[English] 日本語
Yorodumi
- EMDB-27250: Yeast mitochondrial small subunit assembly intermediate (State 2) -

+
Open data


ID or keywords:

Loading...

-
Basic information

Entry
Database: EMDB / ID: EMD-27250
TitleYeast mitochondrial small subunit assembly intermediate (State 2)Mitochondrion
Map data
Sample
  • Complex: Yeast mitochondrial small subunit assembly intermediate (State 2)Mitochondrion
    • Protein or peptide: x 34 types
    • RNA: x 1 types
  • Ligand: x 4 types
Function / homology
Function and homology information


mitochondrial translational initiation / mitochondrial small ribosomal subunit / mitochondrial ribosome / mitochondrial translation / sporulation resulting in formation of a cellular spore / superoxide dismutase activity / Transferases; Transferring one-carbon groups; Methyltransferases / methyltransferase activity / ribosomal small subunit biogenesis / small ribosomal subunit rRNA binding ...mitochondrial translational initiation / mitochondrial small ribosomal subunit / mitochondrial ribosome / mitochondrial translation / sporulation resulting in formation of a cellular spore / superoxide dismutase activity / Transferases; Transferring one-carbon groups; Methyltransferases / methyltransferase activity / ribosomal small subunit biogenesis / small ribosomal subunit rRNA binding / ribosomal small subunit assembly / cytosolic small ribosomal subunit / small ribosomal subunit / methylation / mitochondrial inner membrane / rRNA binding / ribosome / structural constituent of ribosome / translation / mRNA binding / GTP binding / mitochondrion / RNA binding / ATP binding / metal ion binding
Similarity search - Function
Ribosomal protein S22, mitochondrial, budding yeast / Ribosomal protein VAR1 / : / Mitochondrial ribosomal protein (VAR1) / Mitochondrial ribosomal protein MRP51, fungi / Mitochondrial ribosomal protein S25 / Ribosomal protein S24, mitochondrial / Ribosomal protein S23, mitochondrial, fungi / Mitochondrial ribosomal protein subunit / Mitochondrial ribosomal protein S25 ...Ribosomal protein S22, mitochondrial, budding yeast / Ribosomal protein VAR1 / : / Mitochondrial ribosomal protein (VAR1) / Mitochondrial ribosomal protein MRP51, fungi / Mitochondrial ribosomal protein S25 / Ribosomal protein S24, mitochondrial / Ribosomal protein S23, mitochondrial, fungi / Mitochondrial ribosomal protein subunit / Mitochondrial ribosomal protein S25 / Ribosomal protein MRP10, mitochondrial / Ribosomal protein S35, mitochondrial / Ribosomal protein Rsm22-like / Mitochondrial small ribosomal subunit Rsm22 / Eukaryotic mitochondrial regulator protein / IGR protein motif / Protein Fyv4 / IGR protein motif / IGR / PPR repeat family / Ribosomal protein S27/S33, mitochondrial / Ribosomal protein S24/S35, mitochondrial / Mitochondrial ribosomal subunit S27 / Ribosomal protein S24/S35, mitochondrial, conserved domain / Mitochondrial ribosomal subunit protein / Pentatricopeptide (PPR) repeat profile. / Ribosomal protein S23/S29, mitochondrial / Mitochondrial ribosomal death-associated protein 3 / Pentatricopeptide repeat / Manganese/iron superoxide dismutase, C-terminal / Manganese/iron superoxide dismutase, C-terminal domain superfamily / Iron/manganese superoxide dismutases, C-terminal domain / Manganese/iron superoxide dismutase, N-terminal domain superfamily / CHCH / CHCH domain / Coiled coil-helix-coiled coil-helix (CHCH) domain profile. / Ribosomal protein S21 / Ribosomal protein S16, conserved site / Ribosomal protein S16 signature. / Ribosomal protein S21 / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal protein S16 / Ribosomal protein S16 / Ribosomal protein S16 domain superfamily / Ribosomal protein S15, bacterial-type / Ribosomal protein S2 signature 2. / Ribosomal protein S6 / Ribosomal protein S6 / Ribosomal protein S6 superfamily / Ribosomal protein S12, bacterial-type / Translation elongation factor EF1B/ribosomal protein S6 / Ribosomal protein S18 / Ribosomal protein S18 / Ribosomal protein S18 superfamily / Ribosomal protein S14, conserved site / Ribosomal protein S15/S19, conserved site / Ribosomal protein S19/S15 / Ribosomal protein S19/S15, superfamily / Ribosomal protein S10 / Ribosomal protein S14 signature. / Ribosomal protein S7, conserved site / Ribosomal protein S19 / Ribosomal protein S2 signature 1. / Ribosomal protein S13, conserved site / Ribosomal protein S2, conserved site / Ribosomal protein S13 / 30s ribosomal protein S13, C-terminal / Ribosomal protein S2 / Ribosomal protein S2, flavodoxin-like domain superfamily / Ribosomal protein S14 / Ribosomal protein S2 / Ribosomal protein S4, conserved site / Ribosomal protein S13/S18 / Ribosomal protein S4/S9 / Ribosomal protein S19 signature. / Ribosomal protein S14p/S29e / Ribosomal protein S8 / Ribosomal protein S8 superfamily / Ribosomal protein S5, N-terminal, conserved site / Ribosomal protein S5 signature. / Ribosomal protein S5 / Ribosomal protein S5, N-terminal / Ribosomal protein S7 signature. / Ribosomal protein S10p/S20e / Ribosomal protein S13-like, H2TH / S5 double stranded RNA-binding domain profile. / Ribosomal protein S5, C-terminal / Ribosomal protein S9, conserved site / Ribosomal protein S5, N-terminal domain / Ribosomal protein S8 / Ribosomal protein S10 domain / Ribosomal protein S10 domain superfamily / Ribosomal protein S5, C-terminal domain / S4 RNA-binding domain / Ribosomal protein S11 / RNA-binding S4 domain / Ribosomal protein S13 signature. / Ribosomal protein S5/S7 / Ribosomal protein S7 domain / Ribosomal protein S7 domain superfamily
Similarity search - Domain/homology
MRPS12 isoform 1 / CCM1 isoform 1 / Small ribosomal subunit protein mS37 / Small ribosomal subunit protein uS3m / Small ribosomal subunit protein mS43 / Small ribosomal subunit protein uS14m / Small ribosomal subunit protein mS27 / Small ribosomal subunit protein mS26 / Small ribosomal subunit protein uS15m / Small ribosomal subunit protein uS4m ...MRPS12 isoform 1 / CCM1 isoform 1 / Small ribosomal subunit protein mS37 / Small ribosomal subunit protein uS3m / Small ribosomal subunit protein mS43 / Small ribosomal subunit protein uS14m / Small ribosomal subunit protein mS27 / Small ribosomal subunit protein mS26 / Small ribosomal subunit protein uS15m / Small ribosomal subunit protein uS4m / Small ribosomal subunit protein bS6m / Small ribosomal subunit protein uS2m / Small ribosomal subunit protein uS5m / Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial / Small ribosomal subunit protein uS9m / Small ribosomal subunit protein bS21m / Small ribosomal subunit protein mS41 / Small ribosomal subunit protein bS18m / Small ribosomal subunit protein mS23 / Small ribosomal subunit protein uS11m / Small ribosomal subunit protein mS42 / Small ribosomal subunit protein uS7m / Small ribosomal subunit protein mS45 / Small ribosomal subunit protein mS33 / Small ribosomal subunit protein uS19m / Small ribosomal subunit protein uS13m / Small ribosomal subunit protein mS29 / Small ribosomal subunit protein bS16m / Small ribosomal subunit protein bS1m / Small ribosomal subunit protein uS10m / Small ribosomal subunit protein uS17m / Small ribosomal subunit protein uS8m / Small ribosomal subunit protein mS35
Similarity search - Component
Biological speciesSaccharomyces cerevisiae (brewer's yeast) / baker's yeast (brewer's yeast)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.13 Å
AuthorsBurnside C / Harper NJ / Klinge S
Funding support United States, 2 items
OrganizationGrant numberCountry
Other private
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)5T32GM136640-02 United States
CitationJournal: Nature / Year: 2023
Title: Principles of mitoribosomal small subunit assembly in eukaryotes.
Authors: Nathan J Harper / Chloe Burnside / Sebastian Klinge /
Abstract: Mitochondrial ribosomes (mitoribosomes) synthesize proteins encoded within the mitochondrial genome that are assembled into oxidative phosphorylation complexes. Thus, mitoribosome biogenesis is ...Mitochondrial ribosomes (mitoribosomes) synthesize proteins encoded within the mitochondrial genome that are assembled into oxidative phosphorylation complexes. Thus, mitoribosome biogenesis is essential for ATP production and cellular metabolism. Here we used cryo-electron microscopy to determine nine structures of native yeast and human mitoribosomal small subunit assembly intermediates, illuminating the mechanistic basis for how GTPases are used to control early steps of decoding centre formation, how initial rRNA folding and processing events are mediated, and how mitoribosomal proteins have active roles during assembly. Furthermore, this series of intermediates from two species with divergent mitoribosomal architecture uncovers both conserved principles and species-specific adaptations that govern the maturation of mitoribosomal small subunits in eukaryotes. By revealing the dynamic interplay between assembly factors, mitoribosomal proteins and rRNA that are required to generate functional subunits, our structural analysis provides a vignette for how molecular complexity and diversity can evolve in large ribonucleoprotein assemblies.
History
DepositionJun 8, 2022-
Header (metadata) releaseDec 21, 2022-
Map releaseDec 21, 2022-
UpdateFeb 15, 2023-
Current statusFeb 15, 2023Processing site: RCSB / Status: Released

-
Structure visualization

Supplemental images

emd_27250.png

Downloads & links

-
Map

FileDownload / File: emd_27250.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.057 Å
Density
Contour LevelBy AUTHOR: 4.5
Minimum - Maximum-11.920732 - 24.069916
Average (Standard dev.)0.010111051 (±1.0480497)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderZYX
Origin000
Dimensions400400400
Spacing400400400
CellA=B=C: 422.80002 Å
α=β=γ: 90.0 °

-
Supplemental data

-
Mask #1

Fileemd_27250_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

-
Half map: #2

Fileemd_27250_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

-
Half map: #1

Fileemd_27250_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

-
Sample components

+
Entire : Yeast mitochondrial small subunit assembly intermediate (State 2)

EntireName: Yeast mitochondrial small subunit assembly intermediate (State 2)Mitochondrion
Components
  • Complex: Yeast mitochondrial small subunit assembly intermediate (State 2)Mitochondrion
    • Protein or peptide: Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial
    • Protein or peptide: 37S ribosomal protein MRP13, mitochondrialRibosome
    • Protein or peptide: Ribosomal protein VAR1, mitochondrialRibosome
    • Protein or peptide: Mitochondrial group I intron splicing factor CCM1
    • Protein or peptide: 37S ribosomal protein SWS2, mitochondrialRibosome
    • Protein or peptide: 37S ribosomal protein MRP2, mitochondrialRibosome
    • Protein or peptide: 37S ribosomal protein S28, mitochondrialRibosome
    • Protein or peptide: 37S ribosomal protein S16, mitochondrialRibosome
    • Protein or peptide: 37S ribosomal protein S17, mitochondrialRibosome
    • Protein or peptide: 37S ribosomal protein RSM18, mitochondrialRibosome
    • Protein or peptide: 37S ribosomal protein S19, mitochondrialRibosome
    • Protein or peptide: 37S ribosomal protein MRP21, mitochondrialRibosome
    • Protein or peptide: 37S ribosomal protein S25, mitochondrialRibosome
    • Protein or peptide: 37S ribosomal protein PET123, mitochondrialRibosome
    • Protein or peptide: 37S ribosomal protein S23, mitochondrialRibosome
    • Protein or peptide: Mitochondrial 37S ribosomal protein S27
    • Protein or peptide: 37S ribosomal protein S24, mitochondrialRibosome
    • Protein or peptide: 37S ribosomal protein MRP10, mitochondrialRibosome
    • Protein or peptide: 37S ribosomal protein MRP51, mitochondrialRibosome
    • Protein or peptide: 37S ribosomal protein MRP4, mitochondrialRibosome
    • Protein or peptide: Protein FYV4, mitochondrial
    • Protein or peptide: 37S ribosomal protein S26, mitochondrialRibosome
    • Protein or peptide: 37S ribosomal protein NAM9, mitochondrialRibosome
    • Protein or peptide: 37S ribosomal protein MRP1, mitochondrialRibosome
    • Protein or peptide: 37S ribosomal protein S5, mitochondrialRibosome
    • Protein or peptide: 37S ribosomal protein MRP17, mitochondrialRibosome
    • Protein or peptide: 37S ribosomal protein S35, mitochondrialRibosome
    • Protein or peptide: 37S ribosomal protein S7, mitochondrialRibosome
    • Protein or peptide: 37S ribosomal protein S8, mitochondrialRibosome
    • Protein or peptide: 37S ribosomal protein S9, mitochondrialRibosome
    • RNA: 15S ribosomal RNA
    • Protein or peptide: 37S ribosomal protein S10, mitochondrialRibosome
    • Protein or peptide: 37S ribosomal protein S18, mitochondrialRibosome
    • Protein or peptide: unknown protein sequence
    • Protein or peptide: MRPS12 isoform 1
  • Ligand: IRON/SULFUR CLUSTERIron–sulfur cluster
  • Ligand: MAGNESIUM ION
  • Ligand: ADENOSINE-5'-TRIPHOSPHATE
  • Ligand: water

+
Supramolecule #1: Yeast mitochondrial small subunit assembly intermediate (State 2)

SupramoleculeName: Yeast mitochondrial small subunit assembly intermediate (State 2)
type: complex / ID: 1 / Chimera: Yes / Parent: 0 / Macromolecule list: #1-#35
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 1.22 MDa

+
Macromolecule #1: Probable S-adenosyl-L-methionine-dependent RNA methyltransferase ...

MacromoleculeName: Probable S-adenosyl-L-methionine-dependent RNA methyltransferase RSM22, mitochondrial
type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
EC number: Transferases; Transferring one-carbon groups; Methyltransferases
Source (natural)Organism: baker's yeast (brewer's yeast)
Molecular weightTheoretical: 72.308328 KDa
SequenceString: MMKRCFSILP QNVRFSSKFT SLNLPKLDLA DFIDSNKRGI NVLPSYRDET ASTTQATNSK ELRLLSKTLQ GQSYRDQLEL NPDVSKAIN NNIMAVHIPN NLRRVATNYY KEIQEPNSLH RPCRTKMEVD AHIASIFLQN YGSIFQSLKE LQKRVGPDNF K PQRILDVG ...String:
MMKRCFSILP QNVRFSSKFT SLNLPKLDLA DFIDSNKRGI NVLPSYRDET ASTTQATNSK ELRLLSKTLQ GQSYRDQLEL NPDVSKAIN NNIMAVHIPN NLRRVATNYY KEIQEPNSLH RPCRTKMEVD AHIASIFLQN YGSIFQSLKE LQKRVGPDNF K PQRILDVG YGPATGIVAL NDILGPNYRP DLKDAVILGN AEMQERAKII LSRQLNEVVD TVEENVSTEK EQETDRRNKN FQ EDEHIGE VMTKKINIMT NLRSSIPASK EYDLIILTHQ LLHDGNQFPI QVDENIEHYL NILAPGGHIV IIERGNPMGF EII ARARQI TLRPENFPDE FGKIPRPWSR GVTVRGKKDA ELGNISSNYF LKVIAPCPHQ RKCPLQVGNP NFYTHKEGKD LKFC NFQKS IKRPKFSIEL KKGKLLATSW DGSQGNASRL KGTGRRNGRD YEILNYSYLI FERSHKDENT LKEIKKLRNE NVNGK YDIG SLGDDTQNSW PRIINDPVKR KGHVMMDLCA PSGELEKWTV SRSFSKQIYH DARKSKKGDL WASAAKTQIK GLGDLN VKK FHKLEKERIK QLKKEERQKA RKAMESYNEL EDSLQFDDHQ FSNFEVMKKL STFHGNDFLQ HVNRK

+
Macromolecule #2: 37S ribosomal protein MRP13, mitochondrial

MacromoleculeName: 37S ribosomal protein MRP13, mitochondrial / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast)
Molecular weightTheoretical: 39.045031 KDa
SequenceString: MGTITVVINE GPILLIRALH RATTNKKMFR STVWRRFAST GEIAKAKLDE FLIYHKTDAK LKPFIYRPKN AQILLTKDIR DPKTREPLQ PRPPVKPLSK QTLNDFIYSV EPNSTELLDW FKEWTGTSIR KRAIWTYISP IHVQKMLTAS FFKIGKYAHM V GLLYGIEH ...String:
MGTITVVINE GPILLIRALH RATTNKKMFR STVWRRFAST GEIAKAKLDE FLIYHKTDAK LKPFIYRPKN AQILLTKDIR DPKTREPLQ PRPPVKPLSK QTLNDFIYSV EPNSTELLDW FKEWTGTSIR KRAIWTYISP IHVQKMLTAS FFKIGKYAHM V GLLYGIEH KFLKAQNPSV FDIEHFFNTN IMCALHRNRL KDYKDAEIAQ RKLQVAWKKV LNRKNNTGLA NILVATLGRQ IG FTPELTG LQPVDISLPD IPNSSSGAEL KDLLSKYEGI YLIARTLLDI DQHNAQYLEL QEFIRQYQNA LSESSDPYDT HLK ALGLLE TPPPQESTEK EEK

+
Macromolecule #3: Ribosomal protein VAR1, mitochondrial

MacromoleculeName: Ribosomal protein VAR1, mitochondrial / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast)
Molecular weightTheoretical: 47.170996 KDa
SequenceString: MKLKLLNMIL SMMNKTNNNN NIIINNTLDS LMNKKLLLKN MLLDMNNKKM NNMKRMLNNN NMNPAGANPV VHRIGPAGNI NNKLQHLNN MNNWNTQIYN YNKNMEIMNT MNDKLINKLL YKMMTLKLNN MNINKIIMSK TINQHSLNKL NIKFYYYNND I NNNNNNNN ...String:
MKLKLLNMIL SMMNKTNNNN NIIINNTLDS LMNKKLLLKN MLLDMNNKKM NNMKRMLNNN NMNPAGANPV VHRIGPAGNI NNKLQHLNN MNNWNTQIYN YNKNMEIMNT MNDKLINKLL YKMMTLKLNN MNINKIIMSK TINQHSLNKL NIKFYYYNND I NNNNNNNN NNYYMNMMNK LMNIMNNNMN NNLCNILSYY YKKKVTIEPI KLSYIYLNSD IFSKYISLND MDKYNNGILT NY QRMLNNI MPKLNDHNIS MNYINNINNI NNNKYNNMIN LLNNNNNINN NNNYNNNNNN YIGNINNIYN NMTIDNIPMD ILM YKYLVG WSIKFKGRLS NNNGRTSTTN LLNGTFNNKK YLWSNINNNY KLNYIPSNHN LYNNSNINKN GKYNIKVKLN FI

+
Macromolecule #4: Mitochondrial group I intron splicing factor CCM1

MacromoleculeName: Mitochondrial group I intron splicing factor CCM1 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast)
Molecular weightTheoretical: 101.568547 KDa
SequenceString: MYMARCGPKN NVLCFPFQLS FLFSKRLINK RFKYTLQTED EKNMMGSLSK NKIITPEDVE FKLAQLREFS NTLKERIHNT KSVNSDGHQ SNSIAPISED SRNVNVTKTS SVPNEEKSKN LSDLIHSSFL EKMDHLVPKV IRERVADDDI LAKNLFDRSH S NWAPVIDR ...String:
MYMARCGPKN NVLCFPFQLS FLFSKRLINK RFKYTLQTED EKNMMGSLSK NKIITPEDVE FKLAQLREFS NTLKERIHNT KSVNSDGHQ SNSIAPISED SRNVNVTKTS SVPNEEKSKN LSDLIHSSFL EKMDHLVPKV IRERVADDDI LAKNLFDRSH S NWAPVIDR LYVSEKRFMD IDSREFSVWL NGTVKYLPFH SILHLDEMLL EQINGDVVKF NTHMYECIFN NLGNLKPTNF NQ DGTNDKV ILKMKELLER YDKALKITEE RINKKEGFPS KVPKMTQAIL NNCLKYSTKC SSFHDMDYFI TKFRDDYGIT PNK QNLTTV IQFYSRKEMT KQAWNTFDTM KFLSTKHFPD ICTYNTMLRI CEKERNFPKA LDLFQEIQDH NIKPTTNTYI MMAR VLASS SSNAVVSEGK SDSLRLLGWK YLHELEDKNL YRHKKDDLNL FLAMMALAAF DGDIELSRAL YYLFIAKKYK TLCAN WKGN ILVDQDTIWK STLMPEMLNY LMLAYARFDP RNLPVLSGYE KGIELRRKFL REFDSSMRLD DTDKLVKFKL PFLPIS DLN SEAQVLAESN AIWSFNMENG GTRNTLTSSN EAALEDIKKY RQLLDSFAQE AEDFNEFKFK VMYEVTKMQR ESINVNV FN KISLHTYLSI PINLKQQKEF LRRLTFFTFQ QHEFEAVIKR LYEGYRNIPS SHTRDQNSIS TEAISVSKPE TTEDLNLI M HDIWYITCLR HKIMMDTTLY ELVMKAAIEF QNEDLAKKVW NDRGKFRTTV PFLKMDQRIR IAKDQKFAHL MVEFFTKQG KYSDAIAIIL SSKNRFNWTY SMVRNLHKAL EEIEDRNSVE ILLDVVNKKS HAKALKWEEQ ELNM

+
Macromolecule #5: 37S ribosomal protein SWS2, mitochondrial

MacromoleculeName: 37S ribosomal protein SWS2, mitochondrial / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast)
Molecular weightTheoretical: 16.121047 KDa
SequenceString:
MVVHILGKGF KGKEVIKIAL ASKFYGIGKT TAEKICSKLG FYPWMRMHQL SEPQIMSIAS ELSTMTIEGD ARAIVKDNIA LKRKIGSYS GMRHTLHLPV RGQHTRNNAK TARKLNKIDR RGIHTFSQAK VQHNPSLWSC IFGK

+
Macromolecule #6: 37S ribosomal protein MRP2, mitochondrial

MacromoleculeName: 37S ribosomal protein MRP2, mitochondrial / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast)
Molecular weightTheoretical: 13.563061 KDa
SequenceString:
MGNFRFPIKT KLPPGFINAR ILRDNFKRQQ FKENEILVKS LKFIARNMNL PTKLRLEAQL KLNALPNYMR STQIKNRCVD SGHARFVLS DFRLCRYQFR ENALKGNLPG VKKGIW

+
Macromolecule #7: 37S ribosomal protein S28, mitochondrial

MacromoleculeName: 37S ribosomal protein S28, mitochondrial / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast)
Molecular weightTheoretical: 33.116383 KDa
SequenceString: MSIVGRNAIL NLRISLCPLF MGKRSFVSSP VSNSAKAVKF LKAQRRKQKN EAKQATLKAS TDKVDPVLGR ADTPFITRIM AELKEPLVL SKGYNIEEVD KFLAAIESAK RERAELSGLN TEVVGIEDIE KLEDRREAIL RILSMRNSEN KNAIKMAVEL A RKEFERFP ...String:
MSIVGRNAIL NLRISLCPLF MGKRSFVSSP VSNSAKAVKF LKAQRRKQKN EAKQATLKAS TDKVDPVLGR ADTPFITRIM AELKEPLVL SKGYNIEEVD KFLAAIESAK RERAELSGLN TEVVGIEDIE KLEDRREAIL RILSMRNSEN KNAIKMAVEL A RKEFERFP GDTGSSEVQA ACMTVRIQNM ANHIKEHRKD FANTRNLRIL VQQRQAILRY LKRDNPEKYY WTIQKLGLND AA ITDEFNM DRRYMQDYEF FGDKILIRDS KKVANQKRKE IRKQKRATF

+
Macromolecule #8: 37S ribosomal protein S16, mitochondrial

MacromoleculeName: 37S ribosomal protein S16, mitochondrial / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast)
Molecular weightTheoretical: 13.663011 KDa
SequenceString:
MTCGLVRIRL ARFGRKNSPV YNIVVANSRK ARDAKPIEVL GTYVPVPSPV TKRELKRGVV PIKDVKLDFD RTKYWIGVGA QPSETVTKL LRKAGILNDA WATSKNSNVN RKVVFERMET LE

+
Macromolecule #9: 37S ribosomal protein S17, mitochondrial

MacromoleculeName: 37S ribosomal protein S17, mitochondrial / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast)
Molecular weightTheoretical: 27.683986 KDa
SequenceString: MARQNFLGLV VSQGKMQKTV KVRVETKVFN KKINKELFHR RDYLVHDEGE ISREGDLVRI EATRPLSKRK FFAIAEIIRN KGQQFALYE SEAQLSVAKE EAQKAKEFLD KRSVRENKLN EKTTLLRDIR TIQDALSSGS TPKELLEIKQ RYGIQDFSQE T VRQLLQLD ...String:
MARQNFLGLV VSQGKMQKTV KVRVETKVFN KKINKELFHR RDYLVHDEGE ISREGDLVRI EATRPLSKRK FFAIAEIIRN KGQQFALYE SEAQLSVAKE EAQKAKEFLD KRSVRENKLN EKTTLLRDIR TIQDALSSGS TPKELLEIKQ RYGIQDFSQE T VRQLLQLD ISGLEVNLEK QRSLIDRIQT RLSELLSNDL KCDQFLKDHG VEDPLTLKKN IKKNLLRKHV MMDMQQPSQ

+
Macromolecule #10: 37S ribosomal protein RSM18, mitochondrial

MacromoleculeName: 37S ribosomal protein RSM18, mitochondrial / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast)
Molecular weightTheoretical: 15.86649 KDa
SequenceString:
MQPIIKGAVS STFKRALYNF GIKEKKSVNI EMGRTQQTKK IDQSLSKKLP KGTIYDPFDF SMGRIHLDRK YQANKNSNRN DIMKSGANP LEFYARPRIL SRYVTSTGRI QHRDITGLSA KNQRRLSKAI RRCQAIGLM

+
Macromolecule #11: 37S ribosomal protein S19, mitochondrial

MacromoleculeName: 37S ribosomal protein S19, mitochondrial / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast)
Molecular weightTheoretical: 10.292044 KDa
SequenceString:
MQPAARLLSR SVWKGPNIVP LPIREAMTKG TPIRTNARAA TILPQFVGLK FQIHNGKEYV PIEISEDMVG HKLGEFAPTR KRFSYTQTK NK

+
Macromolecule #12: 37S ribosomal protein MRP21, mitochondrial

MacromoleculeName: 37S ribosomal protein MRP21, mitochondrial / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast)
Molecular weightTheoretical: 20.432744 KDa
SequenceString:
MLKSTLRLSR ISLRRGFTTI DCLRQQNSDI DKIILNPIKL AQGSNSDRGQ TSKSKTDNAD ILSMEIPVDM MQSAGRINKR ELLSEAEIA RSSVENAQMR FNSGKSIIVN KNNPAESFKR LNRIMFENNI PGDKRSQRFY MKPGKVAELK RSQRHRKEFM M GFKRLIEI VKDAKRKGY

+
Macromolecule #13: 37S ribosomal protein S25, mitochondrial

MacromoleculeName: 37S ribosomal protein S25, mitochondrial / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast)
Molecular weightTheoretical: 30.554354 KDa
SequenceString: MKIQTNAVNV LQRTSAYLKS GLLKETPAWY NVVASIPPST KFTREPRFKN PSNGHIIGKL VDVTEQPHAN NKGLYKTRPN SSDKRVGVK RLYRPPKLTY VEDRLRSLFY KQHPWELSRP KILVENEIGD ENYDWSHMLQ IGRPLDGESV IQRTMYLIKT K QYGDMVEA ...String:
MKIQTNAVNV LQRTSAYLKS GLLKETPAWY NVVASIPPST KFTREPRFKN PSNGHIIGKL VDVTEQPHAN NKGLYKTRPN SSDKRVGVK RLYRPPKLTY VEDRLRSLFY KQHPWELSRP KILVENEIGD ENYDWSHMLQ IGRPLDGESV IQRTMYLIKT K QYGDMVEA YDHARYEFYA LRMQEETEQQ VALEEAEMFG SLFGVSAIEH GIQKEQEVLD VWEKKVVEET ELMAARTSNP AG SWKDDTT LNTAQEEEST TSENLHF

+
Macromolecule #14: 37S ribosomal protein PET123, mitochondrial

MacromoleculeName: 37S ribosomal protein PET123, mitochondrial / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast)
Molecular weightTheoretical: 36.059281 KDa
SequenceString: MGKGAAKYGF KSGVFPTTRS ILKSPTTKQT DIINKVKSPK PKGVLGIGYA KGVKHPKGSH RLSPKVNFID VDNLIAKTVA EPQSIKSSN GSAQKVRLQK AELRRKFLIE AFRKEEARLL HKHEYLQKRT KELEKAKELE LEKLNKEKSS DLTIMTLDKM M SQPLLRNR ...String:
MGKGAAKYGF KSGVFPTTRS ILKSPTTKQT DIINKVKSPK PKGVLGIGYA KGVKHPKGSH RLSPKVNFID VDNLIAKTVA EPQSIKSSN GSAQKVRLQK AELRRKFLIE AFRKEEARLL HKHEYLQKRT KELEKAKELE LEKLNKEKSS DLTIMTLDKM M SQPLLRNR SPEESELLKL KRNYNRSLLN FQAHKKKLNE LLNLYHVANE FIVTESQLLK KIDKVFNDET EEFTDAYDVT SN FTQFGNR KLLLSGNTTL QTQINNAIMG SLSNEKFFDI SLVDSYLNKD LKNISNKIDS KLNPTSNGAG NNGNNNNTTN L

