+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-22217 | |||||||||
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Title | Drosophila Flight Muscle Thick Filament | |||||||||
Map data | ||||||||||
Sample |
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Biological species | Drosophila melanogaster (fruit fly) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 7.0 Å | |||||||||
Authors | Daneshparvar N / Taylor DW / O'Leary T / Rahmani H / Abbasi Yeganeh F / Taylor KA / Previs MJ | |||||||||
Funding support | United States, 1 items
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Citation | Journal: Life Sci Alliance / Year: 2020 Title: CryoEM structure of flight muscle thick filaments at 7 Å resolution. Authors: Nadia Daneshparvar / Dianne W Taylor / Thomas S O'Leary / Hamidreza Rahmani / Fatemeh Abbasiyeganeh / Michael J Previs / Kenneth A Taylor / Abstract: Striated muscle thick filaments are composed of myosin II and several non-myosin proteins. Myosin II's long α-helical coiled-coil tail forms the dense protein backbone of filaments, whereas its N- ...Striated muscle thick filaments are composed of myosin II and several non-myosin proteins. Myosin II's long α-helical coiled-coil tail forms the dense protein backbone of filaments, whereas its N-terminal globular head containing the catalytic and actin-binding activities extends outward from the backbone. Here, we report the structure of thick filaments of the flight muscle of the fruit fly at 7 Å resolution. Its myosin tails are arranged in curved molecular crystalline layers identical to flight muscles of the giant water bug Four non-myosin densities are observed, three of which correspond to ones found in ; one new density, possibly stretchin-mlck, is found on the backbone outer surface. Surprisingly, the myosin heads are disordered rather than ordered along the filament backbone. Our results show striking myosin tail similarity within flight muscle filaments of two insect orders separated by several hundred million years of evolution. | |||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_22217.map.gz | 167.8 MB | EMDB map data format | |
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Header (meta data) | emd-22217-v30.xml emd-22217.xml | 7.5 KB 7.5 KB | Display Display | EMDB header |
Images | emd_22217.png | 131 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-22217 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-22217 | HTTPS FTP |
-Validation report
Summary document | emd_22217_validation.pdf.gz | 78.5 KB | Display | EMDB validaton report |
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Full document | emd_22217_full_validation.pdf.gz | 77.6 KB | Display | |
Data in XML | emd_22217_validation.xml.gz | 494 B | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-22217 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-22217 | HTTPS FTP |
-Related structure data
Related structure data | C: citing same article (ref.) |
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Similar structure data | |
EM raw data | EMPIAR-10436 (Title: The CryoEM Structure of Drosophila Flight Muscle Thick Filaments at 7Å Resolution Data size: 12.0 TB / Data #1: Wild Type Drosophila [micrographs - multiframe]) |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_22217.map.gz / Format: CCP4 / Size: 610.4 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Voxel size | X=Y=Z: 2.02 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Sample components
-Entire : Thick filaments
Entire | Name: Thick filaments |
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Components |
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-Supramolecule #1: Thick filaments
Supramolecule | Name: Thick filaments / type: complex / ID: 1 / Parent: 0 |
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Source (natural) | Organism: Drosophila melanogaster (fruit fly) |
Molecular weight | Theoretical: 500 kDa/nm |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | filament |
-Sample preparation
Buffer | pH: 6.8 |
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Grid | Model: Quantifoil |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN |
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Image recording | Film or detector model: DIRECT ELECTRON DE-64 (8k x 8k) / Average electron dose: 26.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
-Image processing
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 7.0 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 149000 |
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Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |