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- EMDB-26968: Human mitochondrial small subunit assembly intermediate (State C) -

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Basic information

Entry
Database: EMDB / ID: EMD-26968
TitleHuman mitochondrial small subunit assembly intermediate (State C)
Map dataMitochondrial small subunit assembly intermediate (State C)
Sample
  • Complex: Human mitochondrial small subunit assembly intermediate, State C
    • Protein or peptide: x 33 types
    • RNA: x 1 types
  • Ligand: x 10 types
Function / homology
Function and homology information


mitochondrial small ribosomal subunit assembly / mitochondrial transcription factor activity / rRNA modification in the mitochondrion / transcription initiation at mitochondrial promoter / rRNA modification / rRNA (adenine-N6,N6-)-dimethyltransferase activity / Mitochondrial Fatty Acid Beta-Oxidation / mitochondrial ribosome binding / [acyl-carrier-protein] S-malonyltransferase / [acyl-carrier-protein] S-malonyltransferase activity ...mitochondrial small ribosomal subunit assembly / mitochondrial transcription factor activity / rRNA modification in the mitochondrion / transcription initiation at mitochondrial promoter / rRNA modification / rRNA (adenine-N6,N6-)-dimethyltransferase activity / Mitochondrial Fatty Acid Beta-Oxidation / mitochondrial ribosome binding / [acyl-carrier-protein] S-malonyltransferase / [acyl-carrier-protein] S-malonyltransferase activity / mitochondrial ribosome assembly / Mitochondrial translation elongation / Mitochondrial translation termination / Mitochondrial translation initiation / positive regulation of mitochondrial translation / S-adenosyl-L-methionine binding / negative regulation of mitotic nuclear division / mitochondrial ribosome / mitochondrial small ribosomal subunit / rRNA methylation / mitochondrial translation / fatty acid beta-oxidation / mitochondrial nucleoid / positive regulation of proteolysis / ribosomal small subunit binding / Mitochondrial protein degradation / Transferases; Transferring one-carbon groups; Methyltransferases / apoptotic signaling pathway / Transcriptional activation of mitochondrial biogenesis / fatty acid biosynthetic process / rRNA processing / cell junction / regulation of translation / ribosomal small subunit biogenesis / ribosomal small subunit assembly / 4 iron, 4 sulfur cluster binding / small ribosomal subunit / small ribosomal subunit rRNA binding / nuclear membrane / cell population proliferation / tRNA binding / mitochondrial inner membrane / rRNA binding / ribosome / structural constituent of ribosome / mitochondrial matrix / translation / protein domain specific binding / intracellular membrane-bounded organelle / mRNA binding / GTP binding / nucleolus / mitochondrion / DNA binding / RNA binding / nucleoplasm / nucleus / metal ion binding / plasma membrane / cytosol / cytoplasm
Similarity search - Function
: / Putative ribosome-binding factor A, mitochondrial / Ribosomal protein Rsm22-like / : / Mitochondrial small ribosomal subunit Rsm22 / Ribosome-binding factor A, conserved site / Ribosome-binding factor A signature. / Ribosome-binding factor A / Ribosome-binding factor A domain superfamily / Ribosome-binding factor A ...: / Putative ribosome-binding factor A, mitochondrial / Ribosomal protein Rsm22-like / : / Mitochondrial small ribosomal subunit Rsm22 / Ribosome-binding factor A, conserved site / Ribosome-binding factor A signature. / Ribosome-binding factor A / Ribosome-binding factor A domain superfamily / Ribosome-binding factor A / rRNA adenine dimethylase-like, C-terminal / Ribosomal RNA adenine dimethylase / Ribosomal RNA adenine methylase transferase, conserved site / Ribosomal RNA adenine methylase transferase, N-terminal / Ribosomal RNA adenine dimethylases signature. / Ribosomal RNA adenine dimethylases / 28S ribosomal protein S26 / Mitochondrial ribosome subunit S26 / 28S ribosomal protein S27, mitochondrial / MRPS27/PTCD2 / Mitochondrial 28S ribosomal protein S27 / Ribosomal RNA adenine methyltransferase KsgA/Erm / Ribosomal RNA adenine dimethylase / rRNA adenine N(6)-methyltransferase family profile. / Ribosomal protein S22, mitochondrial / Ribosomal protein S23, mitochondrial / Ribosomal protein S23/S25, mitochondrial / Mitochondrial 28S ribosomal protein S31 / : / Mitochondrial 28S ribosomal protein S22 / Mitochondrial ribosomal protein S23 / Mitochondrial 28S ribosomal protein S31 / Ribosomal protein S29, mitochondrial / Ribosomal protein S28, mitochondrial / Mitochondrial 28S ribosomal protein S34 / 28S ribosomal protein S10, mitochondrial / Mitochondrial ribosomal protein MRP-S35 / Mitochondrial 28S ribosomal protein S34 / 28S ribosomal protein S24, mitochondrial / Pentatricopeptide repeat domain-containing protein 3 / 28S ribosomal protein S17, mitochondrial / 28S ribosomal protein S18b, mitochondrial / : / : / Mitochondrial ribosome subunit S24 / Small ribosomal subunit protein uS5m, N-terminal / Small ribosomal subunit protein mS39, PPR / 28S ribosomal protein S25, mitochondrial / Pentatricopeptide repeat domain / Ribosomal protein S27/S33, mitochondrial / Ribosomal protein S24/S35, mitochondrial / Mitochondrial ribosomal subunit S27 / Ribosomal protein S24/S35, mitochondrial, conserved domain / Mitochondrial ribosomal subunit protein / Pentatricopeptide (PPR) repeat profile. / Ribosomal protein S23/S29, mitochondrial / Mitochondrial ribosomal death-associated protein 3 / Pentatricopeptide repeat / Mitochondrial mRNA-processing protein COX24, C-terminal / Mitochondrial mRNA-processing protein COX24, C-terminal / Mitochondrial domain of unknown function (DUF1713) / Malonyl-CoA ACP transacylase, ACP-binding / Acyl transferase / Acyl transferase domain / Acyl transferase domain in polyketide synthase (PKS) enzymes. / Acyl transferase domain superfamily / Acyl transferase/acyl hydrolase/lysophospholipase / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / Ribosomal protein/NADH dehydrogenase domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / Ribosomal protein S21 superfamily / Ribosomal protein S21 / Ribosomal protein S21 / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal protein S18, conserved site / Ribosomal protein S18 signature. / Ribosomal protein S16 / Ribosomal protein S16 domain superfamily / Ribosomal protein S16 / Ribosomal protein S15, bacterial-type / Ribosomal protein S6 / Ribosomal protein S6 / Ribosomal protein S6 superfamily / Ribosomal protein S12, bacterial-type / Translation elongation factor EF1B/ribosomal protein S6 / Ribosomal protein S18 / Ribosomal protein S18 / Ribosomal protein S18 superfamily / Ribosomal protein S2 signature 1. / Ribosomal protein S5, N-terminal, conserved site / Ribosomal protein S5 signature. / Ribosomal protein S2, conserved site / Ribosomal protein S2 / Ribosomal protein S2, flavodoxin-like domain superfamily / Ribosomal protein S2 / Ribosomal protein S5 / S5 double stranded RNA-binding domain profile. / Ribosomal protein S5, N-terminal / Ribosomal protein S5, C-terminal / Ribosomal protein S5, N-terminal domain
Similarity search - Domain/homology
Small ribosomal subunit protein uS12m / Small ribosomal subunit protein uS14m / Small ribosomal subunit protein mS29 / Small ribosomal subunit protein mS22 / Small ribosomal subunit protein mS25 / Small ribosomal subunit protein uS10m / Small ribosomal subunit protein mS35 / Small ribosomal subunit protein uS5m / Small ribosomal subunit protein uS11m / Small ribosomal subunit protein uS15m ...Small ribosomal subunit protein uS12m / Small ribosomal subunit protein uS14m / Small ribosomal subunit protein mS29 / Small ribosomal subunit protein mS22 / Small ribosomal subunit protein mS25 / Small ribosomal subunit protein uS10m / Small ribosomal subunit protein mS35 / Small ribosomal subunit protein uS5m / Small ribosomal subunit protein uS11m / Small ribosomal subunit protein uS15m / Small ribosomal subunit protein bS21m / Small ribosomal subunit protein mS34 / Small ribosomal subunit protein bS6m / Small ribosomal subunit protein uS9m / Malonyl-CoA-acyl carrier protein transacylase, mitochondrial / Putative ribosome-binding factor A, mitochondrial / Dimethyladenosine transferase 1, mitochondrial / Small ribosomal subunit protein mS27 / Small ribosomal subunit protein mS31 / Small ribosomal subunit protein uS3m / Small ribosomal subunit protein mS39 / Small ribosomal subunit protein mS26 / Ribosome assembly protein METTL17, mitochondrial / Small ribosomal subunit protein mS38 / Small ribosomal subunit protein mS33 / Small ribosomal subunit protein bS1m / Small ribosomal subunit protein uS17m / Small ribosomal subunit protein uS7m / Small ribosomal subunit protein uS2m / Small ribosomal subunit protein bS16m / Small ribosomal subunit protein bS18m / Small ribosomal subunit protein mS23 / Small ribosomal subunit protein mS40
Similarity search - Component
Biological speciesHomo sapiens (human) / human (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.54 Å
AuthorsHarper NJ / Burnside C / Klinge S
Funding support United States, 2 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)5T32GM136640-02 United States
The Robertson Foundation
CitationJournal: Nature / Year: 2023
Title: Principles of mitoribosomal small subunit assembly in eukaryotes.
Authors: Nathan J Harper / Chloe Burnside / Sebastian Klinge /
Abstract: Mitochondrial ribosomes (mitoribosomes) synthesize proteins encoded within the mitochondrial genome that are assembled into oxidative phosphorylation complexes. Thus, mitoribosome biogenesis is ...Mitochondrial ribosomes (mitoribosomes) synthesize proteins encoded within the mitochondrial genome that are assembled into oxidative phosphorylation complexes. Thus, mitoribosome biogenesis is essential for ATP production and cellular metabolism. Here we used cryo-electron microscopy to determine nine structures of native yeast and human mitoribosomal small subunit assembly intermediates, illuminating the mechanistic basis for how GTPases are used to control early steps of decoding centre formation, how initial rRNA folding and processing events are mediated, and how mitoribosomal proteins have active roles during assembly. Furthermore, this series of intermediates from two species with divergent mitoribosomal architecture uncovers both conserved principles and species-specific adaptations that govern the maturation of mitoribosomal small subunits in eukaryotes. By revealing the dynamic interplay between assembly factors, mitoribosomal proteins and rRNA that are required to generate functional subunits, our structural analysis provides a vignette for how molecular complexity and diversity can evolve in large ribonucleoprotein assemblies.
History
DepositionMay 13, 2022-
Header (metadata) releaseDec 14, 2022-
Map releaseDec 14, 2022-
UpdateFeb 15, 2023-
Current statusFeb 15, 2023Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_26968.png

