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- EMDB-9524: Cryo-EM structure of the activated spliceosome (Bact complex) at ... -

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Basic information

Entry
Database: EMDB / ID: EMD-9524
TitleCryo-EM structure of the activated spliceosome (Bact complex) at 3.5 angstrom resolution
Map dataCryo-EM map of Bact spliceosome at 3.5-angstrom resolution
Sample
  • Complex: Bact spliceosomal complex
    • Protein or peptide: x 38 types
    • RNA: x 5 types
  • Ligand: x 4 types
Function / homology
Function and homology information


maintenance of RNA location / mRNA branch site recognition / U2-type post-mRNA release spliceosomal complex / RES complex / cellular bud site selection / snoRNA splicing / post-mRNA release spliceosomal complex / generation of catalytic spliceosome for first transesterification step / cis assembly of pre-catalytic spliceosome / splicing factor binding ...maintenance of RNA location / mRNA branch site recognition / U2-type post-mRNA release spliceosomal complex / RES complex / cellular bud site selection / snoRNA splicing / post-mRNA release spliceosomal complex / generation of catalytic spliceosome for first transesterification step / cis assembly of pre-catalytic spliceosome / splicing factor binding / U4/U6 snRNP / spliceosome conformational change to release U4 (or U4atac) and U1 (or U11) / 7-methylguanosine cap hypermethylation / Prp19 complex / pICln-Sm protein complex / ATP-dependent activity, acting on RNA / snRNP binding / U2-type catalytic step 1 spliceosome / small nuclear ribonucleoprotein complex / pre-mRNA binding / SMN-Sm protein complex / spliceosomal tri-snRNP complex / U2-type spliceosomal complex / mRNA cis splicing, via spliceosome / poly(U) RNA binding / commitment complex / U2-type prespliceosome assembly / U2-type catalytic step 2 spliceosome / U4 snRNP / U2 snRNP / U1 snRNP / U2-type prespliceosome / precatalytic spliceosome / Formation of TC-NER Pre-Incision Complex / generation of catalytic spliceosome for second transesterification step / spliceosomal complex assembly / DNA replication origin binding / mRNA 5'-splice site recognition / Gap-filling DNA repair synthesis and ligation in TC-NER / protein K63-linked ubiquitination / mRNA 3'-splice site recognition / Dual incision in TC-NER / spliceosomal tri-snRNP complex assembly / DNA replication initiation / U5 snRNA binding / positive regulation of cell cycle / U5 snRNP / mRNA export from nucleus / U2 snRNA binding / U6 snRNA binding / spliceosomal snRNP assembly / pre-mRNA intronic binding / U1 snRNA binding / U4/U6 x U5 tri-snRNP complex / catalytic step 2 spliceosome / RNA splicing / nuclear periphery / positive regulation of RNA splicing / peptidylprolyl isomerase / peptidyl-prolyl cis-trans isomerase activity / helicase activity / spliceosomal complex / RING-type E3 ubiquitin transferase / mRNA splicing, via spliceosome / mRNA processing / ubiquitin-protein transferase activity / metallopeptidase activity / ubiquitin protein ligase activity / nucleic acid binding / RNA helicase activity / RNA helicase / response to xenobiotic stimulus / DNA repair / GTPase activity / mRNA binding / chromatin binding / chromatin / GTP binding / ATP hydrolysis activity / mitochondrion / DNA binding / RNA binding / zinc ion binding / ATP binding / identical protein binding / nucleus / metal ion binding / cytosol / cytoplasm
Similarity search - Function
Pre-mRNA-splicing factor CWC24-like / Ist3-like, RNA recognition motif / : / Bud13 / : / Pre-mRNA-splicing factor of RES complex / cwf21 / Slt11, RNA recognition motif / Pre-mRNA-splicing factor Cwc2, RNA recognition motif / Torus domain ...Pre-mRNA-splicing factor CWC24-like / Ist3-like, RNA recognition motif / : / Bud13 / : / Pre-mRNA-splicing factor of RES complex / cwf21 / Slt11, RNA recognition motif / Pre-mRNA-splicing factor Cwc2, RNA recognition motif / Torus domain / Torus domain / : / mRNA splicing factor SYF2 / SYF2 splicing factor / : / : / mRNA splicing factor Cwf21 domain / cwf21 domain / SF3A2 domain / : / Pre-mRNA-splicing factor SF3a complex subunit 2 (Prp11) / Pre-mRNA-processing factor 17 / SF3B4, RNA recognition motif 1 / : / Zinc finger, C3HC4 type (RING finger) / Pre-mRNA-splicing factor 19 / Pre-mRNA-processing factor 19 / Prp19/Pso4-like / Zinc finger C-x8-C-x5-C-x3-H type (and similar) / Cyclophilin-type peptidyl-prolyl cis-trans isomerase, cyclophilin A-like / : / BUD31/G10-related, conserved site / G10 protein signature 1. / G10 protein signature 2. / Domain of unknown function DUF382 / : / Domain of unknown function (DUF382) / STL11, N-terminal / SKI-interacting protein SKIP, SNW domain / SKI-interacting protein, SKIP / SKIP/SNW domain / Pre-mRNA-splicing factor Cwf15/Cwc15 / Suppressor of forked / Cwf15/Cwc15 cell cycle control protein / Suppressor of forked protein (Suf) / WD repeat Prp46/PLRG1-like / Pre-mRNA-splicing factor Cwc2/Slt11 / G10 protein / Pre-mRNA-splicing factor BUD31 / Zinc-finger of C2H2 type / Pre-mRNA splicing factor component Cdc5p/Cef1, C-terminal / : / : / pre-mRNA splicing factor component / PHF5-like / PHF5-like protein / PSP, proline-rich / PSP / proline-rich domain in spliceosome associated proteins / Splicing factor 3B subunit 5/RDS3 complex subunit 10 / Splicing factor 3B subunit 10 (SF3b10) / Initiation factor eIF-4 gamma, MA3 / MA3 domain / MI domain profile. / Domain in DAP-5, eIF4G, MA-3 and other proteins. / Splicing factor 3B subunit 1-like / Middle domain of eukaryotic initiation factor 4G (eIF4G) / MIF4G-like, type 3 / U-box domain profile. / Modified RING finger domain / Zinc finger, CCCH-type superfamily / U-box domain / PPP2R1A-like HEAT repeat / Brr2, N-terminal helicase PWI domain / : / N-terminal helicase PWI domain / Pre-mRNA-splicing helicase BRR2 plug domain / Sec63 Brl domain / zinc finger / : / Helicase associated domain (HA2), ratchet-like / : / DEAD-box helicase, OB fold / Oligonucleotide/oligosaccharide-binding (OB)-fold / Helicase associated domain (HA2), winged-helix / Helicase-associated domain / Pre-mRNA-splicing factor Syf1-like / Helicase associated domain (HA2) Add an annotation / 116kDa U5 small nuclear ribonucleoprotein component, N-terminal / 116kDa U5 small nuclear ribonucleoprotein component, C-terminal / Snu114, GTP-binding domain / 116 kDa U5 small nuclear ribonucleoprotein component N-terminus / Sec63 domain / : / Sec63 Brl domain / Small nuclear ribonucleoprotein Sm D3 / Small nuclear ribonucleoprotein Sm D2 / Small nuclear ribonucleoprotein E / Small nuclear ribonucleoprotein G / Small nuclear ribonucleoprotein F
Similarity search - Domain/homology
RDS3 complex subunit 10 / Pre-mRNA-splicing factor ATP-dependent RNA helicase-like protein PRP2 / Pre-mRNA-splicing factor BUD31 / Pre-mRNA-processing protein 45 / Pre-mRNA-processing factor 19 / Pre-mRNA-splicing helicase BRR2 / Pre-mRNA-splicing factor 8 / Pre-mRNA-splicing factor SNU114 / Pre-mRNA-splicing factor SLT11 / Small nuclear ribonucleoprotein-associated protein B ...RDS3 complex subunit 10 / Pre-mRNA-splicing factor ATP-dependent RNA helicase-like protein PRP2 / Pre-mRNA-splicing factor BUD31 / Pre-mRNA-processing protein 45 / Pre-mRNA-processing factor 19 / Pre-mRNA-splicing helicase BRR2 / Pre-mRNA-splicing factor 8 / Pre-mRNA-splicing factor SNU114 / Pre-mRNA-splicing factor SLT11 / Small nuclear ribonucleoprotein-associated protein B / Small nuclear ribonucleoprotein G / U2 snRNP component IST3 / Pre-mRNA-processing factor 17 / Small nuclear ribonucleoprotein Sm D3 / Pre-mRNA-splicing factor CWC26 / U2 snRNP component HSH155 / Pre-mRNA-splicing factor SYF2 / Pre-mRNA-splicing factor CWC22 / Pre-mRNA-splicing factor CWC24 / Small nuclear ribonucleoprotein F / Small nuclear ribonucleoprotein Sm D1 / Cold sensitive U2 snRNA suppressor 1 / Peptidyl-prolyl isomerase CWC27 / Pre-mRNA-splicing factor CWC21 / Pre-mRNA-splicing factor CEF1 / Pre-mRNA-splicing factor CWC15 / Pre-mRNA-splicing factor SYF1 / Pre-mRNA-splicing factor RSE1 / Pre-mRNA-splicing factor SNT309 / Small nuclear ribonucleoprotein Sm D2 / Pre-mRNA-splicing factor RDS3 / Pre-mRNA-splicing factor PRP11 / Pre-mRNA leakage protein 1 / Pre-mRNA-splicing factor CWC2 / Pre-mRNA-splicing factor CLF1 / Small nuclear ribonucleoprotein E / Pre-mRNA-splicing factor PRP46 / Protein HSH49
Similarity search - Component
Biological speciesSaccharomyces cerevisiae S288c (yeast) / Baker's yeast (brewer's yeast) / Saccharomyces cerevisiae (brewer's yeast)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.5 Å
AuthorsYan C / Wan R / Bai R / Huang G / Shi Y
CitationJournal: Science / Year: 2016
Title: Structure of a yeast activated spliceosome at 3.5 Å resolution.
Authors: Chuangye Yan / Ruixue Wan / Rui Bai / Gaoxingyu Huang / Yigong Shi /
Abstract: Pre-messenger RNA (pre-mRNA) splicing is carried out by the spliceosome, which undergoes an intricate assembly and activation process. Here, we report an atomic structure of an activated spliceosome ...Pre-messenger RNA (pre-mRNA) splicing is carried out by the spliceosome, which undergoes an intricate assembly and activation process. Here, we report an atomic structure of an activated spliceosome (known as the B(act) complex) from Saccharomyces cerevisiae, determined by cryo-electron microscopy at an average resolution of 3.52 angstroms. The final refined model contains U2 and U5 small nuclear ribonucleoprotein particles (snRNPs), U6 small nuclear RNA (snRNA), nineteen complex (NTC), NTC-related (NTR) protein, and a 71-nucleotide pre-mRNA molecule, which amount to 13,505 amino acids from 38 proteins and a combined molecular mass of about 1.6 megadaltons. The 5' exon is anchored by loop I of U5 snRNA, whereas the 5' splice site (5'SS) and the branch-point sequence (BPS) of the intron are specifically recognized by U6 and U2 snRNA, respectively. Except for coordination of the catalytic metal ions, the RNA elements at the catalytic cavity of Prp8 are mostly primed for catalysis. The catalytic latency is maintained by the SF3b complex, which encircles the BPS, and the splicing factors Cwc24 and Prp11, which shield the 5' exon-5'SS junction. This structure, together with those determined earlier, outlines a molecular framework for the pre-mRNA splicing reaction.
History
DepositionAug 18, 2016-
Header (metadata) releaseSep 21, 2016-
Map releaseSep 21, 2016-
UpdateNov 6, 2019-
Current statusNov 6, 2019Processing site: PDBj / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.0405
  • Imaged by UCSF Chimera
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  • Surface view colored by radius
  • Surface level: 0.0405
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-5gm6
  • Surface level: 0.0405
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_9524.png

Downloads & links

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Map

FileDownload / File: emd_9524.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationCryo-EM map of Bact spliceosome at 3.5-angstrom resolution
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.31 Å/pix.
x 400 pix.
= 522.4 Å		1.31 Å/pix.
x 400 pix.
= 522.4 Å		1.31 Å/pix.
x 400 pix.
= 522.4 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.306 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.0405 / Movie #1: 0.0405
Minimum - Maximum-0.17386426 - 0.2845398
Average (Standard dev.)-0.0000032817325 (±0.0088949995)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions400400400
Spacing400400400
CellA=B=C: 522.4 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.3061.3061.306
M x/y/z400400400
origin x/y/z0.0000.0000.000
length x/y/z522.400522.400522.400
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS400400400
D min/max/mean-0.1740.285-0.000

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Supplemental data

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Sample components

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Entire : Bact spliceosomal complex

EntireName: Bact spliceosomal complex
Components
  • Complex: Bact spliceosomal complex
    • Protein or peptide: Pre-mRNA-splicing factor 8
    • Protein or peptide: Pre-mRNA-splicing helicase BRR2
    • Protein or peptide: Pre-mRNA-splicing factor SNU114
    • RNA: Saccharomyces cerevisiae strain CDRDR_sf_H chromosome VII sequence
    • RNA: Saccharomyces cerevisiae strain T.52_2H chromosome XII sequence
    • Protein or peptide: Pre-mRNA-splicing factor RSE1
    • Protein or peptide: Cold sensitive U2 snRNA suppressor 1
    • Protein or peptide: Pre-mRNA-splicing factor PRP11
    • Protein or peptide: Pre-mRNA-splicing factor RDS3
    • Protein or peptide: RDS3 complex subunit 10
    • RNA: U2 snRNA
    • RNA: Pre-mRNA
    • RNA: Pre-mRNA
    • Protein or peptide: Pre-mRNA-splicing factor PRP46
    • Protein or peptide: Pre-mRNA-processing protein 45
    • Protein or peptide: Pre-mRNA-splicing factor SLT11
    • Protein or peptide: Pre-mRNA-splicing factor CWC2
    • Protein or peptide: Pre-mRNA-splicing factor CWC15
    • Protein or peptide: Pre-mRNA-splicing factor BUD31
    • Protein or peptide: Pre-mRNA leakage protein 1
    • Protein or peptide: U2 snRNP component IST3
    • Protein or peptide: Pre-mRNA-splicing factor CWC26
    • Protein or peptide: Pre-mRNA-splicing factor ATP-dependent RNA helicase-like protein PRP2
    • Protein or peptide: Pre-mRNA-splicing factor CWC22
    • Protein or peptide: Pre-mRNA-splicing factor CWC24
    • Protein or peptide: Peptidyl-prolyl isomerase CWC27
    • Protein or peptide: Pre-mRNA-splicing factor CEF1
    • Protein or peptide: U2 snRNP component HSH155
    • Protein or peptide: Pre-mRNA-splicing factor CLF1
    • Protein or peptide: Pre-mRNA-splicing factor CWC21
    • Protein or peptide: Pre-mRNA-splicing factor SYF1
    • Protein or peptide: Protein HSH49
    • Protein or peptide: Pre-mRNA-splicing factor SYF2
    • Protein or peptide: Pre-mRNA-processing factor 19
    • Protein or peptide: Pre-mRNA-splicing factor SNT309
    • Protein or peptide: Pre-mRNA-processing factor 17
    • Protein or peptide: Small nuclear ribonucleoprotein-associated protein B
    • Protein or peptide: Small nuclear ribonucleoprotein E
    • Protein or peptide: Small nuclear ribonucleoprotein F
    • Protein or peptide: Small nuclear ribonucleoprotein G
    • Protein or peptide: Small nuclear ribonucleoprotein Sm D3
    • Protein or peptide: Small nuclear ribonucleoprotein Sm D1
    • Protein or peptide: Small nuclear ribonucleoprotein Sm D2
  • Ligand: GUANOSINE-5'-TRIPHOSPHATE
  • Ligand: MAGNESIUM ION
  • Ligand: ZINC ION
  • Ligand: ADENOSINE-5'-DIPHOSPHATE

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Supramolecule #1: Bact spliceosomal complex

SupramoleculeName: Bact spliceosomal complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#43
Source (natural)Organism: Saccharomyces cerevisiae S288c (yeast)
Molecular weightTheoretical: 2.0 MDa

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Macromolecule #1: Pre-mRNA-splicing factor 8

MacromoleculeName: Pre-mRNA-splicing factor 8 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 265.949156 KDa
SequenceString: AYTPKAEMPP EHLRKIINTH SDMASKMYNT DKKAFLGALK YLPHAILKLL ENMPHPWEQA KEVKVLYHTS GAITFVNETP RVIEPVYTA QWSATWIAMR REKRDRTHFK RMRFPPFDDD EPPLSYEQHI ENIEPLDPIN LPLDSQDDEY VKDWLYDSRP L EEDSKKVN ...String:
AYTPKAEMPP EHLRKIINTH SDMASKMYNT DKKAFLGALK YLPHAILKLL ENMPHPWEQA KEVKVLYHTS GAITFVNETP RVIEPVYTA QWSATWIAMR REKRDRTHFK RMRFPPFDDD EPPLSYEQHI ENIEPLDPIN LPLDSQDDEY VKDWLYDSRP L EEDSKKVN GTSYKKWSFD LPEMSNLYRL STPLRDEVTD KNYYYLFDKK SFFNGKALNN AIPGGPKFEP LYPREEEEDY NE FNSIDRV IFRVPIRSEY KVAFPHLYNS RPRSVRIPWY NNPVSCIIQN DEEYDTPALF FDPSLNPIPH FIDNNSSLNV SNT KENGDF TLPEDFAPLL AEEEELILPN TKDAMSLYHS PFPFNRTKGK MVRAQDVALA KKWFLQHPDE EYPVKVKVSY QKLL KNYVL NELHPTLPTN HNKTKLLKSL KNTKYFQQTT IDWVEAGLQL CRQGHNMLNL LIHRKGLTYL HLDYNFNLKP TKTLT TKER KKSRLGNSFH LMRELLKMMK LIVDTHVQFR LGNVDAFQLA DGIHYILNHI GQLTGIYRYK YKVMHQIRAC KDLKHI IYY KFNKNLGKGP GCGFWQPAWR VWLNFLRGTI PLLERYIGNL ITRQFEGRSN EIVKTTTKQR LDAYYDLELR NSVMDDI LE MMPESIRQKK ARTILQHLSE AWRCWKANIP WDVPGMPAPI KKIIERYIKS KADAWVSAAH YNRERIKRGA HVEKTMVK K NLGRLTRLWI KNEQERQRQI QKNGPEITPE EATTIFSVMV EWLESRSFSP IPFPPLTYKN DTKILVLALE DLKDVYASK VRLNASEREE LALIEEAYDN PHDTLNRIKK YLLTQRVFKP VDITMMENYQ NISPVYSVDP LEKITDAYLD QYLWYEADQR KLFPNWIKP SDSEIPPLLV YKWTQGINNL SEIWDVSRGQ SAVLLETTLG EMAEKIDFTL LNRLLRLIVD PNIADYITAK N NVVINFKD MSHVNKYGLI RGLKFASFIF QYYGLVIDLL LLGQERATDL AGPANNPNEF MQFKSKEVEK AHPIRLYTRY LD RIYMLFH FEEDEGEELT DEYLAENPDP NFENSIGYNN RKCWPKDSRM RLIRQDVNLG RAVFWEIQSR VPTSLTSIKW ENA FVSVYS KNNPNLLFSM CGFEVRILPR QRMEEVVSND EGVWDLVDER TKQRTAKAYL KVSEEEIKKF DSRIRGILMA SGST TFTKV AAKWNTSLIS LFTYFREAIV ATEPLLDILV KGETRIQNRV KLGLNSKMPT RFPPAVFYTP KELGGLGMIS ASHIL IPAS DLSWSKQTDT GITHFRAGMT HEDEKLIPTI FRYITTWENE FLDSQRVWAE YATKRQEAIQ QNRRLAFEEL EGSWDR GIP RISTLFQRDR HTLAYDRGHR IRREFKQYSL ERNSPFWWTN SHHDGKLWNL NAYRTDVIQA LGGIETILEH TLFKGTG FN SWEGLFWEKA SGFEDSMQFK KLTHAQRTGL SQIPNRRFTL WWSPTINRAN VYVGFLVQLD LTGIFLHGKI PTLKISLI Q IFRAHLWQKI HESIVFDICQ ILDGELDVLQ IESVTKETVH PRKSYKMNSS AADITMESVH EWEVSKPSLL HETNDSFKG LITNKMWFDV QLRYGDYDSH DISRYVRAKF LDYTTDNVSM YPSPTGVMIG IDLAYNMYDA YGNWFNGLKP LIQNSMRTIM KANPALYVL RERIRKGLQI YQSSVQEPFL NSSNYAELFN NDIKLFVDDT NVYRVTVHKT FEGNVATKAI NGCIFTLNPK T GHLFLKII HTSVWAGQKR LSQLAKWKTA EEVSALVRSL PKEEQPKQII VTRKAMLDPL EVHMLDFPNI AIRPTELRLP FS AAMSIDK LSDVVMKATE PQMVLFNIYD DWLDRISSYT AFSRLTLLLR ALKTNEESAK MILLSDPTIT IKSYHLWPSF TDE QWITIE SQMRDLILTE YGRKYNVNIS ALTQTEIKDI ILGQNIKAPS VKRQKMAELE AARSEKQNDE EAAGASTVMK TKTI NAQGE EIVVVASADY ESQTFSSKNE WRKSAIANTL LYLRLKNIYV SADDFVEEQN VYVLPKNLLK KFIEISDVKI QVAAF IYGM SAKDHPKVKE IKTVVLVPQL GHVGSVQISN IPDIGDLPDT EGLELLGWIH TQTEELKFMA ASEVATHSKL FADKKR DCI DISIFSTPGS VSLSAYNLTD EGYQWGEENK DIMNVLSEGF EPTFSTHAQL LLSDRITGNF IIPSGNVWNY TFMGTAF NQ EGDYNFKYGI PLEFYNEMHR PVHFLQFSEL AGDEELEAEQ IDVFS

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Macromolecule #2: Pre-mRNA-splicing helicase BRR2

MacromoleculeName: Pre-mRNA-splicing helicase BRR2 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA helicase
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 246.470266 KDa
SequenceString: MTEHETKDKA KKIREIYRYD EMSNKVLKVD KRFMNTSQNP QRDAEISQPK SMSGRISAKD MGQGLCNNIN KGLKENDVAV EKTGKSASL KKIQQHNTIL NSSSDFRLHY YPKDPSNVET YEQILQWVTE VLGNDIPHDL IIGTADIFIR QLKENEENED G NIEERKEK ...String:
MTEHETKDKA KKIREIYRYD EMSNKVLKVD KRFMNTSQNP QRDAEISQPK SMSGRISAKD MGQGLCNNIN KGLKENDVAV EKTGKSASL KKIQQHNTIL NSSSDFRLHY YPKDPSNVET YEQILQWVTE VLGNDIPHDL IIGTADIFIR QLKENEENED G NIEERKEK IQHELGINID SLKFNELVKL MKNITDYETH PDNSNKQAVA ILADDEKSDE EEVTEMSNNA NVLGGEINDN ED DDEEYDY NDVEVNSKKK NKRALPNIEN DIIKLSDSKT SNIESVPIYS IDEFFLQRKL RSELGYKDTS VIQDLSEKIL NDI ETLEHN PVALEQKLVD LLKFENISLA EFILKNRSTI FWGIRLAKST ENEIPNLIEK MVAKGLNDLV EQYKFRETTH SKRE LDSGD DQPQSSEAKR TKFSNPAIPP VIDLEKIKFD ESSKLMTVTK VSLPEGSFKR VKPQYDEIHI PAPSKPVIDY ELKEI TSLP DWCQEAFPSS ETTSLNPIQS KVFHAAFEGD SNMLICAPTG SGKTNIALLT VLKALSHHYN PKTKKLNLSA FKIVYI APL KALVQEQVRE FQRRLAFLGI KVAELTGDSR LSRKQIDETQ VLVSTPEKWD ITTRNSNNLA IVELVRLLII DEIHLLH DD RGPVLESIVA RTFWASKYGQ EYPRIIGLSA TLPNYEDVGR FLRVPKEGLF YFDSSFRPCP LSQQFCGIKE RNSLKKLK A MNDACYEKVL ESINEGNQII VFVHSRKETS RTATWLKNKF AEENITHKLT KNDAGSKQIL KTEAANVLDP SLRKLIESG IGTHHAGLTR SDRSLSEDLF ADGLLQVLVC TATLAWGVNL PAHTVIIKGT DVYSPEKGSW EQLSPQDVLQ MLGRAGRPRY DTFGEGIII TDQSNVQYYL SVLNQQLPIE SQFVSKLVDN LNAEVVAGNI KCRNDAVNWL AYTYLYVRML ASPMLYKVPD I SSDGQLKK FRESLVHSAL CILKEQELVL YDAENDVIEA TDLGNIASSF YINHASMDVY NRELDEHTTQ IDLFRIFSMS EE FKYVSVR YEEKRELKQL LEKAPIPIRE DIDDPLAKVN VLLQSYFSQL KFEGFALNSD IVFIHQNAGR LLRAMFEICL KRG WGHPTR MLLNLCKSAT TKMWPTNCPL RQFKTCPVEV IKRLEASTVP WGDYLQLETP AEVGRAIRSE KYGKQVYDLL KRFP KMSVT CNAQPITRSV MRFNIEIIAD WIWDMNVHGS LEPFLLMLED TDGDSILYYD VLFITPDIVG HEFTLSFTYE LKQHN QNNL PPNFFLTLIS ENWWHSEFEI PVSFNGFKLP KKFPPPTPLL ENISISTSEL GNDDFSEVFE FKTFNKIQSQ VFESLY NSN DSVFVGSGKG TGKTAMAELA LLNHWRQNKG RAVYINPSGE KIDFLLSDWN KRFSHLAGGK IINKLGNDPS LNLKLLA KS HVLLATPVQF ELLSRRWRQR KNIQSLELMI YDDAHEISQG VYGAVYETLI SRMIFIATQL EKKIRFVCLS NCLANARD F GEWAGMTKSN IYNFSPSERI EPLEINIQSF KDVEHISFNF SMLQMAFEAS AAAAGNRNSS SVFLPSRKDC MEVASAFMK FSKAIEWDML NVEEEQIVPY IEKLTDGHLR APLKHGVGIL YKGMASNDER IVKRLYEYGA VSVLLISKDC SAFACKTDEV IILGTNLYD GAEHKYMPYT INELLEMVGL ASGNDSMAGK VLILTSHNMK AYYKKFLIEP LPTESYLQYI IHDTLNNEIA N SIIQSKQD CVDWFTYSYF YRRIHVNPSY YGVRDTSPHG ISVFLSNLVE TCLNDLVESS FIEIDDTEAE VTAEVNGGDD EA TEIISTL SNGLIASHYG VSFFTIQSFV SSLSNTSTLK NMLYVLSTAV EFESVPLRKG DRALLVKLSK RLPLRFPEHT SSG SVSFKV FLLLQAYFSR LELPVDFQND LKDILEKVVP LINVVVDILS ANGYLNATTA MDLAQMLIQG VWDVDNPLRQ IPHF NNKIL EKCKEINVET VYDIMALEDE ERDEILTLTD SQLAQVAAFV NNYPNVELTY SLNNSDSLIS GVKQKITIQL TRDVE PENL QVTSEKYPFD KLESWWLVLG EVSKKELYAI KKVTLNKETQ QYELEFDTPT SGKHNLTIWC VCDSYLDADK ELSFEI NVK

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Macromolecule #3: Pre-mRNA-splicing factor SNU114

MacromoleculeName: Pre-mRNA-splicing factor SNU114 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 114.174008 KDa
SequenceString: MEGDDLFDEF GNLIGVDPFD SDEEESVLDE QEQYQTNTFE GSGNNNEIES RQLTSLGSKK ELGISLEHPY GKEVEVLMET KNTQSPQTP LVEPVTERTK LQEHTIFTQL KKNIPKTRYN RDYMLSMANI PERIINVGVI GPLHSGKTSL MDLLVIDSHK R IPDMSKNV ...String:
MEGDDLFDEF GNLIGVDPFD SDEEESVLDE QEQYQTNTFE GSGNNNEIES RQLTSLGSKK ELGISLEHPY GKEVEVLMET KNTQSPQTP LVEPVTERTK LQEHTIFTQL KKNIPKTRYN RDYMLSMANI PERIINVGVI GPLHSGKTSL MDLLVIDSHK R IPDMSKNV ELGWKPLRYL DNLKQEIDRG LSIKLNGSTL LCTDLESKSR MINFLDAPGH VNFMDETAVA LAASDLVLIV ID VVEGVTF VVEQLIKQSI KNNVAMCFVI NKLDRLILDL KLPPMDAYLK LNHIIANINS FTKGNVFSPI DNNIIFASTK LGF TFTIKE FVSYYYAHSI PSSKIDDFTT RLWGSVYYHK GNFRTKPFEN VEKYPTFVEF ILIPLYKIFS YALSMEKDKL KNLL RSNFR VNLSQEALQY DPQPFLKHVL QLIFRQQTGL VDAITRCYQP FELFDNKTAH LSIPGKSTPE GTLWAHVLKT VDYGG AEWS LVRIYSGLLK RGDTVRILDT SQSESRQKRQ LHDISKTETS NEDEDEDDET PSCEVEEIGL LGGRYVYPVH EAHKGQ IVL IKGISSAYIK SATLYSVKSK EDMKQLKFFK PLDYITEAVF KIVLQPLLPR ELPKLLDALN KISKYYPGVI IKVEESG EH VILGNGELYM DCLLYDLRAS YAKIEIKISD PLTVFSESCS NESFASIPVS NSISRLGEEN LPGLSISVAA EPMDSKMI Q DLSRNTLGKG QNCLDIDGIM DNPRKLSKIL RTEYGWDSLA SRNVWSFYNG NVLINDTLPD EISPELLSKY KEQIIQGFY WAVKEGPLAE EPIYGVQYKL LSISVPSDVN IDVMKSQIIP LMKKACYVGL LTAIPILLEP IYEVDITVHA PLLPIVEELM KKRRGSRIY KTIKVAGTPL LEVRGQVPVI ESAGFETDLR LSTNGLGMCQ LYFWHKIWRK VPGDVLDKDA FIPKLKPAPI N SLSRDFVM KTRRRKGIST GGFMSNDGPT LEKYISAELY AQLRENGLVP

