+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 1aze | ||||||
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タイトル | NMR STRUCTURE OF THE COMPLEX BETWEEN THE C32S-Y7V MUTANT OF THE NSH3 DOMAIN OF GRB2 WITH A PEPTIDE FROM SOS, 10 STRUCTURES | ||||||
要素 |
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キーワード | COMPLEX (ADAPTOR PROTEIN/PEPTIDE) / COMPLEX (ADAPTOR PROTEIN-PEPTIDE) / SH3 DOMAIN / GUANINE-NUCLEOTIDE RELEASING FACTOR / COMPLEX (ADAPTOR PROTEIN-PEPTIDE) complex | ||||||
機能・相同性 | 機能・相同性情報 : / : / NRAGE signals death through JNK / G alpha (12/13) signalling events / rhabdomere microvillus membrane / guanyl-nucleotide exchange factor activity => GO:0005085 / guanyl-nucleotide exchange factor activity => GO:0005085 / photoreceptor cell fate determination / SOS-mediated signalling / Signaling by SCF-KIT ...: / : / NRAGE signals death through JNK / G alpha (12/13) signalling events / rhabdomere microvillus membrane / guanyl-nucleotide exchange factor activity => GO:0005085 / guanyl-nucleotide exchange factor activity => GO:0005085 / photoreceptor cell fate determination / SOS-mediated signalling / Signaling by SCF-KIT / Regulation of KIT signaling / Signalling to RAS / DAP12 signaling / SHC-related events triggered by IGF1R / Role of LAT2/NTAL/LAB on calcium mobilization / FCERI mediated Ca+2 mobilization / SHC-mediated cascade:FGFR1 / FRS-mediated FGFR1 signaling / SHC-mediated cascade:FGFR2 / FRS-mediated FGFR2 signaling / SHC-mediated cascade:FGFR3 / FRS-mediated FGFR3 signaling / FRS-mediated FGFR4 signaling / SHC-mediated cascade:FGFR4 / Signal attenuation / Insulin receptor signalling cascade / FCERI mediated MAPK activation / SHC1 events in ERBB4 signaling / GRB2 events in EGFR signaling / SHC1 events in EGFR signaling / GRB2 events in ERBB2 signaling / EGFR Transactivation by Gastrin / torso signaling pathway / RAF/MAP kinase cascade / tracheal outgrowth, open tracheal system / R7 cell fate commitment / sevenless signaling pathway / epithelial cell migration, open tracheal system / imaginal disc-derived leg morphogenesis / guanyl-nucleotide exchange factor adaptor activity / Grb2-EGFR complex / branching involved in labyrinthine layer morphogenesis / STAT5 Activation / COP9 signalosome / neurotrophin TRKA receptor binding / Activated NTRK2 signals through PI3K / MET receptor recycling / transmembrane receptor protein tyrosine kinase adaptor activity / Signaling by cytosolic FGFR1 fusion mutants / Interleukin-15 signaling / positive regulation of Ras protein signal transduction / MET activates PTPN11 / axon midline choice point recognition / vesicle membrane / MET activates RAP1 and RAC1 / Costimulation by the CD28 family / Signaling by LTK / CD28 dependent Vav1 pathway / MET activates PI3K/AKT signaling / Signal regulatory protein family interactions / natural killer cell mediated cytotoxicity / Regulation of KIT signaling / epidermal growth factor receptor binding / negative regulation of G1/S transition of mitotic cell cycle / small GTPase-mediated signal transduction / positive regulation of actin filament polymerization / PI-3K cascade:FGFR3 / STAT5 activation downstream of FLT3 ITD mutants / PI-3K cascade:FGFR2 / PI-3K cascade:FGFR4 / endodermal cell differentiation / PI-3K cascade:FGFR1 / positive regulation of Rac protein signal transduction / extrinsic component of cytoplasmic side of plasma membrane / GRB2:SOS provides linkage to MAPK signaling for Integrins / regulation of MAPK cascade / RHOU GTPase cycle / PI3K events in ERBB2 signaling / SOS-mediated signalling / positive regulation of cell size / RET signaling / Activated NTRK3 signals through RAS / Activated NTRK2 signals through RAS / Interleukin-3, Interleukin-5 and GM-CSF signaling / insulin receptor substrate binding / PI3K Cascade / SHC1 events in ERBB4 signaling / Signalling to RAS / RHO GTPases Activate WASPs and WAVEs / signal transduction in response to DNA damage / fibroblast growth factor receptor signaling pathway / SHC-related events triggered by IGF1R / GAB1 signalosome / Activated NTRK2 signals through FRS2 and FRS3 / Role of LAT2/NTAL/LAB on calcium mobilization / Interleukin receptor SHC signaling / Signal attenuation / SHC-mediated cascade:FGFR3 / vascular endothelial growth factor receptor signaling pathway / MET activates RAS signaling 類似検索 - 分子機能 | ||||||
生物種 | Homo sapiens (ヒト) Drosophila melanogaster (キイロショウジョウバエ) | ||||||
手法 | 溶液NMR / SIMULATED ANNEALING ENERGY MINIMIZATION | ||||||
データ登録者 | Vidal, M. / Gincel, E. / Goudreau, N. / Cornille, F. / Parker, F. / Duchesne, M. / Tocque, B. / Garbay, C. / Roques, B.P. | ||||||
引用 | ジャーナル: J.Mol.Biol. / 年: 1999 タイトル: Molecular and cellular analysis of Grb2 SH3 domain mutants: interaction with Sos and dynamin. 著者: Vidal, M. / Goudreau, N. / Cornille, F. / Cussac, D. / Gincel, E. / Garbay, C. #1: ジャーナル: Nat.Struct.Biol. / 年: 1994 タイトル: NMR Structure of the N-Terminal SH3 Domain of Grb2 and its Complex with a Proline-Rich Peptide from SOS 著者: Goudreau, N. / Cornille, F. / Duchesne, M. / Parker, F. / Tocque, B. / Garbay, C. / Roques, B.P. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 1aze.cif.gz | 215.8 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb1aze.ent.gz | 177.3 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 1aze.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/az/1aze ftp://data.pdbj.org/pub/pdb/validation_reports/az/1aze | HTTPS FTP |
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-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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NMR アンサンブル |
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-要素
#1: タンパク質 | 分子量: 6477.309 Da / 分子数: 1 / 断片: N-TERMINAL SH3 DOMAIN, RESIDUES 1 - 55 / 変異: Y7V, C32S / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: GRB2 / 器官: FRUIT / 参照: UniProt: P62993 |
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#2: タンパク質・ペプチド | 分子量: 1173.432 Da / 分子数: 1 / 断片: BINDING SITE IN H-SOS, PEPTIDE VPPPVPPRRR / 由来タイプ: 組換発現 由来: (組換発現) Drosophila melanogaster (キイロショウジョウバエ) 参照: UniProt: P26675 |
-実験情報
-実験
実験 | 手法: 溶液NMR | ||||||||||||||||
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NMR実験 |
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-試料調製
詳細 | 内容: H2O, PHOSPHATE BUFFER 20MM, NACL 100MM |
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試料状態 | イオン強度: NACL 100mM / pH: 6 / 圧: 1 atm / 温度: 293 K |
結晶化 | *PLUS 手法: other / 詳細: NMR |
-NMR測定
NMRスペクトロメーター | タイプ: Bruker AMX600 / 製造業者: Bruker / モデル: AMX600 / 磁場強度: 600 MHz |
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-解析
NMR software |
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精密化 | 手法: SIMULATED ANNEALING ENERGY MINIMIZATION / ソフトェア番号: 1 | ||||||||||||
NMRアンサンブル | コンフォーマー選択の基準: BEST ENERGY, LEAST RESTRAINT VIOLATION 計算したコンフォーマーの数: 50 / 登録したコンフォーマーの数: 10 | ||||||||||||
精密化 | *PLUS | ||||||||||||
溶媒の処理 | *PLUS | ||||||||||||
原子変位パラメータ | *PLUS |