Downloads & links

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Map

FileDownload / File: emd_26968.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationMitochondrial small subunit assembly intermediate (State C)
Projections & slices

Image control

Size
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Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.06 Å/pix.
x 400 pix.
= 424.8 Å		1.06 Å/pix.
x 400 pix.
= 424.8 Å		1.06 Å/pix.
x 400 pix.
= 424.8 Å

Surface

Projections

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Images are generated by Spider.

Voxel sizeX=Y=Z: 1.062 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 8.5
Minimum - Maximum-24.33752 - 57.81817
Average (Standard dev.)0.034024987 (±1.3065032)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions400400400
Spacing400400400
CellA=B=C: 424.80002 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_26968_msk_1.map
Projections & Slices
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Histogram

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Half map: Mitochondrial small subunit assembly intermediate (State C)

Fileemd_26968_half_map_1.map
AnnotationMitochondrial small subunit assembly intermediate (State C)
Projections & Slices
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Histogram

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Half map: Mitochondrial small subunit assembly intermediate (State C)

Fileemd_26968_half_map_2.map
AnnotationMitochondrial small subunit assembly intermediate (State C)
Projections & Slices
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Sample components

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Entire : Human mitochondrial small subunit assembly intermediate, State C

EntireName: Human mitochondrial small subunit assembly intermediate, State C
Components
  • Complex: Human mitochondrial small subunit assembly intermediate, State C
    • Protein or peptide: 28S ribosomal protein S34, mitochondrial
    • Protein or peptide: 28S ribosomal protein S35, mitochondrial
    • Protein or peptide: Aurora kinase A-interacting protein
    • Protein or peptide: Pentatricopeptide repeat domain-containing protein 3, mitochondrial
    • Protein or peptide: Dimethyladenosine transferase 1, mitochondrial
    • Protein or peptide: Putative ribosome-binding factor A, mitochondrial
    • Protein or peptide: Methyltransferase-like protein 17, mitochondrial
    • Protein or peptide: Malonyl-CoA-acyl carrier protein transacylase, mitochondrial
    • RNA: 12S mitochondrial rRNA
    • Protein or peptide: 28S ribosomal protein S2, mitochondrial
    • Protein or peptide: 28S ribosomal protein S24, mitochondrial
    • Protein or peptide: 28S ribosomal protein S5, mitochondrial
    • Protein or peptide: 28S ribosomal protein S6, mitochondrial
    • Protein or peptide: 28S ribosomal protein S7, mitochondrial
    • Protein or peptide: 28S ribosomal protein S9, mitochondrial
    • Protein or peptide: 28S ribosomal protein S10, mitochondrial
    • Protein or peptide: 28S ribosomal protein S11, mitochondrial
    • Protein or peptide: 28S ribosomal protein S12, mitochondrial
    • Protein or peptide: 28S ribosomal protein S14, mitochondrial
    • Protein or peptide: 28S ribosomal protein S15, mitochondrial
    • Protein or peptide: 28S ribosomal protein S16, mitochondrial
    • Protein or peptide: 28S ribosomal protein S17, mitochondrial
    • Protein or peptide: 28S ribosomal protein S18b, mitochondrial
    • Protein or peptide: 28S ribosomal protein S18c, mitochondrial
    • Protein or peptide: 28S ribosomal protein S21, mitochondrial
    • Protein or peptide: 28S ribosomal protein S22, mitochondrial
    • Protein or peptide: 28S ribosomal protein S23, mitochondrial
    • Protein or peptide: 28S ribosomal protein S25, mitochondrial
    • Protein or peptide: 28S ribosomal protein S26, mitochondrial
    • Protein or peptide: 28S ribosomal protein S27, mitochondrial
    • Protein or peptide: 28S ribosomal protein S28, mitochondrial
    • Protein or peptide: 28S ribosomal protein S29, mitochondrial
    • Protein or peptide: 28S ribosomal protein S31, mitochondrial
    • Protein or peptide: 28S ribosomal protein S33, mitochondrial
  • Ligand: IRON/SULFUR CLUSTER
  • Ligand: S-ADENOSYLMETHIONINE
  • Ligand: POTASSIUM ION
  • Ligand: MAGNESIUM ION
  • Ligand: NICOTINAMIDE-ADENINE-DINUCLEOTIDE
  • Ligand: ZINC ION
  • Ligand: FE2/S2 (INORGANIC) CLUSTER
  • Ligand: ADENOSINE-5'-TRIPHOSPHATE
  • Ligand: GUANOSINE-5'-DIPHOSPHATE
  • Ligand: water