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Macromolecule #6: Pre-mRNA-splicing factor RSE1

MacromoleculeName: Pre-mRNA-splicing factor RSE1 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 153.956781 KDa
SequenceString: MWGGGKMAVV SLSPHTAKMR KLFGQASTTM AYDGLKREAE RRTRSDHNIT MVAKDDELYL YHLTLKKQTN FVHSCIGHFV DLEAGSKRE QSQLCVATET HLELYDTADG ELKLIAKFQN LFATITSMKS LDLPHSGSRA KASNWPTFLA LTSDSGNLSI V QIIMHAGA ...String:
MWGGGKMAVV SLSPHTAKMR KLFGQASTTM AYDGLKREAE RRTRSDHNIT MVAKDDELYL YHLTLKKQTN FVHSCIGHFV DLEAGSKRE QSQLCVATET HLELYDTADG ELKLIAKFQN LFATITSMKS LDLPHSGSRA KASNWPTFLA LTSDSGNLSI V QIIMHAGA LRLKTLVNQP LTRTTLRRVS PISYMEIDPN GRCIILSSVE QNKLCFLVDY AQKLRISSPL EIIRPHMVTL DM AVVDVNF NNPCFVTLEI DNAATQLSVH LIFYVLELGL NHIVKKADYL VNPSANFVLS LPDLSRYNIT TSLSDNNYDA DYD TLFNPF VVIGFENHIL VKDMNGFFSL KVEIPKRSIT NSRHKNVTII SGIVQKLKND FFVLLQSNHG DLFKLTVSPD TNDR NRPLV QLSYFDTIQN SHQLHIFKNG YLFALSEMNN NFLFQFEKLG VEKNDFSNVL TSKDPNKSLV FEPSIKLQNL SILSQ QLNL NPSIKSQIVS DSPLSIATKH FTNNKIITLT NAVNYSNLIS TSLPPNATKL WLIPDPATTG DNNTLLFITF PKKTMI LQI DNESMEELTP DEATRSAFKL SQDTTIHTCL MGSHSIIQVC TAELRHIVPT GKSRYSNKLT WVPPAGIRIV CATSSKT QL IISLSNYELV YFKIDVSSDS LIELTTHPEL DTMPSKVAIV QDTQHADLLA IADNEGMIKI MSLKDQKEDF LTVISLQL V SEKISDMIMV RDSSIGQLNL HVGLENGVYM KFHIGDVDGS FTDIKRRFLG LKPVSLSYLR EISVSLNNEE EEEEEEDDD DEKEEEEINS SGAKWMSCVV CHSSSTWVSY TWKNVWTIRQ LKDQNMLSCS KFVNADVAIN GVCSISSSGR LNIGRVSNFP TLDNWFHVH ESSVNKQENG GGDESNEEEE DEMEEEMEML QISTFRPRTI LSFPNNPKSI LFIDNHSGKK QCRISLQIDG E CLKFGSSD HLYKILDDID CVSAAIIDFT RQADHLIICA GDKRLLTYKI LVNKDKLSFD IELLHQTEII SPIHAMLKFK NF LLTAMGS TIVLYGLGKK QLLRRSVTQT PVSITKIVSM HQWNYERLAV GDIHESVTLF IWDPAGNVFI PYVDDSVKRH VTV LKFLDE ATVIGADRYG NAWTLRSPPE CEKIMSNHDP SELSNGAIKY PLDVITLQQK LPNTYDCKFK FQLLNHFFVN DIIT DFHIL DSLSNSDRPG CIYMGLQGTV GCFIPLLSKG NVFMMGNIEN IMAEADDTFY LDYESRKKNN NMRKEDDEEE SGSVV LQGR HGIEDEIICE GSCSILGRDH QEYRSYYAPV RKVIDGDLCE NFLRLSLNEQ EFLAKNLKSV QVEDIIQTIN EVRTNY M

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Macromolecule #7: Cold sensitive U2 snRNA suppressor 1

MacromoleculeName: Cold sensitive U2 snRNA suppressor 1 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 50.339879 KDa
SequenceString: MARTKSRKRS GNNQNKNASV VNNKAEIAAM IDARRLEQKK KGGVTNSKGK TNKVVDAKLE KEFKDVLQRF QVQENDTPKE ITKDEKNNH VVIVEKNPVM NRKHTAEDEL EDTPSDGIEE HLSARKRRKT EKPSLSQLKS QVPYPQIIEW YDCDARYPGL L ASIKCTKN ...String:
MARTKSRKRS GNNQNKNASV VNNKAEIAAM IDARRLEQKK KGGVTNSKGK TNKVVDAKLE KEFKDVLQRF QVQENDTPKE ITKDEKNNH VVIVEKNPVM NRKHTAEDEL EDTPSDGIEE HLSARKRRKT EKPSLSQLKS QVPYPQIIEW YDCDARYPGL L ASIKCTKN VIPVPSHWQS KKEYLSGRSL LGKRPFELPD IIKKTNIEQM RSTLPQSGLD GQDEKSLKEA SRARVQPKMG AL DLDYKKL HDVFFKIGAN WKPDHLLCFG DVYYENRNLF EETNWKRMVD HKRPGRISQE LRAIMNLPEG QLPPWCMKMK DIG LPTGYP DLKIAGLNWD ITNLKGDVYG KIIPNHHSRS KKQGRNYFGA LISFETPEFE NSKEDTQANA ENGRQDDKID DEVE HKLDH FQEDISEVTS AEEKLERNEE ESEKQLYTVL K

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Macromolecule #8: Pre-mRNA-splicing factor PRP11

MacromoleculeName: Pre-mRNA-splicing factor PRP11 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 29.962809 KDa
SequenceString: MNYLEGVGSK KGGGGIASES QFNLQRRKEV ESLLSKGENV PYTFQDEKDD QVRSNPYIYK NHSGKLVCKL CNTMHMSWSS VERHLGGKK HGLNVLRRGI SIEKSSLGRE GQTTHDFRQQ QKIIEAKQSL KNNGTIPVCK IATVKNPKNG SVGLAIQVNY S SEVKENSV ...String:
MNYLEGVGSK KGGGGIASES QFNLQRRKEV ESLLSKGENV PYTFQDEKDD QVRSNPYIYK NHSGKLVCKL CNTMHMSWSS VERHLGGKK HGLNVLRRGI SIEKSSLGRE GQTTHDFRQQ QKIIEAKQSL KNNGTIPVCK IATVKNPKNG SVGLAIQVNY S SEVKENSV DSDDKAKVPP LIRIVSGLEL SDTKQKGKKF LVIAYEPFEN IAIELPPNEI LFSENNDMDN NNDGVDELNK KC TFWDAIS KLYYVQFFFK QAEQEQADV

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Macromolecule #9: Pre-mRNA-splicing factor RDS3

MacromoleculeName: Pre-mRNA-splicing factor RDS3 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 12.283573 KDa
SequenceString:
MSRHQFDLIM CLKQPGVQTG LLCEKCDGKC PICDSYVRPK RKVRVCENCS FGKQAKNCII CNLNVGVNDA FYCWECCRLG KDKDGCPRI LNLGSNRLDR HFEKKKKV

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Macromolecule #10: RDS3 complex subunit 10

MacromoleculeName: RDS3 complex subunit 10 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 10.045401 KDa
SequenceString:
MAEKQRQLKL QKIYKQKYIG LGDESTTREQ WQRNVRNDTL NTLQGHSASL EYVSLSRGDL SIRDTRIHLL KSMSPGYKAY LREER

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Macromolecule #14: Pre-mRNA-splicing factor PRP46

MacromoleculeName: Pre-mRNA-splicing factor PRP46 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 50.771289 KDa
SequenceString: MDGNDHKVEN LGDVDKFYSR IRWNNQFSYM ATLPPHLQSE MEGQKSLLMR YDTYRKESSS FSGEGKKVTL QHVPTDFSEA SQAVISKKD HDTHASAFVN KIFQPEVAEE LIVNRYEKLL SQRPEWHAPW KLSRVINGHL GWVRCVAIDP VDNEWFITGS N DTTMKVWD ...String:
MDGNDHKVEN LGDVDKFYSR IRWNNQFSYM ATLPPHLQSE MEGQKSLLMR YDTYRKESSS FSGEGKKVTL QHVPTDFSEA SQAVISKKD HDTHASAFVN KIFQPEVAEE LIVNRYEKLL SQRPEWHAPW KLSRVINGHL GWVRCVAIDP VDNEWFITGS N DTTMKVWD LATGKLKTTL AGHVMTVRDV AVSDRHPYLF SVSEDKTVKC WDLEKNQIIR DYYGHLSGVR TVSIHPTLDL IA TAGRDSV IKLWDMRTRI PVITLVGHKG PINQVQCTPV DPQVVSSSTD ATVRLWDVVA GKTMKVLTHH KRSVRATALH PKE FSVASA CTDDIRSWGL AEGSLLTNFE SEKTGIINTL SINQDDVLFA GGDNGVLSFY DYKSGHKYQS LATREMVGSL EGER SVLCS TFDKTGLRLI TGEADKSIKI WKQDETATKE SEPGLAWNPN LSAKRF

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Macromolecule #15: Pre-mRNA-processing protein 45

MacromoleculeName: Pre-mRNA-processing protein 45 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 42.548727 KDa
SequenceString: MFSNRLPPPK HSQGRVSTAL SSDRVEPAIL TDQIAKNVKL DDFIPKRQSN FELSVPLPTK AEIQECTART KSYIQRLVNA KLANSNNRA SSRYVTETHQ APANLLLNNS HHIEVVSKQM DPLLPRFVGK KARKVVAPTE NDEVVPVLHM DGSNDRGEAD P NEWKIPAA ...String:
MFSNRLPPPK HSQGRVSTAL SSDRVEPAIL TDQIAKNVKL DDFIPKRQSN FELSVPLPTK AEIQECTART KSYIQRLVNA KLANSNNRA SSRYVTETHQ APANLLLNNS HHIEVVSKQM DPLLPRFVGK KARKVVAPTE NDEVVPVLHM DGSNDRGEAD P NEWKIPAA VSNWKNPNGY TVALERRVGK ALDNENNTIN DGFMKLSEAL ENADKKARQE IRSKMELKRL AMEQEMLAKE SK LKELSQR ARYHNGTPQT GAIVKPKKQT STVARLKELA YSQGRDVSEK IILGAAKRSE QPDLQYDSRF FTRGANASAK RHE DQVYDN PLFVQQDIES IYKTNYEKLD EAVNVKSEGA SGSHGPIQFT KAESDDKSDN YGA

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Macromolecule #16: Pre-mRNA-splicing factor SLT11

MacromoleculeName: Pre-mRNA-splicing factor SLT11 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 40.98859 KDa
SequenceString: MNDEINEPPP NICEQCLGDE ANIRMTKIPQ GSECKICTLP FTLYHFKTSK RSNNIIKTLI CVRCATQRNI CQCCMLDSRW HIPIQLRDH LISLVNEENV MTEEAKNDMM KRFLSLKNVK LGGAQITSDP SEADNIVDKL KNILLRATSD GPSTPLIKNT T ALYKNEKG ...String:
MNDEINEPPP NICEQCLGDE ANIRMTKIPQ GSECKICTLP FTLYHFKTSK RSNNIIKTLI CVRCATQRNI CQCCMLDSRW HIPIQLRDH LISLVNEENV MTEEAKNDMM KRFLSLKNVK LGGAQITSDP SEADNIVDKL KNILLRATSD GPSTPLIKNT T ALYKNEKG ANEVKNLEKY ASVDISHILK KLPLNESFLK NPSTKSFFLY NIDASIPEWK ITDTVSQLLG IKKWKDGNSL SL IVNHKAK CGGLRFQSSE LGERFVSKIS ETLVTPKGLK RGVLLIDRFR IFIIPWSSGF SAASFGTNTA ENIKLSLSLN KLI QLELGL SFPTKSTDNA KNDKKKTSKK VHKDRSKKSK PRANKLTI

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Macromolecule #17: Pre-mRNA-splicing factor CWC2

MacromoleculeName: Pre-mRNA-splicing factor CWC2 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 38.486562 KDa
SequenceString: MTSWRDKSAK VQVKESELPS SIPAQTGLTF NIWYNKWSQG FAGNTRFVSP FALQPQLHSG KTRGDNDGQL FFCLFFAKGM CCLGPKCEY LHHIPDEEDI GKLALRTEVL DCFGREKFAD YREDMGGIGS FRKKNKTLYV GGIDGALNSK HLKPAQIESR I RFVFSRLG ...String:
MTSWRDKSAK VQVKESELPS SIPAQTGLTF NIWYNKWSQG FAGNTRFVSP FALQPQLHSG KTRGDNDGQL FFCLFFAKGM CCLGPKCEY LHHIPDEEDI GKLALRTEVL DCFGREKFAD YREDMGGIGS FRKKNKTLYV GGIDGALNSK HLKPAQIESR I RFVFSRLG DIDRIRYVES KNCGFVKFKY QANAEFAKEA MSNQTLLLPS DKEWDDRREG TGLLVKWANE DPDPAAQKRL QE ELKLESL NMMVHLINNN TNSAGTEVNN KNNERLDRTF PEASVDNVKK RLLPLDNGME SDDFIEKLKK VKKNISRENI SSK PSVGKL GGPLLDYLSS DED

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Macromolecule #18: Pre-mRNA-splicing factor CWC15

MacromoleculeName: Pre-mRNA-splicing factor CWC15 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 19.975195 KDa
SequenceString:
MTTSHRPQLE ARSGAKAAAY TPTGIEHARL LPGHTTLKYR KFKEEENLRA NCAQEDRSND KSLEEAVMNE EKQDVVGSGN LQETRSEKD QKDSLQELLV TQKNKVEDKA ELEGNEQLKG GNSSRRSWRK GTAFGRHKVT KETNIKEHAT KKSASGYIND M TKSEYHQE FLHKHVR

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Macromolecule #19: Pre-mRNA-splicing factor BUD31

MacromoleculeName: Pre-mRNA-splicing factor BUD31 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 18.484502 KDa
SequenceString:
MPRIKTRRSK PAPDGFEKIK PTLTDFEIQL RDAQKDKSSK LAAKSNEQLW EIMQLHHQRS RYIYTLYYKR KAISKDLYDW LIKEKYADK LLIAKWRKTG YEKLCCLRCI QKNETNNGST CICRVPRAQL EEEARKKGTQ VSFHQCVHCG CRGCASTD

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Macromolecule #20: Pre-mRNA leakage protein 1

MacromoleculeName: Pre-mRNA leakage protein 1 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 24.100123 KDa
SequenceString: MFHRRKRPYN TRNYGHDDKK FKSQYIDIMP DFSPSGLLEL ESNNKEGIAL KHVEPQDAIS PDNYMDMLGL EARDRTMYEL VIYRKNDKD KGPWKRYDLN GRSCYLVGRE LGHSLDTDLD DRTEIVVADI GIPEETSSKQ HCVIQFRNVR GILKCYVMDL D SSNGTCLN ...String:
MFHRRKRPYN TRNYGHDDKK FKSQYIDIMP DFSPSGLLEL ESNNKEGIAL KHVEPQDAIS PDNYMDMLGL EARDRTMYEL VIYRKNDKD KGPWKRYDLN GRSCYLVGRE LGHSLDTDLD DRTEIVVADI GIPEETSSKQ HCVIQFRNVR GILKCYVMDL D SSNGTCLN NVVIPGARYI ELRSGDVLTL SEFEEDNDYE LIFMNVHHH

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Macromolecule #21: U2 snRNP component IST3

MacromoleculeName: U2 snRNP component IST3 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 17.121127 KDa
SequenceString:
MNKIQQINDK ELQSGILSPH QSWHNEYKDN AYIYIGNLNR ELTEGDILTV FSEYGVPVDV ILSRDENTGE SQGFAYLKYE DQRSTILAV DNLNGFKIGG RALKIDHTFY RPKRSLQKYY EAVKEELDRD IVSKNNAEKL ILAKKDQPN

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Macromolecule #22: Pre-mRNA-splicing factor CWC26

MacromoleculeName: Pre-mRNA-splicing factor CWC26 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 30.529141 KDa
SequenceString: MALHQYLSET YGPTKPKNKT KKKKKESKSD ANSDKTSLIV KERLSTLQQE QEKSGVASFS KFDKQKSKNI WKNLETNELS HAITHPSAS SITGNESKND LKEIRAQEPL VTVADKSKTR KTIYRDAQGH KIQEDSKIDD SSFSRSKYED EKAAEREQYL K NLNMGDVQ ...String:
MALHQYLSET YGPTKPKNKT KKKKKESKSD ANSDKTSLIV KERLSTLQQE QEKSGVASFS KFDKQKSKNI WKNLETNELS HAITHPSAS SITGNESKND LKEIRAQEPL VTVADKSKTR KTIYRDAQGH KIQEDSKIDD SSFSRSKYED EKAAEREQYL K NLNMGDVQ KLGINVDAHD KKKNQTASSL TIEDPAITFT HDKERTVKTS LLGRKLYDKP APENRFAIMP GSRWDGVHRS NG FEEKWFA KQNEINEKKV QSYTLQEDY

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Macromolecule #23: Pre-mRNA-splicing factor ATP-dependent RNA helicase-like protein PRP2

MacromoleculeName: Pre-mRNA-splicing factor ATP-dependent RNA helicase-like protein PRP2
type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA helicase
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 99.947492 KDa
SequenceString: MSSITSETGK RRVKRTYEVT RQNDNAVRIE PSSLGEEEDK EAKDKNSALQ LKRSRYDPNK VFSNTNQGPE KNNLKGEQLG SQKKSSKYD EKITSNNELT TKKGLLGDSE NETKYASSNS KFNVEVTHKI KNAKEIDKIN RQRMWEEQQL RNAMAGQSDH P DDITLEGS ...String:
MSSITSETGK RRVKRTYEVT RQNDNAVRIE PSSLGEEEDK EAKDKNSALQ LKRSRYDPNK VFSNTNQGPE KNNLKGEQLG SQKKSSKYD EKITSNNELT TKKGLLGDSE NETKYASSNS KFNVEVTHKI KNAKEIDKIN RQRMWEEQQL RNAMAGQSDH P DDITLEGS DKYDYVFDTD AMIDYTNEED DLLPEEKLQY EARLAQALET EEKRILTIQE ARKLLPVHQY KDELLQEIKK NQ VLIIMGE TGSGKTTQLP QYLVEDGFTD QGKLQIAITQ PRRVAATSVA ARVADEMNVV LGKEVGYQIR FEDKTTPNKT VLK YMTDGM LLREFLTDSK LSKYSCIMID EAHERTLATD ILIGLLKDIL PQRPTLKLLI SSATMNAKKF SEFFDNCPIF NVPG RRYPV DIHYTLQPEA NYIHAAITTI FQIHTTQSLP GDILVFLTGQ EEIERTKTKL EEIMSKLGSR TKQMIITPIY ANLPQ EQQL KIFQPTPENC RKVVLATNIA ETSLTIDGIR YVIDPGFVKE NSYVPSTGMT QLLTVPCSRA SVDQRAGRAG RVGPGK CFR IFTKWSYLHE LELMPKPEIT RTNLSNTVLL LLSLGVTDLI KFPLMDKPSI PTLRKSLENL YILGALNSKG TITRLGK MM CEFPCEPEFA KVLYTAATHE QCQGVLEECL TIVSMLHETP SLFIGQKRDA AASVLSEVES DHILYLEIFN QWRNSKFS R SWCQDHKIQF KTMLRVRNIR NQLFRCSEKV GLVEKNDQAR MKIGNIAGYI NARITRCFIS GFPMNIVQLG PTGYQTMGR SSGGLNVSVH PTSILFVNHK EKAQRPSKYV LYQQLMLTSK EFIRDCLVIP KEEWLIDMVP QIFKDLIDDK TNRGRR

