National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
R01-GM084192
米国
引用
ジャーナル: Nat Struct Mol Biol / 年: 2022 タイトル: Nucleosome recognition and DNA distortion by the Chd1 remodeler in a nucleotide-free state. 著者: Ilana M Nodelman / Sayan Das / Anneliese M Faustino / Stephen D Fried / Gregory D Bowman / Jean-Paul Armache / 要旨: Chromatin remodelers are ATP-dependent enzymes that reorganize nucleosomes within all eukaryotic genomes. Here we report a complex of the Chd1 remodeler bound to a nucleosome in a nucleotide-free ...Chromatin remodelers are ATP-dependent enzymes that reorganize nucleosomes within all eukaryotic genomes. Here we report a complex of the Chd1 remodeler bound to a nucleosome in a nucleotide-free state, determined by cryo-EM to 2.3 Å resolution. The remodeler stimulates the nucleosome to absorb an additional nucleotide on each strand at two different locations: on the tracking strand within the ATPase binding site and on the guide strand one helical turn from the ATPase motor. Remarkably, the additional nucleotide on the tracking strand is associated with a local transformation toward an A-form geometry, explaining how sequential ratcheting of each DNA strand occurs. The structure also reveals a histone-binding motif, ChEx, which can block opposing remodelers on the nucleosome and may allow Chd1 to participate in histone reorganization during transcription.
EMPIAR-10876 (タイトル: 2.3 A structure of the ATP-dependent chromatin remodeler Chd1 bound to the nucleosome in a nucleotide-free state Data size: 4.7 TB Data #1: Unaligned frames of Chd1-bound nucleosomes collected on Gatan K3 in Super Resolution [micrographs - multiframe] Data #2: MotionCor2-aligned frames of Chd1-bound nucleosomes collected on Gatan K3 [micrographs - single frame] Data #3: Processed subsets [picked particles - single frame - processed])
全体 : Chd1 ATP-dependent chromatin remodeler bound to a nucleosome in a...
全体
名称: Chd1 ATP-dependent chromatin remodeler bound to a nucleosome in a nucleotide-free state
要素
複合体: Chd1 ATP-dependent chromatin remodeler bound to a nucleosome in a nucleotide-free state
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超分子 #1: Chd1 ATP-dependent chromatin remodeler bound to a nucleosome in a...
超分子
名称: Chd1 ATP-dependent chromatin remodeler bound to a nucleosome in a nucleotide-free state タイプ: complex / ID: 1 / 親要素: 0 / 詳細: This entry has a weak DNA-binding domain
由来(天然)
生物種: Saccharomyces cerevisiae (パン酵母)
分子量
理論値: 400 KDa
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実験情報
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構造解析
手法
クライオ電子顕微鏡法
解析
単粒子再構成法
試料の集合状態
particle
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試料調製
濃度
1.7 mg/mL
緩衝液
pH: 7 詳細: 20 mM HEPES, pH 7.0, 60 mM KCl, 1.5 mM DTT, 1 mM MgCl2