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Supramolecule #1: Human mitochondrial small subunit assembly intermediate, State C

SupramoleculeName: Human mitochondrial small subunit assembly intermediate, State C
type: complex / ID: 1 / Chimera: Yes / Parent: 0 / Macromolecule list: #1-#34
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 1 MDa

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Macromolecule #1: 28S ribosomal protein S34, mitochondrial

MacromoleculeName: 28S ribosomal protein S34, mitochondrial / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 25.695549 KDa
SequenceString: MARKKVRPRL IAELARRVRA LREQLNRPRD SQLYAVDYET LTRPFSGRRL PVRAWADVRR ESRLLQLLGR LPLFGLGRLV TRKSWLWQH DEPCYWRLTR VRPDYTAQNL DHGKAWGILT FKGKTESEAR EIEHVMYHDW RLVPKHEEEA FTAFTPAPED S LASVPYPP ...String:
MARKKVRPRL IAELARRVRA LREQLNRPRD SQLYAVDYET LTRPFSGRRL PVRAWADVRR ESRLLQLLGR LPLFGLGRLV TRKSWLWQH DEPCYWRLTR VRPDYTAQNL DHGKAWGILT FKGKTESEAR EIEHVMYHDW RLVPKHEEEA FTAFTPAPED S LASVPYPP LLRAMIIAER QKNGDTSTEE PMLNVQRIRM EPWDYPAKQE DKGRAKGTPV

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Macromolecule #2: 28S ribosomal protein S35, mitochondrial

MacromoleculeName: 28S ribosomal protein S35, mitochondrial / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 36.898328 KDa
SequenceString: MAAAALPAWL SLQSRARTLR AFSTAVYSAT PVPTPSLPER TPGNERPPRR KALPPRTEKM AVDQDWPSVY PVAAPFKPSA VPLPVRMGY PVKKGVPMAK EGNLELLKIP NFLHLTPVAI KKHCEALKDF CTEWPAALDS DEKCEKHFPI EIDSTDYVSS G PSVRNPRA ...String:
MAAAALPAWL SLQSRARTLR AFSTAVYSAT PVPTPSLPER TPGNERPPRR KALPPRTEKM AVDQDWPSVY PVAAPFKPSA VPLPVRMGY PVKKGVPMAK EGNLELLKIP NFLHLTPVAI KKHCEALKDF CTEWPAALDS DEKCEKHFPI EIDSTDYVSS G PSVRNPRA RVVVLRVKLS SLNLDDHAKK KLIKLVGERY CKTTDVLTIK TDRCPLRRQN YDYAVYLLTV LYHESWNTEE WE KSKTEAD MEEYIWENSS SERNILETLL QMKAAEKNME INKEELLGTK EIEEYKKSVV SLKNEEENEN SISQYKESVK RLL NVT

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Macromolecule #3: Aurora kinase A-interacting protein

MacromoleculeName: Aurora kinase A-interacting protein / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 22.395326 KDa
SequenceString: MLLGRLTSQL LRAVPWAGGR PPWPVSGVLG SRVCGPLYST SPAGPGRAAS LPRKGAQLEL EEMLVPRKMS VSPLESWLTA RCFLPRLDT GTAGTVAPPQ SYQCPPSQIG EGAEQGDEGV ADAPQIQCKN VLKIRRRKMN HHKYRKLVKK TRFLRRKVQE G RLRRKQIK ...String:
MLLGRLTSQL LRAVPWAGGR PPWPVSGVLG SRVCGPLYST SPAGPGRAAS LPRKGAQLEL EEMLVPRKMS VSPLESWLTA RCFLPRLDT GTAGTVAPPQ SYQCPPSQIG EGAEQGDEGV ADAPQIQCKN VLKIRRRKMN HHKYRKLVKK TRFLRRKVQE G RLRRKQIK FEKDLRRIWL KAGLKEAPEG WQTPKIYLRG K

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Macromolecule #4: Pentatricopeptide repeat domain-containing protein 3, mitochondrial

MacromoleculeName: Pentatricopeptide repeat domain-containing protein 3, mitochondrial
type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 78.648547 KDa
SequenceString: MAVVSAVRWL GLRSRLGQPL TGRRAGLCEQ ARSCRFYSGS ATLSKVEGTD VTGIEEVVIP KKKTWDKVAV LQALASTVNR DTTAVPYVF QDDPYLMPAS SLESRSFLLA KKSGENVAKF IINSYPKYFQ KDIAEPHIPC LMPEYFEPQI KDISEAALKE R IELRKVKA ...String:
MAVVSAVRWL GLRSRLGQPL TGRRAGLCEQ ARSCRFYSGS ATLSKVEGTD VTGIEEVVIP KKKTWDKVAV LQALASTVNR DTTAVPYVF QDDPYLMPAS SLESRSFLLA KKSGENVAKF IINSYPKYFQ KDIAEPHIPC LMPEYFEPQI KDISEAALKE R IELRKVKA SVDMFDQLLQ AGTTVSLETT NSLLDLLCYY GDQEPSTDYH FQQTGQSEAL EEENDETSRR KAGHQFGVTW RA KNNAERI FSLMPEKNEH SYCTMIRGMV KHRAYEQALN LYTELLNNRL HADVYTFNAL IEATVCAINE KFEEKWSKIL ELL RHMVAQ KVKPNLQTFN TILKCLRRFH VFARSPALQV LREMKAIGIE PSLATYHHII RLFDQPGDPL KRSSFIIYDI MNEL MGKRF SPKDPDDDKF FQSAMSICSS LRDLELAYQV HGLLKTGDNW KFIGPDQHRN FYYSKFFDLI CLMEQIDVTL KWYED LIPS AYFPHSQTMI HLLQALDVAN RLEVIPKIWK DSKEYGHTFR SDLREEILML MARDKHPPEL QVAFADCAAD IKSAYE SQP IRQTAQDWPA TSLNCIAILF LRAGRTQEAW KMLGLFRKHN KIPRSELLNE LMDSAKVSNS PSQAIEVVEL ASAFSLP IC EGLTQRVMSD FAINQEQKEA LSNLTALTSD SDTDSSSDSD SDTSEGK

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Macromolecule #5: Dimethyladenosine transferase 1, mitochondrial