+
Macromolecule #15: 37S ribosomal protein S23, mitochondrial

MacromoleculeName: 37S ribosomal protein S23, mitochondrial / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast)
Molecular weightTheoretical: 50.940742 KDa
SequenceString: MLRMSTSRFI GQRLFTTARS LQAAKPAPKG KTQGFSKKSS SVSSYSSAKR VTPGSLYKNW TNTTHTAQLQ QTAVPLALPI FNFDDISKT LNKVVSYSNK QYKSLHHLGS FKKSQFNELF QKPVCLVRED ATNSFLKKLV SHPVKKFIIT GEPGVGKTVL L SQAHAYAV ...String:
MLRMSTSRFI GQRLFTTARS LQAAKPAPKG KTQGFSKKSS SVSSYSSAKR VTPGSLYKNW TNTTHTAQLQ QTAVPLALPI FNFDDISKT LNKVVSYSNK QYKSLHHLGS FKKSQFNELF QKPVCLVRED ATNSFLKKLV SHPVKKFIIT GEPGVGKTVL L SQAHAYAV DSKQIIINIS YPELFLNGRN DFSYDDDLKL FIQPMYLKKL IRKILKANDP ALLKSIELSK DYKFSNANPK NA SVKPFVT LNKTKNTVLD LLSVMTHPHN RGKLMKAIID ELSVQSKVPI MFTVDNFSKV LTTAYSAYRN TENKQIYSLD LQM GKLMMD IISGETKFAN GESSTILAIS GVDRTNKTLP VALGKIPVDP YVTRYHYEPK FVELLQKGNV TEFEVPKLNK QEVN ELIDY YKQSNVLLDK DITGKKWENL IDEKYFLSGN GNPRELLKSL VLSHR

+
Macromolecule #16: Mitochondrial 37S ribosomal protein S27

MacromoleculeName: Mitochondrial 37S ribosomal protein S27 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast)
Molecular weightTheoretical: 12.420666 KDa
SequenceString:
MNVPKARLLK VAELSAKIFD QNFNPSGIRT GSKILNERLK GPSVASYYGN PDILKFRHLK TLYPDIEFVD LEEQYRLSMV EAKKRRGKG APKKMKKDAA ATAKGKGKKK K

+
Macromolecule #17: 37S ribosomal protein S24, mitochondrial

MacromoleculeName: 37S ribosomal protein S24, mitochondrial / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast)
Molecular weightTheoretical: 37.458496 KDa
SequenceString: MKVPLGLWKV SRGNLWSTQK RVLTMSRCLN SDAGNEAKTV REGPAFSADL YMHPEKWKGL PPQRILELYW ERMARLGSEY KPNKDELNA LLTTSEYSNV PVNDIKKLYH RGEQGAIDIK GGNVNRDNSL RPFMFDELPS QAQELVAQHR EQRFYNRLAA Y ELPLLAQY ...String:
MKVPLGLWKV SRGNLWSTQK RVLTMSRCLN SDAGNEAKTV REGPAFSADL YMHPEKWKGL PPQRILELYW ERMARLGSEY KPNKDELNA LLTTSEYSNV PVNDIKKLYH RGEQGAIDIK GGNVNRDNSL RPFMFDELPS QAQELVAQHR EQRFYNRLAA Y ELPLLAQY RQEYKRPSPE SHPVTYRYTS YVGEEHPNSR KVVLSVKTKE LGLEEKSLHK FRILARSRYD HTTDIFKMSS DK FEHASQN ARYLHDILQR LLAESKDLTE DDFSDVPLDT RHTIAKSLRK KKRDYEFPEH WKRPEDAPKK KFDIVDQLLS TL

+
Macromolecule #18: 37S ribosomal protein MRP10, mitochondrial

MacromoleculeName: 37S ribosomal protein MRP10, mitochondrial / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast)
Molecular weightTheoretical: 10.709598 KDa
SequenceString:
MSGKPPVYRL PPLPRLKVKK PIIRQEANKC LVLMSNLLQC WSSYGHMSPK CAGLVTELKS CTSESALGKR NNVQKSNINY HAARLYDRI NGKPHD

+
Macromolecule #19: 37S ribosomal protein MRP51, mitochondrial

MacromoleculeName: 37S ribosomal protein MRP51, mitochondrial / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast)
Molecular weightTheoretical: 39.507457 KDa
SequenceString: MTLAELLGRS RIAQVANNHK PLTYTGKKFH PTHQIIETKP STLYRQEWGL KSAIPSKIKS RYLVYNDLDT LERITTFEPR GGTQWNRLR FQEMGVPIVS NIGRQNPFFK YISRPEDESH AKLSLFKEMK GDTDISPAAM KKRLKKITAL IRSFQDEFKE W LVENHPDE ...String:
MTLAELLGRS RIAQVANNHK PLTYTGKKFH PTHQIIETKP STLYRQEWGL KSAIPSKIKS RYLVYNDLDT LERITTFEPR GGTQWNRLR FQEMGVPIVS NIGRQNPFFK YISRPEDESH AKLSLFKEMK GDTDISPAAM KKRLKKITAL IRSFQDEFKE W LVENHPDE LKLNSNKLED YVVKFLNKKL ETKTNKKFNT EIIGTGGLSY SLPGKLKNSP NGVIQRTVVP GRILNVVKEN ND NKWLAAI GGFVADVVFF QSPPSSFNSM GDFIRMKTFL FEILEASMEK NGSVSMHARL LEPQNDKTRE FFNKRPIYKP LTS RRARRP SVGNIQEANN LLNIIKGN

+
Macromolecule #20: 37S ribosomal protein MRP4, mitochondrial

MacromoleculeName: 37S ribosomal protein MRP4, mitochondrial / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast)
Molecular weightTheoretical: 44.205727 KDa
SequenceString: MQRHVFARNF RRLSLLRNPS LTKRFQSSAS GAANTPNNND EVMLLQQKLL YDEIRSELKS LSQVPEDEIL PELKKSLEQD KLSDKEQQL EAELSDFFRN YALLNKLFDS KTLDGQSSTT TAAATPTKPY PNLIPSANDK PYSSQELFLR QLNHSMRTAK L GATISKVY ...String:
MQRHVFARNF RRLSLLRNPS LTKRFQSSAS GAANTPNNND EVMLLQQKLL YDEIRSELKS LSQVPEDEIL PELKKSLEQD KLSDKEQQL EAELSDFFRN YALLNKLFDS KTLDGQSSTT TAAATPTKPY PNLIPSANDK PYSSQELFLR QLNHSMRTAK L GATISKVY YPHKDIFYPP LPENITVESL MSAGVHLGQS TSLWRSSTQS YIYGEYKGIH IIDLNQTLSY LKRAAKVVEG VS ESGGIIL FLGTRQGQKR GLEEAAKKTH GYYVSTRWIP GTLTNSTEIS GIWEKQEIDS NDNPTERALS PNETSKQVKP DLL VVLNPT ENRNALLEAI KSRVPTIAII DTDSEPSLVT YPIPGNDDSL RSVNFLLGVL ARAGQRGLQN RLARNNEK

+
Macromolecule #21: Protein FYV4, mitochondrial

MacromoleculeName: Protein FYV4, mitochondrial / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast)
Molecular weightTheoretical: 15.321684 KDa
SequenceString:
MIPSRISHKF PLFLRSSLAA PKAAYRFSST IPKPSDQVPD VDAFLNKIGR NCNELKDTFE NNWNNLFQWD SKILKEKGVN IQQRKYILK QVHNYRNNRP IHEIKLGKKS FFGGERKRKA FTAKWKAENK Q

