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データを開く
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基本情報
| 登録情報 | ![]() | |||||||||||||||
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| タイトル | Cryo-EM structure of the yeast Inner kinetochore bound to a CENP-A nucleosome. | |||||||||||||||
マップデータ | ||||||||||||||||
試料 |
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キーワード | kinetochore / point centromere / CENP-A nucleosome / topological entrapment / centromeric DNA / CELL CYCLE | |||||||||||||||
| 機能・相同性 | 機能・相同性情報Cbf1-Met4-Met28 complex / positive regulation of sulfate assimilation / regulation of sulfur metabolic process / negative regulation of kinetochore assembly / positive regulation of inositol biosynthetic process / 2-micrometer circle DNA / 2-micrometer plasmid partitioning / negative regulation of meiotic DNA double-strand break formation involved in reciprocal meiotic recombination / COMA complex / maintenance of meiotic sister chromatid cohesion ...Cbf1-Met4-Met28 complex / positive regulation of sulfate assimilation / regulation of sulfur metabolic process / negative regulation of kinetochore assembly / positive regulation of inositol biosynthetic process / 2-micrometer circle DNA / 2-micrometer plasmid partitioning / negative regulation of meiotic DNA double-strand break formation involved in reciprocal meiotic recombination / COMA complex / maintenance of meiotic sister chromatid cohesion / RAVE complex / Iron uptake and transport / : / CBF3 complex / HDMs demethylate histones / regulation of transcription by galactose / : / cellular response to methylmercury / Mis6-Sim4 complex / vacuolar proton-transporting V-type ATPase complex assembly / meiotic sister chromatid segregation / negative regulation of ceramide biosynthetic process / septin ring assembly / establishment of meiotic sister chromatid cohesion / HATs acetylate histones / ascospore formation / RNA polymerase I upstream activating factor complex / attachment of spindle microtubules to kinetochore / Condensation of Prophase Chromosomes / : / : / : / centromeric DNA binding / Assembly of the ORC complex at the origin of replication / HDACs deacetylate histones / CENP-A containing chromatin assembly / regulation of exit from mitosis / protein localization to chromosome, centromeric region / kinetochore assembly / outer kinetochore / condensed chromosome, centromeric region / establishment of mitotic sister chromatid cohesion / protein localization to kinetochore / cellular response to methionine / positive regulation of D-glucose transmembrane transport / spindle pole body / Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks / Oxidative Stress Induced Senescence / vacuolar acidification / exit from mitosis / RMTs methylate histone arginines / protein neddylation / DNA damage tolerance / mitotic intra-S DNA damage checkpoint signaling / mitochondrial fusion / SUMOylation of chromatin organization proteins / silent mating-type cassette heterochromatin formation / DNA binding, bending / SCF ubiquitin ligase complex / FBXL7 down-regulates AURKA during mitotic entry and in early mitosis / mitotic spindle assembly checkpoint signaling / Ubiquitin-Mediated Degradation of Phosphorylated Cdc25A / Orc1 removal from chromatin / SCF-dependent proteasomal ubiquitin-dependent protein catabolic process / RNA Polymerase I Promoter Escape / positive regulation of transcription by RNA polymerase I / nucleolar large rRNA transcription by RNA polymerase I / Estrogen-dependent gene expression / rRNA transcription / Antigen processing: Ubiquitination & Proteasome degradation / mitotic sister chromatid segregation / DNA replication origin binding / chromosome, centromeric region / DNA replication initiation / cullin family protein binding / regulation of protein-containing complex assembly / Ub-specific processing proteases / subtelomeric heterochromatin formation / protein localization to CENP-A containing chromatin / negative regulation of cytoplasmic translation / CENP-A containing nucleosome / endomembrane system / regulation of mitotic cell cycle / meiotic cell cycle / chromosome segregation / G1/S transition of mitotic cell cycle / kinetochore / DNA-binding transcription repressor activity, RNA polymerase II-specific / G2/M transition of mitotic cell cycle / structural constituent of chromatin / peroxisome / nucleosome / mitotic cell cycle / heterochromatin formation / nucleosome assembly / chromatin organization / protein-containing complex assembly / DNA-binding transcription activator activity, RNA polymerase II-specific / sequence-specific DNA binding / ubiquitin-dependent protein catabolic process 類似検索 - 分子機能 | |||||||||||||||
| 生物種 | ![]() | |||||||||||||||
| 手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.7 Å | |||||||||||||||
データ登録者 | Dendooven TD / Zhang Z / Yang J / McLaughlin S / Schwabb J / Scheres S / Yatskevich S / Barford D | |||||||||||||||
| 資金援助 | 英国, ドイツ, 4件