MacromoleculeName: Dimethyladenosine transferase 1, mitochondrial / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
EC number: Transferases; Transferring one-carbon groups; Methyltransferases
Source (natural)Organism: human (human)
Molecular weightTheoretical: 39.600953 KDa
SequenceString: MAASGKLSTC RLPPLPTIRE IIKLLRLQAA KQLSQNFLLD LRLTDKIVRK AGNLTNAYVY EVGPGPGGIT RSILNADVAE LLVVEKDTR FIPGLQMLSD AAPGKLRIVH GDVLTFKVEK AFSESLKRPW EDDPPNVHII GNLPFSVSTP LIIKWLENIS C RDGPFVYG ...String:
MAASGKLSTC RLPPLPTIRE IIKLLRLQAA KQLSQNFLLD LRLTDKIVRK AGNLTNAYVY EVGPGPGGIT RSILNADVAE LLVVEKDTR FIPGLQMLSD AAPGKLRIVH GDVLTFKVEK AFSESLKRPW EDDPPNVHII GNLPFSVSTP LIIKWLENIS C RDGPFVYG RTQMTLTFQK EVAERLAANT GSKQRSRLSV MAQYLCNVRH IFTIPGQAFV PKPEVDVGVV HFTPLIQPKI EQ PFKLVEK VVQNVFQFRR KYCHRGLRML FPEAQRLEST GRLLELADID PTLRPRQLSI SHFKSLCDVY RKMCDEDPQL FAY NFREEL KRRKSKNEEK EEDDAENYRL

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Macromolecule #6: Putative ribosome-binding factor A, mitochondrial

MacromoleculeName: Putative ribosome-binding factor A, mitochondrial / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 38.417434 KDa
SequenceString: MWAAAGGLWR SRAGLRALFR SRDAALFPGC ERGLHCSAVS CKNWLKKFAS KTKKKVWYES PSLGSHSTYK PSKLEFLMRS TSKKTRKED HARLRALNGL LYKALTDLLC TPEVSQELYD LNVELSKVSL TPDFSACRAY WKTTLSAEQN AHMEAVLQRS A AHMRHLLM ...String:
MWAAAGGLWR SRAGLRALFR SRDAALFPGC ERGLHCSAVS CKNWLKKFAS KTKKKVWYES PSLGSHSTYK PSKLEFLMRS TSKKTRKED HARLRALNGL LYKALTDLLC TPEVSQELYD LNVELSKVSL TPDFSACRAY WKTTLSAEQN AHMEAVLQRS A AHMRHLLM SQQTLRNVPP IVFVQDKGNA ALAELDQLLA VADFGPRDER DNFVQNDFRD PDAPQPCGTT EPTTSSSLCG ID HEALNKQ IMEYKRRKDK GLGGLVWQGQ VAELTTQMKK GRKRAKPRLE QDSSLKSYLS GEEVEDDLDL VGAPEYECYA PDT EELEAE RGGGRTEDGH SCGASRE

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Macromolecule #7: Methyltransferase-like protein 17, mitochondrial

MacromoleculeName: Methyltransferase-like protein 17, mitochondrial / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
EC number: Transferases; Transferring one-carbon groups; Methyltransferases
Source (natural)Organism: human (human)
Molecular weightTheoretical: 50.807547 KDa
SequenceString: MAAALKCLLT LGRWCPGLGV APQARALAAL VPGVTQVDNK SGFLQKRPHR QHPGILKLPH VRLPQALANG AQLLLLGSAG PTMENQVQT LTSYLWSRHL PVEPEELQRR ARHLEKKFLE NPDLSQTEEK LRGAVLHALR KTTYHWQELS YTEGLSLVYM A ARLDGGFA ...String:
MAAALKCLLT LGRWCPGLGV APQARALAAL VPGVTQVDNK SGFLQKRPHR QHPGILKLPH VRLPQALANG AQLLLLGSAG PTMENQVQT LTSYLWSRHL PVEPEELQRR ARHLEKKFLE NPDLSQTEEK LRGAVLHALR KTTYHWQELS YTEGLSLVYM A ARLDGGFA AVSRAFHEIR ARNPAFQPQT LMDFGSGTGS VTWAAHSIWG QSLREYMCVD RSAAMLVLAE KLLKGGSESG EP YIPGVFF RQFLPVSPKV QFDVVVSAFS LSELPSKADR TEVVQTLWRK TGHFLVLVEN GTKAGHSLLM DARDLVLKGK EKS PLDPRP GFVFAPCPHE LPCPQLTNLA CSFSQAYHPI PFSWNKKPKE EKFSMVILAR GSPEEAHRWP RITQPVLKRP RHVH CHLCC PDGHMQHAVL TARRHGRDLY RCARVSSWGD LLPVLTPSAF PPSTAQDPSE S

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Macromolecule #8: Malonyl-CoA-acyl carrier protein transacylase, mitochondrial

MacromoleculeName: Malonyl-CoA-acyl carrier protein transacylase, mitochondrial
type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO / EC number: [acyl-carrier-protein] S-malonyltransferase
Source (natural)Organism: human (human)
Molecular weightTheoretical: 43.019453 KDa
SequenceString: MSVRVARVAW VRGLGASYRR GASSFPVPPP GAQGVAELLR DATGAEEEAP WAATERRMPG QCSVLLFPGQ GSQVVGMGRG LLNYPRVRE LYAAARRVLG YDLLELSLHG PQETLDRTVH CQPAIFVASL AAVEKLHHLQ PSVIENCVAA AGFSVGEFAA L VFAGAMEF ...String:
MSVRVARVAW VRGLGASYRR GASSFPVPPP GAQGVAELLR DATGAEEEAP WAATERRMPG QCSVLLFPGQ GSQVVGMGRG LLNYPRVRE LYAAARRVLG YDLLELSLHG PQETLDRTVH CQPAIFVASL AAVEKLHHLQ PSVIENCVAA AGFSVGEFAA L VFAGAMEF AEGLYAVKIR AEAMQEASEA VPSGMLSVLG QPQSKFNFAC LEAREHCKSL GIENPVCEVS NYLFPDCRVI SG HQEALRF LQKNSSKFHF RRTRMLPVSG AFHTRLMEPA VEPLTQALKA VDIKKPLVSV YSNVHAHRYR HPGHIHKLLA QQL VSPVKW EQTMHAIYER KKGRGFPQTF EVGPGRQLGA ILKSCNMQAW KSYSAVDVLQ TLEHVDLDPQ EPPR

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Macromolecule #10: 28S ribosomal protein S2, mitochondrial

MacromoleculeName: 28S ribosomal protein S2, mitochondrial / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 33.298238 KDa
SequenceString: MATSSAALPR ILGAGARAPS RWLGFLGKAT PRPARPSRRT LGSATALMIR ESEDSTDFND KILNEPLKHS DFFNVKELFS VRSLFDARV HLGHKAGCRH RFMEPYIFGS RLDHDIIDLE QTATHLQLAL NFTAHMAYRK GIILFISRNR QFSYLIENMA R DCGEYAHT ...String:
MATSSAALPR ILGAGARAPS RWLGFLGKAT PRPARPSRRT LGSATALMIR ESEDSTDFND KILNEPLKHS DFFNVKELFS VRSLFDARV HLGHKAGCRH RFMEPYIFGS RLDHDIIDLE QTATHLQLAL NFTAHMAYRK GIILFISRNR QFSYLIENMA R DCGEYAHT RYFRGGMLTN ARLLFGPTVR LPDLIIFLHT LNNIFEPHVA VRDAAKMNIP TVGIVDTNCN PCLITYPVPG ND DSPLAVH LYCRLFQTAI TRAKEKRQQV EALYRLQGQK EPGDQGPAHP PGADMSHSL

