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Yorodumi- EMDB-27250: Yeast mitochondrial small subunit assembly intermediate (State 2) -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-27250 | |||||||||
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Title | Yeast mitochondrial small subunit assembly intermediate (State 2) | |||||||||
Map data | ||||||||||
Sample |
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Function / homology | Function and homology information mitochondrial small ribosomal subunit assembly / mitochondrial translational initiation / Mitochondrial protein degradation / mitochondrial small ribosomal subunit / mitochondrial ribosome / sporulation resulting in formation of a cellular spore / mitochondrial translation / superoxide dismutase activity / RNA splicing / methyltransferase activity ...mitochondrial small ribosomal subunit assembly / mitochondrial translational initiation / Mitochondrial protein degradation / mitochondrial small ribosomal subunit / mitochondrial ribosome / sporulation resulting in formation of a cellular spore / mitochondrial translation / superoxide dismutase activity / RNA splicing / methyltransferase activity / mRNA processing / ribosomal small subunit biogenesis / small ribosomal subunit rRNA binding / peroxisome / ribosomal small subunit assembly / 4 iron, 4 sulfur cluster binding / small ribosomal subunit / mitochondrial inner membrane / rRNA binding / ribosome / structural constituent of ribosome / translation / mRNA binding / GTP binding / mitochondrion / RNA binding / ATP binding / metal ion binding / cytoplasm Similarity search - Function | |||||||||
Biological species | Saccharomyces cerevisiae (brewer's yeast) / baker's yeast (brewer's yeast) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.13 Å | |||||||||
Authors | Burnside C / Harper NJ / Klinge S | |||||||||
Funding support | United States, 2 items
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Citation | Journal: Nature / Year: 2023 Title: Principles of mitoribosomal small subunit assembly in eukaryotes. Authors: Nathan J Harper / Chloe Burnside / Sebastian Klinge / Abstract: Mitochondrial ribosomes (mitoribosomes) synthesize proteins encoded within the mitochondrial genome that are assembled into oxidative phosphorylation complexes. Thus, mitoribosome biogenesis is ...Mitochondrial ribosomes (mitoribosomes) synthesize proteins encoded within the mitochondrial genome that are assembled into oxidative phosphorylation complexes. Thus, mitoribosome biogenesis is essential for ATP production and cellular metabolism. Here we used cryo-electron microscopy to determine nine structures of native yeast and human mitoribosomal small subunit assembly intermediates, illuminating the mechanistic basis for how GTPases are used to control early steps of decoding centre formation, how initial rRNA folding and processing events are mediated, and how mitoribosomal proteins have active roles during assembly. Furthermore, this series of intermediates from two species with divergent mitoribosomal architecture uncovers both conserved principles and species-specific adaptations that govern the maturation of mitoribosomal small subunits in eukaryotes. By revealing the dynamic interplay between assembly factors, mitoribosomal proteins and rRNA that are required to generate functional subunits, our structural analysis provides a vignette for how molecular complexity and diversity can evolve in large ribonucleoprotein assemblies. | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_27250.map.gz | 227.8 MB | EMDB map data format | |
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Header (meta data) | emd-27250-v30.xml emd-27250.xml | 52.8 KB 52.8 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_27250_fsc.xml | 14.1 KB | Display | FSC data file |
Images | emd_27250.png | 50.6 KB | ||
Masks | emd_27250_msk_1.map | 244.1 MB | Mask map | |
Others | emd_27250_half_map_1.map.gz emd_27250_half_map_2.map.gz | 191.9 MB 192.7 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-27250 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-27250 | HTTPS FTP |
-Validation report
Summary document | emd_27250_validation.pdf.gz | 947.9 KB | Display | EMDB validaton report |
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Full document | emd_27250_full_validation.pdf.gz | 947.4 KB | Display | |
Data in XML | emd_27250_validation.xml.gz | 22.4 KB | Display | |
Data in CIF | emd_27250_validation.cif.gz | 29.4 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-27250 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-27250 | HTTPS FTP |
-Related structure data
Related structure data | 8d8kMC 8cspC 8csqC 8csrC 8cssC 8cstC 8csuC 8d8jC 8d8lC M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_27250.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.057 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
File | emd_27250_msk_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_27250_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_27250_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
+Entire : Yeast mitochondrial small subunit assembly intermediate (State 2)
+Supramolecule #1: Yeast mitochondrial small subunit assembly intermediate (State 2)
+Macromolecule #1: Probable S-adenosyl-L-methionine-dependent RNA methyltransferase ...
+Macromolecule #2: 37S ribosomal protein MRP13, mitochondrial
+Macromolecule #3: Ribosomal protein VAR1, mitochondrial
+Macromolecule #4: Mitochondrial group I intron splicing factor CCM1
+Macromolecule #5: 37S ribosomal protein SWS2, mitochondrial
+Macromolecule #6: 37S ribosomal protein MRP2, mitochondrial
+Macromolecule #7: 37S ribosomal protein S28, mitochondrial
+Macromolecule #8: 37S ribosomal protein S16, mitochondrial
+Macromolecule #9: 37S ribosomal protein S17, mitochondrial
+Macromolecule #10: 37S ribosomal protein RSM18, mitochondrial
+Macromolecule #11: 37S ribosomal protein S19, mitochondrial
+Macromolecule #12: 37S ribosomal protein MRP21, mitochondrial
+Macromolecule #13: 37S ribosomal protein S25, mitochondrial
+Macromolecule #14: 37S ribosomal protein PET123, mitochondrial
+Macromolecule #15: 37S ribosomal protein S23, mitochondrial
+Macromolecule #16: Mitochondrial 37S ribosomal protein S27
+Macromolecule #17: 37S ribosomal protein S24, mitochondrial
+Macromolecule #18: 37S ribosomal protein MRP10, mitochondrial
+Macromolecule #19: 37S ribosomal protein MRP51, mitochondrial
+Macromolecule #20: 37S ribosomal protein MRP4, mitochondrial
+Macromolecule #21: Protein FYV4, mitochondrial
+Macromolecule #22: 37S ribosomal protein S26, mitochondrial
+Macromolecule #23: 37S ribosomal protein NAM9, mitochondrial
+Macromolecule #24: 37S ribosomal protein MRP1, mitochondrial
+Macromolecule #25: 37S ribosomal protein S5, mitochondrial
+Macromolecule #26: 37S ribosomal protein MRP17, mitochondrial
+Macromolecule #27: 37S ribosomal protein S35, mitochondrial
+Macromolecule #28: 37S ribosomal protein S7, mitochondrial
+Macromolecule #29: 37S ribosomal protein S8, mitochondrial
+Macromolecule #30: 37S ribosomal protein S9, mitochondrial
+Macromolecule #32: 37S ribosomal protein S10, mitochondrial
+Macromolecule #33: 37S ribosomal protein S18, mitochondrial
+Macromolecule #34: unknown protein sequence
+Macromolecule #35: MRPS12 isoform 1
+Macromolecule #31: 15S ribosomal RNA
+Macromolecule #36: IRON/SULFUR CLUSTER
+Macromolecule #37: MAGNESIUM ION
+Macromolecule #38: ADENOSINE-5'-TRIPHOSPHATE
+Macromolecule #39: water
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 61.73 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.0 µm / Nominal magnification: 64000 |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |