CRISPR-associated protein Csy1 / CRISPR-associated protein (Cas_Csy1) / CRISPR-associated protein Csy2 / CRISPR-associated protein (Cas_Csy2) / CRISPR-associated protein Csy3 / CRISPR-associated protein (Cas_Csy3) 類似検索 - ドメイン・相同性
: / DNA / DNA (> 10) / RNA / RNA (> 10) / Tail protein / Uncharacterized protein / CRISPR-associated protein Csy3 / Uncharacterized protein 類似検索 - 構成要素
ジャーナル: Nucleic Acids Res / 年: 2021 タイトル: Insights into the dual functions of AcrIF14 during the inhibition of type I-F CRISPR-Cas surveillance complex. 著者: Xi Liu / Laixing Zhang / Yu Xiu / Teng Gao / Ling Huang / Yongchao Xie / Lingguang Yang / Wenhe Wang / Peiyi Wang / Yi Zhang / Maojun Yang / Yue Feng / 要旨: CRISPR-Cas systems are bacterial adaptive immune systems, and phages counteract these systems using many approaches such as producing anti-CRISPR (Acr) proteins. Here, we report the structures of ...CRISPR-Cas systems are bacterial adaptive immune systems, and phages counteract these systems using many approaches such as producing anti-CRISPR (Acr) proteins. Here, we report the structures of both AcrIF14 and its complex with the crRNA-guided surveillance (Csy) complex. Our study demonstrates that apart from interacting with the Csy complex to block the hybridization of target DNA to the crRNA, AcrIF14 also endows the Csy complex with the ability to interact with non-sequence-specific dsDNA as AcrIF9 does. Further structural studies of the Csy-AcrIF14-dsDNA complex and biochemical studies uncover that the PAM recognition loop of the Cas8f subunit of the Csy complex and electropositive patches within the N-terminal domain of AcrIF14 are essential for the non-sequence-specific dsDNA binding to the Csy-AcrIF14 complex, which is different from the mechanism of AcrIF9. Our findings highlight the prevalence of Acr-induced non-specific DNA binding and shed light on future studies into the mechanisms of such Acr proteins.
履歴
登録
2021年3月13日
登録サイト: PDBJ / 処理サイト: PDBJ
改定 1.0
2021年11月17日
Provider: repository / タイプ: Initial release
改定 1.1
2024年6月5日
Group: Data collection / カテゴリ: chem_comp_atom / chem_comp_bond
#89 - 2007年5月 アコニターゼと鉄調節タンパク質1 (Aconitase and Iron Regulatory Protein 1) 類似性 (1)
#241 - 2020年1月 20年の分子を振り返って (Twenty Years of Molecules) 類似性 (2)
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集合体
登録構造単位
A: Type I-F CRISPR-associated protein Csy1 B: CRISPR type I-F/YPEST-associated protein Csy2 C: CRISPR-associated protein Csy3 D: CRISPR-associated protein Csy3 E: CRISPR-associated protein Csy3 F: CRISPR-associated protein Csy3 G: CRISPR-associated protein Csy3 H: CRISPR-associated protein Csy3 I: AcrIF14 J: AcrIF14 M: RNA (60-MER) N: 54-MER DNA O: 54-MER DNA