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Macromolecule #11: 28S ribosomal protein S24, mitochondrial

MacromoleculeName: 28S ribosomal protein S24, mitochondrial / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 19.046164 KDa
SequenceString:
MAASVCSGLL GPRVLSWSRE LPCAWRALHT SPVCAKNRAA RVRVSKGDKP VTYEEAHAPH YIAHRKGWLS LHTGNLDGED HAAERTVED VFLRKFMWGT FPGCLADQLV LKRRGNQLEI CAVVLRQLSP HKYYFLVGYS ETLLSYFYKC PVRLHLQTVP S KVVYKYL

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Macromolecule #12: 28S ribosomal protein S5, mitochondrial

MacromoleculeName: 28S ribosomal protein S5, mitochondrial / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 48.094594 KDa
SequenceString: MATAVRAVGC LPVLCSGTAG HLLGRQCSLN TLPAASILAW KSVLGNGHLS SLGTRDTHPY ASLSRALQTQ CCISSPSHLM SQQYRPYSF FTKLTADELW KGALAETGAG AKKGRGKRTK KKKRKDLNRG QIIGEGRYGF LWPGLNVPLM KNGAVQTIAQ R SKEEQEKV ...String:
MATAVRAVGC LPVLCSGTAG HLLGRQCSLN TLPAASILAW KSVLGNGHLS SLGTRDTHPY ASLSRALQTQ CCISSPSHLM SQQYRPYSF FTKLTADELW KGALAETGAG AKKGRGKRTK KKKRKDLNRG QIIGEGRYGF LWPGLNVPLM KNGAVQTIAQ R SKEEQEKV EADMIQQREE WDRKKKMKVK RERGWSGNSW GGISLGPPDP GPCGETYEDF DTRILEVRNV FTMTAKEGRK KS IRVLVAV GNGKGAAGFS IGKATDRMDA FRKAKNRAVH HLHYIERYED HTIFHDISLR FKRTHIKMKK QPKGYGLRCH RAI ITICRL IGIKDMYAKV SGSINMLSLT QGLFRGLSRQ ETHQQLADKK GLHVVEIREE CGPLPIVVAS PRGPLRKDPE PEDE VPDVK LDWEDVKTAQ GMKRSVWSNL KRAAT

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Macromolecule #13: 28S ribosomal protein S6, mitochondrial

MacromoleculeName: 28S ribosomal protein S6, mitochondrial / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 14.250588 KDa
SequenceString:
MPRYELALIL KAMQRPETAA TLKRTIEALM DRGAIVRDLE NLGERALPYR ISAHSQQHNR GGYFLVDFYA PTAAVESMVE HLSRDIDVI RGNIVKHPLT QELKECEGIV PVPLAEKLYS TKKRKK

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Macromolecule #14: 28S ribosomal protein S7, mitochondrial

MacromoleculeName: 28S ribosomal protein S7, mitochondrial / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 28.186154 KDa
SequenceString: MAAPAVKVAR GWSGLALGVR RAVLQLPGLT QVRWSRYSPE FKDPLIDKEY YRKPVEELTE EEKYVRELKK TQLIKAAPAG KTSSVFEDP VISKFTNMMM IGGNKVLARS LMIQTLEAVK RKQFEKYHAA SAEEQATIER NPYTIFHQAL KNCEPMIGLV P ILKGGRFY ...String:
MAAPAVKVAR GWSGLALGVR RAVLQLPGLT QVRWSRYSPE FKDPLIDKEY YRKPVEELTE EEKYVRELKK TQLIKAAPAG KTSSVFEDP VISKFTNMMM IGGNKVLARS LMIQTLEAVK RKQFEKYHAA SAEEQATIER NPYTIFHQAL KNCEPMIGLV P ILKGGRFY QVPVPLPDRR RRFLAMKWMI TECRDKKHQR TLMPEKLSHK LLEAFHNQGP VIKRKHDLHK MAEANRALAH YR WW

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Macromolecule #15: 28S ribosomal protein S9, mitochondrial

MacromoleculeName: 28S ribosomal protein S9, mitochondrial / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 45.909859 KDa
SequenceString: MAAPCVSYGG AVSYRLLLWG RGSLARKQGL WKTAAPELQT NVRSQILRLR HTAFVIPKKN VPTSKRETYT EDFIKKQIEE FNIGKRHLA NMMGEDPETF TQEDIDRAIA YLFPSGLFEK RARPVMKHPE QIFPRQRAIQ WGEDGRPFHY LFYTGKQSYY S LMHDVYGM ...String:
MAAPCVSYGG AVSYRLLLWG RGSLARKQGL WKTAAPELQT NVRSQILRLR HTAFVIPKKN VPTSKRETYT EDFIKKQIEE FNIGKRHLA NMMGEDPETF TQEDIDRAIA YLFPSGLFEK RARPVMKHPE QIFPRQRAIQ WGEDGRPFHY LFYTGKQSYY S LMHDVYGM LLNLEKHQSH LQAKSLLPEK TVTRDVIGSR WLIKEELEEM LVEKLSDLDY MQFIRLLEKL LTSQCGAAEE EF VQRFRRS VTLESKKQLI EPVQYDEQGM AFSKSEGKRK TAKAEAIVYK HGSGRIKVNG IDYQLYFPIT QDREQLMFPF HFV DRLGKH DVTCTVSGGG RSAQAGAIRL AMAKALCSFV TEDEVEWMRQ AGLLTTDPRV RERKKPGQEG ARRKFTWKKR

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Macromolecule #16: 28S ribosomal protein S10, mitochondrial

MacromoleculeName: 28S ribosomal protein S10, mitochondrial / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 23.0335 KDa
SequenceString: MAARTAFGAV CRRLWQGLGN FSVNTSKGNT AKNGGLLLST NMKWVQFSNL HVDVPKDLTK PVVTISDEPD ILYKRLSVLV KGHDKAVLD SYEYFAVLAA KELGISIKVH EPPRKIERFT LLQSVHIYKK HRVQYEMRTL YRCLELEHLT GSTADVYLEY I QRNLPEGV ...String:
MAARTAFGAV CRRLWQGLGN FSVNTSKGNT AKNGGLLLST NMKWVQFSNL HVDVPKDLTK PVVTISDEPD ILYKRLSVLV KGHDKAVLD SYEYFAVLAA KELGISIKVH EPPRKIERFT LLQSVHIYKK HRVQYEMRTL YRCLELEHLT GSTADVYLEY I QRNLPEGV AMEVTKTQLE QLPEHIKEPI WETLSEEKEE SKS

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Macromolecule #17: 28S ribosomal protein S11, mitochondrial

