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Yorodumi- PDB-7bj2: Salmonella flagellar basal body assembly intermediate - P ring al... -
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-Basic information
Entry | Database: PDB / ID: 7bj2 | |||||||||||||||
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Title | Salmonella flagellar basal body assembly intermediate - P ring alone structure | |||||||||||||||
Components | Flagellar P-ring protein | |||||||||||||||
Keywords | PROTEIN TRANSPORT / bacterial flagellum rod bacterial flagellum export gate basal body | |||||||||||||||
Function / homology | bacterial-type flagellum basal body, distal rod, P ring / Flagellar P-ring protein / Flagellar P-ring protein / bacterial-type flagellum-dependent cell motility / outer membrane-bounded periplasmic space / structural molecule activity / Flagellar P-ring protein Function and homology information | |||||||||||||||
Biological species | Salmonella typhimurium (bacteria) | |||||||||||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3 Å | |||||||||||||||
Authors | Johnson, S. / Furlong, E. / Lea, S.M. | |||||||||||||||
Funding support | United Kingdom, 4items
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Citation | Journal: Nat Microbiol / Year: 2021 Title: Molecular structure of the intact bacterial flagellar basal body. Authors: Steven Johnson / Emily J Furlong / Justin C Deme / Ashley L Nord / Joseph J E Caesar / Fabienne F V Chevance / Richard M Berry / Kelly T Hughes / Susan M Lea / Abstract: The bacterial flagellum is a macromolecular protein complex that enables motility in many species. Bacterial flagella self-assemble a strong, multicomponent drive shaft that couples rotation in the ...The bacterial flagellum is a macromolecular protein complex that enables motility in many species. Bacterial flagella self-assemble a strong, multicomponent drive shaft that couples rotation in the inner membrane to the micrometre-long flagellar filament that powers bacterial swimming in viscous fluids. Here, we present structures of the intact Salmonella flagellar basal body, encompassing the inner membrane rotor, drive shaft and outer-membrane bushing, solved using cryo-electron microscopy to resolutions of 2.2-3.7 Å. The structures reveal molecular details of how 173 protein molecules of 13 different types assemble into a complex spanning two membranes and a cell wall. The helical drive shaft at one end is intricately interwoven with the rotor component with both the export gate complex and the proximal rod forming interactions with the MS-ring. At the other end, the drive shaft distal rod passes through the LP-ring bushing complex, which functions as a molecular bearing anchored in the outer membrane through interactions with the lipopolysaccharide. The in situ structure of a protein complex capping the drive shaft provides molecular insights into the assembly process of this molecular machine. | |||||||||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | Molecule: MolmilJmol/JSmol |
-Downloads & links
-Download
PDBx/mmCIF format | 7bj2.cif.gz | 1.2 MB | Display | PDBx/mmCIF format |
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PDB format | pdb7bj2.ent.gz | 1 MB | Display | PDB format |
PDBx/mmJSON format | 7bj2.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7bj2_validation.pdf.gz | 1.4 MB | Display | wwPDB validaton report |
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Full document | 7bj2_full_validation.pdf.gz | 1.4 MB | Display | |
Data in XML | 7bj2_validation.xml.gz | 182.8 KB | Display | |
Data in CIF | 7bj2_validation.cif.gz | 251.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/bj/7bj2 ftp://data.pdbj.org/pub/pdb/validation_reports/bj/7bj2 | HTTPS FTP |
-Related structure data
Related structure data | 12193MC 7bglC 7bhqC 7binC 7bk0C 7nvgC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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Noncrystallographic symmetry (NCS) | NCS domain:
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