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Macromolecule #24: Pre-mRNA-splicing factor CWC22

MacromoleculeName: Pre-mRNA-splicing factor CWC22 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 67.386062 KDa
SequenceString: MSTATIQDED IKFQRENWEM IRSHVSPIIS NLTMDNLQES HRDLFQVNIL IGRNIICKNV VDFTLNKQNG RLIPALSALI ALLNSDIPD IGETLAKELM LMFVQQFNRK DYVSCGNILQ CLSILFLYDV IHEIVILQIL LLLLEKNSLR LVIAVMKICG W KLALVSKK ...String:
MSTATIQDED IKFQRENWEM IRSHVSPIIS NLTMDNLQES HRDLFQVNIL IGRNIICKNV VDFTLNKQNG RLIPALSALI ALLNSDIPD IGETLAKELM LMFVQQFNRK DYVSCGNILQ CLSILFLYDV IHEIVILQIL LLLLEKNSLR LVIAVMKICG W KLALVSKK THDMIWEKLR YILQTQELSS TLRESLETLF EIRQKDYKSG SQGLFILDPT SYTVHTHSYI VSDEDEANKE LG NFEKCEN FNELTMAFDT LRQKLLINNT SDTNEGSNSQ LQIYDMTSTN DVEFKKKIYL VLKSSLSGDE AAHKLLKLKI ANN LKKSVV DIIIKSSLQE STFSKFYSIL SERMITFHRS WQTAYNETFE QNYTQDIEDY ETDQLRILGK FWGHLISYEF LPMD CLKII KLTEEESCPQ GRIFIKFLFQ ELVNELGLDE LQLRLNSSKL DGMFPLEGDA EHIRYSINFF TAIGLGLLTE DMRSR LTII QEVEDAEEEE KKLREEEELE KLRKKARESQ PTQGPKIHES RLFLQKDTRE NSRSRSPFTV ETRKRARSRT PPRGSR NHR NRSRTPPARR QRHR

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Macromolecule #25: Pre-mRNA-splicing factor CWC24

MacromoleculeName: Pre-mRNA-splicing factor CWC24 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 29.797941 KDa
SequenceString: MFRKRLVNKS SSDEKNQKKR QKINFSEEKL VASDEEKGSS DLMSLAKSGN SRTLQLSHEN EGKLQKKGED LDKYTLTVND DSTKEDLLN FERKELAEKA KKRRPSDDNE LVLNMSGKNK RLTKQINQPT NIRTTVLMDF QPDVCKDYKQ TGYCGYGDSC K FLHSRDDF ...String:
MFRKRLVNKS SSDEKNQKKR QKINFSEEKL VASDEEKGSS DLMSLAKSGN SRTLQLSHEN EGKLQKKGED LDKYTLTVND DSTKEDLLN FERKELAEKA KKRRPSDDNE LVLNMSGKNK RLTKQINQPT NIRTTVLMDF QPDVCKDYKQ TGYCGYGDSC K FLHSRDDF KTGWKLNQEW NADKEDSKAV TLDLEKIPFK CTLCKEDYKS PVVTNCGHYF CGSCFAKDMK KGTKCFICHK ET HGSAKVA SDLQKMLNKR KS

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Macromolecule #26: Peptidyl-prolyl isomerase CWC27

MacromoleculeName: Peptidyl-prolyl isomerase CWC27 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO / EC number: peptidylprolyl isomerase
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 35.08093 KDa
SequenceString: MSSNIEPQTT AKCILYTTKG NIAIELWAKE CPETCKRFLS MLSDGTFTNG EFKELKPTQW LMFNANSTGE YRTVAEEKNP RIRFNRDGL LGWDRRRNTW FITVLADSKH VLNDCNVFGK IVGKSIYIFR EILGGEIEAS SRDNDVKRFM YPAVLKDVEI T IPFFEDIF ...String:
MSSNIEPQTT AKCILYTTKG NIAIELWAKE CPETCKRFLS MLSDGTFTNG EFKELKPTQW LMFNANSTGE YRTVAEEKNP RIRFNRDGL LGWDRRRNTW FITVLADSKH VLNDCNVFGK IVGKSIYIFR EILGGEIEAS SRDNDVKRFM YPAVLKDVEI T IPFFEDIF GSKRRLEDNE KKEQEPAKKL VKSAKVKMVY EDEQEDDDGD VQKLKPRKRM ILPAWIKDDS RSEGIKLDAS LD QPQEALI REKTELHDNV DEATTKETES QENIKEEPMD KRERETLAML SKFQERIKNK NILK

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Macromolecule #27: Pre-mRNA-splicing factor CEF1

MacromoleculeName: Pre-mRNA-splicing factor CEF1 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 61.057602 KDa
SequenceString: MPPVPIYVKG GVWTNVEDQI LKAAVQKYGT HQWSKVASLL QKKTARQSEL RWNEYLNPKL NFTEFSKEED AQLLDLAREL PNQWRTIAD MMARPAQVCV ERYNRLLESE DSGGAALSTG VTDLKAGDIN PNAETQMARP DNGDLEDEEK EMLAEARARL L NTQGKKAT ...String:
MPPVPIYVKG GVWTNVEDQI LKAAVQKYGT HQWSKVASLL QKKTARQSEL RWNEYLNPKL NFTEFSKEED AQLLDLAREL PNQWRTIAD MMARPAQVCV ERYNRLLESE DSGGAALSTG VTDLKAGDIN PNAETQMARP DNGDLEDEEK EMLAEARARL L NTQGKKAT RKIRERMLEE SKRIAELQKR RELKQAGINV AIKKPKKKYG TDIDYNEDIV YEQAPMPGIY DTSTEDRQIK KK FEQFERK VNRKGL(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)ES RMQHITQGRT SMKIQFKTAM PPTEVLLESI QSKVESIEQL QRK LQHVQP LEQQNNEMCS TLCHHSLPAL IEGQRKYYAD YYAYRQEIRS LEGRRKRLQA MLNSS

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Macromolecule #28: U2 snRNP component HSH155

MacromoleculeName: U2 snRNP component HSH155 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 110.166672 KDa
SequenceString: MSHPIQFVNA NNSDKSHQLG GQYSIPQDLR ENLQKEAARI GENEKDVLQE KMETRTVQNR EDSYHKRRFD MKFEPDSDTQ TVTSSENTQ DAVVPRKRKS RWDVKGYEPP DESSTAVKEN SDSALVNVEG IHDLMFFKPS DHKYFADVIS KKPIDELNKD E KKERTLSM ...String:
MSHPIQFVNA NNSDKSHQLG GQYSIPQDLR ENLQKEAARI GENEKDVLQE KMETRTVQNR EDSYHKRRFD MKFEPDSDTQ TVTSSENTQ DAVVPRKRKS RWDVKGYEPP DESSTAVKEN SDSALVNVEG IHDLMFFKPS DHKYFADVIS KKPIDELNKD E KKERTLSM LLLKIKNGNT ASRRTSMRIL TDKAVTFGPE MIFNRLLPIL LDRSLEDQER HLMIKTIDRV LYQLGDLTKP YV HKILVVA APLLIDEDPM VRSTGQEIIT NLSTVAGLKT ILTVMRPDIE NEDEYVRNVT SRAAAVVAKA LGVNQLLPFI NAA CHSRKS WKARHTGIKI VQQIGILLGI GVLNHLTGLM SCIKDCLMDD HVPVRIVTAH TLSTLAENSY PYGIEVFNVV LEPL WKGIR SHRGKVLSSF LKAVGSMIPL MDPEYAGYYT TEAMRIIRRE FDSPDDEMKK TILLVLQKCS AVESITPKFL REEIA PEFF QKFWVRRVAL DRPLNKVVTY TTVTLAKKLG CSYTIDKLLT PLRDEAEPFR TMAVHAVTRT VNLLGTADLD ERLETR LID ALLIAFQEQT NSDSIIFKGF GAVTVSLDIR MKPFLAPIVS TILNHLKHKT PLVRQHAADL CAILIPVIKN CHEFEML NK LNIILYESLG EVYPEVLGSI INAMYCITSV MDLDKLQPPI NQILPTLTPI LRNKHRKVEV NTIKFVGLIG KLAPTYAP P KEWMRICFEL LELLKSTNKE IRRSANATFG FIAEAIGPHD VLVALLNNLK VQERQLRVCT AVAIGIVAKV CGPYNVLPV IMNEYTTPET NVQNGVLKAM SFMFEYIGNM SKDYIYFITP LLEDALTDRD LVHRQTASNV ITHLALNCSG TGHEDAFIHL MNLLIPNIF ETSPHAIMRI LEGLEALSQA LGPGLFMNYI WAGLFHPAKN VRKAFWRVYN NMYVMYQDAM VPFYPVTPDN N EEYIEELD LVL