MacromoleculeName: 28S ribosomal protein S11, mitochondrial / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 20.648793 KDa
SequenceString: MQAVRNAGSR FLRSWTWPQT AGRVVARTPA GTICTGARQL QDAAAKQKVE QNAAPSHTKF SIYPPIPGEE SSLRWAGKKF EEIPIAHIK ASHNNTQIQV VSASNEPLAF ASCGTEGFRN AKKGTGIAAQ TAGIAAAARA KQKGVIHIRV VVKGLGPGRL S AMHGLIMG ...String:
MQAVRNAGSR FLRSWTWPQT AGRVVARTPA GTICTGARQL QDAAAKQKVE QNAAPSHTKF SIYPPIPGEE SSLRWAGKKF EEIPIAHIK ASHNNTQIQV VSASNEPLAF ASCGTEGFRN AKKGTGIAAQ TAGIAAAARA KQKGVIHIRV VVKGLGPGRL S AMHGLIMG GLEVISITDN TPIPHNGCRP RKARKL

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Macromolecule #18: 28S ribosomal protein S12, mitochondrial

MacromoleculeName: 28S ribosomal protein S12, mitochondrial / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 15.200947 KDa
SequenceString:
MSWSGLLHGL NTSLTCGPAL VPRLWATCSM ATLNQMHRLG PPKRPPRKLG PTEGRPQLKG VVLCTFTRKP KKPNSANRKC CRVRLSTGR EAVCFIPGEG HTLQEHQIVL VEGGRTQDLP GVKLTVVRGK YDCGHVQKK

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Macromolecule #19: 28S ribosomal protein S14, mitochondrial

MacromoleculeName: 28S ribosomal protein S14, mitochondrial / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 15.168788 KDa
SequenceString:
MAAFMLGSLL RTFKQMVPSS ASGQVRSHYV DWRMWRDVKR RKMAYEYADE RLRINSLRKN TILPKILQDV ADEEIAALPR DSCPVRIRN RCVMTSRPRG VKRRWRLSRI VFRHLADHGQ LSGIQRATW

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Macromolecule #20: 28S ribosomal protein S15, mitochondrial

MacromoleculeName: 28S ribosomal protein S15, mitochondrial / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 29.903293 KDa
SequenceString: MLRVAWRTLS LIRTRAVTQV LVPGLPGGGS AKFPFNQWGL QPRSLLLQAA RGYVVRKPAQ SRLDDDPPPS TLLKDYQNVP GIEKVDDVV KRLLSLEMAN KKEMLKIKQE QFMKKIVANP EDTRSLEARI IALSVKIRSY EEHLEKHRKD KAHKRYLLMS I DQRKKMLK ...String:
MLRVAWRTLS LIRTRAVTQV LVPGLPGGGS AKFPFNQWGL QPRSLLLQAA RGYVVRKPAQ SRLDDDPPPS TLLKDYQNVP GIEKVDDVV KRLLSLEMAN KKEMLKIKQE QFMKKIVANP EDTRSLEARI IALSVKIRSY EEHLEKHRKD KAHKRYLLMS I DQRKKMLK NLRNTNYDVF EKICWGLGIE YTFPPLYYRR AHRRFVTKKA LCIRVFQETQ KLKKRRRALK AAAAAQKQAK RR NPDSPAK AIPKTLKDSQ

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Macromolecule #21: 28S ribosomal protein S16, mitochondrial

MacromoleculeName: 28S ribosomal protein S16, mitochondrial / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 15.371899 KDa
SequenceString:
MVHLTTLLCK AYRGGHLTIR LALGGCTNRP FYRIVAAHNK CPRDGRFVEQ LGSYDPLPNS HGEKLVALNL DRIRHWIGCG AHLSKPMEK LLGLAGFFPL HPMMITNAER LRRKRAREVL LASQKTDAEA TDTEATET

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Macromolecule #22: 28S ribosomal protein S17, mitochondrial

MacromoleculeName: 28S ribosomal protein S17, mitochondrial / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 14.526078 KDa
SequenceString:
MSVVRSSVHA RWIVGKVIGT KMQKTAKVRV TRLVLDPYLL KYFNKRKTYF AHDALQQCTV GDIVLLRALP VPRAKHVKHE LAEIVFKVG KVIDPVTGKP CAGTTYLESP LSSETTQLSK NLEELNISSA Q

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Macromolecule #23: 28S ribosomal protein S18b, mitochondrial

MacromoleculeName: 28S ribosomal protein S18b, mitochondrial / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 29.440629 KDa
SequenceString: MAASVLNTVL RRLPMLSLFR GSHRVQVPLQ TLCTKAPSEE DSLSSVPISP YKDEPWKYLE SEEYQERYGS RPVWADYRRN HKGGVPPQR TRKTCIRRNK VVGNPCPICR DHKLHVDFRN VKLLEQFVCA HTGIIFYAPY TGVCVKQHKR LTQAIQKARD H GLLIYHIP ...String:
MAASVLNTVL RRLPMLSLFR GSHRVQVPLQ TLCTKAPSEE DSLSSVPISP YKDEPWKYLE SEEYQERYGS RPVWADYRRN HKGGVPPQR TRKTCIRRNK VVGNPCPICR DHKLHVDFRN VKLLEQFVCA HTGIIFYAPY TGVCVKQHKR LTQAIQKARD H GLLIYHIP QVEPRDLDFS TSHGAVSATP PAPTLVSGDP WYPWYNWKQP PERELSRLRR LYQGHLQEES GPPPESMPKM PP RTPAEAS STGQTGPQSA L

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Macromolecule #24: 28S ribosomal protein S18c, mitochondrial

MacromoleculeName: 28S ribosomal protein S18c, mitochondrial / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 15.876786 KDa
SequenceString:
MAAVVAVCGG LGRKKLTHLV TAAVSLTHPG THTVLWRRGC SQQVSSNEDL PISMENPYKE PLKKCILCGK HVDYKNVQLL SQFVSPFTG CIYGRHITGL CGKKQKEITK AIKRAQIMGF MPVTYKDPAY LKDPKVCNIR YRE

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Macromolecule #25: 28S ribosomal protein S21, mitochondrial

MacromoleculeName: 28S ribosomal protein S21, mitochondrial / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 10.806689 KDa
SequenceString:
M(AYA)KHLKFIAR TVMVQEGNVE SAYRTLNRIL TMDGLIEDIK HRRYYEKPCR RRQRESYERC RRIYNMEMAR KINFLM RKN RADPWQGC

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Macromolecule #26: 28S ribosomal protein S22, mitochondrial

MacromoleculeName: 28S ribosomal protein S22, mitochondrial / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 41.337316 KDa
SequenceString: MAPLGTTVLL WSLLRSSPGV ERVCFRARIQ PWHGGLLQPL PCSFEMGLPR RRFSSEAAES GSPETKKPTF MDEEVQSILT KMTGLNLQK TFKPAIQELK PPTYKLMTQA QLEEATRQAV EAAKVRLKMP PVLEERVPIN DVLAEDKILE GTETTKYVFT D ISYSIPHR ...String:
MAPLGTTVLL WSLLRSSPGV ERVCFRARIQ PWHGGLLQPL PCSFEMGLPR RRFSSEAAES GSPETKKPTF MDEEVQSILT KMTGLNLQK TFKPAIQELK PPTYKLMTQA QLEEATRQAV EAAKVRLKMP PVLEERVPIN DVLAEDKILE GTETTKYVFT D ISYSIPHR ERFIVVREPS GTLRKASWEE RDRMIQVYFP KEGRKILTPI IFKEENLRTM YSQDRHVDVL NLCFAQFEPD ST EYIKVHH KTYEDIDKRG KYDLLRSTRY FGGMVWYFVN NKKIDGLLID QIQRDLIDDA TNLVQLYHVL HPDGQSAQGA KDQ AAEGIN LIKVFAKTEA QKGAYIELTL QTYQEALSRH SAAS

