National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
R01 GM30598
United States
Citation
Journal: Life Sci Alliance / Year: 2020 Title: CryoEM structure of flight muscle thick filaments at 7 Å resolution. Authors: Nadia Daneshparvar / Dianne W Taylor / Thomas S O'Leary / Hamidreza Rahmani / Fatemeh Abbasiyeganeh / Michael J Previs / Kenneth A Taylor / Abstract: Striated muscle thick filaments are composed of myosin II and several non-myosin proteins. Myosin II's long α-helical coiled-coil tail forms the dense protein backbone of filaments, whereas its N- ...Striated muscle thick filaments are composed of myosin II and several non-myosin proteins. Myosin II's long α-helical coiled-coil tail forms the dense protein backbone of filaments, whereas its N-terminal globular head containing the catalytic and actin-binding activities extends outward from the backbone. Here, we report the structure of thick filaments of the flight muscle of the fruit fly at 7 Å resolution. Its myosin tails are arranged in curved molecular crystalline layers identical to flight muscles of the giant water bug Four non-myosin densities are observed, three of which correspond to ones found in ; one new density, possibly stretchin-mlck, is found on the backbone outer surface. Surprisingly, the myosin heads are disordered rather than ordered along the filament backbone. Our results show striking myosin tail similarity within flight muscle filaments of two insect orders separated by several hundred million years of evolution.
History
Deposition
Jun 26, 2020
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Header (metadata) release
Jul 8, 2020
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Map release
Jul 8, 2020
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Update
Jul 8, 2020
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Current status
Jul 8, 2020
Processing site: RCSB / Status: Released
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Structure visualization
Movie
Surface view with section colored by density value
EMPIAR-10436 (Title: The CryoEM Structure of Drosophila Flight Muscle Thick Filaments at 7Å Resolution Data size: 12.0 TB / Data #1: Wild Type Drosophila [micrographs - multiframe])
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