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Macromolecule #29: Pre-mRNA-splicing factor CLF1

MacromoleculeName: Pre-mRNA-splicing factor CLF1 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288c (yeast)
Molecular weightTheoretical: 68.04457 KDa
SequenceString: MDTLEPTAVD THVSAEQILR DVYKKGQKAR GSTNIDILDL EELREYQRRK RTEYEGYLKR NRLDMGQWIR YAQFEIEQHD MRRARSIFE RALLVDSSFI PLWIRYIDAE LKVKCINHAR NLMNRAISTL PRVDKLWYKY LIVEESLNNV EIVRSLYTKW C SLEPGVNA ...String:
MDTLEPTAVD THVSAEQILR DVYKKGQKAR GSTNIDILDL EELREYQRRK RTEYEGYLKR NRLDMGQWIR YAQFEIEQHD MRRARSIFE RALLVDSSFI PLWIRYIDAE LKVKCINHAR NLMNRAISTL PRVDKLWYKY LIVEESLNNV EIVRSLYTKW C SLEPGVNA WNSFVDFEIR QKNWNGVREI YSKYVMAHPQ MQTWLKWVRF ENRHGNTEFT RSVYSLAIDT VANLQNLQIW SD MEVAKLV NSFAHWEAAQ QEYERSSALY QIAIEKWP(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)

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Macromolecule #30: Pre-mRNA-splicing factor CWC21

MacromoleculeName: Pre-mRNA-splicing factor CWC21 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 15.793596 KDa
SequenceString:
MSYNGIGLKS AKGSSTSGHV QRSLASNNRR RPQGSQQQRQ QRQNAIKKAS HDKASRPLAV QKQIETHMEK REIEVQVSEL RDRLEEEET LSEEQIDKKC EALRAKLTNE WQEQQRMSSL YTPRKARLTE EQHRHE

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Macromolecule #31: Pre-mRNA-splicing factor SYF1

MacromoleculeName: Pre-mRNA-splicing factor SYF1 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288c (yeast)
Molecular weightTheoretical: 78.12557 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)KTKMAYN TVIDLRLITP AMAENFALFL QNHYEVMESF QVY EKTIPL FPPEIQYELW IEYLEVATSH QLSSLSPEHI RFLFEKALKN LCSNGIDCKT IFIAYSVFEE RISGLISKSI EILR RGAVI GTVSVSTHLE SRLQLWRMCI SKAESTLGPS VTRELYQECI Q(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)

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Macromolecule #32: Protein HSH49

MacromoleculeName: Protein HSH49 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 24.534152 KDa
SequenceString: MNYSADSGNT VYVGNIDPRI TKEQLYELFI QINPVLRIKY PKDKVLQAYQ GYAFIEFYNQ GDAQYAIKIM NNTVRLYDRL IKVRQVTNS TGTTNLPSNI SKDMILPIAK LFIKNLADSI DSDQLVKIFN KFGKLIREPE IFYLSNGKLK CAYVYFEDFE K ADLAIKSL ...String:
MNYSADSGNT VYVGNIDPRI TKEQLYELFI QINPVLRIKY PKDKVLQAYQ GYAFIEFYNQ GDAQYAIKIM NNTVRLYDRL IKVRQVTNS TGTTNLPSNI SKDMILPIAK LFIKNLADSI DSDQLVKIFN KFGKLIREPE IFYLSNGKLK CAYVYFEDFE K ADLAIKSL NNQLVANNRI TVDYAFKENG KGNAKYGDDV DRLLNKEALK HNMLK

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Macromolecule #33: Pre-mRNA-splicing factor SYF2

MacromoleculeName: Pre-mRNA-splicing factor SYF2 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 24.850719 KDa
SequenceString: MDFYKLDEKL KELKRKRVDV SIKSRKLADR EIQEVSANRK PRVYSMEDVN DADESVGDTE SPEKEKAFHY TVQEYDAWER RHPQGKTGQ SQRGGISYDQ LAKLSYEKTL RNLATQTQNS SKQDSSADEE DNKNVPKKGR IGKVQKDTKT GKITIADDDK L VNKLAVSL ...String:
MDFYKLDEKL KELKRKRVDV SIKSRKLADR EIQEVSANRK PRVYSMEDVN DADESVGDTE SPEKEKAFHY TVQEYDAWER RHPQGKTGQ SQRGGISYDQ LAKLSYEKTL RNLATQTQNS SKQDSSADEE DNKNVPKKGR IGKVQKDTKT GKITIADDDK L VNKLAVSL QSESKKRYEA RKRQMQNAKT LYGVESFIND KNKQFNEKLS RESKGSE

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Macromolecule #34: Pre-mRNA-processing factor 19

MacromoleculeName: Pre-mRNA-processing factor 19 / type: protein_or_peptide / ID: 34 / Number of copies: 4 / Enantiomer: LEVO
EC number: Ligases; Forming carbon-nitrogen bonds; Acid-amino-acid ligases (peptide synthases)
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 56.629777 KDa
SequenceString: MLCAISGKVP RRPVLSPKSR TIFEKSLLEQ YVKDTGNDPI TNEPLSIEEI VEIVPSAQQA SLTESTNSAT LKANYSIPNL LTSLQNEWD AIMLENFKLR STLDSLTKKL STVMYERDAA KLVAAQLLME KNEDSKDLPK SSQQAVAITR EEFLQGLLQS S RDFVARGK ...String:
MLCAISGKVP RRPVLSPKSR TIFEKSLLEQ YVKDTGNDPI TNEPLSIEEI VEIVPSAQQA SLTESTNSAT LKANYSIPNL LTSLQNEWD AIMLENFKLR STLDSLTKKL STVMYERDAA KLVAAQLLME KNEDSKDLPK SSQQAVAITR EEFLQGLLQS S RDFVARGK LKAPKWPILK NLELLQAQNY SRNIKTFPYK ELNKSMYYDK WVCMCRCEDG ALHFTQLKDS KTITTITTPN PR TGGEHPA IISRGPCNRL LLLYPGNQIT ILDSKTNKVL REIEVDSANE IIYMYGHNEV NTEYFIWADN RGTIGFQSYE DDS QYIVHS AKSDVEYSSG VLHKDSLLLA LYSPDGILDV YNLSSPDQAS SRFPVDEEAK IKEVKFADNG YWMVVECDQT VVCF DLRKD VGTLAYPTYT IPEFKTGTVT YDIDDSGKNM IAYSNESNSL TIYKFDKKTK NWTKDEESAL CLQSDTADFT DMDVV CGDG GIAAILKTND SFNIVALTP

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Macromolecule #35: Pre-mRNA-splicing factor SNT309

MacromoleculeName: Pre-mRNA-splicing factor SNT309 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 20.741455 KDa
SequenceString:
MDGLSFVDKG KIPDGYKNEI DQLVKKEFAN IKREPVHPEI RGILAKRKGA DNSVSTLTNA LYTEYLKQRN NKKRRTPDFN DDDDTLFLE EYRRKYPRID TSRYIPNESS EVSLLGIVDS YLKHQEIVLD TLLPQTVSNQ WRINNDYIRQ TCTIVEEMNI Q QRKQINDL EIYRKRL

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Macromolecule #36: Pre-mRNA-processing factor 17

MacromoleculeName: Pre-mRNA-processing factor 17 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 52.128762 KDa
SequenceString: MGLVDGYDTS SDSDLNFDEG KSVHEKKNGN LHEDTSYEPS SNNIHKRKSH FTKSELKRRR KTRKGDGPWG SWSSSDDETS QASETQKED QDIFVHALAE DNLDSEQIEV EEVSHFYGKS EKDYQGRGYL YPPNDVDVDL REERISFRCY LPKKVIRNYP G HPEGTTAL ...String:
MGLVDGYDTS SDSDLNFDEG KSVHEKKNGN LHEDTSYEPS SNNIHKRKSH FTKSELKRRR KTRKGDGPWG SWSSSDDETS QASETQKED QDIFVHALAE DNLDSEQIEV EEVSHFYGKS EKDYQGRGYL YPPNDVDVDL REERISFRCY LPKKVIRNYP G HPEGTTAL KFLPKTGHLI LSGGNDHTIK IWDFYHDYEC LRDFQGHNKP IKALRFTEDC QSFLSSSFDR SVKIWDTETG KV KTRLHLN STPADVESRP TNPHEFIVGL SNSKILHYDD RVSENQGLVQ TYDHHLSSIL ALKYFPDGSK FISSSEDKTV RIW ENQINV PIKQISDTAQ HSMPFLNVHP SQNYFCAQSM DNRIYSFSLK PKYKRHPKKI FKGHSSAGYG ISLAFSGDGR YICS GDSKS RLFTWDWNTS RLLNNIKIPG NKPITQVDWH PQETSKVICS GAAGKIYVCD

+
Macromolecule #37: Small nuclear ribonucleoprotein-associated protein B

MacromoleculeName: Small nuclear ribonucleoprotein-associated protein B / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 22.42699 KDa
SequenceString: MSKIQVAHSS RLANLIDYKL RVLTQDGRVY IGQLMAFDKH MNLVLNECIE ERVPKTQLDK LRPRKDSKDG TTLNIKVEKR VLGLTILRG EQILSTVVED KPLLSKKERL VRDKKEKKQA QKQTKLRKEK EKKPGKIAKP NTANAKHTSS NSREIAQPSS S RYNGGNDN ...String:
MSKIQVAHSS RLANLIDYKL RVLTQDGRVY IGQLMAFDKH MNLVLNECIE ERVPKTQLDK LRPRKDSKDG TTLNIKVEKR VLGLTILRG EQILSTVVED KPLLSKKERL VRDKKEKKQA QKQTKLRKEK EKKPGKIAKP NTANAKHTSS NSREIAQPSS S RYNGGNDN IGANRSRFNN EAPPQTRKFQ PPPGFKRK

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Macromolecule #38: Small nuclear ribonucleoprotein E

