Natural Sciences and Engineering Research Council (NSERC, Canada)
69462
カナダ
引用
ジャーナル: Nat Commun / 年: 2017 タイトル: Subnanometre-resolution structure of the doublet microtubule reveals new classes of microtubule-associated proteins. 著者: Muneyoshi Ichikawa / Dinan Liu / Panagiotis L Kastritis / Kaustuv Basu / Tzu Chin Hsu / Shunkai Yang / Khanh Huy Bui / 要旨: Cilia are ubiquitous, hair-like appendages found in eukaryotic cells that carry out functions of cell motility and sensory reception. Cilia contain an intriguing cytoskeletal structure, termed the ...Cilia are ubiquitous, hair-like appendages found in eukaryotic cells that carry out functions of cell motility and sensory reception. Cilia contain an intriguing cytoskeletal structure, termed the axoneme that consists of nine doublet microtubules radially interlinked and longitudinally organized in multiple specific repeat units. Little is known, however, about how the axoneme allows cilia to be both actively bendable and sturdy or how it is assembled. To answer these questions, we used cryo-electron microscopy to structurally analyse several of the repeating units of the doublet at sub-nanometre resolution. This structural detail enables us to unambiguously assign α- and β-tubulins in the doublet microtubule lattice. Our study demonstrates the existence of an inner sheath composed of different kinds of microtubule inner proteins inside the doublet that likely stabilizes the structure and facilitates the specific building of the B-tubule.
想定した対称性 - 点群: C1 (非対称) / 解像度のタイプ: BY AUTHOR / 解像度: 4.6 Å / 解像度の算出法: FSC 0.143 CUT-OFF / ソフトウェア - 名称: AV3 詳細: 23 boxes of the protofilament are boxed out from the doublet map (EMD-8528), aligned and averaged using subtomogram averaging software. 使用した粒子像数: 23
初期 角度割当
タイプ: OTHER / ソフトウェア - 名称: MATLAB / 詳細: Geometry calculated from the microtubule doublet
最終 角度割当
タイプ: OTHER / ソフトウェア - 名称: AV3 / 詳細: Alignment using Tom AV3 software
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原子モデル構築 1
精密化
空間: REAL / プロトコル: BACKBONE TRACE / 当てはまり具合の基準: minimization global
得られたモデル
PDB-5ucy: Cryo-EM map of protofilament of microtubule doublet