+
Macromolecule #22: 37S ribosomal protein S26, mitochondrial

MacromoleculeName: 37S ribosomal protein S26, mitochondrial / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast)
Molecular weightTheoretical: 30.257449 KDa
SequenceString: MLVFKRGIHV VPKLPNSKAL LQNGVPNILS SSGFKTVWFD YQRYLCDKLT LATAGQSLES YYPFHILLKT AGNPLQSNIF NLASSIHNN HLFVENILPS AVEHGTNSNA VVKTEPSRLF LSKIKDSFNG SDWEVVKEEM IYRAENEVLG QGWLFLVENN E KKLFILTS ...String:
MLVFKRGIHV VPKLPNSKAL LQNGVPNILS SSGFKTVWFD YQRYLCDKLT LATAGQSLES YYPFHILLKT AGNPLQSNIF NLASSIHNN HLFVENILPS AVEHGTNSNA VVKTEPSRLF LSKIKDSFNG SDWEVVKEEM IYRAENEVLG QGWLFLVENN E KKLFILTS NNNGTPYYFP RNQSFDLNSA ISIDEFATLK QMKELIGKST KLNGKVQDWT MPIICVNLWD HAYLHDYGVG NR SKYVKNV LDNLNWSVVN NRIFSGISK

+
Macromolecule #23: 37S ribosomal protein NAM9, mitochondrial

MacromoleculeName: 37S ribosomal protein NAM9, mitochondrial / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast)
Molecular weightTheoretical: 56.450949 KDa
SequenceString: MPRKANLLKS LARGRVRTSF NKYNLFNLYK KGGVDLKSKS LYQQKWTAKQ ETRAYHGEHL TEKRWQTVFK PKLDSVAQLD ASLRGGEIK ETPFLLQTFA VLEKRLDFAL FRAMFASSVR QARQFILHGN VRVNGVKIKH PSYTLKPGDM FSVKPDKVLE A LGAKKPSF ...String:
MPRKANLLKS LARGRVRTSF NKYNLFNLYK KGGVDLKSKS LYQQKWTAKQ ETRAYHGEHL TEKRWQTVFK PKLDSVAQLD ASLRGGEIK ETPFLLQTFA VLEKRLDFAL FRAMFASSVR QARQFILHGN VRVNGVKIKH PSYTLKPGDM FSVKPDKVLE A LGAKKPSF QEALKIDKTQ IVLWNKYVKE AKTEPKEVWE KKLENFEKMS DSNPKKLQFQ EFLRQYNKNL ESQQYNALKG CT QEGILRK LLNVEKEIGK SNNEPLSIDE LKQGLPEIQD SQLLESLNNA YQEFFKSGEI RREIISKCQP DELISLATEM MNP NETTKK ELSDGAKSAL RSGKRIIAES VKLWTKNITD HFKTRMSDIS DGSLTFDPKW AKNLKYHDPI KLSELEGDEP KARK LINLP WQKNYVYGRQ DPKKPFFTPW KPRPFLSPFA ILPHHLEISF KTCHAVYLRD PVARPGQSEV ISPFDVPVHE RAYMY YLRN GK

+
Macromolecule #24: 37S ribosomal protein MRP1, mitochondrial

MacromoleculeName: 37S ribosomal protein MRP1, mitochondrial / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast)
Molecular weightTheoretical: 36.775781 KDa
SequenceString: MLRFTGARAI RKYSTRYALE HLKEGAPLKG LFSIEGLQKA WFDRVKYLDA KLNDCTNEAQ QKPLETLIHE NSKSASKKHI VNYASSLYN LKFSMSSLQG CIRTPPEECP RLGPEALLQT PDFNRTISNE PLTTGNERLQ AALISSFGSL MEFRTLLINS N LAISGDGF ...String:
MLRFTGARAI RKYSTRYALE HLKEGAPLKG LFSIEGLQKA WFDRVKYLDA KLNDCTNEAQ QKPLETLIHE NSKSASKKHI VNYASSLYN LKFSMSSLQG CIRTPPEECP RLGPEALLQT PDFNRTISNE PLTTGNERLQ AALISSFGSL MEFRTLLINS N LAISGDGF TWLVARRQLD KRAMRNDMPN RDIEYDKLFI LNTYNAGTPF NFSTSGVMNE LNNQYTNMEK QRAKEAGNLE DS EMTAKQA KTKFIYETQQ KGFSGKEVSY IPLLAIDASP KTWLTDYGVF GKREYLERVW DSIEWKIVES RLPQRTKIQA FNT L

+
Macromolecule #25: 37S ribosomal protein S5, mitochondrial

MacromoleculeName: 37S ribosomal protein S5, mitochondrial / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast)
Molecular weightTheoretical: 34.941102 KDa
SequenceString: MFKRQLSTSV RYLQHYDESL LSRYYPESLL KSIKLAQQTI PEDTKFRVSR NVEFAPPYLD DFTKIHPFWD YKPGMPHLHA QEENNNFSI FRWDQVQQPL PGEGNILPPG VSLPNDGGRK SKSADVAAGL HKQTGVDPDY ITRKLTMKPL VMKRVSNQTG K GKIASFYA ...String:
MFKRQLSTSV RYLQHYDESL LSRYYPESLL KSIKLAQQTI PEDTKFRVSR NVEFAPPYLD DFTKIHPFWD YKPGMPHLHA QEENNNFSI FRWDQVQQPL PGEGNILPPG VSLPNDGGRK SKSADVAAGL HKQTGVDPDY ITRKLTMKPL VMKRVSNQTG K GKIASFYA LVVVGDKNGM VGLGEGKSRE EMSKAIFKAH WDAVRNLKEI PRYENRTIYG DIDFRYHGVK LHLRSAKPGF GL RVNHVIF EICECAGIKD LSGKVYKSRN DMNIAKGTIE AFTKAQKTLD EVALGRGKKL VDVRKVYYSS

+
Macromolecule #26: 37S ribosomal protein MRP17, mitochondrial

MacromoleculeName: 37S ribosomal protein MRP17, mitochondrial / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast)
Molecular weightTheoretical: 15.046702 KDa
SequenceString:
MLYELIGLVR ITNSNAPKLE AKELSSTIGK LIIQNRGVVR DIVPMGIRYL PKIMKKDQEK HFRAYHFLML FDSSAAVQSE ILRTLKKDP RVIRSSIVKV DLDKQLDRAS SLHRSLGKKS ILELVNEDYQ SI

+
Macromolecule #27: 37S ribosomal protein S35, mitochondrial

MacromoleculeName: 37S ribosomal protein S35, mitochondrial / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast)
Molecular weightTheoretical: 39.638172 KDa
SequenceString: MSYGLTGTSS KLRGTSSIFS WTQVRHFSRR RIAYPFYPFK KLGRQHPKKH DTNLKTAMRQ FLGPKNYKGE YVMNKYFTVP TNHVPNYIK PDLERGQSLE HPVTKKPLQL RYDGTLGPPP VENKRLQNIF KDRLLQPFPS NPHCKTNYVL SPQLKQSIFE E ITVEGLSA ...String:
MSYGLTGTSS KLRGTSSIFS WTQVRHFSRR RIAYPFYPFK KLGRQHPKKH DTNLKTAMRQ FLGPKNYKGE YVMNKYFTVP TNHVPNYIK PDLERGQSLE HPVTKKPLQL RYDGTLGPPP VENKRLQNIF KDRLLQPFPS NPHCKTNYVL SPQLKQSIFE E ITVEGLSA QQVSQKYGLK IPRVEAIVKL VSVENSWNRR NRVSSDLKTM DETLYRMFPV FDSDASFKRE NLSEIPVPQK TL ASRFLTI AESEPFGPVD AAHVLELEPA VETLRNLSTV GEHSSGHQQS TNKNTKVIYG ELVEGERSQY KFTNAKVGKV GYR YGSGNR DNKKDRRIGF NKLGQMVYI

