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- PDB-7kzt: Structure of the human fanconi anaemia Core-UBE2T-ID-DNA complex ... -

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Basic information

Entry
Database: PDB / ID: 7kzt
TitleStructure of the human fanconi anaemia Core-UBE2T-ID-DNA complex in intermediate state
Components
  • (DNA (22-MER)) x 2
  • (Fanconi anemia core complex-associated protein ...) x 2
  • (Fanconi anemia group ...) x 7
  • E3 ubiquitin-protein ligase FANCL
  • Fanconi anemia, complementation group I
  • Ubiquitin-conjugating enzyme E2 T
KeywordsLIGASE/DNA / Complex / LIGASE / LIGASE-DNA complex
Function / homology
Function and homology information


regulation of germ cell proliferation / regulation of CD40 signaling pathway / Fanconi anaemia nuclear complex / protein K29-linked ubiquitination / protein K27-linked ubiquitination / regulation of regulatory T cell differentiation / double-strand break repair involved in meiotic recombination / male meiotic nuclear division / homologous chromosome pairing at meiosis / gamete generation ...regulation of germ cell proliferation / regulation of CD40 signaling pathway / Fanconi anaemia nuclear complex / protein K29-linked ubiquitination / protein K27-linked ubiquitination / regulation of regulatory T cell differentiation / double-strand break repair involved in meiotic recombination / male meiotic nuclear division / homologous chromosome pairing at meiosis / gamete generation / replication-born double-strand break repair via sister chromatid exchange / neuronal stem cell population maintenance / protein K6-linked ubiquitination / protein K11-linked ubiquitination / brain morphogenesis / DNA repair complex / mitotic intra-S DNA damage checkpoint signaling / myeloid cell homeostasis / E2 ubiquitin-conjugating enzyme / female gonad development / germ cell development / protein monoubiquitination / ubiquitin conjugating enzyme activity / protein K63-linked ubiquitination / positive regulation of double-strand break repair via homologous recombination / spermatid development / protein autoubiquitination / protein K48-linked ubiquitination / negative regulation of double-strand break repair via homologous recombination / interstrand cross-link repair / DNA polymerase binding / ovarian follicle development / condensed chromosome / removal of superoxide radicals / Synthesis of active ubiquitin: roles of E1 and E2 enzymes / mitochondrion organization / Fanconi Anemia Pathway / nucleotide-excision repair / response to gamma radiation / TP53 Regulates Transcription of DNA Repair Genes / RING-type E3 ubiquitin transferase / response to radiation / PKR-mediated signaling / protein polyubiquitination / ubiquitin-protein transferase activity / male gonad development / ubiquitin protein ligase activity / nuclear envelope / chromosome / regulation of cell population proliferation / cellular response to oxidative stress / regulation of inflammatory response / protein-containing complex assembly / damaged DNA binding / nuclear body / intracellular membrane-bounded organelle / DNA repair / centrosome / ubiquitin protein ligase binding / DNA damage response / chromatin binding / chromatin / nucleolus / mitochondrion / DNA binding / nucleoplasm / ATP binding / nucleus / metal ion binding / plasma membrane / cytoplasm / cytosol
Similarity search - Function
Fanconi anaemia group C protein / Fanconi anemia-associated protein of 100kDa / Fanconi anemia group B protein / Fanconi anemia group G protein / Fanconi anaemia group C protein / Fanconi anemia-associated / Fanconi Anaemia group E protein, C-terminal / Fanconi anemia group F protein / FANCF, C-terminal domain superfamily / Fanconi anemia group E protein ...Fanconi anaemia group C protein / Fanconi anemia-associated protein of 100kDa / Fanconi anemia group B protein / Fanconi anemia group G protein / Fanconi anaemia group C protein / Fanconi anemia-associated / Fanconi Anaemia group E protein, C-terminal / Fanconi anemia group F protein / FANCF, C-terminal domain superfamily / Fanconi anemia group E protein / Fanconi anemia group F protein (FANCF) / Fanconi Anaemia group E protein FANCE / Fanconi anaemia group A protein / Fanconi anaemia group A protein, N-terminal domain / Fanconi anaemia group A protein / Fanconi anaemia group A protein N terminus / FANCL, UBC-like domain 2 / FANCL, UBC-like domain 3 / Fanconi anemia complex, subunit FancL, WD-repeat containing domain / E3 ubiquitin-protein ligase FANCL / FANCL C-terminal domain / FANCL, UBC-like domain 3 superfamily / FANCL UBC-like domain 1 / FANCL C-terminal domain / FANCL UBC-like domain 2 / FANCL UBC-like domain 3 / FANCL C-terminal domain / Fanconi anemia group I protein / FANCI solenoid 1 cap / FANCI solenoid 1 domain / FANCI helical domain 1 / FANCI helical domain 2 / FANCI solenoid 3 domain / FANCI solenoid 4 domain / FANCI solenoid 2 domain / FANCI solenoid 1 cap / FANCI solenoid 1 / FANCI solenoid 2 / FANCI solenoid 3 / FANCI solenoid 4 / FANCI helical domain 1 / FANCI helical domain 2 / Fanconi anaemia protein FANCD2 / Fanconi anaemia protein FancD2 nuclease / Ubiquitin-conjugating enzyme, active site / Ubiquitin-conjugating (UBC) active site signature. / Ubiquitin-conjugating enzyme E2, catalytic domain homologues / Ubiquitin-conjugating enzyme E2 / Ubiquitin-conjugating enzyme / Ubiquitin-conjugating (UBC) core domain profile. / Ubiquitin-conjugating enzyme/RWD-like / Tetratricopeptide repeats / Tetratricopeptide repeat / Tetratricopeptide-like helical domain superfamily / Zinc finger, RING/FYVE/PHD-type
Similarity search - Domain/homology
DNA / DNA (> 10) / Fanconi anemia, complementation group I / Fanconi anemia group G protein / Fanconi anemia group A protein / Fanconi anemia group C protein / Fanconi anemia core complex-associated protein 100 / Fanconi anemia group B protein / Fanconi anemia group D2 protein / Fanconi anemia group E protein ...DNA / DNA (> 10) / Fanconi anemia, complementation group I / Fanconi anemia group G protein / Fanconi anemia group A protein / Fanconi anemia group C protein / Fanconi anemia core complex-associated protein 100 / Fanconi anemia group B protein / Fanconi anemia group D2 protein / Fanconi anemia group E protein / Ubiquitin-conjugating enzyme E2 T / Fanconi anemia group F protein / E3 ubiquitin-protein ligase FANCL
Similarity search - Component
Biological speciesHomo sapiens (human)
synthetic construct (others)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 4.2 Å
AuthorsWang, S.L. / Pavletich, N.P.
Funding support United States, 1items
OrganizationGrant numberCountry
Howard Hughes Medical Institute (HHMI) United States
CitationJournal: Nat Struct Mol Biol / Year: 2021
Title: Structure of the FA core ubiquitin ligase closing the ID clamp on DNA.
Authors: Shengliu Wang / Renjing Wang / Christopher Peralta / Ayat Yaseen / Nikola P Pavletich /
Abstract: The Fanconi anemia (FA) pathway is essential for the repair of DNA interstrand crosslinks. Central to the pathway is the FA core complex, a ubiquitin ligase of nine subunits that monoubiquitinates ...The Fanconi anemia (FA) pathway is essential for the repair of DNA interstrand crosslinks. Central to the pathway is the FA core complex, a ubiquitin ligase of nine subunits that monoubiquitinates the FANCI-FANCD2 (ID) DNA clamp. The 3.1 Å structure of the 1.1-MDa human FA core complex, described here, reveals an asymmetric assembly with two copies of all but the FANCC, FANCE and FANCF subunits. The asymmetry is crucial, as it prevents the binding of a second FANCC-FANCE-FANCF subcomplex that inhibits the recruitment of the UBE2T ubiquitin conjugating enzyme, and instead creates an ID binding site. A single active site then ubiquitinates FANCD2 and FANCI sequentially. We also present the 4.2-Å structures of the human core-UBE2T-ID-DNA complex in three conformations captured during monoubiquitination. They reveal the core-UBE2T complex remodeling the ID-DNA complex, closing the clamp on the DNA before ubiquitination. Monoubiquitination then prevents clamp opening after release from the core.
History
DepositionDec 10, 2020Deposition site: RCSB / Processing site: RCSB
Revision 1.0Mar 10, 2021Provider: repository / Type: Initial release
Revision 1.1Mar 24, 2021Group: Database references / Category: citation / citation_author
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_PubMed / _citation.title / _citation_author.identifier_ORCID / _citation_author.name