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Macromolecule #27: 28S ribosomal protein S23, mitochondrial

MacromoleculeName: 28S ribosomal protein S23, mitochondrial / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 21.805664 KDa
SequenceString:
MAGSRLETVG SIFSRTRDLV RAGVLKEKPL WFDVYDAFPP LREPVFQRPR VRYGKAKAPI QDIWYHEDRI RAKFYSVYGS GQRAFDLFN PNFKSTCQRF VEKYTELQKL GETDEEKLFV ETGKALLAEG VILRRVGEAR TQHGGSHVSR KSEHLSVRPQ T ALEENETQ KEVPQDQHLE APADQSKGLL PP

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Macromolecule #28: 28S ribosomal protein S25, mitochondrial

MacromoleculeName: 28S ribosomal protein S25, mitochondrial / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 20.146428 KDa
SequenceString:
MPMKGRFPIR RTLQYLSQGN VVFKDSVKVM TVNYNTHGEL GEGARKFVFF NIPQIQYKNP WVQIMMFKNM TPSPFLRFYL DSGEQVLVD VETKSNKEIM EHIRKILGKN EETLREEEEE KKQLSHPANF GPRKYCLREC ICEVEGQVPC PSLVPLPKEM R GKYKAALK ADAQD

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Macromolecule #29: 28S ribosomal protein S26, mitochondrial

MacromoleculeName: 28S ribosomal protein S26, mitochondrial / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 24.25982 KDa
SequenceString: MLRALSRLGA GTPCRPRAPL VLPARGRKTR HDPLAKSKIE RVNMPPAVDP AEFFVLMERY QHYRQTVRAL RMEFVSEVQR KVHEARAGV LAERKALKDA AEHRELMAWN QAENRRLHEL RIARLRQEER EQEQRQALEQ ARKAEEVQAW AQRKEREVLQ L QEEVKNFI ...String:
MLRALSRLGA GTPCRPRAPL VLPARGRKTR HDPLAKSKIE RVNMPPAVDP AEFFVLMERY QHYRQTVRAL RMEFVSEVQR KVHEARAGV LAERKALKDA AEHRELMAWN QAENRRLHEL RIARLRQEER EQEQRQALEQ ARKAEEVQAW AQRKEREVLQ L QEEVKNFI TRENLEARVE AALDSRKNYN WAITREGLVV RPQRRDS

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Macromolecule #30: 28S ribosomal protein S27, mitochondrial

MacromoleculeName: 28S ribosomal protein S27, mitochondrial / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 47.669262 KDa
SequenceString: MAASIVRRGM LLARQVVLPQ LSPAGKRYLL SSAYVDSHKW EAREKEHYCL ADLASLMDKT FERKLPVSSL TISRLIDNIS SREEIDHAE YYLYKFRHSP NCWYLRNWTI HTWIRQCLKY DAQDKALYTL VNKVQYGIFP DNFTFNLLMD SFIKKENYKD A LSVVFEVM ...String:
MAASIVRRGM LLARQVVLPQ LSPAGKRYLL SSAYVDSHKW EAREKEHYCL ADLASLMDKT FERKLPVSSL TISRLIDNIS SREEIDHAE YYLYKFRHSP NCWYLRNWTI HTWIRQCLKY DAQDKALYTL VNKVQYGIFP DNFTFNLLMD SFIKKENYKD A LSVVFEVM MQEAFEVPST QLLSLYVLFH CLAKKTDFSW EEERNFGASL LLPGLKQKNS VGFSSQLYGY ALLGKVELQQ GL RAVYHNM PLIWKPGYLD RALQVMEKVA ASPEDIKLCR EALDVLGAVL KALTSADGAS EEQSQNDEDN QGSEKLVEQL DIE ETEQSK LPQYLERFKA LHSKLQALGK IESEGLLSLT TQLVKEKLST CEAEDIATYE QNLQQWHLDL VQLIQREQQQ REQA KQEYQ AQKAAKASA

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Macromolecule #31: 28S ribosomal protein S28, mitochondrial

MacromoleculeName: 28S ribosomal protein S28, mitochondrial / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 20.878846 KDa
SequenceString:
MAALCRTRAV AAESHFLRVF LFFRPFRGVG TESGSESGSS NAKEPKTRAG GFASALERHS ELLQKVEPLQ KGSPKNVESF ASMLRHSPL TQMGPAKDKL VIGRIFHIVE NDLYIDFGGK FHCVCRRPEV DGEKYQKGTR VRLRLLDLEL TSRFLGATTD T TVLEANAV LLGIQESKDS RSKEEHHEK

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Macromolecule #32: 28S ribosomal protein S29, mitochondrial

MacromoleculeName: 28S ribosomal protein S29, mitochondrial / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 45.634406 KDa
SequenceString: MMLKGITRLI SRIHKLDPGR FLHMGTQARQ SIAAHLDNQV PVESPRAISR TNENDPAKHG DQHEGQHYNI SPQDLETVFP HGLPPRFVM QVKTFSEACL MVRKPALELL HYLKNTSFAY PAIRYLLYGE KGTGKTLSLC HVIHFCAKQD WLILHIPDAH L WVKNCRDL ...String:
MMLKGITRLI SRIHKLDPGR FLHMGTQARQ SIAAHLDNQV PVESPRAISR TNENDPAKHG DQHEGQHYNI SPQDLETVFP HGLPPRFVM QVKTFSEACL MVRKPALELL HYLKNTSFAY PAIRYLLYGE KGTGKTLSLC HVIHFCAKQD WLILHIPDAH L WVKNCRDL LQSSYNKQRF DQPLEASTWL KNFKTTNERF LNQIKVQEKY VWNKRESTEK GSPLGEVVEQ GITRVRNATD AV GIVLKEL KRQSSLGMFH LLVAVDGINA LWGRTTLKRE DKSPIAPEEL ALVHNLRKMM KNDWHGGAIV SALSQTGSLF KPR KAYLPQ ELLGKEGFDA LDPFIPILVS NYNPKEFESC IQYYLENNWL QHEKAPTEEG KKELLFLSNA NPSLLERHCA YL

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Macromolecule #33: 28S ribosomal protein S31, mitochondrial

MacromoleculeName: 28S ribosomal protein S31, mitochondrial / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 45.391473 KDa
SequenceString: MFPRVSTFLP LRPLSRHPLS SGSPETSAAA IMLLTVRHGT VRYRSSALLA RTKNNIQRYF GTNSVICSKK DKQSVRTEET SKETSESQD SEKENTKKDL LGIIKGMKVE LSTVNVRTTK PPKRRPLKSL EATLGRLRRA TEYAPKKRIE PLSPELVAAA S AVADSLPF ...String:
MFPRVSTFLP LRPLSRHPLS SGSPETSAAA IMLLTVRHGT VRYRSSALLA RTKNNIQRYF GTNSVICSKK DKQSVRTEET SKETSESQD SEKENTKKDL LGIIKGMKVE LSTVNVRTTK PPKRRPLKSL EATLGRLRRA TEYAPKKRIE PLSPELVAAA S AVADSLPF DKQTTKSELL SQLQQHEEES RAQRDAKRPK ISFSNIISDM KVARSATARV RSRPELRIQF DEGYDNYPGQ EK TDDLKKR KNIFTGKRLN IFDMMAVTKE APETDTSPSL WDVEFAKQLA TVNEQPLQNG FEELIQWTKE GKLWEFPINN EAG FDDDGS EFHEHIFLEK HLESFPKQGP IRHFMELVTC GLSKNPYLSV KQKVEHIEWF RNYFNEKKDI LKESNIQFN