+
Macromolecule #28: 37S ribosomal protein S7, mitochondrial

MacromoleculeName: 37S ribosomal protein S7, mitochondrial / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast)
Molecular weightTheoretical: 27.857141 KDa
SequenceString: MLHCARRYML VRPRLLWQSG TCVARFQSSV RTPASEPSAE KGVDEWLEAI NELREEFSAK EYLPETSLAP PGQSKVDLLQ GSQAGSKIK PTAEQLAQWE ALKSVPIPPR KNATLDHITN MIMRHGKKEK AQTILSRALY LVYCQTRQDP IQALEKSLDE L APLMMTKT ...String:
MLHCARRYML VRPRLLWQSG TCVARFQSSV RTPASEPSAE KGVDEWLEAI NELREEFSAK EYLPETSLAP PGQSKVDLLQ GSQAGSKIK PTAEQLAQWE ALKSVPIPPR KNATLDHITN MIMRHGKKEK AQTILSRALY LVYCQTRQDP IQALEKSLDE L APLMMTKT FNTGVAKASV IPVPLNKRQR NRIAWNWIVQ SANQRVSSDF AVRLGEELTA IAKGTSSAFE KRDQIHKTAI AH RAYIQLK

+
Macromolecule #29: 37S ribosomal protein S8, mitochondrial

MacromoleculeName: 37S ribosomal protein S8, mitochondrial / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast)
Molecular weightTheoretical: 17.496602 KDa
SequenceString:
MSLVKLANTC AHLQNCSKVR VALTSIPYTK LQLQFAYNLY QQGFLSSLQK GSTMGPDKDF VEVTPDNIST RRLWVGLKYR DNKPVLSSC KLISKPNSRI HLPMEDMKKL CSGVTIRNIK PLQPGELILV RAHNNIMDIN EAISKKLDGE VLCRVK

+
Macromolecule #30: 37S ribosomal protein S9, mitochondrial

MacromoleculeName: 37S ribosomal protein S9, mitochondrial / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast)
Molecular weightTheoretical: 31.980258 KDa
SequenceString: MFSRLSLFRR AALAPAPMRM SFRTIYQKTE DELPRRIVPK LATFYSANPN HEDRINRLER LLRKYIKLPS QNNNEAQQTK APWISFDEY ALIGGGTKLK PTQYTQLLYM LNKLHNIDPQ LTNDEITSEL SQYYKKSSML SNNIKIKTLD EFGRSIAVGK R KSSTAKVF ...String:
MFSRLSLFRR AALAPAPMRM SFRTIYQKTE DELPRRIVPK LATFYSANPN HEDRINRLER LLRKYIKLPS QNNNEAQQTK APWISFDEY ALIGGGTKLK PTQYTQLLYM LNKLHNIDPQ LTNDEITSEL SQYYKKSSML SNNIKIKTLD EFGRSIAVGK R KSSTAKVF VVRGTGEILV NGRQLNDYFL KMKDRESIMY PLQVIESVGK YNIFATTSGG GPTGQAESIM HAIAKALVVF NP LLKSRLH KAGVLTRDYR HVERKKPGKK KARKMPTWVK R

+
Macromolecule #32: 37S ribosomal protein S10, mitochondrial

MacromoleculeName: 37S ribosomal protein S10, mitochondrial / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast)
Molecular weightTheoretical: 23.45892 KDa
SequenceString: MLRNTIALRS FIRTQSTRPY PVNVEAVYYA PLKLPIKYGD LVADIQLRSY DNENLDFYSD FILRTGYYLG IPLTGPKPLP TRRERWTVI KSPFVHAKSK ENFERHTHKR LIRAWDTNPE VLQMLIAYIT KHSMAGVGMK CNFFQRSEIS LDLGSDANGL E KSLSNIDE ...String:
MLRNTIALRS FIRTQSTRPY PVNVEAVYYA PLKLPIKYGD LVADIQLRSY DNENLDFYSD FILRTGYYLG IPLTGPKPLP TRRERWTVI KSPFVHAKSK ENFERHTHKR LIRAWDTNPE VLQMLIAYIT KHSMAGVGMK CNFFQRSEIS LDLGSDANGL E KSLSNIDE LYSLRNDDKA QTSAVGQKVL ELLDSPDFKK HLEKK

+
Macromolecule #33: 37S ribosomal protein S18, mitochondrial

MacromoleculeName: 37S ribosomal protein S18, mitochondrial / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast)
Molecular weightTheoretical: 24.60342 KDa
SequenceString: MLLQPVWKGC RWTQFVRPIR RWNSTGTNRG VPFSFKDISN QEDITNISYP SSSDSVLTKS NGSSEVYKPK EEVVKYILHG KFTKNNTHL TFSSVVEDKN FHKNKGLTYN DTMLYYLNLP QKVKISLSTG CLGFRKAARG EYEAAFQTSG RMFELIKEKN M LNKDIEVV ...String:
MLLQPVWKGC RWTQFVRPIR RWNSTGTNRG VPFSFKDISN QEDITNISYP SSSDSVLTKS NGSSEVYKPK EEVVKYILHG KFTKNNTHL TFSSVVEDKN FHKNKGLTYN DTMLYYLNLP QKVKISLSTG CLGFRKAARG EYEAAFQTSG RMFELIKEKN M LNKDIEVV MDDFGKGRAA FISALVGKEG ASVVKKVVKI SDATKLKFGG VRSPKMRRL

+
Macromolecule #34: unknown protein sequence

MacromoleculeName: unknown protein sequence / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast)
Molecular weightTheoretical: 8.017875 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

+
Macromolecule #35: MRPS12 isoform 1

MacromoleculeName: MRPS12 isoform 1 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: baker's yeast (brewer's yeast)
Molecular weightTheoretical: 16.94874 KDa
SequenceString:
MLSRFMSNTW CTPLRQAQRL FSSTTTMQAT LNQIKRGSGP PRRKKISTAP QLDQCPQRKG VVLRVMVLKP KKPNSAQRKA CRVRLTNGN VVSAYIPGEG HDAQEHSIVY VRGGRCQDLP GVKYHVIRGA GDLSGVVNRI SSRSKYGAKK PSKS