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Structure visualization

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Assembly

Deposited unit
A: Fanconi anemia group A protein
B: Fanconi anemia group B protein
C: Fanconi anemia group C protein
E: Fanconi anemia group E protein
F: Fanconi anemia group F protein
G: Fanconi anemia group G protein
H: Fanconi anemia group G protein
L: E3 ubiquitin-protein ligase FANCL
O: Fanconi anemia group B protein
P: Fanconi anemia core complex-associated protein 100
Q: Fanconi anemia core complex-associated protein 100
S: Fanconi anemia group A protein
W: Fanconi anemia core complex-associated protein 20
M: E3 ubiquitin-protein ligase FANCL
X: Ubiquitin-conjugating enzyme E2 T
U: Fanconi anemia, complementation group I
V: Fanconi anemia group D2 protein
Y: DNA (22-MER)
Z: DNA (22-MER)
hetero molecules


Theoretical massNumber of molelcules
Total (without water)1,506,42724
Polymers1,506,10019
Non-polymers3275
Water00
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: microscopy
TypeNameSymmetry operationNumber
identity operation1_5551

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Components

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Fanconi anemia group ... , 7 types, 10 molecules ASBOCEFGHV

#1: Protein Fanconi anemia group A protein / Protein FACA


Mass: 165513.016 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: FANCA, FAA, FACA, FANCH / Production host: Homo sapiens (human) / References: UniProt: O15360
#2: Protein Fanconi anemia group B protein / Protein FACB / Fanconi anemia-associated polypeptide of 95 kDa / FAAP95


Mass: 100640.172 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: FANCB / Production host: Homo sapiens (human) / References: UniProt: Q8NB91
#3: Protein Fanconi anemia group C protein / Protein FACC


Mass: 66290.359 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: FANCC, FAC, FACC / Production host: Homo sapiens (human) / References: UniProt: Q00597
#4: Protein Fanconi anemia group E protein / Protein FACE


Mass: 61026.086 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: FANCE, FACE / Production host: Homo sapiens (human) / References: UniProt: Q9HB96
#5: Protein Fanconi anemia group F protein / Protein FACF


Mass: 45108.297 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: FANCF / Production host: Homo sapiens (human) / References: UniProt: Q9NPI8
#6: Protein Fanconi anemia group G protein / Protein FACG / DNA repair protein XRCC9


Mass: 70873.727 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: FANCG, XRCC9 / Production host: Homo sapiens (human) / References: UniProt: O15287
#12: Protein Fanconi anemia group D2 protein / Protein FACD2


Mass: 164325.516 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: FANCD2, FACD / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: Q9BXW9

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Protein , 3 types, 4 molecules LMXU

#7: Protein E3 ubiquitin-protein ligase FANCL / Fanconi anemia group L protein / Fanconi anemia-associated polypeptide of 43 kDa / FAAP43 / RING- ...Fanconi anemia group L protein / Fanconi anemia-associated polypeptide of 43 kDa / FAAP43 / RING-type E3 ubiquitin transferase FANCL


Mass: 45203.953 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: FANCL, PHF9 / Production host: Homo sapiens (human)
References: UniProt: Q9NW38, RING-type E3 ubiquitin transferase
#10: Protein Ubiquitin-conjugating enzyme E2 T / Cell proliferation-inducing gene 50 protein / E2 ubiquitin-conjugating enzyme T / Ubiquitin carrier ...Cell proliferation-inducing gene 50 protein / E2 ubiquitin-conjugating enzyme T / Ubiquitin carrier protein T / Ubiquitin-protein ligase T


Mass: 22553.873 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: UBE2T, HSPC150, PIG50 / Production host: Escherichia coli (E. coli)
References: UniProt: Q9NPD8, E2 ubiquitin-conjugating enzyme
#11: Protein Fanconi anemia, complementation group I


Mass: 149512.078 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: FANCI / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: B7ZMF2

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Fanconi anemia core complex-associated protein ... , 2 types, 3 molecules PQW

#8: Protein Fanconi anemia core complex-associated protein 100 / Fanconi anemia-associated protein of 100 kDa


Mass: 96513.898 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: FAAP100, C17orf70 / Production host: Homo sapiens (human) / References: UniProt: Q0VG06
#9: Protein/peptide Fanconi anemia core complex-associated protein 20


Mass: 4041.667 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human)

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DNA chain , 2 types, 2 molecules YZ

#13: DNA chain DNA (22-MER)


Mass: 18036.518 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#14: DNA chain DNA (22-MER)


Mass: 17716.326 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)

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Non-polymers , 1 types, 5 molecules

#15: Chemical
ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: Zn

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Details

Has ligand of interestN
Sequence detailsThe complete sequence of FAAP20 is MEAARRPRLGLSRRRPPPAGGPSGGRPWFLLGGDERERLWAELLRTVSPELILDHEVPSL ...The complete sequence of FAAP20 is MEAARRPRLGLSRRRPPPAGGPSGGRPWFLLGGDERERLWAELLRTVSPELILDHEVPSL PAFPGQEPRCGPEPTEVFTVGPKTFSWTPFPPDLWGPGRSYRLLHGAGGHLESPARSLPQ RPAPDPCRAPRVEQQPSVEGAAALRSCPMCQKEFAPRLTQLDVDSHLAQCLAESTEDVTW

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: Human Fanconi Anaemia Core-UBE2T-ID-DNA complex / Type: COMPLEX / Entity ID: #1-#14 / Source: RECOMBINANT
Molecular weightValue: 1.4 MDa / Experimental value: NO
Source (natural)Organism: Homo sapiens (human)
Source (recombinant)Organism: Homo sapiens (human)
Buffer solutionpH: 8
Buffer component
IDConc.NameFormulaBuffer-ID
120 mMtrisC4H11NO31
2100 mMsodium chlorideNaCl1
35 mMmagnesium chlorideMgCl21
45 mMAdenosine triphosphateC10H16N5O13P31
SpecimenConc.: 1 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD
Image recordingElectron dose: 36.5 e/Å2 / Detector mode: SUPER-RESOLUTION / Film or detector model: GATAN K3 (6k x 4k)