MacromoleculeName: Small nuclear ribonucleoprotein E / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 10.385098 KDa
SequenceString:
MSNKVKTKAM VPPINCIFNF LQQQTPVTIW LFEQIGIRIK GKIVGFDEFM NVVIDEAVEI PVNSADGKED VEKGTPLGKI LLKGDNITL ITSAD

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Macromolecule #39: Small nuclear ribonucleoprotein F

MacromoleculeName: Small nuclear ribonucleoprotein F / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 9.669945 KDa
SequenceString:
MSESSDISAM QPVNPKPFLK GLVNHRVGVK LKFNSTEYRG TLVSTDNYFN LQLNEAEEFV AGVSHGTLGE IFIRCNNVLY IRELPN

+
Macromolecule #40: Small nuclear ribonucleoprotein G

MacromoleculeName: Small nuclear ribonucleoprotein G / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 8.490809 KDa
SequenceString:
MVSTPELKKY MDKKILLNIN GSRKVAGILR GYDIFLNVVL DDAMEINGED PANNHQLGLQ TVIRGNSIIS LEALDAI

+
Macromolecule #41: Small nuclear ribonucleoprotein Sm D3

MacromoleculeName: Small nuclear ribonucleoprotein Sm D3 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 11.240139 KDa
SequenceString:
MTMNGIPVKL LNEAQGHIVS LELTTGATYR GKLVESEDSM NVQLRDVIAT EPQGAVTHMD QIFVRGSQIK FIVVPDLLKN APLFKKNSS RPMPPIRGPK RR

+
Macromolecule #42: Small nuclear ribonucleoprotein Sm D1

MacromoleculeName: Small nuclear ribonucleoprotein Sm D1 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 16.296798 KDa
SequenceString:
MKLVNFLKKL RNEQVTIELK NGTTVWGTLQ SVSPQMNAIL TDVKLTLPQP RLNKLNSNGI AMASLYLTGG QQPTASDNIA SLQYINIRG NTIRQIILPD SLNLDSLLVD QKQLNSLRRS GQIANDPSKK RRRDFGAPAN KRPRRGL

+
Macromolecule #43: Small nuclear ribonucleoprotein Sm D2

MacromoleculeName: Small nuclear ribonucleoprotein Sm D2 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast) / Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 11.021953 KDa
SequenceString:
RAELEELEEF EFKHGPMSLI NDAMVTRTPV IISLRNNHKI IARVKAFDRH CNMVLENVKE LWTEKKGKNV INRERFISKL FLRGDSVIV VLKTP

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Macromolecule #4: Saccharomyces cerevisiae strain CDRDR_sf_H chromosome VII sequence

MacromoleculeName: Saccharomyces cerevisiae strain CDRDR_sf_H chromosome VII sequence
type: rna / ID: 4 / Number of copies: 1
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 68.643344 KDa
SequenceString: AAGCAGCUUU ACAGAUCAAU GGCGGAGGGA GGUCAACAUC AAGAACUGUG GGCCUUUUAU UGCCUAUAGA ACUUAUAACG AACAUGGUU CUUGCCUUUU ACCAGAACCA UCCGGGUGUU GUCUCCAUAG AAACAGGUAA AGCUGUCCGU UACUGUGGGC U UGCCAUAU ...String:
AAGCAGCUUU ACAGAUCAAU GGCGGAGGGA GGUCAACAUC AAGAACUGUG GGCCUUUUAU UGCCUAUAGA ACUUAUAACG AACAUGGUU CUUGCCUUUU ACCAGAACCA UCCGGGUGUU GUCUCCAUAG AAACAGGUAA AGCUGUCCGU UACUGUGGGC U UGCCAUAU UUUUUGGAAC UUUUCUGCCC UUUUUCUCAA UGAGUAAGGA GGGCGU

+
Macromolecule #5: Saccharomyces cerevisiae strain T.52_2H chromosome XII sequence

MacromoleculeName: Saccharomyces cerevisiae strain T.52_2H chromosome XII sequence
type: rna / ID: 5 / Number of copies: 1
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 35.883176 KDa
SequenceString:
GUUCGCGAAG UAACCCUUCG UGGACAUUUG GUCAAUUUGA AACAAUACAG AGAUGAUCAG CAGUUCCCCU GCAUAAGGAU GAACCGUUU UACAAAGAGA UUUAUUUCGU UUU

+
Macromolecule #11: U2 snRNA

MacromoleculeName: U2 snRNA / type: rna / ID: 11 / Number of copies: 1
Source (natural)Organism: Saccharomyces cerevisiae S288c (yeast)
Molecular weightTheoretical: 376.267406 KDa
SequenceString: ACGAAUCUCU UUGCCUUUUG GCUUAGAUCA AGUGUAGUAU CUGUUCUUUU CAGUGUAACA ACUGAAAUGA CCUCAAUGAG GCUCAUUAC CUUUUAAUUU GUUACAAUAC ACAUUUUUUG GCACCCAAAA UAAUAAAAUG GACGGGAAGA GACUUUUUAA G CAAGUUGU ...String:
ACGAAUCUCU UUGCCUUUUG GCUUAGAUCA AGUGUAGUAU CUGUUCUUUU CAGUGUAACA ACUGAAAUGA CCUCAAUGAG GCUCAUUAC CUUUUAAUUU GUUACAAUAC ACAUUUUUUG GCACCCAAAA UAAUAAAAUG GACGGGAAGA GACUUUUUAA G CAAGUUGU UUUCCGCUAA UGUCAGGUCU CACUACUUUU UGCUGCUAUU UUUCUUCGCU CAUGGUUUCU UCAUAAGGCG UU UUUAUGA UGGUUUUUCG AAAUUGGUUU UUGAGACGAC GGUUGCUCAA GGUUAUUGUU UUUGUUUUCU UCUGGUUGUU UUC UAUUUU CUUUUUUUUA GCUUUCUGUU UCUCCCUUAG UUUGGCUUUU UGCUUCAUAC UCUUCCCUGU CUUUCCGAGC CGUU UAUGU CCAACGCGGG AUUUGGUUUU UCUUUAUCGA UGGGAAGAAA UGGUGCUAUA GUAGGUUGGG AGAUAAUAUU UAUGG UAUG GGGUGCUAGU GCGGAUGGGG CGCUCUUAUU GUUGAUUUCU UCGCUCGUCU UCUUUUUCUG GUGGCGCUGC AAGAGG AAG UUUUUCGACU UUGUUAUGAU UUUUGGUUUG CAAGGAAAGG UGUCUUACGA UUCUUUUUUU GAUGUAAUAG GAUAAGC UU GCUUAUCCCC CAAGUAUCGG CCAAAGUUGU UGAUUUUCCU UUUGAAGUGU CCUCGGUUUG AGGGGGUGUA GGGUGGGG U UGGUCUACAA UAAGAGUGUU CCAUUGUUAA CGUGCUGGCG UCUUUUACUA UAUUUUUUUU CCCAGUUUAU UUUGUGCUU AUUUUCUCAU UGAGGAGAAG GAGCUCUUCU CGCAGGAUAU AAAUGGAGGU UUGCUAAAGG GGAGGAGAUG UGUUUGUGAG AAUACUGCU GAGAGAGUUC UGGAAGAGAA AAAAAGGAGG CAAUGGAAGG CGUUUGCUGG GAAAAGAGAA GAGCCAUGAC U GCAUCUGU UGUUUCAAGG CCAGUUUUAU UAACCGCCUA UGUCAUAGAG GCGUUUUUUU UGGAGGGAUU UGAAGAAUGC CG GCGGCAU CAAGAAACGG ACUUGAUGGU UGACGCCUGU UUUUAAAGUU AGAGACGUCG CGACCCUCGC ACUUGUGGAG UCG UUCUUG ACUUUUACUU UGGUCGCUUG AUGUUUCUCU CGUCUUCCCG UUCGCUCUU

+
Macromolecule #12: Pre-mRNA

MacromoleculeName: Pre-mRNA / type: rna / ID: 12 / Number of copies: 1
Source (natural)Organism: Saccharomyces cerevisiae S288c (yeast)
Molecular weightTheoretical: 7.864549 KDa
SequenceString:
AAUUUUUAAG GUAUGUAUUU UUUUU

+
Macromolecule #13: Pre-mRNA

MacromoleculeName: Pre-mRNA / type: rna / ID: 13 / Number of copies: 1
Source (natural)Organism: Saccharomyces cerevisiae S288c (yeast)
Molecular weightTheoretical: 18.915268 KDa
SequenceString:
AAAAAAAAAA AAAANNNAAA AAANNAAAAC UAGAUACUAA CACAUUUAAU UUUUUUUUGU U

+
Macromolecule #44: GUANOSINE-5'-TRIPHOSPHATE

MacromoleculeName: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 44 / Number of copies: 1 / Formula: GTP
Molecular weightTheoretical: 523.18 Da
Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

+
Macromolecule #45: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 45 / Number of copies: 5 / Formula: MG
Molecular weightTheoretical: 24.305 Da

+
Macromolecule #46: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 46 / Number of copies: 13 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

+
Macromolecule #47: ADENOSINE-5'-DIPHOSPHATE

MacromoleculeName: ADENOSINE-5'-DIPHOSPHATE / type: ligand / ID: 47 / Number of copies: 1 / Formula: ADP
Molecular weightTheoretical: 427.201 Da
Chemical component information

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 8
Details: CEB buffer (10 mM Tris-HCl, pH 8.0, 75 mM NaCl, 1 mM Mg(OAc)2, 1 mM imidazole, 0.01% NP40, 1 mM TCEP, 0.5 mM EGTA)
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Digitization - Scanner: OTHER / Average electron dose: 4.7 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.5 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 77312
Initial angle assignmentType: ANGULAR RECONSTITUTION
Final angle assignmentType: ANGULAR RECONSTITUTION

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Atomic model buiding 1

RefinementProtocol: AB INITIO MODEL
Output model

PDB-5gm6:
Cryo-EM structure of the activated spliceosome (Bact complex) at 3.5 angstrom resolution

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