+
Macromolecule #31: 15S ribosomal RNA

MacromoleculeName: 15S ribosomal RNA / type: rna / ID: 31 / Number of copies: 1
Source (natural)Organism: baker's yeast (brewer's yeast)
Molecular weightTheoretical: 549.234062 KDa
SequenceString: UUUUAUAUAA UAAUAAUAAU AUAUAUAUAU AUAUAUUAUU AUAUUAGUUA UAUAAUAAGG AAAAGUAAAA AAUUUAUAAG AAUAUGAUG UUGGUUCAGA UUAAGCGCUA AAUAAGGACA UGACACAUGC GAAUCAUACG UUUAUUAUUG AUAAGAUAAU A AAUAUGUG ...String:
UUUUAUAUAA UAAUAAUAAU AUAUAUAUAU AUAUAUUAUU AUAUUAGUUA UAUAAUAAGG AAAAGUAAAA AAUUUAUAAG AAUAUGAUG UUGGUUCAGA UUAAGCGCUA AAUAAGGACA UGACACAUGC GAAUCAUACG UUUAUUAUUG AUAAGAUAAU A AAUAUGUG GUGUAAACGU GAGUAAUUUU AUUAGGAAUU AAUGAACUAU AGAAUAAGCU AAAUACUUAA UAUAUUAUUA UA UAAAAAU AAUUUAUAUA AUAAAAAGGA UAUAUAUAUA AUAUAUAUUU AUCUAUAGUC AAGCCAAUAA UGGUUUAGGU AGU AGGUUU AUUAAGAGUU AAACCUAGCC AACGAUCCAU AAUCGAUAAU GAAAGUUAGA ACGAUCACGU UGACUCUGAA AUAU AGUCA AUAUCUAUAA GAUACAGCAG UGAGGAAUAU UGGACAAUGA UCGAAAGAUU GAUCCAGUUA CUUAUUAGGA UGAUA UAUA AAAAUAUUUU AUUUUAUUUA UAAAUAUUAA AUAUUUAUAA UAAUAAUAAU AAUAAUAUAU AUAUAUAAAU UGAUUA AAA AUAAAAUCCA UAAAUAAUUA AAAUAAUGAU AUUAAUUACC AUAUAUAUUU UUAUAUGGAU AUAUAUAUUA AUAAUAA UA UUAAUUUUAU UAUUAUUAAU AAUAUAUUUU AAUAGUCCUG ACUAAUAUUU GUGCCAGCAG UCGCGGUAAC ACAAAGAG G GCGAGCGUUA AUCAUAAUGG UUUAAAGGAU CCGUAGAAUG AAUUAUAUAU UAUAAUUUAG AGUUAAUAAA AUAUAAUUA AAGAAUUAUA AUAGUAAAGA UGAAAUAAUA AUAAUAAUUA UAAGACUAAU AUAUGUGAAA AUAUUAAUUA AAUAUUAACU GACAUUGAG GGAUUAAAAC UAGAGUAGCG AAACGGAUUC GAUACCCGUG UAGUUCUAGU AGUAAACUAU GAAUACAAUU A UUUAUAAU AUAUAUUAUA UAUAAAUAAU AAAUGAAAAU GAAAGUAUUC CACCUGAAGA GUACGUUAGC AAUAAUGAAA CU CAAAACA AUAGACGGUU ACAGACUUAA GCAGUGGAGC AUGUUAUUUA AUUCGAUAAU CCACGACUAA CCUUACCAUA UUU UGAAUA UUAUAAUAAU UAUUAUAAUU AUUAUAUUAC AGGCGUUACA UUGUUGUCUU UAGUUCGUGC UGCAAAGUUU UAGA UUAAG UUCAUAAACG AACAAAACUC CAUAUAUAUA AUUUUAAUUA UAUAUAAUUU UAUAUUAUUU AUUAAUAUAA AGAAA GGAA UUAAGACAAA UCAUAAUGAU CCUUAUAAUA UGGGUAAUAG ACGUGCUAUA AUAAAAUGAU AAUAAAAUUA UAUAAA AUA UAUUUAAUUA UAUUUAAUUA AUAAUAUAAA ACAUUUUAAU UUUUAAUAUA UUUUUUUAUU AUAUAUUAAU AUGAAUU AU AAUCUGAAAU UCGAUUAUAU GAAAAAAGAA UUGCUAGUAA UACGUAAAUU AGUAUGUUAC GGUGAAUAUU CUAACUGU U UCGCACUAAU CACUCAUCAC GCGUUGAAAC AUAUUAUUAU CUUAUUAUUU AUAUAAUAUU UUUUAAUAAA UAUUAAUAA UUAUUAAUUU AUAUUUAUUU AUAUCAGAAA UAAUAUGAAU UAAUGCGAAG UUGAAAUACA GUUACCGUAG GGGAACCUGC GGUGGGCUU AUAAAUAUCU UAAAUAUUCU UACA

+
Macromolecule #36: IRON/SULFUR CLUSTER

MacromoleculeName: IRON/SULFUR CLUSTER / type: ligand / ID: 36 / Number of copies: 1 / Formula: SF4
Molecular weightTheoretical: 351.64 Da
Chemical component information

ChemComp-FS1:
IRON/SULFUR CLUSTER / Iron–sulfur cluster

+
Macromolecule #37: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 37 / Number of copies: 72 / Formula: MG
Molecular weightTheoretical: 24.305 Da

+
Macromolecule #38: ADENOSINE-5'-TRIPHOSPHATE

MacromoleculeName: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 38 / Number of copies: 1 / Formula: ATP
Molecular weightTheoretical: 507.181 Da
Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM / Adenosine triphosphate

+
Macromolecule #39: water

MacromoleculeName: water / type: ligand / ID: 39 / Number of copies: 3 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER / Water

-
Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

-
Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.0 µm / Nominal magnification: 64000
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 61.73 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

-
Image processing

Particle selectionNumber selected: 3286640
Startup modelType of model: OTHER / Details: Ab Initio (Cryosparc)
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.1.1)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.1.1)
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.13 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: PHENIX (ver. 1.19) / Number images used: 54519
FSC plot (resolution estimation)

-
Atomic model buiding 1

Initial modelPDB ID:

5mrc

RefinementSpace: REAL
Output model

PDB-8d8k:
Yeast mitochondrial small subunit assembly intermediate (State 2)

+
About Yorodumi

-
News

-
Feb 9, 2022. New format data for meta-information of EMDB entries

New format data for meta-information of EMDB entries

  • Version 3 of the EMDB header file is now the official format.
  • The previous official version 1.9 will be removed from the archive.

Related info.:EMDB header

External links:wwPDB to switch to version 3 of the EMDB data model

-
Aug 12, 2020. Covid-19 info

Covid-19 info

URL: https://pdbj.org/emnavi/covid19.php

New page: Covid-19 featured information page in EM Navigator.

Related info.:Covid-19 info / Mar 5, 2020. Novel coronavirus structure data

+
Mar 5, 2020. Novel coronavirus structure data

Novel coronavirus structure data

Related info.:Yorodumi Speices / Aug 12, 2020. Covid-19 info

External links:COVID-19 featured content - PDBj / Molecule of the Month (242)：Coronavirus Proteases

+
Jan 31, 2019. EMDB accession codes are about to change! (news from PDBe EMDB page)

EMDB accession codes are about to change! (news from PDBe EMDB page)

  • The allocation of 4 digits for EMDB accession codes will soon come to an end. Whilst these codes will remain in use, new EMDB accession codes will include an additional digit and will expand incrementally as the available range of codes is exhausted. The current 4-digit format prefixed with “EMD-” (i.e. EMD-XXXX) will advance to a 5-digit format (i.e. EMD-XXXXX), and so on. It is currently estimated that the 4-digit codes will be depleted around Spring 2019, at which point the 5-digit format will come into force.
  • The EM Navigator/Yorodumi systems omit the EMD- prefix.

Related info.:Q: What is EMD? / ID/Accession-code notation in Yorodumi/EM Navigator

External links:EMDB Accession Codes are Changing Soon! / Contact to PDBj

+
Jul 12, 2017. Major update of PDB

Major update of PDB

  • wwPDB released updated PDB data conforming to the new PDBx/mmCIF dictionary.
  • This is a major update changing the version number from 4 to 5, and with Remediation, in which all the entries are updated.
  • In this update, many items about electron microscopy experimental information are reorganized (e.g. em_software).
  • Now, EM Navigator and Yorodumi are based on the updated data.

External links:wwPDB Remediation / Enriched Model Files Conforming to OneDep Data Standards Now Available in the PDB FTP Archive

-
Yorodumi

Thousand views of thousand structures

  • Yorodumi is a browser for structure data from EMDB, PDB, SASBDB, etc.
  • This page is also the successor to EM Navigator detail page, and also detail information page/front-end page for Omokage search.
  • The word "yorodu" (or yorozu) is an old Japanese word meaning "ten thousand". "mi" (miru) is to see.

Related info.:EMDB / PDB / SASBDB / Comparison of 3 databanks / Yorodumi Search / Aug 31, 2016. New EM Navigator & Yorodumi / Yorodumi Papers / Jmol/JSmol / Function and homology information / Changes in new EM Navigator and Yorodumi

Read more