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Processing

SoftwareName: REFMAC / Version: 5.8.0267 / Classification: refinement
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstructionResolution: 4.2 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 69111 / Symmetry type: POINT
RefinementResolution: 4.2→20 Å / Cor.coef. Fo:Fc: 0.86 / SU B: 96.751 / SU ML: 0.504 / ESU R: 0.845
Stereochemistry target values: MAXIMUM LIKELIHOOD WITH PHASES
Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
RfactorNum. reflection% reflection
Rwork0.33724 --
obs0.33724 597325 99.08 %
Solvent computationIon probe radii: 0.9 Å / Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: MASK
Displacement parametersBiso mean: 115.132 Å2
Baniso -1Baniso -2Baniso -3
1-5.5 Å2-2.04 Å2-1.86 Å2
2---1.9 Å2-4.91 Å2
3----3.6 Å2
Refinement stepCycle: 1 / Total: 87514
Refine LS restraints
Refine-IDTypeDev idealDev ideal targetNumber
ELECTRON MICROSCOPYr_bond_refined_d0.0090.01389357
ELECTRON MICROSCOPYr_bond_other_d0.0340.01786475
ELECTRON MICROSCOPYr_angle_refined_deg1.9851.626121239
ELECTRON MICROSCOPYr_angle_other_deg2.4171.58199218
ELECTRON MICROSCOPYr_dihedral_angle_1_deg10.307510892
ELECTRON MICROSCOPYr_dihedral_angle_2_deg35.53922.0614353
ELECTRON MICROSCOPYr_dihedral_angle_3_deg20.8131515706
ELECTRON MICROSCOPYr_dihedral_angle_4_deg19.62615582
ELECTRON MICROSCOPYr_chiral_restr0.0930.211610
ELECTRON MICROSCOPYr_gen_planes_refined0.0080.0298542
ELECTRON MICROSCOPYr_gen_planes_other0.0090.0220017
ELECTRON MICROSCOPYr_nbd_refined
ELECTRON MICROSCOPYr_nbd_other
ELECTRON MICROSCOPYr_nbtor_refined
ELECTRON MICROSCOPYr_nbtor_other
ELECTRON MICROSCOPYr_xyhbond_nbd_refined
ELECTRON MICROSCOPYr_xyhbond_nbd_other
ELECTRON MICROSCOPYr_metal_ion_refined
ELECTRON MICROSCOPYr_metal_ion_other
ELECTRON MICROSCOPYr_symmetry_vdw_refined
ELECTRON MICROSCOPYr_symmetry_vdw_other
ELECTRON MICROSCOPYr_symmetry_hbond_refined
ELECTRON MICROSCOPYr_symmetry_hbond_other
ELECTRON MICROSCOPYr_symmetry_metal_ion_refined
ELECTRON MICROSCOPYr_symmetry_metal_ion_other
ELECTRON MICROSCOPYr_mcbond_it3.95411.7343895
ELECTRON MICROSCOPYr_mcbond_other3.95411.7343894
ELECTRON MICROSCOPYr_mcangle_it7.07354678
ELECTRON MICROSCOPYr_mcangle_other7.07354679
ELECTRON MICROSCOPYr_scbond_it2.97312.0245462
ELECTRON MICROSCOPYr_scbond_other2.97312.0245461
ELECTRON MICROSCOPYr_scangle_it
ELECTRON MICROSCOPYr_scangle_other5.87666561
ELECTRON MICROSCOPYr_long_range_B_refined12.974100125
ELECTRON MICROSCOPYr_long_range_B_other12.974100126
ELECTRON MICROSCOPYr_rigid_bond_restr
ELECTRON MICROSCOPYr_sphericity_free
ELECTRON MICROSCOPYr_sphericity_bonded
LS refinement shellResolution: 4.2→4.304 Å / Total num. of bins used: 20
RfactorNum. reflection% reflection
Rfree0 0 -
Rwork0.443 42625 -
obs--100 %
Refinement TLS params.

Method: refined / Refine-ID: ELECTRON MICROSCOPY

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.62361.43610.06714.68171.19311.03980.2566-0.24870.1930.4759-0.0293-0.01140.2675-0.0549-0.22730.62750.2250.18581.51280.56831.177498.6625195.429863.9993
25.7363-2.3979-1.65866.21232.59492.5554-0.1281-0.36670.2574-0.2398-0.21630.6572-0.0121-0.24970.34440.49330.11410.19681.42780.38621.364871.8309179.716447.8828
32.9406-1.39340.51792.8597-0.58712.59560.01560.0446-0.312-0.3645-0.28320.9860.0271-0.470.26770.43390.2325-0.01751.49430.31931.819451.3683182.67938.9262
44.3499-1.93871.48755.28982.85593.38060.1089-0.0197-0.26060.27120.02940.09010.50570.1628-0.13830.7490.31010.19241.26260.7131.276191.9297160.761647.059
51.9556-0.3464-0.37295.5202-0.76052.7950.1542-0.28490.3937-0.25510.1223-0.1761-0.61280.2967-0.27650.61490.08120.37161.18770.37321.5376109.2229215.076253.2588
66.5711-5.57-0.59885.54220.35641.54580.37330.03030.5803-0.5301-0.2897-0.340.12950.098-0.08360.50330.3410.30581.31060.48291.4077138.4722188.418642.0065
73.17332.09061.67387.98681.37759.169-0.4682-0.20250.1740.43-0.0787-0.572-0.4571-0.2930.54690.77320.6548-0.02841.00230.47031.3664110.6105144.016167.8007
88.298-5.70622.38178.283-3.06545.46250.4874-0.1397-0.1008-0.19020.260.87370.6732-0.2379-0.74740.73160.41590.21111.25930.51011.198584.4508135.826838.316
98.3799-1.54142.00487.26940.34232.4690.05610.11870.1653-1.6117-0.31810.3680.15560.24850.2621.20910.4774-0.16171.20620.2130.995674.3552164.408820.0463
103.43021.49142.96091.28091.17092.6974-0.02241.1528-1.5237-1.20060.8319-1.3605-0.02531.0043-0.80942.95740.11340.93782.6282-0.01042.7673105.6216170.741227.064
117.8706-0.49183.90054.4693-0.10056.3136-0.1813-0.0812-0.21660.24620.073-0.0450.0809-0.59050.10830.87740.18470.6721.2850.34841.081877.660690.3565158.2985
1211.2981.78384.10667.02057.92449.35810.22452.02710.0869-0.0853-0.44030.22430.0148-0.03950.21581.10250.2270.59761.2960.3411.3177114.303887.2811179.765
132.15313.0133-2.34856.4454-0.43246.38140.0484-0.4171-0.3245-0.1619-0.1895-0.2171-0.43230.75190.14110.23550.44190.16051.43580.77481.6764116.219895.8518213.0602
143.8582-0.92040.31478.50072.48250.866-0.8898-0.77790.76942.30741.3791-0.79250.48770.1847-0.48931.11670.35590.29762.29890.93632.829395.451191.194224.5139
152.35330.62710.24692.6671-0.56320.43080.3883-0.5914-0.6117-0.0162-0.2635-0.54880.4338-0.0505-0.12480.90310.2115-0.01031.73061.14871.1796175.3985109.982563.7667
162.0144-1.01890.09376.38591.30151.06880.0060.09110.28770.2813-0.2107-0.4203-0.0058-0.01520.20480.69090.26150.30571.29360.60121.2107166.9042156.292447.013
172.66751.15940.52620.9527-0.18610.49520.15390.8791-0.388-0.26840.17180.22180.30190.4115-0.32570.85830.7218-0.21292.11540.41151.6858219.0585127.575569.2352
185.44920.48920.55961.06910.8440.71580.01540.2627-0.2727-0.16450.2978-0.3712-0.2310.2885-0.31320.73780.49750.09571.79160.87871.4542257.3392131.51497.1592
197.115-2.83485.0431.635-1.10336.8291-0.2314-0.39250.2839-0.1850.13160.1016-1.1924-0.23120.09980.9880.16460.16371.48260.83281.2833259.6463153.2756155.244
200.7933-0.07820.24452.3435-1.77794.7637-0.23240.2582-0.0182-0.2977-0.0391-0.1501-0.0544-0.07950.27150.68820.43890.26571.50130.65651.2047245.0262125.9674138.4456
213.22192.1207-0.30133.06310.14384.44480.3713-0.3739-0.2660.3926-0.0948-0.40970.11610.1834-0.27650.64390.40770.23611.67621.08311.2082267.6169130.2037179.6909
223.47351.72920.19184.01520.11550.1576-0.2385-0.1579-0.36730.01040.1672-0.5517-0.37090.11020.07141.0233-0.01870.20782.07760.89871.2231285.7628153.8999175.3519
