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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 7abg | ||||||
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タイトル | Human pre-Bact-1 spliceosome | ||||||
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![]() | SPLICING / Complex / spliceosome / catalytic activation | ||||||
機能・相同性 | ![]() snRNA export from nucleus / nuclear cap binding complex / DNA topoisomerase binding / mRNA metabolic process / RNA cap binding complex / histone mRNA metabolic process / microfibril / positive regulation of mRNA 3'-end processing / Lsm2-8 complex / U6 snRNA 3'-end binding ...snRNA export from nucleus / nuclear cap binding complex / DNA topoisomerase binding / mRNA metabolic process / RNA cap binding complex / histone mRNA metabolic process / microfibril / positive regulation of mRNA 3'-end processing / Lsm2-8 complex / U6 snRNA 3'-end binding / positive regulation of RNA export from nucleus / cap-dependent translational initiation / protein kinase B binding / mRNA decay by 5' to 3' exoribonuclease / Lsm1-7-Pat1 complex / U11/U12 snRNP / U6 snRNP / Processing of Intronless Pre-mRNAs / regulation of retinoic acid receptor signaling pathway / PH domain binding / snRNA binding / positive regulation of RNA binding / U2 snRNP binding / U7 snRNA binding / histone pre-mRNA DCP binding / RNA cap binding / WW domain binding / U7 snRNP / alternative mRNA splicing, via spliceosome / B-WICH complex / regulation of vitamin D receptor signaling pathway / histone pre-mRNA 3'end processing complex / cis assembly of pre-catalytic spliceosome / miRNA-mediated post-transcriptional gene silencing / SLBP independent Processing of Histone Pre-mRNAs / primary miRNA processing / regulation of mRNA processing / SLBP Dependent Processing of Replication-Dependent Histone Pre-mRNAs / mRNA splice site recognition / regulatory ncRNA-mediated post-transcriptional gene silencing / splicing factor binding / spliceosome conformational change to release U4 (or U4atac) and U1 (or U11) / protein methylation / U12-type spliceosomal complex / mRNA 3'-end processing / miRNA processing / methylosome / Transport of the SLBP independent Mature mRNA / P-body assembly / Transport of the SLBP Dependant Mature mRNA / RNA 7-methylguanosine cap binding / nuclear retinoic acid receptor binding / Prp19 complex / 7-methylguanosine cap hypermethylation / positive regulation of androgen receptor activity / transcription elongation factor activity / U1 snRNP binding / mRNA 3'-end processing / blastocyst formation / pICln-Sm protein complex / Transport of Mature mRNA Derived from an Intronless Transcript / pre-mRNA binding / U2-type catalytic step 1 spliceosome / RNA splicing, via transesterification reactions / small nuclear ribonucleoprotein complex / sno(s)RNA-containing ribonucleoprotein complex / SMN-Sm protein complex / regulation of mRNA splicing, via spliceosome / spliceosomal tri-snRNP complex / P granule / telomerase holoenzyme complex / U2-type spliceosomal complex / mRNA cis splicing, via spliceosome / positive regulation by host of viral transcription / U2-type precatalytic spliceosome / commitment complex / RNA catabolic process / positive regulation of vitamin D receptor signaling pathway / Transport of Mature mRNA derived from an Intron-Containing Transcript / telomerase RNA binding / transcription regulator inhibitor activity / U2-type prespliceosome assembly / Abortive elongation of HIV-1 transcript in the absence of Tat / nuclear vitamin D receptor binding / U2-type catalytic step 2 spliceosome / ubiquitin-like protein conjugating enzyme binding / U4 snRNP / RNA polymerase binding / regulation of translational initiation / Notch binding / Regulation of gene expression in late stage (branching morphogenesis) pancreatic bud precursor cells / RUNX3 regulates NOTCH signaling / U2 snRNP / SAGA complex / positive regulation of mRNA splicing, via spliceosome / RNA Polymerase II Transcription Termination / NOTCH4 Intracellular Domain Regulates Transcription / RHOBTB1 GTPase cycle / positive regulation of transcription by RNA polymerase III / tRNA processing 類似検索 - 分子機能 | ||||||
生物種 | ![]() synthetic construct (人工物) | ||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 7.8 Å | ||||||
