog

• Macromolecule: Name: Ribosome biogenesis protein BRX1 homolog / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 41.483516 KDa

Sequence

String:

MAATKRKRRG GFAVQAKKPK RNEIDAEPPA KRHATAEEVE EEERDRIPGP VCKGKWKNKE RILIFSSRGI NFRTRHLMQD LRMLMPHSK ADTKMDRKDK LFVINEVCEM KNCNKCIYFE AKKKQDLYMW LSNSPHGPSA KFLVQNIHTL AELKMTGNCL K GSRPLLSF DPAFDELPHY ALLKELLIQI FSTPRYHPKS QPFVDHVFTF TILDNRIWFR NFQIIEEDAA LVEIGPRFVL NL IKIFQGS FGGPTLYENP HYQSPNMHRR VIRSITAAKY REKQQVKDVQ KLRKKEPKTL LPHDPTADVF VTPAEEKPIE IQW VKPEPK VDLKARKKRI YKRQRKMKQR MDSGKTK

UniProtKB: Ribosome biogenesis protein BRX1 homolog

Macromolecule #41: mRNA turnover protein 4 homolog

• Macromolecule: Name: mRNA turnover protein 4 homolog / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 27.602535 KDa

Sequence

String:

MPKSKRDKKV SLTKTAKKGL ELKQNLIEEL RKCVDTYKYL FIFSVANMRN SKLKDIRNAW KHSRMFFGKN KVMMVALGRS PSDEYKDNL HQVSKRLRGE VGLLFTNRTK EEVNEWFTKY TEMDYARAGN KAAFTVSLDP GPLEQFPHSM EPQLRQLGLP T ALKRGVVT LLSDYEVCKE GDVLTPEQAR VLKLFGYEMA EFKVTIKYMW DSQSGRFQQM GDDLPESASE STEESDSEDD D

UniProtKB: mRNA turnover protein 4 homolog

Macromolecule #42: GTP-binding protein 4

• Macromolecule: Name: GTP-binding protein 4 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 74.10782 KDa

Sequence

String:

MAHYNFKKIT VVPSAKDFID LTLSKTQRKT PTVIHKHYQI HRIRHFYMRK VKFTQQNYHD RLSQILTDFP KLDDIHPFYA DLMNILYDK DHYKLALGQI NIAKNLVDNV AKDYVRLMKY GDSLYRCKQL KRAALGRMCT VIKRQKQSLE YLEQVRQHLS R LPTIDPNT RTLLLCGYPN VGKSSFINKV TRADVDVQPY AFTTKSLFVG HMDYKYLRWQ VVDTPGILDH PLEDRNTIEM QA ITALAHL RAAVLYVMDL SEQCGHGLRE QLELFQNIRP LFINKPLIVV ANKCDVKRIA ELSEDDQKIF TDLQSEGFPV IET STLTEE GVIKVKTEAC DRLLAHRVET KMKGNKVNEV LNRLHLAIPT RRDDKERPPF IPEGVVARRK RMETEESRKK RERD LELEM GDDYILDLQK YWDLMNLSEK HDKIPEIWEG HNIADYIDPA IMKKLEELEK EEELRTAAGE YDSVSESEDE EMLEI RQLA KQIREKKKLK ILESKEKNTQ GPRMPRTAKK VQRTVLEKEM RSLGVDMDDK DDAHYAVQAR RSRSITRKRK REDSAP PSS VARSGSCSRT PRDVSGLRDV KMVKKAKTMM KNAQKKMNRL GKKGEADRHV FDMKPKHLLS GKRKAGKKDR R

UniProtKB: GTP-binding protein 4

Macromolecule #43: Ribosome biogenesis protein BOP1

• Macromolecule: Name: Ribosome biogenesis protein BOP1 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 83.901469 KDa

Sequence

String:

MAGSRGAGRT AAPSVRPEKR RSEPELEPEP EPEPPLLCTS PLSHSTGSDS GVSDSEESVF SGLEDSGSDS SEDDDEGDEE GEDGALDDE GHSGIKKTTE EQVQASTPCP RTEMASARIG DEYAED(SEP)(SEP)DE EDIRNTVGNV PLEWYDDFPH VGY DLDGRR IYKPLRTRDE LDQFLDKMDD PDYWRTVQDP MTGRDLRLTD EQVALVRRLQ SGQFGDVGFN PYEPAVDFFS GDVM IHPVT NRPADKRSFI PSLVEKEKVS RMVHAIKMGW IQPRRPRDPT PSFYDLWAQE DPNAVLGRHK MHVPAPKLAL PGHAE SYNP PPEYLLSEEE RLAWEQQEPG ERKLSFLPRK FPSLRAVPAY GRFIQERFER CLDLYLCPRQ RKMRVNVDPE DLIPKL PRP RDLQPFPTCQ ALVYRGHSDL VRCLSVSPGG QWLVSGSDDG SLRLWEVATA RCVRTVPVGG VVKSVAWNPS PAVCLVA AA VEDSVLLLNP ALGDRLVAGS TDQLLSAFVP PEEPPLQPAR WLEASEEERQ VGLRLRICHG KPVTQVTWHG RGDYLAVV L ATQGHTQVLI HQLSRRRSQS PFRRSHGQVQ RVAFHPARPF LLVASQRSVR LYHLLRQELT KKLMPNCKWV SSLAVHPAG DNVICGSYDS KLVWFDLDLS TKPYRMLRHH KKALRAVAFH PRYPLFASGS DDGSVIVCHG MVYNDLLQNP LLVPVKVLKG HVLTRDLGV LDVIFHPTQP WVFSSGADGT VRLFT

UniProtKB: Ribosome biogenesis protein BOP1

Macromolecule #44: Ribosome biogenesis regulatory protein homolog

• Macromolecule: Name: Ribosome biogenesis regulatory protein homolog / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 41.278523 KDa

Sequence

String:

MEGQSVEELL AKAEQDEAEK LQRITVHKEL ELQFDLGNLL ASDRNPPTGL RCAGPTPEAE LQALARDNTQ LLINQLWQLP TERVEEAIV ARLPEPTTRL PREKPLPRPR PLTRWQQFAR LKGIRPKKKT NLVWDEVSGQ WRRRWGYQRA RDDTKEWLIE V PGNADPLE DQFAKRIQAK KERVAKNELN RLRNLARAHK MQLPSAAGLH PTGHQSKEEL GRAMQVAKVS TASVGRFQER LP KEKVPRG SGKKRKFQPL FGDFAAEKKN QLELLRVMNS KKPQLDVTRA TNKQMREEDQ EEAAKRRKMS QKGKRKGGRQ GPG GKRKGG PPSQGGKRKG GLGGKMNSGP PGLGGKRKGG QRPGGKRRK

UniProtKB: Ribosome biogenesis regulatory protein homolog

Macromolecule #45: Probable ribosome biogenesis protein RLP24

• Macromolecule: Name: Probable ribosome biogenesis protein RLP24 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 19.666258 KDa

Sequence

String:

MRIEKCYFCS GPIYPGHGMM FVRNDCKVFR FCKSKCHKNF KKKRNPRKVR WTKAFRKAAG KELTVDNSFE FEKRRNEPIK YQRELWNKT IDAMKRVEEI KQKRQAKFIM NRLKKNKELQ KVQDIKEVKQ NIHLIRAPLA GKGKQLEEKM VQQLQEDVDM E DAP

UniProtKB: Probable ribosome biogenesis protein RLP24

Macromolecule #46: ATP-dependent RNA helicase DDX18

• Macromolecule: Name: ATP-dependent RNA helicase DDX18 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA helicase
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 75.526789 KDa

Sequence

String:

MSHLPMKLLR KKIEKRNLKL RQRNLKFQGA SNLTLSETQN GDVSEETMGS RKVKKSKQKP MNVGLSETQN GGMSQEAVGN IKVTKSPQK STVLTNGEAA MQSSNSESKK KKKKKRKMVN DAEPDTKKAK TENKGKSEEE SAETTKETEN NVEKPDNDED E SEVPSLPL GLTGAFEDTS FASLCNLVNE NTLKAIKEMG FTNMTEIQHK SIRPLLEGRD LLAAAKTGSG KTLAFLIPAV EL IVKLRFM PRNGTGVLIL SPTRELAMQT FGVLKELMTH HVHTYGLIMG GSNRSAEAQK LGNGINIIVA TPGRLLDHMQ NTP GFMYKN LQCLVIDEAD RILDVGFEEE LKQIIKLLPT RRQTMLFSAT QTRKVEDLAR ISLKKEPLYV GVDDDKANAT VDGL EQGYV VCPSEKRFLL LFTFLKKNRK KKLMVFFSSC MSVKYHYELL NYIDLPVLAI HGKQKQNKRT TTFFQFCNAD SGTLL CTDV AARGLDIPEV DWIVQYDPPD DPKEYIHRVG RTARGLNGRG HALLILRPEE LGFLRYLKQS KVPLSEFDFS WSKISD IQS QLEKLIEKNY FLHKSAQEAY KSYIRAYDSH SLKQIFNVNN LNLPQVALSF GFKVPPFVDL NVNSNEGKQK KRGGGGG FG YQKTKKVEKS KIFKHISKKS SDSRQFSH

UniProtKB: ATP-dependent RNA helicase DDX18

Macromolecule #47: Nucleolar protein 16

• Macromolecule: Name: Nucleolar protein 16 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 21.234486 KDa

Sequence

String:

MPKAKGKTRR QKFGYSVNRK RLNRNARRKA APRIECSHIR HAWDHAKSVR QNLAEMGLAV DPNRAVPLRK RKVKAMEVDI EERPKELVR KPYVLNDLEA EASLPEKKGN TLSRDLIDYV RYMVENHGED YKAMARDEKN YYQDTPKQIR SKINVYKRFY P AEWQDFLD SLQKRKMEVE

UniProtKB: Nucleolar protein 16

Macromolecule #2: 5.8S rRNA

• Macromolecule: Name: 5.8S rRNA / type: rna / ID: 2 / Number of copies: 1
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 50.449812 KDa

Sequence

String:

CGACUCUUAG CGGUGGAUCA CUCGGCUCGU GCGUCGAUGA AGAACGCAGC UAGCUGCGAG AAUUAAUGUG AAUUGCAGGA CACAUUGAU CAUCGACACU UCGAACGCAC UUGCGGCCCC GGGUUCCUCC CGGGGCUACG CCUGUCUGAG CGUCGCUU

GENBANK: GENBANK: U13369.1

Macromolecule #3: ITS2 rRNA

• Macromolecule: Name: ITS2 rRNA / type: rna / ID: 3 / Number of copies: 1
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 376.425312 KDa

Sequence

String:

GCCGAUCAAU CGCCCCCGGG GGUGCCUCCG GGCUCCUCGG GGUGCGCGGC UGGGGGUUCC CUCGCAGGGC CCGCCGGGGG CCCUCCGUC CCCCUAAGCG CAGACCCGGC GGCGUCCGCC CUCCUCUUGC CGCCGCGCCC GCCCCUUCCC CCUCCCCCCG C GGGCCCUG CGUGGUCACG CGUCGGGUGG CGGGGGGGAG AGGGGGGCGC GCCCGGCUGA GAGAGACGGG GAGGGCGGCG CC GCCGCCG CCCGCGAAGA CGGAGAGGGA AAGAGAGAGC CGGCUCGGGC CGAGUUCCCG UGGCCGCCGC CUGCGGUCCG GGU UCCUCC CUCGGGGGGC UCCCUCGCGC CGCGCGCGGC UCGGGGUUCG GGGUUCGUCG GCCCCGGCCG GGUGGAAGGU CCCG UGCCC GUCGUCGUCG UCGUCGUCGC GCGUCGUCGG CGGUGGGGGC GUGUUGCGUG CGGUGUGGUG GUGGGGGAGG AGGAA GGCG GGUCCGGAAG GGGAAGGGUG CCGGCGGGGA GAGAGGGUCG GGGGAGCGCG UCCCGGUCGC CGCGGUUCGC CGCCCG CCC CCGGUGGCGG CCCGGCGUCC GGCCGACCGC CGCUCCCGCG CCCCUCCUCC UCCCCGCCGC CCCUCCUCCG AGGCCCC GC CCGUCCUCCU CGCCCUCCCC GCGCGUACGC GCGCCCGCCC GCCCGGCUCG CCUCGCGGCG CGUCGGCCGG GGCCGGGA G CCCGCCCCGC GGCCCGCCCG GCCGCGCCCG UGGCCGCGGC GCCGGGGUUC GCGUGUCCCC GGCGGCGACC CGCGGGACG CCGCGGUGUC GUCCGCCGUC GCGCGCCCGC CUCCGGCUCG CGGCCGCGCC GCGCCGCGCC GGGGCCCCGU CCCGAGCUUC CGCGUCGGG GCGGGGCGGC UCCGCCGCCG CGUCCUCGGA CCCGUCCCCC CGACCUCCGC GGGGGAGACG GGUCGGGGCG U GCGGCGCC CGUCCCGCCC CCGGCCCGUG CCCCUCCCUC CGGUCGUCCC GCUCCGGCGG GGCGGCGCGG GGGUGCCGCC GG CCGCGCG CUCUCUCUCC CGUCGCCUCU CCCCCUCGCC GGGCCCGUCU CCCGACGGAG CGUCGGGCGG GCGGUCGGGC CGG CGCGAU UCCGUCCGUC CGUCCGCCGA GCGGCCCGUC CCCCUCCGAG A

GENBANK: GENBANK: AL592188.60

Macromolecule #4: 28S rRNA

• Macromolecule: Name: 28S rRNA / type: rna / ID: 4 / Number of copies: 1
• Source (natural): Organism: human (human)
• Molecular weight: Theoretical: 1.640222125 MDa

Sequence

String:

CGCGACCUCA GAUCAGACGU GGCGACCCGC UGAAUUUAAG CAUAUUAGUC AGCGGAGGAG AAGAAACUAA CCAGGAUUCC CUCAGUAAC GGCGAGUGAA CAGGGAAGAG CCCAGCGCCG AAUCCCCGCC CCGCGGCGGG GCGCGGGACA UGUGGCGUAC G GAAGACCC GCUCCCCGGC GCCGCUCGUG GGGGGCCCAA GUCCUUCUGA UCGAGGCCCA GCCCGUGGAC GGUGUGAGGC CG GUAGCGG CCCCCGGCGC GCCGGGCCCG GGUCUUCCCG GAGUCGGGUU GCUUGGGAAU GCAGCCCAAA GCGGGUGGUA AAC UCCAUC UAAGGCUAAA UACCGGCACG AGACCGAUAG UCAACAAGUA CCGUAAGGGA AAGUUGAAAA GAACUUUGAA GAGA GAGUU CAAGAGGGCG UGAAACCGUU AAGAGGUAAA CGGGUGGGGU CCGCGCAGUC CGCCCGGAGG AUUCAACCCG GCGGC GGGU CCGGCCGUGU CGGCGGCCCG GCGGAUCUUU CCCGCCCCCC GUUCCUCCCG ACCCCUCCAC CCGCCCUCCC UUCCCC CGC CGCCCCUCCU CCUCCUCCCC GGAGGGGGCG GGCUCCGGCG GGUGCGGGGG UGGGCGGGCG GGGCCGGGGG UGGGGUC GG CGGGGGACCG UCCCCCGACC GGCGACCGGC CGCCGCCGGG CGCAUUUCCA CCGCGGCGGU GCGCCGCGAC CGGCUCCG G GACGGCUGGG AAGGCCCGGC GGGGAAGGUG GCUCGGGGGG CCCCGUCCGU CCGUCCGUCC GUCCUCCUCC UCCCCCGUC UCCGCCCCCC GGCCCCGCGU CCUCCCUCGG GAGGGCGCGC GGGUCGGGGC GGCGGCGGCG GCGGCGGUGG CGGCGGCGGC GGCGGCGGC GGGACCGAAA CCCCCCCCGA GUGUUACAGC CCCCCCGGCA GCAGCACUCG CCGAAUCCCG GGGCCGAGGG A GCGAGACC CGUCGCCGCG CUCUCCCCCC UCCCGGCGCC CACCCCCGCG GGGAAUCCCC CGCGAGGGGG GUCUCCCCCG CG GGGGCGC GCCGGCGUCU CCUCGUGGGG GGGCCGGGCC ACCCCUCCCA CGGCGCGACC GCUCUCCCAC CCCUCCUCCC CGC GCCCCC GCCCCGGCGA CGGGGGGGGU GCCGCGCGCG GGUCGGGGGG CGGGGCGGAC UGUCCCCAGU GCGCCCCGGG CGGG UCGCG CCGUCGGGCC CGGGGGAGGU UCUCUCGGGG CCACGCGCGC GUCCCCCGAA GAGGGGGACG GCGGAGCGAG CGCAC GGGG UCGGCGGCGA CGUCGGCUAC CCACCCGACC CGUCUUGAAA CACGGACCAA GGAGUCUAAC ACGUGCGCGA GUCGGG GGC UCGCACGAAA GCCGCCGUGG CGCAAUGAAG GUGAAGGCCG GCGCGCUCGC CGGCCGAGGU GGGAUCCCGA GGCCUCU CC AGUCCGCCGA GGGCGCACCA CCGGCCCGUC UCGCCCGCCG CGCCGGGGAG GUGGAGCACG AGCGCACGUG UUAGGACC C GAAAGAUGGU GAACUAUGCC UGGGCAGGGC GAAGCCAGAG GAAACUCUGG UGGAGGUCCG UAGCGGUCCU GACGUGCAA AUCGGUCGUC CGACCUGGGU AUAGGGGCGA AAGACUAAUC GAACCAUCUA GUAGCUGGUU CCCUCCGAAG UUUCCCUCAG GAUAGCUGG CGCUCUCGCA GACCCGACGC ACCCCCGCCA CGCAGUUUUA UCCGGUAAAG CGAAUGAUUA GAGGUCUUGG G GCCGAAAC GAUCUCAACC UAUUCUCAAA CUUUAAAUGG GUAAGAAGCC CGGCUCGCUG GCGUGGAGCC GGGCGUGGAA UG CGAGUGC CUAGUGGGCC ACUUUUGGUA AGCAGAACUG GCGCUGCGGG AUGAACCGAA CGCCGGGUUA AGGCGCCCGA UGC CGACGC UCAUCAGACC CCAGAAAAGG UGUUGGUUGA UAUAGACAGC AGGACGGUGG CCAUGGAAGU CGGAAUCCGC UAAG GAGUG UGUAACAACU CACCUGCCGA AUCAACUAGC CCUGAAAAUG GAUGGCGCUG GAGCGUCGGG CCCAUACCCG GCCGU CGCC GGCAGUCGAG AGUGGACGGG AGCGGCGGGG GCGGCGCGCG CGCGCGCGCG UGUGGUGUGC GUCGGAGGGC GGCGGC GGC GGCGGCGGCG GGGGUGUGGG GUCCUUCCCC CGCCCCCCCC CCCACGCCUC CUCCCCUCCU CCCGCCCACG CCCCGCU CC CCGCCCCCGG AGCCCCGCGG ACGCUACGCC GCGACGAGUA GGAGGGCCGC UGCGGUGAGC CUUGAAGCCU AGGGCGCG G GCCCGGGUGG AGCCGCCGCA GGUGCAGAUC UUGGUGGUAG UAGCAAAUAU UCAAACGAGA ACUUUGAAGG CCGAAGUGG AGAAGGGUUC CAUGUGAACA GCAGUUGAAC AUGGGUCAGU CGGUCCUGAG AGAUGGGCGA GCGCCGUUCC GAAGGGACGG GCGAUGGCC UCCGUUGCCC UCGGCCGAUC GAAAGGGAGU CGGGUUCAGA UCCCCGAAUC