233.97293.1331-0.47643.7453-0.49531.214-0.0925-0.20830.24240.6716-0.1305-0.44110.01030.28540.2230.67970.5024-0.02291.45110.93651.6639143.803181.5047139.6897
247.17761.828-2.42774.452-1.33052.68670.1388-0.50230.0372-0.6291-0.5270.8638-0.0441-0.30320.38820.22090.49420.08472.15540.51111.3196183.8463100.5815163.4719
250.41321.00720.29434.76230.96312.66840.2675-0.0703-0.2957-0.15880.0643-0.5586-0.54120.2179-0.33180.69680.1223-0.1411.11930.50822.0237155.2252126.5002125.7566
261.05321.111-0.01534.74482.0044.54990.40840.0272-0.2556-0.1251-0.19120.05060.1906-0.4623-0.21721.24690.1296-0.10441.170.26831.3726153.6736152.4518151.1872
270.9367-1.1881-2.1151.55582.7514.92550.114-0.12810.10390.08670.099-0.00110.26990.2767-0.2131.89520.15820.13391.47070.31191.2589156.3717165.3838177.5412
283.066-0.556-0.15614.8913-0.43270.221-0.2949-0.4570.5898-0.2363-0.03760.4398-0.00560.29270.33250.9250.48230.00541.4050.53951.2355228.1281148.5954176.2722
293.8707-0.2193-0.18424.19421.32121.00990.1378-0.2418-0.39880.188-0.3005-0.34920.1194-0.16760.16270.46120.50230.28951.57320.7941.344242.5823103.0116173.8531
306.49092.7494-1.37312.9245-1.66231.40750.01570.2064-0.5213-0.06430.3685-0.05140.2314-0.3357-0.38410.44710.4260.10611.39260.63261.4294218.857687.6193164.4726
313.8285-0.7368-0.45615.3271.04821.0548-0.03330.1676-0.56070.21580.13270.34860.1257-0.0767-0.09940.64960.48690.31061.3660.64671.1627116.17491.867146.8382
322.80071.96746.93022.8864.892819.37120.01830.2509-0.13630.4419-0.09890.36660.72890.33950.08060.8720.48980.26851.13730.36471.1769106.5158124.95257.8991
334.3564-0.37380.63485.5847-0.2933.89990.21910.16570.2339-0.7055-0.2714-0.49490.34430.37840.05230.68090.35550.41591.33940.73091.2746120.9679106.352195.9917
347.54411.2018-0.46423.45520.38927.48850.3958-0.0253-0.6852-0.1847-0.4318-0.53770.05170.24740.0360.65560.32330.1061.05120.48191.4134125.95392.3427118.5295
350.4069-0.8317-0.95968.3157-0.14865.0607-0.1068-0.10070.51160.8080.043-0.6496-0.4550.63370.06380.92070.0183-0.26311.22340.39781.7016130.0996128.8702128.9227
363.6212.06070.2173.52010.28410.5364-0.01910.2616-0.11820.05190.0597-0.0748-0.03780.0508-0.04060.46160.4010.26161.44120.62991.3176142.3327120.23744.9059
370.4951-0.2476-0.48823.1124-0.43336.411-0.01570.08550.7833-0.16550.11780.2925-0.095-0.2932-0.1020.39730.33170.18981.34540.71281.812107.514120.4092103.3754
381.23340.06941.07371.32251.76693.2021-0.1616-0.630.4602-0.0997-0.25250.0572-0.5236-0.81840.41411.30010.320.29661.16760.28831.792109.324134.9447127.0782
390.5354-0.8470.0732.5845-0.06550.92940.0790.3697-0.17240.308-0.1622-0.15720.1642-0.33340.08330.81170.13250.16541.45060.74271.6569112.251936.3792161.1871
4010.2797-5.229111.14464.6876-6.535512.97430.413-0.1534-0.0226-0.17150.0047-0.15650.7342-0.4051-0.41780.88860.3160.34041.42630.29571.056397.684678.7495153.91
417.88651.977-0.67523.30911.49683.16810.43130.3189-0.21350.0939-0.29470.22560.742-0.1643-0.13660.86710.27280.26911.1470.56021.269494.628982.2557114.1552
423.97420.42720.18134.2085-0.37661.6454-0.71220.5184-0.2032-0.77650.04190.4270.6168-0.03120.67031.430.09280.34461.43390.23551.194797.416884.908587.4833
430.26480.08280.49930.04130.17491.0547-0.1234-0.033-0.172-0.0928-0.01630.1105-0.08930.14550.13971.0394-0.070.19741.69860.42562.660461.321493.954293.2094
442.4271-0.2351-1.04053.1924-0.56011.49750.1775-0.0623-0.29840.5674-0.34650.4084-0.09350.06720.1690.64480.19280.32791.42720.60561.424281.296255.157173.2568
