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TitleSubnanometre-resolution structure of the doublet microtubule reveals new classes of microtubule-associated proteins.
Journal, issue, pagesNat Commun, Vol. 8, Page 15035, Year 2017
Publish dateMay 2, 2017
AuthorsMuneyoshi Ichikawa / Dinan Liu / Panagiotis L Kastritis / Kaustuv Basu / Tzu Chin Hsu / Shunkai Yang / Khanh Huy Bui /
PubMed AbstractCilia are ubiquitous, hair-like appendages found in eukaryotic cells that carry out functions of cell motility and sensory reception. Cilia contain an intriguing cytoskeletal structure, termed the ...Cilia are ubiquitous, hair-like appendages found in eukaryotic cells that carry out functions of cell motility and sensory reception. Cilia contain an intriguing cytoskeletal structure, termed the axoneme that consists of nine doublet microtubules radially interlinked and longitudinally organized in multiple specific repeat units. Little is known, however, about how the axoneme allows cilia to be both actively bendable and sturdy or how it is assembled. To answer these questions, we used cryo-electron microscopy to structurally analyse several of the repeating units of the doublet at sub-nanometre resolution. This structural detail enables us to unambiguously assign α- and β-tubulins in the doublet microtubule lattice. Our study demonstrates the existence of an inner sheath composed of different kinds of microtubule inner proteins inside the doublet that likely stabilizes the structure and facilitates the specific building of the B-tubule.
External linksNat Commun / PubMed:28462916 / PubMed Central
MethodsEM (single particle)
Resolution4.6 - 8.6 Å
Structure data

8528

5ubq

EMDB-8528: Cryo-EM structure of 8nm repeat tubulin lattice of the ciliary microtubule doublet
PDB-5ubq: Cryo-EM structure of ciliary microtubule doublet
Method: EM (single particle) / Resolution: 5.7 Å

EMDB-8532:
Cryo-EM structure of 16nm repeat ciliary microtubule doublet
Method: EM (single particle) / Resolution: 6.2 Å

EMDB-8537:
Cryo-EM structure of 48nm repeat ciliary microtubule doublet
Method: EM (single particle) / Resolution: 8.6 Å

8539

5ucy

EMDB-8539, PDB-5ucy:
Cryo-EM map of protofilament of microtubule doublet
Method: EM (single particle) / Resolution: 4.6 Å

Chemicals

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate

ChemComp-MG:
Unknown entry

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM / Guanosine diphosphate

Source
  • tetrahymena thermophila (eukaryote)
KeywordsSTRUCTURAL PROTEIN / cilia / doublet / axoneme / tubulin / protofilament

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