- EMDB-23090: Structure of the human fanconi anaemia Core-UBE2T-ID-DNA complex ... -
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基本情報
登録情報
データベース: EMDB / ID: EMD-23090
タイトル
Structure of the human fanconi anaemia Core-UBE2T-ID-DNA complex in closed state
マップデータ
composite REFMAC5 map (masked) used in coordinate refinement
試料
複合体: Human Fanconi Anaemia Core-UBE2T-ID-DNA complex
タンパク質・ペプチド: x 12種
DNA: x 2種
リガンド: x 1種
キーワード
Complex / LIGASE / LIGASE-DNA complex
機能・相同性
機能・相同性情報
regulation of germ cell proliferation / regulation of CD40 signaling pathway / Fanconi anaemia nuclear complex / protein K27-linked ubiquitination / regulation of regulatory T cell differentiation / protein K29-linked ubiquitination / male meiotic nuclear division / gamete generation / double-strand break repair involved in meiotic recombination / homologous chromosome pairing at meiosis ...regulation of germ cell proliferation / regulation of CD40 signaling pathway / Fanconi anaemia nuclear complex / protein K27-linked ubiquitination / regulation of regulatory T cell differentiation / protein K29-linked ubiquitination / male meiotic nuclear division / gamete generation / double-strand break repair involved in meiotic recombination / homologous chromosome pairing at meiosis / neuronal stem cell population maintenance / replication-born double-strand break repair via sister chromatid exchange / protein K6-linked ubiquitination / brain morphogenesis / protein K11-linked ubiquitination / mitotic intra-S DNA damage checkpoint signaling / DNA repair complex / myeloid cell homeostasis / E2 ubiquitin-conjugating enzyme / female gonad development / spermatid development / germ cell development / ubiquitin conjugating enzyme activity / homeostasis of number of cells / protein K63-linked ubiquitination / protein monoubiquitination / negative regulation of double-strand break repair via homologous recombination / positive regulation of double-strand break repair via homologous recombination / interstrand cross-link repair / protein K48-linked ubiquitination / protein autoubiquitination / ovarian follicle development / condensed chromosome / DNA polymerase binding / removal of superoxide radicals / Synthesis of active ubiquitin: roles of E1 and E2 enzymes / response to gamma radiation / nucleotide-excision repair / mitochondrion organization / TP53 Regulates Transcription of DNA Repair Genes / Fanconi Anemia Pathway / response to radiation / RING-type E3 ubiquitin transferase / PKR-mediated signaling / male gonad development / protein polyubiquitination / ubiquitin-protein transferase activity / ubiquitin protein ligase activity / nuclear envelope / regulation of cell population proliferation / chromosome / cellular response to oxidative stress / protein-containing complex assembly / regulation of inflammatory response / gene expression / damaged DNA binding / nuclear speck / nuclear body / DNA repair / intracellular membrane-bounded organelle / centrosome / ubiquitin protein ligase binding / DNA damage response / chromatin binding / chromatin / nucleolus / mitochondrion / DNA binding / zinc ion binding / nucleoplasm / ATP binding / nucleus / cytosol / cytoplasm 類似検索 - 分子機能
Fanconi anaemia group C protein / Fanconi anemia-associated protein of 100kDa / Fanconi anemia group B protein / Fanconi anemia group G protein / Fanconi anaemia group C protein / Fanconi anemia-associated / Fanconi Anaemia group E protein, C-terminal / Fanconi anemia group F protein / FANCF, C-terminal domain superfamily / Fanconi anemia group E protein ...Fanconi anaemia group C protein / Fanconi anemia-associated protein of 100kDa / Fanconi anemia group B protein / Fanconi anemia group G protein / Fanconi anaemia group C protein / Fanconi anemia-associated / Fanconi Anaemia group E protein, C-terminal / Fanconi anemia group F protein / FANCF, C-terminal domain superfamily / Fanconi anemia group E protein / Fanconi anemia group F protein (FANCF) / Fanconi Anaemia group E protein FANCE / Fanconi anaemia group A protein / Fanconi anaemia group A protein, N-terminal domain / : / : / : / Fanconi anaemia group A protein C-terminal domain / Fanconi anaemia group A protein N terminus / FANCA helical domain / FANCA arcN subdomain / Fanconi anemia complex, subunit FancL, WD-repeat containing domain / E3 ubiquitin-protein ligase FANCL / FANCL C-terminal domain / FANCL, UBC-like domain 3 superfamily / FANCL, UBC-like domain 2 / FANCL, UBC-like domain 3 / FANCL UBC-like domain 1 / FANCL C-terminal domain / FANCL UBC-like domain 2 / FANCL UBC-like domain 3 / FANCL C-terminal domain / Fanconi anemia group I protein / FANCI solenoid 1 cap / FANCI solenoid 1 domain / FANCI helical domain 1 / FANCI helical domain 2 / FANCI solenoid 3 domain / FANCI solenoid 4 domain / FANCI solenoid 2 domain / FANCI solenoid 1 cap / FANCI solenoid 1 / FANCI solenoid 2 / FANCI solenoid 3 / FANCI solenoid 4 / FANCI helical domain 1 / FANCI helical domain 2 / Fanconi anaemia protein FANCD2 / Fanconi anaemia protein FancD2 nuclease / : / Ubiquitin-conjugating enzyme, active site / Ubiquitin-conjugating (UBC) active site signature. / Ubiquitin-conjugating enzyme E2 / Ubiquitin-conjugating enzyme / Ubiquitin-conjugating (UBC) core domain profile. / Ubiquitin-conjugating enzyme E2, catalytic domain homologues / Ubiquitin-conjugating enzyme/RWD-like / Tetratricopeptide repeats / Tetratricopeptide repeat / Tetratricopeptide-like helical domain superfamily / Zinc finger, RING/FYVE/PHD-type 類似検索 - ドメイン・相同性
Fanconi anemia, complementation group I / Fanconi anemia group G protein / Fanconi anemia group A protein / Fanconi anemia group C protein / Fanconi anemia core complex-associated protein 100 / Fanconi anemia group B protein / Fanconi anemia group D2 protein / Fanconi anemia group E protein / Ubiquitin-conjugating enzyme E2 T / Fanconi anemia group F protein / E3 ubiquitin-protein ligase FANCL 類似検索 - 構成要素
ジャーナル: Nat Struct Mol Biol / 年: 2021 タイトル: Structure of the FA core ubiquitin ligase closing the ID clamp on DNA. 著者: Shengliu Wang / Renjing Wang / Christopher Peralta / Ayat Yaseen / Nikola P Pavletich / 要旨: The Fanconi anemia (FA) pathway is essential for the repair of DNA interstrand crosslinks. Central to the pathway is the FA core complex, a ubiquitin ligase of nine subunits that monoubiquitinates ...The Fanconi anemia (FA) pathway is essential for the repair of DNA interstrand crosslinks. Central to the pathway is the FA core complex, a ubiquitin ligase of nine subunits that monoubiquitinates the FANCI-FANCD2 (ID) DNA clamp. The 3.1 Å structure of the 1.1-MDa human FA core complex, described here, reveals an asymmetric assembly with two copies of all but the FANCC, FANCE and FANCF subunits. The asymmetry is crucial, as it prevents the binding of a second FANCC-FANCE-FANCF subcomplex that inhibits the recruitment of the UBE2T ubiquitin conjugating enzyme, and instead creates an ID binding site. A single active site then ubiquitinates FANCD2 and FANCI sequentially. We also present the 4.2-Å structures of the human core-UBE2T-ID-DNA complex in three conformations captured during monoubiquitination. They reveal the core-UBE2T complex remodeling the ID-DNA complex, closing the clamp on the DNA before ubiquitination. Monoubiquitination then prevents clamp opening after release from the core.
focus2: reconstruction (masked) focused at FANCA' NTD, FANCG'-Ectd-L', FANCB-FAAP100 dimer of heterodimers, the ID-DNA complex and UBE2T postprocessed with relion