+Open data
-Basic information
Entry | Database: PDB / ID: 1ail | ||||||
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Title | N-TERMINAL FRAGMENT OF NS1 PROTEIN FROM INFLUENZA A VIRUS | ||||||
Components | NONSTRUCTURAL PROTEIN NS1 | ||||||
Keywords | RNA BINDING PROTEIN / RNA-BINDING PROTEIN / NONSTRUCTURAL PROTEIN | ||||||
Function / homology | Function and homology information symbiont-mediated suppression of host cytokine production / symbiont-mediated suppression of host mRNA processing / symbiont-mediated suppression of host PKR/eIFalpha signaling / protein serine/threonine kinase inhibitor activity / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of RIG-I activity / double-stranded RNA binding / host cell cytoplasm / symbiont-mediated suppression of host innate immune response / symbiont-mediated suppression of host type I interferon-mediated signaling pathway / virus-mediated perturbation of host defense response ...symbiont-mediated suppression of host cytokine production / symbiont-mediated suppression of host mRNA processing / symbiont-mediated suppression of host PKR/eIFalpha signaling / protein serine/threonine kinase inhibitor activity / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of RIG-I activity / double-stranded RNA binding / host cell cytoplasm / symbiont-mediated suppression of host innate immune response / symbiont-mediated suppression of host type I interferon-mediated signaling pathway / virus-mediated perturbation of host defense response / host cell nucleus / identical protein binding Similarity search - Function | ||||||
Biological species | Influenza A virus | ||||||
Method | X-RAY DIFFRACTION / MIRAS / Resolution: 1.9 Å | ||||||
Authors | Liu, J. / Lynch, P.A. / Chien, C. / Montelione, G.T. / Krug, R.M. / Berman, H.M. | ||||||
Citation | Journal: Nat.Struct.Biol. / Year: 1997 Title: Crystal structure of the unique RNA-binding domain of the influenza virus NS1 protein. Authors: Liu, J. / Lynch, P.A. / Chien, C.Y. / Montelione, G.T. / Krug, R.M. / Berman, H.M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1ail.cif.gz | 24.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1ail.ent.gz | 16 KB | Display | PDB format |
PDBx/mmJSON format | 1ail.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1ail_validation.pdf.gz | 413.2 KB | Display | wwPDB validaton report |
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Full document | 1ail_full_validation.pdf.gz | 413.2 KB | Display | |
Data in XML | 1ail_validation.xml.gz | 4.9 KB | Display | |
Data in CIF | 1ail_validation.cif.gz | 6.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ai/1ail ftp://data.pdbj.org/pub/pdb/validation_reports/ai/1ail | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 8338.430 Da / Num. of mol.: 1 / Fragment: RNA-BINDING DOMAIN / Source method: isolated from a natural source Source: (natural) Influenza A virus (A/Udorn/307/1972(H3N2)) Genus: Influenzavirus A / Species: Influenza A virus / Strain: A/Udorn/307/72 H3N2 / References: UniProt: P03495 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 4 |
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-Sample preparation
Crystal | Density Matthews: 1.86 Å3/Da / Density % sol: 34 % | ||||||||||||||||||||||||||||||
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Crystal grow | pH: 6.5 Details: NATIVE CRYSTALS WERE GROWN FROM 50MM NAH2PO4, 100MM NACL, 1MM NAN3 AT PH 6.5; MERCURY DERIVATIVE CRYSTAL WAS PREPARED BY SOAKING THE NATIVE CRYSTAL IN 2MM CH3HGCL WITH 10% PEG 6000 AT PH 8.0; ...Details: NATIVE CRYSTALS WERE GROWN FROM 50MM NAH2PO4, 100MM NACL, 1MM NAN3 AT PH 6.5; MERCURY DERIVATIVE CRYSTAL WAS PREPARED BY SOAKING THE NATIVE CRYSTAL IN 2MM CH3HGCL WITH 10% PEG 6000 AT PH 8.0; PLATINUM DERIVATIVE CRYSTAL WAS PREPARED BY SOAKING THE NATIVE CRYSTAL IN 4MM PT(NH3)2CL2 WITH 10% PEG 6000 AT PH 6.5. PH range: 6.5 for native,8.0 for Mg derivative,6.5 for Pt derative | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RUH2R / Wavelength: 1.5418 |
Detector | Type: RIGAKU RAXIS IIC / Detector: IMAGE PLATE / Date: Jan 1, 1996 / Details: MIRRORS |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→20 Å / Num. obs: 5318 / % possible obs: 98.2 % / Observed criterion σ(I): 2 / Redundancy: 3.8 % / Rsym value: 0.065 / Net I/σ(I): 18 |
Reflection shell | Resolution: 1.9→1.94 Å / Redundancy: 3 % / Mean I/σ(I) obs: 4.9 / Rsym value: 0.217 / % possible all: 90.7 |
Reflection | *PLUS Rmerge(I) obs: 0.065 |
-Processing
Software |
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Refinement | Method to determine structure: MIRAS / Resolution: 1.9→20 Å / σ(F): 2
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Refinement step | Cycle: LAST / Resolution: 1.9→20 Å
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Software | *PLUS Name: REFMAC / Classification: refinement | ||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.182 | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 18.2 Å2 | ||||||||||||||||||||
Refine LS restraints | *PLUS
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