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Macromolecule #34: 28S ribosomal protein S33, mitochondrial

MacromoleculeName: 28S ribosomal protein S33, mitochondrial / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 12.657776 KDa
SequenceString:
MSSLSEYAFR MSRLSARLFG EVTRPTNSKS MKVVKLFSEL PLAKKKETYD WYPNHHTYAE LMQTLRFLGL YRDEHQDFMD EQKRLKKLR GKEKPKKGEG KRAAKRK

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Macromolecule #9: 12S mitochondrial rRNA

MacromoleculeName: 12S mitochondrial rRNA / type: rna / ID: 9 / Number of copies: 1
Source (natural)Organism: human (human)
Molecular weightTheoretical: 306.449312 KDa
SequenceString: AAUAGGUUUG GUCCUAGCCU UUCUAUUAGC UCUUAGUAAG AUUACACAUG CAAGCAUCCC CGUUCCAGUG AGUUCACCCU CUAAAUCAC CACGAUCAAA AGGGACAAGC AUCAAGCACG CAGCAAUGCA GCUCAAAACG CUUAGCCUAG CCACACCCCC A CGGGAAAC ...String:
AAUAGGUUUG GUCCUAGCCU UUCUAUUAGC UCUUAGUAAG AUUACACAUG CAAGCAUCCC CGUUCCAGUG AGUUCACCCU CUAAAUCAC CACGAUCAAA AGGGACAAGC AUCAAGCACG CAGCAAUGCA GCUCAAAACG CUUAGCCUAG CCACACCCCC A CGGGAAAC AGCAGUGAUU AACCUUUAGC AAUAAACGAA AGUUUAACUA AGCUAUACUA ACCCCAGGGU UGGUCAAUUU CG UGCCAGC CACCGCGGUC ACACGAUUAA CCCAAGUCAA UAGAAGCCGG CGUAAAGAGU GUUUUAGAUC ACCCCCUCCC CAA UAAAGC UAAAACUCAC CUGAGUUGUA AAAAACUCCA GUUGACACAA AAUAGACUAC GAAAGUGGCU UUAACAUAUC UGAA CACAC AAUAGCUAAG ACCCAAACUG GGAUUAGAUA CCCCACUAUG CUUAGCCCUA AACCUCAACA GUUAAAUCAA CAAAA CUGC UCGCCAGAAC ACUACGAGCC ACAGCUUAAA ACUCAAAGGA CCUGGCGGUG CUUCAUAUCC CUCUAGAGGA GCCUGU UCU GUAAUCGAUA AACCCCGAUC AACCUCACCA CCUCUUGCUC AGCCUAUAUA CCGCCAUCUU CAGCAAACCC UGAUGAA GG CUACAAAGUA AGCGCAAGUA CCCACGUAAA GACGUUAGGU CAAGGUGUAG CCCAUGAGGU GGCAAGAAAU GGGCUACA U UUUCUACCCC AGAAAACUAC GAUAGCCCUU AUGAAACUUA AGGGUCGAAG GUGGAUUUAG CAGUAAACUG AGAGUAGAG UGCUUAGUUG AACAGGGCCC UGAAGCGCGU ACACACCGCC CGUCACCCUC CUCAAGUAUA CUUCAAAGGA CAUUUAACUA AAACCCCUA CGCAUUUAUA UAGAGGAGAC AAGUCGUAAC AUGGUAAGUG UACUGGAAAG UGCACUUGGA CGAACC

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Macromolecule #35: IRON/SULFUR CLUSTER

MacromoleculeName: IRON/SULFUR CLUSTER / type: ligand / ID: 35 / Number of copies: 1 / Formula: SF4
Molecular weightTheoretical: 351.64 Da
Chemical component information

ChemComp-FS1:
IRON/SULFUR CLUSTER

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Macromolecule #36: S-ADENOSYLMETHIONINE

MacromoleculeName: S-ADENOSYLMETHIONINE / type: ligand / ID: 36 / Number of copies: 1 / Formula: SAM
Molecular weightTheoretical: 398.437 Da
Chemical component information

ChemComp-SAM:
S-ADENOSYLMETHIONINE

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Macromolecule #37: POTASSIUM ION

MacromoleculeName: POTASSIUM ION / type: ligand / ID: 37 / Number of copies: 5 / Formula: K
Molecular weightTheoretical: 39.098 Da

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Macromolecule #38: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 38 / Number of copies: 31 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #39: NICOTINAMIDE-ADENINE-DINUCLEOTIDE

MacromoleculeName: NICOTINAMIDE-ADENINE-DINUCLEOTIDE / type: ligand / ID: 39 / Number of copies: 1 / Formula: NAD
Molecular weightTheoretical: 663.425 Da
Chemical component information

ChemComp-NAD:
NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NAD*YM

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Macromolecule #40: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 40 / Number of copies: 1 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #41: FE2/S2 (INORGANIC) CLUSTER

MacromoleculeName: FE2/S2 (INORGANIC) CLUSTER / type: ligand / ID: 41 / Number of copies: 2 / Formula: FES
Molecular weightTheoretical: 175.82 Da
Chemical component information

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER

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Macromolecule #42: ADENOSINE-5'-TRIPHOSPHATE

MacromoleculeName: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 42 / Number of copies: 1 / Formula: ATP
Molecular weightTheoretical: 507.181 Da
Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

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Macromolecule #43: GUANOSINE-5'-DIPHOSPHATE

MacromoleculeName: GUANOSINE-5'-DIPHOSPHATE / type: ligand / ID: 43 / Number of copies: 1 / Formula: GDP
Molecular weightTheoretical: 443.201 Da
Chemical component information

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

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Macromolecule #44: water

MacromoleculeName: water / type: ligand / ID: 44 / Number of copies: 46 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Number grids imaged: 1 / Number real images: 47037 / Average electron dose: 51.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.8 µm / Nominal defocus min: 0.7000000000000001 µm / Nominal magnification: 64000
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 9109335
Startup modelType of model: OTHER / Details: Ab initio (RELION 3.1.1)
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.54 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: PHENIX (ver. 1.19)
Details: 12 focused maps calculated in RELION 3.1.1 were combined into a composite map using phenix.combine_focused_maps. Composite half maps were generated by combining each half map from focused refinements.
Number images used: 262363
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.1.1)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.1.1)
FSC plot (resolution estimation)

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Atomic model buiding 1

Initial model
PDB IDChain

6rw4


2c2n

chain_id: A

6aax

chain_id: A
RefinementSpace: REAL
Output model

PDB-8csr:
Human mitochondrial small subunit assembly intermediate (State C)

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