![]() | Townsend, C. / Kastner, B. / Leelaram, M.N. / Bertram, K. / Stark, H. / Luehrmann, R. | ||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Mechanism of protein-guided folding of the active site U2/U6 RNA during spliceosome activation. 著者: Cole Townsend / Majety N Leelaram / Dmitry E Agafonov / Olexandr Dybkov / Cindy L Will / Karl Bertram / Henning Urlaub / Berthold Kastner / Holger Stark / Reinhard Lührmann / ![]() 要旨: Spliceosome activation involves extensive protein and RNA rearrangements that lead to formation of a catalytically active U2/U6 RNA structure. At present, little is known about the assembly pathway ...Spliceosome activation involves extensive protein and RNA rearrangements that lead to formation of a catalytically active U2/U6 RNA structure. At present, little is known about the assembly pathway of the latter and the mechanism whereby proteins aid its proper folding. Here, we report the cryo-electron microscopy structures of two human, activated spliceosome precursors (that is, pre-B complexes) at core resolutions of 3.9 and 4.2 angstroms. These structures elucidate the order of the numerous protein exchanges that occur during activation, the mutually exclusive interactions that ensure the correct order of ribonucleoprotein rearrangements needed to form the U2/U6 catalytic RNA, and the stepwise folding pathway of the latter. Structural comparisons with mature B complexes reveal the molecular mechanism whereby a conformational change in the scaffold protein PRP8 facilitates final three-dimensional folding of the U2/U6 catalytic RNA. | ||||||
履歴 |
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構造の表示
ムービー |
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構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 2.3 MB | 表示 | ![]() |
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PDB形式 | ![]() | 表示 | ![]() | |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 1.6 MB | 表示 | ![]() |
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文書・詳細版 | ![]() | 1.6 MB | 表示 | |
XML形式データ | ![]() | 251.1 KB | 表示 | |
CIF形式データ | ![]() | 447.4 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 11695MC ![]() 7aavC ![]() 7abfC ![]() 7abhC ![]() 7abiC M: このデータのモデリングに利用したマップデータ C: 同じ文献を引用 ( |
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類似構造データ | |
電子顕微鏡画像生データ | ![]() Data #1: Motion-corrected micrographs (without dose-weighting) of human pre-Bact spliceosome [micrographs - single frame] Data #2: Motion-corrected micrographs (with dose-weighting) of human pre-Bact spliceosome [micrographs - single frame]) |
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リンク
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集合体
登録構造単位 | ![]()
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要素
+Nuclear cap-binding protein subunit ... , 2種, 2分子 A5A1
+U6 snRNA-associated Sm-like protein ... , 7種, 7分子 A2V9CHJA3
+Splicing factor 3B subunit ... , 6種, 6分子 zuTEwx
+Splicing factor 3A subunit ... , 3種, 3分子 Fp4
+U5 small nuclear ribonucleoprotein ... , 2種, 2分子 Ds
+U2 small nuclear ribonucleoprotein ... , 2種, 2分子 WB
+タンパク質 , 17種, 18分子 QLRKyGAA4v0NrYA6mfqX
+RNA鎖 , 4種, 4分子 52Z6
+Pre-mRNA-splicing factor ... , 2種, 2分子 IP
+Small nuclear ribonucleoprotein ... , 6種, 12分子 haiblekdjcng
+非ポリマー , 4種, 4分子 ![](data/chem/img/IHP.gif)
![](data/chem/img/GTP.gif)
![](data/chem/img/MG.gif)
![](data/chem/img/GTG.gif)
![](data/chem/img/GTP.gif)
![](data/chem/img/MG.gif)
![](data/chem/img/GTG.gif)
+詳細
-実験情報
-実験
実験 | 手法: 電子顕微鏡法 |
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EM実験 | 試料の集合状態: PARTICLE / 3次元再構成法: 単粒子再構成法 |
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試料調製
構成要素 |
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分子量 | 単位: MEGADALTONS / 実験値: NO | ||||||||||||||||||
由来(天然) | 生物種: ![]() | ||||||||||||||||||
由来(組換発現) | 生物種: synthetic construct (人工物) | ||||||||||||||||||
緩衝液 | pH: 7.9 | ||||||||||||||||||
試料 | 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES | ||||||||||||||||||
試料支持 | グリッドの材料: COPPER / グリッドのタイプ: Quantifoil R3.5/1 | ||||||||||||||||||
急速凍結 | 装置: FEI VITROBOT MARK IV / 凍結剤: ETHANE |
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電子顕微鏡撮影
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
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顕微鏡 | モデル: FEI TITAN KRIOS |
電子銃 | 電子線源: ![]() |
電子レンズ | モード: BRIGHT FIELD |
撮影 | 平均露光時間: 1 sec. / 電子線照射量: 2.25 e/Å2 / 検出モード: INTEGRATING フィルム・検出器のモデル: FEI FALCON III (4k x 4k) |
画像スキャン | 横: 4096 / 縦: 4096 |
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解析
EMソフトウェア |
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CTF補正 | タイプ: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||
3次元再構成 | 解像度: 7.8 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 84539 / 対称性のタイプ: POINT | ||||||||||||||||||||
原子モデル構築 | プロトコル: RIGID BODY FIT / 空間: REAL |