450.68830.77051.6967.675-2.77.9726-0.1436-0.16420.2411-0.2571-0.16980.78840.1535-0.56830.31340.42360.22690.3541.38290.40211.610985.9908102.1544116.8156
465.15573.69480.51592.69960.47340.7274-0.81410.30091.2807-0.52320.43131.1285-0.1541-0.09050.38280.69270.4119-0.28181.69560.8832.116964.5472112.461103.3298
472.63792.21271.79975.84811.24693.93050.258-0.1644-0.16930.48950.43570.42310.4642-0.4549-0.69380.4229-0.39450.21841.5320.79761.596253.484292.8763255.3594
488.3202-2.30371.30178.2035-3.17184.19260.4874-0.3124-0.44370.2549-0.11930.4963-0.2767-0.0218-0.36810.3205-0.02080.281.52830.52141.053781.6688112.691245.9236
493.81061.8962.31115.9671-0.11591.828-0.447-0.405-0.00560.13520.35810.0424-0.545-0.33270.08890.56550.18440.28661.71160.51861.130297.6454126.1239236.468
504.4858-4.0018-2.97994.3211.0497.3154-0.213-0.04520.05280.1720.0152-0.6112-0.3613-0.2220.19790.6904-0.08140.31421.58990.3781.0469106.4219123.538222.58
514.92.08093.65556.112-0.68125.69510.1325-0.2280.3013-0.31920.337-0.10350.7526-0.1382-0.46960.84870.02420.35671.55560.38220.9848116.5189123.727204.3951
524.67552.513-0.40197.3324-0.27772.396-0.52510.9928-1.0182-0.72170.5635-0.1285-0.6141.0078-0.03840.6280.10210.41642.03820.31940.895135.7485133.0058199.8654
536.05641.50391.71991.01630.28131.2994-0.56970.02710.4863-0.37670.62270.2104-0.77420.9272-0.0531.4406-0.42980.59572.55140.34970.8031143.7248149.0987207.1512
547.78522.47851.51492.66220.28863.7182-0.3053-0.4828-0.0806-0.00170.1305-0.07270.05220.59330.17490.77420.27130.22331.68670.40580.9064139.1062141.1906228.2574
550.8471.1011-1.26848.24213.23935.52790.2017-0.25660.07260.2139-0.0012-0.0058-0.0710.5074-0.20050.82640.25870.04231.87730.12350.9948129.7536146.6676248.0771
561.6059-0.9088-0.56165.9336-0.56770.49430.3363-0.63140.2014-0.9103-0.4571-0.0847-0.22950.08050.12091.02990.30520.13211.6492-0.18151.2515109.2127154.4897256.5952
571.15471.52711.59842.2732.46452.72550.3069-0.19560.4125-0.019-0.23020.1353-0.2099-0.4364-0.07671.21460.48510.37641.68210.10121.946894.3408159.5423255.195
580.48421.25810.05375.25712.61275.79360.15780.10850.12720.17550.2687-0.96280.1120.5784-0.42650.17390.34250.37981.86680.58321.4298133.4689124.0478172.3342
598.4864-0.1712-1.83390.4379-1.84358.84060.65490.15640.07040.0739-0.07660.1207-0.56150.3122-0.57830.81250.1670.4291.47890.38210.9424109.8146129.4453178.9276
604.88183.9084-1.179410.2211-0.99127.19770.2990.24830.01880.3669-0.04270.0957-0.36770.0696-0.25620.63660.35280.3671.53970.61360.894792.774129.9966190.661
617.0068-0.136-1.67634.523-3.19323.54340.3274-0.01310.0048-0.2053-0.10410.3668-0.1579-0.0094-0.22340.60340.23120.10131.42540.5911.006980.0412122.9563207.0654
627.23213.6592-5.53026.0281-1.66194.6865-0.11330.38870.0304-0.24080.33520.38750.2596-0.3879-0.22190.5326-0.1408-0.19311.56650.83141.189562.1929102.7719228.4306
632.24351.0268-0.41873.66442.66182.8811-0.07880.3702-0.6783-0.5822-0.47340.6858-0.6867-0.63420.55221.00380.1825-0.52231.65410.23652.017528.0159106.1574229.18
640.0062-0.0044-0.01760.08280.06410.09040.0891-0.035-0.0244-0.113-0.27160.3205-0.338-0.19040.18251.71220.43480.10092.73210.2112.267231.7197129.2598209.4945
650.1153-0.0099-0.08790.00130.00390.1020.450.05860.4134-0.0486-0.0245-0.0212-0.27960.1869-0.42552.41830.04320.96622.2079-0.05572.188538.9627154.1439219.9754
660.0176-0.00150.01030.0015-0.00160.00670.06780.0139-0.15150.02970.04220.0390.00020.0018-0.112.5057-0.07850.58922.34220.22411.987758.9166165.4045225.0795