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引用 | ジャーナル: Sci Adv / 年: 2023タイトル: Cryo-EM structure of the complete inner kinetochore of the budding yeast point centromere. 著者: Tom Dendooven / Ziguo Zhang / Jing Yang / Stephen H McLaughlin / Johannes Schwab / Sjors H W Scheres / Stanislau Yatskevich / David Barford / ![]() 要旨: The point centromere of budding yeast specifies assembly of the large kinetochore complex to mediate chromatid segregation. Kinetochores comprise the centromere-associated inner kinetochore (CCAN) ...The point centromere of budding yeast specifies assembly of the large kinetochore complex to mediate chromatid segregation. Kinetochores comprise the centromere-associated inner kinetochore (CCAN) complex and the microtubule-binding outer kinetochore KNL1-MIS12-NDC80 (KMN) network. The budding yeast inner kinetochore also contains the DNA binding centromere-binding factor 1 (CBF1) and CBF3 complexes. We determined the cryo-electron microscopy structure of the yeast inner kinetochore assembled onto the centromere-specific centromere protein A nucleosomes (CENP-A). This revealed a central CENP-A with extensively unwrapped DNA ends. These free DNA duplexes bind two CCAN protomers, one of which entraps DNA topologically, positioned on the centromere DNA element I (CDEI) motif by CBF1. The two CCAN protomers are linked through CBF3 forming an arch-like configuration. With a structural mechanism for how CENP-A can also be linked to KMN involving only CENP-QU, we present a model for inner kinetochore assembly onto a point centromere and how it organizes the outer kinetochore for chromosome attachment to the mitotic spindle. | |||||||||||||||
| 履歴 |
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構造の表示
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ダウンロードとリンク
-EMDBアーカイブ
| マップデータ | emd_17227.map.gz | 96.9 MB | EMDBマップデータ形式 | |
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| ヘッダ (付随情報) | emd-17227-v30.xml emd-17227.xml | 49.7 KB 49.7 KB | 表示 表示 | EMDBヘッダ |
| 画像 | emd_17227.png | 83.3 KB | ||
| Filedesc metadata | emd-17227.cif.gz | 12.1 KB | ||
| その他 | emd_17227_additional_1.map.gz emd_17227_half_map_1.map.gz emd_17227_half_map_2.map.gz | 29.6 MB 131.5 MB 131.5 MB | ||
| アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-17227 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-17227 | HTTPS FTP |
-関連構造データ
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リンク
| EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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| 「今月の分子」の関連する項目 |
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マップ
| ファイル | ダウンロード / ファイル: emd_17227.map.gz / 形式: CCP4 / 大きさ: 166.4 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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| 投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||
| ボクセルのサイズ | X=Y=Z: 1.08 Å | ||||||||||||||||||||||||||||||||||||
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| 対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||
| 詳細 | EMDB XML:
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-添付データ
-追加マップ: #1
| ファイル | emd_17227_additional_1.map | ||||||||||||
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| 密度ヒストグラム |
-ハーフマップ: #2
| ファイル | emd_17227_half_map_1.map | ||||||||||||
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| 密度ヒストグラム |
-ハーフマップ: #1
| ファイル | emd_17227_half_map_2.map | ||||||||||||
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| 投影像・断面図 |
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試料の構成要素
+全体 : A complex of CBF1-CCAN bound to centromeric C0N3 DNA
+超分子 #1: A complex of CBF1-CCAN bound to centromeric C0N3 DNA
+分子 #1: Centromere-binding protein 1
+分子 #2: Inner kinetochore subunit CTF3
+分子 #3: Inner kinetochore subunit MCM22
+分子 #4: Inner kinetochore subunit MCM16
+分子 #5: Inner kinetochore subunit IML3
+分子 #6: Inner kinetochore subunit CHL4
+分子 #7: Centromere DNA-binding protein complex CBF3 subunit C
+分子 #8: Centromere DNA-binding protein complex CBF3 subunit B
+分子 #9: Suppressor of kinetochore protein 1
+分子 #10: Inner kinetochore subunit CTF19
+分子 #11: Inner kinetochore subunit OKP1
+分子 #12: Inner kinetochore subunit MCM21
+分子 #13: Inner kinetochore subunit CNN1
+分子 #14: Inner kinetochore subunit AME1
+分子 #15: Inner kinetochore subunit WIP1
+分子 #16: Inner kinetochore subunit NKP1
+分子 #17: Inner kinetochore subunit NKP2
+分子 #20: Histone H4
+分子 #21: Histone H2B.1
+分子 #22: Histone H3-like centromeric protein CSE4
+分子 #23: Histone H2A.1
+分子 #18: C0N3
+分子 #19: C0N3
-実験情報
-構造解析
| 手法 | クライオ電子顕微鏡法 |
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解析 | 単粒子再構成法 |
| 試料の集合状態 | particle |
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試料調製
| 緩衝液 | pH: 8 |
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| 凍結 | 凍結剤: ETHANE |
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電子顕微鏡法
| 顕微鏡 | FEI TITAN KRIOS |
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| 撮影 | フィルム・検出器のモデル: GATAN K3 BIOQUANTUM (6k x 4k) 平均電子線量: 40.0 e/Å2 |
| 電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
| 電子光学系 | 照射モード: SPOT SCAN / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 2.6 µm / 最小 デフォーカス(公称値): 1.0 µm |
| 実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
ムービー
コントローラー
万見について




キーワード
データ登録者
英国,
ドイツ, 4件
引用




























Z (Sec.)
Y (Row.)
X (Col.)












































Trichoplusia ni (イラクサキンウワバ)
解析
FIELD EMISSION GUN