CGGAGUGGCG GAGAUGGGCG C CGCGAGGC GUCCAGUGCG GUAACGCGAC CGAUCCCGGA GAAGCCGGCG GGAGCCCCGG GGAGAGUUCU CUUUUCUUUG UG AAGGGCA GGGCGCCCUG GAAUGGGUUC GCCCCGAGAG AGGGGCCCGU GCCUUGGAAA GCGUCGCGGU UCCGGCGGCG UCC GGUGAG CUCUCGCUGG CCCUUGAAAA UCCGGGGGAG AGGGUGUAAA UCUCGCGCCG GGCCGUACCC AUAUCCGCAG CAGG UCUCC AAGGUGAACA GCCUCUGGCA UGUUGGAACA AUGUAGGUAA GGGAAGUCGG CAAGCCGGAU CCGUAACUUC GGGAU AAGG AUUGGCUCUA AGGGCUGGGU CGGUCGGGCU GGGGCGCGAA GCGGGGCUGG GCGCGCGCCG CGGCUGGACG AGGCGC CGC CGCCCCCCCC ACGCCCGGGG CACCCCCCUC GCGGCCCUCC CCCGCCCCAC CCCGCGCGCG CCGCUCGCUC CCUCCCC GC CCCGCGCCCU CUCUCUCUCU CUCUCCCCCG CUCCCCGUCC UCCCCCCUCC CCGGGGGAGC GCCGCGUGGG GGCGGCGG C GGGGGGAGAA GGGUCGGGGC GGCAGGGGCC GGCGGCGGCC CGCCGCGGGG CCCCGGCGGC GGGGGCACGG UCCCCCGCG AGGGGGGCCC GGGCACCCGG GGGGCCGGCG GCGGCGGCGA CUCUGGACGC GAGCCGGGCC CUUCCCGUGG AUCGCCCCAG CUGCGGCGG GCGUCGCGGC CGCCCCCGGG GAGCCCGGCG GGCGCCGGCG CGCCCCCCCC CCCACCCCAC GUCUCGUCGC G CGCGCGUC CGCUGGGGGC GGGGAGCGGU CGGGCGGCGG CGGUCGGCGG GCGGCGGGGC GGGGCGGUUC GUCCCCCCGC CC UACCCCC CCGGCCCCGU CCGCCCCCCG UUCCCCCCUC CUCCUCGGCG CGCGGCGGCG GCGGCGGCAG GCGGCGGAGG GGC CGCGGG CCGGUCCCCC CCGCCGGGUC CGCCCCCGGG GCCGCGGUUC CGCGCGGCGC CUCGCCUCGG CCGGCGCCUA GCAG CCGAC UUAGAACUGG UGCGGACCAG GGGAAUCCGA CUGUUUAAUU AAAACAAAGC AUCGCGAAGG CCCGCGGCGG GUGUU GACG CGAUGUGAUU UCUGCCCAGU GCUCUGAAUG UCAAAGUGAA GAAAUUCAAU GAAGCGCGGG UAAACGGCGG GAGUAA CUA UGACUCUCUU AAGGUAGCCA AAUGCCUCGU CAUCUAAUUA GUGACGCGCA UGAAUGGAUG AACGAGAUUC CCACUGU CC CUACCUACUA UCCAGCGAAA CCACAGCCAA GGGAACGGGC UUGGCGGAAU CAGCGGGGAA AGAAGACCCU GUUGAGCU U GACUCUAGUC UGGCACGGUG AAGAGACAUG AGAGGUGUAG AAUAAGUGGG AGGCCCCCGG CGCCCCCCCG GUGUCCCCG CGAGGGGCCC GGGGCGGGGU CCGCCGGCCC UGCGGGCCGC CGGUGAAAUA CCACUACUCU GAUCGUUUUU UCACUGACCC GGUGAGGCG GGGGGGCGAG CCCCGAGGGG CUCUCGCUUC UGGCGCCAAG CGCCCGGCCG CGCGCCGGCC GGGCGCGACC C GCUCCGGG GACAGUGCCA GGUGGGGAGU UUGACUGGGG CGGUACACCU GUCAAACGGU AACGCAGGUG UCCUAAGGCG AG CUCAGGG AGGACAGAAA CCUCCCGUGG AGCAGAAGGG CAAAAGCUCG CUUGAUCUUG AUUUUCAGUA CGAAUACAGA CCG UGAAAG CGGGGCCUCA CGAUCCUUCU GACCUUUUGG GUUUUAAGCA GGAGGUGUCA GAAAAGUUAC CACAGGGAUA ACUG GCUUG UGGCGGCCAA GCGUUCAUAG CGACGUCGCU UUUUGAUCCU UCGAUGUCGG CUCUUCCUAU CAUUGUGAAG CAGAA UUCA CCAAGCGUUG GAUUGUUCAC CCACUAAUAG GGAACGUGAG CUGGGUUUAG ACCGUCGUGA GACAGGUUAG UUUUAC CCU ACUGAUGAUG UGUUGUUGCC AUGGUAAUCC UGCUCAGUAC GAGAGGAACC GCAGGUUCAG ACAUUUGGUG UAUGUGC UU GGCUGAGGAG CCAAUGGGGC GAAGCUACCA UCUGUGGGAU UAUGACUGAA CGCCUCUAAG UCAGAAUCCC GCCCAGGC G GAACGAUACG GCAGCGCCGC GGAGCCUCGG UUGGCCUCGG AUAGCCGGUC CCCCGCCUGU CCCCGCCGGC GGGCCGCCC CCCCCCUCCA CGCGCCCCGC GCGCGCGGGA GGGCGCGUGC CCCGCCGCGC GCCGGGACCG GGGUCCGGUG CGGAGUGCCC UUCGUCCUG GGAAACGGGG CGCGGCCGGA GAGGCGGCCG CCCCCUCGCC CGUCACGCAC CGCACGUUCG UGGGGAACCU G GCGCUAAA CCAUUCGUAG ACGACCUGCU UCUGGGUCGG GGUUUCGUAC GUAGCAGAGC AGCUCCCUCG CUGCGAUCUA UU GAAAGUC AGCCCUCGAC ACAAGGGUUU GUC

GENBANK: GENBANK: AL592188.60

Macromolecule #48: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 48 / Number of copies: 61 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #49: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 49 / Number of copies: 2 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #50: GUANOSINE-5'-DIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-DIPHOSPHATE / type: ligand / ID: 50 / Number of copies: 1 / Formula: GDP
• Molecular weight: Theoretical: 443.201 Da

Chemical component information

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

Macromolecule #51: POTASSIUM ION

• Macromolecule: Name: POTASSIUM ION / type: ligand / ID: 51 / Number of copies: 1 / Formula: K
• Molecular weight: Theoretical: 39.098 Da

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.6
• Grid: Model: Quantifoil R3.5/1 / Material: GOLD / Mesh: 400 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 2 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 30 sec.
• Vitrification: Cryogen name: ETHANE / Chamber humidity: 95 % / Chamber temperature: 283 K / Instrument: FEI VITROBOT MARK IV; Details: Four applications with manual blotting before last blotting with the vitrobot..

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Specialist optics: Energy filter - Slit width: 20 eV
• Image recording: Film or detector model: GATAN K3 (6k x 4k) / Number grids imaged: 4 / Number real images: 172699 / Average exposure time: 2.0 sec. / Average electron dose: 60.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 0.5 µm / Nominal magnification: 64000
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Particle selection: Number selected: 15679142
• Startup model: Type of model: INSILICO MODEL
• Final reconstruction: Applied symmetry - Point group: C₁ (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 2.88 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 3.3.2) / Number images used: 39938
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 4.0)
• Final angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 3.3.2)
• Final 3D classification: Software: (Name: RELION (ver. 4.0), cryoSPARC (ver. 3.3.2))

Atomic model buiding 1

• Refinement: Space: REAL

Output model

PDB-8fks:
Human nucleolar pre-60S ribosomal subunit (State B2)