676.6941.6456-0.65110.94280.81542.0632-0.11260.7215-0.5528-0.0749-0.02770.04740.1524-0.44310.14030.60710.31060.38081.99230.58411.432688.6392112.072167.3954
683.98970.69330.42673.7111-1.53471.7193-0.48380.6247-0.21360.320.2891-0.04160.7533-0.55670.19471.251-0.27560.34441.54910.84282.044978.689670.4732235.6391
691.9934-1.3435-0.44341.44570.51030.3371-0.36060.2359-1.18210.23520.2287-0.0085-0.19450.51830.13192.0974-0.0173-0.16891.8065-0.25692.1227102.889157.419230.1061
Refinement TLS group
IDRefine-IDRefine TLS-IDAuth asym-IDAuth seq-ID
1ELECTRON MICROSCOPY1C1 - 247
2ELECTRON MICROSCOPY2C248 - 343
3ELECTRON MICROSCOPY2E151 - 181
4ELECTRON MICROSCOPY3C344 - 558
5ELECTRON MICROSCOPY4E12 - 150
6ELECTRON MICROSCOPY5F1 - 183
7ELECTRON MICROSCOPY6F184 - 355
8ELECTRON MICROSCOPY7L1 - 103
9ELECTRON MICROSCOPY8L104 - 197
10ELECTRON MICROSCOPY9L198 - 296
11ELECTRON MICROSCOPY10L297 - 402
12ELECTRON MICROSCOPY11M1 - 103
13ELECTRON MICROSCOPY12M104 - 197
14ELECTRON MICROSCOPY13M198 - 296
15ELECTRON MICROSCOPY14M297 - 402
16ELECTRON MICROSCOPY15G12 - 342
17ELECTRON MICROSCOPY15S32 - 55
18ELECTRON MICROSCOPY16G343 - 701
19ELECTRON MICROSCOPY16S19 - 31
20ELECTRON MICROSCOPY17S56 - 246
21ELECTRON MICROSCOPY18S265 - 524
22ELECTRON MICROSCOPY19S542 - 627
23ELECTRON MICROSCOPY20S648 - 1033
24ELECTRON MICROSCOPY20S2001 - 2107
25ELECTRON MICROSCOPY21S1043 - 1215
26ELECTRON MICROSCOPY22S1216 - 1443
27ELECTRON MICROSCOPY23H12 - 386
28ELECTRON MICROSCOPY23A29 - 55
29ELECTRON MICROSCOPY24H387 - 610
30ELECTRON MICROSCOPY24A19 - 28
31ELECTRON MICROSCOPY25A56 - 248
32ELECTRON MICROSCOPY26A262 - 401
33ELECTRON MICROSCOPY26B811 - 827
34ELECTRON MICROSCOPY27A402 - 524
35ELECTRON MICROSCOPY28A648 - 1034
36ELECTRON MICROSCOPY29A1043 - 1215
37ELECTRON MICROSCOPY30A1216 - 1443
38ELECTRON MICROSCOPY31B7 - 369
39ELECTRON MICROSCOPY32B385 - 431
40ELECTRON MICROSCOPY32P446 - 500
41ELECTRON MICROSCOPY33B471 - 655
42ELECTRON MICROSCOPY34B656 - 749
43ELECTRON MICROSCOPY34P672 - 685
44ELECTRON MICROSCOPY35B750 - 783
45ELECTRON MICROSCOPY35B828 - 859
46ELECTRON MICROSCOPY35P823 - 881
47ELECTRON MICROSCOPY36P5 - 435
48ELECTRON MICROSCOPY37P501 - 659
49ELECTRON MICROSCOPY38P701 - 822
50ELECTRON MICROSCOPY39O7 - 369
51ELECTRON MICROSCOPY40O385 - 432
52ELECTRON MICROSCOPY40Q449 - 500
53ELECTRON MICROSCOPY41O471 - 655
54ELECTRON MICROSCOPY42O656 - 749
55ELECTRON MICROSCOPY42Q669 - 685
56ELECTRON MICROSCOPY43O750 - 859
57ELECTRON MICROSCOPY43Q823 - 881
58ELECTRON MICROSCOPY44Q5 - 435
59ELECTRON MICROSCOPY45Q501 - 668
60ELECTRON MICROSCOPY46Q701 - 822
61ELECTRON MICROSCOPY47U1 - 169
62ELECTRON MICROSCOPY48U170 - 305
63ELECTRON MICROSCOPY49U306 - 399
64ELECTRON MICROSCOPY50U408 - 476
65ELECTRON MICROSCOPY51U477 - 550
66ELECTRON MICROSCOPY52U575 - 684
67ELECTRON MICROSCOPY53U696 - 800
68ELECTRON MICROSCOPY54U801 - 934
69ELECTRON MICROSCOPY55U949 - 1039
70ELECTRON MICROSCOPY56U1040 - 1154
71ELECTRON MICROSCOPY57U1155 - 1280
72ELECTRON MICROSCOPY58V45 - 187
73ELECTRON MICROSCOPY59V188 - 254
74ELECTRON MICROSCOPY60V255 - 311
75ELECTRON MICROSCOPY61V337 - 465
76ELECTRON MICROSCOPY62V466 - 623
77ELECTRON MICROSCOPY63V624 - 835
78ELECTRON MICROSCOPY64V929 - 1145
79ELECTRON MICROSCOPY65V1150 - 1250
80ELECTRON MICROSCOPY66V1251 - 1384
81ELECTRON MICROSCOPY67E275 - 535
82ELECTRON MICROSCOPY68X1 - 153
83ELECTRON MICROSCOPY69Y1 - 22
84ELECTRON MICROSCOPY69Z37 - 58

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